Automatic culture medium split charging device

Abstract

The utility model belongs to the technical field of culture medium split charging, and particularly relates to an automatic culture medium split charging device which comprises an operation table, a storage barrel, a stirrer, a split charging assembly and a controller, the stirrer is used for stirring the liquid culture medium in the storage barrel; the sub-packaging assembly comprises a tray, a plurality of rows of placing grooves are formed in the tray, and a plurality of tissue culture bottles can be placed in each row of placing grooves; a support is arranged above the tray, a plurality of sub-packaging pipes are arranged on the support, the liquid outlet ends of the sub-packaging pipes are vertically downward and right face bottle openings of the tissue culture bottles, the liquid inlet ends of the sub-packaging pipes are communicated with the interior of the storage barrel, each sub-packaging pipe is provided with a water pump, and the water pumps are used for pumping liquid culture media in the storage barrel and conveying the liquid culture media into the tissue culture bottles through the sub-packaging pipes; the water pump is electrically connected with the controller; the controller is used for controlling the water pump to start or stop; the technical problems that the culture medium split charging efficiency is low, split charging operation is inconvenient, and the requirement for operators is high are solved.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of culture medium subpackaging, and particularly relates to an automatic culture medium subpackaging device. BACKGROUND

[0002] The traditional subpackaging method of tissue culture solid medium is to proportion solid medium (MS powder 14.4g, sucrose 30g, agar 9g, and distilled water 1L), fill the medium into a large-volume triangular bottle, sterilize the medium at high temperature, sterilize the tissue culture bottle at high temperature, pour the sterilized liquid medium (about 50 DEG C, liquid without cooling) into the sterilized tissue culture bottle in a sterile operation table, and subpackaging.

[0003] The subpackaging method has the following disadvantages: 1. The subpackaging amount of the medium is large, the traditional subpackaging method is to subpackaging the liquid medium from the triangular bottle into the tissue culture bottle one by one in a sterile environment, and the subpackaging efficiency is low, and the working strength of the operator is large; 2. In order to ensure the flowability of the medium, the subpackaging temperature needs to be kept at about 50 DEG C, and the operator needs to take heat insulation measures when subpackaging to avoid being scalded, and the operation is inconvenient; 3. The medium is easily contaminated during the subpackaging process, and the operator needs to operate for a long time, and the quality requirement of the operator is high. UTILITY MODEL CONTENT

[0004] The utility model aims at providing an automatic culture medium subpackaging device to solve the technical problems of low subpackaging efficiency, inconvenient subpackaging operation, and high requirement for the operator of the existing subpackaging method.

[0005] In order to solve the above technical problems, the utility model provides the following technical scheme:

[0006] An automatic culture medium subpackaging device comprises an operation table, a storage barrel, a stirrer, a subpackaging assembly and a controller arranged on the operation table; the storage barrel is used for preparing the medium, and the stirrer is used for stirring the liquid medium in the storage barrel; the subpackaging assembly comprises a tray slidably arranged on the operation table, a plurality of rows of placing grooves are arranged on the tray, and each row of placing grooves can place a plurality of tissue culture bottles; a support is arranged above the tray, a plurality of subpackaging pipes are arranged on the support, one end of each subpackaging pipe is a liquid outlet end, the liquid outlet end is vertically downward and opposite to the bottle opening of the tissue culture bottle, the other end of each subpackaging pipe is a liquid inlet end, and the liquid inlet end is in communication with the inside of the storage barrel; a water pump is arranged on each subpackaging pipe, and the water pump is used for pumping the liquid medium in the storage barrel and conveying the liquid medium into the tissue culture bottle through the subpackaging pipe; the water pump is electrically connected with the controller, and the controller is used for controlling the start or stop of the water pump.

[0007] Furthermore, the dispensing tube is also equipped with a solenoid valve and a flow meter. Both the solenoid valve and the flow meter are electrically connected to the controller. The flow meter is used to detect the liquid flow rate of the dispensing tube and send a signal to the controller. The controller is used to receive the signal from the flow meter and control the opening and closing of the solenoid valve.

[0008] Furthermore, the operating table is equipped with a slide rail, a tray is mounted on the slide rail, and an electric push rod is connected to the tray. The electric push rod can drive the tray to move on the slide rail. The electric push rod is electrically connected to a controller, which is used to control the start or stop of the electric push rod.

[0009] Furthermore, the inner side of the bracket is provided with a sensing element electrically connected to the controller. The sensing element is used to detect whether the tissue culture bottle on the tray has moved to directly below the water outlet of the dispensing tube and send a signal to the controller. The controller is used to receive the signal from the sensing element and control the start or stop of the electric push rod and the water pump according to the signal, as well as control the opening and closing of the solenoid valve.

[0010] Furthermore, the stirrer includes a base, a fixed rod on the base, a slidable support rod on the fixed rod, a motor mounted on the support rod, a stirring shaft connected to the motor, a plurality of blades on the stirring shaft, and the motor being able to drive the stirring shaft to rotate; the motor is electrically connected to a controller, and the controller is able to control the starting and stopping of the motor.

[0011] Furthermore, the storage tank includes a detachably connected tank body and a lid, with a through hole on the lid, through which the stirring shaft can pass and be placed inside the tank.

[0012] Furthermore, the operating platform is equipped with a control panel, which is electrically connected to the controller. The control panel can send commands to the controller to start or stop the motor, electric push rod, and water pump, and can also set the dispensing amount of the culture flask.

[0013] Compared with the prior art, the present invention has the following beneficial technical effects:

[0014] 1. This utility model, by setting up a controller, water pump, solenoid valve and flow meter, can accurately control the liquid output of the dispensing tube, thereby ensuring that the volume of liquid culture medium injected into each tissue culture bottle is equal, realizing the automated quantitative dispensing of culture medium; and by setting multiple tissue culture bottles and dispensing tubes on the tray, it can realize the batch dispensing of culture medium, and during the dispensing process, the operator does not need to hold the high-temperature triangular bottle, avoiding hand burns. The dispensing operation is simple and fast, effectively speeding up the dispensing efficiency and reducing the labor intensity of the operator.

[0015] 2. Traditional dispensing methods involve first sterilizing the culture medium at high temperature and then dispensing it on a sterile operating table. However, when using the dispensing device of this utility model, the culture medium is first dispensed and then sterilized. This greatly reduces the risk of contamination of the culture medium, and the dispensing operation does not require professional personnel, thus lowering the requirements for operators.

[0016] 3. Since agar is difficult to dissolve in water at room temperature, this invention uses a stirrer to continuously stir the liquid culture medium in the storage tank, which can fully ensure the uniform distribution of agar powder in the solution and ensure that the culture medium components in each tissue culture bottle are equal. Attached Figure Description

[0017] Figure 1 This is a schematic diagram of the structure of this utility model;

[0018] Figure 2 This is a partial structural schematic diagram of the present invention.

[0019] In the diagram: 1. Control panel, 11. Slide rail, 2. Storage tank, 21. Tank body, 22. Lid, 3. Stirrer, 31. Base, 32. Fixing rod, 33. Support rod, 34. Motor, 35. Stirring shaft, 36. Blade, 4. Dispensing assembly, 41. Tray, 42. Electric push rod, 43. Tissue culture bottle, 44. Support, 45. Dispensing tube, 46. Water pump, 47. Sensor element, 5. Control panel. Detailed Implementation

[0020] The following detailed description illustrates the specific implementation method:

[0021] Example

[0022] like Figure 1 As shown, an automated culture medium dispensing device includes an operating table 1 and a storage tank 2, a stirrer 3, a dispensing component 4, and a controller disposed on the operating table 1.

[0023] like Figures 1-2As shown, storage tank 2 is used to prepare culture medium. Storage tank 2 includes a detachably connected tank body 21 and a cover 22, with a through hole on the cover 22. Stirrer 3 is used to stir the liquid culture medium in storage tank 2. Stirrer 3 includes a base 31, a fixed rod 32 fixed on the base 31, a support rod 33 that can slide up and down on the fixed rod 32, a motor 34 mounted on the support rod 33, a stirring shaft 35 connected to the motor 34, and multiple blades 36 fixed on the stirring shaft 35. The motor 34 can drive the stirring shaft 35 to rotate, and the stirring shaft 35 can pass through the through hole on the cover 22 and be placed inside the tank body 21. The motor 34 is electrically connected to a controller, which is used to control the start or stop of the motor 34. By setting the stirrer 3 to continuously stir the liquid culture medium in the storage tank, the uniform distribution of agar powder in the solution can be fully guaranteed, ensuring that the culture medium components dispensed into each tissue culture bottle 43 are equal in quantity.

[0024] like Figures 1-2 As shown, the operating table 1 is equipped with two slide rails 11. The dispensing assembly 4 includes a tray 41, which is mounted on the slide rails 11. An electric push rod 42 is connected to the tray 41, which can move the tray 41 on the slide rails 11. The tray 41 has five rows of placement slots, each row of which can hold ten tissue culture bottles 43. An n-type bracket 44 is also fixed on the operating table 1. The bracket 44 is located above the tray 41 and has ten mounting holes. A dispensing tube 45 can be detachably connected to each mounting hole. One end of the dispensing tube 45 is the liquid outlet, which is vertically downward and directly opposite the bottle opening of the tissue culture bottle 43. The other end of the dispensing tube 45 is the liquid inlet, which is connected to the inside of the storage tank 2. Each dispensing tube 45 is equipped with a water pump 46, a solenoid valve, and a flow meter. All components are electrically connected to the controller. The water pump 46 is used to extract liquid culture medium from the storage tank 2 and deliver it to the tissue culture bottle 43 through the dispensing tube 45. The flow meter is used to detect the liquid flow rate of the dispensing tube 45 and send a signal to the controller. The controller is used to control the start or stop of the water pump 46. The controller is also used to receive the signal from the flow meter and control the solenoid valve to open or close the fluid channel inside the dispensing tube 45 according to the signal. A sensing element 47 is installed on the inner side of the bracket 44. The sensing element 47 is electrically connected to the controller. The sensing element 47 is used to detect whether the tissue culture bottle 43 on the tray 41 has moved directly below the water outlet of the dispensing tube 45 and sends a signal to the controller. The controller receives the signal from the sensing element 47 and controls the start or stop of the electric push rod 42 and the water pump 46, as well as the opening or closing of the solenoid valve according to the signal.

[0025] By setting up a controller, water pump 46, solenoid valve and flow meter, the liquid output of dispensing tube 45 can be precisely controlled, so that the volume of liquid culture medium injected into each tissue culture bottle 43 is equal, realizing the automated quantitative dispensing of culture medium; by setting multiple tissue culture bottles 43 and dispensing tubes 45 on tray 41, batch dispensing of culture medium can be realized, and the operator does not need to hold the liquid storage tank during the dispensing process, avoiding hand burns. The dispensing operation is simple and fast, effectively speeding up the dispensing efficiency and reducing the labor intensity of the operator.

[0026] like Figures 1-2 As shown, a control panel 5 is also installed on the operating table 1. The control panel 5 is electrically connected to the controller. Through the control panel 5, commands can be sent to the controller to start or stop the motor 34, the electric push rod 42, and the water pump 46. The dispensing capacity of the culture bottle 43 can also be set.

[0027] The automated dispensing device of this embodiment dispenses culture media, including the following steps:

[0028] 1. Place 14.4g of MS culture medium raw materials, 30g of sucrose, 9g of agar, and 1L of distilled water into the container 21 of the storage container 2. After covering with the lid 22, adjust the position of the stirrer 3 so that the stirring shaft 35 passes through the through hole of the lid 22 and is placed inside the container 21.

[0029] 2. Send a command to start motor 34 to controller via control panel 5 so that stirring shaft 35 continuously stirs culture medium raw materials in storage tank 2 to make them evenly mixed, and place tissue culture bottle 43 into placement slot of tray 41.

[0030] 3. Set the dispensing capacity of the tissue culture bottle 43 through the control panel 5, and send a command to the controller to start the electric push rod 42. The electric push rod 42 moves the tray 41 until the sensing element 47 detects that the first row of tissue culture bottles 43 has moved directly below the water outlet of the dispensing tube 45. The controller controls the electric push rod 42 to stop, and starts the water pump 46 and opens the solenoid valve. The water pump 46 draws the liquid culture medium in the storage tank 2 into the tissue culture bottle 43 through the dispensing tube 45. After the flow meter detects that the liquid output of the dispensing tube 45 has reached the set capacity, the controller closes the solenoid valve and controls the electric push rod 42 to start again until the sensing element 47 detects that the next row of tissue culture bottles 43 has moved directly below the water outlet of the dispensing tube 45. Repeat the above operation until the liquid culture medium is dispensed. The controller controls the motor 34, electric push rod 42 and water pump 46 to stop running and closes the solenoid valve.

[0031] 4. After dispensing, put the cap on the tissue culture bottle 43 and place it in the basket. Put the basket and tissue culture bottle 43 into the autoclave for sterilization.

[0032] 5. After sterilization, remove the basket from the sterilizer and shake it from side to side to distribute the agar evenly in the culture medium (to prevent the agar from settling at the bottom during sterilization, which would result in a thin upper layer and a hard lower layer after natural condensation). Place the tissue culture bottle 43 in a container at 25°C for storage.

[0033] Traditional dispensing methods involve first sterilizing the culture medium at high temperature, and then dispensing it on a sterile workbench 1. The dispensing method described above, on the other hand, involves dispensing the culture medium first, and then sterilizing it. This greatly reduces the risk of contamination of the culture medium, and the dispensing operation does not require professional personnel, thus requiring less skill from the operators.

[0034] The above descriptions are merely embodiments of this utility model. Commonly known technical solutions and / or characteristics are not described in detail here. It should be noted that those skilled in the art can make various modifications and improvements without departing from the technical solution of this utility model. These modifications and improvements should also be considered within the scope of protection of this utility model, and will not affect the effectiveness of the implementation of this utility model or the practicality of the patent. The scope of protection claimed in this application should be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. An automated media aliquoting device, characterized by: The utility model provides a kind of tissue culture medium dispensing device, including operation platform (1) and be arranged on operation platform (1) storage barrel (2), agitator (3), partial shipment subassembly (4) and controller;Storage barrel (2) is used to prepare culture medium, agitator (3) is used to stir liquid medium in storage barrel (2);Partial shipment subassembly (4) includes tray (41) slidingly installed on operation platform (1), tray (41) is equipped with several rows of placement groove, each row of placement groove can place multiple tissue culture bottles (43);The upper of tray (41) is equipped with support (44), support (44) is equipped with multiple partial shipment pipes (45), one end of partial shipment pipe (45) is liquid outlet end, liquid outlet end vertically downward and directly opposite the bottle mouth of tissue culture bottle (43), the other end of partial shipment pipe (45) is liquid inlet end, and liquid inlet end is communicated with inside of storage barrel (2), and water pump (46) is equipped on each partial shipment pipe (45), and water pump (46) is used to extract liquid medium in storage barrel (2) and is delivered to tissue culture bottle (43) by partial shipment pipe (45);Water pump (46) is electrically connected with controller, and controller is used to control the start or stop of water pump (46).

2. The automated media dispensing apparatus of claim 1, wherein: The partial shipment pipe (45) is also equipped with solenoid valve and flowmeter, and the solenoid valve and the flowmeter are electrically connected with the controller, and the flowmeter is used to detect the liquid flow of the partial shipment pipe (45) and send a signal to the controller, and the controller is used to receive the signal of the flowmeter and control the opening and closing of the solenoid valve.

3. The automated media dispensing apparatus of claim 2, wherein: The operation platform (1) is equipped with a sliding rail (11), and the tray (41) is installed on the sliding rail (11), and the tray (41) is connected with an electric push rod (42), and the electric push rod (42) can drive the tray (41) to move on the sliding rail (11), and the electric push rod (42) is electrically connected with the controller, and the controller is used to control the start or stop of the electric push rod (42).

4. The automated media dispensing apparatus of claim 3, wherein: The inner side of the support (44) is equipped with an inductive element (47) electrically connected with the controller, and the inductive element (47) is used to detect whether the tissue culture bottle (43) on the tray (41) moves to the directly below of the water outlet end of the partial shipment pipe (45) and sends a signal to the controller, and the controller is used to receive the signal of the inductive element (47) and control the start or stop of the electric push rod (42) and the water pump (46) according to the signal, and control the opening and closing of the solenoid valve.

5. The automated media dispensing apparatus of claim 4, wherein: The agitator (3) includes a base (31), and the base (31) is equipped with a fixed rod (32), and the fixed rod (32) is equipped with a slidable support rod (33), and the support rod (33) is installed with a motor (34), and the motor (34) is connected with an agitator shaft (35), and the agitator shaft (35) is equipped with a plurality of blades (36), and the motor (34) can drive the agitator shaft (35) to rotate;The motor (34) is electrically connected with the controller, and the controller can control the start and stop of the motor (34).

6. The automated media dispensing apparatus of claim 5, wherein: The storage barrel (2) includes a detachably connected barrel body (21) and a cover body (22), and the cover body (22) is equipped with a through hole, and the agitator shaft (35) can pass through the through hole and be placed in the barrel body (21).

7. The automated media dispensing apparatus of claim 5, wherein: The operation platform (1) is provided with a control panel (5), the control panel (5) is electrically connected with the controller, instructions of starting or stopping the motor (34), the electric push rod (42) and the water pump (46) can be sent to the controller through the control panel (5), and the split quantity of the tissue culture bottle (43) can be set.

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