Stem cell PCR instrument heat conduction plate and PCR instrument

Abstract

The utility model discloses a heat conduction plate of a stem cell PCR instrument and the PCR instrument, and relates to the field of PCR instruments, the heat conduction plate comprises a sliding frame, one side of the sliding frame is fixedly connected with a pulling block, and the sliding frame is internally provided with a fixing mechanism, through the structural arrangement, the heat conduction plate of the stem cell PCR instrument and the PCR instrument can drive a third rack to vertically move by means of vertical movement of a carrying plate, so that the heat conduction plate is convenient to move; a gear II is driven by a rack III to rotate so as to drive a cover plate to rotate, so that a top heating module on the side wall of the cover plate is attached to the top of the carrying plate, the top and the bottom of the carrying plate simultaneously conduct heat to the carrying plate through the top heating module and a bottom heating module, and heat transfer is more uniform during detection.

Description

TECHNICAL FIELD

[0001] The application relates to the field of PCR instruments, in particular to a stem cell PCR instrument heat-conducting plate and a PCR instrument. BACKGROUND

[0002] The stem cell PCR instrument is an application of a PCR instrument in the field of stem cell research. The stem cell PCR instrument is an experimental instrument for polymerase chain reaction (PCR) in stem cell research, which realizes DNA denaturation, annealing and extension by precisely controlling temperature.

[0003] As shown in a PCR instrument heat-conducting plate and a PCR instrument disclosed in Chinese Patent Publication No. CN220413328U, the heat-conducting plate body, the first matrix hole group, the second matrix hole group and the third matrix hole group. The first matrix hole group includes n first holes, the second matrix hole group includes m second holes, the first matrix hole group and the second matrix hole group are arranged in a matrix on the heat-conducting plate body, n is a multiple of m, each second hole intersects and communicates with the adjacent a first holes to form a hole group unit, and the m hole group units are arranged in a matrix on the heat-conducting plate body. In actual use, the following problems still exist:

[0004] The stem cell PCR instrument heat-conducting plate and the PCR instrument of the above patent are installed by unit recombination in actual use, but the heat conduction is performed by the heat-conducting glue at the bottom of the PCR instrument, so that the temperature is gradually transmitted from the bottom to the top in the actual heating process, the heating of the upper and lower ends of the sample is uneven, and the detection data is inaccurate. Content of the utility model

[0005] In order to improve the problem of uneven heating, the application provides a stem cell PCR instrument heat-conducting plate and a PCR instrument.

[0006] The stem cell PCR instrument heat-conducting plate and the PCR instrument provided by the application adopt the following technical scheme:

[0007] A stem cell PCR instrument heat-conducting plate, comprising a sliding frame, a pulling block is fixedly connected to one side of the sliding frame, and a fixing mechanism is arranged in the sliding frame;

[0008] The fixing mechanism comprises a gear one, a knob is arranged at the top of the gear one, a rack one is movably arranged on the side wall of the gear one, a rack two is movably arranged on the side wall of the gear one, a sliding rod is fixedly arranged at the top of the rack one, a sliding rod is fixedly arranged on one side of the rack two, a connecting rod is movably arranged through the side wall of the sliding rod, a carrying plate is movably arranged at the end of the connecting rod away from the sliding rod, a rack three is fixedly arranged on the side wall of the carrying plate, a gear two is movably arranged on the side wall of the rack three, a cover plate is arranged on one side of the gear two, a top heating module is fixedly arranged on the side wall of the cover plate, and a bottom heating module is arranged at the top of the carrying plate.

[0009] By adopting the technical scheme, the rack two is placed in parallel with the rack one, so that the movement of both sides is more coherent, and the top of the rack one and the rack two is attached to the bottom of the support plate, so that the rack one and the rack two are limited by the support plate.

[0010] Preferably, the fixing mechanism further comprises a support block fixedly connected to the top of the sliding frame, a support plate is fixedly connected to the side wall of the support block, a rotating shaft is movably penetrated through the top of the support plate and fixedly penetrated through the top of the gear one, the top of the rotating shaft is fixedly connected to the bottom of the knob, the side wall of the gear one is meshingly connected to the side of the rack one close to the gear one, and the side wall of the gear one is meshingly connected to the side of the rack two close to the gear one.

[0011] By adopting the technical scheme, the support plate is fixedly connected to the support block on both sides, so that the support plate and the sliding frame are in a parallel state.

[0012] Preferably, the side of the rack two away from the gear one is attached to a limiting plate one fixedly connected to the top of the sliding frame, one end of the rack two away from the gear one is fixedly connected to a connecting strip, and one end of the connecting strip away from the rack two is fixedly connected to a connecting plate one fixedly penetrated through the sliding rod.

[0013] By adopting the technical scheme, the connecting plate one and the connecting plate two are in the same horizontal straight line, so that the movement of both sides is more synchronized.

[0014] Preferably, the top of the rack one is fixedly connected to a connecting plate two fixedly penetrated through the sliding rod, the side wall of the sliding rod is movably penetrated through a limiting plate two fixedly connected to the sliding frame, the side wall of the connecting rod is movably penetrated through the sliding rod, and one end of the connecting rod away from the sliding rod is movably penetrated through a rotating shaft.

[0015] By adopting the technical scheme, the limiting plate two is symmetrically arranged, so that the support of the sliding rod is more stable.

[0016] Preferably, the side wall of the rotating shaft is movably penetrated through a connecting piece fixedly connected to the bottom of the mounting plate, the top of the mounting plate is fixedly connected to a baffle, the side wall of the mounting plate is fixedly connected to a rack three, and the side of the rack three close to the gear two is meshingly connected to the gear two.

[0017] By adopting the technical scheme, the baffle is arranged at four diagonal positions on the top of the mounting plate, so as to stabilize the sample on the top.

[0018] Preferably, the side wall of the gear two is fixedly penetrated through a connecting column, the side wall of the connecting column is fixedly penetrated through a rotating piece, the side wall of the rotating piece is fixedly connected to a cover plate, and the side wall of the cover plate is fixedly penetrated through a top heating module.

[0019] By adopting the technical scheme, the cover plates are symmetrically arranged, thereby completely covering the carrying plate through both sides.

[0020] Preferably, one end of the connecting column is movably penetrated through the side wall of the pulling block, and the other end of the connecting column is movably penetrated through the inner side wall of the sliding frame, and the inner wall bottom of the sliding frame is fixedly connected with the bottom heating module.

[0021] By adopting the technical scheme, the area of the bottom heating module is smaller than the area of the bottom of the carrying plate, and the geometric center of the bottom heating module is kept in line with the carrying plate, thereby enabling the bottom heating module to diffusively heat the carrying plate.

[0022] A PCR instrument is applied to the heat-conducting plate of the stem cell PCR instrument, and comprises an instrument main body, the sliding frame is slidably arranged in the instrument main body and can move to the outside of the instrument main body, and the instrument main body is clamped with the pulling block.

[0023] By adopting the technical scheme, the outer wall of the pulling block can be completely clamped with the outer wall of the instrument main body, thereby making the clamping more stable.

[0024] In summary, the present application has at least one of the following beneficial technical effects:

[0025] 1. The knob drives the gear to rotate, thereby driving the rack one and the rack two to move in opposite directions, thereby driving the sliding rod to move horizontally, the sliding rod gradually changes the connecting rod from the inclined state to the horizontal state, thereby driving the carrying plate to move downward, so that the carrying plate is separated from the bottom heating module when the stem cell element is placed, thereby avoiding the bottom heating module from heating the carrying plate when the element is placed, and the vertical movement of the carrying plate also facilitates the staff to take out or place the element placed on the top of the carrying plate.

[0026] 2. The vertical movement of the carrying plate drives the rack three to move vertically, and then the rack three drives the gear two to rotate, thereby driving the cover plate to rotate, so that the top heating module of the side wall of the cover plate is attached to the top of the carrying plate, thereby the top and bottom of the carrying plate are simultaneously heat-conducted by the top heating module and the bottom heating module, thereby making the heat transfer more uniform during detection. BRIEF DESCRIPTION OF DRAWINGS

[0027] Figure 1 It is a whole structure display diagram of the heat-conducting plate and the PCR instrument of the stem cell PCR instrument.

[0028] Figure 2 It is a local display diagram of the knob of the heat-conducting plate and the PCR instrument of the stem cell PCR instrument.

[0029] Figure 3Figure 6 is a partial cross-sectional view of the heat-conducting plate and the rotating shaft of the stem cell PCR instrument of the present application;

[0030] Figure 4 Figure 7 is a partial view of the heat-conducting plate and the connecting strip of the stem cell PCR instrument of the present application;

[0031] Figure 5 Figure 8 is a partial view of the heat-conducting plate and the connecting rod of the stem cell PCR instrument of the present application.

[0032] Reference signs:

[0033] 1, instrument main body; 11, sliding frame; 12, pull-out block;

[0034] 2, fixing mechanism; 21, support block; 22, support plate; 23, rotating shaft; 24, gear one; 25, knob; 26, rack one; 27, rack two; 28, limiting plate one; 29, connecting strip; 210, connecting plate one; 211, connecting plate two; 212, sliding rod; 213, limiting plate two; 214, connecting rod; 215, rotating shaft; 216, connecting piece;

[0035] 217, mounting plate; 218, baffle; 219, rack three; 220, gear two; 221, connecting column; 222, rotating piece; 223, cover plate; 224, top heating module; 225, bottom heating module. DETAILED DESCRIPTION

[0036] The following will be described in detail in combination with the accompanying drawings Figures 1-5 The present application will be further described in detail.

[0037] The present application discloses a kind of stem cell PCR instrument heat-conducting plate.

[0038] Example 1

[0039] Refer to Figure 1 A kind of stem cell PCR instrument heat-conducting plate, including sliding frame 11, pull-out block 12 is fixedly connected to one side of sliding frame 11, sliding frame 11 inside is provided with fixing mechanism 2.

[0040] Staff will be placed on the desktop of sliding frame 11, prepare to carry out sample.

[0041] Refer to Figure 2The fixing mechanism 2 comprises a gear one 24, a knob 25 arranged on the top of the gear one 24, a rack one 26 movably arranged on the side wall of the gear one 24, a rack two 27 movably arranged on the side wall of the rack one 26, a sliding rod 212 fixedly arranged on the top of the rack one 26, the sliding rod 212 fixedly arranged on one side of the rack two 27, a connecting rod 214 movably penetrating the side wall of the sliding rod 212, a carrying plate 217 movably arranged on the end of the connecting rod 214 away from the sliding rod 212, a rack three 219 fixedly arranged on the side wall of the carrying plate 217, a gear two 220 movably arranged on the side wall of the rack three 219, a cover plate 223 arranged on one side of the gear two 220, a top heating module 224 fixedly arranged on the side wall of the cover plate 223, and a bottom heating module 225 arranged on the top of the carrying plate 217. The fixing mechanism 2 further comprises a supporting block 21 fixedly connected with the top of the sliding frame 11, a supporting plate 22 fixedly connected with the side wall of the supporting block 21, a circular hole one penetratingly arranged on the top of the supporting plate 22, a rotating shaft 23 movably penetrating the circular hole one, so that the rotating shaft 23 is rotatably connected with the supporting plate 22, a circular hole two penetratingly arranged on the top of the gear one 24, and the rotating shaft 23 fixedly penetrating the top of the gear one 24, so that the rotating shaft 23 is fixedly connected with the gear one 24.

[0042] Then the staff places the sample on the top of the carrying plate 217, and then rotates the knob 25, so that the knob 25 drives the rotating shaft 23 to rotate, the rotating shaft 23 drives the gear one 24 to rotate, the gear one 24 drives the rack one 26 and the rack two 27 to move horizontally away from the knob 25, the rack one 26 drives the connecting plate two 211 to move synchronously, and the rack two 27 drives one side of the connecting strip 29 to move horizontally.

[0043] Referring to Figure 3The bottom of the knob 25 is fixedly connected to the top of the rotating shaft 23. The gear rack one 26 is meshingly connected to the side wall teeth of the gear one 24 on one side close to the gear one 24. The gear rack two 27 is meshingly connected to the side wall teeth of the gear one 24 on one side close to the gear one 24. The gear rack two 27 is in abutment with the limiting plate one 28 on one side away from the gear one 24. The gear rack one 26 is in abutment with the limiting plate one 28 on one side away from the gear one 24. Therefore, the gear rack two 27 and the gear rack one 26 are limited by the limiting plate one 28. The bottom of the limiting plate one 28 is fixedly connected to the top of the sliding frame 11. The connecting strip 29 is fixedly connected to one end of the gear rack two 27 away from the gear one 24. The connecting plate one 210 is fixedly connected to one end of the connecting strip 29 away from the gear rack two 27. The side wall of the connecting plate one 210 is provided with a round hole three. The side wall of the sliding rod 212 is fixedly penetrated through the round hole three, so that the sliding rod 212 is fixedly penetrated through the connecting plate one 210. The connecting plate two 211 is fixedly connected to the top of the gear rack one 26. The side wall of the connecting plate two 211 is provided with a round hole four. The side wall of the sliding rod 212 is fixedly penetrated through the round hole four, so that the sliding rod 212 is fixedly connected to the connecting plate two 211. The side wall of the limiting plate two 213 is provided with a horizontal sliding slot. The side wall of the sliding rod 212 is movably penetrated through the horizontal sliding slot, so that the sliding rod 212 is movably connected to the limiting plate two 213.

[0044] One end of the connecting strip 29 away from the gear rack two 27 will drive the connecting plate one 210 to move synchronously, so that the connecting plate one 210 and the connecting plate two 211 drive the sliding rods 212 on both sides to move horizontally synchronously to both sides. The sliding rod 212 will pull the connecting rod 214 from the inclined state to the horizontal state, so that the connecting rod 214 pulls the carrying plate 217 to move vertically downward to the bottom of the carrying plate 217 abutting against the top of the bottom heating module 225.

[0045] Referring to Figure 4 The bottom of the limiting plate two 213 is fixedly connected to the top of the inner wall of the sliding frame 11, so that the limiting plate two 213 is fixedly connected to the sliding frame 11. The side wall of one end of the connecting rod 214 close to the sliding rod 212 is provided with a round hole five. The sliding rod 212 movably penetrates through the round hole five, so that the sliding rod 212 is rotatably connected to the connecting rod 214. The connecting rod 214 is located between the two limiting plates two 213, so that the two limiting plates two 213 limit the connecting rod 214. The side wall of one end of the connecting rod 214 away from the sliding rod 212 is provided with a round hole six. The rotating shaft 215 movably penetrates through the round hole six, so that the rotating shaft 215 is rotatably connected to the connecting rod 214. The side wall of the connecting piece 216 is provided with a round hole seven. The rotating shaft 215 movably penetrates through the round hole seven, so that the rotating shaft 215 is rotatably connected to the connecting piece 216. The top of the connecting piece 216 is fixedly connected to the bottom of the carrying plate 217. The baffle 218 is fixedly connected to the top of the carrying plate 217.

[0046] As the mounting plate 217 moves downward vertically, it will also drive the rack 3 219 to move downward vertically. The rack 3 219 will drive the gear 220 to rotate, the gear 220 will drive the connecting column 221 to rotate, and the connecting column 221 will drive the rotating component 222 to rotate.

[0047] Reference Figure 5 Rack 3 219 is fixedly connected to the side wall of mounting plate 217. Gear 2 220 is meshed with rack 3 219 on the side near gear 2 220. Gear 2 220 has a through hole 8 in its side wall. Connecting post 221 is fixedly connected to gear 2 220 through hole 8. Rotating component 222 has a through hole 9 in its side wall. Connecting post 221 is fixedly connected to rotating component 222 through hole 9. Cover plate 223 is fixedly connected to rotating component 222. 2. The side wall of the pull-out block 12 has a circular hole nine. One end of the connecting post 221 is movably connected to the circular hole nine, so that the connecting post 221 and the pull-out block 12 are rotatably connected. The inner side wall of the sliding frame 11 has a circular hole ten. The other end of the connecting post 221 is movably connected to the circular hole ten, so that the sliding frame 11 and the connecting post 221 are rotatably connected. The side wall of the cover plate 223 has a square slot. The top heating module 224 is fixedly connected through the square slot. The bottom heating module 225 is fixedly connected to the bottom of the inner wall of the sliding frame 11.

[0048] When the rotating component 222 rotates, it will drive the cover plate 223 to rotate synchronously, thereby changing the cover plate 223 from a vertical state to a horizontal state. This allows the top heating module 224 to be positioned on top of the sample mounted on the mounting plate 217, thus conducting heat to the sample from the top through the top heating module 224.

[0049] The top heating module 224 and the bottom heating module 225 are existing technologies, and their structural principles will not be described in detail. They also include power connection wires, power switches, control panels, etc., which are not the main technologies and will not be described in detail.

[0050] The implementation principle of the heat-conducting plate of the stem cell PCR instrument in this application embodiment is as follows:

[0051] The staff places the sample on top of the mounting plate 217, and then rotates the knob 25. The knob 25 drives rack 1 26 and rack 2 27 to move horizontally away from the knob 25. Through connecting plate 1 210 and connecting plate 211, the sliding rods 212 on both sides move horizontally to both sides simultaneously. The sliding rods 212 pull the connecting rod 214 from an inclined state to a horizontal state, so that the bottom of the mounting plate 217 is in contact with the top of the bottom heating module 225. While the mounting plate 217 moves vertically downward, it also drives rack 3 219 to move vertically downward. Rack 3 219 drives the cover plate 223 to rotate synchronously, so that the cover plate 223 changes from a vertical state to a horizontal state. Thus, the cover plate 223 drives the top heating module 224 to be located on top of the sample mounted on the mounting plate 217, so that the sample is heated from the top through the top heating module 224.

[0052] Example 2

[0053] Reference Figure 1 The difference between this embodiment and embodiment 1 is that a PCR instrument includes an instrument body 1, a sliding frame 11 is slidably connected inside the instrument body 1 and can be pulled out by pulling the pull block 12, and the inner wall of the instrument body 1 and the outer wall of the pull block 12 are engaged.

[0054] The main body of the instrument 1 is existing technology, and its structural principle will not be described in detail.

[0055] The implementation principle of a PCR instrument in this application embodiment is as follows:

[0056] After the sample is placed, the staff aligns the sliding frame 11 with the opening on the side wall of the instrument body 1, and then slides the sliding frame 11 to one side of the instrument body 1 so that the sliding frame 11 slides into the instrument body 1 and the pull block 12 engages with the instrument body 1. Then the instrument body 1 is started to test the sample inside the sliding frame 11.

[0057] The above are merely optional embodiments of this disclosure and are not intended to limit this disclosure. Various modifications and variations can be made to this disclosure by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this disclosure should be included within the scope of protection of this disclosure.

Claims

1. A heat-conducting plate for a stem cell PCR instrument, comprising a sliding frame (11), wherein a pull block (12) is fixedly connected to one side of the sliding frame (11), characterized in that: The sliding frame (11) is equipped with a fixing mechanism (2); The fixing mechanism (2) includes a gear one (24), a knob (25) on the top of the gear one (24), a rack one (26) movably mounted on the side wall of the gear one (24), a rack two (27) movably mounted on the side wall of the rack one (26), a sliding rod (212) fixedly mounted on the top of the rack one (26), a sliding rod (212) fixedly mounted on one side of the rack two (27), and a connecting rod (214) movably passing through the side wall of the sliding rod (212). A mounting plate (217) is movably provided at one end of the connecting rod (214) away from the sliding rod (212). A rack three (219) is fixedly provided on the side wall of the mounting plate (217). A gear two (220) is movably provided on the side wall of the rack three (219). A cover plate (223) is provided on one side of the gear two (220). A top heating module (224) is fixedly provided on the side wall of the cover plate (223). A bottom heating module (225) is provided on the top of the mounting plate (217).

2. The heat-conducting plate for a stem cell PCR instrument according to claim 1, characterized in that: The fixing mechanism (2) also includes a support block (21) fixedly connected to the top of the sliding frame (11). A support plate (22) is fixedly connected to the side wall of the support block (21). A rotating shaft (23) is movably passed through the top of the support plate (22) and fixedly passed through the top of the gear one (24). The top of the rotating shaft (23) is fixedly connected to the bottom of the knob (25). The side wall of the gear one (24) is meshed with the side of the rack one (26) near the gear one (24). The side wall of the gear one (24) is meshed with the side of the rack two (27) near the gear one (24).

3. The heat-conducting plate for a stem cell PCR instrument according to claim 2, characterized in that: The side of the rack two (27) away from the gear one (24) is fitted with a limiting plate one (28) that is fixedly connected to the top of the sliding frame (11). The end of the rack two (27) away from the gear one (24) is fixedly connected with a connecting strip (29). The end of the connecting strip (29) away from the rack two (27) is fixedly connected with a connecting plate one (210) that is fixedly connected to the sliding rod (212).

4. The heat-conducting plate for a stem cell PCR instrument according to claim 3, characterized in that: The top of the rack (26) is fixedly connected to the connecting plate (211) that is fixedly connected to the sliding rod (212). The side wall of the sliding rod (212) is movably connected to the limiting plate (213) that is fixedly connected to the sliding frame (11). The side wall of the connecting rod (214) is movably connected to the sliding rod (212). The end of the connecting rod (214) away from the sliding rod (212) is movably connected to the rotating shaft (215).

5. The heat-conducting plate for a stem cell PCR instrument according to claim 4, characterized in that: The rotating shaft (215) has a connecting piece (216) that is fixedly connected to the bottom of the mounting plate (217) through its side wall. The mounting plate (217) has a baffle (218) fixedly connected to its top. The side wall of the mounting plate (217) is fixedly connected to the rack three (219). The rack three (219) is meshed with the gear two (220) on the side near the gear two (220).

6. The heat-conducting plate for a stem cell PCR instrument according to claim 5, characterized in that: A connecting column (221) is fixedly inserted through the side wall of the gear two (220), a rotating component (222) is fixedly inserted through the side wall of the connecting column (221), a cover plate (223) is fixedly connected to the side wall of the rotating component (222), and a top heating module (224) is fixedly inserted through the side wall of the cover plate (223).

7. The heat-conducting plate for a stem cell PCR instrument according to claim 6, characterized in that: One end of the connecting column (221) is movably connected to the side wall of the pull block (12), and the other end of the connecting column (221) is movably connected to the inner side wall of the sliding frame (11). The bottom of the inner wall of the sliding frame (11) is fixedly connected to the bottom of the bottom heating module (225).

8. A PCR instrument, applied to the heat-conducting plate of a stem cell PCR instrument according to any one of claims 1-7, characterized in that: The instrument includes a main body (1), and the sliding frame (11) is slidably disposed inside the main body (1) and can be moved to the outside of the main body (1). The main body (1) and the pull block (12) are engaged.

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