Mixing device for cell culture

Abstract

The utility model discloses a mixing device for cell culture, and mainly relates to the field of cell culture. Comprising a base and a culture cylinder, the top of the culture cylinder is movably connected with a top cover, the bottom of the culture cylinder is rotatably connected to the base, a driving cavity is formed in the base, a rotating seat is rotatably connected in the driving cavity, a fixing column is arranged at the bottom of the culture cylinder, and a power assembly for driving the rotating seat to rotate is arranged in the driving cavity; ejector rods are symmetrically and slidably connected to the base in the vertical direction, the tops of the ejector rods make contact with the bottom of the culture cylinder, and driving blocks for driving the ejector rods to slide in the vertical direction are symmetrically arranged in the driving cavity. The cell culture device has the beneficial effects that the technical problem that cells are easily damaged by mechanical stirring during cell culture so as to improve the culture failure rate and difficulty can be solved, the cell mixing efficiency is improved by driving the rotation and vertical movement of the culture cylinder, the possible damage to the cells is reduced, the integrity of the cells is ensured, and the cell culture efficiency is improved. And the success rate of cell culture is improved.

Description

TECHNICAL FIELD

[0001] The utility model relates to the field of cell culture, specifically a kind of mixing device for cell culture. BACKGROUND

[0002] Cell culture refers to simulating in-vivo environment (sterile, suitable temperature, pH and certain nutritional conditions, etc.) in vitro, so that it survives, grows, reproduces and maintains the main structure and function, such as NKT cells in T lymphocytes, which occupies a key position in the body's immune regulation network. They can quickly respond to antigen stimulation, coordinate innate immunity and adaptive immune response, and play an indispensable role in maintaining immune balance, so under the promotion of research and clinical transformation needs, in-vitro culture of NKT cells has become a research hotspot. However, NKT cells have very strict requirements for the culture environment. In order to improve the mixing effect of cells and nutrients during the culture process, the traditional bioreactor is prone to use direct contact mechanical stirring to mix cells. However, mechanical stirring or improper operation can easily cause damage or contamination of NKT cells, damage the integrity of the cell membrane, and thus affect the activity and function of the cells, resulting in a high risk of failure of the existing bioreactor for cell culture for NKT cell culture, increasing the difficulty of cell culture. SUMMARY

[0003] The utility model aims at providing a kind of mixing device for cell culture, it can solve the technical problem that mechanical stirring is easy to damage cell during cell culture and thus improve the failure rate and difficulty of culture, improve the efficiency of cell mixing by the drive of the rotation and vertical movement of culture cylinder, reduce the damage that can be caused to cells, ensure the integrity of cells, and improve the success rate of cell culture.

[0004] To achieve the above-mentioned purpose, the utility model realizes the following technical scheme:

[0005] A kind of mixing device for cell culture, including base and culture cylinder, the top of the culture cylinder is movably connected with top cover, the bottom of the culture cylinder is rotatably connected to the base, the inside of the base is provided with drive cavity, the drive cavity is rotatably connected with rotating seat, the bottom of the culture cylinder is provided with fixed column, the fixed column is vertically connected with rotating seat after penetrating through the base, the drive cavity is provided with power assembly for driving rotating seat to rotate, the base is symmetrically vertically slidably connected with top rod, the top of the top rod is in contact with the bottom of the culture cylinder, the drive cavity is symmetrically provided with drive block for driving top rod to slide vertically.

[0006] Furthermore, the power assembly includes a slide, a gear plate, and a gear. The slide is slidably connected in the drive cavity in a transverse direction. The gear plate is fixed on the slide and the gear is fixed on the rotating seat. The gear meshes with the gear plate.

[0007] Furthermore, a motor is provided inside the drive cavity, and a screw is fixed on the output shaft of the motor. The screw passes through the slide and is threadedly connected to it.

[0008] Furthermore, the drive block is symmetrically fixed on the slide table, the top of the drive block is provided with an inverted V-shaped groove, and the bottom of the top rod is slidably connected in the inverted V-shaped groove.

[0009] Furthermore, the base has an annular groove at its top and an annular plate at its bottom edge, the annular plate being slidably connected to the annular groove in a vertical direction.

[0010] Furthermore, the top of the rotating seat is provided with a sliding groove, the fixed column is slidably connected in the sliding groove along the vertical direction, the sliding groove is provided with multiple limiting grooves, and the side of the fixed column is provided with multiple limiting blocks that are slidably connected with the limiting grooves.

[0011] Furthermore, the top cover is provided with an air inlet pipe and a heating pipe, both of which extend into the interior of the culture tube.

[0012] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0013] 1. The structure of this utility model includes a culture tube and a base. The bottom of the culture tube is rotatably connected to the base. A rotating seat is rotatably connected in the drive cavity inside the base. A fixed column is provided at the bottom of the culture tube. The fixed column passes through the base and is slidably connected to the rotating seat vertically. When the rotating seat is driven to rotate by the power component in the drive cavity, the culture tube can be driven to rotate in the base under the connection of the fixed column. This enables rapid mixing of cells and nutrients in the culture tube. During the mixing process, it is not easy to damage the cells, especially NKT cells with high integrity requirements. This ensures the integrity of the cells during the culture process and greatly improves the success rate and culture efficiency of cell culture.

[0014] 2. A top rod is symmetrically and vertically slidably connected to the base. The top of the top rod contacts the bottom of the culture tube. In this structure, during the rotation of the culture tube, the top rod is driven to slide up and down repeatedly by the drive block in the drive chamber, thereby periodically lifting the culture tube upward. This allows for vertical mixing of cells and nutrients at different heights, further improving the mixing effect and increasing the efficiency of cell culture. Attached Figure Description

[0015] Appendix Figure 1 This is a three-dimensional structural diagram of the present invention.

[0016] Appendix Figure 2 This is a front view of the present invention.

[0017] Appendix Figure 3 This is an appendix to this utility model. Figure 2 A cross-sectional view along the AA direction.

[0018] Appendix Figure 4 This is an appendix to this utility model. Figure 3 Cross-sectional view along the BB direction.

[0019] Appendix Figure 5 This is an appendix to this utility model. Figure 4 A cross-sectional view along the CC direction.

[0020] Appendix Figure 6 This is a schematic diagram of the internal structure of the drive cavity of this utility model.

[0021] The labels shown in the attached diagram:

[0022] 1. Base; 2. Culture tube; 3. Top cover; 4. Drive chamber; 5. Rotating seat; 6. Fixed column; 7. Top rod; 8. Drive block; 9. Slide table; 10. Toothed plate; 11. Gear; 12. Motor; 13. Screw; 14. Inverted V-groove; 15. Annular groove; 16. Annular plate; 17. Slide groove; 18. Limiting groove; 19. Limiting block; 20. Air inlet pipe; 21. Heating tube. Detailed Implementation

[0023] The present invention will be further illustrated below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention. Furthermore, it should be understood that after reading the teachings of this invention, those skilled in the art can make various alterations or modifications to the present invention, and these equivalent forms also fall within the scope defined in this application.

[0024] Reference Figure 1 and Figure 2This utility model describes a mixing device for cell culture. The main structure includes a base 1 and a culture cylinder 2. The base 1 provides support, and the culture cylinder 2 is used for storing and culturing cells. A top cover 3 is movably connected to the top of the culture cylinder 2. Specifically, connecting flanges are provided at the top opening of the culture cylinder 2 and on the top cover 3. The two connecting flanges are connected by bolts, allowing the top cover 3 to be detachable, facilitating the addition of cultured cells and nutrients into the culture cylinder 2 after opening the top cover 3. The bottom of the culture cylinder 2 is rotatably connected to the base 1. The base 1 has a drive cavity 4 inside, and a rotating seat 5 is rotatably connected to the drive cavity 4 via a bearing. A through-hole is provided at the top of the culture tube 2. The rotating seat 5 is rotatably connected to the through-hole via a bearing. A fixing column 6 is fixed to the bottom of the culture tube 2 by welding or bolts. The fixing column 6 passes through the base 1 and slides vertically connected to the rotating seat 5. A limiting structure is provided between the fixing column 6 and the rotating seat 5 so that the fixing column 6 can only slide vertically relative to the rotating seat 5. When the rotating seat 5 rotates, the limiting structure ensures that the fixing column 6 rotates synchronously with it, thereby realizing the rotation drive of the culture tube 2. The driving cavity 4 is provided with a power component that drives the rotating seat 5 to rotate. When the rotating seat 5 rotates, the power component can drive the culture tube 2 to rotate under the connection of the fixing column 6. This structure only requires placing the culture tube 2 on the base 1 and inserting the fixing post 6 into the rotating seat 5. This allows the culture tube 2 to continuously rotate during subsequent culture, ensuring thorough mixing of cells and nutrients inside. This reduces the potential damage to cells caused by direct mechanical stirring, maintains cell integrity, and improves the efficiency and success rate of cell culture. The base 1 is symmetrically connected with vertically sliding top rods 7. Specifically, through holes symmetrically arranged on the base 1 extend into the drive cavity 4. The top rods 7 are vertically slidably connected within these through holes, with the top of the top rods 7 contacting the bottom of the culture tube 2. Specifically, a contact point is set at the top of the top rods 7. The plate or contact wheel contacts the bottom of the rotating culture cylinder 2. When the culture cylinder 2 rotates, the push rod 7 slides up and down to periodically lift the culture cylinder 2, thereby achieving vertical mixing of cells and nutrients, further improving mixing efficiency, cell culture efficiency and success rate. The drive cavity 4 is symmetrically provided with drive blocks 8 that drive the push rod 7 to slide vertically. The drive blocks 8 are used to drive the push rod 7 to slide vertically, so that the drive structure is set in the drive cavity 4 inside the base 1, and only the top of the push rod 7 contacts the bottom of the culture cylinder 2, which greatly simplifies the complexity of the mixing device and improves the convenience of movement and use.

[0025] Preferred, refer to Figure 5The power assembly includes a slide 9, a toothed plate 10, and a gear 11. The slide 9 is slidably connected to the drive cavity 4 in a transverse reciprocating motion. The toothed plate 10 is fixed to the slide 9 by welding or bolts, and the gear 11 is fixed to the rotating seat 5 by welding or bolts. The gear 11 meshes with the toothed plate 10. With this structure, when the slide 9 slides in a transverse reciprocating motion, the rotating seat 5 can be driven to rotate in the forward or reverse direction under the meshing of the toothed plate 10 and the gear 11, thus realizing the smooth rotation drive of the culture cylinder 2. The structure is simple, and the rotation drive of the culture cylinder 2 is smoother and more stable.

[0026] Preferred, refer to Figure 4 The drive cavity 4 is equipped with a motor 12. Specifically, a fixing plate is set in the drive cavity 4. The motor 12 is fixed to the fixing plate by welding or bolts. A screw 13 is fixed to the output shaft of the motor 12 by welding or bolts. The screw 13 passes through the slide table 9 and is threadedly connected to it. With this structure, when the motor 12 drives the screw 13 to rotate in both directions, it can drive the slide table 9 to slide laterally back and forth under the action of the threaded connection. Furthermore, guide grooves are symmetrically set on the two side walls of the drive cavity 4. The two sides of the slide table 9 are slidably connected in the guide grooves on both sides, making the lateral sliding drive of the slide table 9 smoother and more stable.

[0027] Preferably, the drive block 8 is symmetrically fixed to the slide table 9 by welding or bolts. The top of the drive block 8 is provided with an inverted V-shaped groove 14, and the bottom of the top rod 7 is slidably connected in the inverted V-shaped groove 14. Specifically, in order to reduce friction, a roller can be rotatably provided at the bottom of the top rod 7. The roller is rotatably connected in the inverted V-shaped groove 14. With this structure, while the slide table 9 drives the culture cylinder 2 to rotate laterally, it can also drive the drive block 8 to slide laterally, so that the inverted V-shaped groove 14 at the top of the drive block 8 contacts the roller at the bottom of the top rod 7. Since the inverted V-shaped groove 14 is high in the middle and low at both ends, it will drive the top rod 7 to slide up and down, thereby realizing the up and down movement drive of the culture cylinder 2. The same set of power structure can realize both the rotation drive of the culture cylinder 2 and the vertical sliding drive of the culture cylinder 2, which greatly simplifies the complexity of the drive structure and improves the efficiency and smoothness of the rotation and vertical sliding of the culture cylinder 2.

[0028] Preferred, refer to Figure 3 and Figure 6 The base 1 is provided with an annular groove 15, which is recessed downward from the top of the base 1. The bottom edge of the culture tube 2 is fixed with an annular plate 16 by welding or bolts. The annular plate 16 is slidably connected in the annular groove 15 in the vertical direction. The arrangement of the annular plate 16 and the annular groove 15 restricts the position of the culture tube 2 when it slides vertically, preventing the culture tube 2 from tilting when it moves up and down, and further improving the stability of the culture tube 2 structure.

[0029] Preferably, the top of the rotating seat 5 is provided with a sliding groove 17, which is recessed downward from the top of the rotating seat 5. The fixed column 6 is slidably connected in the sliding groove 17 in the vertical direction. The sliding groove 17 is configured to guide the up and down sliding of the fixed column 6. The sliding groove 17 is provided with multiple limiting grooves 18, which are recessed inward from the inner wall of the sliding groove 17. The side of the fixed column 6 is fixed with multiple limiting blocks 19 that are slidably connected to the limiting grooves 18 by welding or integral molding. The sliding cooperation between the limiting blocks 19 and the limiting grooves 18 allows the fixed column 6 to slide smoothly in the vertical direction relative to the rotating seat 5. At the same time, when the rotating seat 5 rotates, the fixed column 6 is driven to rotate synchronously under the cooperation of the limiting blocks 19 and the limiting grooves 18, ensuring the smoothness of the rotation and vertical sliding of the culture tube 2.

[0030] Preferably, the top cover 3 is provided with an air inlet pipe 20 and a heating pipe 21. The air inlet pipe 20 is rotatably connected to the external air supply pipeline through a rotary joint to realize continuous air supply to the inside of the culture tube 2 and ensure the continuity of the culture. Both the air inlet pipe 20 and the heating pipe 21 extend into the inside of the culture tube 2. The heating pipe 21 is used to ensure the temperature inside the culture tube 2 and improve the accuracy of the cell culture environment.

[0031] Working Principle: The structure of this utility model includes a culture cylinder 2 and a base 1. The bottom of the culture cylinder 2 is rotatably connected to the base 1. A rotating seat 5 is rotatably connected inside the driving cavity 4 of the base 1. A fixed column 6 is provided at the bottom of the culture cylinder 2. The fixed column 6 passes through the base 1 and slides vertically to the rotating seat 5. With this structure, when the rotating seat 5 is driven to rotate by the power component in the driving cavity 4, the culture cylinder 2 can be driven to rotate on the base 1 under the connection of the fixed column 6. This achieves rapid mixing of cells and nutrients in the culture cylinder 2, and the mixing process is less likely to damage cells, especially intact cells. The high-susceptibility NKT cells are not damaged, ensuring cell integrity during culture and greatly improving the success rate and efficiency of cell culture. A top rod 7 is symmetrically and vertically slidably connected to the base 1, with the top of the top rod 7 in contact with the bottom of the culture tube 2. In this structure, during the rotation of the culture tube 2, the top rod 7 is driven to slide up and down reciprocally by the driving block 8 in the driving cavity 4, thereby periodically lifting the culture tube 2 upwards. This allows for vertical mixing of cells and nutrients at different heights, further improving the mixing effect and the efficiency of cell culture.

Claims

1. A mixing device for cell culture, comprising a base (1) and a culture cylinder (2), a top cover (3) being movably connected to the top of the culture cylinder (2), characterized in that: The bottom of the culture cylinder (2) is rotatably connected to the base (1), the inside of the base (1) is provided with a driving cavity (4), the driving cavity (4) is rotatably connected with a rotating base (5), the bottom of the culture cylinder (2) is provided with a fixed column (6), the fixed column (6) is slidably connected with the rotating base (5) along the vertical direction after penetrating through the base (1), the driving cavity (4) is provided with a power assembly for driving the rotating base (5) to rotate, the base (1) is symmetrically slidably connected with a top rod (7) along the vertical direction, the top of the top rod (7) is in contact with the bottom of the culture cylinder (2), and the driving cavity (4) is symmetrically provided with a driving block (8) for driving the top rod (7) to slide along the vertical direction.

2. The mixing device for cell culture of claim 1, wherein: The power assembly comprises a sliding table (9), a toothed plate (10) and a gear (11), the sliding table (9) is slidably connected in the driving cavity (4) along the horizontal direction, the toothed plate (10) is fixed on the sliding table (9), and the gear (11) is fixed on the rotating base (5) and engaged with the toothed plate (10).

3. The mixing device for cell culture of claim 2, wherein: The driving cavity (4) is provided with a motor (12), a screw rod (13) is fixed on the output shaft of the motor (12), the screw rod (13) penetrates through the sliding table (9) and is threadedly connected with the sliding table (9).

4. The mixing device for cell culture of claim 2, wherein: The driving block (8) is symmetrically fixed on the sliding table (9), the top of the driving block (8) is provided with an inverted V-shaped groove (14), and the bottom of the top rod (7) is slidably connected in the inverted V-shaped groove (14).

5. The mixing device for cell culture of claim 1, wherein: The top of the base (1) is provided with an annular groove (15), the bottom edge of the culture cylinder (2) is provided with an annular plate (16), and the annular plate (16) is slidably connected in the annular groove (15) along the vertical direction.

6. The mixing device for cell culture of claim 1, wherein: The top of the rotating base (5) is provided with a sliding groove (17), the fixed column (6) is slidably connected in the sliding groove (17) along the vertical direction, a plurality of limiting grooves (18) are arranged in the sliding groove (17), and a plurality of limiting blocks (19) are arranged on the side surface of the fixed column (6) and slidably connected with the limiting grooves (18).

7. The hybrid device for cell culture of claim 1, wherein: The top cover (3) is provided with an air inlet pipe (20) and a heating pipe (21), and the air inlet pipe (20) and the heating pipe (21) extend into the inside of the culture cylinder (2).

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