Single cell sterile printing and separating device
By designing a single-cell sterile printing and separation device, and utilizing the combination of a drive plate and a stirring rod, the problem of unevenness in cell suspension during the dilution process was solved, achieving high-precision and high-efficiency single-cell separation and printing.
Patent Information
- Application Number
- CN202520394504.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-07
- Publication Date
- 2026-02-10
- Estimated Expiration
- 2035-03-07
AI Technical Summary
Existing devices struggle to achieve high-precision, high-throughput single-cell separation and printing in sterile environments. Furthermore, cell suspensions are prone to sedimentation or aggregation during dilution, leading to uneven cell concentrations and impacting separation accuracy and efficiency.
A single-cell sterile printing separation device was designed, including a separation cup, a stirring rod, and a multi-well plate. The stirring rod is driven to rotate by the cooperation of the driving plate, the arc plate, and the driving rod to ensure uniform distribution of the cell suspension. The uniform dilution and separation of cells are achieved by the rotation of the baffle and the sealing gasket.
This ensures uniform distribution of the cell suspension during dilution, prevents cell aggregation or sedimentation, and improves the accuracy and efficiency of single-cell separation.
Smart Images
Figure CN223892732U_ABST
Abstract
Description
Technical Field
[0001] This invention belongs to the field of single-cell aseptic printing and separation technology, specifically a single-cell aseptic printing and separation device. Background Technology
[0002] Single-cell analysis can reveal cellular heterogeneity and provide key information for understanding cell function, disease mechanisms, and personalized treatment. However, single-cell manipulation technology faces many challenges, especially in achieving high-precision, high-throughput single-cell separation and printing in a sterile environment, thus requiring a sterile single-cell printing and separation device.
[0003] While traditional single-cell separation methods can achieve single-cell separation, they have shortcomings in terms of aseptic operation and high-precision printing. In the process of single-cell separation and dilution, the homogeneity of the cell suspension is crucial to the separation effect.
[0004] However, existing devices still have some problems in cell dilution and separation. When diluting cell suspensions, they usually rely on simple pipetting operations or static mixing, which makes it difficult to ensure that cells are evenly distributed in the suspension. Moreover, cells are prone to sedimentation or aggregation due to gravity, resulting in uneven cell concentration after dilution, which affects the accuracy and efficiency of single-cell separation. Therefore, a single-cell sterile printing separation device is proposed to address the above problems. Summary of the Invention
[0005] To overcome the shortcomings of existing technologies and address the problems of existing equipment, this utility model proposes a single-cell sterile printing and separation device.
[0006] The technical solution adopted by this utility model to solve its technical problem is a single-cell sterile printing and separation device, including: a separation cup, a transparent plate fixedly connected to one side of the separation cup, a top cover movably connected to the top of the separation cup, a support groove inside the top cover, a limiting groove on the top of the top cover, a base plate rotatably connected inside the support groove, a stirring rod fixedly connected to the lower surface of the base plate, the stirring rod rotatably connected to the inside of the top cover and extending into the inside of the separation cup, a sliding groove on the upper surface of the base plate, an anti-slip pad fixedly connected to the inner wall of the sliding groove, an arc-shaped plate fixedly connected to the upper surface of the base plate, a drive plate drivingly connected to one side of the arc-shaped plate, and a drive rod fixedly connected to the lower surface of one end of the drive plate. With the cooperation of the arc-shaped plate and the drive rod, a thrust is applied to the arc-shaped plate and the sliding groove, which drives the base plate and the stirring rod to rotate, thereby stirring the cell suspension to ensure uniform cell distribution.
[0007] Preferably, the drive rod is connected to the slide and the anti-slip pad in a transmission connection. A rotating rod is fixedly connected to the upper surface of the drive plate. The rotating rod is rotatably connected to the inside of the upper cover and extends to the top of the upper cover. The anti-slip pad can increase the friction with the surface of the drive rod, thereby enhancing the thrust on the slide.
[0008] Preferably, a connecting frame is fixedly connected to the rotating rod, and a limiting block is fixedly connected to the bottom of the connecting frame. The limiting block is slidably connected inside the limiting groove. A connecting shaft is fixedly connected to one side of the separating cup. The limiting block moves inside the limiting groove, and one end of the rotating rod is located inside the upper cover, while the other end is located outside the upper cover. This cooperates with the connecting frame to improve the fixing strength of the drive plate, thereby improving the pushing strength of the base plate.
[0009] Preferably, the separation cup has symmetrically formed fixing grooves inside, a baffle is rotatably connected to the connecting shaft, and a sealing gasket is fixedly connected to the outside of the baffle. The sealing gasket can increase the friction with the inner wall of the separation cup, thereby preventing leakage when the single-cell suspension is inside the separation cup.
[0010] Preferably, the baffle and the sealing gasket are both in contact with the inner wall of the separation cup, and a perforated plate is rotatably connected to the connecting shaft. A spring is fixedly connected to one side of both the baffle and the perforated plate. The perforated plate is located below the baffle. Rotating the baffle separates the two, allowing the stirred single-cell suspension to fall onto the perforated plate, thereby performing the single-cell separation operation.
[0011] Preferably, one end of the spring is fixedly connected to a positioning pad, and one side of each of the two sets of positioning pads is fixedly connected to one side of the baffle and the perforated plate, respectively. The two sets of positioning pads are movably connected inside the two sets of fixing grooves. By the elastic force of the spring itself, the two sets of positioning pads can be pushed into the fixing grooves, thereby fixing the baffle and the perforated plate, which facilitates subsequent use.
[0012] The advantages of this invention are as follows: the rotating rod rotates, causing the fan-shaped end of the drive plate to slide past the inside of the arc-shaped plate, while the other end drives the drive rod into the anti-slip pad in the chute. A thrust is applied to the arc-shaped plate and the chute respectively. With the cooperation of the drive plate, the arc-shaped plate, and the drive rod, the base plate rotates. One rotation of the rotating rod causes the base plate and the stirring rod to rotate one revolution, thereby stirring the cell suspension to ensure uniform cell distribution and prevent cell aggregation or sedimentation during dilution. This ensures the uniformity of the diluted cell concentration and avoids affecting the precision and efficiency of cell separation.
[0013] This invention allows the baffle and sealing gasket to rotate around the connecting shaft, causing the diluted cell suspension to fall onto the porous plate, thus enabling the separation of single cells. When the positioning pads on the baffle and the porous plate are located outside the two sets of fixing grooves, the springs themselves can push the two sets of positioning pads into the fixing grooves, thereby fixing the baffle and the porous plate and facilitating subsequent use. Attached Figure Description
[0014] To more clearly illustrate the technical solutions in the embodiments of this utility model or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of this utility model. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.
[0015] Figure 1 This is a schematic diagram of the overall mechanism;
[0016] Figure 2 This is a schematic diagram of the cross-section of the separation cup;
[0017] Figure 3 This is a schematic diagram of the stirring assembly;
[0018] Figure 4 This is a schematic diagram of a fixed component;
[0019] Figure 5 This is a schematic diagram of the material feeding assembly.
[0020] In the diagram: 1. Separating cup; 2. Transparent plate; 3. Top cover; 4. Support groove; 5. Limiting groove; 6. Base plate; 7. Stirring rod; 8. Slide groove; 9. Anti-slip pad; 10. Arc plate; 11. Drive plate; 12. Drive rod; 13. Rotating rod; 14. Connecting frame; 15. Limiting block; 16. Connecting shaft; 17. Fixing groove; 18. Baffle; 19. Sealing gasket; 20. Perforated plate; 21. Spring; 22. Positioning pad. Detailed Implementation
[0021] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present utility model.
[0022] Please see Figure 1-5As shown, a single-cell sterile printing separation device includes: a separation cup 1, a transparent plate 2 fixedly connected to one side of the separation cup 1, a top cover 3 movably connected to the top of the separation cup 1, a support groove 4 formed inside the top cover 3, a limiting groove 5 formed on the top of the top cover 3, a base plate 6 rotatably connected inside the support groove 4, a stirring rod 7 fixedly connected to the lower surface of the base plate 6, the stirring rod 7 rotatably connected inside the top cover 3 and extending into the interior of the separation cup 1, a sliding groove 8 formed on the upper surface of the base plate 6, an anti-slip pad 9 fixedly connected to the inner wall of the sliding groove 8, and a fixed... An arc-shaped plate 10 is connected to the drive plate 11 on one side of the arc-shaped plate 10. A drive rod 12 is fixedly connected to the lower surface of one end of the drive plate 11. The drive rod 12 is connected to the slide groove 8 and the anti-slip pad 9. A rotating rod 13 is fixedly connected to the upper surface of the drive plate 11. The rotating rod 13 is rotatably connected to the inside of the upper cover 3 and extends to the top of the upper cover 3. A connecting frame 14 is fixedly connected to the rotating rod 13. A limit block 15 is fixedly connected to the bottom of the connecting frame 14. The limit block 15 is slidably connected to the inside of the limit groove 5. A connecting shaft 16 is fixedly connected to one side of the separation cup 1.
[0023] Single-cell manipulation technology faces numerous challenges, especially achieving high-precision, high-throughput single-cell separation and printing in a sterile environment. Therefore, a sterile single-cell printing and separation device is needed. In practical use, a cell suspension is added to the separation cup 1, and then the top cover 3 is placed on the separation cup 1, positioning the stirring rod 7 inside the separation cup 1. Rotating the rotating rod 13 causes the drive plate 11 to rotate. The fan-shaped end of the drive plate 11 slides past the inner side of the arc-shaped plate 10, applying a pushing force to the arc-shaped plate 10. Subsequently, the other end of the drive plate 11 drives the drive rod 12 into the chute 8. Inside the anti-slip pad 9, a pushing force is applied to the slide groove 8. The anti-slip pad 9 can increase the friction with the surface of the drive rod 12, thereby enhancing the pushing force on the slide groove 8. With the cooperation of the drive plate 11, the arc plate 10 and the drive rod 12, the base plate 6 is driven to rotate. When the rotating rod 13 rotates once, it can drive the base plate 6 and the stirring rod 7 to rotate once, thereby stirring the cell suspension to ensure uniform cell distribution and prevent cells from agglomerating or settling during the dilution process, thereby ensuring the uniformity of the cell concentration after dilution and avoiding affecting the accuracy and efficiency of cell separation.
[0024] Please see Figure 1-5 As shown, the separation cup 1 has symmetrically arranged fixing grooves 17 inside. A baffle 18 is rotatably connected to the connecting shaft 16. A sealing gasket 19 is fixedly connected to the outside of the baffle 18. Both the baffle 18 and the sealing gasket 19 are in contact with the inner wall of the separation cup 1. A perforated plate 20 is rotatably connected to the connecting shaft 16. A spring 21 is fixedly connected to one side of both the baffle 18 and the perforated plate 20. A positioning pad 22 is fixedly connected to one end of the spring 21. One side of each of the two sets of positioning pads 22 is fixedly connected to one side of the baffle 18 and the perforated plate 20, respectively. The two sets of positioning pads 22 are movably connected inside the two sets of fixing grooves 17.
[0025] After the single-cell suspension is diluted, the baffle 18 and sealing gasket 19 are rotated around the connecting shaft 16, causing the positioning pad 22 on their surface to move out of a set of fixing grooves 17, allowing the diluted cell suspension to fall onto the porous plate 20, thus enabling the separation of single cells. Subsequently, the porous plate 20 is rotated around the connecting shaft 16, causing the positioning pad 22 and spring 21 at its bottom to move out of another set of fixing grooves 17. The baffle 18 and sealing gasket 19 are rotated in the opposite direction to seal the bottom of the separation cup 1, allowing the separated single cells on the porous plate 20 to be collected. The positioning pads 22 on the baffle 18 and the porous plate 20 are located on the outside of the two sets of fixing grooves 17, respectively. The spring 21 pushes the two sets of positioning pads 22 into the fixing grooves 17, thereby fixing the baffle 18 and the porous plate 20, which facilitates subsequent use.
[0026] The working principle is as follows: Cell suspension is added to separation cup 1, then the top cover 3 is placed on separation cup 1, positioning the stirring rod 7 inside. Rotating the rotating rod 13 causes the drive plate 11 to rotate. The fan-shaped end of the drive plate 11 slides over the inner side of the arc-shaped plate 10, applying a pushing force to the arc-shaped plate 10. Then, the other end of the drive plate 11 drives the drive rod 12 into the anti-slip pad 9 within the chute 8, applying a pushing force to the chute 8. With the cooperation of the drive plate 11, the arc-shaped plate 10, and the drive rod 12, the bottom plate 6 rotates. One rotation of the rotating rod 13 causes one rotation of the bottom plate 6 and the stirring rod 7, thus achieving cell suspension rotation. The suspension is stirred to ensure uniform cell distribution. After the single-cell suspension is diluted, the baffle 18 and sealing gasket 19 are rotated around the connecting shaft 16, causing the positioning pad 22 on their surface to move out of a set of fixing grooves 17, so that the diluted cell suspension falls onto the porous plate 20, allowing the single cells to be separated. The baffle 18 and sealing gasket 19 are rotated in the opposite direction to seal the bottom of the separation cup 1. Then, the porous plate 20 is rotated around the connecting shaft 16, causing the positioning pad 22 and spring 21 at its bottom to move out of another set of fixing grooves 17, allowing the separated single cells on the porous plate 20 to be collected.
Claims
1. A single-cell sterile printing and separation device, characterized in that: include: A separation cup (1) is fixedly connected to a transparent plate (2) on one side. A top cover (3) is movably connected to the top of the separation cup (1). A support groove (4) is provided inside the top cover (3). A limiting groove (5) is provided on the top of the top cover (3). A bottom plate (6) is rotatably connected inside the support groove (4). A stirring rod (7) is fixedly connected to the lower surface of the bottom plate (6). The stirring rod (7) is rotatably connected inside the top cover (3) and extends into the interior of the separation cup (1). A sliding groove (8) is provided on the upper surface of the bottom plate (6). An anti-slip pad (9) is fixedly connected to the inner wall of the sliding groove (8). An arc plate (10) is fixedly connected to the upper surface of the bottom plate (6). A drive plate (11) is drivenly connected to one side of the arc plate (10). A drive rod (12) is fixedly connected to the lower surface of one end of the drive plate (11).
2. The single-cell sterile printing and separation device according to claim 1, characterized in that: The drive rod (12) is connected to the slide groove (8) and the anti-slip pad (9) in a transmission connection. A rotating rod (13) is fixedly connected to the upper surface of the drive plate (11). The rotating rod (13) is rotatably connected to the inside of the upper cover (3) and extends to the top of the upper cover (3).
3. The single-cell sterile printing and separation device according to claim 2, characterized in that: A connecting frame (14) is fixedly connected to the rotating rod (13), and a limiting block (15) is fixedly connected to the bottom of the connecting frame (14). The limiting block (15) is slidably connected inside the limiting groove (5), and a connecting shaft (16) is fixedly connected to one side of the separating cup (1).
4. The single-cell sterile printing and separation device according to claim 3, characterized in that: The separation cup (1) has symmetrically opened fixing grooves (17) inside, and a baffle (18) is rotatably connected to the connecting shaft (16). A sealing gasket (19) is fixedly connected to the outside of the baffle (18).
5. The single-cell sterile printing and separation device according to claim 4, characterized in that: The baffle (18) and the sealing gasket (19) are both in contact with the inner wall of the separation cup (1). A perforated plate (20) is rotatably connected to the connecting shaft (16). A spring (21) is fixedly connected to one side of both the baffle (18) and the perforated plate (20).
6. The single-cell sterile printing and separation device according to claim 5, characterized in that: One end of the spring (21) is fixedly connected to a positioning pad (22). One side of each of the two sets of positioning pads (22) is fixedly connected to one side of the baffle (18) and the perforated plate (20), respectively. The two sets of positioning pads (22) are movably connected inside the two sets of fixing grooves (17).