Variable-temperature synthesis box and variable-temperature synthesis device
By combining the heat-conducting and temperature-changing components of the variable-temperature synthesis chamber, and utilizing semiconductor cooling chips and temperature controllers, the temperature of the synthesis column can be precisely adjusted, solving the problem that traditional synthesizers cannot react at the optimal temperature, improving the efficiency of biochemical synthesis reactions and optimizing waste liquid discharge.
Patent Information
- Application Number
- CN202520248052.4
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-17
- Publication Date
- 2025-12-19
- Estimated Expiration
- 2035-02-17
AI Technical Summary
Traditional multi-channel column synthesizers cannot perform biochemical synthesis reactions at the optimal temperature for different reaction steps, which affects reaction efficiency.
A variable-temperature synthesis chamber is used, which combines heat conduction components and temperature control components to achieve precise temperature control of the synthesis column. The temperature is adjusted by using a semiconductor cooling chip and a temperature controller, and the heat is conducted to the synthesis column through the heat conduction components.
It enables rapid adjustment of the synthesis column temperature, improves the efficiency of biochemical synthesis reactions, and optimizes waste liquid discharge through the waste liquid chamber and drainage structure, thus expanding the function of the variable temperature synthesis chamber.
Smart Images
Figure CN223688356U_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application relates to the technical field of biochemical synthesis equipment, and particularly relates to a variable-temperature synthesis box and a variable-temperature synthesis device. BACKGROUND
[0002] In the biochemical synthesis reaction of a traditional multi-channel column type chemical synthesis instrument, the synthesis enzymes required by each reaction step may be different, and the optimal activity temperatures corresponding to different synthesis enzymes may be different. However, the traditional multi-channel column type chemical synthesis instrument can only perform biochemical synthesis reaction at a fixed environmental temperature, and cannot make the synthesis enzymes required by each reaction step be at the optimal activity temperature to perform synthesis reaction, which seriously affects the efficiency of biochemical synthesis reaction. CONTENT OF THE UTILITY MODEL
[0003] Therefore, the present application aims to provide a variable-temperature synthesis box and a variable-temperature synthesis device, which can overcome the shortcomings of the prior art.
[0004] In order to achieve the above-mentioned purpose, the technical scheme adopted by the present application is as follows:
[0005] The first embodiment of the present application discloses a variable-temperature synthesis box, which comprises a box body, a heat conduction assembly and a variable-temperature assembly.
[0006] The box body is provided with a plurality of accommodation portions.
[0007] The heat conduction assembly is arranged in the accommodation portion, and the heat conduction assembly is provided with a plurality of heat conduction cavities. The variable-temperature assembly is arranged at the bottom of the accommodation portion and located below the heat conduction assembly, and the variable-temperature assembly is connected with the heat conduction assembly.
[0008] When the synthesis column is placed in the heat conduction cavity, the variable-temperature assembly conducts heat to the synthesis column through the heat conduction assembly to change the temperature of the synthesis column.
[0009] As an implementation manner, the heat conduction assembly comprises an upper heat conduction block and a lower heat conduction block from top to bottom. The upper heat conduction block is provided with the plurality of heat conduction cavities, and the lower heat conduction block is provided with a plurality of heat conduction columns. The lower heat conduction block is connected with the upper heat conduction block through the plurality of heat conduction columns.
[0010] The variable-temperature assembly is located below the lower heat conduction block, and the variable-temperature assembly is connected with the lower heat conduction block. The variable-temperature assembly conducts heat to the upper heat conduction block through the plurality of heat conduction columns of the lower heat conduction block, so as to conduct temperature to the synthesis column through the plurality of heat conduction cavities of the upper heat conduction block.
[0011] As an implementation manner, the lower heat conduction block is further provided with a waste liquid chamber, and the plurality of heat conduction columns are located in the waste liquid chamber. The waste liquid chamber is used for accommodating the waste liquid discharged by the synthesis column.
[0012] As an implementation form, the box is provided with a waste liquid main channel, the lower heat-conducting block is provided with a plurality of waste liquid discharge ports, and the waste liquid chamber of the lower heat-conducting block is communicated with the waste liquid main channel of the box through the waste liquid discharge ports.
[0013] As an implementation form, the upper heat-conducting block is provided with a plurality of connecting holes, and the lower heat-conducting block is provided with a plurality of connecting columns; the plurality of connecting holes of the upper heat-conducting block are connected with the plurality of connecting columns of the lower heat-conducting block.
[0014] As an implementation form, the bottom of the box is provided with a heat dissipation assembly, the temperature changing assembly abuts against the heat dissipation assembly, and the lower side of the heat dissipation assembly is provided with a heat dissipation fin.
[0015] As an implementation form, the box comprises a top plate and a heat insulation plate from top to bottom; the top plate is connected with the heat dissipation assembly through the heat insulation plate, and the heat insulation plate is arranged around the heat-conducting assembly.
[0016] The second embodiment of the present application discloses a temperature changing synthesis device, which comprises a temperature changing synthesis box and a plurality of synthesis columns, the plurality of synthesis columns are placed in a plurality of heat-conducting cavities of the heat-conducting assembly, and the temperature changing assembly conducts heat to the synthesis columns through the heat-conducting assembly to change the temperature of the synthesis columns.
[0017] As an implementation form, the tray is provided with an opening corresponding to each of the heat-conducting cavities; the plurality of synthesis columns are respectively connected with the corresponding openings in a clamping mode, and the plurality of synthesis columns are moved into the accommodating part of the box together with the tray, so that the plurality of synthesis columns connected with the plurality of openings in a clamping mode are correspondingly placed in the heat-conducting cavities.
[0018] As an implementation form, the accommodating part of the box is provided with a support seat; the support seat is used for supporting the tray to isolate the tray and the heat-conducting assembly.
[0019] Compared with the prior art, the present application has the following beneficial effects:
[0020] The box of the present application can place a plurality of synthesis columns in a plurality of heat-conducting cavities of the heat-conducting assembly arranged in the accommodating part, and then the temperature changing assembly conducts heat to the heat-conducting cavities of the heat-conducting assembly to conduct heat to the synthesis columns through the heat-conducting cavities to change the temperature of the synthesis columns, so that the temperature of the synthesis columns can be efficiently and quickly changed, the user can quickly adjust the temperature of the synthesis columns to the optimal activity temperature corresponding to the synthesis enzyme of the current step reaction, and the efficiency of the biochemical synthesis reaction can be improved. Moreover, the present application can discharge waste liquid in the synthesis process through the structural relationship of the waste liquid chamber, the waste liquid discharge port and the waste liquid main channel, thereby expanding the function of the temperature changing synthesis box used in the biochemical synthesis reaction.
[0021] For better understanding and implementation, the present application is described in detail below in conjunction with the accompanying drawings. BRIEF DESCRIPTION OF DRAWINGS
[0022] Figure 1 A schematic diagram of a temperature-variable synthesis box according to an embodiment of the present application;
[0023] Figure 2 A schematic diagram of a heat-conducting assembly according to an embodiment of the present application;
[0024] Figure 3 A schematic diagram of a lower heat-conducting block according to an embodiment of the present application;
[0025] Figure 4 A schematic diagram of a temperature-variable synthesis device according to an embodiment of the present application.
[0026] 1, top plate; 2, heat insulation plate; 3, upper heat-conducting block; 3.1, heat-conducting cavity; 3.2, connecting hole; 4, lower heat-conducting block; 4.1, heat-conducting column; 4.2, waste liquid chamber; 4.3, liquid discharge port; 5, temperature-variable assembly; 6, heat dissipation assembly; 7, O-ring; 8, sealing gasket; 9, tray; 10, synthesis column. DETAILED DESCRIPTION
[0027] To further illustrate the embodiments, the present application provides accompanying drawings. These drawings are part of the disclosure of the present application, which mainly serve to illustrate the embodiments, and can be interpreted in conjunction with the relevant description of the specification to understand the operating principle of the embodiments. With reference to these contents, those skilled in the art should understand other possible implementations and advantages of the present application.
[0028] In the description of the present application, it should be understood that the orientation or positional relationship indicated by the terms "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "left", "right", "top", "bottom", "inner", "outer", "axial", "radial", "circumferential" and the like are based on the orientation or positional relationship shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, and therefore cannot be understood as indicating or implying that the devices or elements referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.
[0029] Please refer to Figure 1 which is a schematic diagram of a temperature-variable synthesis box according to an embodiment of the present application, and the first embodiment of the present application provides a temperature-variable synthesis box, which comprises a box body, a heat-conducting assembly and a temperature-variable assembly 5;
[0030] The box body is provided with a plurality of accommodating portions;
[0031] The heat-conducting assembly is arranged in the accommodating portion, and is provided with a plurality of heat-conducting cavities 3.1; the temperature-variable assembly 5 is arranged at the bottom of the accommodating portion and below the heat-conducting assembly, and is connected with the heat-conducting assembly.
[0032] When the synthesis column is placed in the heat-conducting cavities 3.1, the temperature-variable assembly 5 conducts heat to the synthesis column through the heat-conducting assembly to change the temperature of the synthesis column.
[0033] The temperature-variable assembly 5 can adopt a semiconductor refrigeration sheet, which is a heat source of a temperature control system and can control the current passing therethrough through a program to control the semiconductor refrigeration sheet to heat or cool, and then conduct heat to the synthesis column through the heat-conducting assembly to heat or cool the synthesis column.
[0034] In some biochemical synthesis reaction processes, steps such as deprotection, activation linkage, washing, and mixing are continuously cycled, so the temperature needs to be adjusted in real time according to the synthesis steps during the entire biochemical synthesis reaction process. For the temperature-variable synthesis box, when heating, a forward current is supplied to the semiconductor refrigeration sheet to start heating the upper surface of the semiconductor refrigeration sheet, and then the heat is conducted to the synthesis column through the heat-conducting assembly to increase the temperature of the synthesis column; when cooling, a reverse current is supplied to the semiconductor refrigeration sheet to start cooling the upper surface of the semiconductor refrigeration sheet, and then the heat is conducted to the synthesis column through the heat-conducting assembly to decrease the temperature of the synthesis column. The temperature controller can control the current size and direction output to the semiconductor refrigeration sheet, for example, the temperature controller can control the current size and direction output to the semiconductor refrigeration sheet through a PID algorithm, and the temperature of the heat-conducting cavities 3.1 or the synthesis column can be detected by a temperature sensor to achieve accurate temperature control.
[0035] Please refer to Figure 2 In a feasible embodiment, the heat-conducting assembly comprises an upper heat-conducting block 3 and a lower heat-conducting block 4 from top to bottom; the upper heat-conducting block 3 is provided with the plurality of heat-conducting cavities 3.1, and the lower heat-conducting block 4 is provided with a plurality of heat-conducting columns 4.1, and the lower heat-conducting block 4 is connected with the upper heat-conducting block 3 through the plurality of heat-conducting columns 4.1.
[0036] The temperature-variable assembly 5 is located below the lower heat-conducting block 4, is connected with the lower heat-conducting block 4, and conducts heat to the upper heat-conducting block 3 through the plurality of heat-conducting columns 4.1 of the lower heat-conducting block 4 to conduct temperature to the synthesis column through the plurality of heat-conducting cavities 3.1 of the upper heat-conducting block 3.
[0037] Please refer to Figure 3In an embodiment, the lower heat-conducting block 4 is further provided with a waste liquid chamber 4.2, and the plurality of heat-conducting columns 4.1 are located in the waste liquid chamber 4.2; the waste liquid chamber 4.2 is used to accommodate the waste liquid discharged from the synthesis column.
[0038] In an embodiment, the box is provided with a main waste liquid passage, the lower heat-conducting block 4 is provided with a plurality of waste liquid discharge ports 4.3, and the waste liquid chamber 4.2 of the lower heat-conducting block 4 is in communication with the main waste liquid passage of the box through the waste liquid discharge ports 4.3.
[0039] After each step in the biochemical synthesis reaction process is completed, the reagent of the synthesis column after the completion of the reaction needs to be discharged into the waste liquid barrel. Specifically, the process can be implemented as follows:
[0040] The liquid in the synthesis column flows downward into the waste liquid chamber 4.2 in the lower heating block under the action of the liquid discharge pressure; the bottom surface of the waste liquid chamber 4.2 is a certain distance away from the bottom surface of the synthesis column, and this part of space is used to temporarily accommodate the waste liquid discharged from the synthesis column; the waste liquid flowing into the waste liquid chamber 4.2 is discharged into the main waste liquid passage through the waste liquid discharge ports 4.3 under the action of the liquid discharge pressure, and finally flows into the waste liquid barrel.
[0041] Preferably, the waste liquid discharge ports 4.3 are uniformly arranged around the waste liquid chamber 4.2, which can make the waste liquid chamber 4.2 uniformly discharge waste liquid through the waste liquid discharge ports 4.3, prevent slow waste liquid discharge at some positions and cause waste liquid backflow, and prevent the synthesis column from being contaminated due to waste liquid backflow.
[0042] In an embodiment, the upper heat-conducting block 3 is provided with a plurality of connecting holes 3.2, and the lower heat-conducting block 4 is provided with a plurality of connecting columns; the plurality of connecting holes 3.2 of the upper heat-conducting block 3 are connected with the plurality of connecting columns of the lower heat-conducting block 4.
[0043] Each connecting column is provided with an internal screw hole, a screw can pass through the connecting hole 3.2 and enter the internal screw hole of the connecting column, the external thread of the screw is connected with the internal thread of the internal screw hole, the screw is connected with the internal screw hole of the connecting column, and the upper heat-conducting block 3 is attached to and fixed on the upper surface of the lower heat-conducting block 4.
[0044] In an embodiment, the bottom of the box is provided with a heat dissipation assembly 6, the temperature changing assembly 5 abuts against the heat dissipation assembly 6, and the heat dissipation assembly 6 is provided with heat dissipation fins below.
[0045] In an embodiment, the box comprises a top plate 1 and a heat insulation plate 2 from top to bottom; the top plate 1 is connected with the heat dissipation assembly 6 through the heat insulation plate 2, and the heat insulation plate 2 is arranged around the heat-conducting assembly.
[0046] Compared with the prior art, the application has the following advantages:
[0047] The box of the present application can place multiple synthetic columns through multiple heat conduction cavities 3.1 of the heat conduction assembly arranged in the accommodation part, and then the temperature changing assembly 5 conducts heat to the heat conduction cavities 3.1 of the heat conduction assembly, so as to conduct heat to the synthetic columns through the heat conduction cavities 3.1, so as to change the temperature of the synthetic columns. The temperature of the synthetic columns can be efficiently and quickly changed, the user can quickly adjust the temperature of the synthetic columns to the temperature corresponding to the optimal activity of the synthetic enzyme of the current step reaction, and the efficiency of the biochemical synthesis reaction can be improved. Moreover, the present application can also discharge waste liquid in the synthesis process through the structural relationship of the waste liquid chamber 4.2, the liquid discharge port 4.3 and the waste liquid main channel, thereby expanding the function of the temperature changing synthesis box when used for biochemical synthesis reaction.
[0048] Please continue to refer to Figure 1 The temperature changing synthesis box of the present application can also be provided with an O-ring 7 and a sealing gasket 8 for improving the sealing performance of each structural component of the temperature changing synthesis box. The sealing gasket 8 can be replaced by an O-ring 7 or an air-tight sealing ring.
[0049] Please refer to Figure 4 The second embodiment of the present application discloses a temperature changing synthesis device, which comprises a temperature changing synthesis box and multiple synthetic columns 10. The multiple synthetic columns 10 are placed in multiple heat conduction cavities 3.1 of the heat conduction assembly. The temperature changing assembly 5 conducts heat to the synthetic columns 10 through the heat conduction assembly, so as to change the temperature of the synthetic columns 10.
[0050] As an embodiment, the box further comprises a tray 9 provided with a plurality of openings corresponding to the heat conduction cavities 3.1. The multiple synthetic columns 10 are respectively connected with the corresponding openings by clamping. The multiple synthetic columns 10 are moved into the accommodation part of the box together with the tray 9, so that the multiple synthetic columns 10 connected with the multiple openings are correspondingly moved into the heat conduction cavities 3.1.
[0051] The tray 9 can be a tray 9 provided with 96 openings, or a tray 9 provided with 24, 48 or other numbers of openings.
[0052] As an embodiment, the accommodation part of the box is provided with a support seat. The support seat is used to support the tray 9, so as to isolate the tray 9 and the heat conduction assembly.
[0053] Compared with the prior art, the present application has the following advantages:
[0054] The box of the present application can place a plurality of synthetic columns 10 through a plurality of heat conduction cavities 3.1 of the heat conduction assembly arranged in the accommodation portion, and then the temperature changing assembly 5 conducts heat to the heat conduction cavities 3.1 of the heat conduction assembly to conduct heat to the synthetic columns 10 through the heat conduction cavities 3.1 to change the temperature of the synthetic columns 10, which can efficiently and quickly change the problem of the synthetic columns 10, facilitate the user to quickly adjust the temperature of the synthetic columns 10 to the temperature corresponding to the optimal activity of the synthetic enzyme of the current step reaction, and improve the efficiency of the biochemical synthesis reaction. Moreover, the present application can also discharge waste liquid in the synthesis process through the structural relationship of the waste liquid chamber 4.2, the liquid discharge port 4.3 and the waste liquid main channel, thereby expanding the function of the temperature changing synthesis box when used for biochemical synthesis reaction.
[0055] The above-described embodiments only express several embodiments of the present application, which are described in detail and specifically, but should not be understood as limiting the protection scope of the present application. It should be pointed out that, for ordinary skilled persons in the art, several modifications and improvements can be made without departing from the concept of the present application, which all belong to the protection scope of the present application.
Claims
1. A temperature swing synthesis box, characterized in that: The application relates to a temperature-variable synthesis box. The box body is provided with a plurality of accommodating portions. The heat-conducting assembly is arranged in the accommodating portion, and the heat-conducting assembly is provided with a plurality of heat-conducting cavities. The temperature-variable assembly is arranged at the bottom of the accommodating portion and below the heat-conducting assembly, and the temperature-variable assembly is connected with the heat-conducting assembly. When the synthesis column is placed in the heat-conducting cavity, the temperature-variable assembly conducts heat to the synthesis column through the heat-conducting assembly to change the temperature of the synthesis column. The heat-conducting assembly comprises an upper heat-conducting block and a lower heat-conducting block from top to bottom.
2. The thermocycling synthesis block of claim 1, wherein: The upper heat-conducting block is provided with the plurality of heat-conducting cavities, and the lower heat-conducting block is provided with a plurality of heat-conducting columns. The temperature-variable assembly is arranged below the lower heat-conducting block and is connected with the lower heat-conducting block.
3. The thermocycling block of claim 2, wherein: The temperature-variable assembly conducts heat to the upper heat-conducting block through the plurality of heat-conducting columns of the lower heat-conducting block, and then conducts the temperature to the synthesis column through the plurality of heat-conducting cavities of the upper heat-conducting block.
4. The thermocycling block of claim 3, wherein: The lower heat-conducting block is further provided with a waste liquid chamber, and the plurality of heat-conducting columns are arranged in the waste liquid chamber.
5. The thermocycling synthesis block of claim 2, wherein: The waste liquid chamber is used for containing waste liquid discharged by the synthesis column.
6. The thermocycling synthesis block of claim 1, wherein: The box body is provided with a waste liquid main passage, and the lower heat-conducting block is provided with a plurality of liquid discharge ports.
7. The thermocycling block of claim 6, wherein: The upper heat-conducting block is provided with a plurality of connecting holes, and the lower heat-conducting block is provided with a plurality of connecting columns.
8. A temperature swing synthesis apparatus, characterized by: The bottom of the box body is provided with a heat-dissipating assembly.
9. The thermostat synthesis device of claim 8, wherein: The box body comprises a top plate and a heat-insulating plate from top to bottom.
10. The thermostat synthesis apparatus of claim 9, wherein: The top plate is connected with the heat-dissipating assembly through the heat-insulating plate, and the heat-insulating plate is arranged around the heat-conducting assembly. The application further relates to a tray provided with a plurality of openings corresponding to the heat-conducting cavities. The plurality of synthesis columns are respectively connected with the corresponding openings in a clamping mode, and the plurality of synthesis columns are moved into the accommodating portion of the box body along with the tray. The accommodating portion of the box body is provided with a supporting seat. The supporting seat is used for supporting the tray to isolate the tray and the heat-conducting assembly.