Double-layer immunochromatography test paper and kit for detecting components of dairy products

Abstract

The utility model relates to the technical field of dairy component detection, in particular to double-layer immunochromatography test paper for detecting dairy components, which comprises a bearing plate, a connecting plate integrally formed at the front end of the bearing plate, a clamping block integrally formed at the rear end of the bearing plate and a two-dimensional code area arranged on the left side of the upper end of the bearing plate. The upper end of the bearing plate is fixedly connected with an upper layer detection main body, and the lower end of the bearing plate is fixedly connected with a lower layer detection main body. The utility model further discloses a kit containing the double-layer immunochromatography test paper for detecting the dairy components. According to the double-layer immunochromatography test paper and the kit for detecting the components of the dairy products, disclosed by the utility model, through a unique structural design and an advanced marking technology, efficient and accurate detection on various components of the dairy products is realized, and the operation experience of a user and the data management efficiency are remarkably improved.

Description

Technical Field

[0001] This utility model relates to the field of dairy component detection technology, and in particular to a double-layer immunochromatographic test strip and reagent kit for detecting dairy components. Background Technology

[0002] In the current field of dairy product quality control and food safety monitoring, traditional single-layer immunochromatographic test strips, due to their limitations in sensitivity and specificity, are insufficient to meet the diverse and complex needs for detecting dairy product components. These traditional test strips typically only detect a single component and are prone to false positives and false negatives when processing complex samples, affecting the accuracy and reliability of the detection. Furthermore, existing testing tools also suffer from shortcomings in user experience and ease of operation, such as difficulty in simultaneously detecting multiple components, inconvenient data management, and complex operation. Therefore, we propose a double-layer immunochromatographic test strip and kit for detecting dairy product components. Utility Model Content

[0003] The main purpose of this invention is to provide a double-layer immunochromatographic test strip and reagent kit for detecting dairy product components, which can effectively solve the problems in the background art.

[0004] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0005] A double-layer immunochromatographic test strip for detecting dairy product components includes a carrier plate, a connecting plate integrally formed at the front end of the carrier plate, a locking block integrally formed at the rear end of the carrier plate, a QR code area on the upper left side of the connecting plate, and pinch grooves on the upper right and lower right sides of the carrier plate, which are symmetrically arranged. An upper detection body is fixedly connected to the upper end of the carrier plate, and a lower detection body is fixedly connected to the lower end of the carrier plate.

[0006] The upper end of the connecting plate is provided with a slot for use with the card block. The card block is engaged in the slot to connect two adjacent bearing plates together.

[0007] Preferably, the upper detection body includes a sample pad, a conjugate pad, a chromatography membrane, and an absorbent pad. The lower surface of the chromatography membrane is fixedly attached to the upper end of the support plate. One end of the upper surface of the chromatography membrane is overlapped and connected to the conjugate pad, and the other end is overlapped and connected to the absorbent pad. The other end of the conjugate pad is overlapped and connected to the sample pad.

[0008] Preferably, the chromatography membrane is provided with a detection line and a control line, and the binding pad is coated with bovine IgG or goat IgG labeled with gold nanoparticles.

[0009] By adopting the above technical solution, the application of nano-gold particle labeling technology further improves the detection sensitivity, enabling even small concentrations of target antigens to be effectively identified.

[0010] Preferably, the particle size of the gold nanoparticles is 20-50 nm, the detection line is a detection line coated with anti-bovine IgG or anti-goat IgG, and the control line is a control line coated with Staphylococcus aureus protein A.

[0011] By adopting the above technical solution, the double-layer structure not only improves the throughput of detection, but also significantly enhances the accuracy and reliability of the detection results.

[0012] Preferably, the chromatography membrane is a nitrocellulose membrane.

[0013] By adopting the above technical solution, the sample exhibits excellent adsorption performance and fluidity, ensuring stability and consistency during the chromatography process.

[0014] Preferably, the absorbent pad is equipped with an RFID radio frequency card.

[0015] By adopting the above technical solution, the RFID radio frequency card set on the absorbent pad can record the detection data in real time, which facilitates subsequent data tracking and analysis, and greatly improves the user experience and work efficiency.

[0016] Preferably, the structure of the lower detection body is the same as that of the upper detection body, and the connection method between the lower detection body and the support plate is the same as that between the upper detection body and the support plate.

[0017] By adopting the above technical solution, the upper detection subject and the lower detection subject target different antigens, such as bovine IgG and goat IgG.

[0018] This invention also provides a kit containing the above-mentioned double-layer immunochromatographic test strip for detecting dairy product components.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] 1. The design of the carrier plate fully considers the actual operating needs of users. The one-piece molded connecting plate at the front and the one-piece molded locking block at the rear allow multiple test strips to be easily connected together to form a whole, facilitating storage and transportation. At the same time, the QR code area on the carrier plate can quickly provide product information and instructions for use, simplifying the user's data management process. The symmetrical design of the two gripping slots at the top and bottom allows users to hold and operate the test strips more easily, avoiding errors caused by improper operation.

[0021] 2. This invention utilizes a unique double-layer immunochromatographic test strip to simultaneously detect multiple components in dairy products. The upper and lower detection layers target different antigens, each with its own detection line and control line on its respective chromatographic membrane. This double-layer structure not only increases throughput but also significantly enhances the accuracy and reliability of the results. The application of gold nanoparticle labeling technology further improves detection sensitivity, enabling the effective identification of even minute concentrations of target antigens. Furthermore, the nitrocellulose membrane, as the chromatographic membrane material, possesses excellent adsorption properties and fluidity, ensuring the stability and consistency of the sample during the chromatographic process. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the connection structure of the double-layer immunochromatographic test strip for detecting dairy product components according to this utility model;

[0023] Figure 2 This is a first-view structural schematic diagram of the overall structure of the double-layer immunochromatographic test strip for detecting dairy product components according to this utility model.

[0024] Figure 3 This is a second-view schematic diagram of the overall structure of the double-layer immunochromatographic test strip for detecting dairy product components according to this utility model.

[0025] Figure 4 This is a schematic diagram of the upper detection body of the double-layer immunochromatographic test strip for detecting dairy product components according to this utility model.

[0026] In the diagram: 1. Support plate; 2. Connecting plate; 21. Slot; 3. Card block; 4. QR code area; 5. Grip groove; 6. Upper detection body; 61. Sample pad; 62. Binding pad; 63. Chromatography membrane; 631. Detection line; 632. Quality control line; 64. Absorbent pad; 641. RFID radio frequency card; 7. Lower detection body. Detailed Implementation

[0027] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0028] In the description of this utility model, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," and "the other end," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are used only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model. In addition, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0029] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installed," "equipped with," and "connected," etc., should be interpreted broadly. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium; it can be a connection within two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0030] Please see Figure 1-4 This utility model provides a technical solution:

[0031] A double-layer immunochromatographic test strip for detecting dairy product components includes a carrier plate 1, a connecting plate 2 integrally formed at the front end of the carrier plate 1, a locking block 3 integrally formed at the rear end of the carrier plate 1, a QR code area 4 on the upper left side of the carrier plate 1, and pinch grooves 5 on both the upper and lower right sides of the carrier plate 1, with the two pinch grooves 5 arranged symmetrically. An upper detection body 6 is fixedly connected to the upper end of the carrier plate 1, and a lower detection body 7 is fixedly connected to the lower end of the carrier plate 1.

[0032] In this embodiment, the upper end of the connecting plate 2 is provided with a slot 21 that works with the card block 3. The card block 3 is engaged in the slot 21 to connect two adjacent bearing plates 1 together.

[0033] Through the above scheme: the design of the carrier plate 1 fully considers the actual operating needs of users, and the front-end integrated connecting plate 2 and the rear-end integrated card block 3 make it easy to connect multiple test strips together to form a whole, which is convenient for storage and transportation.

[0034] In this embodiment, the upper detection body 6 includes a sample pad 61, a conjugate pad 62, a chromatography membrane 63, and an absorbent pad 64. The lower surface of the chromatography membrane 63 is fixedly attached to the upper end of the support plate 1. One end of the upper surface of the chromatography membrane 63 is overlapped and connected to the conjugate pad 62, and the other end is overlapped and connected to the absorbent pad 64. The other end of the conjugate pad 62 is overlapped and connected to the sample pad 61. The chromatography membrane 63 is provided with a detection line 631 and a control line 632. The conjugate pad 62 is coated with bovine IgG or goat IgG labeled with gold nanoparticles. The nano-gold particles have a particle size of 20-50nm; the detection line 631 is a detection line coated with anti-bovine IgG or anti-goat IgG; the quality control line 632 is a quality control line coated with Staphylococcus aureus protein A; the chromatography membrane 63 is a nitrocellulose membrane; an RFID radio frequency card 641 is installed on the absorbent pad 64; the structure of the lower detection body 7 is the same as that of the upper detection body 6, and the connection method between the lower detection body 7 and the support plate 1 is the same as that between the upper detection body 6 and the support plate 1.

[0035] Through the above scheme, the upper detection body 6 and the lower detection body 7 target different antigens, such as bovine IgG and goat IgG, respectively, and each has a detection line 631 and a control line 632 set on its respective chromatographic membrane 63. This double-layer structure not only improves the throughput of detection but also significantly enhances the accuracy and reliability of the detection results. The application of nano-gold particle labeling technology further improves the detection sensitivity, enabling the effective identification of even small concentrations of target antigens. In addition, the nitrocellulose membrane, as a chromatographic membrane material, has good adsorption properties and fluidity, ensuring the stability and consistency of the sample during the chromatography process. The RFID radio frequency card 641 set on the absorbent pad 64 can record the detection data in real time, facilitating subsequent data tracking and analysis, greatly improving the user experience and work efficiency.

[0036] This invention also provides a kit containing the above-mentioned double-layer immunochromatographic test strip for detecting dairy product components.

[0037] It should be noted that this utility model is a double-layer immunochromatographic test strip and kit for detecting dairy product components. During use, the user first adds the dairy product sample to be tested to the sample pad 61 of the upper detection body 6 and the sample pad 61 of the lower detection body 7. The sample pad 61, as the first step of sample loading, absorbs and evenly distributes the sample solution. The sample solution then flows from the sample pad 61 to the conjugate pad 62, where it reacts with the antibody labeled with gold nanoparticles pre-coated on the conjugate pad. In the conjugate pad 62, the gold nanoparticle-labeled bovine IgG or goat IgG antibody specifically binds to the target antigen in the sample, such as bovine IgG or goat IgG. This specific binding forms an antigen-antibody complex. These complexes continue to migrate towards the chromatography membrane 63 with the sample solution. The sample solution carries the antigen-antibody complexes forward along the chromatography membrane 63. This membrane is usually a nitrocellulose membrane, which has good adsorption properties and fluidity, ensuring the stability and consistency of the sample solution. During chromatography, the sample solution passes sequentially through the test line 631 and the control line 632. When the sample solution flows through the test line 631, the antigen in the antigen-antibody complex binds secondary to the anti-bovine IgG or anti-goat IgG antibody immobilized on the test line. If the sample contains the target antigen, these antigens will bind to the antibody on the test line, forming a visible color reaction. This color change indicates a positive test result. Regardless of whether the sample contains the target antigen, the sample solution continues to flow to the control line 632. The control line 632 is coated with Staphylococcus aureus A protein, which can bind to the gold nanoparticle-labeled antibody in the sample solution, producing a fixed red line. The existence of this control line 632 proves the effectiveness of the entire detection process and ensures the reliability of the test results. The absorbent pad 64 is located at the end of the chromatography membrane 63 and is responsible for absorbing excess sample solution to prevent liquid backflow from affecting the test results 6.1. In addition, the RFID radio frequency card 641 set on the absorbent pad 64 can record the detection data in real time, which facilitates subsequent data tracking and analysis. Users can obtain product information and instructions for use by scanning the QR code area 4, which further simplifies the operation process.

[0038] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. Double immunochromatographic test strip for the detection of dairy ingredients comprising a support plate (1), characterized in that: The front end of the bearing plate (1) is integrally formed with a connecting plate (2), the rear end of the bearing plate (1) is integrally formed with a clamping block (3), the left side of the upper end of the bearing plate (1) is provided with a two-dimensional code area (4), the right side of the upper end of the bearing plate (1) and the right side of the lower end of the bearing plate (1) are provided with a pinch groove (5), and the upper and lower pinch grooves (5) are symmetrically arranged, the upper end of the bearing plate (1) is fixedly connected with an upper layer detection main body (6), and the lower end of the bearing plate (1) is fixedly connected with a lower layer detection main body (7). The upper end of the connecting plate (2) is provided with a clamping groove (21) matched with the clamping block (3), and the clamping block (3) is clamped in the clamping groove (21) to connect two adjacent bearing plates (1) together.

2. The double immunochromatographic test strip for detecting dairy components according to claim 1, characterized in that: The upper layer detection main body (6) comprises a sample pad (61), a binding pad (62), a chromatography membrane (63) and a water absorption pad (64), the lower surface of the chromatography membrane (63) is fixedly attached to the upper end of the bearing plate (1), one end of the upper surface of the chromatography membrane (63) is connected with the binding pad (62), and the other end of the upper surface of the chromatography membrane (63) is connected with the water absorption pad (64), and the other end of the binding pad (62) is connected with the sample pad (61).

3. The double immunochromatographic test strip for detecting dairy components according to claim 2, characterized in that: The chromatography membrane (63) is provided with a detection line (631) and a quality control line (632), and the binding pad (62) is coated with nano-gold particle labeled bovine IgG or goat IgG.

4. The double immunochromatographic test strip for detecting dairy components according to claim 3, characterized in that: The particle size of the nano-gold particle is 20-50nm, the detection line (631) is a detection line coated with anti-bovine IgG or anti-goat IgG, and the quality control line (632) is a quality control line coated with Staphylococcus aureus A protein.

5. The double immunochromatographic test strip for detecting dairy components according to claim 2, characterized in that: The chromatography membrane (63) is a nitrocellulose membrane.

6. The double immunochromatographic test strip for detecting dairy components according to claim 2, characterized in that: The water absorption pad (64) is provided with an RFID radio frequency card (641).

7. The double immunochromatographic test strip for detecting dairy components according to claim 1, characterized in that: The structure of the lower layer detection main body (7) is the same as that of the upper layer detection main body (6), and the connection mode of the lower layer detection main body (7) and the bearing plate (1) is the same as that of the upper layer detection main body (6) and the bearing plate (1).

8. A kit containing the double-layer immunochromatographic test paper for detecting dairy product components according to any one of claims 1 to 7.

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