Simple cell mouth suction tube

Abstract

The utility model relates to a simple cell mouth suction pipe which comprises a mouth suction section at the front end, a connecting section at the middle end and a suction / outlet section at the rear end. The mouth suction section comprises a straight pipe and a filter, one end of the straight pipe is connected with one end of the filter, the connecting section comprises a hose, one end of the hose is connected with the other end of the filter, the suction / outlet section comprises a hard connecting pipe, one end of the hard connecting pipe is connected with the other end of the hose, and the other end of the hard connecting pipe is connected with a rubber plug. The center of the rubber plug is provided with an insertion hole into which a glass needle is inserted. The simple cell mouth suction tube provided by the utility model can effectively solve the problem that saliva flows into and gathers in the mouth suction tube, and improves the efficiency and success rate of experimental operation.

Description

Technical Field

[0001] This invention belongs to the field of in vitro embryo research experimental technology and relates to a simple cell pipette. Background Technology

[0002] In in vitro embryo research of mammals, embryo manipulation is an essential experimental technique, whether it's collecting naked eggs or fertilized eggs, or transferring, washing, and transplanting fertilized eggs. Currently, most operators use self-made straight-through oral pipettes. Typically, researchers must hold one end of the pipette in their mouth and use their mouth to blow or inhale into the pipette to control the transfer or washing of cells at the other end. Usually, researchers must keep the pipette in their mouth throughout a complete cell experiment. During normal breathing, saliva flows into and accumulates in the pipette, leading to unstable and uncontrollable airflow during the experiment. In severe cases, it can clog the pipette, thus affecting the efficiency and success rate of the experiment. Summary of the Invention

[0003] This invention proposes a simple cell pipette that can effectively solve the problem of saliva inflow and accumulation in the pipette, thereby improving the efficiency and success rate of experimental operations.

[0004] The technical solution of this utility model to solve the above problems is:

[0005] This invention proposes a simple cell pipette, which is unique in that:

[0006] It consists of three parts: the mouth-suction section at the front, the connecting section in the middle, and the inhalation / exhalation section at the rear.

[0007] The mouth-suction section includes a straight tube and a filter. One end of the straight tube is connected to one end of the filter. Furthermore, a saliva collection port is provided in the middle of the straight tube, and a saliva collector is connected to the saliva collection port. The saliva collector is a sealed container.

[0008] The connecting section includes a flexible tube, one end of which is connected to the other end of the filter; the inhalation / outhalation section includes a rigid connecting tube, one end of which is connected to the other end of the flexible tube, and the other end is connected to a rubber stopper. The rubber stopper has an insertion hole in the center, into which a glass needle is inserted.

[0009] Furthermore, the straight tube is either a round tube or a triangular tube. When the straight tube is a triangular tube, the saliva collection port is located on one side of the triangular tube. In use, the wall surface connected to the saliva collection port serves as the bottom surface, and saliva flows from the inner walls on both sides to the bottom surface where the saliva collection port is located, ensuring effective collection of saliva.

[0010] Furthermore, the straight pipe has a bend in the middle, and the straight pipes on both sides of the bend are coaxially arranged, with the saliva collection port located at the lowest point of the bend. In use, the bend is oriented downwards, with the saliva collection port at its lowest point. Saliva flows from the higher positions on both sides of the bend to the lowest saliva collection port, ensuring effective collection of saliva.

[0011] Furthermore, the end of the straight pipe furthest from the filter is connected to a flat, round duckbill structure, and the outside of the duckbill structure is provided with a blocking protrusion.

[0012] Furthermore, the thickness of the duckbill structure gradually decreases from the end towards the blocking protrusion.

[0013] Furthermore, the tail end of the duckbill structure is inserted into the straight tube. The duckbill structure is made of silicone.

[0014] Furthermore, the filter is used to filter water vapor in the airflow. It includes an upper cover and a lower cover, which are connected by threads. The upper and lower covers form a cavity inside, and the cavity is provided with a breathable filter material. The breathable filter material includes a spunbond nonwoven fabric layer and a meltblown nonwoven fabric layer. The spunbond nonwoven fabric is close to the upper cover and has a waterproof function. The meltblown nonwoven fabric is made of polypropylene material with high melt index fiber and is an ultra-fine electrostatic fiber cloth that can effectively use electrostatic adsorption of viral dust and droplets.

[0015] Furthermore, the filter has connecting pipes on its upper and lower covers. The connecting pipe of the upper cover is connected to a straight pipe, and the connecting pipe of the lower cover is connected to a flexible hose.

[0016] Furthermore, the saliva collector is connected to the saliva collection port in the middle of the straight pipe by a thread, making it easy to replace.

[0017] Furthermore, one end of the straight pipe connecting filter is provided with a connecting end, the outer diameter of which is smaller than the outer diameter of the straight pipe.

[0018] Advantages of this utility model:

[0019] The simple cell pipette provided by this invention has a simple overall structure and can effectively solve the problem of saliva inflow and accumulation in the pipette, thereby improving the efficiency and success rate of experimental operations. Attached Figure Description

[0020] Figure 1 This is a structural diagram of one embodiment of a simple cell mouth pipette;

[0021] Figure 2 This is a structural diagram of the duckbill structure;

[0022] Figure 3 This is a structural diagram of another embodiment of a simple cellular mouth pipette.

[0023] In the diagram, 1. Straight tube; 2. Filter; 3. Hose; 4. Rigid connecting tube; 5. Rubber stopper; 6. Glass needle; 7. Duckbill structure; 8. Blocking protrusion; 9. Top cover; 10. Bottom cover; 11. Breathable filter material; 12. Saliva collector. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this utility model clearer, the technical solutions of the embodiments of this utility model will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only a part of the embodiments of this utility model, not all of them. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model. Therefore, the following detailed description of the embodiments of this utility model provided in the accompanying drawings is not intended to limit the scope of the claimed utility model, but merely to represent selected embodiments of this utility model.

[0025] See Figure 1 and Figure 3 A simple cellular pipette, consisting of three parts: a front mouthpiece, a middle connecting section, and a rear inhalation / exhalation section.

[0026] The mouth-suction section includes a straight tube 1 and a filter 2. One end of the straight tube 1 is connected to one end of the filter 2. A saliva collection port is provided in the middle of the straight tube 1, and a saliva collector 12 is connected to the saliva collection port. The saliva collector 12 is a sealed container. The connecting section includes a flexible tube 3, which is a rubber tube. One end of the flexible tube 3 is connected to the other end of the filter 2. The suction / exhaust section includes a rigid connecting tube 4. One end of the rigid connecting tube 4 is connected to the other end of the flexible tube 3, and the other end is connected to a rubber stopper 5. The rubber stopper 5 has an insertion hole in its center, into which a glass needle 6 is inserted.

[0027] In in vitro embryonic research on mammals, researchers need to perform routine experimental procedures on oocytes or embryonic cells (hereinafter referred to as "cells"), such as transfer between different culture media, post-IVF washing, and in vivo transplantation. During specific cell manipulation experiments, researchers must hold one end of the straight tube 1 in their mouth and blow / inhale into the pipette. The airflow passes sequentially through the straight tube 1, filter 2, flexible tube 3, rigid connecting tube 4, and glass needle 6, thereby controlling the entry, exit, transfer, washing, or transplantation of cells at the other end of the pipette. Typically, researchers must keep the pipette in their mouth throughout a complete cell experiment. During normal breathing, saliva will flow in. A saliva collector 12 is connected to the saliva collection port in the middle of the straight tube 1. When in use, the saliva collector 12 faces downwards, and the saliva enters the saliva collector 12. Water vapor in the airflow enters the filter 2, which filters the water vapor. This prevents saliva and water vapor from clogging the pipette, ensuring stable airflow during the experiment and thus improving the efficiency and success rate of the experiment.

[0028] In a preferred embodiment of the present invention, the saliva collector 12 includes a soft capsule. When the saliva collector 12 needs to be cleaned, the saliva collector 12 is removed from the saliva collection port, and the capsule is squeezed with fingers to quickly drain the saliva from the capsule.

[0029] In a preferred embodiment of this utility model, the straight pipe 1 is a circular pipe, or the straight pipe 1 is a triangular pipe (with a triangular cross-section). When the straight pipe 1 is a triangular pipe, the saliva collection port is located on one side of the triangular pipe. In use, the wall surface connected to the saliva collection port serves as the bottom surface, so that saliva flows from the inner walls on both sides to the bottom surface where the saliva collection port is located, ensuring effective collection of saliva.

[0030] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 3 The straight pipe 1 has a bend in the middle, and the straight pipes on both sides of the bend are coaxially arranged. The saliva collection port is located at the lowest point of the bend. In use, the bend is oriented downwards, and the saliva collection port is at the lowest point. Saliva flows from the higher positions on both sides of the bend to the lowest saliva collection port, ensuring effective collection of saliva.

[0031] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 1 and Figure 2 The straight tube 1, at the end furthest from the filter 2, is connected to a flat, round duckbill structure 7. A blocking protrusion 8 is provided on the outer side of the duckbill structure 7. The duckbill structure 7 is made of silicone, making it easy for the operator to place in their mouth. The flat, round shape of the duckbill structure 7 prevents it from rotating, facilitating operation. The blocking protrusion 8 serves to limit its movement, preventing the duckbill structure 7 from extending too far into the operator's mouth.

[0032] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 2 The thickness of the duckbill structure 7 gradually decreases from the end towards the blocking protrusion 8, so the operator does not have to worry about the duckbill structure 7 slipping out of the mouth, allowing the operator to use both hands to perform other operations.

[0033] In a preferred embodiment of this utility model, the tail of the duckbill structure 7 is inserted into the straight tube 1 and is detachably connected, which facilitates the cleaning and replacement of the duckbill structure 7.

[0034] In a preferred embodiment of this utility model, the filter 2 is used to filter water vapor in the airflow. It includes an upper cover 9 and a lower cover 10, which are connected by threads. A cavity is formed inside the upper cover 9 and the lower cover 10, and a breathable filter material 11 is provided inside the cavity. The breathable filter material 11 includes a spunbond nonwoven fabric layer and a meltblown nonwoven fabric layer. The spunbond nonwoven fabric is located near the upper cover and has waterproof properties, while the meltblown nonwoven fabric is made of polypropylene material with a high melt index fiber. It is an ultra-fine electrostatic fiber cloth that can effectively utilize electrostatic adsorption of viral dust and droplets.

[0035] As a preferred embodiment of this utility model, see [link to relevant documentation]. Figure 1 The filter 2 has connecting pipes on its upper cover 9 and lower cover 10. The connecting pipe of the upper cover 9 is inserted into the end of the straight pipe 1, and the connecting pipe of the lower cover 10 is sleeved with the hose 3.

[0036] In a preferred embodiment of this utility model, the saliva collector 12 is detachably connected to the saliva collection port in the middle of the straight pipe 1 by means of threads, which facilitates cleaning the saliva inside the saliva collector 12.

[0037] In a preferred embodiment of this utility model, the straight pipe 1 is provided with a connecting end at one end connected to the filter 2. The outer diameter of the connecting end is smaller than the outer diameter of the straight pipe 1, making it easy for the flexible hose 3 to be fitted onto the connecting end. The flexible hose 3 is approximately 15-20cm long, 7mm in diameter, and 5mm in inner diameter, which ensures stable airflow.

[0038] The straight tube 1 and the saliva collector 12 are made of PPSU material, which can withstand high-temperature sterilization and can be reused. The rubber stopper 5 has a small hole in the middle, which can stably support the glass needle and ensure a tight seal when the glass needle is inserted. The glass capillary is a conventional consumable product, and the glass needle can be drawn under an alcohol lamp. The inner diameter of the tip is large enough to draw in mouse or rat embryonic cells.

[0039] The above description is merely an embodiment of this utility model and is not intended to limit the scope of protection of this utility model. Any equivalent structural or procedural transformations made based on the description and drawings of this utility model, or direct or indirect applications in other related system fields, are similarly included within the scope of protection of this utility model.

Claims

1. A simple cell-based pipette, characterized in that: It consists of three parts: the mouth-suction section at the front, the connecting section in the middle, and the inhalation / exhalation section at the rear. The mouth suction section includes a straight tube (1) and a filter (2). One end of the straight tube (1) is connected to one end of the filter (2). A saliva collection port is provided in the middle of the straight tube (1), and a saliva collector (12) is connected to the saliva collection port. The saliva collector (12) is a sealed container. The connecting section includes a hose (3), one end of which is connected to the other end of the filter (2); The inhalation / exhalation section includes a rigid connecting tube (4), one end of which is connected to the other end of the flexible tube (3), and the other end is connected to a rubber stopper (5). The rubber stopper (5) has an insertion hole in the center, and a glass needle (6) is inserted into the insertion hole.

2. The simple cell pipette according to claim 1, characterized in that: The straight pipe (1) is either a round pipe or a triangular pipe. When the straight pipe (1) is a triangular pipe, the saliva collection port is located on one side of the triangular pipe.

3. The simple cell pipette according to claim 1, characterized in that: The straight pipe (1) has a bend in the middle, and the straight pipes on both sides of the bend are coaxially arranged, with the saliva collection port located at the lowest point of the bend.

4. A simple cell pipette according to any one of claims 1-3, characterized in that: The straight tube (1) is connected to a flat, round duckbill structure (7) at the end away from the filter (2), and a blocking protrusion (8) is provided on the outside of the duckbill structure (7).

5. A simple cell pipette according to claim 4, characterized in that: The thickness of the duckbill structure (7) gradually decreases from the end toward the blocking protrusion (8).

6. A simple cell pipette according to claim 5, characterized in that: The tail of the duckbill structure (7) is inserted into the straight tube (1); the duckbill structure (7) is made of silicone.

7. A simple cell pipette according to claim 6, characterized in that: The filter (2) is used to filter water vapor in the airflow. It includes an upper cover (9) and a lower cover (10), which are connected by threads. The upper cover (9) and the lower cover (10) form a cavity inside the upper cover (9) and the lower cover (10). The cavity is provided with a breathable filter material (11), which includes a spunbond nonwoven fabric layer and a meltblown nonwoven fabric layer.

8. A simple cell pipette according to claim 7, characterized in that: The filter (2) has connecting pipes on its upper cover (9) and lower cover (10). The connecting pipe of the upper cover (9) is connected to the straight pipe (1), and the connecting pipe of the lower cover (10) is connected to the flexible hose (3).

9. A simple cell pipette according to claim 8, characterized in that: The saliva collector (12) is connected to the saliva collection port in the middle of the straight pipe (1) by a thread.

10. A simple cell pipette according to claim 9, characterized in that: The straight pipe (1) is connected to the filter (2) at one end, and the outer diameter of the connecting end is smaller than the outer diameter of the straight pipe (1).

Images