Closed sampling device

Abstract

The utility model relates to the field of biological culture, and provides a closed sampling device, which comprises a sampling pipeline provided with a first welding point, a second welding point and a third welding point, and the first welding point is used for being detachably communicated with a bioreactor; one end of the emptying mechanism is communicated with the sampling pipeline, and the other end is communicated with the outside; the waste discharge mechanism is detachably communicated with the second welding point; and the sampling mechanism is detachably communicated with the third welding point. According to the closed sampling device provided by the utility model, the sampling pipeline is provided with the first welding point which is detachably communicated with the bioreactor, the second welding point is detachably communicated with the waste discharge mechanism, the third welding point is detachably communicated with the sampling mechanism, and the emptying mechanism is communicated with the sampling pipeline; the sealing performance of the whole closed sampling device is ensured, and external pollutants are effectively prevented from entering, so that the purity and the accuracy of a sample are ensured.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of biological culture, especially a closed sampling device. BACKGROUND

[0002] In the field of biological culture and reaction, the culture process is often accompanied by a long time period, which itself makes the culture system vulnerable to external environmental pollution. More complicatedly, in order to monitor the progress and effect of the culture process, it is usually necessary to frequently sample from the culture system for detection. This sampling requirement undoubtedly further aggravates the risk of pollution, because each sampling may become a potential way for pollutants to enter the culture system.

[0003] To solve this problem, two main sampling methods have been traditionally adopted to minimize the possibility of pollution. The first method is to strictly clean and sterilize the valves and pipelines directly related to the sampling operation before and after sampling. The advantage of this method is that it can reduce the risk of introducing pollutants during sampling to a certain extent, but its disadvantage is also obvious: frequent cleaning and sterilization not only consumes time and effort, but also may cause unnecessary disturbance and damage to the culture system. The second traditional sampling method is to perform open sampling in a sterile environment, such as using a laminar flow hood or a biological safety cabinet to provide a relatively sterile operating environment. Although this method can improve the safety of sampling to a certain extent, the open operation itself is still a potential source of pollution. In addition, the maintenance of a sterile environment also requires a large amount of resources and energy input, increasing the operating cost. In addition to the above pollution risks, for some toxic, harmful or highly allergenic samples, the traditional open sampling method also poses a serious threat to the safety of the sampling personnel and the workshop environment. In the sampling process, if the sample is accidentally leaked or splashed out, not only the culture system may be contaminated, but also the health of the sampling personnel may be directly harmed. SUMMARY

[0004] The utility model provides a kind of closed sampling device, to solve the problem that sampling needs open operation in the present biological culture process, not only possibly pollutes culture system, also possibly directly harms the health of sampling personnel.

[0005] The utility model provides a kind of closed sampling device, comprising:

[0006] Sampling pipeline is equipped with first welding point, second welding point and third welding point, and the first welding point is used for being detachably communicated with biological reactor;

[0007] Evacuation mechanism, one end is communicated with the sampling pipeline, the other end is communicated with outside, and it is configured to control whether the sampling pipeline is conducted with outside;

[0008] The waste discharge mechanism is detachably communicated with the second welding point, and is configured to discharge the sample flowing out of the bioreactor through the sampling pipeline before sampling, and discharge the sample in the sampling pipeline when the sampling pipeline is communicated with the outside through the emptying mechanism.

[0009] The sampling mechanism is detachably communicated with the third welding point, and is configured to collect the sample flowing out of the bioreactor through the sampling pipeline when sampling.

[0010] According to the closed sampling device, the sampling pipeline is a four-way pipe fitting, the four-way pipe fitting is provided with a first connecting pipe section, a second connecting pipe section, a third connecting pipe section and a fourth connecting pipe section, the first welding point is arranged on the first connecting pipe section, the second welding point is arranged on the second connecting pipe section, the third welding point is arranged on the third connecting pipe section, and the fourth connecting pipe section is connected with the emptying mechanism.

[0011] According to the closed sampling device, the first connecting pipe section is provided with a liquid outlet valve, the second connecting pipe section is provided with a liquid discharge valve, and the third connecting pipe section is provided with a sampling valve.

[0012] According to the closed sampling device, the emptying mechanism comprises an emptying pipe, an emptying valve and a first air filter.

[0013] One end of the emptying pipe is communicated with the third connecting pipe section, the other end of the emptying pipe is communicated with the outside through the first air filter, and the emptying valve is arranged on the emptying pipe.

[0014] According to the closed sampling device, the waste discharge mechanism comprises a waste discharge bottle and a waste discharge pipe, and the waste discharge bottle is detachably communicated with the second welding point through the waste discharge pipe.

[0015] According to the closed sampling device, the waste discharge mechanism comprises a waste discharge bottle and a waste discharge pipe, and the waste discharge bottle is detachably communicated with the second welding point through the waste discharge pipe.

[0016] According to the closed sampling device, the waste discharge mechanism further comprises a second air filter, and the waste discharge bottle is communicated with the outside through the second air filter.

[0017] According to the closed sampling device, the sampling mechanism comprises a sampling bottle and a sampling pipe, and the sampling bottle is detachably communicated with the third welding point through the sampling pipe.

[0018] According to the closed sampling device, the sampling mechanism is provided with a plurality of sampling bottles, and at least one of the sampling bottles is detachably communicated with the third welding point through the corresponding sampling pipe.

[0019] According to the closed sampling device, the sampling mechanism further comprises a third air filter, and the sampling bottle is communicated with the outside through the third air filter.

[0020] The closed sampling device provided by the utility model realizes detachable communication of the first welding point with the bioreactor, detachable communication of the second welding point with the waste discharge mechanism, and detachable communication of the third welding point with the sampling mechanism, realizes communication of the emptying mechanism with the sampling pipeline, guarantees the sealing performance of the whole closed sampling device, effectively prevents external pollutants from entering, and thus guarantees the purity and accuracy of the sample. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical scheme in the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description.

[0022] Figure 1 It is the structural schematic diagram of the closed sampling device provided by the utility model.

[0023] Reference signs:

[0024] 10, sampling pipeline; 101, first welding point; 102, second welding point; 103, third welding point; 104, liquid outlet valve; 105, liquid discharge valve; 106, sampling valve; 20, emptying mechanism; 201, emptying valve; 202, first air filter; 30, waste discharge mechanism; 301, waste discharge bottle; 302, second air filter; 40, sampling mechanism; 401, sampling bottle; 402, third air filter; 50, bioreactor. DETAILED DESCRIPTION

[0025] To make the objectives, technical solutions, and advantages of this utility model clearer, the technical solutions of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this utility model, not all embodiments. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0026] The following is combined Figure 1 This invention describes a closed sampling device that operates in a completely sealed state, eliminating the possibility of contamination during the sampling process and maximizing the safety of sampling personnel and the workshop environment. Furthermore, it is applicable to situations where contact with air would lead to oxidation, moisture absorption, reduced activity, or other adverse effects on sample properties.

[0027] In one embodiment provided in this application, such as Figure 1 As shown, the closed sampling device includes: a sampling pipe 10, an emptying mechanism 20, a waste discharge mechanism 30, and a sampling mechanism 40. The sampling pipe 10 is provided with a first welding point 101, a second welding point 102, and a third welding point 103. The first welding point 101 is used to detachably connect to the bioreactor 50. One end of the emptying mechanism 20 is connected to the sampling pipe 10, and the other end is connected to the outside. It is configured to control whether the sampling pipe 10 is connected to the outside. The waste discharge mechanism 30 is detachably connected to the second welding point 102. It is configured to discharge the sample flowing out of the bioreactor 50 through the sampling pipe 10 before sampling, and to discharge the sample in the sampling pipe 10 when the sampling pipe 10 is connected to the outside through the emptying mechanism 20. The sampling mechanism 40 is detachably connected to the third welding point 103. It is configured to collect the sample flowing out of the bioreactor 50 through the sampling pipe 10 during sampling.

[0028] Specifically, the sampling pipe 10 has three welding points to accommodate areas that frequently require open-ended operations. These three welding points are designated as the first welding point 101, the second welding point 102, and the third welding point 103. The first welding point 101 is used to establish a detachable connection with the bioreactor 50, facilitating connection and disconnection while ensuring a tight seal at the connection. The second welding point 102 is detachably connected to the waste discharge mechanism 30. The third welding point 103 is detachably connected to the sampling mechanism 40.

[0029] Before sampling, the venting mechanism 20 is closed to cut off the airflow between the sampling pipe 10 and the external environment, ensuring the airtightness and purity of the sample during sampling. The waste discharge mechanism 30 discharges excess sample flowing out of the bioreactor 50 through the sampling pipe 10, ensuring the representativeness of the samples taken subsequently.

[0030] During sampling, the emptying mechanism 20 remains closed, the waste discharge mechanism 30 is closed, the sampling mechanism 40 collects the sample flowing out of the bioreactor 50 through the sampling pipeline 10, and the sampling mechanism 40 can be started at a predetermined sampling time point to collect a pure and accurate sample, thereby avoiding waste and pollution of the sample.

[0031] When the sampling amount is sufficient, the sampling mechanism 40 is closed, the emptying mechanism 20 is opened, the sampling pipeline 10 is connected to the outside through the emptying mechanism 20, and the waste discharge mechanism 30 can discharge the residual sample in the sampling pipeline 10 by relying on the self-gravity and atmospheric pressure of the residual sample.

[0032] The closed sampling device provided in the embodiment of the utility model, through setting first welding point 101 and bioreactor 50 can be detachably communicated on sampling pipeline 10, second welding point 102 and waste discharge mechanism 30 are detachably communicated, and third welding point 103 and sampling mechanism 40 are detachably communicated, emptying mechanism 20 and sampling pipeline 10 are communicated, the sealing property of the whole closed sampling device is guaranteed, external pollutants are effectively prevented from entering, thereby guaranteeing the purity and accuracy of the sample. The waste discharge mechanism 30 can effectively discharge the sample flowing out of the bioreactor 50 before sampling, and guarantees that the sample taken is representative. Meanwhile, during sampling, the emptying mechanism 20 can control the communication of the sampling pipeline 10 with the outside, discharge the residual sample in the pipeline, and eliminate the influence of the residual sample on the next sampling. The sampling mechanism 40 is responsible for collecting the sample at an appropriate time, thereby avoiding waste and pollution of the sample.

[0033] In some embodiments, as shown in Figure 1 The sampling pipeline 10 is a four-way pipe (cross-shaped pipe), and the four-way pipe is provided with a first connecting pipe section, a second connecting pipe section, a third connecting pipe section and a fourth connecting pipe section. The first welding point 101 is arranged on the first connecting pipe section, the second welding point 102 is arranged on the second connecting pipe section, the third welding point 103 is arranged on the third connecting pipe section, and the fourth connecting pipe section is connected with the emptying mechanism 20.

[0034] In the embodiment, the sampling pipeline 10 is cross-shaped and has a first connecting pipe section, a second connecting pipe section, a third connecting pipe section and a fourth connecting pipe section. The design of the pipe makes it possible to connect four different connecting pipe sections at a center point.

[0035] The first connecting pipe segment is provided with a first welding point 101 for detachable connection with the bioreactor 50. The first connecting point ensures that the sample in the bioreactor 50 can flow smoothly into the sampling pipe 10. The second connecting pipe segment is provided with a second welding point 102 for detachable connection with the waste discharge mechanism 30. The waste discharge mechanism 30 can discharge the residual or unqualified sample in the pipe through the second connecting point. The third connecting pipe segment is provided with a third welding point 103 for detachable connection with the sampling mechanism 40. The sampling mechanism 40 can collect the pure sample from the bioreactor 50 through the third connecting point. The fourth connecting pipe segment is connected with the emptying mechanism 20. The fourth connecting point allows the emptying mechanism 20 to control the opening and closing of the sampling pipe 10 to the outside world during sampling, so that the emptying mechanism 20 remains closed before and during sampling to avoid contamination of the sampling pipe 10 by the outside world. When the sampling amount is sufficient, the emptying mechanism 20 is opened, and the sampling pipe 10 is connected to the outside world through the emptying mechanism 20, and the residual sample is discharged from the sampling pipe 10 by the waste discharge mechanism 30 relying on its own gravity and atmospheric pressure.

[0036] In some embodiments, as shown in FIG. 1, a liquid outlet valve 104 is arranged on the first connecting pipe segment, a liquid discharge valve 105 is arranged on the second connecting pipe segment, and a sampling valve 106 is arranged on the third connecting pipe segment. Figure 1

[0037] Specifically, the liquid outlet valve 104 is arranged on the first connecting pipe segment and is used to control the flow of the sample in the bioreactor 50 into the sampling pipe 10. When sampling is desired, the liquid outlet valve 104 should be in an open state so that the sample can flow smoothly into the pipe. The liquid discharge valve 105 is arranged on the second connecting pipe segment and is connected with the waste discharge mechanism 30. Before and after sampling, the liquid discharge valve 105 can be used to control the discharge of the excess or residual sample in the pipe. By opening the liquid discharge valve 105, the waste discharge mechanism 30 ensures that the sample taken is representative and that the residual sample in the pipe is discharged, ensuring the purity and representativeness of subsequent sampling. The sampling valve 106 is arranged on the third connecting pipe segment and is connected with the sampling mechanism 40. During sampling, the sampling valve 106 should be in an open state so that the sampling mechanism 40 can collect the sample from the bioreactor 50. After sampling is completed, the sampling valve 106 is closed to prevent leakage of the sample.

[0038] In actual use, the liquid outlet valve 104 and the liquid discharge valve 105 are first opened, and the sample in the bioreactor 50 flows out under tank pressure or relying on its own gravity and enters the waste discharge mechanism 30. After discharging a part, the liquid discharge valve 105 is closed, the sampling valve 106 is opened, the sample enters the sampling mechanism 40, and when the sampling amount is sufficient, the tank sampling valve 106 and the liquid outlet valve 104 are closed. The emptying valve 201 and the liquid discharge valve 105 are opened, and the residual sample in the sampling pipe 10 is discharged to the waste discharge mechanism 30 relying on its own gravity. Finally, the emptying valve 201 and the liquid discharge valve 105 are closed, and the sampling is completed.​

[0039] In some embodiments, such as Figure 1 As shown, the venting mechanism 20 includes: a venting pipe, a venting valve 201, and a first air filter 202; one end of the venting pipe is connected to the third connecting pipe section, and the other end of the venting pipe is connected to the outside through the first air filter 202; the venting valve 201 is installed on the venting pipe.

[0040] Specifically, the vent pipe serves as a channel connecting the sampling pipeline 10 to the outside world. One end of the vent pipe is connected to the third connecting pipe section, while the other end is connected to the external environment through the first air filter 202. The vent valve 201 controls the opening and closing of the vent pipe, thereby controlling the connection status between the sampling pipeline 10 and the outside world. The first air filter 202 prevents pollutants (such as dust, microorganisms, etc.) from the external environment from entering the sampling pipeline 10 or the bioreactor 50 through the vent pipe.

[0041] In some embodiments, such as Figure 1 As shown, the waste discharge mechanism 30 includes a waste discharge bottle 301 and a waste discharge pipe; the waste discharge bottle 301 is detachably connected to the second welding point 102 via the waste discharge pipe. The waste discharge bottle 301 serves as a container for collecting samples discharged from the bioreactor 50, and its material can be glass, PP, PC, stainless steel, ceramic, etc. It typically has sufficient volume to hold the discharged samples and is designed to be sealable to prevent sample leakage or evaporation. The waste discharge pipe serves as a channel connecting the sampling pipe 10 and the waste discharge bottle 301, used to discharge samples from the sampling pipe 10 and introduce them into the waste discharge bottle 301. The connection between the waste discharge pipe and the second welding point 102 is designed to be detachable, which facilitates the replacement of the waste discharge bottle 301. If a single waste discharge mechanism 30 is insufficient for continuous operation, multiple waste discharge mechanisms 30 may be provided.

[0042] Each waste discharge mechanism 30 includes a waste discharge bottle 301 and a waste discharge pipe. The waste discharge bottle 301 is detachably connected to the second welding point 102 via a corresponding waste discharge pipe. This detachable connection design facilitates the replacement of the waste discharge bottle 301.

[0043] When the waste discharge mechanism 30 needs to be replaced, the waste discharge pipe on the new waste discharge mechanism 30 is welded to the second welding point 102, and the old waste discharge mechanism 30 is replaced. In this way, the sample in the bioreactor 50 can flow out through the new waste discharge pipe and be collected in the new waste discharge bottle 301.

[0044] Optionally, the waste discharge mechanism 30 further includes a second air filter 302. The waste discharge bottle 301 communicates with the outside environment through the second air filter 302. The second air filter 302 is installed at the exhaust port of the waste discharge bottle 301. The second air filter 302 is used to balance the air pressure inside and outside the waste discharge mechanism 30. In some embodiments, such as Figure 1As shown, the sampling mechanism 40 includes a sampling bottle 401 and a sampling tube; the sampling bottle 401 is detachably connected to the third welding point 103 via the sampling tube. The sampling bottle 401 is a container for collecting samples taken from the bioreactor 50. It is typically designed to be well-sealed to prevent leakage or contamination of the sample during storage or transportation. The material of the sampling bottle 401 should be able to withstand the chemical properties that the sample may possess and meet relevant safety and hygiene standards; the material can be glass, PP, PC, stainless steel, ceramic, etc. The sampling tube is a channel connecting the third welding point 103 and the sampling bottle 401. The sampling tube allows operators to extract samples from the bioreactor 50 and transfer them to the sampling bottle 401. The design of the sampling tube should take into account the sample's flowability, viscosity, and potential corrosivity to ensure smooth and undamaged sample transfer.

[0045] Since multiple samplings are required during the production process, there are generally multiple sampling mechanisms 40, and at least one of the sampling mechanisms 40 has a sampling bottle 401 that is detachably connected to the third welding point 103 through a corresponding sampling tube.

[0046] Each sampling device 40 includes a sampling bottle 401 and a sampling tube. The sampling bottle 401 is detachably connected to the third welding point 103 via a corresponding sampling tube. This detachable connection design facilitates the replacement of the sampling bottle 401.

[0047] When it is necessary to switch sampling mechanisms 40, the sampling tube of the sampling mechanism 40 to be switched is welded to the third welding point 103, and the old sampling mechanism 40 is replaced. In this way, the sample in the bioreactor 50 can flow out through the new sampling tube and be collected in the new sampling bottle 401.

[0048] Optionally, the sampling mechanism 40 further includes a third air filter 402; the sampling bottle 401 is connected to the outside through the third air filter 402. The third air filter 402 is installed at the exhaust port of the sampling bottle 401. The third air filter 402 is used to balance the air pressure inside and outside the sampling mechanism 40.

[0049] When the sampling mechanism 40 is not connected to the third welding point 103, a third air filter 402 can be installed on the sampling tube to facilitate sterilization.

[0050] In one specific embodiment, such as Figure 1 As shown, the container preparation is performed first: the required sampling pipeline 10 (including the emptying mechanism 20), waste discharge mechanism 30, and sampling mechanism 40 are cleaned and sterilized by moist heat, dry heat, or irradiation before use. The waste discharge mechanism 30 is welded to the second welding point 102, and the sampling mechanism 40 is welded to the third welding point 103. The bioreactor 50 is welded to the first welding point 101, and the liquid outlet valve 104, liquid drain valve 105, sampling valve 106, and emptying valve 201 are closed.

[0051] In actual use, first open the outlet valve 104 and the drain valve 105, the sample in the bioreactor 50 flows out under the condition of tank pressure or self gravity, and enters the waste disposal mechanism 30. After discharging a part, close the drain valve 105, open the sampling valve 106, and the sample enters the sampling mechanism 40. When the sampling amount is sufficient, close the tank sampling valve 106 and the outlet valve 104. Open the emptying valve 201 and the drain valve 105, and the residual sample in the sampling pipeline 10 is emptied to the waste disposal mechanism 30 under the condition of self gravity. Finally, close the emptying valve 201 and the drain valve 105, and the sampling is completed.

[0052] When the sampling mechanism 40 needs to be replaced, the sampling tube of the sampling mechanism 40 to be used and the sampling tube of the sampling mechanism 40 that has finished sampling are clamped by the pipe connecting machine, the heating blade is cut by the pipe connecting machine, the connection is converted, the new connection pipeline is welded, and cooling is performed, so that the sampling mechanism 40 that has finished sampling is taken off from the sampling pipeline 10, and the sampling tube of the sampling mechanism 40 to be used is connected to the sampling pipeline 10.

[0053] The replacement of the waste disposal mechanism 30 is the same as the operation of the sampling mechanism 40, and the waste disposal mechanism 30 filled with waste liquid can be taken off, and the new waste disposal mechanism 30 is connected to the sampling pipeline 10. If the sampling mechanism 40 and the waste disposal mechanism 30 need to be taken off, and the new sampling mechanism 40 and the waste disposal mechanism 30 do not need to be connected, the pipe connecting machine can be connected to the closed pipeline to block the sampling pipeline 10.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that the technical solutions recorded in the foregoing embodiments can still be modified, or some technical features can be replaced by equivalents; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A closed sampling device, characterized in that The application relates to a sampling device for a bioreactor, comprising: a sampling pipeline (10) provided with a first welding point (101), a second welding point (102) and a third welding point (103), wherein the first welding point (101) is used for detachably communicating with a bioreactor (50); an emptying mechanism (20) communicating with the sampling pipeline (10) at one end and communicating with the outside at the other end, and configured to control whether the sampling pipeline (10) is in communication with the outside; a waste discharge mechanism (30) detachably communicating with the second welding point (102), and configured to discharge the sample flowing out of the bioreactor (50) through the sampling pipeline (10) before sampling, and discharge the sample in the sampling pipeline (10) when the sampling pipeline (10) is in communication with the outside through the emptying mechanism (20); a sampling mechanism (40) detachably communicating with the third welding point (103), and configured to collect the sample flowing out of the bioreactor (50) through the sampling pipeline (10) during sampling.

2. The closed sampling device of claim 1, wherein The sampling pipeline (10) is a four-way pipe, which is provided with a first connecting pipe section, a second connecting pipe section, a third connecting pipe section and a fourth connecting pipe section; the first welding point (101) is arranged on the first connecting pipe section, the second welding point (102) is arranged on the second connecting pipe section, the third welding point (103) is arranged on the third connecting pipe section, and the fourth connecting pipe section is connected with the emptying mechanism (20).

3. The closed sampling device of claim 2, wherein, A liquid outlet valve (104) is arranged on the first connecting pipe section, a liquid discharge valve (105) is arranged on the second connecting pipe section, and a sampling valve (106) is arranged on the third connecting pipe section.

4. The closed sampling device of claim 3, wherein The emptying mechanism (20) comprises an emptying pipeline, an emptying valve (201) and a first air filter (202); one end of the emptying pipeline communicates with the third connecting pipe section, the other end of the emptying pipeline is in communication with the outside through the first air filter (202), and the emptying valve (201) is arranged on the emptying pipeline.

5. The closed sampling device of claim 1, wherein The waste discharge mechanism (30) comprises a waste discharge bottle (301) and a waste discharge pipeline; the waste discharge bottle (301) is detachably communicated with the second welding point (102) through the waste discharge pipeline.

6. The closed sampling device of claim 5, wherein The waste discharge mechanism (30) is provided with a plurality of waste discharge bottles (301), and at least one waste discharge bottle (301) in the waste discharge mechanism (30) is detachably communicated with the second welding point (102) through a corresponding waste discharge pipeline.

7. The closed sampling device of claim 5, wherein The waste discharge mechanism (30) further comprises a second air filter (302); the waste discharge bottle (301) is in communication with the outside through the second air filter (302).

8. The closed sampling device of claim 1, wherein The sampling mechanism (40) comprises a sampling bottle (401) and a sampling pipeline; the sampling bottle (401) is detachably communicated with the third welding point (103) through the sampling pipeline.

9. The closed sampling device of claim 8, wherein, The sampling mechanism (40) is provided with a plurality of sampling bottles (401), and at least one sampling bottle (401) is detachably communicated with the third welding point (103) through a corresponding sampling pipeline.

10. The closed sampling device of claim 8, wherein The sampling mechanism (40) further comprises a third air filter (402); the sampling bottle (401) communicates with the outside through the third air filter (402).

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