Chromatographic separation equipment

Abstract

The utility model relates to the technical field of medical instruments, and discloses chromatographic separation equipment which comprises a base, a separation mechanism is arranged in the base and comprises a plurality of springs, a connecting block, a rotating block, a rack, a rotating column, a gear and a motor, and a moving frame is arranged at the upper end of the base in a sliding mode. A separation box is fixedly arranged among the upper ends of the multiple springs, a protection pad is fixedly arranged in the separation box, and multiple sealing mechanisms are arranged in the protection pad; the plurality of sealing mechanisms comprise a plurality of sealing covers, a plurality of first separation pipes, a plurality of sealing gaskets, a plurality of threaded elastic sheets, a plurality of rotating rings, a plurality of fixed rings, a plurality of second separation pipes, a plurality of third separation pipes, a plurality of sealing rings and a plurality of filter membranes. According to the utility model, when the device is used, the driving motor drives the moving frame to slide on the base, and the separating box in the moving frame vibrates, so that the separating efficiency of similar cells in the chromatography process is improved.

Description

Technical Field

[0001] This utility model relates to the field of medical device technology, and in particular to a chromatography separation device. Background Technology

[0002] Organoids are three-dimensional cell cultures, containing some key characteristics of their representative organs. Chromatography, as a highly efficient separation technique, is based on the significant differences in the physicochemical properties of different substances. By constructing a system composed of a stationary phase and a mobile phase, when the complex mixture to be separated is introduced and slowly flows through the stationary phase with the mobile phase, each component exhibits different affinities with the two phases due to its unique physicochemical properties, such as adsorption, dissolution, and specific binding. This differential interaction leads to different distribution ratios of each component between the two phases, that is, their content ratios in the stationary and mobile phases are significantly different. As the mobile phase continues to push, the components in the mixture undergo a continuous redistribution process between the two phases, gradually separating the originally mixed components in time and space. Therefore, chromatography has become an indispensable tool in fields such as biochemistry, molecular biology, and drug development.

[0003] Existing patent (publication number: CN219156875U) discloses "an organoid chromatography separation device, characterized in that it includes: a base, a housing fixedly mounted on the top of the base, a servo motor fixedly mounted on the inner wall of the housing, a rotating shaft fixedly mounted on the output shaft of the servo motor, the rotating shaft being rotatably connected to the housing, a bottom plate and a top plate fixedly sleeved on the rotating shaft, multiple support grooves formed on the bottom plate, multiple inclined grooves formed on the top plate, a mounting seat fixedly mounted on the top of the top plate, a protective cover threaded onto the mounting seat, a first buffer pad provided on the inner wall of the protective cover, second buffer pads provided on the inner walls of the multiple support grooves, and multiple separation structures, all of which are located on the top of the bottom plate. This utility model..." The beneficial effects of this design are as follows: the servo motor can drive the rotating shaft, bottom plate, and top plate to rotate, thereby centrifuging and classifying the cell fluid in multiple separation structures; the protective cover and the first buffer pad can protect the top of multiple separation structures; the second buffer pads can protect the bottom of multiple separation structures; the first separation tube, the second separation tube, and the third separation tube can store the separated cell fluid; the sealing cover can seal the third separation tube; the two annular plates can support the two filter membranes; the two filter membranes can separate organoid tissues in the cell fluid into three different sizes; the three sealing rings can improve the sealing between the first separation tube, the second separation tube, and the third separation tube; and the controller can control the servo motor.

[0004] In the process of developing this application, the inventors discovered the following problems with the prior art:

[0005] 1. Although the device uses sealing rings to improve sealing, the performance of the sealing rings may be affected during high-speed rotation, which may lead to sample leakage and affect sample safety and separation efficiency.

[0006] 2. This device uses a motor to drive the rotating shaft, bottom plate and top plate to rotate, so as to achieve centrifugal separation of cell fluid in multiple separation structures. Due to the centrifugal separation method, the separation efficiency is low.

[0007] Therefore, those skilled in the art have provided a chromatographic separation apparatus to solve the problems mentioned in the background art. Utility Model Content

[0008] The purpose of this invention is to address the shortcomings of existing technologies by proposing a chromatography separation device that uses a separation mechanism to classify cell-like structures in cell fluid through vibration, and uses a sealing mechanism to improve the sealing between multiple separation tubes.

[0009] To achieve the above objectives, the present invention provides the following technical solution:

[0010] A chromatography separation device includes a base, and a separation mechanism is provided inside the base. The separation mechanism includes multiple springs, connecting blocks, rotating blocks, racks, rotating columns, gears and motors. A movable frame is slidably provided on the upper end of the base, and a separation box is fixedly provided between the upper ends of the multiple springs.

[0011] The separation box is fixedly equipped with a protective pad, and the protective pad is equipped with multiple sealing mechanisms. The multiple sealing mechanisms include multiple sealing caps, multiple first separation tubes, multiple sealing pads, multiple threaded springs, multiple rotating rings, multiple fixed rings, multiple second separation tubes, multiple third separation tubes, multiple sealing rings, and multiple filter membranes.

[0012] Furthermore, the lower ends of the multiple springs are all fixedly disposed inside the lower end of the movable frame, the external rotating column is rotatably disposed inside the lower end of the movable frame, and the internal gear is fixedly disposed outside the rotating column.

[0013] Furthermore, the lower end of the rack is fixedly mounted on the upper end of the base, the gear is externally meshed with the upper end of the rack, and the separation box is externally slidably mounted inside the movable frame.

[0014] Furthermore, the motor is externally fixedly mounted inside the base on one side, the rotating block is fixedly mounted on one side of the motor output end, the front end of the connecting block is rotatably mounted on one side of the rotating block near the rear end, and the moving frame is rotatably mounted on one side of the connecting block.

[0015] Furthermore, the interior of the plurality of sealing caps is respectively threaded onto the upper outer side of the plurality of first separation tubes, the exterior of the plurality of filter membranes is respectively fixedly disposed inside the plurality of second separation tubes and the plurality of third separation tubes, the interior of the plurality of fixing rings is respectively fixedly disposed on the upper outer side of the plurality of second separation tubes and the plurality of third separation tubes, the lower end of the plurality of threaded springs is respectively fixedly disposed on the upper end of the plurality of fixing rings, and the interior of the plurality of rotating rings is respectively threaded onto the exterior of the plurality of threaded springs and the plurality of fixing rings.

[0016] Furthermore, multiple sealing gaskets are fixedly mounted on opposite sides of multiple threaded springs on opposite sides of each other. Multiple sealing rings are fixedly mounted on the upper part of the interior of multiple second separation tubes and multiple third separation tubes. The lower ends of multiple first separation tubes are threaded onto the upper ends of multiple second separation tubes, and the lower ends of multiple second separation tubes are threaded onto the upper ends of multiple third separation tubes.

[0017] Furthermore, four anti-slip pads are fixedly installed at the lower end of the base, a control panel is fixedly installed on one side of the front end of the base, a hinged cover is hinged to the upper end of the separation box, and a buffer pad is fixedly installed at the lower end of the hinged cover.

[0018] This utility model has the following beneficial effects:

[0019] 1. The present invention proposes a chromatography separation device in which, during separation, cell fluid containing cell-like organisms is poured into a first separation tube. Then, a sealing cap, a second separation tube, and a third separation tube are threaded together, causing the sealing ring to be compressed, thereby improving the sealing performance of the separation tube. Then, rotating the rotating ring causes the threaded spring to contract inward, thereby making the sealing gasket tightly fit against the outer wall of the separation tube, further enhancing the sealing performance of the separation tube. At the same time, it can improve the connection stability between the separation tubes. When the separation chamber moves up and down, the cell-like organisms in the first separation tube inside it begin to pass through the filter membranes with different pore sizes preset in the second and third separation tubes, so that organoid tissues of different sizes are effectively trapped in the corresponding separation tubes, improving the separation efficiency and preventing sample leakage during the separation process, thereby ensuring the integrity of the sample.

[0020] 2. The chromatography separation device proposed in this utility model separates the cells inside the separation tube by starting the motor to rotate the rotating block at its output end. The rotation of the rotating block will drive the connecting block to push the moving frame to slide on the base. Since the gear and rack outside the rotating column mesh, as the moving frame moves, the gear will drive the rotating column to rotate, so that the rotating column drives the separation box to slide up and down reciprocally inside the moving frame. The spring at the lower end of the separation box will improve the separation effect, so that the cells in the first separation tube can pass through filter membranes of different pore sizes to achieve effective classification and separation, thereby improving the separation efficiency. Attached Figure Description

[0021] Figure 1 This is an isometric schematic diagram of the present invention;

[0022] Figure 2 This is a bottom-view orthographic section schematic diagram of the movable frame of this utility model;

[0023] Figure 3 This is a bottom-view orthographic section schematic diagram of the present invention, close to the movable frame;

[0024] Figure 4 This is a partial isometric view of the separation box of this utility model in the open state;

[0025] Figure 5 This is a rear-view isometric schematic diagram of the present invention, close to the motor;

[0026] Figure 6 This is a partial isometric view of the sealing mechanism of this utility model;

[0027] Figure 7 This is a partial orthographic isometric view of the second separation tube of this utility model.

[0028] Legend:

[0029] 1. Hinge cover; 2. Moving frame; 3. Separation mechanism; 4. Base; 5. Control panel; 6. Separation box; 7. Anti-slip pad; 8. Protective pad; 9. Sealing mechanism; 10. Buffer pad; 301. Spring; 302. Connecting block; 303. Rotating block; 304. Rack; 305. Rotating column; 306. Gear; 307. Motor; 901. Sealing cover; 902. First separation tube; 903. Sealing gasket; 904. Threaded spring; 905. Rotating ring; 906. Fixed ring; 907. Second separation tube; 908. Third separation tube; 909. Sealing ring; 910. Filter membrane. Detailed Implementation

[0030] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0031] Reference Figure 1-7 One embodiment provided by this utility model:

[0032] A chromatography separation device includes a base 4, inside which a separation mechanism 3 is disposed. The separation mechanism 3 includes multiple springs 301, a connecting block 302, a rotating block 303, a rack 304, a rotating column 305, a gear 306, and a motor 307. A movable frame 2 is slidably disposed on the upper end of the base 4. A separation box 6 is fixedly disposed between the upper ends of the multiple springs 301. The lower ends of the multiple springs 301 are all fixedly disposed inside the lower end of the movable frame 2. The rotating column 305 is rotatably disposed outside the lower end of the movable frame 2. The gear 306 is fixedly disposed inside the outside of the rotating column 305. The lower end of the rack 304 is fixedly mounted on the upper end of the base 4. The gear 306 is externally meshed with the upper end of the rack 304. The separation box 6 is externally slidably mounted inside the moving frame 2. The motor 307 is externally fixedly mounted inside the base 4 on one side. The rotating block 303 is fixedly mounted on one side of the output end of the motor 307. The front end of the connecting block 302 is rotatably mounted on one side of the rotating block 303 on the other side of the rear end. The moving frame 2 is rotatably mounted on one side of the connecting block 302. Four anti-slip pads 7 are fixedly mounted on the lower end of the base 4. A control panel 5 is fixedly mounted on one side of the front end of the base 4.

[0033] Specifically, when separating the cell-like structures inside the separation tube, the separation tube is first inserted into the protective pad 8. Then, the hinge cover 1 is closed, causing the buffer pad 10 at its lower end to press against the separation tube, thereby fixing it in place. Next, the motor 307 inside the base 4 is activated via the control panel 5, causing the output end of the motor 307 to rotate. This causes the rotating block 303 to rotate, which in turn causes the connecting block 302 to rotate, thus causing the moving frame 2 to reciprocate horizontally on the base 4. Simultaneously, a gear 306 is fixedly installed on the outside of the rotating column 305 inside the moving frame 2. This gear 306 is meshed with a rack 304 fixed on the base 4. As the moving frame... As the frame moves horizontally, gear 306 rolls along rack 304, causing rotating column 305 to rotate inside the moving frame 2. The rotation of rotating column 305 causes separation box 6 to reciprocate up and down inside. Spring 301 connected to the bottom of separation box 6 will undergo elastic deformation during the up and down movement of separation box 6, increasing the vibration frequency and thus enhancing the separation effect of cell-like structures inside separation box 6. This allows the cells in the first separation tube 902 to be subjected to the dual effects of vibration and filter membrane 910, achieving the effect of cell classification. The filter membrane 910 has filtration pore sizes of 100 micrometers and 250 micrometers, which can screen and classify different types of cells according to their pore size, ensuring the accuracy and efficiency of separation.

[0034] A protective pad 8 is fixedly installed inside the separation box 6. Multiple sealing mechanisms 9 are installed inside the protective pad 8. Each sealing mechanism 9 includes multiple sealing caps 901, multiple first separation tubes 902, multiple sealing gaskets 903, multiple threaded springs 904, multiple rotating rings 905, multiple fixing rings 906, multiple second separation tubes 907, multiple third separation tubes 908, multiple sealing rings 909, and multiple filter membranes 910. The sealing caps 901 are threaded onto the upper outer surface of each of the first separation tubes 902. The filter membranes 910 are fixedly installed inside the second separation tubes 907 and the third separation tubes 908. The fixing rings 906 are fixedly installed outside the second separation tubes 907 and the third separation tubes 908. Near the upper end of the separation box 6, the lower ends of multiple threaded springs 904 are respectively fixedly mounted on the upper end of multiple fixed rings 906. Multiple rotating rings 905 are respectively threaded on the outside of multiple threaded springs 904 and multiple fixed rings 906. Multiple sealing gaskets 903 are respectively fixedly mounted on opposite sides of multiple threaded springs 904. Multiple sealing rings 909 are respectively fixedly mounted on the upper end of multiple second separation tubes 907 and multiple third separation tubes 908. The lower ends of multiple first separation tubes 902 are respectively threaded on the upper end of multiple second separation tubes 907. The lower ends of multiple second separation tubes 907 are respectively threaded on the upper end of multiple third separation tubes 908. A hinged cover 1 is hinged at the upper end of the separation box 6. A buffer pad 10 is fixedly mounted at the lower end of the hinged cover 1.

[0035] Specifically, during use, first rotate the sealing cap 901 to disconnect it from the first separation tube 902. Then, pour the cell fluid containing cell-like structures into the first separation tube 902. Next, lock the sealing cap 901 back into the first separation tube 902. Since the lower end of the first separation tube 902 is threaded to the upper end of the second separation tube 907, and the lower end of the second separation tube 907 is threaded to the upper end of the third separation tube 908, the sealing ring 909 is compressed to prevent sample leakage during separation. To strengthen the seal between the separation tubes, rotate the rotating ring 905 to move it outside the fixed ring 906 and the threaded spring 904, causing multiple threaded springs 904 to contract inward. This causes the sealing gasket 903 connected to the threaded spring 904 to tightly adhere to the outside of the separation tube, achieving clamping. To enhance the sealing effect between the various separation tubes and make the connection between them more stable, the separation tubes can be placed into the protective pad 8 inside the separation box 6. When the separation box 6 moves up and down, the protective pad 8 and the buffer pad 10 can protect the separation tubes, enhancing the safety and stability of the operation. At the same time, it allows the cells inside the first separation tube 902 to quickly pass through the filter membranes 910 with different pore sizes inside the second separation tube 907 and the third separation tube 908. This results in organoid tissues larger than 50 cubic millimeters remaining inside the first separation tube 902, organoid tissues between 40 and 50 cubic millimeters remaining in the second separation tube 907, and organoid tissues smaller than 40 cubic millimeters remaining in the third separation tube 908. This separates the cells into three different sizes, providing assistance for subsequent scientific research.

[0036] Working Principle: During operation, the sealing cap 901 is rotated to safely unlock it from the first separation tube 902. Then, the pre-prepared cell solution containing cell-like organisms is poured into the first separation tube 902. Next, the sealing cap 901 is tightly threaded onto the first separation tube 902. Since the first, second, and third separation tubes 908 all use precise threaded connections, the sealing ring 909 is compressed during connection, effectively preventing any leakage risk during sample separation. To further enhance the sealing effect between the separation tubes and improve their stability, the rotating ring 905 is rotated, causing it to move threadedly around the fixed ring 906 and the elastic threaded spring 904. When the rotating ring 905 moves outside the threaded spring 904, it causes the threaded spring 904 to contract slightly inward, causing the tightly connected sealing gasket 903 to fit tightly against the outer wall of the separation tube. This achieves multiple clamping and locking of the separation tubes, not only enhancing the sealing effect between the separation tubes but also improving their stability. The sealing performance between the separation tubes is also ensured by the stability of the connection between the separation tubes. Then, the entire assembled separation tube can be placed inside the protective pad 8. When the separation box 6 performs up-and-down reciprocating motion, the protective pad 8 will work in conjunction with the buffer pad 10 under the hinged cover 1 to provide all-round protection for the separation tube. At the same time, as the separation box 6 continues to move, the cells in the first separation tube 902 begin to pass through the filter membranes 910 with different pore sizes preset inside the second separation tube 907 and the third separation tube 908 one by one. This allows organoid tissue larger than 50 cubic millimeters to be trapped in the first separation tube 902, while organoid tissue between 40 and 50 cubic millimeters remains in the second separation tube 907. Finally, organoid tissue smaller than 40 cubic millimeters will smoothly enter and remain in the third separation tube 908, thereby dividing the cells into three different size categories. This not only improves the separation efficiency, but also ensures the safety of operation and the integrity of the sample through multiple seals.

[0037] Secondly, during cell-like separation, the starter motor 307 drives the rotating block 303 at its output end to rotate. The rotation of the rotating block 303 drives the connecting block 302 to rotate, causing it to push the moving frame 2 to slide on the base 4. Since the rotating column 305 rotates inside the moving frame 2 and is externally fixed inside the gear 306, and the gear 306 meshes with the rack 304 on the base 4, when the moving frame 2 moves, the gear 306 will roll along the rack 304, thereby causing the rotating column 305 to move. The rotation of the internal rotation column 305 of the frame 2 will drive the separation box 6 to move up and down inside it, thereby promoting the separation effect of cells in the separation box 6. When the separation box 6 moves up and down, the spring 301 at its lower end will undergo elastic deformation, thereby providing additional vibration energy to the separation box 6, increasing the separation efficiency and effect of cells in the internal separation tube, so that the cells in the first separation tube 902 can be accurately screened according to the filter pore size on the filter membrane 910, improving the accuracy and efficiency of the separation process.

[0038] Finally, it should be noted that the above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Although the present utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A chromatography separation apparatus, comprising a base (4), characterized in that: The base (4) is provided with a separation mechanism (3), which includes multiple springs (301), a connecting block (302), a rotating block (303), a rack (304), a rotating column (305), a gear (306), and a motor (307). A movable frame (2) is slidably provided on the upper end of the base (4), and a separation box (6) is fixedly provided between the upper ends of the multiple springs (301). The separation box (6) is fixedly provided with a protective pad (8), and the protective pad (8) is provided with a plurality of sealing mechanisms (9). The plurality of sealing mechanisms (9) include a plurality of sealing caps (901), a plurality of first separation tubes (902), a plurality of sealing pads (903), a plurality of threaded springs (904), a plurality of rotating rings (905), a plurality of fixed rings (906), a plurality of second separation tubes (907), a plurality of third separation tubes (908), a plurality of sealing rings (909), and a plurality of filter membranes (910).

2. The chromatography separation apparatus according to claim 1, characterized in that: The lower ends of the multiple springs (301) are fixedly disposed inside the lower end of the movable frame (2), the external rotating column (305) is rotatably disposed inside the lower end of the movable frame (2), and the internal gear (306) is fixedly disposed outside the rotating column (305).

3. The chromatography separation apparatus according to claim 1, characterized in that: The lower end of the rack (304) is fixedly mounted on the upper end of the base (4), the gear (306) is externally meshed with the upper end of the rack (304), and the separation box (6) is externally slidably mounted inside the moving frame (2).

4. The chromatography separation apparatus according to claim 1, characterized in that: The motor (307) is externally fixed inside the base (4) on one side. The rotating block (303) is fixed on one side at the output end of the motor (307). The front end of the connecting block (302) is rotatably mounted on one side of the rotating block (303) on the other side near the rear end. The moving frame (2) is rotatably mounted on one side of the connecting block (302).

5. The chromatography separation apparatus according to claim 1, characterized in that: The interior of the plurality of sealing caps (901) is threaded onto the upper part of the exterior of the plurality of first separation tubes (902). The exterior of the plurality of filter membranes (910) is fixedly disposed inside the plurality of second separation tubes (907) and the plurality of third separation tubes (908). The interior of the plurality of fixing rings (906) is fixedly disposed on the upper part of the exterior of the plurality of second separation tubes (907) and the plurality of third separation tubes (908). The lower end of the plurality of threaded spring pieces (904) is fixedly disposed on the upper end of the plurality of fixing rings (906). The interior of the plurality of rotating rings (905) is threaded onto the exterior of the plurality of threaded spring pieces (904) and the plurality of fixing rings (906).

6. The chromatography separation apparatus according to claim 1, characterized in that: Multiple sealing gaskets (903) are fixedly disposed on opposite sides of multiple threaded springs (904). Multiple sealing rings (909) are fixedly disposed on the upper part of multiple second separation tubes (907) and multiple third separation tubes (908). The lower ends of multiple first separation tubes (902) are threaded onto the upper ends of multiple second separation tubes (907). The lower ends of multiple second separation tubes (907) are threaded onto the upper ends of multiple third separation tubes (908).

7. The chromatography separation apparatus according to claim 1, characterized in that: Four anti-slip pads (7) are fixedly installed at the lower end of the base (4), and a control panel (5) is fixedly installed on one side of the front end of the base (4). A hinge cover (1) is hinged to the upper end of the separation box (6), and a buffer pad (10) is fixedly installed at the lower end of the hinge cover (1).

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