Device for blood sorting of central memory T cells

Abstract

The utility model relates to the technical field of blood sorting, in particular to a device for blood sorting of central memory T cells, which comprises a sorting machine, a sample bin arranged in the sorting machine, an adjusting component arranged in the sample bin and used for detecting and controlling the flow velocity of a sample, and a conveying groove arranged at one end of the sample bin, an exciting light device and a pressurizing bin are arranged on the inner wall of the conveying groove; an annular valve and a plurality of baffles are arranged in the conveying groove, abutting blocks are arranged at one ends of the baffles, connectors and connecting rods are arranged on the outer sides of the baffles, pistons are arranged at the other ends of the connecting rods, and a plurality of air bins are arranged on the inner wall of the conveying groove. According to the utility model, the pressure during sample flowing is monitored, the flow rate of the sample is controlled and adjusted, the stability of the flow rate of the sample is improved, and the quality and efficiency of sorting treatment are improved.

Description

Technical Field

[0001] This utility model relates to the field of blood sorting technology, specifically to a device for sorting central memory T cells in blood. Background Technology

[0002] Central memory T cells (TCMs) are a subset of T cells that play a crucial role in the immune response. TCMs can home to lymph nodes and respond rapidly upon re-encountering the same antigen, providing fast and effective immune protection. TCMs in blood can be sorted using flow cytometry, a technique that uses flow cytometry to rapidly and quantitatively analyze the physicochemical properties of cell subsets. TCMs can be precisely isolated from blood samples using methods such as fluorescence-activated cell sorting or magnetic activation cell sorting.

[0003] However, in current flow cytometry for cell sorting, the varying concentrations of blood samples lead to differences in flow rate. Higher flow rates result in coarser fluid flow at the sample center, poorer laser focusing, and lower resolution. This means that at high flow rates, the ability to distinguish cell subpopulations decreases, potentially reducing sorting accuracy and consequently affecting sorting efficiency and yield. Utility Model Content

[0004] The purpose of this invention is to provide a device for sorting central memory T cells in blood, so as to solve the problems mentioned in the background art.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A device for sorting central memory T cells in blood includes a sorting machine, the sorting machine having a sample chamber inside, the sample chamber having an adjustment component for detecting and controlling the flow rate of the sample, the adjustment component including a conveying trough disposed at one end of the sample chamber, the inner wall of the conveying trough being provided with an exciter and a pressurization chamber.

[0007] The conveying trough is equipped with an annular valve and multiple baffles. Each baffle has an abutment block at one end, a connector and a connecting rod on the outside of the baffle, and a piston at the other end of the connecting rod. The inner wall of the conveying trough is equipped with multiple air chambers.

[0008] As a preferred embodiment of this utility model, one end of the sample chamber is a conical structure and communicates with the conveying groove. The exciter is embedded and connected to the inner wall of the conveying groove, and the annular valve is located at the end of the conveying groove near the sample chamber.

[0009] As the preferred scheme of the utility model, multiple baffles and air warehouses are annularly distributed on the inner wall of the conveying groove, and the positions of the baffle and the air warehouse correspond, multiple baffles are conical structures, and are all rotationally connected with the inner wall of the conveying groove through spring shafts.

[0010] As the preferred scheme of the utility model, the connecting head is located on the outer wall of the baffle and is in sliding connection with the outer wall of the baffle, one end of the connecting rod is rotationally connected with the connecting head through a fixed shaft, and the other end extends into the air warehouse.

[0011] As the preferred scheme of the utility model, the connecting rod is connected with the piston at one end in the air warehouse through bolts, the piston is in sliding connection with the inside of the air warehouse, the inside of the air warehouse is loaded with high-pressure gas, and is provided with a gas pressure sensor.

[0012] As the preferred scheme of the utility model, the output end of the pressurizing warehouse is provided with a one-way valve, and the gas pressure sensor in the air warehouse is electrically connected with the one-way valve of the pressurizing warehouse.

[0013] Compared with the prior art, the utility model has the beneficial effects that: aiming at the problems in the background art, the application adopts an adjusting assembly, the aperture of the sample conveying groove can be adjusted through the annular valve in the conveying groove, so that the conveying speed is adjusted, when the sample passes through the annular valve, the sample abuts against the baffle in the conveying groove, is extruded between the annular valve and the baffle, is pushed away by the extruded baffle and is output, the pressure during output corresponds to the strength of the baffle, when the baffle is unfolded, the air warehouse is extruded through the connecting rod, the efficiency of sample conveying can be monitored by cooperating with the pressure change in the air warehouse, the output speed of the sample is controlled and adjusted by controlling the annular valve and the pressurizing warehouse switch, the efficiency and flow rate of sample conveying are adjusted and controlled, the stability of sample conveying is improved, and the sorting stability and quality are increased.

[0014] The utility model realizes the pressure monitoring of sample flow and the control and adjustment of sample flow rate, improves the stability of sample flow rate, and increases the quality and efficiency of sorting processing. BRIEF DESCRIPTION OF DRAWINGS

[0015] Fig. 1 It is the appearance structure diagram of the sorting machine of the utility model.

[0016] Fig. 2 It is the sample conveying warehouse sectional view of the utility model.

[0017] Fig. 3 It is the A part enlarged view of the utility model.

[0018] In the drawing: 1, sorting machine;2, sample warehouse;3, conveying groove;301, excitation light device;302, annular valve;4, pressurizing warehouse;5, baffle;501, abutting block;6, connecting head;7, connecting rod;701, piston;8, air warehouse. DETAILED DESCRIPTION

[0019] The technical scheme in the embodiments of the utility model will be clearly and completely described in connection with the embodiments of the utility model. EMBODIMENT

[0020] Please refer to Figs. 1-3 The utility model provides a technical scheme: a device for blood sorting central memory T cell, including sorting machine 1, the inside of sorting machine 1 is provided with sample bin 2, for the temporary storage to sample, and transfer, the inside of sample bin 2 is provided with the adjusting assembly for the flow rate detection and control of sample, the adjusting assembly includes the conveying groove 3 of setting in sample bin 2 one end, for conveying sample, the inner wall of conveying groove 3 is provided with excitation light ware 301 and pressurized bin 4;Excitation light ware 301 can emit light, and flow cytometer carries out cell analysis and sorting by measuring the biophysical and biochemical characteristic parameters of cell.When cell passes through the measuring area, laser beam irradiates to single cell, and cell will scatter light, and the intensity and spatial distribution of this scattered light are related to the size, morphology etc.of cell.At the same time, laser excitation excites all fluorophores related to cell, produces fluorescence emission, and these fluorescence signals are collected by detector and are handled through electronic element.

[0021] The inside of conveying groove 3 is provided with annular valve 302 and multiple baffles 5, and annular valve 302 can adjust the aperture of conveying groove 3, and the flow of sample conveying can be adjusted by the size of aperture, and sample is abutted with the baffle 5 of the inner wall of conveying groove 3 after passing through annular valve 302 and is extruded between annular valve 302 and baffle 5, to increase the pressure of sample, and baffle 5 is opened by pressure and transports, and the greater the pressure of sample, the greater the amplitude of baffle 5 opening and the faster the flow rate, and baffle 5 is opened to drive connecting rod 7 to contract in air bin 8, to increase the pressure in air bin 8, the pressure change in air bin 8 is detected by pressure sensor, the flow rate of sample is detected by pressure change, and the flow rate of sample is controlled by annular valve 302 or pressurized bin 4 (the flow of sample is reduced to reduce the flow rate by annular valve 302, and the pressure of sample is increased to increase the flow rate by pressurized bin 4), and one end of multiple baffles 5 is provided with abutting block 501, the outer side of baffle 5 is provided with connecting head 6 and connecting rod 7, the other end of connecting rod 7 is provided with piston 701, and the inner wall of conveying groove 3 is provided with multiple air bins 8.

[0022] All electrical elements in the embodiment are controlled by conventional controller.

[0023] EMBODIMENT, please refer to Figs. 1-3The utility model discloses a sample flow rate control device, including sample bin 2, conveying groove 3, excitation light ware 301, ring valve 302, baffle 5, gas bin 8, pressurizing bin 4 and connecting rod 7, sample bin 2 one end is taper structure with conveying groove 3 is passed through, excitation light ware 301 is inlaid connection with conveying groove 3 inner wall, ring valve 302 is located in conveying groove 3 near sample bin 2 one end, a plurality of baffle 5 and gas bin 8 are annular distribution in conveying groove 3 inner wall, and baffle 5 and gas bin 8 position correspond, a plurality of baffle 5 are taper structure, and all are through spring shaft and conveying groove 3 inner wall rotation connection, connecting head 6 is located baffle 5 outer wall, and with baffle 5 outer wall sliding connection, connecting rod 7 one end is through fixed shaft and connecting head 6 rotation connection, the other end extends to gas bin 8 in, connecting rod 7 is located gas bin 8 in one end through bolt and piston 701 connects, piston 701 and gas bin 8 inside sliding connection, gas bin 8 inside is loaded with high pressure gas, and is equipped with gas pressure sensor, pressurizing bin 4 output is provided with check valve, gas pressure sensor in gas bin 8 and the check valve electric connection of pressurizing bin 4.In use, first sample is injected into sample bin 2 and flows and flows into conveying groove 3, simultaneously through PLC controller control ring valve 302 switch adjustment conveying groove 3's aperture switch, make sample enter conveying groove 3 and transport, and with conveying groove 3's baffle 5 abuts and through the pressure of accumulation and pushes baffle 5 and continues to transport when, when baffle 5 opens and drives connecting rod 7 in gas bin 8 and contracts and increases the pressure in gas bin 8, simultaneously through pressure sensor and detects the pressure change, thereby sends out the signal control ring valve 302 or pressurizing bin 4 switch adjustment, thereby controls sample's flow rate and makes flow rate be in the set range.

[0024] The utility model discloses a sample flow rate control device, including sample bin 2, conveying groove 3, excitation light ware 301, ring valve 302, baffle 5, gas bin 8, pressurizing bin 4 and connecting rod 7, sample bin 2 one end is taper structure with conveying groove 3 is passed through, excitation light ware 301 is inlaid connection with conveying groove 3 inner wall, ring valve 302 is located in conveying groove 3 near sample bin 2 one end, a plurality of baffle 5 and gas bin 8 are annular distribution in conveying groove 3 inner wall, and baffle 5 and gas bin 8 position correspond, a plurality of baffle 5 are taper structure, and all are through spring shaft and conveying groove 3 inner wall rotation connection, connecting head 6 is located baffle 5 outer wall, and with baffle 5 outer wall sliding connection, connecting rod 7 one end is through fixed shaft and connecting head 6 rotation connection, the other end extends to gas bin 8 in, connecting rod 7 is located gas bin 8 in one end through bolt and piston 701 connects, piston 701 and gas bin 8 inside sliding connection, gas bin 8 inside is loaded with high pressure gas, and is equipped with gas pressure sensor, pressurizing bin 4 output is provided with check valve, gas pressure sensor in gas bin 8 and the check valve electric connection of pressurizing bin 4.In use, first sample is injected into sample bin 2 and flows and flows into conveying groove 3, simultaneously through PLC controller control ring valve 302 switch adjustment conveying groove 3's aperture switch, make sample enter conveying groove 3 and transport, and with conveying groove 3's baffle 5 abuts and through the pressure of accumulation and pushes baffle 5 and continues to transport when, when baffle 5 opens and drives connecting rod 7 in gas bin 8 and contracts and increases the pressure in gas bin 8, simultaneously through pressure sensor and detects the pressure change, thereby sends out the signal control ring valve 302 or pressurizing bin 4 switch adjustment, thereby controls sample's flow rate and makes flow rate be in the set range.

[0025] Although the embodiments of the utility model have been shown and described, it is to be understood that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the utility model, the scope of the utility model is defined by the appended claims and their equivalents.

Claims

1. A device for sorting central memory T cells from blood, comprising a sorting machine (1), wherein a sample bin (2) is arranged inside the sorting machine (1), and an adjusting assembly for detecting and controlling the flow rate of the sample is arranged inside the sample bin (2), characterized in that: The adjusting assembly comprises a conveying groove (3) arranged at one end of the sample bin (2), and an excitation light device (301) and a pressurizing bin (4) are arranged on the inner wall of the conveying groove (3); The conveying groove (3) is internally provided with an annular valve (302) and a plurality of baffles (5), one end of each of the plurality of baffles (5) is provided with an abutting block (501), the outer side of the baffle (5) is provided with a connecting head (6) and a connecting rod (7), the other end of the connecting rod (7) is provided with a piston (701), and the inner wall of the conveying groove (3) is provided with a plurality of gas bins (8).

2. The apparatus for sorting central memory T cells from blood according to claim 1, wherein: One end of the sample bin (2) is a conical structure and communicates with the conveying groove (3), the excitation light device (301) is inlaidly connected with the inner wall of the conveying groove (3), and the annular valve (302) is located at one end of the conveying groove (3) close to the sample bin (2).

3. The apparatus for sorting central memory T cells from blood according to claim 1, wherein: The plurality of baffles (5) and gas bins (8) are annularly distributed on the inner wall of the conveying groove (3), and the positions of the baffle (5) and the gas bin (8) correspond, the plurality of baffles (5) are conical structures, and are rotatably connected with the inner wall of the conveying groove (3) through spring shafts.

4. The apparatus for sorting central memory T cells from blood according to claim 1, wherein: The connecting head (6) is located on the outer wall of the baffle (5) and is slidably connected with the outer wall of the baffle (5), one end of the connecting rod (7) is rotatably connected with the connecting head (6) through a fixed shaft, and the other end extends into the gas bin (8).

5. The apparatus for sorting central memory T cells from blood according to claim 1, wherein: One end of the connecting rod (7) in the gas bin (8) is connected with the piston (701) through a bolt, the piston (701) is slidably connected with the inner part of the gas bin (8), the inner part of the gas bin (8) is loaded with high-pressure gas, and a gas pressure sensor is installed.

6. The apparatus for sorting central memory T cells from blood according to claim 5, wherein: The output end of the pressurizing bin (4) is provided with a one-way valve, and the gas pressure sensor in the gas bin (8) is electrically connected with the one-way valve of the pressurizing bin (4).

Images