NK cell amplification kit

Abstract

The utility model discloses an NK (Natural Killer) cell amplification kit, which relates to the technical field of cell amplification, and comprises a reagent storage box, a box cover, an ultraviolet sterilization lamp tube, a vent hole, a condensate feeding pipe and a control valve, an operator holds a handle, pushes upwards, lifts the box cover and puts a reagent bottle into a reagent clamping seat, and when the reagent bottle is put into the reagent clamping seat, the ultraviolet sterilization lamp tube is inserted into the vent hole. The spring rods are stressed to extrude the springs inwards, so that the semi-arc rubber clamping plates can be in contact with the reagent bottles, the springs rebound, the spring rods push the semi-arc rubber clamping plates to clamp the reagent bottles, and rubber pads are mounted between the semi-arc rubber clamping plates and the reagent bottles at intervals, so that the reagent bottles can be well prevented from being extruded to be damaged; the reagent dropper is used for dropping or sucking a reagent from the reagent bottle, when the rubber head of the reagent dropper is squeezed, air in the dropper is exhausted, then the dropper is inserted into the reagent bottle, the rubber head is loosened, and the reagent can be sucked into the dropper due to the fact that the pressure in the dropper is lower than that in the reagent bottle.

Description

Technical Field

[0001] This invention relates to the field of cell amplification technology, specifically an NK cell amplification kit. Background Technology

[0002] Natural killer (NK) cells are important immune cells in the body's immune system, possessing functions such as anti-tumor, anti-infection, and immune regulation. However, the relatively low concentration of NK cells in human peripheral blood limits their widespread application in immunotherapy, basic research, and other fields. To obtain a sufficient number of NK cells, effective NK cell expansion technologies need to be developed. However, existing NK cell expansion kits may suffer from breakage due to shaking during transportation, resulting in a high breakage rate. Therefore, those skilled in the art have provided an NK cell expansion kit to address the problems mentioned in the background section. Utility Model Content

[0003] The purpose of this invention is to provide an NK cell amplification kit to solve the problems mentioned in the background art.

[0004] To achieve the above objectives, this utility model provides the following technical solution:

[0005] An NK cell amplification kit includes a reagent storage box, a lid, an ultraviolet germicidal lamp, a ventilation hole, a condensate feeding tube, and a control valve. The lid is connected to the top of the reagent storage box and has a handle fixedly connected to the front end of the lid. A first retaining plate is movably connected to the lower part of the inside of the reagent storage box. Several reagent holders are fixedly connected to the top of the first retaining plate. Clamping components are fixedly connected to the left and right sides of the inside of the reagent holders. A spring is fixedly connected to the inner wall surface of the clamping component. A spring rod is fixedly connected to the other end of the spring. A semi-circular rubber clamping plate is fixedly connected to the other end of the spring rod. A reagent bottle is movably connected to the inner wall surface of the semi-circular rubber clamping plate. A second support is movably connected to the upper part of the inner wall surface of the reagent storage box. A reagent dropper is movably connected to the top of the second support.

[0006] As a further embodiment of this utility model: ceramic heads are movably connected to the top left and right sides of the box cover, and ultraviolet germicidal lamp tubes are movably connected to the inner wall surface of the ceramic heads.

[0007] As a further improvement of this utility model: several ventilation holes are provided on the lower left and right sides of the reagent storage box, and a condensate feeding pipe is fixedly connected to the lower left side of the reagent storage box.

[0008] As a further embodiment of this utility model: the end of the condensate feeding pipe is fixedly connected to a circulation pipe, and the lower left side of the reagent storage box is movably connected to a circulation pump.

[0009] As a further improvement of this utility model: the top of the first card plate is provided with several cold air vents, and the bottom right end of the circulation pipe is fixedly connected to the condensate outlet pipe.

[0010] As a further embodiment of this utility model: the outer wall surface of the condensate outlet tube is movably connected to the control valve at the bottom right end of the reagent storage box, and the outer wall surface of the control valve is movably connected to the pressure valve at the chisel.

[0011] As a further improvement of this utility model: the left and right ends of the reagent storage box are provided with several sealing elements, and the inner surface of the sealing elements is fixedly connected to the inner wall surface of the ventilation hole with a ventilation sealing ring.

[0012] As a further improvement of this utility model: a function display screen is provided on the right side of the front end of the reagent storage box, and several function knobs are provided on the lower right side of the front end of the reagent storage box.

[0013] Compared with the prior art, the beneficial effects of this utility model are:

[0014] 1. The operator holds the handle and pushes upwards to lift the lid. The operator places the reagent bottle into the reagent holder. Upon insertion, the spring rod compresses the spring inwards, allowing the semi-circular rubber clamping plate to contact the reagent bottle. The spring returns, causing the spring rod to push the semi-circular rubber clamping plate to hold the reagent bottle. Rubber pads are installed between the semi-circular rubber clamping plate and the reagent bottle to effectively prevent damage from compression. The reagent dropper is used to add or draw reagents from the reagent bottle. When the rubber bulb of the dropper is squeezed, the air inside the dropper is expelled. Then, the dropper is inserted into the reagent bottle. Releasing the rubber bulb causes the reagent to be drawn into the dropper because the pressure inside the dropper is lower than the pressure inside the reagent bottle. When adding reagents to other containers or performing experimental operations, squeezing the rubber bulb allows the reagent to be drawn from the dropper. The reagent droppers are dispensed in the same quantity as the reagent bottles and are used one-to-one to avoid confusion. The second support is used to place the reagent dropper corresponding to the position of the reagent bottle. The elastic clamping structure, composed of clamping parts, springs, spring rods, and semi-circular clamping plates, can accommodate reagent bottles of different diameters. This allows the NK cell amplification kit to accommodate reagent bottles of various sizes, improving the kit's versatility. Under different experimental needs or reagent supply conditions, there is no need to replace the entire kit or reagent holder, saving costs and improving the kit's adaptability. The combination of reagent bottles and droppers makes the operation of aspirating and transferring reagents simple and precise. In NK cell amplification experiments, accurate reagent addition is crucial for cell growth and expansion. Attached Figure Description

[0015] Figure 1 This is a schematic diagram of the structure of an NK cell amplification kit.

[0016] Figure 2 This is a schematic diagram of the interior of the reagent storage box in an NK cell amplification kit.

[0017] Figure 3 This is a schematic diagram of the reagent slot in an NK cell amplification kit.

[0018] Figure 4 This is a magnified structural diagram of point A in an NK cell amplification kit.

[0019] In the diagram: 1-Reagent storage box, 2-Lid, 3-Handle, 4-First clamping plate, 5-Reagent holder, 6-Clamping component, 7-Spring, 8-Spring rod, 9-Semi-circular rubber clamping plate, 10-Reagent bottle, 11-Second bracket, 12-Reagent dropper, 13-Ceramic head, 14-UV germicidal lamp tube, 15-Ventilation hole, 16-Condensate feeding pipe, 17-Circulation pump, 18-Circulation pipe, 19-Cold air vent, 20-Condensate outlet pipe, 21-Control valve, 22-Press valve, 23-Sealing component, 24-Ventilation vent sealing ring, 25-Function display screen, 26-Function knob. Detailed Implementation

[0020] Please see Figures 1-4In this embodiment of the present invention, an NK cell amplification kit includes a reagent storage box 1, a box cover 2, a handle 3, a first retaining plate 4, a reagent holder 5, a clamping component 6, a spring 7, a spring rod 8, a semi-circular rubber clamping plate 9, a reagent bottle 10, a second support 11, a reagent dropper 12, a ceramic head 13, an ultraviolet germicidal lamp tube 14, a ventilation hole 15, a condensate feeding tube 16, a circulation pump 17, a circulation pipe 18, a cold air vent 19, a condensate outlet tube 20, a control valve 21, a pressure valve 22, a sealing component 23, a ventilation vent sealing ring 24, a function display screen 25, and a function knob 26. The reagent storage box 1 has a top opening and closing lid 2. A handle 3 is horizontally fixedly connected to the front end of the lid 2. A first retaining plate 4 is movably connected to the lower part of the interior of the reagent storage box 1. Several reagent holders 5 are fixedly connected to the top of the first retaining plate 4. Clamping components 6 are fixedly connected to the left and right sides of the interior of each reagent holder 5. Springs 7 are fixedly connected to the inner wall surface of the clamping components 6. A spring rod 8 is fixedly connected to the other end of the spring 7. A semi-circular rubber clamping plate 9 is fixedly connected to the other end of the spring rod 8. A reagent bottle 10 is movably connected to the inner wall surface of the semi-circular rubber clamping plate 9. The upper part of the inner wall surface of the reagent storage box 1 is movably connected to the handle 3. A second support 11 is connected, and a reagent dropper 12 is movably connected to the top of the second support 11. Ceramic heads 13 are movably connected to the top left and right sides of the lid 2. An ultraviolet germicidal lamp tube 14 is movably connected to the inner wall surface of the ceramic head 13. Several ventilation holes 15 are opened on the lower left and right sides of the reagent storage box 1. A condensate feeding tube 16 is fixedly connected to the lower left side of the reagent storage box 1. A circulation tube 18 is fixedly connected to the end of the condensate feeding tube 16. A circulation pump 17 is movably connected to the lower left side of the inside of the reagent storage box 1. Several cold air vents are opened on the top of the first card plate 4. 19. A condensate outlet pipe 20 is fixedly connected to the bottom right end of the circulation pipe 18. A control valve 21 is movably connected to the outer wall surface of the condensate outlet pipe 20 through the bottom right end of the reagent storage box 1. A pressure valve 22 is movably connected to the outer wall surface of the control valve 21 through a cutout. Several sealing elements 23 are provided at both ends of the reagent storage box 1. A vent sealing ring 24 is fixedly connected to the inner wall surface of the vent hole 15 on the inner side surface of the sealing element 23. A function display screen 25 is provided on the front right side of the reagent storage box 1. Several function knobs 26 are provided on the lower right side of the front of the reagent storage box 1.

[0021] The working principle of this utility model is as follows: When using this utility model, firstly, the operator holds the handle 3 and pushes upward to lift the lid 2. The operator then places the reagent bottle 10 into the reagent holder 5. Upon placement, the spring rod 8 is forced inward to compress the spring 7, allowing the semi-circular rubber clamping plate 9 to contact the reagent bottle 10. The spring 7 then rebounds, causing the spring rod 8 to push the semi-circular rubber clamping plate 9 to clamp the reagent bottle 10. Rubber pads are installed between the semi-circular rubber clamping plate 9 and the reagent bottle 10 to effectively prevent damage caused by squeezing the reagent bottle 10. The reagent dropper 12 is used to... Reagents are added or drawn into reagent bottle 10. When the rubber bulb of reagent dropper 12 is squeezed, the air inside the dropper is expelled. Then, the dropper is inserted into reagent bottle 10, and the rubber bulb is released. Because the pressure inside the dropper is lower than the pressure inside reagent bottle 10, the reagent is drawn into the dropper. When it is necessary to add reagents to other containers or perform experimental operations, the reagent can be dispensed from the dropper by squeezing the rubber bulb. The number of reagent droppers 12 is the same as that of reagent bottles 10, and they are used one-to-one to avoid confusion. The second support 11 is used to place the reagent droppers 12 corresponding to the positions of reagent bottles 10. Ultraviolet germicidal lamp. Tube 14 is installed on top of reagent storage box 1. Ceramic head 13 is used to fix the ultraviolet germicidal lamp tube 14. Turning the on / off knob of ultraviolet germicidal lamp tube 14 in function knob 26 starts ultraviolet germicidal lamp tube 14 and emits ultraviolet light. Ultraviolet light has a sterilizing and disinfecting effect, thereby killing microorganisms that may exist inside reagent storage box 1, reducing the risk of microbial contamination of NK cell amplification reagent, and ensuring the quality and safety of reagents. When it is necessary to lower the temperature inside reagent storage box 1, turn the on / off knob of circulation pump 17 in function knob 26 to circulate tube 18. The internal condensate circulation system includes ventilation holes 15 that allow adequate airflow to maintain the pressure balance within the reagent storage box 1 and help dissipate any odors or trace gases that may be generated. The ventilation sealing ring 24 of the sealing element 23 ensures that external contaminants do not enter during air exchange. The condensate feed pipe 16 is used to add new condensate. Pressing the pressure valve 22 in the control valve 21 discharges waste liquid from the condensate outlet pipe 20. The function display screen 25 displays the temperature and humidity values ​​inside the reagent storage box 1, as well as various information such as whether the circulation pump is working and its operating status.

[0022] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A NK cell expansion kit comprising a reagent storage box (1), a box cover (2), an ultraviolet sterilization lamp tube (14), a ventilation hole (15), a condensate charging pipe (16) and a control valve (21), characterized in that, The top of the reagent storage box (1) is connected with the box cover (2), the front end of the box cover (2) is transversely fixedly connected with the handle (3), the inside of the reagent storage box (1) is movably connected with the first clamping plate (4) at the lower side, the top of the first clamping plate (4) is fixedly connected with a plurality of reagent clamping seats (5), the inside of each reagent clamping seat (5) is fixedly connected with the clamping piece (6) on the left and right sides, the inner wall surface of the clamping piece (6) is fixedly connected with the spring (7), the other end of the spring (7) is fixedly connected with the spring rod (8), the other end of the spring rod (8) is fixedly connected with the semicircular rubber clamping plate (9), the inner wall surface of the semicircular rubber clamping plate (9) is movably connected with the reagent bottle (10), the inner wall surface of the reagent storage box (1) is movably connected with the second support (11) at the upper side, and the top end of the second support (11) is movably connected with the reagent dropper (12).

2. The NK cell expansion kit of claim 1, wherein, The top of the box cover (2) is movably connected with the ceramic head (13) on the left and right sides, and the inner wall surface of the ceramic head (13) is movably connected with the ultraviolet sterilization lamp tube (14).

3. The NK cell expansion kit of claim 1, wherein, A plurality of ventilation holes (15) are formed in the lower side of the left and right ends of the reagent storage box (1), and the lower side of the left end of the reagent storage box (1) is fixedly connected with the condensate feeding pipe (16).

4. The NK cell expansion kit of claim 3, wherein, The bottom end of the circulating pipe (18) is fixedly connected with the condensate outlet pipe (20).

5. The NK cell expansion kit of claim 1, wherein, The top of the first clamping plate (4) is provided with a plurality of cold air openings (19), and the bottom end of the circulating pipe (18) is fixedly connected with the condensate outlet pipe (20).

6. The NK cell expansion kit of claim 5, wherein, The outer wall surface of the condensate outlet pipe (20) is movably connected with the control valve (21) at the bottom end of the right side of the reagent storage box (1), and the outer wall surface of the control valve (21) is movably connected with the downward valve (22).

7. The NK cell expansion kit of claim 1, wherein, The left and right ends of the reagent storage box (1) are provided with a plurality of sealing elements (23), and the inner side surface of the sealing element (23) is fixedly connected with the ventilation opening sealing ring (24) on the inner wall surface of the ventilation hole (15).

8. The NK cell expansion kit of claim 1, wherein, The front end of the reagent storage box (1) is provided with a function display screen (25), and the front end of the reagent storage box (1) is provided with a plurality of function knobs (26) at the right lower side.

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