Magnetic separation module and high-speed full-automatic chemiluminescence immunoassay analyzer

Abstract

The utility model belongs to the technical field of immunochemical analysis and detection, and particularly relates to a magnetic separation module and a high-speed full-automatic chemiluminescence immunoassay instrument. The magnetic separation module comprises a magnetic separation bottom plate assembly, a magnetic separation pot assembly and a magnetic separation liquid suction needle assembly are arranged on the upper top face of the magnetic separation bottom plate assembly, and a magnetic separation uniform mixing assembly is arranged below the magnetic separation bottom plate assembly. The utility model relates to a high-speed full-automatic chemiluminescence immunoassay analyzer, which comprises a magnetic separation module.

Description

Technical Field

[0001] This utility model belongs to the technical field of immunochemical analysis and detection technology, specifically relating to a magnetic separation module and a high-speed fully automated chemiluminescence immunoassay analyzer. Background Technology

[0002] A chemiluminescence immunoassay analyzer is an analytical instrument that combines chemiluminescence analysis and immunoassay. It utilizes the specific binding reaction between antigens and antibodies, combined with chemiluminescence technology, to detect the concentration of specific substances in a sample. For example, when detecting thyroid hormones in the blood, the thyroid hormone (antigen) specifically binds to the corresponding thyroid hormone antibody in the instrument. Its immunological principle is based on the specific binding property of antigens and antibodies. The sample to be tested reacts with an antibody (or antigen) labeled with a chemiluminescent substance to form an antigen-antibody complex.

[0003] The analytical steps using a chemiluminescence immunoassay analyzer are as follows: First, the sample to be tested is pretreated; for example, biological samples such as blood and urine may require centrifugation and dilution. Then, the pretreated sample undergoes an immunoassay with antibodies labeled with chemiluminescent substances, forming antigen-antibody complexes. Next, unbound substances are removed through washing to reduce non-specific background signals. Then, the substrate that generates the chemiluminescence reaction is added to the reaction system; the substrate reacts with the labeled enzyme to produce a chemiluminescent signal. Finally, an optical detector such as a photomultiplier tube or photodiode converts the chemiluminescent signal into an electrical signal for detection and analysis, yielding information such as the concentration of the target substance in the sample. Due to its significant advantages of high sensitivity, high specificity, wide linear range, and rapid detection, chemiluminescence immunoassay analyzers are widely used in clinical diagnostics and scientific research.

[0004] Based on its structure, a chemiluminescence immunoassay analyzer includes a sample processing system, a reagent storage and dispensing system, a reaction system, a detection system, and a computer control system. The reaction system includes the reagent tray module, sample needle module, and magnetic separation module mentioned in this solution. Currently, most commercially available magnetic separation modules employ a structure that simultaneously mixes the washing solution and substrate, which struggles to simultaneously meet the required mixing strength for both. The drawbacks of simultaneous mixing of the washing solution and substrate are significant, primarily including: 1. Risk of cross-contamination; 2. Reduced reaction stability; 3. Unsatisfactory mixing effect; 4. Increased instrument maintenance difficulty.

[0005] In order to enable the cleaning solution and substrate to be isolated and mixed with different strengths, this invention aims to provide a magnetic separation module and a high-speed fully automated chemiluminescence immunoassay analyzer. Utility Model Content

[0006] The purpose of this invention is to provide a magnetic separation module and a high-speed fully automated chemiluminescence immunoassay analyzer to solve the problems mentioned in the background art.

[0007] To achieve the above objectives, this utility model provides the following technical solution:

[0008] A magnetic separation module includes a magnetic separation base plate assembly, wherein a magnetic separation pot assembly and a magnetic separation liquid suction needle assembly are disposed on the upper top surface of the magnetic separation base plate assembly, and a magnetic separation mixing assembly is disposed on the lower part of the magnetic separation base plate assembly.

[0009] The magnetic separation pot assembly includes a magnetic separation base, a magnetic separation disc cover, a rotating disc, a rotating disc bearing, a main drive pulley, and a main drive motor. The magnetic separation base is fixed to the upper surface of the magnetic separation base plate assembly. A rotating disc bearing is located at the magnetic separation base; the upper end of the rotating disc bearing is fixedly connected to the rotating disc, and the lower end of the rotating disc bearing is fixedly connected to the main drive pulley. The main drive motor is fixed to the lower surface of the magnetic separation base plate assembly, and the main drive motor and the main drive pulley are connected in a driving connection. The rotating disc has several reaction cup placement slots evenly distributed in a circular array. The tank contains a reaction cup; the magnetic separation disk cover has a cup changing port, a cleaning fluid injection needle, several cleaning fluid mixing capping assemblies and a substrate mixing capping assembly arranged in a circular array; the cleaning fluid mixing capping assembly includes a cleaning fluid suction needle assembly and a cleaning fluid injection needle assembly, and the substrate mixing capping assembly includes a substrate suction needle assembly and a substrate mixing capping assembly; as the rotating disk rotates through several stations, the cup changing port, the cleaning fluid injection needle, the cleaning fluid suction needle assembly, the cleaning fluid injection needle assembly, the substrate suction needle assembly and the substrate mixing capping assembly are always located directly above one of the reaction cup placement tanks;

[0010] The magnetic separation liquid suction needle assembly includes a lifting mechanism and a liquid suction needle fixing plate. The lifting mechanism is provided between the magnetic separation base plate assembly and the liquid suction needle fixing plate. The liquid suction needle fixing plate is located directly above the magnetic separation pot assembly. The cleaning fluid liquid suction needle assembly includes a cleaning fluid liquid suction needle, and the substrate liquid suction needle assembly includes a substrate liquid suction needle. The upper half of the cleaning fluid liquid suction needle and the upper half of the substrate liquid suction needle are both fixed to the liquid suction needle fixing plate.

[0011] The magnetic separation and mixing assembly includes a C-shaped fixing plate, several cleaning fluid mixing columns, a substrate mixing column, a second elevator, a cleaning fluid mixing motor, and a substrate mixing motor. The several cleaning fluid mixing columns and substrate mixing columns are arranged sequentially at the C-shaped fixing plate. The several cleaning fluid mixing columns and several cleaning fluid injection needle assemblies correspond one-to-one and are located on the same vertical axis. The substrate mixing columns and the substrate mixing cap are located on the same vertical axis. A cleaning fluid mixing synchronization belt is provided between the cleaning fluid mixing motor and the several cleaning fluid mixing columns, and a substrate mixing synchronization belt is provided between the substrate mixing motor and the substrate mixing columns. A second elevator is provided between the C-shaped fixing plate and the magnetic separation base plate assembly.

[0012] Preferably, the cleaning fluid suction needle assembly includes a cleaning fluid suction needle, the upper half of which is fixed to a suction needle fixing plate, and the lower half of which can penetrate into the magnetic separation pot assembly; as the rotating disk rotates through several stations, the lower end of the cleaning fluid suction needle is always aligned with one of the reaction cup placement slots.

[0013] Preferably, the cleaning fluid injection needle assembly includes a cleaning fluid injection needle, a mounting base, a bearing, a rotating hollow shaft, a return spring, and a capping head. The mounting base has a bearing, the lower end of which is connected to the rotating hollow shaft. The lower end of the rotating hollow shaft is nested with the capping head. A return spring is located inside the rotating hollow shaft, with its two ends abutting against the inner wall of the rotating hollow shaft and the top of the capping head, respectively. The cleaning fluid injection needle passes sequentially through the bearing and the rotating hollow shaft, and its end is connected to the capping head. A guide pin is provided on the outer wall of the capping head, and correspondingly, a guide groove is provided on the side wall of the rotating hollow shaft. After the rotating disk rotates through several stations, the lower end of the capping head always aligns with one of the reaction cup placement slots.

[0014] Preferably, the substrate aspiration needle assembly includes a substrate aspiration needle and a stabilizing seat. The upper half of the substrate aspiration needle is fixed to the aspiration needle fixing plate, and the lower half of the substrate aspiration needle passes through the stabilizing seat. After the rotating disk rotates for several positions, the lower end of the substrate aspiration needle is always aligned with one of the reaction cup placement slots.

[0015] Preferably, the substrate mixing cap includes a bearing, a capping head, and a return spring. The bearing is mounted on the magnetic separation disc cap, the capping head passes through the bearing, and a return spring is provided between the bearing and the capping head. A guide pin is provided at the bearing, and a guide groove is provided at the capping head.

[0016] Preferably, the lifting platform includes a fixed rail, a sliding rail, and a lifting motor. The fixed rail is fixedly mounted on the magnetic separation base plate assembly, the lifting motor is fixedly mounted below the fixed rail, and the sliding rail is in a limiting fit with the fixed rail, allowing the sliding rail to slide up and down along the fixed rail. A suction needle fixing plate is fixedly connected to the upper end of the sliding rail, and a lifting worm gear is provided at the lower end of the sliding rail, which is connected to the lifting motor.

[0017] Preferably, the second lifting platform includes a second fixed track, a second sliding track, and a second lifting motor; the second fixed track is fixedly mounted on the magnetic separation base plate assembly, the second sliding track is fixedly mounted on the C-shaped fixed plate, the second sliding track and the second fixed track are in a limiting fit, and the second sliding track can slide along the second fixed track; the second lifting motor is fixedly mounted below the second fixed track, and the second sliding track is provided with a second lifting worm gear, which is connected to the second lifting motor.

[0018] Preferably, the cleaning fluid mixing column is rotatably connected to a C-shaped fixing plate, and a reaction cup fixing groove is provided at the top of the cleaning fluid mixing column; a cleaning fluid mixing driven wheel is provided at the bottom of the cleaning fluid mixing column, and a cleaning fluid mixing synchronous belt is provided between the cleaning fluid mixing motor and several cleaning fluid mixing driven wheels; several cleaning fluid mixing tensioning wheels are provided on the lower surface of the C-shaped fixing plate, and the several cleaning fluid mixing tensioning wheels are connected to the cleaning fluid mixing synchronous belt.

[0019] Preferably, the substrate mixing column is rotatably connected to the C-shaped fixing plate, and the top of the substrate mixing column is provided with a reaction cup fixing groove; the bottom of the substrate mixing column is provided with a substrate mixing driven wheel, and a substrate mixing synchronous belt is provided between the substrate mixing motor and several substrate mixing driven wheels; several substrate mixing tensioning wheels are provided on the lower surface of the C-shaped fixing plate, and the several substrate mixing tensioning wheels are connected to the substrate mixing synchronous belt.

[0020] A high-speed, fully automated chemiluminescence immunoassay analyzer includes a magnetic separation module.

[0021] Compared with the prior art, the beneficial effects of this utility model are:

[0022] (1) The present invention provides a magnetic separation module, the magnetic separation mixing component includes three cleaning liquid mixing columns and one substrate mixing column, the three cleaning liquid mixing columns are connected to the same cleaning liquid mixing motor, the substrate mixing column is connected to the substrate mixing motor, and a rotating disk structure is adopted. The reaction cup can complete the mixing operation of the cleaning liquid and the substrate in sequence during the rotation of the rotating disk. The cleaning liquid and the substrate can be mixed separately to achieve different mixing intensity requirements.

[0023] (2) The magnetic separation module provided by this utility model adopts a square reaction cup structure. When the square reaction cup rotates under the drive of the mixing drive column, the square reaction cup can achieve self-rotation mixing and the mixing consistency is good.

[0024] (3) The magnetic separation module provided by this utility model uses a magnetic separation mixing component to move upward, lift the corresponding reaction cup, and then the corresponding capping head covers the reaction cup. At this time, liquid injection and self-rotation mixing begin. After self-rotation mixing, the reaction cup is transferred to the next cleaning liquid aspiration needle component or substrate aspiration needle component for liquid aspiration. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of a high-speed, fully automated chemiluminescence immunoassay analyzer.

[0026] Figure 2 This is a disassembled structural diagram of a high-speed, fully automated chemiluminescence immunoassay analyzer.

[0027] Figure 3 This is a schematic diagram of the magnetic separation module.

[0028] Figure 4 Schematic diagram of the disassembly structure of the magnetic separation module Figure 1 ;

[0029] Figure 5 Schematic diagram of the disassembly structure of the magnetic separation module Figure 2 ;

[0030] Figure 6 This is a side-bottom view of the magnetic separation module;

[0031] Figure 7 Schematic diagram of the magnetic separation and mixing component Figure 1 ;

[0032] Figure 8 This is a schematic diagram of the magnetic separation disk cover.

[0033] Figure 9 Schematic diagram of the magnetic separation and mixing component Figure 2 ;

[0034] Figure 10 A schematic diagram of the structure of the reaction vessel, the washing solution mixing column / substrate mixing column;

[0035] Figure 11 A cross-sectional view of the cleaning fluid injection needle assembly;

[0036] Figure 12 A cross-sectional view of the substrate being mixed and capped;

[0037] In the diagram: Sample Management Module 1; Sample Transfer Module 2; Computer Module 3; Host Module 4; Reagent Tray Module 5; Sample Needle Module 6; Arm Assembly Module 7; Magnetic Separation Module 8; Photometric Module 9; Reagent Cup Module 10; Reaction Cup 11; Magnetic Separation Base Plate Assembly 12; Magnetic Separation Pot Assembly 13; Magnetic Separation Aspiration Needle Assembly 14; Magnetic Separation Mixing Assembly 15; Magnetic Separation Base 16; Magnetic Separation Plate Cover 17; Rotating Plate 18; Rotating Plate Bearing 19; Main Drive Pulley 20; Main Drive Motor 21; Lifting Mechanism 1 22; Aspiration Needle Fixing Plate 23; C-Shaped Fixing Plate 24; Cleaning Solution Mixing Column 25; Substrate Mixing Column 26; Lifting Mechanism 27; Cleaning Solution Mixing Motor 28; Substrate Mixing Motor 29; Reaction Cup Placement Slot 30; Fixed Rail 1 31; Sliding Rail 1 32; Lifting Motor 1 33; Lifting worm gear one; 34; Cup changing mouth; 35; Cleaning fluid injection needle; 36; Cleaning fluid mixing cap assembly; 37; Substrate mixing cap assembly; 38; Cleaning fluid suction needle assembly; 39; Cleaning fluid injection needle assembly; 40; Cleaning fluid suction needle; 41; Mounting base; 42; Bearing; 43; Rotating hollow shaft; 44; Return spring; 45; Capping head; 46; Guide pin; 47; Guide groove; 48; Lifting worm gear two; 49; Substrate suction needle assembly; 50; Substrate mixing cap; 51; Substrate suction needle; 52; Stabilizing seat; 53; Cleaning fluid mixing synchronous belt; 54; Substrate mixing synchronous belt; 55; Cleaning fluid mixing driven wheel; 56; Cleaning fluid mixing tension wheel; 57; Substrate mixing driven wheel; 58; Substrate mixing synchronous belt; 59; Substrate mixing tension wheel; 60; Fixed track two; 61; Sliding track two; 62; Lifting motor two; 63. Detailed Implementation

[0038] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0039] A high-speed, fully automated chemiluminescence immunoassay analyzer includes a sample management module 1, a sample transfer module 2, a computer module 3, and a host module 4. Samples are placed into the sample management module 1 and then transferred to the host module 4 by the sample transfer module 2, where they are controlled and detected by the computer module 3. The host module 4 includes a reagent tray module 5, a sample needle module 6, two arm modules 7, a magnetic separation module 8, a photometric module 9, and a reaction cup module 10. Reaction cups 11 are introduced into the sample needle module 6 via the reaction cup module 10. Reagents are then removed from the reagent tray module 5 and added to the reaction cups 11 of the sample needle module 6. Reaction incubation takes place within the reaction cups 11 of the sample needle module 6. After incubation, one of the arm modules 7 transports the reaction cups 11 of the sample needle module 6 to the magnetic separation module 8. After the magnetic separation module 8 completes its operation, the other arm module 7 transports the reaction cups 11 to the photometric module 9 for detection.

[0040] The magnetic separation module 8 includes a magnetic separation base plate assembly 12, with a magnetic separation pot assembly 13 and a magnetic separation suction needle assembly 14 located on the top surface of the magnetic separation base plate assembly 12. A magnetic separation mixing assembly 15 is located below the magnetic separation pot assembly 13. The magnetic separation pot assembly 13 includes a magnetic separation base 16, a magnetic separation disc cover 17, a rotating disc 18, a rotating disc bearing 19, a main drive pulley 20, and a main drive motor 21. The magnetic separation suction needle assembly 14 includes a lifting mechanism 22 and a suction needle fixing plate 23. The magnetic separation mixing assembly 15 includes a C-shaped fixing plate 24, three cleaning fluid mixing columns 25, a substrate mixing column 26, a second lifting mechanism 27, a cleaning fluid mixing motor 28, and a substrate mixing motor 29.

[0041] A magnetic separation base 16 is fixed to the upper surface of the magnetic separation base plate assembly 12. A rotating disk bearing 19 is provided at the magnetic separation base 16. The upper end of the rotating disk bearing 19 is fixedly connected to the rotating disk 18, and the lower end of the rotating disk bearing 19 is fixedly connected to the main drive pulley 20. A main drive motor 21 is fixed to the lower surface of the magnetic separation base plate assembly 12, and the main drive motor 21 and the main drive pulley 20 are connected in a driving manner. When the main drive motor 21 operates, it drives the rotating disk 18 to rotate around its axis. The rotating disk 18 has thirty-six reaction cup placement slots 30 evenly distributed in a circular array, and reaction cups 11 are placed in each reaction cup placement slot 30. Each reaction cup placement slot 30 is a workstation. The rotating disk 18 rotates clockwise, passing one workstation at a time.

[0042] The suction needle fixing plate 23 is located directly above the magnetic separation disc cover 17. A lifting mechanism 22 is provided between the magnetic separation base plate assembly 12 and the suction needle fixing plate 23. The lifting mechanism 22 includes a fixed rail 31, a sliding rail 32, and a lifting motor 33. The fixed rail 31 is fixedly installed on the magnetic separation base plate assembly 12, and the lifting motor 33 is fixedly installed below the fixed rail 31. The sliding rail 32 is in a limiting fit with the fixed rail 31 and can slide up and down along the fixed rail 31. The suction needle fixing plate 23 is fixedly connected to the upper end of the sliding rail 32, and a lifting worm gear 34 is provided at the lower end of the sliding rail 32. The lifting worm gear 34 is connected to the lifting motor 33. Driven by the lifting motor 33, the suction needle fixing plate 23 can move up and down.

[0043] The magnetic separation disk cover 17 is equipped with a cup changing port 35, a cleaning fluid injection needle 36, three cleaning fluid mixing capping assemblies 37, and a substrate mixing capping assembly 38 arranged in a circular array. The cup changing port 35 is used to change the reaction cups 11 on the rotating disk 18. As the rotating disk 18 rotates one station, a reaction cup placement slot 30 is always located directly below the cup changing port 35, facilitating the replacement of the corresponding reaction cups 11 by the preceding and following arm modules 7. A cleaning fluid injection needle 36 is located one station away from the cup changing port 35, injecting cleaning fluid into the newly placed reaction cup 11. The three cleaning fluid mixing capping assemblies 37 are arranged sequentially after the cleaning fluid injection needle 36.

[0044] The cleaning fluid mixing and capping assembly 37 includes a cleaning fluid suction needle assembly 39 and a cleaning fluid injection needle assembly 40. The cleaning fluid suction needle assembly 39 includes a cleaning fluid suction needle 41, the upper half of which is fixed to a suction needle fixing plate 23, and the lower half of which can penetrate into the magnetic separation pot assembly 13. The cleaning fluid suction needle 41 can move up and down with the suction needle fixing plate 23. After the rotating disk 18 rotates one position, the lower end of the cleaning fluid suction needle 41 is always aligned with the reaction cup 11 of one of the reaction cup placement slots 30, so that the cleaning fluid suction needle 41 can draw cleaning fluid from the corresponding reaction cup 11. The cleaning fluid injection needle assembly 40 includes a cleaning fluid injection needle 36, a mounting base 42, a bearing 43, a rotating hollow shaft 44, a return spring 45, and a capping head 46. The mounting base 42 houses the bearing 43, the lower end of which is connected to the rotating hollow shaft 44. The lower end of the rotating hollow shaft 44 is nested within the capping head 46. The return spring 45 is located inside the rotating hollow shaft 44, with its two ends abutting against the inner wall of the rotating hollow shaft 44 and the top of the capping head 46, respectively. The cleaning fluid injection needle 36 passes sequentially through the bearing 43 and the rotating hollow shaft 44, with its end connected to the capping head 46. As the rotating disk 18 rotates one position, the lower end of the capping head 46 remains aligned with one of the reaction cup placement slots 30. The lower end of the capping head 46 abuts against the top of the reaction cup 11. While injecting cleaning fluid into the reaction cup 11 through the capping head 46, the capping head 46 covers the reaction cup 11, enabling rotational mixing and effectively preventing the cleaning fluid from overflowing during the rotational mixing process. To stabilize the rotation of the capping head 46 during its rotation, a guide pin 47 is provided on the outer wall of the capping head 46. Correspondingly, a guide groove 48 is provided on the side wall of the rotating hollow shaft 44.

[0045] The substrate mixing capping assembly 38 includes a substrate suction needle assembly 50 and a substrate mixing cap 51, which are positioned one station apart. As the rotating disk 18 rotates one station, the lower ends of the substrate suction needle assembly 50 and the substrate mixing cap 51 are always aligned with the reaction cup 11 in one of the reaction cup placement slots 30. After the substrate suction needle assembly 50 has absorbed the cleaning solution from the corresponding reaction cup 11, the reaction cup 11 is transferred to the next station, where it is covered by the substrate mixing cap 51 for rotational mixing. The substrate suction needle assembly 50 includes a substrate suction needle 52 and a stabilizing seat 53. The upper half of the substrate suction needle 52 is fixed to the suction needle fixing plate 23, and the lower half of the substrate suction needle 52 passes through the stabilizing seat 53. The substrate mixing cap 51 includes a bearing 43, a capping head 46, and a return spring 45. The bearing 43 is installed on the magnetic separation disc cover 17. The capping head 46 passes through the bearing 43. The return spring 45 is provided between the bearing 43 and the capping head 46. A guide pin 47 is provided at the bearing 43, and a guide groove 48 is provided at the capping head 46.

[0046] The magnetic separation and mixing assembly 15 includes a C-shaped fixing plate 24, three cleaning fluid mixing columns 25, one substrate mixing column 26, a lifting mechanism 27, a cleaning fluid mixing motor 28, and a substrate mixing motor 29. The three cleaning fluid mixing columns 25 and the three cleaning fluid injection needle assemblies 40 are corresponding one-to-one and located on the same vertical axis. The substrate mixing column 26 and the substrate mixing cap 51 are located on the same vertical axis. The three cleaning fluid mixing columns 25 and the substrate mixing column 25 are arranged sequentially at the C-shaped fixing plate 24. The cleaning fluid mixing columns 25 are rotatably connected to the C-shaped fixing plate 24, and the substrate mixing column 26 is rotatably connected to the C-shaped fixing plate 24. To drive the cleaning fluid mixing columns 25 and the substrate mixing column 26 to rotate, a cleaning fluid mixing synchronous belt 54 is provided between the cleaning fluid mixing motor 28 and the three cleaning fluid mixing columns 25, and a substrate mixing synchronous belt 55 is provided between the substrate mixing motor 29 and the substrate mixing column 26. The bottom of the cleaning fluid mixing column 25 is equipped with a cleaning fluid mixing driven wheel 56. A cleaning fluid mixing motor 28 is connected to the three cleaning fluid mixing driven wheels 56 via a cleaning fluid mixing synchronous belt 54. Simultaneously, two cleaning fluid mixing tension wheels 57 are located on the lower surface of the C-shaped fixing plate 24, and these tension wheels 57 are connected to the cleaning fluid mixing synchronous belt 54. The bottom of the substrate mixing column 26 is equipped with a substrate mixing driven wheel 58. A substrate mixing synchronous belt 59 is connected to the substrate mixing driven wheel 28 via a substrate mixing motor 29. Two substrate mixing tension wheels 60 are located on the lower surface of the C-shaped fixing plate 24, and these tension wheels 60 are connected to the substrate mixing synchronous belt 59. The cleaning fluid mixing motor 28 drives the three cleaning fluid mixing columns 25 to rotate synchronously, and the substrate mixing motor 29 drives the substrate mixing column 26 to rotate. A reaction cup fixing groove 30 is provided at the top of the cleaning fluid mixing column 25 and the top of the substrate mixing column 26. To improve the mixing effect of the rotation, the reaction cup 11 is designed as a square reaction cup 11, and correspondingly, the reaction cup fixing groove 30 is also designed as a square. A second lifting mechanism 27 is provided between the C-shaped fixing plate 24 and the magnetic separation base plate assembly 12. The second lifting mechanism 27 includes a second fixed track 61, a second sliding track 62, and a second lifting motor 63. The second fixed track 61 is fixedly installed on the magnetic separation base plate assembly 12, and the second sliding track 62 is fixedly installed on the C-shaped fixing plate 24. The second sliding track 62 and the second fixed track 61 are in a limiting fit, and the second sliding track 62 can slide along the second fixed track 61. The second lifting motor 63 is fixedly installed below the second fixed track 61, and a second lifting worm gear 49 is provided at the second sliding track 62, which is connected to the second lifting motor 63. Driven by the lifting motor 263, the C-shaped fixing plate 24 can be raised and lowered, and the three cleaning fluid mixing columns 25 and one substrate mixing column 26 can be raised and lowered synchronously.

[0047] During operation, a reaction cup 11 is placed into the reaction cup placement slot 30 of the rotating disk 18 through the cup changing port 35. Then, the rotating disk 18 rotates one station, and the cleaning fluid injection needle 36 injects cleaning fluid into the reaction cup 11. Further, the rotating disk 18 rotates one station at a time, with reaction cups 11 being placed onto it sequentially. The reaction cup 11 rotates clockwise around the rotating disk bearing 19 until it reaches the cleaning fluid suction needle assembly 39 of the first cleaning fluid mixing and capping assembly 37. At this time, the suction needle fixing plate 23 moves down, and the cleaning fluid suction needle 41 inserts into the reaction cup 11 to draw out the liquid inside. Further still, the rotating disk 18 rotates one station to reach the cleaning fluid injection needle assembly 40 of the first cleaning fluid mixing and capping assembly 37. At this time, the C-shaped fixing plate 23 moves upward, and the corresponding cleaning fluid mixing column 25 rises accordingly, lifting the reaction cup 11. The upper end of the reaction cup 11 presses against the lower end of the corresponding capping head 46, and the cleaning fluid injection needle 36 injects cleaning fluid into the reaction cup 11. The corresponding cleaning fluid mixing column 25 rotates synchronously. After mixing is completed, the C-shaped fixing plate 23 moves downward, the reaction cup 11 returns to its original position, and moves with the rotating disk 18 to the second cleaning fluid mixing capping assembly 37. After the second cleaning fluid mixing capping assembly 37 completes the same operation, the reaction cup 11 moves with the rotating disk 18 to the third cleaning fluid mixing capping assembly 37. After the third cleaning fluid mixing capping assembly 37 completes the same operation, the reaction cup 11 moves with the rotating disk 18 to the substrate mixing capping assembly 38. At this time, the suction needle fixing plate 23 moves downward, and the substrate suction needle 52 is inserted into the corresponding reaction cup 11 to suction the liquid. After suction is completed, the suction needle fixing plate 23 moves upward. Further, the reaction cup 11 moves one position with the rotating disk 18. At this time, the C-shaped fixing plate 23 moves upward, and the corresponding substrate mixing column 26 rises, lifting the reaction cup 11. The upper end of the reaction cup 11 presses against the lower end of the corresponding capping head 46. The substrate mixing column 26 rotates to mix the substance in the reaction cup 11. After mixing, the C-shaped fixing plate 23 moves downward and resets. Finally, the reaction cup 11 moves with the rotating disk 18 to the cup changing port 35 and is removed by the arm assembly module 7. At the same time, another arm assembly module 7 inserts a new reaction cup 11.

[0048] Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the present invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A magnetic separation module, characterized in that: The system includes a magnetic separation base plate assembly, wherein a magnetic separation pot assembly and a magnetic separation liquid suction needle assembly are provided on the top surface of the magnetic separation base plate assembly, and a magnetic separation mixing assembly is provided on the bottom surface of the magnetic separation base plate assembly. The magnetic separation pot assembly includes a magnetic separation base, a magnetic separation disc cover, a rotating disc, a rotating disc bearing, a main drive pulley, and a main drive motor. The magnetic separation base is fixed to the upper surface of the magnetic separation base plate assembly. A rotating disc bearing is located at the magnetic separation base; the upper end of the rotating disc bearing is fixedly connected to the rotating disc, and the lower end of the rotating disc bearing is fixedly connected to the main drive pulley. The main drive motor is fixed to the lower surface of the magnetic separation base plate assembly, and the main drive motor and the main drive pulley are connected in a driving connection. The rotating disc has several reaction cup placement slots evenly distributed in a circular array. The tank contains a reaction cup; the magnetic separation disk cover has a cup changing port, a cleaning fluid injection needle, several cleaning fluid mixing capping assemblies and a substrate mixing capping assembly arranged in a circular array; the cleaning fluid mixing capping assembly includes a cleaning fluid suction needle assembly and a cleaning fluid injection needle assembly, and the substrate mixing capping assembly includes a substrate suction needle assembly and a substrate mixing capping assembly; as the rotating disk rotates through several stations, the cup changing port, the cleaning fluid injection needle, the cleaning fluid suction needle assembly, the cleaning fluid injection needle assembly, the substrate suction needle assembly and the substrate mixing capping assembly are always located directly above one of the reaction cup placement tanks; The magnetic separation liquid suction needle assembly includes a lifting mechanism and a liquid suction needle fixing plate. The lifting mechanism is provided between the magnetic separation base plate assembly and the liquid suction needle fixing plate. The liquid suction needle fixing plate is located directly above the magnetic separation pot assembly. The cleaning fluid liquid suction needle assembly includes a cleaning fluid liquid suction needle, and the substrate liquid suction needle assembly includes a substrate liquid suction needle. The upper half of the cleaning fluid liquid suction needle and the upper half of the substrate liquid suction needle are both fixed to the liquid suction needle fixing plate. The magnetic separation and mixing assembly includes a C-shaped fixing plate, several cleaning fluid mixing columns, a substrate mixing column, a second elevator, a cleaning fluid mixing motor, and a substrate mixing motor. The several cleaning fluid mixing columns and substrate mixing columns are arranged sequentially at the C-shaped fixing plate. The several cleaning fluid mixing columns and several cleaning fluid injection needle assemblies correspond one-to-one and are located on the same vertical axis. The substrate mixing columns and the substrate mixing cap are located on the same vertical axis. A cleaning fluid mixing synchronization belt is provided between the cleaning fluid mixing motor and the several cleaning fluid mixing columns, and a substrate mixing synchronization belt is provided between the substrate mixing motor and the substrate mixing columns. A second elevator is provided between the C-shaped fixing plate and the magnetic separation base plate assembly.

2. A magnetic separation module according to claim 1, characterized in that: The cleaning fluid suction needle assembly includes a cleaning fluid suction needle, the upper half of which is fixed to a suction needle fixing plate, and the lower half of which can penetrate into the magnetic separation pot assembly. After the rotating disk rotates through several stations, the lower end of the cleaning fluid suction needle is always aligned with one of the reaction cup placement slots.

3. A magnetic separation module according to claim 1, characterized in that: The cleaning fluid injection needle assembly includes a cleaning fluid injection needle, a mounting base, a bearing, a rotating hollow shaft, a return spring, and a capping head. The mounting base houses the bearing, the lower end of which is connected to the rotating hollow shaft. The lower end of the rotating hollow shaft is nested within the capping head. The rotating hollow shaft contains a return spring, with its two ends abutting against the inner wall of the rotating hollow shaft and the top of the capping head, respectively. The cleaning fluid injection needle passes sequentially through the bearing and the rotating hollow shaft, with its end connected to the capping head. The outer wall of the capping head is equipped with a guide pin, and correspondingly, the side wall of the rotating hollow shaft has a guide groove. As the rotating disk rotates through several stations, the lower end of the capping head always aligns with one of the reaction cup placement slots.

4. A magnetic separation module according to claim 1, characterized in that: The substrate aspiration needle assembly includes a substrate aspiration needle and a stabilizing base. The upper half of the substrate aspiration needle is fixed to the aspiration needle fixing plate, and the lower half of the substrate aspiration needle passes through the stabilizing base. After the rotating disk rotates for several positions, the lower end of the substrate aspiration needle is always aligned with one of the reaction cup placement slots.

5. A magnetic separation module according to claim 1, characterized in that: The substrate mixing cap includes a bearing, a capping head, and a return spring. The bearing is mounted on the magnetic separation disc cover, the capping head passes through the bearing, and a return spring is provided between the bearing and the capping head. A guide pin is provided at the bearing, and correspondingly, a guide groove is provided at the capping head.

6. A magnetic separation module according to claim 1, characterized in that: The lifting platform includes a fixed rail, a sliding rail, and a lifting motor. The fixed rail is fixedly mounted on the magnetic separation base plate assembly, and the lifting motor is fixedly mounted below the fixed rail. The sliding rail is in a limiting fit with the fixed rail and can slide up and down along the fixed rail. A suction needle fixing plate is fixedly connected to the upper end of the sliding rail, and a lifting worm gear is provided at the lower end of the sliding rail. The lifting worm gear is connected to the lifting motor.

7. A magnetic separation module according to claim 1, characterized in that: The second lifting platform includes a second fixed track, a second sliding track, and a second lifting motor. The second fixed track is fixedly mounted on the magnetic separation base plate assembly, and the second sliding track is fixedly mounted on the C-shaped fixed plate. The second sliding track and the second fixed track are in a limiting fit, and the second sliding track can slide along the second fixed track. The second lifting motor is fixedly mounted below the second fixed track, and the second lifting worm gear is provided at the second sliding track. The second lifting worm gear is connected to the second lifting motor.

8. A magnetic separation module according to claim 1, characterized in that: The cleaning fluid mixing column is rotatably connected to a C-shaped fixing plate. The top of the cleaning fluid mixing column is provided with a reaction cup fixing groove. The bottom of the cleaning fluid mixing column is provided with a cleaning fluid mixing driven wheel. A cleaning fluid mixing synchronous belt is provided between the cleaning fluid mixing motor and several cleaning fluid mixing driven wheels. Several cleaning fluid mixing tensioning wheels are provided on the bottom surface of the C-shaped fixing plate. The several cleaning fluid mixing tensioning wheels are connected to the cleaning fluid mixing synchronous belt.

9. A magnetic separation module according to claim 1, characterized in that: The substrate mixing column is rotatably connected to a C-shaped fixing plate. The top of the substrate mixing column is provided with a reaction cup fixing groove. The bottom of the substrate mixing column is provided with a substrate mixing driven wheel. A substrate mixing synchronous belt is provided between the substrate mixing motor and several substrate mixing driven wheels. Several substrate mixing tensioning wheels are provided on the lower surface of the C-shaped fixing plate. The several substrate mixing tensioning wheels are connected to the substrate mixing synchronous belt.

10. A high-speed, fully automated chemiluminescence immunoassay analyzer, characterized in that: Includes the magnetic separation module as described in any one of claims 1-9.

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