Cell proliferation activity detection device

Abstract

The utility model discloses a cell proliferation activity detection device, which comprises a cell proliferation observer and a collection box, a discharge hose is fixed at the bottom end of the collection box in a penetrating manner, one end of the discharge hose is connected with the cell proliferation observer, and a support frame is arranged on the outer side of the collection box. Two supporting rotating shafts are rotationally inserted into the inner side of the top end of the supporting frame, a separation rotating drum is detachably installed between the two supporting rotating shafts, a fixing plate is fixed to the outer side of the supporting frame, and a rotating motor is fixed to the upper surface of the fixing plate. According to the utility model, the supporting rotating shaft and the separation rotating drum are driven by the rotating motor to carry out transportation rotation, so that a cell mixture added into the separation rotating drum flows, the flowing cell mixture impacts the filter plate to generate pressure, and the centrifugal force generated when the separation rotating drum rotates is utilized to accelerate cells to penetrate through the filter plate; the separation and collection efficiency of the cells is improved, and the cells cannot be damaged.

Description

Technical Field

[0001] This utility model relates to the field of cell proliferation activity detection technology, specifically a cell proliferation activity detection device. Background Technology

[0002] Cell proliferation activity assay is an experimental method for assessing the ability and rate of cell proliferation. It reflects the growth status and activity of cells by analyzing changes in the number of cells during division. This assay has wide applications in cell biology, tumor biology, molecular biology, and pharmacokinetics. The purpose of cell proliferation activity assay is mainly to assess the growth status and health of cells, support drug development and screening, and study disease mechanisms and prognosis. The process of cell proliferation activity assay is divided into cell isolation and collection and post-collection observation and recording.

[0003] However, when detecting cell proliferation activity and collecting cells, it is necessary to wait for the cells to undergo natural filtration and separation, which takes a long time, or to separate the cells by pressure filtration, which can damage the cells. Therefore, this does not meet the current needs. To address this, we propose a cell proliferation activity detection device. Utility Model Content

[0004] The purpose of this invention is to provide a cell proliferation activity detection device to solve the problems mentioned in the background art, such as the need to wait for cells to undergo natural filtration and separation when detecting cell proliferation activity and collecting cells, which takes a long time, or the need to use pressure filtration to separate cells, which can lead to cell damage.

[0005] To achieve the above objectives, this utility model provides the following technical solution: a cell proliferation activity detection device, comprising a cell proliferation observation instrument and a collection box, wherein a discharge hose is fixedly inserted through the bottom of the collection box, one end of the discharge hose is connected to the cell proliferation observation instrument, a support frame is provided on the outside of the collection box, two support shafts are rotatably inserted into the inner side of the top of the support frame, a separation rotating cylinder is detachably installed between the two support shafts, a fixing plate is fixed on the outside of the support frame, a rotary motor is fixed on the upper surface of the fixing plate, the output shaft end of the rotary motor is connected to one of the support shafts by a fixing pin, and the separation rotating cylinder is hollow inside and filter plates are detachably installed at both ends.

[0006] Preferably, the separating drum has feeding ports on both sides, and the inner side of the feeding port is inlaid with a baffle plate, which is fan-shaped.

[0007] Preferably, positioning holes are provided above and below the feeding port, and reinforcing strips are provided on the outer side of the baffle.

[0008] Preferably, both ends of the reinforcing strip are fixed with reinforcing components facing the surface of the separating drum. The reinforcing components are snapped into the inside of the positioning hole, and the depth of the positioning hole is half the wall thickness of the separating drum.

[0009] Preferably, one end of the friction plate is fixed to the reinforcing strip, the end of the friction plate away from the reinforcing strip is inclined outward, and the friction plate is made of spring steel.

[0010] Compared with the prior art, the beneficial effects of this utility model are:

[0011] This invention uses a rotary motor to drive a support shaft and a separation drum for transport and rotation, causing the cell mixture added into the separation drum to flow. The flowing cell mixture impacts the filter plate and generates pressure. The centrifugal force generated when the separation drum rotates accelerates the cells through the filter plate, improving the efficiency of cell separation and collection without damaging the cells. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0013] Figure 2 This is a schematic diagram of the structure of the separating rotating drum of this utility model;

[0014] Figure 3 This is an exploded view of the separating rotating drum and baffle of this utility model;

[0015] Figure 4 for Figure 3 Enlarged view of the structure at point A in the middle.

[0016] In the diagram: 1. Collection box; 2. Support frame; 3. Support shaft; 4. Rotary motor; 5. Discharge hose; 6. Separating drum; 7. Filter plate; 8. Baffle; 9. Reinforcing strip; 10. Feed port; 11. Positioning hole; 12. Reinforcing components. Detailed Implementation

[0017] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0018] like Figures 1 to 4As shown, a cell proliferation activity detection device includes a cell proliferation observer and a collection box 1. A discharge hose 5 is fixed through the bottom of the collection box 1, and one end of the discharge hose 5 is connected to the cell proliferation observer. A support frame 2 is provided on the outside of the collection box 1. Two support shafts 3 are rotatably inserted into the inner side of the top of the support frame 2. A separation drum 6 is detachably installed between the two support shafts 3. A fixing plate is fixed on the outside of the support frame 2. A rotary motor 4 is fixed on the upper surface of the fixing plate. The output shaft end of the rotary motor 4 is connected to one of the support shafts 3 through a fixing pin. The separation drum 6 is hollow inside and filter plates 7 are detachably installed at both ends. A cell mixture is added into the separation drum 6. Then, the rotary motor 4 is started to start the separation drum 6 at the top of the support shaft 3 to rotate at a uniform speed. The rotation speed of the separation drum 6 does not exceed the maximum speed of cell centrifugation, so that the cell mixture flows and impacts the filter plate 7, accelerating the separation of cells from the cell mixture.

[0019] Both ends of the separating drum 6 are provided with feeding ports 10. A baffle 8 is embedded in the inner side of the feeding port 10. The baffle 8 is fan-shaped. Positioning holes 11 are provided above and below the feeding port 10. A reinforcing strip 9 is provided on the outer side of the baffle 8. Reinforcing components 12 are fixed on the surfaces of the two ends of the reinforcing strip 9 facing the separating drum 6. The reinforcing components 12 are snapped into the inner side of the positioning holes 11. The depth of the positioning holes 11 is half the wall thickness of the separating drum 6. The reinforcing strip 9 provides pressure to the baffle 8 pointing towards the inside of the separating drum 6, so that the baffle 8 will not easily fall off the inner side of the feeding port 10 after installation, thus preventing the cell mixture from detaching from the feeding port 10.

[0020] The reinforcing component 12 includes multiple friction plates arranged in a circular array. There is a gap between adjacent friction plates. One end of the friction plate is fixed to the reinforcing strip 9. The end of the friction plate away from the reinforcing strip 9 is inclined outward. The friction plate is made of spring steel. After the multiple friction plates arranged in a circular array enter the inner side of the positioning hole 11, the unfixed ends of the friction plates will approach each other. The friction force of this end of the friction plate against the inner wall of the positioning hole 11 increases, thereby ensuring that the reinforcing strip 9 presses the baffle 8 tightly in the feeding port 10, and that the separating drum 6 will not fall off the outside of the baffle 8 during rotation.

[0021] Working principle: First, remove the reinforcing strip 9 from the outside of the baffle 8 and the separating drum 6. Then, remove the baffle 8 from the inside of the feeding port 10. Add the cell mixture to be separated into the separating drum 6 through the feeding port 10. After the cell mixture is added, reset the baffle 8. Then, insert the reinforcing component 12 at the end of the reinforcing strip 9 into the inside of the positioning hole 11, ensuring that the reinforcing strip 9 fits against the baffle 8 and the separating drum 6. Then, turn on the power to the rotary motor 4 and start it. The rotary motor 4 drives the support shaft 3 and the separating drum 6 to rotate at a constant speed. When the separating drum 6 rotates, the two... The positions of the cells at each end are exchanged in real time. During this process, the cell mixture flows inside the separation drum 6. The flowing cell mixture impacts the filter plate 7, and the cells are squeezed towards the filter plate 7 and pass through the filter plate 7. At the same time, as the cells adhere to the filter plate 7, the rotation of the separation drum 6 generates a centrifugal force on the cells. This centrifugal force causes the cells to flow towards the filter plate 7 and pass through the filter plate 7, accelerating the cell separation time, shortening the operation time, and thus improving the detection efficiency. The separated cells are collected in the collection box 1 and transported to the cell proliferation observation instrument through the discharge hose 5 for observation and recording.

[0022] It will be apparent to those skilled in the art that this invention is not limited to the details of the exemplary embodiments described above, and that it can be implemented in other specific forms without departing from the spirit or essential characteristics of this invention. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of this invention is defined by the appended claims rather than the foregoing description. Thus, it is intended that all variations falling within the meaning and scope of equivalents of the claims be included within this invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A cell proliferation activity detection device comprising a cell proliferation observation instrument and a collection box (1), characterized in that: The collecting box (1) bottom end through fixed with discharge hose (5), one end of the discharge hose (5) is connected with cell proliferation observation instrument, the outside of the collecting box (1) is equipped with support frame (2), the top end of the support frame (2) inside rotatable plug-in has two support shafts (3), two support shafts (3) between detachable mounting has separation rotary drum (6), the outside of the support frame (2) is fixed with fixed plate, the upper surface of the fixed plate is fixed with rotary motor (4), the output shaft end of the rotary motor (4) is connected with one of the support shafts (3) through fixed pin, the inside of the separation rotary drum (6) is hollow and both ends are detachably mounted with filter plate (7).

2. The cell proliferation viability detection device according to claim 1, wherein: Both ends of the separation rotary drum (6) are provided with feeding port (10), the inner side of the feeding port (10) is inlaid with baffle (8), the baffle (8) is fan-shaped.

3. The cell proliferation viability assay device of claim 2, wherein: The upper and lower of the feeding port (10) are provided with positioning hole (11), the outer side of the baffle (8) is provided with reinforcing strip (9).

4. The cell proliferation viability assay device of claim 3, wherein: The surface of the two ends of the reinforcing strip (9) towards the separation rotary drum (6) is fixed with reinforcing assembly (12), the reinforcing assembly (12) is clamped in the inner side of the positioning hole (11), and the depth size of the positioning hole (11) is half of the wall thickness size of the separation rotary drum (6).

5. A device for detecting cell proliferation activity according to claim 4, wherein: The reinforcing assembly (12) comprises a plurality of friction plates, the plurality of friction plates are circularly arranged, and there is a gap between adjacent two friction plates.

6. A device for detecting cell proliferation activity according to claim 5, wherein: One end of the friction plate is fixed with the reinforcing strip (9), and the end of the friction plate away from the reinforcing strip (9) is inclined outward, and the material of the friction plate is spring steel.

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