Combined chromatographic column

Abstract

The utility model relates to a combined chromatographic column, which comprises a plurality of separation units which are sequentially and detachably connected from top to bottom, each separation unit comprises a pipe body, an upper membrane for liquid to flow through is arranged at the upper part of the pipe body, and a lower membrane for liquid to flow through is arranged at the lower part of the pipe body. An area between the upper membrane and the lower membrane is a filler area which is used for containing filler and can prevent the filler from leaking out, a splitter plate is arranged above the upper membrane in each pipe body, a plurality of vertically-through liquid passing holes are formed in the splitter plate, a liquid passing channel which enables the liquid passing holes to be communicated is formed in the upper surface of the splitter plate, and the liquid passing channels are communicated with the upper membrane and the lower membrane. The separation effect is good.

Description

TECHNICAL FIELD

[0001] The utility model relates to the technical field of chromatographic column, specifically relates to a combined chromatographic column. BACKGROUND

[0002] At present, chromatography is all from one end of the chromatographic column sample, using appropriate solvent elution, the different substances in the mixture from one end of the chromatographic column elution to the other end, and then collected in turn. This method elution time is longer, solvent consumption is large.

[0003] For the above problems, the Chinese patent application for invention with patent No. CN201410006879.0 (publication No. CN103752040A) discloses "modular chromatographic column and its use", the chromatographic column is assembled by a top cover, a plurality of separation modules, a bottom cover and a plurality of sealing pressure rings, the separation module is a circular tube, filled with filler, the upper and lower sides of the filler are placed with sintered glass plate or membrane or net, each separation module and the uppermost and lowermost two separation modules and the top end and bottom end cover are fixedly connected in a detachable manner by thread or buckle. Using the modular chromatographic column, after sample injection, the sample is eluted with washing liquid, once the mobile phase reaches the sample outlet, or the sample is separated by external observation or detection during elution, the elution can be stopped, then the chromatographic column is disassembled from each connection part, and the separation modules are individually washed with solvent, and the individual components can be obtained after drying. The scheme is simple to operate, can greatly save the amount of solvent or washing liquid, elution is faster, and expensive fraction collection equipment is not needed.

[0004] But the mixture to be separated and the eluent of the patent are all from the through hole of the top cover, as can be seen from the figure, the through hole is located at the central part of the top cover and the flow area is smaller than the area of the top cover, so that the mixture and the solvent directly flow down along the through hole, part of the separation filler (i.e. filler) does not pass through the mixture and the solvent, and the separation effect is poor, especially when the separation filler is unevenly filled, the phenomenon will be more obvious. In addition, for this combined chromatographic column, because the fillers of each separation module are filled separately, it is more likely that the fillers of different separation modules have different filling densities, resulting in uneven flow of the mobile phase. UTILITY MODEL CONTENTS

[0005] The utility model solves the technical problem in the prior art, and provides a combined chromatographic column capable of making liquid flow uniformly.

[0006] The utility model discloses a technical scheme that solves the above technical problems: a combined chromatographic column, comprising a plurality of self top-to-bottom detachable connecting separation units, each separation unit comprises a pipe body, the upper portion of the pipe body is provided with an upper diaphragm for liquid flow, the lower portion of the pipe body is provided with a lower diaphragm for liquid flow, the area between the upper diaphragm and the lower diaphragm is a filler area for accommodating filler and preventing filler leakage, characterized in that each pipe body is provided with a flow distribution plate above the upper diaphragm, the flow distribution plate is provided with a plurality of vertical through liquid flow holes, and the upper surface of the flow distribution plate is provided with a liquid flow channel for connecting the liquid flow holes.

[0007] In order to make the liquid flow uniformly downward, the liquid flow hole at the central position of the flow distribution plate is used as a center hole, and a plurality of liquid flow hole groups are uniformly distributed along the radial direction around the center hole, each liquid flow hole group comprises a plurality of liquid flow holes uniformly distributed along the circumference of the center hole.

[0008] The liquid flow channel comprises a plurality of first channels and a plurality of second channels, each first channel is annular and extends along the circumference of the center hole, and a plurality of second channels are distributed radially around the center hole, the liquid flow holes in the same group are connected by the first channels, and the liquid flow holes in different groups are connected by the second channels. The transmission of liquid through the liquid flow channel makes liquid flow at each liquid flow hole, and the liquid flow holes are uniformly arranged on the flow distribution plate and flow downward uniformly.

[0009] In order to realize the disassembly of adjacent two separation units, the pipe body is provided with an upper joint above the flow distribution plate and a lower joint below the lower diaphragm, the upper joint of one separation unit and the lower joint of another separation unit are detachably connected, the upper joint is provided with a first liquid flow channel for liquid flow, the lower joint is provided with a second liquid flow channel for liquid flow, and the first liquid flow channel and the second liquid flow channel are connected in the connected state of the upper joint and the lower joint.

[0010] The upper joint and the lower joint can be connected by buckling or magnetic attraction, in order to make the connection between the upper joint and the lower joint firm and prevent them from loosening under pressure, the upper joint comprises a plate body and a screw rod vertically installed on the plate body, the lower joint is a nut, the screw rod can be threadedly connected with the nut, the first liquid flow channel vertically penetrates the screw rod and the plate body, and the second liquid flow channel corresponds to the hollow area of the nut in the vertical direction; or the lower joint comprises a plate body and a screw rod vertically installed on the plate body, the upper joint is a nut, the screw rod can be threadedly connected with the nut, the first liquid flow channel vertically penetrates the nut, and the second liquid flow channel vertically penetrates the screw rod and the plate body. The threaded connection of the upper joint and the lower joint not only makes them easy to disassemble, but also makes them difficult to loosen in the connected state.

[0011] In order to prevent liquid from leaking out between the upper joint and the pipe body, the upper joint is in sealing contact with the pipe body, and in order to prevent liquid from leaking out between the lower joint and the pipe body, the lower joint is in sealing contact with the pipe body. The upper joint and the lower joint not only play a role in connecting two adjacent separation units, but also play a sealing role.

[0012] In order to prevent the upper diaphragm from being insufficient in strength to press the filler, an upper supporting sheet with a strength greater than that of the upper diaphragm is arranged between the flow distribution plate and the upper diaphragm.

[0013] In order to prevent the lower diaphragm from being insufficient in strength to support the filler, a lower supporting sheet with a strength greater than that of the lower diaphragm is arranged between the lower joint and the lower diaphragm.

[0014] Preferably, the central part of the lower supporting sheet is concave downward, so that the separated sample can be quickly formed into a water droplet type and gathered in the middle, and the concave structure in the middle makes the middle filler thicker, avoids cracking of the middle filler due to continuous driving of pressure, and makes the structure more flat.

[0015] In the above scheme, the combined chromatographic column further comprises a feed pipe and a discharge pipe;

[0016] When there is only one separation unit, the feed pipe is connected to and in fluid communication with the upper joint of the separation unit, and the discharge pipe is connected to and in fluid communication with the lower joint of the separation unit;

[0017] When there are at least two separation units, the feed pipe is connected to and in fluid communication with the upper joint of the uppermost separation unit, and the discharge pipe is connected to and in fluid communication with the lower joint of the lowermost separation unit.

[0018] Compared with the prior art, the advantages of the utility model are: the utility model discloses a flow distribution plate is arranged in the pipe body of each separation unit, so that liquid can be uniformly distributed in the filler of each separation unit, and the separation effect is improved. BRIEF DESCRIPTION OF DRAWINGS

[0019] Figure 1 It is a sectional view of one separation unit of the utility model embodiment;

[0020] Figure 2 It is a structure schematic view of the separation unit of the utility model embodiment; Figure 1

[0021] Figure 3 It is​Figure 2 A schematic diagram of the structure from another direction;

[0022] Figure 4 for Figure 3 A sectional view;

[0023] Figure 5 for Figure 3 A schematic diagram of the decomposition process;

[0024] Figure 6 for Figure 5 A schematic diagram of the structure of the flow divider. Detailed Implementation

[0025] The present invention will be further described in detail below with reference to the accompanying drawings and embodiments.

[0026] like Figures 1 to 6 As shown, the combined chromatographic column of this preferred embodiment includes multiple separation units that are detachably connected in sequence from top to bottom.

[0027] Each separation unit includes a tube (not shown in the figure), which can be a vertically penetrating glass tube or plastic tube. The tube contains, from top to bottom, an upper connector 1, a diverter plate 7, an upper support plate 2, an upper diaphragm 3, a lower diaphragm 4, a lower support plate 5, and a lower connector 6.

[0028] The upper part of the tube is provided with an upper diaphragm 3 for liquid to flow through, and the lower part of the tube is provided with a lower diaphragm 4 for liquid to flow through. The area between the upper diaphragm 3 and the lower diaphragm 4 is a packing area 0 for accommodating the packing material and preventing leakage of the packing material 01. The packing material can be silica gel or graphene, etc.

[0029] Each tube has a flow divider plate 7 above the upper diaphragm 3. The flow divider plate 7 has multiple vertically penetrating liquid passage holes 71, and the upper surface of the flow divider plate 7 has a liquid passage channel that connects the liquid passage holes 71.

[0030] In this embodiment, the liquid passage 71 located in the center of the flow divider 7 is used as the central hole. Figure 6 (The hole marked a) has multiple groups of liquid passage holes evenly distributed radially around the central hole. Each group of liquid passage holes includes multiple liquid passage holes 71 evenly distributed circumferentially around the central hole.

[0031] The liquid-passing channel includes multiple first channels 72 and multiple second channels 73. Each first channel 72 is annular and extends circumferentially along the central hole. The multiple second channels 73 are radially distributed around the central hole. Liquid-passing holes 71 in the same group are connected through the first channels 72, and liquid-passing holes 71 in different groups are connected through the second channels 73. This liquid-passing channel ensures that liquid flows at each liquid-passing hole 71, and the liquid-passing holes 71 are evenly arranged on the distribution plate 7, resulting in uniform downward flow.

[0032] The embodiment realizes the disassembly of the two adjacent separation units in the following manner:

[0033] The pipe body is provided with an upper joint 1 above the flow distribution plate 7 and a lower joint 6 below the lower diaphragm 4, the upper joint 1 of one of the two adjacent separation units is detachably connected with the lower joint 6 of the other separation unit, the upper joint 1 is provided with a first liquid flow channel 13 for liquid flow, and the lower joint 6 is provided with a second liquid flow channel 61 for liquid flow.

[0034] In the embodiment, the upper joint 1 comprises a plate body 11 and a screw rod 12 vertically installed on the plate body 11, the plate body 11 and the screw rod 12 are integrally formed, the first liquid flow channel 13 vertically penetrates the screw rod 12 and the plate body 11, the lower joint 6 is a screw cap, the second liquid flow channel 61 corresponds to a hollow area 62 of the screw cap in the vertical direction, the screw rod 12 is threadedly connected with the screw cap, and in the state that the screw rod 12 is connected with the screw cap, the first liquid flow channel 13 and the second liquid flow channel 61 are in communication. The threaded connection of the upper joint 1 and the lower joint 6 not only makes the disassembly convenient, but also makes the two not easy to be loosened in the connected state.

[0035] Of course, the lower joint 6 can also comprise a plate body 11 and a screw rod 12 vertically installed on the plate body 11, the upper joint 1 is a screw cap, the screw rod 12 is threadedly connected with the screw cap, the first liquid flow channel 13 vertically penetrates the screw cap, and the second liquid flow channel 61 vertically penetrates the screw rod 12 and the plate body 11.

[0036] In order to prevent liquid from leaking out between the upper joint 1 and the pipe body, the upper joint 1 is in sealing contact with the pipe body, and in order to prevent liquid from leaking out between the lower joint 6 and the pipe body, the lower joint 6 is in sealing contact with the pipe body. The upper joint 1 and the lower joint 6 not only play a role in connecting the two adjacent separation units, but also play a sealing role. The upper joint 1 and the lower joint 6 can directly use flexible materials as sealing members, or sealing rings can be arranged between the upper joint 1 and the pipe body and between the lower joint 6 and the pipe body.

[0037] In order to prevent the strength of the upper diaphragm 3 from being insufficient to press the filler, an upper supporting sheet 2 for fluid flow is arranged between the flow distribution plate 7 and the upper diaphragm 3, the strength of the upper supporting sheet 2 is greater than that of the upper diaphragm 3, and the upper supporting sheet 2 and the upper diaphragm 3 are both internally provided with a mesh structure. In the embodiment, the pore size of the upper supporting sheet 2 is greater than that of the upper diaphragm 3, and of course, the pore sizes of the two can not be limited.

[0038] In order to prevent the lower membrane 4 from being insufficient in strength to support the filler, a lower supporting sheet 5 through which fluid can flow is arranged between the lower joint 6 and the lower membrane 4, the lower supporting sheet 5 is greater in strength than the lower membrane 4, and the lower supporting sheet 5 and the lower membrane 4 are both internally provided with a mesh structure. In the embodiment, the pore size of the lower supporting sheet 5 is greater than that of the lower membrane 4. Of course, the pore sizes of the two can not be limited.

[0039] The central part of the lower supporting sheet 5 is concave, so that the separated sample can quickly form a water droplet type and gather in the middle, and the concave structure in the middle makes the middle filler thicker, avoids cracking due to the continuous driving of the pressure on the middle filler, and makes the structure more flat.

[0040] The combined chromatographic column of the embodiment further comprises a feed pipe 8 and a discharge pipe 9. The feed pipe 8 can be used for the eluent and the mixture to be separated to enter the pipe body, and the discharge pipe 9 can be used for the eluent and the compounds eluted by the eluent to flow out. When there is only one separation unit, the feed pipe 8 is connected to and in fluid communication with the upper joint 1 of the separation unit, and the discharge pipe 9 is connected to and in fluid communication with the lower joint 6 of the separation unit.

[0041] When there are at least two separation units, the feed pipe 8 is connected to and in fluid communication with the upper joint 1 of the uppermost separation unit, and the discharge pipe 9 is connected to and in fluid communication with the lower joint 6 of the lowermost separation unit.

[0042] Specifically, in the embodiment, the feed pipe 8 is threadedly connected to the screw rod 12 of the upper joint 1, and the feed pipe 8 is connected to the first liquid flow channel 13 to realize fluid communication; the discharge pipe 9 is threadedly connected to the lower joint 6, and the discharge pipe 9 is connected to the second liquid flow channel 61 to realize fluid communication.

[0043] When the combined chromatographic column of the embodiment is used, a large number of separation units can be used when a large amount of mixture needs to be separated, and a small number of separation units can be used when a small amount of mixture needs to be separated.

[0044] The following scenarios can also be applicable: when the eluent reaches the sample outlet, or when the mixture is observed or detected to have been separated during the elution process, the elution can be stopped, and then the separation units can be disassembled, and the separation units can be separately washed with the eluent, and after drying, the corresponding components can be obtained.

[0045] In the description of the utility model, need understanding is, the term '' center '' '' longitudinal '' '' transverse '' '' length '' '' width '' '' thickness '' '' upper '' '' lower '' '' front '' '' rear '' '' left '' '' right '' '' vertical '' '' horizontal '' '' top '' '' bottom '' '' inner '' '' outer '' '' clockwise '' '' counterclockwise '' '' axial '' '' radial '' '' circumferential '' etc. Indicated orientation or positional relationship is based on the orientation or positional relationship shown in the drawing, only for the convenience of describing the utility model and simplifying the description, and is not indicated or implied that the device or element indicated must have a particular orientation, a particular orientation structure and operation, since the disclosed embodiment of the utility model can be arranged in different directions, so these orientation terms are only as an illustration and should not be regarded as a limitation, for example '' upper '' '' lower '' not necessarily be limited to the direction opposite or consistent with the direction of gravity.

Claims

1. A combined chromatographic column comprising a plurality of separation units which are detachably connected one after another from top to bottom, each of said separation units comprising a tube body, an upper membrane (3) through which a liquid can flow being provided at an upper portion of said tube body, a lower membrane (4) through which a liquid can flow being provided at a lower portion of said tube body, a region between the upper membrane (3) and the lower membrane (4) being a packing zone (0) for accommodating a packing material and for preventing the packing material from leaking out, characterized in that Each of the pipe bodies is provided with a flow distribution plate (7) above the upper diaphragm (3), the flow distribution plate (7) is provided with a plurality of vertical through liquid holes (71), and the upper surface of the flow distribution plate (7) is provided with a liquid passing channel for connecting the liquid holes (71).

2. The modular chromatography column of claim 1, wherein: The liquid passing holes (71) in the central part of the flow distribution plate (7) are taken as center holes, and a plurality of liquid passing hole groups are distributed in the radial direction in sequence and at equal intervals around the center holes, each liquid passing hole group includes a plurality of liquid passing holes (71) distributed at equal intervals around the center hole; The liquid passing channel includes a plurality of first channels (72) and a plurality of second channels (73), each of the first channels (72) is annular and extends along the circumference of the center hole, and the second channels (73) are distributed radially around the center hole, the liquid passing holes (71) in the same group are connected through the first channels (72), and the liquid passing holes (71) in different groups are connected through the second channels (73).

3. The modular chromatography column of claim 1, wherein: The pipe body is provided with an upper joint (1) above the flow distribution plate (7), and is provided with a lower joint (6) below the lower diaphragm (4), the upper joint (1) of one of the adjacent two separation units is detachably connected with the lower joint (6) of the other separation unit, the upper joint (1) is provided with a first liquid passing flow channel (13) for liquid flow, the lower joint (6) is provided with a second liquid passing flow channel (61) for liquid flow, and the first liquid passing flow channel (13) and the second liquid passing flow channel (61) are connected in communication when the upper joint (1) and the lower joint (6) are connected.

4. The modular chromatography column of claim 3, wherein: The upper joint (1) includes a plate body (11) and a screw rod (12) vertically installed on the plate body (11), the lower joint (6) is a screw cap, the screw rod (12) can be threadedly connected with the screw cap, the first liquid passing flow channel (13) vertically penetrates the screw rod (12) and the plate body (11), and the second liquid passing flow channel (61) corresponds to a hollow area (62) of the screw cap in the vertical direction; or, the lower joint (6) includes a plate body (11) and a screw rod (12) vertically installed on the plate body (11), the upper joint (1) is a screw cap, the screw rod (12) can be threadedly connected with the screw cap, the first liquid passing flow channel (13) vertically penetrates the screw cap, and the second liquid passing flow channel (61) vertically penetrates the screw rod (12) and the plate body (11).

5. The modular chromatography column of claim 3, wherein: The upper joint (1) is in sealed contact with the pipe body, and the lower joint (6) is in sealed contact with the pipe body.

6. The modular chromatography column of claim 1, wherein: The flow distribution plate (7) and the upper diaphragm (3) are provided with an upper support plate (2) having a strength greater than that of the upper diaphragm (3).

7. The modular chromatography column of claim 3, wherein: The lower joint (6) and the lower diaphragm (4) are provided with a lower support plate (5) having a strength greater than that of the lower diaphragm (4).

8. The modular chromatography column of claim 7, wherein: The central part of the lower support plate (5) is concave downward.

9. The modular chromatography column of any one of claims 1-8, wherein: The combined chromatographic column further includes a feed pipe (8) and a discharge pipe (9); When there is only one separation unit, the feed pipe (8) is connected to and in fluid communication with the upper joint (1) of the separation unit, and the discharge pipe (9) is connected to and in fluid communication with the lower joint (6) of the separation unit; When there are at least two separation units, the feed pipe (8) is connected to and in fluid communication with the upper connection (1) of the uppermost separation unit, and the discharge pipe (9) is connected to and in fluid communication with the lower connection (6) of the lowermost separation unit.

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