Exosome culture device

Abstract

The utility model belongs to the technical field of exosome culture devices, and particularly relates to an exosome culture device. Comprising a device shell, a protective cover is hinged to the device shell, and an exosome shaking and fixing assembly is arranged in the device shell; the exosome shaking and fixing assembly comprises a partition plate, a strip-shaped through hole, a rocker, an arc-shaped plate, a sliding groove, a side plate, a motor, an L-shaped rod, a connecting shaft, a support and a plurality of vessel fixing frames. Through structural improvement and cooperation of the motor, the L-shaped rod and the rocker, shaking of vessels is achieved, the body burden of researchers is relieved, a plurality of vessels can be shaken at the same time, and the overall working efficiency is also improved.

Description

Technical Field

[0001] This utility model belongs to the technical field of exosome culture devices, and specifically relates to an exosome culture device. Background Technology

[0002] Exosomes are tiny vesicles secreted by cells, with a diameter between 40 and 100 nm. They mainly originate from multivesicles formed by the invagination of lysosomal microparticles within the cell, and are released into the extracellular matrix after the outer membrane of the multivesicle fuses with the cell membrane.

[0003] Different cell types have varying abilities to secrete exosomes, and the characteristics of exosomes differ accordingly. Appropriate cells should be selected based on research objectives and needs. For example, mesenchymal stem cells, tumor cells, and immune cells are common cell sources for exosomes. Exosomes derived from mesenchymal stem cells have functions such as immune regulation and tissue repair; exosomes secreted by tumor cells may be closely related to tumor occurrence, development, and metastasis. Generally, it is not necessary to deliberately shake the culture dish during exosome culture; however, some suspension cells or cells with special culture environment requirements may require gentle shaking to promote uniform cell distribution, adequate nutrient uptake, and gas exchange. For example, in the suspension culture of certain tumor cells, moderate shaking can allow cells to better contact the nutrients and oxygen in the culture medium, which is beneficial for cell growth and exosome secretion.

[0004] However, these exosomes that require shaking need to be manually shaken, which can cause researchers to experience significant arm soreness and discomfort. Therefore, a machine is needed to perform regular shaking instead of manual shaking. To address the above problem, an exosome culture device has been proposed. Utility Model Content

[0005] To address the problems existing in the background technology, this utility model provides an exosome culture device; it has the ability to shake and clamp the vessel, reducing the physical burden on researchers.

[0006] This utility model provides an exosome culture device, including a device shell with a protective cover hinged to it. An exosome shaking and fixing assembly is provided inside the device shell. The exosome shaking and fixing assembly includes a partition located inside the device shell, a strip-shaped through hole on the partition, a rocker arm hinged to the through hole, an arc-shaped plate fixedly connected to the rocker arm, a sliding groove within the arc-shaped plate, a side plate fixedly installed on one side of the device shell, a motor fixedly installed on the side plate near the partition, an L-shaped rod fixedly installed at the output end of the motor extending into the device shell, a connecting shaft rotatably connected to the L-shaped rod, the connecting shaft slidingly connected to the sliding groove, and a bracket fixedly installed at one end of the rocker arm extending into the device shell, with several vessel fixing frames evenly arranged on the bracket.

[0007] Furthermore, the vessel fixing frame includes a connecting rod fixedly connected to the support, an arc-shaped frame fixedly connected to the connecting rod, a plurality of connecting rods evenly fixedly connected to the lower side of the arc-shaped frame, a base plate fixedly installed between the connecting rods, a plurality of grooves evenly provided inside the arc-shaped frame, a spring fixedly installed inside the groove, and a telescopic rod fixedly installed at the other end of the spring.

[0008] Furthermore, ball bearings are rotatably connected to the telescopic rod.

[0009] Furthermore, the telescopic rod is slidably connected to the groove.

[0010] Furthermore, the base plate is made of rubber.

[0011] Furthermore, the rocker arm is slidably connected to the strip-shaped through hole.

[0012] Furthermore, a reinforcing ring is fixedly installed between the L-shaped rod and the output end of the motor.

[0013] The beneficial effects of this utility model are:

[0014] This invention, through structural improvements, utilizes a combination of a motor, an L-shaped rod, and a rocker arm to achieve the shaking of vessels, reducing the physical burden on researchers, enabling the shaking of multiple vessels simultaneously, and improving overall work efficiency. Attached Figure Description

[0015] Figure 1 This is a front perspective view of an exosome culture device according to the present invention.

[0016] Figure 2 This is a rear perspective view of an exosome culture device according to the present invention.

[0017] Figure 3 This is a schematic diagram of the exosome culture device of this utility model after the side plate has been removed.

[0018] Figure 4 This is a schematic diagram of the exosome shaking and fixing component of an exosome culture device according to the present invention.

[0019] Figure 5 This is a partial cross-sectional view of the vessel fixing frame of an exosome culture device according to this utility model.

[0020] As shown in the figure:

[0021] 1. Device housing; 2. Protective cover; 3. Partition plate; 4. Strip-shaped through hole; 5. Rocker arm; 6. Arc-shaped plate; 7. Slide groove; 8. Side plate; 9. Motor; 10. L-shaped rod; 11. Connecting shaft; 12. Bracket; 13. Reinforcing ring; 14. Container fixing frame; 1401. Connecting rod; 1402. Arc-shaped frame; 1403. Connecting rod; 1404. Base plate; 1405. Groove; 1406. Spring; 1407. Telescopic rod; 1408. Ball bearing. Detailed Implementation

[0022] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0023] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0024] Please refer to the accompanying diagrams for all instruction manuals:

[0025] An exosome culture device includes a device housing 1, a protective cover 2 hinged to the device housing 1, and an exosome shaking and fixing component inside the device housing 1.

[0026] The exosome shaking and fixing assembly includes a partition 3 located inside the device housing 1. The partition 3 has a strip-shaped through hole 4. A rocker arm 5 is hinged to the strip-shaped through hole 4. An arc-shaped plate 6 is fixedly connected to the rocker arm 5. A sliding groove 7 is provided in the arc-shaped plate 6. A side plate 8 is fixedly installed on one side of the device housing 1. A motor 9 is fixedly installed on the side of the side plate 8 near the partition 3. An L-shaped rod 10 is fixedly installed in the device housing 1 at the output end of the motor 9. A connecting shaft 11 is rotatably connected to the L-shaped rod 10. The connecting shaft 11 is slidably connected to the sliding groove 7. A bracket 12 is fixedly installed at one end of the rocker arm 5 that extends into the device housing 1. A plurality of container fixing frames 14 are evenly provided on the bracket 12.

[0027] As described above, the vessel is placed in the vessel fixing frame 14, and after placement, the protective cover 2 is closed; the motor 9 is controlled to work, driving the L-shaped rod 10 to rotate, which in turn drives the connecting shaft 11 to rotate, causing the arc plate 6 to move back and forth, and the rocker arm 5 to move in an arc within the strip-shaped through hole 4, thereby realizing the arc plate 6 to move back and forth in an arc with the rocker arm 5 and the strip-shaped through hole 4 as the center, so that the bracket 12 can move back and forth in an arc, thereby shaking the vessel in the vessel fixing frame 14.

[0028] As a technical optimization of this utility model, the vessel fixing frame 14 includes a connecting rod 1401 fixedly connected to the bracket 12, an arc frame 1402 fixedly connected to the connecting rod 1401, a plurality of connecting rods 1403 evenly fixedly connected to the lower side of the arc frame 1402, a base plate 1404 fixedly installed between the connecting rods 1403, a plurality of grooves 1405 evenly provided in the arc frame 1402, a spring 1406 fixedly installed in the groove 1405, and a telescopic rod 1407 fixedly installed at the other end of the spring 1406.

[0029] As described above, when the vessel is inserted through the arc-shaped frame 1402, the base plate 1404 comes into contact with the vessel. During the descent of the vessel, it will first push open the telescopic rod 1407 inside the arc-shaped frame 1402 and compress the spring 1406. The elastic force of the spring 1406 will apply pressure to the vessel between the telescopic rods 1407, thus providing a relatively stable working environment for the vessel. The separately set connecting rods 1403 make it easy for researchers to see whether the bottom of the vessel is in contact with the base plate 1404. Only when the vessel is in contact with the base plate 1404 can a more stable shaking environment be obtained.

[0030] As a technical optimization of this utility model, a ball bearing 1408 is rotatably connected to the telescopic rod 1407;

[0031] As can be seen from the above description, the ball bearing 1408 can reduce the friction between the vessel and the telescopic rod 1407, resulting in less wear on the vessel.

[0032] As a technical optimization of this utility model, the telescopic rod 1407 is slidably connected to the groove 1405;

[0033] As can be seen from the above description, the sliding connection method makes the movement of the telescopic rod 1407 more stable.

[0034] As a technical optimization of this utility model, the base plate 1404 is made of rubber material;

[0035] As can be seen from the above description, the rubber material can effectively dampen the impact pressure when the vessel comes into contact with the base plate 1404, thus providing effective protection for the vessel.

[0036] As a technical optimization of this utility model, the rocker arm 5 is slidably connected to the strip-shaped through hole 4;

[0037] As can be seen from the above description, the sliding connection method can effectively provide the working stability of the joystick 5.

[0038] As a technical optimization of this utility model, a reinforcing ring 13 is fixedly installed between the L-shaped rod 10 and the output end of the motor 9;

[0039] As can be seen from the above description, the reinforcing ring 13 can improve the firmness of the connection between the motor 9 and the L-shaped rod 10.

[0040] The working process of this utility model is as follows:

[0041] Open the protective cover 2 and place the vessel inside the vessel fixing frame 14. Use the elastic force of the spring 1406 to fix and hold the vessel with the telescopic rod 1407 and the ball bearing 1408. After placing the vessel, close the protective cover 2. Control the motor 9 to work, drive the L-shaped rod 10 to rotate, drive the connecting shaft 11 to rotate, so that the arc plate 6 moves back and forth, and the rocker arm 5 moves in an arc within the strip-shaped through hole 4. This allows the arc plate 6 to move back and forth in an arc with the rocker arm 5 and the strip-shaped through hole 4 as the center, so that the bracket 12 moves back and forth in an arc, thereby shaking the vessel inside the vessel fixing frame 14.

[0042] The present invention and its embodiments have been described above. This description is not restrictive, and the specific embodiments shown are only one of the embodiments of the present invention. The actual structure is not limited to this. In short, if a person skilled in the art is inspired by this description and designs a similar structure and embodiment without departing from the inventive spirit of the present invention, such design should fall within the protection scope of the present invention.

Claims

1. An exosome culture device comprising a device housing, characterized by: The device shell is hingedly connected with a protective cover, and an exosome shaking fixing assembly is arranged in the device shell; the exosome shaking fixing assembly comprises a partition plate arranged in the device shell, a strip-shaped through hole is arranged on the partition plate, a rocker is hingedly connected in the strip-shaped through hole, an arc-shaped plate is fixedly connected on the rocker, a sliding groove is arranged in the arc-shaped plate, a side plate is fixedly installed on one side of the device shell, a motor is fixedly installed on the side of the partition plate of the side plate, an L-shaped rod is fixedly installed on the output end of the motor extending into the device shell, a connecting shaft is rotatably connected on the L-shaped rod, the connecting shaft is slidably connected with the sliding groove, a support is fixedly installed on one end of the rocker extending into the device shell, and a plurality of vessel fixing frames are uniformly arranged on the support.

2. The exosome culture device of claim 1, wherein: The vessel fixing frame comprises a connecting rod fixedly connected with the support, an arc-shaped frame is fixedly connected on the connecting rod, a plurality of connecting rods are fixedly and uniformly connected on the lower side of the arc-shaped frame, a bottom plate is fixedly installed between the connecting rods, a plurality of recesses are uniformly arranged in the arc-shaped frame, springs are fixedly installed in the recesses, and telescopic rods are fixedly installed on the other end of the springs.

3. The exosome culture device of claim 2, wherein: A rolling ball is rotatably connected on the telescopic rod.

4. The exosome culture device of claim 2, wherein: The telescopic rod is slidably connected with the recess.

5. The exosome culture device of claim 2, wherein: The bottom plate is made of rubber material.

6. The exosome culture device of claim 1, wherein: The rocker is slidably connected with the strip-shaped through hole.

7. The exosome culture device of claim 1, wherein: A reinforcing ring is fixedly installed between the L-shaped rod and the output end of the motor.

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