Stem cell culture perfusion device

Abstract

The utility model provides a stem cell culture perfusion device, and relates to the technical field of stem cell culture perfusion. Comprising a culture cylinder body, a combined cover and a liquid storage cylinder, a pump body is arranged at the top end of the combined cover, a cleaning ball is rotatably arranged in the combined cover, a first gear is arranged on the outer side of the cleaning ball, and a second gear is arranged at the output end of a second motor. Through operation of a second motor, a cleaning ball can rotate in a culture barrel, the use flexibility of the cleaning ball is improved, when a pump body operates to extract 70% ethyl alcohol or 70% isopropyl alcohol stored in a liquid storage barrel, the 70% ethyl alcohol or 70% isopropyl alcohol after pressurization can be conveyed into the cleaning ball, and the cleaning ball can be used for cleaning the culture barrel. 70% ethanol or 70% isopropanol after pressurization can be sprayed into the culture cylinder through the nozzle on the outer side of the cleaning ball to spray and wash the culture cylinder and the stirring assembly in the culture cylinder after use, so that the cleaning efficiency of the perfusion device is effectively improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to a perfusion device, in particular to a stem cell culture perfusion device, belongs to stem cell culture perfusion technical field. BACKGROUND

[0002] Stem cells are a kind of cells with self-replication ability and multi-potential differentiation ability, according to its source and differentiation potential, can be divided into embryonic stem cells, adult stem cells, totipotent stem cells, pluripotent stem cells and unipotent stem cells, the development of stem cell culture technology provides new possibility for medical research and treatment, including tissue repair, organ regeneration, disease model establishment and drug screening etc. With the continuous progress of technology, stem cell therapy is expected to become an important part of future medicine, and provide new treatment strategies for treating various diseases.

[0003] At present, the existing stem cell culture perfusion device needs to be disinfected and cleaned after use to prevent pollution, for example, 70% ethanol or 70% isopropyl alcohol can be used to wipe the fluid unit for disinfection, and during the cleaning and disinfection perfusion process, relevant laboratory safety procedures need to be followed, such as wearing gloves, goggles, etc., manual cleaning of the perfusion device is not only slow, but also has certain danger, therefore, we provide a stem cell culture perfusion device to solve the above problems. UTILITY MODEL CONTENTS

[0004] To solve the above problems, the utility model provides a stem cell culture perfusion device to solve the above problems, and the specific technical scheme is:

[0005] A stem cell culture perfusion device, comprising a culture cylinder, a combination cover and a liquid storage cylinder, the top of the combination cover is provided with a pump body, a cleaning ball is rotatably arranged in the inside of the combination cover, and the top of the cleaning ball is rotatably connected with the output end of the pump body, a No. 1 gear is arranged on the outside of the cleaning ball, a mounting bracket is arranged at the top of the combination cover, a No. 2 motor is arranged at the top of the mounting bracket, a No. 2 gear is arranged at the output end of the No. 2 motor, and the No. 2 gear is meshed and connected with the No. 1 gear.

[0006] Preferably, the combination cover is arranged at the top of the culture cylinder, and a liquid injection port is arranged through the inside of the top of the combination cover.

[0007] Preferably, a No. 1 motor is arranged at the top of the combination cover, a connecting shaft is arranged at the output end of the No. 1 motor, three groups of stirring rods are arranged on the outside of the connecting shaft, a No. 1 spiral blade is arranged on the outside of the connecting shaft, and the No. 1 spiral blade is below the stirring rods, a No. 2 spiral blade is arranged on the outside of the connecting shaft, and the No. 2 spiral blade is below the No. 1 spiral blade.

[0008] Preferably, the top end of the combined cover is provided with a circulating pump, the input end of the circulating pump is provided with a first infusion tube, and the first infusion tube is in communication with the inside of the culture cylinder body, the output end of the circulating pump is provided with a second infusion tube, and the second infusion tube is in communication with the inside of the combined cover.

[0009] Preferably, the outside of the culture cylinder body is provided with two groups of positioning frames, and the positioning frames are located outside the first infusion tube.

[0010] Preferably, the top end of the combined cover is provided with a support frame, and the support frame is in contact with the bottom end of the liquid storage cylinder, the bottom end of the culture cylinder body is provided with a perfusion port in the inside, and the inside of the liquid storage cylinder is provided with a floating ball assembly.

[0011] Compared with the prior art, the utility model has the following beneficial effects:

[0012] 1、The utility model discloses a perfusion device, which comprises a culture cylinder body, a combined cover, a liquid storage cylinder and a support frame, wherein the culture cylinder body is provided with a stirring assembly, the combined cover is provided with a circulating pump, the liquid storage cylinder is provided with a floating ball assembly, and the support frame is in contact with the bottom end of the liquid storage cylinder.

[0013] 2、The utility model discloses a perfusion device, which comprises a culture cylinder body, a combined cover, a liquid storage cylinder and a support frame, wherein the culture cylinder body is provided with a stirring assembly, the combined cover is provided with a circulating pump, the liquid storage cylinder is provided with a floating ball assembly, and the support frame is in contact with the bottom end of the liquid storage cylinder. BRIEF DESCRIPTION OF DRAWINGS

[0014] Figure 1 It is a whole structure schematic view of the utility model;

[0015] Figure 2 It is a culture cylinder body shearing structure schematic view of the utility model;

[0016] Figure 3 It is a structure schematic view of the utility model's first spiral blade and second spiral blade;

[0017] Figure 4 It is the combined cover bottom end structure schematic view of the utility model;

[0018] Figure 5 It is the liquid storage cylinder structure schematic view of the utility model;

[0019] Figure 6 It is the liquid storage cylinder shearing structure schematic view of the utility model.

[0020] BRIEF DESCRIPTION OF DRAWINGS:1, culture cylinder body;2, combined cover;3, liquid injection port;4, No. 1 motor;5, connecting shaft;6, stirring rod;7, No. 1 spiral blade;8, No. 2 spiral blade;9, circulating pump;10, No. 1 infusion tube;11, No. 2 infusion tube;12, positioning frame;13, perfusion port;14, support frame;15, liquid storage cylinder;16, floating ball assembly;17, pump body;18, cleaning ball;19, No. 1 gear;20, mounting bracket;21, No. 2 motor;22, No. 2 gear. DETAILED DESCRIPTION

[0021] The utility model will be further described with reference to the drawings.

[0022] Please refer to Figure 1 、 Figure 2 、 Figure 3 、 Figure 4 、 Figure 5 And Figure 6 , a stem cell culture perfusion device;

[0023] Including culture cylinder body 1, combined cover 2 and liquid storage cylinder 15, the top of combined cover 2 is provided with pump body 17, the inside rotation of combined cover 2 is provided with cleaning ball 18, and the top of cleaning ball 18 is rotatably connected with the output end of pump body 17, the outside of cleaning ball 18 is provided with No. 1 gear 19, the top of combined cover 2 is provided with mounting bracket 20, the top of mounting bracket 20 is provided with No. 2 motor 21, the output end of No. 2 motor 21 is provided with No. 2 gear 22, and No. 2 gear 22 is meshedly connected with No. 1 gear 19.

[0024] The outer side of the cleaning ball 18 is obliquely provided with a plurality of groups of nozzles. The cleaning ball 18 is rotatably arranged in the interior of the combined cover 2 by means of bearings. The top end of the cleaning ball 18 is rotatably connected with the output end of the pump body 17. The pump body 17 is inserted into the interior of the cleaning ball 18 by means of bearings, so as to avoid liquid leakage. When the pump body 17 operates to extract 70% ethanol or 70% isopropyl alcohol stored in the liquid storage cylinder 15, the 70% ethanol or 70% isopropyl alcohol after being pressurized can be delivered into the cleaning ball 18. The 70% ethanol or 70% isopropyl alcohol after being pressurized can be sprayed into the culture cylinder 1 through the nozzles on the outer side of the cleaning ball 18, so as to spray and wash the culture cylinder 1 and the internal stirring assembly after use. The mounting bracket 20 is used for providing mounting support for the second motor 21. The second gear 22 at the output end of the second motor 21 is in meshing connection with the first gear 19 on the outer side of the cleaning ball 18. The operation of the second motor 21 can make the cleaning ball 18 rotate in the interior of the culture cylinder 1, increase the flexibility of the cleaning ball 18, and effectively improve the cleaning efficiency of the perfusion device.

[0025] Referring to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 , a stem cell culture perfusion device;

[0026] The combined cover 2 is arranged at the top end of the culture cylinder 1. The top end of the combined cover 2 is provided with a liquid injection opening 3 arranged through the interior. The top end of the combined cover 2 is provided with a support bracket 14, and the support bracket 14 is in contact with the bottom end of the liquid storage cylinder 15. The bottom end of the culture cylinder 1 is provided with a perfusion opening 13 arranged through the interior. The interior of the liquid storage cylinder 15 is provided with a floating ball assembly 16.

[0027] The culture cylinder 1 is a main combined component of the stem cell culture perfusion device. The combined cover 2 can cover the top end of the culture cylinder 1. The stem cell tissue liquid can be put into the interior of the culture cylinder 1 through the liquid injection opening 3. The support bracket 14 is used for providing a suitable mounting height for the liquid storage cylinder 15. The floating ball assembly 16 is composed of a floating ball and a scale. The floating ball assembly 16 can be used for facilitating the staff to check the remaining amount of 70% ethanol or 70% isopropyl alcohol in the interior of the liquid storage cylinder 15 in real time. The interiors of the liquid injection opening 3 and the perfusion opening 13 are both provided with electromagnetic valves. The stem cell tissue liquid in the interior of the culture cylinder 1 can be discharged through the perfusion opening 13.

[0028] Referring to Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6 , a stem cell culture perfusion device;

[0029] The top end of the combined cover 2 is provided with a first motor 4, the output end of the first motor 4 is provided with a connecting shaft 5, the outer side of the connecting shaft 5 is provided with three groups of stirring rods 6, the outer side of the connecting shaft 5 is provided with a first spiral blade 7, and the first spiral blade 7 is located below the stirring rod 6, the outer side of the connecting shaft 5 is provided with a second spiral blade 8, and the second spiral blade 8 is located below the first spiral blade 7, the top end of the combined cover 2 is provided with a circulating pump 9, the input end of the circulating pump 9 is provided with a first infusion tube 10, and the first infusion tube 10 is through with the inside of the culture cylinder 1, the output end of the circulating pump 9 is provided with a second infusion tube 11, and the second infusion tube 11 is through with the inside of the combined cover 2, the outer side of the culture cylinder 1 is provided with two groups of positioning frames 12, and the positioning frame 12 is located outside the first infusion tube 10.

[0030] The connecting shaft 5 is connected with the output end of the first motor 4, the three groups of stirring rods 6, the first spiral blade 7 and the second spiral blade 8 are installed on the outer side of the connecting shaft 5 in stages, when the first motor 4 operates, the connecting shaft 5 can drive the three groups of stirring rods 6, the first spiral blade 7 and the second spiral blade 8 to rotate in the inside of the culture cylinder 1, the first spiral blade 7 and the second spiral blade 8 can push the stem cell tissue liquid under the culture cylinder 1 to the top of the culture cylinder 1, the stem cell tissue liquid under the culture cylinder 1 can flow to the top of the culture cylinder 1 through the first infusion tube 10 and the second infusion tube 11 by the operation of the circulating pump 9, so that the activity of the stem cells is improved more quickly, the stirring and mixing effect is better, and the stem cell tissue liquid can be pushed to the perfusion port 13 by the first spiral blade 7 and the second spiral blade 8 in reverse rotation, so that the liquid output of the perfusion port 13 is effectively improved.

[0031] The electrical equipment in the application is a common electrical equipment in the prior art, and the model or internal structure thereof will not be described in detail, and it can also be replaced by other power sources.

[0032] The technical principle of the utility model is described above in combination with specific embodiments. These descriptions are only for explaining the principle of the utility model, and cannot be interpreted as limiting the protection scope of the utility model in any way. Based on the explanation herein, other specific embodiments of the utility model can be conceived by those skilled in the art without creative labor, and these embodiments will fall within the protection scope of the claims of the utility model.

Claims

1. A stem cell culture perfusion device comprising a culture cartridge (1), a combination cover (2) and a liquid storage cartridge (15), characterized in that: The top end of the combined cover (2) is provided with a pump body (17), the inside of the combined cover (2) is rotatably provided with a cleaning ball (18), and the top end of the cleaning ball (18) is rotatably connected with the output end of the pump body (17), the outside of the cleaning ball (18) is provided with a first gear (19), the top end of the combined cover (2) is provided with a mounting frame (20), the top end of the mounting frame (20) is provided with a second motor (21), the output end of the second motor (21) is provided with a second gear (22), and the second gear (22) is meshedly connected with the first gear (19).

2. The stem cell culture perfusion device of claim 1, wherein: The combined cover (2) is arranged at the top end of the culture cylinder (1), and the top end of the combined cover (2) is provided with a liquid injection port (3) penetratingly arranged inside.

3. The stem cell culture perfusion device of claim 1, wherein: The top end of the combined cover (2) is provided with a first motor (4), the output end of the first motor (4) is provided with a connecting shaft (5), the outside of the connecting shaft (5) is provided with three groups of stirring rods (6), the outside of the connecting shaft (5) is provided with a first spiral blade (7), and the first spiral blade (7) is located below the stirring rod (6), the outside of the connecting shaft (5) is provided with a second spiral blade (8), and the second spiral blade (8) is located below the first spiral blade (7).

4. The stem cell culture perfusion device of claim 1, wherein: The top end of the combined cover (2) is provided with a circulating pump (9), the input end of the circulating pump (9) is provided with a first infusion tube (10), and the first infusion tube (10) penetrates the inside of the culture cylinder (1), the output end of the circulating pump (9) is provided with a second infusion tube (11), and the second infusion tube (11) penetrates the inside of the combined cover (2).

5. The stem cell culture perfusion device of claim 1, wherein: The outside of the culture cylinder (1) is provided with two groups of positioning frames (12), and the positioning frame (12) is located outside the first infusion tube (10).

6. The stem cell culture perfusion device of claim 1, wherein: The top end of the combined cover (2) is provided with a supporting frame (14), and the supporting frame (14) is in contact with the bottom end of the liquid storage cylinder (15), the bottom end of the culture cylinder (1) is provided with a perfusion port (13) penetratingly arranged inside, and the inside of the liquid storage cylinder (15) is provided with a floating ball assembly (16).

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