Single cell sorting fixing device
By employing a structural design that combines cylinders and motors in the single-cell sorting and fixation device, precise positioning and stable fixation of the biological culture chip are achieved, solving the problem of inaccurate cell positioning caused by insecure fixation or difficulty in adjustment, and improving the accuracy of sorting and the reliability of experimental results.
Patent Information
- Application Number
- CN202520349047.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-02-28
- Publication Date
- 2026-02-10
- Estimated Expiration
- 2035-02-28
AI Technical Summary
Existing single-cell sorting and fixation devices often result in inaccurate cell placement due to insecure fixation or difficulty in adjustment, affecting the reliability of experimental results and potentially altering the physiological state of the cells.
The structure design includes components such as a base, support, slide bar, slider, cylinder, motor and screw. The cylinder and motor work together to achieve precise positioning and stable fixation of the biological culture chip, ensuring that the cells do not shift or fall off during the sorting process.
This improves the accuracy and efficiency of single-cell sorting, ensuring the reliability of experimental results and the stability of cell physiological state.
Smart Images
Figure CN223892728U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of cell sorting technology, specifically a single-cell sorting and fixing device. Background Technology
[0002] Single-cell sorting is a technique for isolating individual cells from a cell population, with the aim of analyzing or culturing each cell separately to understand cellular heterogeneity. Single-cell sorting is an important tool for efficiently screening and classifying complex biological samples at the cellular level.
[0003] The principles of single-cell sorting and immobilization devices are diverse, including laser detection and charge separation in flow cytometers, microchannel control and droplet encapsulation in microfluidic chips, and scaffolding devices in oral pipettes that provide stability.
[0004] In existing technologies, single-cell sorting and fixation devices often result in inaccurate cell placement due to insecure fixation or difficulty in adjustment. This not only affects the reliability of experimental results but may also alter the physiological state of cells, thereby affecting the effectiveness of subsequent experiments. Therefore, a single-cell sorting and fixation device is proposed to address the above problems. Summary of the Invention
[0005] To address the shortcomings of existing technologies, in the current single-cell sorting and fixation devices, the cell positions are often inaccurate due to insecure fixation or difficulty in adjustment. This not only affects the reliability of experimental results but may also cause changes in the physiological state of cells, thereby affecting the effectiveness of subsequent experiments. This invention proposes a single-cell sorting and fixation device.
[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: A single-cell sorting and fixing device of this utility model includes a base; a bracket is fixedly installed on one side of the upper part of the base, and a mounting seat is fixedly installed at one end of the upper part of the bracket. Sliding holes are opened on both sides of the top of the mounting seat, and a sliding rod is slidably installed in the sliding holes. A fixing block is fixedly installed at the lower end of the sliding rod, and a sorting instrument is fixedly installed on one side of the fixing block. A support frame is fixedly installed on the upper part of the base, and a sliding platform is slidably arranged on the upper part of the support frame. Sliding blocks are fixedly installed on both sides of the bottom of the sliding platform. Holes are opened inside the sliding blocks, and a threaded groove is opened in the hole of one of the sliding blocks. The sliding blocks are slidably arranged within the support frame, and slide rails are fixedly installed on the upper parts of both ends of the sliding platform. The slide has a fixed platform on its upper part, and a mounting groove is provided at the bottom of the fixed platform. A toothed plate is fixedly installed on one side of the center of the mounting groove. The fixed platform is slidably mounted on a slide rail. A sliding groove is provided in the upper part of the fixed platform, and mounting blocks are slidably installed in both ends of the sliding groove. Each mounting block has a threaded hole. A clamping plate is fixedly installed on the upper part of the mounting block. A second cylinder is fixedly installed on both sides of the upper part of the fixed platform. A positioning plate is fixedly installed on the piston rod of each of the second cylinders. A biological culture chip is fixed in the center of the upper part of the fixed platform through the clamping plate and the positioning plate. Through the cooperation of the clamping plate and the positioning plate, this invention can firmly fix the biological culture chip, prevent the cells from shifting or falling off during the sorting process, and thus improve the reliability of the experimental results.
[0007] Preferably, a first cylinder is fixedly installed on the top of the mounting base, and the piston rod of the first cylinder is fixedly connected to the top of the fixing block. By extending and retracting the first cylinder, the height of the fixing block and the sorting instrument can be easily adjusted, thereby achieving precise control of the position of the sorting instrument.
[0008] Preferably, a third motor is fixedly installed on one end of the fixed platform, and a bidirectional screw is rotatably installed in the sliding groove of the fixed platform. The mounting blocks are slidably installed on both ends of the bidirectional screw. The rotating shaft of the third motor is fixedly connected to one end of the bidirectional screw. By setting the third motor and the bidirectional screw to drive the slider to move the clamping plate synchronously, the distance between the two clamping plates is adjusted according to the length of the biological culture chip.
[0009] Preferably, a second motor is fixedly installed at the bottom of the slide table. The rotating shaft of the second motor passes through the upper part of the slide table and a gear is fixedly installed thereon. The teeth of the gear mesh with the tooth grooves on the gear plate. By driving the gear to mesh with the gear plate through the second motor, the slide table can move smoothly in the horizontal direction.
[0010] Preferably, a limiting rod and a lead screw are respectively provided on the lower sides of the slide table. The limiting rod is fixedly installed in the support frame, and both ends of the lead screw are rotatably installed in the support frame through bearings. The slider is slidably installed on the limiting rod and the lead screw respectively. By setting the limiting rod and the lead screw, not only is the stability of the slide table movement improved, but also the deviation or shaking of the slider during the movement is prevented, further ensuring the reliability of the experimental results.
[0011] Preferably, a first motor is fixedly installed on one end of the support frame, and the rotating shaft of the first motor is fixedly connected to one end of the lead screw. The first motor drives the lead screw to rotate, thereby realizing the precise adjustment of the slider and the components installed on the slider.
[0012] The advantages of this utility model are:
[0013] By using a single-cell sorting and fixation device for cell sorting, the biological culture chip is placed on a fixed stage. Simultaneously, a third motor and a second cylinder are activated. The third motor's rotating shaft drives a bidirectional screw to rotate, which in turn moves a clamping plate via a slider, positioning and clamping both ends of the biological culture chip. Simultaneously, the piston rod of the second cylinder extends and retracts, moving a positioning plate to further position and clamp the sides of the biological culture chip. The first cylinder is then activated, causing the sorting device to descend and inject the sorted single cells into the culture tank of the biological culture chip. The second motor then drives a gear to rotate, which, through the interaction of the gear and gear plate, moves the fixed stage laterally, adjusting the lateral position of the biological culture chip. The first motor then drives a lead screw to rotate, which, through a limit rod and slider, moves the slide stage longitudinally, simultaneously moving the biological culture chip longitudinally. This structural design, which adjusts the longitudinal position, achieves precise positioning and stable fixation. It solves the problem in existing single-cell sorting and fixation devices where inaccurate cell positioning is often caused by insecure fixation or difficulty in adjustment. This not only affects the reliability of experimental results but may also alter the physiological state of the cells, thus affecting the effectiveness of subsequent experiments. This design improves the accuracy and efficiency of single-cell sorting. Attached Figure Description
[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0015] Figure 1 This is a schematic diagram of one side of the single-cell sorting and immobilization device.
[0016] Figure 2 This is a schematic diagram of the other side of the single-cell sorting and immobilization device.
[0017] Figure 3 This is a schematic diagram of the positioning mechanism.
[0018] Figure 4 This is a schematic diagram of the longitudinal adjustment mechanism.
[0019] Figure 5 This is a schematic diagram of the lateral adjustment mechanism.
[0020] In the diagram: 1. Base; 2. Bracket; 3. Mounting seat; 4. Slide rod; 5. Fixing block; 6. Sorter; 7. First cylinder; 8. Support frame; 9. Slide table; 10. Limiting rod; 11. Lead screw; 12. First motor; 13. Slider; 14. Second motor; 15. Gear; 16. Slide rail; 17. Fixing table; 18. Mounting block; 19. Clamping plate; 20. Bidirectional screw; 21. Third motor; 22. Second cylinder; 23. Positioning plate; 24. Biological culture chip; 25. Toothed plate. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those skilled in the art without creative effort are within the protection scope of the present utility model.
[0022] Please see Figure 1-5As shown, a single-cell sorting and fixing device includes a base 1; a bracket 2 is fixedly installed on one side of the upper part of the base 1, and a mounting base 3 is fixedly installed on one end of the upper part of the bracket 2. Sliding holes are provided on both sides of the top of the mounting base 3, and a sliding rod 4 is slidably installed in the sliding holes. A fixing block 5 is fixedly installed at the lower end of the sliding rod 4, and a sorting instrument 6 is fixedly installed on one side of the fixing block 5. A support frame 8 is fixedly installed on the upper part of the base 1, and a sliding platform 9 is slidably arranged on the upper part of the support frame 8. Slider blocks 13 are fixedly installed on both sides of the bottom of the sliding platform 9. Holes are provided inside each slider 13, and a threaded groove is provided in one of the holes of the slider 13. Block 13 is slidably disposed within the support frame 8. Slide rails 16 are fixedly installed on the upper parts of both ends of the slide table 9. A fixed platform 17 is provided on the upper part of the slide table 9. An installation groove is opened at the bottom of the fixed platform 17, and a toothed plate 25 is fixedly installed on one side of the center of the installation groove. The fixed platform 17 is slidably mounted on the slide rails 16. A sliding groove is opened in the upper part of the fixed platform 17, and mounting blocks 18 are slidably installed in both ends of the sliding groove. Threaded holes are opened in each mounting block 18. A clamping plate 19 is fixedly installed on the upper part of the mounting block 18. Second cylinders 22 are fixedly installed on both sides of the upper part of the fixed platform 17. A [missing information - likely a device or component] is fixedly installed on the piston rod of each of the second cylinders 22. Positioning plate 23, the fixed platform 17 has a biological culture chip 24 fixed to the center of the upper part of the positioning plate 23 via clamping plate 19; during operation, when using the single-cell sorting and fixing device for cell sorting, the biological culture chip 24 is placed on the fixed platform 17, and the third motor 21 and the second cylinder 22 are started at the same time. The rotating shaft of the third motor 21 drives the bidirectional screw 20 to rotate, and the bidirectional screw 20 drives the clamping plate 19 to move relative to each other through the slider 13, positioning and clamping the two ends of the biological culture chip 24. At the same time, the piston rod of the second cylinder 22 extends and retracts, driving the positioning plate 23 to position and clamp the two sides of the biological culture chip 24. The first cylinder 7 is started to drive the separation. The sorting instrument 6 descends, injecting the sorted single cells into the culture tank of the biological culture chip 24. Then, the second motor 14 is started, driving the gear 15 to rotate. Through the interaction between the gear 15 and the toothed plate 25, the fixed stage 17 moves laterally along the slide rail 16 on the upper part of the slide table 9, adjusting the lateral position of the biological culture chip 24. The first motor 12 is started, driving the lead screw 11 to rotate. The lead screw 11 drives the slider 13 through the limit rod 10, and the slide table 9 moves longitudinally within the support frame 8. The slide table 9 moves longitudinally through the fixed stage 17, adjusting its longitudinal position, thus achieving precise fixation and position adjustment of the biological culture chip 24.
[0023] The mounting base 3 is fixedly mounted on the top of a first cylinder 7, and the piston rod of the first cylinder 7 is fixedly connected to the top of the fixing block 5. During operation, when using the single-cell sorting and fixing device to sort cells, the first cylinder 7 will extend and retract its piston rod as needed, thereby driving the fixing block 5 and the sorting instrument 6 on it to move up and down. This vertical adjustment ensures that the sorting instrument 6 can accurately align with the biological culture chip 24 at different heights, thus improving the accuracy of sorting.
[0024] A third motor 21 is fixedly installed on one end of the fixed platform 17. A bidirectional screw 20 is rotatably installed in the sliding groove of the fixed platform 17. The mounting blocks 18 are slidably installed on both ends of the bidirectional screw 20. The rotating shaft of the third motor 21 is fixedly connected to one end of the bidirectional screw 20. During operation, when using the single-cell sorting and fixing device for cell sorting, the third motor 21 drives the bidirectional screw 20 to rotate. Since there is a threaded fit between the mounting block 18 and the bidirectional screw 20, the mounting block 18 will move along the axial direction of the bidirectional screw 20, so that the distance between the two clamping plates 19 can be flexibly adjusted to adapt to biological culture chips 24 of different lengths, ensuring the stability and applicability of the clamping.
[0025] A second motor 14 is fixedly installed at the bottom of the slide table 9. The rotating shaft of the second motor 14 passes through the upper part of the slide table 9 and a gear 15 is fixedly installed thereon. The teeth on the gear 15 mesh with the tooth grooves on the tooth plate 25. During operation, when the single-cell sorting and fixing device is used for cell sorting, the second motor 14 drives the gear 15 to rotate. Due to the meshing relationship between the gear 15 and the tooth plate 25, the slide table 9 will move horizontally along the length of the tooth plate 25, realizing the precise adjustment of the slide table 9 in the horizontal direction and ensuring that the biological culture chip 24 can be moved to the optimal working position of the sorter 6.
[0026] Limiting rods 10 and lead screws 11 are respectively provided on the lower sides of the slide table 9. The limiting rods 10 are fixedly installed in the support frame 8, and both ends of the lead screw 11 are rotatably installed in the support frame 8 through bearings. The slider 13 is slidably installed on the limiting rods 10 and lead screws 11 respectively. During operation, when using the single-cell sorting and fixing device for cell sorting, the lead screw 11 rotates under the drive of the first motor 12. Since there is a threaded fit between the slider 13 and the lead screw 11, the slider 13 will move precisely along the axial direction of the lead screw 11. At the same time, the limiting rods 10 play a role in guiding and stabilizing the movement trajectory of the slider 13, realizing precise fine-tuning of the slide table 9 in the horizontal direction, and further improving the sorting accuracy.
[0027] A first motor 12 is fixedly installed on one end of the support frame 8. The rotating shaft of the first motor 12 is fixedly connected to one end of the lead screw 11. During operation, when using the single-cell sorting and fixing device for cell sorting, the first motor 12 serves as a power source to drive the lead screw 11 to rotate. The rotation of the lead screw 11 is converted into the linear motion of the slider 13, thereby driving the slide table 9 and the biological culture chip 24 on it to move horizontally. This not only simplifies the transmission mechanism but also improves the accuracy and stability of the transmission.
[0028] Working principle: When using the single-cell sorting and fixing device for cell sorting, the biological culture chip 24 is placed on the fixing stage 17. Simultaneously, the third motor 21 and the second cylinder 22 are activated. The rotating shaft of the third motor 21 drives the bidirectional screw 20 to rotate. The bidirectional screw 20, through the slider 13, drives the clamping plate 19 to move relative to the chip, positioning and clamping both ends of the biological culture chip 24. At the same time, the piston rod of the second cylinder 22 extends and retracts, driving the positioning plate 23 to position and clamp both sides of the biological culture chip 24. The first cylinder 7 is activated, causing the sorting instrument 6 to descend and inject the sorted single cells into the biological culture chip. Inside the culture tank of chip 24, the second motor 14 is started to drive the gear 15 to rotate. The gear 15 and the toothed plate 25 drive the fixed stage 17 to move laterally along the slide rail 16 on the upper part of the slide table 9, adjusting the lateral position of the biological culture chip 24. The first motor 12 is started to drive the lead screw 11 to rotate. The lead screw 11 drives the slider 13 to move longitudinally within the support frame 8 through the limit rod 10. The slide table 9 drives the biological culture chip 24 to move longitudinally through the fixed stage 17, adjusting its longitudinal position, thus realizing the precise fixation and position adjustment of the biological culture chip 24.
Claims
1. A single-cell sorting and immobilization device, characterized in that: Includes a base (1); a bracket (2) is fixedly installed on one side of the upper part of the base (1), and a mounting seat (3) is fixedly installed on one end of the upper part of the bracket (2). Sliding holes are provided on both sides of the top of the mounting seat (3), and a sliding rod (4) is slidably installed in the sliding hole. A fixing block (5) is fixedly installed at the lower end of the sliding rod (4), and a sorting instrument (6) is fixedly installed on one side of the fixing block (5). A support frame (8) is fixedly installed on the upper part of the base (1), and a sliding table (9) is slidably arranged on the upper part of the support frame (8). Sliders (13) are fixedly installed on both sides of the bottom of the sliding table (9). Holes are provided inside the sliders (13), and a threaded groove is provided in the hole of one of the sliders (13). The sliders (13) are slidably arranged in the support frame (8), and the upper parts of both ends of the sliding table (9) are fixed. A slide rail (16) is installed, and a fixed platform (17) is provided on the upper part of the slide table (9). The bottom of the fixed platform (17) is provided with an installation groove, and a toothed plate (25) is fixedly installed on one side of the center of the installation groove. The fixed platform (17) is slidably installed on the slide rail (16). A sliding groove is provided in the upper part of the fixed platform (17), and an installation block (18) is slidably installed in both ends of the sliding groove. A threaded hole is provided in each of the installation blocks (18). A clamping plate (19) is fixedly installed on the upper part of the installation block (18). A second cylinder (22) is fixedly installed on both sides of the upper part of the fixed platform (17). A positioning plate (23) is fixedly installed on the piston rod of the second cylinder (22). A biological culture chip (24) is fixedly fixed in the center of the upper part of the fixed platform (17) through the clamping plate (19) and the positioning plate (23).
2. The single-cell sorting and immobilization device according to claim 1, characterized in that: The first cylinder (7) is fixedly installed on the top of the mounting base (3), and the piston rod of the first cylinder (7) is fixedly connected to the top of the fixing block (5).
3. The single-cell sorting and immobilization device according to claim 1, characterized in that: A third motor (21) is fixedly installed on one end of the fixed platform (17). A bidirectional screw (20) is rotatably installed in the groove of the fixed platform (17). The mounting blocks (18) are slidably installed on both ends of the bidirectional screw (20). The rotating shaft of the third motor (21) is fixedly connected to one end of the bidirectional screw (20).
4. The single-cell sorting and immobilization device according to claim 1, characterized in that: The bottom of the slide (9) is fixedly installed with a second motor (14). The rotating shaft of the second motor (14) passes through the upper part of the slide (9) and is fixedly installed with a gear (15). The teeth on the gear (15) mesh with the tooth grooves on the gear plate (25).
5. The single-cell sorting and immobilization device according to claim 1, characterized in that: The slide (9) is provided with a limit rod (10) and a lead screw (11) on both sides below. The limit rod (10) is fixedly installed in the support frame (8). Both ends of the lead screw (11) are rotatably installed in the support frame (8) through bearings. The slider (13) is slidably installed on the limit rod (10) and the lead screw (11) respectively.
6. The single-cell sorting and immobilization device according to claim 1, characterized in that: A first motor (12) is fixedly installed on one end of the support frame (8), and the rotating shaft of the first motor (12) is fixedly connected to one end of the lead screw (11).