Salmon TVB-N value determination kit
The reagent kit, with its modular partitioning and buffer design, solves the problems of small storage capacity and poor stability of existing reagent kits, achieving efficient and accurate detection of salmon freshness while being both protective and economical.
Patent Information
- Application Number
- CN202520417386.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-11
- Publication Date
- 2026-01-27
- Estimated Expiration
- 2035-03-11
AI Technical Summary
Existing salmon quality testing kits have small storage capacity, poor sample stability, are not suitable for large-scale and real-time testing, are easily damaged by vibration, and have poor applicability.
A reagent kit with a protective casing was designed, which includes modular partitions and a buffer structure. It has a sample tube area, a reagent bottle area, a test strip area, and a pipette tip area. It uses snap-fit connections, sealing rings, and sponge cushioning to ensure the stability and independence of reagents and samples.
The improved protective and sealing properties of the reagent kit reduce the risk of damage from vibration, enabling efficient and accurate detection of salmon freshness, and possessing scalability, cost-effectiveness, and environmental friendliness.
Smart Images
Figure CN223836119U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to salmon quality testing, specifically to a salmon TVB-N value determination kit. Background Technology
[0002] Salmon, scientifically known as Atlantic salmon, is not consumed immediately after being caught but is transported chilled. Due to its rich nutritional content, it is highly susceptible to spoilage during transportation and storage, thus affecting its quality. Therefore, the ability to detect changes in the freshness of salmon during storage is of great significance.
[0003] Volatile basic nitrogen (TVB-N) is one of the indicators for measuring the freshness of salmon. It is related to the degradation of fish protein by exogenous spoilage microorganisms and their secreted enzymes. The higher the TVB-N content, the higher the degree of protein damage during storage and the lower the freshness of the salmon.
[0004] Existing fish quality-related reagent kits are limited, and most are simply small boxes. This setup has poor storage capacity and sample stability, with sample tubes easily broken by impact. It is inconvenient for situations requiring large-volume, real-time detection of salmon freshness, and has poor applicability. Utility Model Content
[0005] This utility model provides a salmon TVB-N value determination kit. The kit is equipped with a protective shell, which consists of a box body and a protective flip cover. The rear side of the protective flip cover is rotatably installed on the rear side of the top of the box body. The front side of the protective flip cover is connected to the front side of the top of the box body by a snap fastener. A handle is fixed on the top surface of the protective flip cover. A pipette can be detachably installed on the inside of the protective flip cover.
[0006] The box contains a sample tube area, a reagent bottle area, a test strip area, and a pipette tip area.
[0007] The sample tube area is provided with several test tube slots, and sample test tubes are inserted into each of the test tube slots;
[0008] The reagent bottle placement area is provided with a reagent bottle slot, in which a reagent tube is inserted and contains a reaction reagent;
[0009] The test strip placement area is used to place reaction test strips;
[0010] The pipette tip placement area is used to place the pipette tip, which is detachably connected to the pipette.
[0011] Furthermore, a horizontal fixing plate and a sponge filling the space below the horizontal fixing plate are fixedly installed inside the box. The horizontal fixing plate is provided with the sample tube area, the reagent bottle placement area, the test paper placement area and the pipette tip placement area.
[0012] The fixing plate and the sponge located in the sample tube area have a plurality of test tube slots that overlap vertically.
[0013] The fixing plate and the sponge located in the reagent bottle placement area have reagent bottle slots that overlap in the vertical direction.
[0014] Furthermore, the horizontal fixing plate is provided with a rectangular shallow groove whose length and width are both larger than the reaction test paper as the test paper placement area;
[0015] The pipette tip placement area is provided with a partition plate for separating the pipette tips, and the partition plate has multiple pipette tip slots.
[0016] Furthermore, a shielding sheet for covering the opening of the sample tube is connected to one side of the top of the sample tube, and the reagent tube is equipped with a rubber stopper.
[0017] Furthermore, a cloth bag is fixed to one side of the outer wall of the shell, and the cloth bag is used to store the reaction test paper.
[0018] Furthermore, the inner side of the protective flip cover is provided with a pipette strap for mounting a pipette.
[0019] Furthermore, a sealing ring is installed on the contact surface between the protective flip cover and the box body.
[0020] The advantages of this utility model are:
[0021] The advantages of this novel salmon TVB-N value determination kit are summarized as follows:
[0022] 1. High protection and sealing performance
[0023] Protective housing design: The box body and the protective flip cover are connected by snaps, with an internal sealing ring to form a sealed space, effectively waterproofing and isolating external contaminants, ensuring the stability of reagents and samples.
[0024] Shockproof and buffer structure: Rubber pads are provided at the four corners of the bottom of the box, combined with sponge filling under the horizontal fixing plate. The double buffer reduces the vibration and impact during transportation or operation, and significantly reduces the risk of sample tube breakage.
[0025] 2. Modular partitioning and efficient management
[0026] The functional areas are clearly defined: the box is divided into sample tube area, reagent bottle area, test strip area and pipette tip area. Each area is independent and does not interfere with each other, avoiding cross-contamination.
[0027] Pipette tip holder and divider: The pipette tip is fixed by a divider with a holder, ensuring that the tip is stored independently and easily accessible; the test strip placement area adopts a rectangular shallow groove design to prevent the test strip from contaminating other areas after it gets wet.
[0028] 3. Ease of operation and standardized procedures
[0029] Integrated tool design: The protective flip-top has a pipette strap inside for secure storage of the pipette, and the pipette tip is designed for easy access, reducing operation steps and improving testing efficiency.
[0030] Shielding film and automatic sealing: A flexible shielding film is connected to the top of the sample tube. During operation, the film can be opened to insert the sample and automatically closes after releasing the film, simplifying the sealing process and reducing the reaction between the sample solution and air.
[0031] 4. Detection reliability and accuracy
[0032] Reagent contamination prevention design: The reagent tubes are sealed with rubber stoppers, and the reaction test strips are stored in a moisture-proof cloth bag (with built-in snap-lock protective flap), ensuring the stability of the reagent and test strip activity and high consistency of colorimetric reaction.
[0033] 5. Scalability and Applicability
[0034] Large-capacity storage: The sample tube area supports 20 test tubes (arranged in a 5×4 matrix), and the reagent bottle slot can be expanded to accommodate multiple sizes to meet batch testing needs.
[0035] 6. Economic and environmental benefits
[0036] Replaceable consumable design: consumables such as pipette tips and test strips are individually packaged and taken as needed, reducing waste; waste consumables can be centrally processed through biosafety collection ports, meeting the requirements for harmlessness.
[0037] In summary, this invention solves the problems of easy contamination, cumbersome operation, and poor data stability of existing reagent kits through protective structural design and modular functional partitioning. While improving detection efficiency and accuracy, it also takes into account safety, economy, and environmental friendliness, making it suitable for salmon quality testing applications. Attached Figure Description
[0038] To more clearly illustrate the technical solutions in the embodiments of this utility model, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0039] Figure 1 This is an external view of a salmon TVB-N value determination kit according to the present invention;
[0040] Figure 2 This is a schematic diagram of the assay kit after it has been opened.
[0041] Figure 3 This is an internal cross-sectional view of the assay kit of this utility model. Detailed Implementation
[0042] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to those skilled in the art that the present invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described in order to avoid confusion with the present invention.
[0043] To fully understand this utility model, detailed steps and structures will be presented in the following description to illustrate the technical solution of this utility model. Preferred embodiments of this utility model are described in detail below; however, in addition to these detailed descriptions, this utility model may have other embodiments.
[0044] Reference Figure 1-3 As shown, this utility model provides a salmon TVB-N value determination kit. The kit includes a protective housing, which consists of a box body 1 and a protective flip cover 2. The rear of the protective flip cover 2 is rotatably mounted on the rear top of the box body 1, and the front of the protective flip cover 2 is connected to the front top of the box body 1 via a snap-fit 5. The protective housing is opened or closed by flipping the protective flip cover 2. A handle 3 is fixed to the top surface of the protective flip cover 2 for easy carrying and movement. A sealing ring 12 is installed on the contact surface between the protective flip cover 2 and the box body 1 to ensure the airtightness of the protective housing.
[0045] Rubber pads are fixed at the four corners of the bottom surface of the box 1. The rubber pads increase the friction between the reagent kit and the placement surface and have a certain shock absorption and cushioning effect, so as to prevent the internal sample tubes from being damaged by bumps during the placement or movement of the reagent kit, and further improve the practical performance of the reagent kit.
[0046] In an optional embodiment, a pipette strap 11 is provided on the inner side of the protective flip cover 2 to fix the pipette inside the protective housing.
[0047] A horizontal fixing plate 6 and a sponge 16 filling the space below the horizontal fixing plate 6 are fixedly installed inside the box 1. The horizontal fixing plate 6 is provided with a sample tube area, a reagent bottle placement area, a test paper placement area 7 and a pipette tip placement area 13.
[0048] The horizontal fixing plate 6 and sponge 16 in the sample tube area have 20 vertically overlapping 5*4 slots for each sample tube. A sample tube 8 is inserted into each slot. Similarly, the horizontal fixing plate 6 and sponge 16 in the reagent bottle placement area have vertically overlapping reagent bottle slots. A reagent tube 10 containing a reaction reagent is inserted into each reagent bottle slot. This reagent will develop a color upon contact with the reaction paper, facilitating observation. In one embodiment, the reagent can be a 1% boric acid absorbent solution or trichloroacetic acid (TCA).
[0049] In an optional embodiment, a shielding film 9 is connected to one side of the top of the sample tube 8. The shielding film 9 can cover the opening of the sample tube 8, providing a simple seal. In use, the shielding film 9 can be removed and the test sample can be placed into the sample tube 8. The reagent tube 10 is equipped with a rubber stopper to prevent the reaction reagent from flowing out or evaporating.
[0050] In an optional embodiment, the horizontal fixing plate 6 has a rectangular shallow groove, the length and width of which are larger than the reaction test strip, as a test strip placement area 7 to prevent the test strip from contaminating other areas after it becomes wet. The pipette tip placement area 13 is provided with a partition plate for separating the pipette tips. The partition plate has multiple tip slots, which can fix multiple pipette tips in place and prevent them from falling off.
[0051] In an optional embodiment, a cloth bag 4 is fixed to one outer side wall of the housing. The cloth bag 4 is used to store the reaction test strips. The cloth bag 4 includes a bag body and a protective flap, which are connected by snaps to prevent the reaction test strips from falling out.
[0052] The working principle of this utility model is as follows:
[0053] 1) In this embodiment, the operator first needs to release the latch 5 to open the protective shell. Then, the shielding film 9 is removed, and sterile forceps are used to place the pre-treated salmon sample into the sample tube 8. Depending on the actual needs, multiple salmon samples can be placed into different sample tubes 8 for subsequent processing and analysis. It is recommended to process 6 samples in parallel per batch to improve detection accuracy.
[0054] 2) Next, remove the pipette from the inside of the protective flip cover 2, and take a pipette tip from the pipette tip placement area 13, attaching it to the pipette. Then, open the rubber stopper of the reagent tube 10, accurately pipette a certain amount of liquid reaction reagent using the reverse pipetting method, then remove the shielding film 9 and tilt the pipette tip against the inner wall of the sample tube 8, slowly releasing the reaction reagent into the sample tube to avoid air bubbles. After releasing, the shielding film 9 will automatically close to ensure a seal. Gently shake the protective shell to ensure that the reaction reagent and salmon sample are fully mixed and reacted to form a relatively homogeneous sample solution.
[0055] 3) Next, take a reaction test strip and place it in the test strip placement area 7. Then, replace it with a new pipette tip and install it on the pipette to prepare for the next step.
[0056] 4) Next, use a pipette to draw the sample solution from sample tube 8 and drop it onto the center of the reaction paper. Let it stand for a period of time to allow the reaction to proceed. By observing the degree of color development on the test paper, the TVB-N value of the salmon sample can be measured, thereby assessing the freshness and quality of the salmon.
[0057] The preferred embodiments of this utility model have been described above. It should be understood that this utility model is not limited to the specific embodiments described above. Devices and structures not described in detail herein should be understood as being implemented in a conventional manner within the art. Any person skilled in the art can make many possible variations and modifications to the technical solutions of this utility model using the disclosed methods and techniques, or modify them into equivalent embodiments with equivalent changes, without departing from the scope of the technical solution of this utility model. This does not affect the essential content of this utility model. Therefore, any simple modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of this utility model, without departing from the content of the technical solution of this utility model, still fall within the protection scope of the technical solution of this utility model.
Claims
1. A salmon TVB-N value determination kit, the kit having a protective shell, the protective shell being composed of a box body (1) and a protective flip cover (2), the rear side of the protective flip cover (2) being rotatably mounted on the top rear side of the box body (1), the front side of the protective flip cover (2) being connected to the top front side of the box body (1) by a snap fastener (5), and a handle (3) being fixed on the top surface of the protective flip cover (2), characterized in that, A pipette is detachably mounted on the inner side of the protective flip cover (2); The box (1) is provided with a sample tube area, a reagent bottle area, a test paper area (7) and a pipette tip area (13); The sample tube area is provided with several test tube slots, and sample tubes (8) are inserted into each of the test tube slots; The reagent bottle placement area is provided with a reagent bottle slot, and a reagent tube (10) is inserted into the reagent bottle slot. The reagent tube (10) contains a reaction reagent. The test paper placement area (7) is used to place the reaction test paper; The pipette tip placement area (13) is used to place the pipette tip, which is detachably connected to the pipette.
2. The salmon TVB-N value determination kit as described in claim 1, characterized in that, A horizontal fixing plate (6) and a sponge (16) filling the space below the horizontal fixing plate (6) are fixedly installed inside the box body (1). The horizontal fixing plate (6) is provided with the sample tube area, the reagent bottle placement area, the test paper placement area (7) and the pipette tip placement area (13). The fixing plate and the sponge (16) located in the sample tube area have a plurality of test tube slots that overlap vertically. The fixing plate and the sponge (16) located in the reagent bottle placement area have reagent bottle slots that overlap in the vertical direction.
3. The salmon TVB-N value determination kit as described in claim 2, characterized in that, The horizontal fixing plate (6) has a rectangular shallow groove with a length and width greater than the reaction test paper as the test paper placement area (7); The pipette tip placement area (13) is provided with a partition plate for separating the pipette tips, and the partition plate is provided with multiple pipette tip slots.
4. The salmon TVB-N value determination kit as described in claim 2, characterized in that, The sample tube (8) is connected to a shielding film (9) on one side of its top for covering the opening of the sample tube (8), and the reagent tube (10) is equipped with a rubber stopper.
5. The salmon TVB-N value determination kit as described in claim 1, characterized in that, A cloth bag (4) is fixed to one side of the outer wall of the shell, and the cloth bag (4) is used to store the reaction test paper.
6. The salmon TVB-N value determination kit as described in claim 1, characterized in that, The inner side of the protective flip cover (2) is provided with a pipette strap (11) for mounting a pipette.
7. The salmon TVB-N value determination kit as described in claim 1, characterized in that, A sealing ring (12) is installed on the contact end face between the protective flip cover (2) and the box body (1).