Nucleic acid extraction equipment

Abstract

The utility model discloses nucleic acid extraction equipment which comprises a reagent tube carrying frame, an ultrasonic mechanism and a clamping mechanism, and the ultrasonic mechanism is arranged on one transverse side of the reagent tube carrying frame and used for outputting ultrasonic waves to a reagent in a reagent tube; the clamping mechanism comprises a first clamping piece, a second clamping piece and an elastic assembly, the first clamping piece is transversely and movably arranged on the reagent tube carrying frame and located on one side of the containing space, the second clamping piece is transversely and movably arranged on the reagent tube carrying frame and located on the other side of the containing space, and the second clamping piece is connected with the first clamping piece; the elastic assembly is used for adjusting the width between the first clamping piece and the second clamping piece so as to clamp the reagent tube. According to the nucleic acid extraction equipment, the ultrasonic cracking effect and efficiency are ensured, and the use experience feeling of operators is improved.

Description

TECHNICAL FIELD

[0001] The application belongs to the technical field of nucleic acid extraction, and particularly relates to a nucleic acid extraction device. BACKGROUND

[0002] The nucleic acid extraction device (such as a nucleic acid extraction instrument) belongs to the field of molecular detection devices and is widely applied in various fields such as disease control centers, clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial detection, food safety detection, animal husbandry and molecular biology research. Before the nucleic acid extraction device performs nucleic acid extraction, a lysis device is used to perform lysis operation on the reagent in the reagent tube. In order to reduce the adverse effects on the internal substances of the cells in the reagent, an ultrasonic mechanism is usually used to perform ultrasonic lysis operation on the reagent in the reagent tube. In the process of ultrasonic lysis, in order to ensure the effect of ultrasonic lysis, the reagent tube needs to be fixed, otherwise the reagent tube in the shaking state will reduce the efficiency of ultrasonic lysis or affect the effect of ultrasonic lysis, but if the reagent tube is fixed too tightly, the reagent tube will be difficult to take out from the reagent tube carrier when needed, which will cause inconvenience and greatly reduce the use experience of the nucleic acid extraction device. CONTENT OF THE UTILITY MODEL

[0003] The application aims to provide a nucleic acid extraction device which ensures the effect and efficiency of ultrasonic lysis and improves the use experience of the operator.

[0004] In order to achieve the above-mentioned purpose, the application provides a nucleic acid extraction device, which comprises:

[0005] A reagent tube carrier, wherein the reagent tube carrier has a containing space which is recessed from the top and used for placing a reagent tube;

[0006] An ultrasonic mechanism, which is arranged on one lateral side of the reagent tube carrier and used for outputting ultrasonic waves to the reagent in the reagent tube;

[0007] A clamping mechanism, which comprises a first clamping piece, a second clamping piece and an elastic assembly, the first clamping piece is arranged on the reagent tube carrier in a transversely movable manner and located on one side of the containing space, the second clamping piece is arranged on the reagent tube carrier in a transversely movable manner and located on the other side of the containing space, the second clamping piece is connected with the first clamping piece, and the elastic assembly is used for adjusting the width between the first clamping piece and the second clamping piece to clamp the reagent tube.

[0008] In the embodiment of the application, the clamping mechanism further comprises a connecting assembly, the connecting assembly is arranged on the reagent tube carrier in a transversely movable manner and connected with the first clamping piece and the second clamping piece at the two lateral ends respectively, one end of the elastic assembly abuts against the first clamping piece, and the other end of the elastic assembly abuts against the reagent tube carrier.

[0009] In the embodiment of the present application, the reagent tube carrier is formed with a first partition and a second partition respectively located at the longitudinal two ends of the reagent tube carrier and between the first clamping member and the second clamping member, the connecting assembly comprises a first connecting member and a second connecting member, the first connecting member is movably arranged on the first partition and has two ends respectively connected to the first clamping member and the second clamping member, the second connecting member is movably arranged on the second partition and has two ends respectively connected to the first clamping member and the second clamping member, and the elastic assembly comprises a first elastic member and a second elastic member, the first elastic member is sleeved on the outer side of the first connecting member and has two ends respectively abutting against the first clamping member and the first partition, and the second elastic member is sleeved on the outer side of the second connecting member and has two ends respectively abutting against the first clamping member and the second partition.

[0010] In the embodiment of the present application, the reagent tube comprises a cylindrical portion and an arc portion located at the bottom end of the cylindrical portion, and the nucleic acid extraction device further comprises a limiting member arranged at the bottom of the accommodation space, and the limiting member is formed with an accommodation groove recessed from top to bottom and used for accommodating part of the arc portion.

[0011] In the embodiment of the present application, the limiting member comprises a base portion and a stop portion, the accommodation groove is formed on the base portion, and the stop portion is arranged at the top of the base portion and located on the side of the base portion away from the ultrasonic mechanism.

[0012] In the embodiment of the present application, the side of the stop portion facing the reagent tube is formed with a first arc-shaped wall capable of abutting against the outer peripheral wall of the reagent tube.

[0013] In the embodiment of the present application, the limiting member is made of a heat-conducting material, and the nucleic acid extraction device further comprises a heating member capable of emitting heat and in contact with the limiting member.

[0014] In the embodiment of the present application, the nucleic acid extraction device further comprises a heat insulation member embedded on the reagent tube carrier and located below the limiting member.

[0015] In the embodiment of the present application, the ultrasonic mechanism comprises a probe mounting seat and an ultrasonic probe, the probe mounting seat is arranged at the transverse side of the reagent tube carrier, the ultrasonic probe is transversely and telescopically arranged on the probe mounting seat, the nucleic acid extraction device further comprises a moving guide rail extending in the longitudinal direction, the reagent tube carrier is movably arranged on the moving guide rail, and the number of reagent tubes is multiple, and the multiple reagent tubes are arranged on the reagent tube carrier and distributed along the extension direction of the moving guide rail.

[0016] In the embodiment of the present application, the nucleic acid extraction device further comprises an extraction device for extracting and transferring nucleic acid in a reagent, and the extraction device comprises:

[0017] a bottom plate, the bottom plate is formed with a mounting area and a sample area distributed side by side in the width direction;

[0018] The first driving member is arranged in the mounting area and is distributed along the length direction of the bottom plate.

[0019] The magnetic rod sleeve assembly is arranged above the sample area and is vertically movable, and the magnetic rod sleeve assembly is drivingly connected with the first driving member.

[0020] The second driving member is arranged above the first driving member, and the arrangement direction of the second driving member is consistent with the length direction of the bottom plate, and in the width direction of the bottom plate, the second driving member overlaps with the first driving member.

[0021] The magnetic rod is arranged above the sample area and is vertically movable, and the magnetic rod is drivingly connected with the second driving member and can be partially inserted into the inside of the magnetic rod sleeve assembly or separated from the magnetic rod sleeve assembly under the driving action of the second driving member.

[0022] According to the above technical scheme, the nucleic acid extraction device comprises a reagent tube carrier, an ultrasonic mechanism and a clamping mechanism. The ultrasonic mechanism is arranged on the lateral side of the reagent tube carrier and is used for outputting ultrasonic waves to the reagent in the reagent tube. The clamping mechanism comprises a first clamping member, a second clamping member and an elastic assembly. The first clamping member is arranged on the reagent tube carrier and located on one side of the accommodation space and can move laterally. The second clamping member is arranged on the reagent tube carrier and located on the other side of the accommodation space and can move laterally. The second clamping member is connected with the first clamping member. The elastic assembly is used for adjusting the width between the first clamping member and the second clamping member to clamp the reagent tube. The nucleic acid extraction device has a simple structure. The arrangement of the above clamping mechanism can not only ensure that the reagent tube is in a stable state during ultrasonic lysis, improve the effect and efficiency of ultrasonic lysis, but also enable the reagent tube to be taken out simply and quickly, improve the reliability and use convenience of the nucleic acid extraction device, and improve the use experience of the operator.

[0023] Other features and advantages of the embodiments of the present application will be described in detail in the following detailed description. BRIEF DESCRIPTION OF DRAWINGS

[0024] The accompanying drawings are included to provide a further understanding of the embodiments of the present application, and constitute a part of the specification, and are used together with the following detailed description to explain the embodiments of the present application, but do not constitute a limitation on the embodiments of the present application. For those skilled in the art, other drawings can be obtained from the structures shown in the drawings without creative labor. In the drawings:

[0025] Figure 1 It is a first perspective partial structure schematic view of the nucleic acid extraction device in the embodiments of the present application;

[0026] Figure 2 It is a partial structure schematic view of the ultrasonic mechanism in the embodiments of the present application;

[0027] Figure 3 FIG. 6 is a second perspective view of the nucleic acid extraction device according to an embodiment of the present application;

[0028] Figure 4 FIG. 7 is a structural view of the probe mounting seat according to an embodiment of the present application;

[0029] Figure 5 FIG. 8 is a first perspective view of the reagent tube carrier and the limiting member according to an embodiment of the present application;

[0030] Figure 6 FIG. 9 is a second perspective view of the reagent tube carrier and the limiting member according to an embodiment of the present application;

[0031] Figure 7 FIG. 10 is a structural view of the nucleic acid extraction device according to an embodiment of the present application;

[0032] Figure 8 FIG. 11 is a first perspective view of the extraction device according to an embodiment of the present application;

[0033] Figure 9 FIG. 12 is a second perspective view of the extraction device according to an embodiment of the present application.

[0034] BRIEF DESCRIPTION OF THE DRAWINGS

[0035] 1 - reagent tube carrier; 101 - accommodation space; 102 - first partition; 103 - second partition; 104 - first avoiding notch; 105 - second avoiding notch; 106 - guide wall; 107 - probe entrance and exit; 2 - ultrasonic mechanism; 201 - probe mounting seat; 202 - ultrasonic probe; 203 - second arc-shaped wall; 204 - driving connecting rod; 205 - third driving member; 206 - connecting part; 207 - mounting part; 208 - mounting space; 209 - mounting groove; 3 - clamping mechanism; 301 - first clamping member; 302 - second clamping member; 303 - connecting assembly; 3031 - first connecting member; 3032 - second connecting member; 4 - reagent tube; 401 - cylindrical part; 402 - arc surface part; 5 - limiting member; 501 - accommodation groove; 502 - base part; 503 - stop part; 504 - first arc-shaped wall; 6 - heat insulation member; 7 - moving guide rail; 8 - elastic buffer assembly; 801 - first connecting rod; 802 - connecting plate; 803 - third elastic member; 9 - driving mechanism; 10 - bottom plate; 1001 - mounting area; 1002 - sample area; 11 - first driving member; 12 - magnet bar sleeve assembly; 13 - second driving member; 14 - magnet bar. DETAILED DESCRIPTION

[0036] The specific embodiments of the present application will be described in detail hereinafter with reference to the drawings. It should be understood that the specific embodiments described herein are merely illustrative and are not intended to limit the present application.

[0037] Embodiments of the present application provide a nucleic acid extraction device, as shown in Figure 1 , Figure 3 and Figure 6 , which comprises:

[0038] a reagent tube carrier 1, the reagent tube carrier 1 having a receiving space 101 recessed from the top and used for placing a reagent tube 4;

[0039] an ultrasonic mechanism 2 arranged at a lateral side of the reagent tube carrier 1 and used for outputting ultrasonic waves to the reagent in the reagent tube 4;

[0040] a clamping mechanism 3 comprising a first clamping piece 301, a second clamping piece 302 and an elastic assembly, the first clamping piece 301 being arranged on the reagent tube carrier 1 in a transversely movable manner and located at one side of the receiving space 101, the second clamping piece 302 being arranged on the reagent tube carrier 1 in a transversely movable manner and located at the other side of the receiving space 101, the second clamping piece 302 being connected with the first clamping piece 301, and the elastic assembly being used for adjusting the width between the first clamping piece 301 and the second clamping piece 302 to clamp the reagent tube 4.

[0041] Specifically, the nucleic acid extraction device in the embodiments can be a nucleic acid extraction instrument, and the reagent tube 4 is placed in the receiving space 101. Before the ultrasonic lysis operation is performed by the ultrasonic mechanism 2, the ultrasonic probe 202 is aligned with the reagent tube 4 containing the reagent (such as a mixture of lysis solution and sample, and the sample contains cells) to be lysed, and then the ultrasonic mechanism 2 is turned on to output ultrasonic waves to the reagent tube 4. The reagent tube 4 transmits the energy of the ultrasonic waves to the reagent in the reagent tube 4. The high-frequency acoustic energy of the ultrasonic waves can break the cells to make the substances (such as nucleic acid) in the cells flow out, thereby laying a foundation for subsequent nucleic acid extraction.

[0042] Further, when the operator installs the reagent tube 4, the operator first places the bottom of the reagent tube 4 into the receiving space 101, and then continues to apply a downward force to the reagent tube 4. Under the action of the force, the reagent tube 4 continues to move downward. During the downward movement of the reagent tube 4, the reagent tube 4 applies a pushing force to the second clamping piece 302 in a direction away from the first clamping piece 301. The first clamping piece 301 and the second clamping piece 302 as a whole move in the direction of the second clamping piece 302, and the elastic assembly is compressed. After the reagent tube 4 is moved into position, the second clamping piece 302 continuously applies a pushing force to the reagent tube 4 under the action of the elastic force of the elastic assembly. The second clamping piece 302 and the vertical side wall of the receiving space 101 away from the second clamping piece 302 jointly clamp the reagent tube 4 to ensure the stability of the reagent tube 4 after installation, so that the reagent tube 4 is in a stable state during the ultrasonic lysis process, and the ultrasonic lysis operation is smoothly performed, which is conducive to improving the effect and efficiency of the ultrasonic lysis.

[0043] When the reagent tube 4 needs to be taken out, the operator applies a pushing force to the first clamping member 301 towards the second clamping member 302, further compresses the elastic assembly to move the first clamping member 301 and the second clamping member 302 as a whole towards the direction where the second clamping member 302 is located, to release the clamping effect of the second clamping member 302 and the vertical side wall of the containing space 101 away from the side of the second clamping member 302 on the reagent tube 4, and then an upward force is applied to the reagent tube 4 to take out the reagent tube 4, so that the reagent tube 4 can be conveniently taken out, and the use convenience and reliability of the nucleic acid extraction equipment are improved.

[0044] The nucleic acid extraction equipment in the embodiment has a simple structure, and the clamping mechanism 3 can ensure that the reagent tube 4 is in a stable state during ultrasonic lysis, improve the effect and efficiency of ultrasonic lysis, and also enable the reagent tube 4 to be conveniently and quickly taken out, thereby improving the reliability, use convenience and use experience of the nucleic acid extraction equipment.

[0045] In an embodiment of the present application, the clamping mechanism 3 further comprises a connecting assembly 303, which is transversely movably arranged on the reagent tube carrier 1 and transversely connected to the first clamping member 301 and the second clamping member 302 at two ends, respectively, one end of the elastic assembly abuts against the first clamping member 301, and the other end of the elastic assembly abuts against the reagent tube carrier 1.

[0046] Specifically, in the process of downward movement of the reagent tube 4, the reagent tube 4 will apply a pushing force to the second clamping member 302 away from the first clamping member 301, the first clamping member 301, the second clamping member 302 and the connecting assembly 303 move as a whole towards the direction where the second clamping member 302 is located, and the elastic assembly is compressed; when the reagent tube 4 needs to be taken out, the operator applies a pushing force to the first clamping member 301 towards the second clamping member 302, further compresses the elastic assembly to move the first clamping member 301, the second clamping member 302 and the connecting assembly 303 as a whole towards the direction where the second clamping member 302 is located, to release the clamping effect of the second clamping member 302 and the vertical side wall of the containing space 101 away from the side of the second clamping member 302 on the reagent tube 4, and then an upward force is applied to the reagent tube 4 to take out the reagent tube 4. The above structure is stable and reliable, which not only ensures the stability of clamping but also facilitates the taking out of the reagent tube 4.

[0047] In an embodiment of the present application, as Figures 5-6As shown, the reagent tube carrier 1 is formed with a first partition 102 and a second partition 103 respectively located at the longitudinal two ends of the reagent tube carrier 1 and between the first clamping member 301 and the second clamping member 302, the connecting assembly 303 includes a first connecting member 3031 and a second connecting member 3032, the first connecting member 3031 is movably threaded on the first partition 102 and has two ends respectively connected to the first clamping member 301 and the second clamping member 302, the second connecting member 3032 is movably threaded on the second partition 103 and has two ends respectively connected to the first clamping member 301 and the second clamping member 302, the elastic assembly includes a first elastic member and a second elastic member, the first elastic member is sleeved on the outside of the first connecting member 3031 and has two ends respectively abutting against the first clamping member 301 and the first partition 102, the second elastic member is sleeved on the outside of the second connecting member 3032 and has two ends respectively abutting against the first clamping member 301 and the second partition 103.

[0048] Specifically, the first reagent tube carrier 1 is formed with a first avoiding notch 104 at one lateral side, the first reagent tube carrier 1 is formed with a second avoiding notch 105 opposite to the first avoiding notch 104 at the other lateral side, the first avoiding notch 104 and the second avoiding notch 105 are both through along the length direction (i.e. the longitudinal direction) of the first reagent tube carrier 1 and are both communicated with the accommodating space 101, the first reagent tube carrier 1 has the first partition 102 between the first longitudinal end of the first avoiding notch 104 and the first longitudinal end of the second avoiding notch 105, and has the second partition 103 between the second longitudinal end of the first avoiding notch 104 and the second longitudinal end of the second avoiding notch 105.

[0049] The first clamping member 301 is arranged at the position of the first avoiding notch 104, the second clamping member 302 is arranged at the position of the second avoiding notch 105, one end of the first connecting member 3031 is connected to the first clamping member 301, the other end of the first connecting member 3031 (such as a bolt) is threaded through the first partition 102 and connected to the second clamping member 302, one end of the second connecting member 3032 (such as a bolt) is connected to the first clamping member 301, the other end of the second connecting member 3032 is threaded through the second partition 103 and connected to the second clamping member 302, the accommodating space 101 is between the first clamping member 301, the second clamping member 302, the first partition 102 and the second partition 103; the first elastic member and the second elastic member can both be selected as springs, when the first elastic member and the second elastic member are compressed or restored to the original state, the first clamping member 301, the second clamping member 302, the first connecting member 3031 and the second connecting member 3032 as a whole can move laterally relative to the reagent tube carrier 1.

[0050] When the reagent tube 4 is installed, the reagent tube 4 continuously moves downward and exerts a pushing force on the second clamping member 302 away from the first clamping member 301, and the first clamping member 301, the second clamping member 302, the first connecting member 3031 and the second connecting member 3032 move as a whole towards the direction of the second clamping member 302, and the first elastic member and the second elastic member are compressed; after the reagent tube 4 is moved downward to the position, the second clamping member 302 continuously exerts a pushing force on the reagent tube 4 under the elastic force of the first elastic member and the second elastic member, and the second clamping member 302 and the vertical side wall of the containing space 101 away from the second clamping member 302 jointly clamp the reagent tube 4. When the reagent tube 4 needs to be taken out, the operator exerts a pushing force on the first clamping member 301 towards the second clamping member 302, further compresses the first elastic member and the second elastic member to make the first clamping member 301, the second clamping member 302, the first connecting member 3031 and the second connecting member 3032 move as a whole towards the direction of the second clamping member 302, so as to release the clamping action of the second clamping member 302 and the vertical side wall of the containing space 101 away from the second clamping member 302 on the reagent tube 4, and then an upward force is exerted on the reagent tube 4 to take out the reagent tube 4.

[0051] Further, a guide wall 106 for guiding the reagent tube 4 is formed on the side wall of the containing space 101 close to the second clamping member 302, and under the guiding action of the guide wall 106, the reagent tube 4 can more easily enter the containing space 101 when the operator installs the reagent tube 4.

[0052] In an embodiment of the present application, the reagent tube 4 comprises a cylindrical portion 401 and an arc surface portion 402 at the bottom end of the cylindrical portion 401, and the nucleic acid extraction device further comprises a limiting member 5 arranged at the bottom of the containing space 101, and the limiting member 5 is formed with a containing groove 501 which is recessed from top to bottom and used for containing part of the arc surface portion 402.

[0053] Specifically, in the present embodiment, the first clamping member 301 and the second clamping member 302 clamp the radial two sides of the cylindrical portion 401, and after the reagent tube 4 is placed, the arc surface portion 402 falls into the containing groove 501, and the arrangement of the containing groove 501 limits the movement of the bottom of the reagent tube 4 to the four directions, and the containing groove 501 and the clamping mechanism 3 jointly act to further reduce the shaking of the reagent tube 4 in the containing space 101, which is conducive to further improving the effect of ultrasonic lysis and subsequent nucleic acid extraction.

[0054] In an embodiment of the present application, the limiting member 5 comprises a base portion 502 and a stop portion 503, the containing groove 501 is formed on the base portion 502, and the stop portion 503 is arranged at the top of the base portion 502 and located on the side of the base portion 502 away from the ultrasonic mechanism 2.

[0055] Specifically, the stop portion 503 in the embodiment can apply a lateral stop force to the reagent tube 4, the ultrasonic mechanism 2 is in contact with the reagent tube 4 from the side of the reagent tube 4 away from the stop portion 503 and outputs ultrasonic waves, when the ultrasonic mechanism 2 outputs the ultrasonic waves, the reagent tube 4 has a tendency to move in a direction away from the ultrasonic mechanism 2 under the action of the ultrasonic waves, in this case, the stop portion 503 plays a stop role on the reagent tube 4, that is, the ultrasonic wave action force and the lateral stop force applied by the ultrasonic mechanism 2 are respectively located on the opposite radial sides of the reagent tube 4, so that the setting of the stop portion 503 can further enhance the stability of the reagent tube 4 when it is in contact with the ultrasonic mechanism 2, which is beneficial to further improve the effect of ultrasonic lysis.

[0056] In an embodiment of the present application, the side of the stop portion 503 facing the reagent tube 4 is formed with a first arc-shaped wall 504 capable of abutting the outer peripheral wall of the reagent tube 4. The setting of the first arc-shaped wall 504 can make the force on the reagent tube 4 more uniform when it is in contact with the stop portion 503, and can avoid damage to the reagent tube 4 due to concentrated force.

[0057] In an embodiment of the present application, the limiting member 5 is made of a heat-conducting material, and the nucleic acid extraction device further comprises a heating member capable of emitting heat and in contact with the limiting member 5.

[0058] Specifically, the limiting member 5 in the embodiment is made of an aluminum alloy material, which has the advantages of low cost and fast heat conduction, and can quickly transfer heat. In this case, the limiting member 5 is a heat-conducting member, and the heat-conducting member in contact with the heating member can quickly transfer the heat emitted by the heating member to the reagent tube 4 to heat the reagent in the reagent tube 4, which can further improve the efficiency of ultrasonic lysis and individually heat and lyse the reagent according to actual needs. In addition, the above setting also realizes the integrated design of the reagent tube carrier 1 and the heating function assembly (the heating function assembly in the embodiment is composed of the heating member and the heat-conducting member), improves the space utilization of the containing space 101, reduces the overall space occupied by the reagent tube carrier 1 and the heating function assembly, and is beneficial to reducing the volume of the nucleic acid extraction device and realizing the miniaturization design of the nucleic acid extraction device, so that the nucleic acid extraction device can be directly placed in a biological safety cabinet.

[0059] In an embodiment of the present application, the nucleic acid extraction device further comprises a heat insulation member 6 embedded on the reagent tube carrier 1 and located below the limiting member 5.

[0060] Specifically, the reagent tube carrier 1 in the embodiment can be made of aluminum alloy material. The reagent tube carrier 1 made of the aluminum alloy material is light in weight and low in cost. The heat insulation member 6 can insulate the heat transfer from the heat generating member and the heat conducting member, so as to avoid the heat transfer from the heat generating member and the heat conducting member to the reagent tube carrier 1, thereby preventing the damage of other components connected to the reagent tube carrier 1 and prolonging the service life of the nucleic acid extraction device. Further, the height of the top surface of the heat insulation member 6 is greater than or equal to the height of the bottom wall of the accommodating space 101. In the case that the height of the top surface of the heat insulation member 6 is greater than the height of the bottom wall of the accommodating space 101, the heat insulation member 6 will not interfere with the reagent tube 4 located on one side of the limiting member 5 (e.g., the length direction side of the limiting member 5).

[0061] In an embodiment of the present application, the ultrasonic mechanism 2 includes a probe mounting seat 201 and an ultrasonic probe 202. The probe mounting seat 201 is arranged on one lateral side of the reagent tube carrier 1, and the ultrasonic probe 202 is arranged on the probe mounting seat 201 in a transverse telescopic manner. The nucleic acid extraction device further includes a moving guide rail 7 extending in a longitudinal direction, and the reagent tube carrier 1 is movably arranged on the moving guide rail 7. The plurality of reagent tubes 4 are arranged on the reagent tube carrier 1 and distributed along the extension direction of the moving guide rail 7.

[0062] Specifically, a probe access opening 107 is formed on the side wall of the accommodating space 101 close to the ultrasonic probe 202. The ultrasonic probe 202 is used to output ultrasonic waves. When ultrasonic lysis is needed, the ultrasonic probe 202 is extended from the probe mounting seat 201 and moves towards the direction of the reagent tubes 4 until the ultrasonic probe 202 contacts the reagent tube 4 corresponding to the reagent that needs to be ultrasonic lysed and outputs ultrasonic waves. The nucleic acid extraction device further includes a bin body (not shown in the figure), and the moving guide rail 7 is arranged inside the bin body. The nucleic acid extraction device further includes a driving mechanism 9 drivingly connected with the reagent tube carrier 1. Under the driving action of the driving mechanism 9, the reagent tube carrier 1 can move along the extension direction of the moving guide rail 7. If ultrasonic lysis is needed for the reagents in the plurality of reagent tubes 4, the reagent tube carrier 1 is driven to move after the reagent in one reagent tube 4 is ultrasonic lysed, so that the next reagent tube 4 containing the reagent to be ultrasonic lysed is aligned with the ultrasonic probe 202. Without arranging a plurality of ultrasonic probes 202 to ultrasonic lyse the reagents in the plurality of reagent tubes 4 one by one, the structure of the ultrasonic mechanism 2 is simplified, the volume of the ultrasonic mechanism 2 is reduced, and the production cost of the nucleic acid extraction device is also reduced. Further, the plurality of reagent tubes 4 in the embodiment are integrally formed, i.e., the plurality of reagent tubes 4 are integrated into a reagent strip.

[0063] In an embodiment of the present application, the ultrasonic mechanism 2 further comprises a driving connecting rod 204 and a third driving member 205, the driving connecting rod 204 is arranged on the probe mounting seat 201 and is threadedly connected with the probe mounting seat 201, and the third driving member 205 is drivingly connected with the driving connecting rod 204.

[0064] Specifically, as shown in Figure 2 and Figure 4 , the probe mounting seat 201 comprises a connecting portion 206 and a mounting portion 207 arranged at the bottom end of the connecting portion 206, the ultrasonic probe 202 is mounted on the mounting portion 207, the third driving member 205 can be an electric motor, the driving connecting rod 204 is connected with the driving end of the third driving member 205, the arrangement direction of the driving connecting rod 204 is consistent with the moving direction of the ultrasonic probe 202, and the connecting portion 206 is sleeved on the driving connecting rod 204 and is threadedly connected with the driving connecting rod 204. When the electric motor rotates, the driving connecting rod 204 is driven to rotate, the connecting portion 206 moves along the axial direction of the driving connecting rod 204, and when the connecting portion 206 moves, the mounting portion 207 and the ultrasonic probe 202 connected with the mounting portion 207 are driven to move, so that the ultrasonic probe 202 approaches or moves away from the reagent tube 4, so as to perform ultrasonic lysis on the reagent in the reagent tube 4.

[0065] In an embodiment of the present application, the side of the ultrasonic probe 202 facing the reagent tube 4 is formed with a second arc-shaped wall 203 for abutting against the outer peripheral wall of the reagent tube 4.

[0066] Specifically, since the ultrasonic probe 202 abuts against the reagent tube 4 through the second arc-shaped wall 203, the contact area between the ultrasonic probe 202 and the reagent tube 4 is large (compared with the contact form of point contact between the ultrasonic probe 202 and the reagent tube 4), so that the ultrasonic probe 202 can directly transmit ultrasonic energy to the reagent tube 4 through the second arc-shaped wall 203, the reagent tube 4 directly and concentrates receives the above-mentioned ultrasonic energy at the position in contact with the second arc-shaped wall 203, that is, the above-mentioned ultrasonic energy is focused at the position corresponding to the second arc-shaped wall 203 in the reagent tube 4 and is transmitted to the reagent with higher efficiency, which improves the intensity of the ultrasonic energy received by the reagent, and further improves the efficiency of ultrasonic lysis of cells in the reagent.

[0067] In an embodiment of the present application, the ultrasonic mechanism 2 further comprises an elastic buffering assembly 8 arranged on the probe mounting seat 201 and used for buffering the ultrasonic probe 202, the arrangement of the elastic buffering assembly 8 can buffer the impact between the ultrasonic probe 202 and the reagent tube 4 when the third driving member 205 fails to drive or the ultrasonic probe 202 has a movement error, avoid rigid impact between the ultrasonic probe 202 and the reagent tube 4, reduce the vibration of the ultrasonic probe 202 and / or the reagent tube 4, and protect the ultrasonic mechanism 2, which is conducive to prolonging the service life of the ultrasonic mechanism 2 and the lysis device.

[0068] In an embodiment of the present application, as shown in Figure 4 The probe mounting seat 201 is formed with a mounting space 208, the mounting space 208 is formed with a mounting groove 209 on the side wall facing the reagent tube 4, and the elastic buffering assembly 8 comprises:

[0069] A first connecting rod 801 is inserted into the probe mounting seat 201 and passes through the mounting space 208;

[0070] A second connecting rod is horizontally spaced apart from the first connecting rod 801, the second connecting rod is inserted into the probe mounting seat 201 and passes through the mounting space 208;

[0071] A connecting plate 802 is movably arranged on the first connecting rod 801 and the second connecting rod and located in the mounting space 208, one end of the ultrasonic probe 202 away from the reagent tube 4 passes through the mounting groove 209 and the connecting plate 802 in sequence and extends away from the reagent tube 4;

[0072] A third elastic member 803 is sleeved on the outside of the first connecting rod 801, one end of the third elastic member 803 abuts against the connecting plate 802, and the other end of the third elastic member 803 abuts against the side wall of the mounting space 208 away from the reagent tube 4;

[0073] A fourth elastic member is sleeved on the outside of the second connecting rod, one end of the fourth elastic member abuts against the connecting plate 802, and the other end of the fourth elastic member abuts against the side wall of the mounting space 208 away from the reagent tube 4.

[0074] Specifically, the mounting space 208 is formed on the mounting portion 207, the first connecting rod 801 and the second connecting rod are respectively located on two sides of the ultrasonic probe 202, the first end of the first connecting rod 801 is clamped on the side wall of the mounting space 208 close to the reagent tube 4, the second end of the first connecting rod 801 sequentially passes through the side wall of the mounting space 208 close to the reagent tube 4, the connecting plate 802 and the side wall of the mounting space 208 away from the reagent tube 4; the first end of the second connecting rod is clamped on the side wall of the mounting space 208 close to the reagent tube 4, and the second end of the second connecting rod sequentially passes through the side wall of the mounting space 208 close to the reagent tube 4, the connecting plate 802 and the side wall of the mounting space 208 away from the reagent tube 4; the connecting plate 802 is sleeved outside the ultrasonic probe 202 and is tightly connected with the ultrasonic probe 202, the connecting plate 802 and the ultrasonic probe 202 are two integrals and can move along the axial direction of the first connecting rod 801 and the second connecting rod, and the third elastic member 803 and the fourth elastic member can be selected as springs. If the ultrasonic probe 202 moves by mistake and collides with the reagent tube 4, the ultrasonic probe 202 can move together with the connecting plate 802 away from the reagent tube 4, at this time, the third elastic member 803 and the fourth elastic member are compressed and absorb the impact energy between the ultrasonic probe 202 and the reagent tube 4, so that the impact energy is not continuously transmitted to cause damage to other parts of the ultrasonic mechanism 2.

[0075] In one embodiment of the present application, as shown in Figures 7-9 the nucleic acid extraction device further comprises an extraction device for extracting and transferring nucleic acid in a reagent, the extraction device comprising:

[0076] a bottom plate 10, the bottom plate 10 being provided with mounting areas 1001 and sample areas 1002 distributed side by side along the width direction;

[0077] a first driving member 11 arranged in the mounting areas 1001 and distributed along the length direction of the bottom plate 10;

[0078] a magnetic rod sleeve assembly 12 arranged above the sample areas 1002 and vertically movable, the magnetic rod sleeve assembly 12 being drivingly connected with the first driving member 11;

[0079] a second driving member 13 arranged above the first driving member 11, the second driving member 13 being arranged in the same direction as the length direction of the bottom plate 10, and in the width direction of the bottom plate 10, the second driving member 13 overlaps with the first driving member 11;

[0080] a magnetic rod 14 arranged above the sample areas 1002 and vertically movable, the magnetic rod 14 being drivingly connected with the second driving member 13 and being capable of partially extending into the interior of the magnetic rod sleeve assembly 12 or being separated from the magnetic rod sleeve assembly 12 under the driving action of the second driving member 13.

[0081] Specifically, the mounting area 1001 and the sample area 1002 are distributed in parallel along the width direction of the bottom plate 10 on the bottom plate 10, and both the mounting area 1001 and the sample area 1002 extend along the length direction of the bottom plate 10, that is, the longitudinal direction in the figure, and the width direction of the bottom plate 10 is the transverse direction in the figure. Figure 7 Figure 7 The moving guide rail 7 is arranged in the sample area 1002, and the reagent tube carrier 1 is movably arranged on the moving guide rail 7. The number of the reagent tubes 4 is multiple, and the multiple reagent tubes 4 are detachably arranged on the reagent tube carrier 1. Different types of solutions, such as lysis solution and washing solution, are stored in the multiple reagent tubes 4, and magnetic beads capable of adsorbing nucleic acid are also pre-placed in the reagent tubes 4.

[0082] The first driving member 11 and the second driving member 13 in the embodiment can be selected as a stepper motor. Under the driving action of the first driving member 11, the magnetic rod sleeve assembly 12 moves up and down above the sample area 1002. Under the driving action of the second driving member 13, the magnetic rod 14 moves up and down above the sample area 1002. By changing the movement speed of the first driving member 11 and the second driving member 13, the magnetic rod sleeve assembly 12 and the magnetic rod 14 can move alone or together.

[0083] Before nucleic acid extraction, part of the magnetic rod sleeve assembly 12 is pre-stretched into the reagent tube 4 which needs to be subjected to nucleic acid extraction. Then, the second driving member 13 is controlled to move, so that the magnetic rod 14 moves downward until the magnetic rod 14 is in place. After the magnetic rod 14 is in place, part of the magnetic rod 14 is stretched into the inside of the magnetic rod sleeve assembly 12, so as to be able to pick up the magnetic beads in a reagent tube 4. After the magnetic beads are picked up, the first driving member 11 and the second driving member 13 are controlled to rotate together, so that the magnetic rod 14 and the magnetic rod sleeve assembly 12 (the magnetic rod sleeve assembly 12 adsorbs the magnetic beads, and the magnetic beads adsorb the nucleic acid) move upward at the same speed. After the magnetic rod sleeve assembly 12 is separated from the reagent tube 4, the reagent tube carrier 1 moves to make another reagent tube 4 move to the position directly below the magnetic rod sleeve assembly 12. After the reagent tube carrier 1 is in place, the first driving member 11 and the second driving member 13 are controlled to rotate together again, so that the magnetic rod 14 and the magnetic rod sleeve assembly 12 (the magnetic rod sleeve assembly 12 adsorbs the magnetic beads, and the magnetic beads adsorb the nucleic acid) move downward at the same speed, so as to make the magnetic rod sleeve assembly 12 adsorbing the magnetic beads stretch into the reagent tube 4 below. After the magnetic rod 14 and the magnetic rod sleeve assembly 12 move to the position, the second driving member 13 is controlled to rotate, so that the magnetic rod 14 moves upward and away from the magnetic rod sleeve assembly 12, so as to release the magnetic beads and wash the magnetic beads adsorbing the nucleic acid.

[0084] ​The extraction device in the embodiment has simple structure, the first driving member 11 and the second driving member 13 are spaced apart in the vertical direction, and the second driving member 13 overlaps the first driving member 11 in the width direction of the bottom plate 10, so that the width of the first driving member 11 and the second driving member 13 as a whole is consistent with the width of the first driving member 11 (or the width of the second driving member 13), which is conducive to reducing the overall width of the extraction device; since the arrangement direction of the first driving member 11 and the arrangement direction of the second driving member 13 are both along the length direction of the bottom plate 10, the width occupied by the first driving member 11 and the second driving member 13 inside the extraction device can be further reduced, so that the layout between the components of the extraction device is more compact, which is conducive to reducing the volume of the extraction device and the nucleic acid extraction equipment, facilitating the miniaturization design of the extraction device and the nucleic acid extraction equipment, and facilitating the nucleic acid extraction equipment to be directly placed in a biological safety cabinet.

[0085] In the description of the present application, it should be understood that the terms "first", "second" are used only for descriptive purposes and should not be construed as indicating or implying relative importance or implicitly indicating the number of the technical features indicated. Therefore, the features defined as "first", "second" can explicitly or implicitly include at least one of the features. In the description of the present application, the meaning of "a plurality of" is at least two, such as two, three, etc., unless otherwise specifically limited.

[0086] In the present application, unless otherwise specifically defined and limited, the terms "mounting", "connecting", "connecting", "fixing" and the like should be understood in a broad sense, for example, it can be fixedly connected, or it can be detachably connected, or it can be integrated; it can be mechanically connected, or it can be electrically connected or communicate with each other; it can be directly connected, or it can be indirectly connected through an intermediate medium; it can be the internal communication of two elements or the interaction relationship between two elements, unless otherwise specifically limited. For those skilled in the art, the specific meaning of the above terms in the present application can be understood according to the specific circumstances.

[0087] In the description of the present application, the description of the terms "one embodiment", "some embodiments", "example", "specific example", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present application. In the present description, the illustrative description of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art can combine and combine the different embodiments or examples described in the present description and the features of the different embodiments or examples without contradiction.

[0088] Although the embodiments of the present application have been shown and described above, it is understood that the above-described embodiments are exemplary and are not to be construed as limiting the present application, and that changes, modifications, substitutions and variations can be made by those skilled in the art without departing from the scope of the present application.

Claims

1. A nucleic acid extraction apparatus, characterized by, The nucleic acid extraction device comprises: a reagent tube carrier (1) having a receiving space (101) formed thereon and recessed from top to bottom and used for placing a reagent tube (4); an ultrasonic mechanism (2) arranged on a lateral side of the reagent tube carrier (1) and used for outputting ultrasonic waves to reagents in the reagent tube (4); a clamping mechanism (3) comprising a first clamping piece (301), a second clamping piece (302) and an elastic assembly, the first clamping piece (301) being arranged on the reagent tube carrier (1) and located on one side of the receiving space (101) and being laterally movable, the second clamping piece (302) being arranged on the reagent tube carrier (1) and located on the other side of the receiving space (101) and being laterally movable, the second clamping piece (302) being connected with the first clamping piece (301), and the elastic assembly being used for adjusting the width between the first clamping piece (301) and the second clamping piece (302) to clamp the reagent tube (4).

2. The nucleic acid extraction apparatus according to claim 1, wherein The clamping mechanism (3) further comprises a connecting assembly (303) arranged on the reagent tube carrier (1) and connected with the first clamping piece (301) and the second clamping piece (302) at two lateral ends thereof, respectively, and one end of the elastic assembly is abutted against the first clamping piece (301) and the other end of the elastic assembly is abutted against the reagent tube carrier (1).

3. The nucleic acid extraction apparatus according to claim 2, characterized by The reagent tube carrier (1) has a first partition (102) and a second partition (103) formed thereon and located at two longitudinal ends of the reagent tube carrier (1) and between the first clamping piece (301) and the second clamping piece (302), respectively, the connecting assembly (303) comprises a first connecting piece (3031) and a second connecting piece (3032), the first connecting piece (3031) is movably arranged on the first partition (102) and connected with the first clamping piece (301) and the second clamping piece (302) at two ends thereof, respectively, the second connecting piece (3032) is movably arranged on the second partition (103) and connected with the first clamping piece (301) and the second clamping piece (302) at two ends thereof, respectively, and the elastic assembly comprises a first elastic piece and a second elastic piece, the first elastic piece is sleeved on the outside of the first connecting piece (3031) and abutted against the first clamping piece (301) and the first partition (102) at two ends thereof, respectively, and the second elastic piece is sleeved on the outside of the second connecting piece (3032) and abutted against the first clamping piece (301) and the second partition (103) at two ends thereof, respectively.

4. The nucleic acid extraction apparatus according to claim 1, wherein The reagent tube (4) comprises a cylindrical portion (401) and an arc surface portion (402) located at the bottom end of the cylindrical portion (401), and the nucleic acid extraction device further comprises a limiting piece (5) arranged at the bottom of the receiving space (101), the limiting piece (5) has a receiving groove (501) formed thereon and recessed from top to bottom and used for accommodating part of the arc surface portion (402).

5. The nucleic acid extraction apparatus according to claim 4, wherein The limiting piece (5) comprises a base portion (502) and a stop portion (503), the accommodating groove (501) is formed on the base portion (502), and the stop portion (503) is arranged on the top of the base portion (502) and located on the side of the base portion (502) away from the ultrasonic mechanism (2).

6. The nucleic acid extraction apparatus according to claim 5, wherein A first arc-shaped wall (504) capable of abutting the outer peripheral wall of the reagent tube (4) is formed on the side of the stop portion (503) facing the reagent tube (4).

7. The nucleic acid extraction apparatus according to claim 4, wherein The limiting piece (5) is made of a heat-conducting material, and the nucleic acid extraction device further comprises a heating piece capable of emitting heat and in contact with the limiting piece (5).

8. The nucleic acid extraction apparatus according to claim 7, wherein The nucleic acid extraction device further comprises a heat insulation piece (6) embedded on the reagent tube carrier (1) and located below the limiting piece (5).

9. The nucleic acid extraction apparatus according to claim 1, wherein The ultrasonic mechanism (2) comprises a probe mounting seat (201) and an ultrasonic probe (202), the probe mounting seat (201) is arranged on the lateral side of the reagent tube carrier (1), the ultrasonic probe (202) is laterally telescopically arranged on the probe mounting seat (201), the nucleic acid extraction device further comprises a moving guide rail (7) extending in the longitudinal direction, the reagent tube carrier (1) is movably arranged on the moving guide rail (7), and the number of the reagent tubes (4) is multiple, and the multiple reagent tubes (4) are arranged on the reagent tube carrier (1) and distributed along the extension direction of the moving guide rail (7).

10. The nucleic acid extraction apparatus according to any one of claims 1 to 9, wherein The nucleic acid extraction device further comprises an extraction device for extracting and transferring nucleic acids in the reagent, and the extraction device comprises: a bottom plate (10), the bottom plate (10) is provided with mounting areas (1001) and sample areas (1002) distributed side by side in the width direction; a first driving piece (11) arranged on the mounting areas (1001) and distributed along the length direction of the bottom plate (10); a magnetic rod sleeve assembly (12) arranged above the sample areas (1002) and vertically movable, the magnetic rod sleeve assembly (12) is drivingly connected with the first driving piece (11); a second driving piece (13) arranged above the first driving piece (11), the arrangement direction of the second driving piece (13) is consistent with the length direction of the bottom plate (10), and in the width direction of the bottom plate (10), the second driving piece (13) overlaps with the first driving piece (11); a magnetic rod (14) arranged above the sample areas (1002) and vertically movable, the magnetic rod (14) is drivingly connected with the second driving piece (13) and can partially extend into the inside of the magnetic rod sleeve assembly (12) or be separated from the magnetic rod sleeve assembly (12) under the driving action of the second driving piece (13).

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