Novel PCR plate for magnetic bead purification

Abstract

The utility model relates to the technical field of molecular biology experiments, in particular to a novel PCR plate for magnetic bead purification, which comprises a plate body, a first auxiliary module and a second auxiliary module. A plurality of holes are formed in the top of the plate body, and diversion structures are arranged at the bottoms of the holes to optimize magnetic bead distribution; a uniform mixing mechanism is arranged in the first auxiliary module and can be used for automatically and uniformly mixing liquid and adsorbing impurities; and a transfer mechanism is arranged in the second auxiliary module, so that liquid can be accurately transferred. The distribution uniformity of the magnetic beads is improved through the flow guide structure, the influence of residues is reduced by means of uniform mixing and cleaning functions, and liquid treatment and transfer can still be completed when the auxiliary module breaks down. The magnetic bead purification efficiency and the experimental result consistency can be remarkably improved, and meanwhile, the equipment operation reliability is enhanced.

Description

Technical Field

[0001] This invention belongs to the field of biological experiment and molecular diagnostic technology, specifically a novel PCR plate for magnetic bead purification. Background Technology

[0002] In molecular biology experiments, PCR (polymerase chain reaction) technology is widely used for gene amplification, gene detection, and nucleic acid extraction. To improve experimental efficiency and result reliability, PCR products usually need to be purified to remove impurities or unreacted components from the reaction system. Magnetic bead purification, as an efficient and convenient method for nucleic acid purification, has been widely used in recent years. However, in the process of purification using magnetic beads, the design and performance of the PCR plate have a significant impact on the convenience of experimental operation and the purification effect.

[0003] Existing PCR plates have certain limitations in magnetic bead purification. For example, the bottom design of traditional PCR plates is usually flat or round. When used with a magnetic rack, this structure may lead to uneven aggregation of magnetic beads, thus affecting purification efficiency. Furthermore, the materials and surface treatment processes of existing PCR plates may not fully meet the requirements for magnetic bead adsorption and elution, easily resulting in magnetic bead residue or sample loss. Simultaneously, in multi-step purification operations, the accuracy of liquid transfer and mixing is also affected by the PCR plate design, which may reduce the consistency and reproducibility of experimental results.

[0004] Although several improved PCR plates are available on the market, these products still have some shortcomings in practical applications. For example, while some PCR plates have optimized well bottom shapes, uneven distribution of magnetic beads may still occur when used with a magnetic rack; other PCR plates, due to inappropriate material selection, may deform or become contaminated under high temperatures or chemical reagent environments, affecting the accuracy of experiments. Therefore, designing a new type of PCR plate that is better suited for magnetic bead purification operations has become an urgent technical problem to be solved. Utility Model Content

[0005] This invention relates to the field of molecular biology experimental technology, specifically to a novel PCR plate for magnetic bead purification. Existing PCR plates have several shortcomings in the magnetic bead purification process, such as unreasonable well bottom design leading to uneven bead aggregation, inadequate material and surface treatment processes failing to meet adsorption and elution requirements, and limited precision in liquid transfer and mixing operations. These problems not only affect the efficiency of magnetic bead purification but may also lead to sample loss or decreased consistency of experimental results.

[0006] To address the aforementioned issues, this invention proposes a novel PCR plate for magnetic bead purification, comprising a plate body. The top of the plate body has multiple wells for containing reaction solution, and each well has a flow guiding structure at its bottom. A first auxiliary module is mounted on the outer wall of the plate body, and a mixing mechanism is provided inside the first auxiliary module. A second auxiliary module is slidably mounted on one side of the outer wall of the plate body, and a transfer mechanism is provided inside the second auxiliary module.

[0007] Preferably, the flow guiding structure includes multiple first grooves and a second groove at the bottom of the orifice. First flow guiding vanes are installed inside each of the multiple first grooves. A flow dividing plate is installed on the top of two cooperating first flow guiding vanes. A third groove is formed on the outer wall of one of the two flow dividing plates that are close to each other. A third flow guiding vane is installed inside each of the two third grooves. An adjusting block is rotatably installed at the end of each of the two third flow guiding vanes away from the third groove. An adjusting plate is rotatably installed at the end of each of the two adjusting blocks away from the third flow guiding vane. A flexible coating is installed on the outer walls of both sides of the two adjusting plates. A second flow guiding vane is installed inside the second groove. A cylindrical groove is formed inside the second flow guiding vane. A telescopic limiting rod is installed inside the cylindrical groove. A limiting ring is rotatably installed at the end of the orifice away from the second flow guiding vane.

[0008] Preferably, the mixing mechanism includes a first cavity opened inside the first auxiliary module. The inner walls on both sides of the first cavity are provided with fourth grooves. A fourth guide vane is installed inside each of the two fourth grooves. A first mounting base is installed at the end of the two fourth guide vanes away from the fourth groove. A first driving device is installed inside the first mounting base. A first mixing plate is installed at the output end of the first driving device. Multiple stirring blades are installed at the bottom edge of the first mixing plate. Multiple first adsorption components are installed at the center of the bottom of the first mixing plate. A waste liquid collection box is slidably inserted inside the first auxiliary module.

[0009] Preferably, a positioning groove is provided on one side of the outer wall of the plate, and a threaded rod is rotatably installed inside the positioning groove. The bottom end of the second auxiliary module is threadedly engaged with the outer wall of the threaded rod.

[0010] Preferably, the transfer mechanism includes a second cavity opened inside the second auxiliary module. The inner walls on both sides of the second cavity are provided with fifth grooves. A fifth guide vane is installed inside each of the two fifth grooves. A second mounting seat is installed at the end of the two fifth guide vanes away from the fifth groove. A second driving device is installed inside the second mounting seat. A second mixing plate is installed at the output end of the second driving device. A plurality of second adsorption components are installed at the bottom of the second mixing plate.

[0011] Preferably, a roller with a transfer film wound on its surface is rotatably mounted on one side of the outer wall of the second auxiliary module, two drive rollers are rotatably mounted inside the second auxiliary module, and a fixed bracket is mounted inside the second auxiliary module near the two drive rollers. The end of the transfer film away from the roller passes through the gap between the second auxiliary module and the two drive rollers, and the end of the transfer film away from the roller is placed on top of the fixed bracket.

[0012] Preferably, both the first auxiliary module and the second auxiliary module are connected to the plate body by a sliding installation method, and both the first auxiliary module and the second auxiliary module can move along the outer wall of the plate body to adjust the working position.

[0013] Preferably, the limiting ring can be rotated to a horizontal state, and is used to achieve emergency liquid transportation and treatment through the cooperation of the diversion plate, adjustment plate and flexible coating at the bottom of multiple holes when the first auxiliary module or the second auxiliary module fails.

[0014] The beneficial effects of this utility model are:

[0015] Before the magnetic bead purification operation, it can improve the purification efficiency and the consistency of experimental results. After adding the reaction solution to the well, it can avoid the influence of impurities on subsequent purification operations. After mixing the liquid in the well, if residue is found on the inner wall of the well, multiple components such as one of the adjustment plates can be controlled to reduce the adverse effects of residue on experimental results. If the plate is used, the normal operation of the plate will not be affected by the failure of the auxiliary module. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of this utility model.

[0017] Figure 2 This is a partially enlarged schematic diagram of the flow guiding structure in this utility model.

[0018] Figure 3 This is a cross-sectional view of the mixing mechanism in this utility model.

[0019] Figure 4 This is a cross-sectional view of the transfer mechanism in this utility model.

[0020] Figure 5 This is a schematic diagram showing the connection relationship between the second auxiliary module and the threaded rod in this utility model.

[0021] Figure 6 This is a schematic diagram of the emergency operation procedure for the auxiliary module failure of this utility model.

[0022] The attached figures are labeled as follows:

[0023] 1. Plate body; 2. Hole position; 3. First auxiliary module; 4. Second auxiliary module; 5. First groove; 6. Second groove; 7. First guide vane; 8. Diverter plate; 9. Third groove; 10. Third guide vane; 11. Adjusting block; 12. Adjusting plate; 13. Flexible coating; 14. Second guide vane; 15. Cylindrical groove; 16. Telescopic limit rod; 17. Limiting ring; 18. First cavity; 19. Fourth groove; 20. Fourth guide vane; 21. 1. Mounting base; 22. First driving device; 23. First mixing plate; 24. Stirring blade; 25. First adsorption assembly; 26. Waste liquid collection box; 27. Positioning groove; 28. Threaded rod; 29. ​​Second cavity; 30. Fifth groove; 31. Fifth guide vane; 32. Second mounting base; 33. Second driving device; 34. Second mixing plate; 35. Second adsorption assembly; 36. Roller; 37. Transfer membrane; 38. Drive roller; 39. Fixed bracket. Detailed Implementation

[0024] This utility model relates to a novel PCR plate for magnetic bead purification. Its structural design and functional implementation are both focused on improving magnetic bead purification efficiency, optimizing experimental procedures, and enhancing the consistency of experimental results. The following description, in conjunction with the appendix... Figure 1 To be continued Figure 6 The specific embodiments of this utility model will be described in detail.

[0025] like Figure 1 As shown, the PCR plate of this invention includes a plate body 1, with multiple wells 2 on the top of the plate body 1 for containing reaction solution. Each well 2 has a flow guiding structure at its bottom, which consists of components such as a first groove 5, a second groove 6, a first flow guide 7, a flow splitter 8, a third flow guide 10, an adjusting block 11, an adjusting plate 12, and a flexible coating 13. Figure 2 As shown. A first guide vane 7 is installed in the first groove 5. A diverter plate 8 is installed on the top of two cooperating first guide vanes 7. A third groove 9 is formed on the outer wall of the diverter plate 8 on the side closest to the diverter plate 8. A third guide vane 10 is installed inside the third groove 9. An adjusting block 11 is rotatably installed on the end of the third guide vane 10 away from the third groove 9. An adjusting plate 12 is rotatably installed on the end of the adjusting block 11 away from the third guide vane 10. Flexible coatings 13 are installed on both outer walls of the adjusting plate 12. In addition, a second guide vane 14 is installed in the second groove 6. A cylindrical groove 15 is formed inside the second guide vane 14. A telescopic limiting rod 16 is installed inside the cylindrical groove 15. A limiting ring 17 is rotatably installed on the end of the hole 2 away from the second guide vane 14. The above-mentioned guide structure design can effectively optimize the distribution of magnetic beads in the hole 2, ensuring that the magnetic bead adsorption and elution process is more uniform and efficient.

[0026] In practical applications, after the reaction solution is added to orifice 2, the magnetic beads will settle to the bottom of orifice 2 due to gravity. At this time, the first guide vane 7 and the flow divider 8, through their specific geometric shapes and positional relationships, guide the magnetic beads to gather towards the central area of ​​orifice 2, preventing the magnetic beads from accumulating at the bottom edge of the orifice. Simultaneously, the cooperation of the third guide vane 10 with the adjusting block 11 and adjusting plate 12 further enhances the uniformity of the magnetic bead distribution. The flexible coating 13 on the adjusting plate 12 can contact the inner wall of orifice 2 when needed, cleaning residues adhering to the orifice wall, thereby reducing the impact of impurities on experimental results. Furthermore, the combined design of the second guide vane 14 and the telescopic limiting rod 16 can dynamically adjust the spatial structure at the bottom of orifice 2 to accommodate different volumes of reaction solution.

[0027] To further improve the liquid mixing effect, this utility model has a first auxiliary module 3 installed on the outer wall of the plate 1, which has a mixing mechanism inside. Figure 3 As shown, the mixing mechanism includes a first cavity 18. Fourth grooves 19 are formed on the inner walls of both sides of the first cavity 18. Fourth guide vanes 20 are installed inside the fourth grooves 19. A first mounting base 21 is installed at the end of each of the two fourth guide vanes 20 furthest from the fourth grooves 19. A first driving device 22 is installed inside the first mounting base 21. A first mixing plate 23 is installed at the output end of the first driving device 22. Multiple stirring blades 24 are installed at the bottom edge of the first mixing plate 23, and multiple first adsorption components 25 are installed at the center of the bottom. In addition, a waste liquid collection box 26 is slidably inserted inside the first auxiliary module 3. In actual operation, when the reaction liquid is added to the orifice 2, the first driving device 22 is activated, driving the first mixing plate 23 to rotate. The stirring blades 24 then thoroughly mix the liquid in the orifice 2. If insufficient mixing or impurities are found, the first adsorption components 25 can automatically adsorb the impurities in the liquid and transfer them to the waste liquid collection box 26, thereby ensuring the smooth progress of subsequent purification operations.

[0028] To further achieve precise liquid transfer, this invention features a second auxiliary module 4 slidably mounted on the outer wall of one side of the plate 1, which contains a transfer mechanism. For example... Figure 4As shown, the transfer mechanism includes a second cavity 29. Fifth grooves 30 are formed on the inner walls of both sides of the second cavity 29. Fifth guide vanes 31 are installed inside the fifth grooves 30. A second mounting base 32 is mounted on the ends of the two fifth guide vanes 31 furthest from the fifth grooves 30. A second driving device 33 is installed inside the second mounting base 32. A second mixing plate 34 is installed at the output end of the second driving device 33. Multiple second adsorption components 35 are installed at the bottom of the second mixing plate 34. Furthermore, a roller 36 with a transfer film 37 wound onto its surface is rotatably mounted on one side of the outer wall of the second auxiliary module 4. Two drive rollers 38 are rotatably mounted inside the second auxiliary module 4. A fixed bracket 39 is mounted inside the second auxiliary module 4 near the two drive rollers 38. The end of the transfer film 37 furthest from the roller 36 passes through the gap between the second auxiliary module 4 and the two drive rollers 38 and is placed on top of the fixed bracket 39. In actual operation, when liquid needs to be transferred from one orifice 2 to another, the second drive device 33 is activated, driving the second mixing plate 34 to rotate. The second adsorption component 35 adsorbs the liquid and transfers it to the target orifice 2. The transfer membrane 37 is used to assist in the precise transfer of liquid, ensuring that the liquid does not leak or become contaminated.

[0029] like Figure 5 As shown, the bottom end of the second auxiliary module 4 is threaded into the outer wall of the threaded rod 28, which is installed inside the positioning groove 27. This sliding installation method allows the second auxiliary module 4 to move along the outer wall of the plate 1 according to actual needs, thereby flexibly adjusting the working position of the transfer mechanism. Furthermore, as... Figure 6 As shown, when the first auxiliary module 3 or the second auxiliary module 4 malfunctions, the limiting rings 17 at the top of the multiple holes 2 can be controlled to rotate downwards to a horizontal position. The loading robot then adds the reaction liquid to the rightmost hole 2. With the cooperation of components such as the diversion plate 8, adjusting plate 12, and flexible coating 13 at the bottom of the multiple holes 2, the liquid is transported to the top of the hole 2 located below the transfer mechanism. Subsequently, the adjusting plate 12 and flexible coating 13 inside the hole 2 sequentially mix and clean the liquid, while the transfer mechanism continues to precisely transfer the liquid. After the liquid has been transferred, the adjusting plate 12 inside the hole 2 continues to push it to the left, causing the liquid to fall onto the top of the leftmost hole 2, where the unloading robot automatically picks it up and unloads it. This emergency operation process ensures that even if the auxiliary module malfunctions, the plate 1 can still operate normally, thereby improving the reliability and stability of the equipment.

[0030] In summary, this invention significantly improves the efficiency of magnetic bead purification and the consistency of experimental results through the synergistic design of the flow guiding structure, mixing mechanism, transfer mechanism, and emergency operation procedure, reducing sample loss and impurity interference, and providing a more efficient and reliable tool for molecular biology experiments.

[0031] To enable those skilled in the art to fully understand and implement this utility model, the following provides further supplementary explanations of the specific implementation principles and operating steps of this utility model.

[0032] First, in the initial stage of the magnetic bead purification operation, the reaction solution needs to be added to well 2 on plate 1. For example... Figure 1 As shown, each hole 2 has a flow guiding structure at its bottom, the specific structure of which can be found in the reference. Figure 2 After the reaction solution is injected, the magnetic beads settle to the bottom of orifice 2 due to gravity. At this time, the first guide vane 7 and the flow divider 8 in the first groove 5 work together to guide the magnetic beads to gather in the central area of ​​orifice 2 through their specific geometric shape and positional relationship, avoiding the accumulation of magnetic beads at the bottom edge of the orifice. At the same time, the cooperation of the third guide vane 10 with the adjusting block 11 and the adjusting plate 12 further optimizes the uniformity of the distribution of magnetic beads. The flexible coating 13 installed on the outer walls of both sides of the adjusting plate 12 can contact the inner wall of orifice 2 when needed to clean the residues attached to the orifice wall, thereby reducing the impact of impurities on the experimental results. In addition, the combined design of the second guide vane 14 and the telescopic limiting rod 16 can dynamically adjust the spatial structure at the bottom of orifice 2 according to actual needs to adapt to the needs of different volumes of reaction solution.

[0033] Secondly, during the liquid mixing stage, the mixing mechanism in the first auxiliary module 3 is activated. For example... Figure 3 As shown, the mixing mechanism includes a first cavity 18, with fourth grooves 19 formed on the inner walls of both sides of the first cavity 18. Fourth guide vanes 20 are installed inside the fourth grooves 19. A first mounting base 21 is mounted on the end of each of the two fourth guide vanes 20 furthest from the fourth groove 19. A first driving device 22 is installed inside the first mounting base 21. When the first driving device 22 is activated, its output end drives the first mixing plate 23 to rotate. Multiple stirring blades 24 mounted on the bottom edge of the first mixing plate 23 then thoroughly mix the liquid in the orifice 2. If insufficient mixing or impurities are found, multiple first adsorption components 25 mounted at the center of the bottom of the first mixing plate 23 automatically adsorb the impurities in the liquid and transfer them to the waste liquid collection box 26, thereby ensuring the smooth progress of subsequent purification operations.

[0034] Subsequently, during the liquid transfer phase, the transfer mechanism in the second auxiliary module 4 is activated. For example... Figure 4As shown, the transfer mechanism includes a second cavity 29, with fifth grooves 30 formed on the inner walls of both sides of the second cavity 29. Fifth guide vanes 31 are installed inside the fifth grooves 30. A second mounting base 32 is mounted on the end of each of the two fifth guide vanes 31 furthest from the fifth grooves 30. A second driving device 33 is installed inside the second mounting base 32. When the second driving device 33 is activated, its output end drives the second mixing plate 34 to rotate. Multiple second adsorption components 35 mounted at the bottom of the second mixing plate 34 adsorb the liquid and transfer it to the target orifice 2. During this process, a roller 36 with a transfer film 37 wound on its surface and two drive rollers 38 work together to ensure that the liquid is accurately transferred to the target orifice 2, preventing leakage or contamination.

[0035] In addition, such as Figure 5 As shown, the bottom end of the second auxiliary module 4 is threaded into the outer wall of the threaded rod 28, which is installed inside the positioning groove 27. This sliding installation method allows the second auxiliary module 4 to move along the outer wall of the plate 1 according to actual needs, thereby flexibly adjusting the working position of the transfer mechanism. In the event of a malfunction in the first auxiliary module 3 or the second auxiliary module 4, the experimental operation can continue through the emergency operation procedure. Figure 6 As shown, the limiting ring 17 at the top of the multiple orifices 2 rotates downwards to a horizontal position. After the loading robot adds the reaction liquid to the rightmost orifice 2, the liquid is transported to the top of the orifice 2 below the transfer mechanism through the cooperation of components such as the diversion plate 8, adjusting plate 12, and flexible coating 13 at the bottom of the multiple orifices 2. Subsequently, the adjusting plate 12 and flexible coating 13 inside the orifice 2 mix and clean the liquid in sequence, while the transfer mechanism continues to precisely transfer the liquid. After the liquid has been transferred, the adjusting plate 12 inside the orifice 2 continues to push it to the left, causing the liquid to fall onto the top of the leftmost orifice 2, where the unloading robot automatically picks up and unloads the liquid.

[0036] In summary, this invention, through the synergistic design of the flow guiding structure, mixing mechanism, transfer mechanism, and emergency operation procedure, significantly improves the efficiency of magnetic bead purification and the consistency of experimental results, reduces sample loss and impurity interference, and provides a more efficient and reliable tool for molecular biology experiments. All content not described in detail in this specification is prior art known to those skilled in the art, and the model parameters of each electrical component are not specifically limited; conventional equipment can be used. Electrical control components not mentioned in this technical solution are prior art and are therefore not shown in the figures and will not be described further here.

[0037] The above description is only a preferred embodiment of the present utility model and is not intended to limit the present utility model. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present utility model should be included within the protection scope of the present utility model.

Claims

1. A novel PCR plate for purifying magnetic beads, comprising a plate body (1), characterized in that, The top of the plate (1) is provided with a plurality of holes (2) for containing the reaction liquid. Each hole (2) is provided with a flow guiding structure at its bottom. The outer wall of the plate (1) is equipped with a first auxiliary module (3). The first auxiliary module (3) is provided with a mixing mechanism inside. The outer wall of one side of the plate (1) is slidably installed with a second auxiliary module (4). The second auxiliary module (4) is provided with a transfer mechanism inside.

2. The novel PCR plate for magnetic bead purification according to claim 1, characterized in that, The flow guiding structure includes multiple first grooves (5) and one second groove (6) opened at the bottom of the hole (2). First flow guide vanes (7) are installed inside each of the multiple first grooves (5). A flow divider plate (8) is installed on the top of two mating first flow guide vanes (7). A third groove (9) is opened on the outer wall of the two flow dividers (8) on their adjacent sides. A third flow guide vane (10) is installed inside each of the two third grooves (9). A rotatable end of each third flow guide vane (10) is mounted away from the third groove (9). Adjustment blocks (11), both adjustment blocks (11) are rotatably mounted with adjustment plates (12) at the ends away from the third guide plate (10), both sides of the outer walls of the two adjustment plates (12) are covered with flexible coatings (13), the second guide plate (14) is installed inside the second groove (6), the second guide plate (14) has a cylindrical groove (15) inside, the cylindrical groove (15) is installed with a telescopic limit rod (16), and the hole (2) is rotatably mounted with a limit ring (17) at the end away from the second guide plate (14).

3. The novel PCR plate for magnetic bead purification according to claim 1, characterized in that, The mixing mechanism includes a first cavity (18) opened inside the first auxiliary module (3). The inner walls on both sides of the first cavity (18) are provided with fourth grooves (19). The interior of the two fourth grooves (19) is equipped with fourth guide vanes (20). The ends of the two fourth guide vanes (20) away from the fourth grooves (19) are jointly equipped with a first mounting base (21). The interior of the first mounting base (21) is equipped with a first driving device (22). The output end of the first driving device (22) is equipped with a first mixing plate (23). Multiple stirring blades (24) are installed at the bottom edge of the first mixing plate (23). Multiple first adsorption components (25) are installed at the center of the bottom of the first mixing plate (23). A waste liquid collection box (26) is slidably inserted inside the first auxiliary module (3).

4. The novel PCR plate for magnetic bead purification according to claim 1, characterized in that, A positioning groove (27) is provided on one side of the outer wall of the plate (1). A threaded rod (28) is rotatably installed inside the positioning groove (27). The bottom end of the second auxiliary module (4) is threadedly engaged with the outer wall of the threaded rod (28).

5. The novel PCR plate for magnetic bead purification according to claim 1, characterized in that, The transfer mechanism includes a second cavity (29) opened inside the second auxiliary module (4). The inner walls on both sides of the second cavity (29) are provided with fifth grooves (30). The interior of the two fifth grooves (30) is equipped with fifth guide vanes (31). The ends of the two fifth guide vanes (31) away from the fifth grooves (30) are jointly equipped with a second mounting base (32). The interior of the second mounting base (32) is equipped with a second driving device (33). The output end of the second driving device (33) is equipped with a second mixing plate (34). The bottom of the second mixing plate (34) is equipped with multiple second adsorption components (35).

6. The novel PCR plate for magnetic bead purification according to claim 5, characterized in that, A roller (36) with a transfer film (37) wound on its surface is rotatably mounted on one side of the outer wall of the second auxiliary module (4). Two drive rollers (38) are rotatably mounted inside the second auxiliary module (4). A fixed bracket (39) is mounted inside the second auxiliary module (4) near the two drive rollers (38). The end of the transfer film (37) away from the roller (36) passes through the gap between the second auxiliary module (4) and the two drive rollers (38), and the end of the transfer film (37) away from the roller (36) is placed on the top of the fixed bracket (39).

7. The novel PCR plate for magnetic bead purification according to claim 1, characterized in that, The first auxiliary module (3) and the second auxiliary module (4) are both connected to the plate (1) by sliding installation, and the first auxiliary module (3) and the second auxiliary module (4) can move along the outer wall of the plate (1) to adjust the working position.

8. The novel PCR plate for magnetic bead purification according to claim 2, characterized in that, The limiting ring (17) can be rotated to a horizontal state, and is used to realize emergency liquid transportation and treatment through the cooperation of the diversion plate (8), the adjustment plate (12) and the flexible coating (13) at the bottom of the multiple holes (2) when the first auxiliary module (3) or the second auxiliary module (4) fails.

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