Portable cleaning and antibody incubation device for floating piece type immunofluorescence staining

Abstract

The utility model discloses a portable cleaning and antibody incubation device for floating piece type immunofluorescence staining. The portable cleaning and antibody incubation device comprises a net-shaped accommodating cylinder and an extension part, wherein the accommodating barrel is cylindrical, and the bottom wall of the accommodating barrel is flush with the bottom wall of the pore plate for a certain distance; the extension part is of an annular design and is arranged at the top end of the accommodating cylinder in a winding manner; and the outer cover body is detachably arranged on the outer wall of the containing cylinder in a sleeving manner. According to the portable cleaning and antibody incubation device for floating piece type immunofluorescence staining, the containing cylinder and the outer cover bodies with different specifications are arranged, so that time and labor are saved in the processes of rinsing brain pieces and staining in the immunofluorescence staining, repeated piece picking is not needed, at least 80%-90% of experimental operation time can be saved, and the working efficiency is improved. And the integrity of the brain slice can be ensured.

Description

TECHNICAL FIELD

[0001] The utility model relates to pathological detection immunohistochemical staining technical field, especially a kind of portable washing and antibody incubation device for float sheet type immunofluorescence staining. BACKGROUND

[0002] Immunostaining is the principle of using the specific binding of antigen and antibody, detects the expression of specific protein (antigen), and is one of the most commonly used detection methods for discovering the functional changes of body in physiological or pathological state, and the most commonly used immunostaining includes immunohistochemical staining and immunofluorescence staining.

[0003] Immunofluorescence staining includes two types: patch type immunostaining and float sheet type immunostaining, and the utility model mainly aims at float sheet type immunostaining. And traditional float sheet type washing whole brain section needs antibody incubation 2-3 times and washing 10-12 times to complete a set of staining process;The process needs to pick up a section once for each washing, which is time-consuming and easy to damage the brain section.

[0004] Although the existing washing assembly has multiple separations, it is only suitable for the staining and washing process, and although multiple separation washing is convenient, antibody incubation still needs to be completed by picking up sections during staining. In addition, multiple brain sections placed in one washing device are also prone to cross-error risk.

[0005] Based on the above reasons, it is urgent to design a portable washing and antibody incubation device for float sheet type immunofluorescence staining to overcome the above problems. INVENTION CONTENTS

[0006] In view of the problems mentioned in the background art, the utility model aims to provide a portable washing and antibody incubation device for float sheet type immunofluorescence staining to solve the problems mentioned in the background art.

[0007] In order to achieve the above-mentioned purpose, the utility model mainly provides the following technical scheme:

[0008] A portable washing and antibody incubation device for float sheet type immunofluorescence staining, comprising a net-shaped containing cylinder and an extension part;Wherein:

[0009] The containing cylinder is cylindrical, and the bottom wall is flush with the hole plate bottom wall at a distance;

[0010] The extension part is designed as a ring, which is arranged around the top end of the containing cylinder;

[0011] It further comprises an outer cover, which is detachably sleeved on the outer wall of the containing cylinder.

[0012] Further, the containing cylinder comprises a bottom net and a side net arranged around the bottom net and extending upward;

[0013] The bottom net is circular or square.

[0014] Further, the bottom net and the side net are made of medical stainless steel or PET film;

[0015] The bottom net and the side net are uniformly provided with micropores for the cleaning liquid to pass through.

[0016] Further, the extension part is a ring-shaped ring, and specifications and models are printed on the ring-shaped ring.

[0017] The extension part is attached with an anti-skid silica gel pad.

[0018] Further, a handheld part is arranged on both sides of the extension part.

[0019] The handheld part is embedded in a groove arranged on the extension part, and the handheld part slides along the length direction of the groove and the limiting rod at the end is clamped in the slotted groove at the end of the groove.

[0020] Further, the cover body is designed in a cylindrical shape and comprises a bottom wall and a side wall.

[0021] The bottom wall is completely attached to the bottom net of the containing cylinder, and the height of the side wall is 1 / 3 of the height of the side net of the containing cylinder.

[0022] Compared with the prior art, the portable cleaning and antibody incubation device for the immunofluorescence staining of the floating slice has the following beneficial effects:

[0023] The portable cleaning and antibody incubation device for the immunofluorescence staining of the floating slice can save time and labor in the process of immunohistochemical slice washing and staining, does not need to repeatedly pick slices, can save at least 80%-90% of the time of experimental operation, and can ensure the integrity of the slices.

[0024] The portable cleaning and antibody incubation device for the immunofluorescence staining of the floating slice can simultaneously complete the staining and cleaning and the antibody incubation, and a plurality of slices in the same group can be cleaned and incubated and dyed.

[0025] The portable cleaning and antibody incubation device for the immunofluorescence staining of the floating slice further comprises a cover body, which can be used for incubating the primary antibody and the secondary antibody and is used on the cleaning assembly and is beneficial to the recovery and reuse of the antibody.

[0026] The portable cleaning and antibody incubation device for the floating slice type immunofluorescence staining is favorable for saving time and labor when immunofluorescence floating slices are washed, does not need to repeatedly pick slices in the cleaning process, can save at least 80-90% of the time of operation, and can guarantee the integrity of the slices.

[0027] The portable cleaning and antibody incubation device for the floating slice type immunofluorescence staining has the characteristics of simple structure and convenient and practical use, does not need complicated slice fishing and picking links, and greatly saves cleaning time.

[0028] The above description is only a summary of the technical scheme of the utility model, in order to clearly understand the technical means of the utility model, and can be implemented according to the content of the specification, the following preferred embodiments of the utility model are described in detail below. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical scheme in the embodiments of the utility model or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or the prior art description, obviously, the drawings in the following description are only some embodiments of the utility model, and for those skilled in the art, other drawings can also be obtained according to these drawings without creative labor.

[0030] Figure 1 It is the use state diagram of the utility model;

[0031] Figure 2 It is the structure schematic diagram of the utility model;

[0032] Figure 3 It is another structure schematic diagram of the utility model;

[0033] Figure 4 It is the structure schematic diagram of the embodiment of the utility model;

[0034] EXPLANATION OF REFERENCE NUMERALS:

[0035] 1, containing cylinder;11, bottom net;12, side net;

[0036] 2, epitaxial part;21, antiskid silica gel pad;22, groove body;

[0037] 3, culture dish;

[0038] 4, hand-held part;41, slot;42, limiting rod;

[0039] 5, cover body;51, bottom wall;52, side wall; DETAILED DESCRIPTION

[0040] In order to further illustrate the technical means and effects taken by the utility model to achieve the predetermined utility model purposes and the functions, the specific embodiments, structures, features and functions of the portable cleaning and antibody incubation device for immunofluorescence staining of floating slices according to the utility model will be described in detail as follows in combination with the drawings and preferred embodiments.

[0041] As shown in Figures 1-3 The portable cleaning and antibody incubation device for immunofluorescence staining of floating slices of the utility model comprises a net-shaped containing cylinder 1 and an extension part 2, wherein:

[0042] The containing cylinder 1 is cylindrical and the bottom wall 51 is flush with the hole plate bottom wall at a distance; the height of the bottom wall 51 of the containing cylinder 1 from the dish bottom is 0.5-1 mm, which ensures the normal cleaning;

[0043] The extension part 2 is annular in design and is arranged at the top end of the containing cylinder 1; the extension part 2 is placed at the top outer wall of the six-hole plate or the culture dish 3; the width of the extension part 2 is preferably 2-5 mm.

[0044] After the brain slices are cleaned, the primary antibody and the secondary antibody need to be incubated; in order to improve the use efficiency, the utility model further comprises an outer cover 5 which is detachably sleeved on the outer wall of the containing cylinder 1; the outer cover 5 not only provides a relatively closed environment for the antibody incubation process, which helps to maintain constant temperature and humidity conditions and promote the effective combination of the antibody and the sample, but also facilitates the recovery and reuse of the antibody reagent after the incubation is completed, thereby reducing the experimental cost.

[0045] It should be noted that the cleaning body of the utility model needs to be used in cooperation with devices of different sizes, and the conventional devices are 12-hole plates / six-hole plates / 6cm dishes / 10cm dishes and devices of different specifications, so the size of the cleaning body of the utility model is designed to be multi-specification, and the user can select the appropriate specification according to the needs.

[0046] In the utility model, the user places the brain slices that need to be cleaned in the containing cylinder 1, and places them in the corresponding device, whether it is a six-hole plate or a culture dish 3 of various sizes, without the cumbersome steps of fishing and picking slices; during the cleaning process, only the upper chamber needs to be taken out and placed in the lower chamber device of another device containing clean PBS liquid, which can save a lot of time.

[0047] The containing cylinder 1 of the utility model is similar to a groove and can be suitable for dishes of different devices, and the material porosity is dense, which is only suitable for water penetration, which is beneficial to save time and effort when immunofluorescence floating brain slices; during the cleaning process, the slices do not need to be repeatedly picked, at least 80-90% of the operation time can be saved, and the integrity of the brain slices can be ensured.

[0048] It should be noted that during the process of immunostaining, the slice needs to be first washed in the containing cylinder 1 containing PBS solution; since the slice is in a floating state during the washing process, it ensures that the slice can be fully and evenly contacted with the PBS buffer, thereby achieving sufficient and uniform washing. This design avoids the washing blind area caused by the slice adhering to the bottom or side of the container in the traditional method, thereby improving the washing effect.

[0049] After the PBS washing is completed, the slice does not need to be taken out of the containing cylinder 1, and antibody incubation can be directly performed; the antibody incubation solution is added to the outer cover, and the containing cylinder containing the slice is directly placed in the outer cover containing the antibody incubation solution after being washed. Since the slice is still in a floating state, the antibody incubation solution can quickly and uniformly cover the entire slice; the design of the containing cylinder 1 enables the antibody incubation solution to maintain sufficient contact with the slice within a certain time, thereby achieving effective antibody binding.

[0050] The washing and incubation processes are continuously performed in the same device without transferring the slice, thereby reducing the operation steps and potential contamination risk.

[0051] Further, the containing cylinder 1 comprises a bottom net 11 and a side net 12 arranged around the bottom net 11 and extending upward;

[0052] The bottom net 11 is circular or square; the specifications of the bottom net 11 and the side net 12 are set as needed, and the device comprises multiple groups of different specifications, which are respectively marked with specific specifications on the outer extension part 2, thereby facilitating users to take and use according to needs.

[0053] Further, the bottom net 11 and the side net 12 are made of medical stainless steel or PET film;

[0054] The bottom net 11 and the side net 12 are uniformly provided with micropores for the cleaning solution to pass through.

[0055] The bottom net 11 and the side net 12 are made of dense stainless steel net / or PET film, and the material porosity is dense, which is only suitable for water to permeate, thereby being beneficial to saving time and effort during immunofluorescence washing of the slice.

[0056] In some specific embodiments, referring to Figure 4 , the outer extension part 2 is an annular ring, and specifications and models are printed thereon;

[0057] The outer extension part 2 is attached with an anti-slip silica gel pad 21, thereby facilitating users to hold and take it to the lower chamber device of another device containing clean PBS solution.

[0058] As a preferred embodiment, referring to Figure 3 Further comprising a hand-holding part 4 arranged on both sides of the outer extension part 2;

[0059] The handheld part 4 is embedded in the groove 22 provided on the extension part 2, and the end limiting rod 42 of the handheld part 4 is clamped in the slot 41 at the end of the groove 22; in order to avoid the handheld part 4 from being separated from the groove 22, the limiting rod 42 is arranged at the end of the handheld part 4 and clamped in the slot 41, so that the handheld part 4 cannot be separated from the groove 22; when it is needed to return to the groove 22, the limiting rod 42 is clamped in the slot 41 on the other side of the groove 22.

[0060] The handheld part 4 is embedded in the groove 22 provided on the extension part 2, and the end limiting rod 42 of the handheld part 4 is clamped in the slot 41 at the end of the groove 22; in order to avoid the handheld part 4 from being separated from the groove 22, the limiting rod 42 is arranged at the end of the handheld part 4 and clamped in the slot 41, so that the handheld part 4 cannot be separated from the groove 22; when it is needed to return to the groove 22, the limiting rod 42 is clamped in the slot 41 on the other side of the groove 22.

[0061] Further, the outer cover 5 is designed in a cylindrical shape, which includes a bottom wall 51 and a side wall 52.

[0062] The bottom wall 51 is completely attached to the bottom net 11 of the containing cylinder 1, and the height of the side wall 52 is 1 / 3 of the height of the side net 12 of the containing cylinder 1; which can ensure the normal incubation of the primary antibody and the secondary antibody during the antibody incubation process; at the same time, the primary antibody and the secondary antibody reagent can be recycled and reused 1-2 times after the incubation is completed.

[0063] As a preferred embodiment, the preferred material of the outer cover 5 is polystyrene, which ensures its wide application in the field of cell culture because of its good chemical stability, resistance to erosion of various chemicals, and no release of harmful substances. In addition, polystyrene also has good transparency, which is convenient for observing the growth of cells. During the cell culture experiment, cells are usually placed on a polystyrene culture plate, and nutrients and growth factors are added to promote the growth and reproduction of cells.

[0064] The device of the utility model ingeniously integrates the steps of dyeing and cleaning and antibody incubation, and realizes the simultaneous performance of the two steps.

[0065] During the cleaning process, the brain slice is placed in the containing cylinder, and the PBS buffer solution is injected for rinsing. Since the brain slice is in a floating state, it can fully contact the cleaning liquid to achieve uniform and thorough cleaning.

[0066] After cleaning, the brain slice can be directly incubated with antibodies without being taken out. The antibody incubation liquid is added dropwise into the cleaning liquid, or added dropwise after replacing part of the cleaning liquid, to ensure that the antibodies can quickly and uniformly cover the entire brain slice.

[0067] The design of the present application not only simplifies the operation process, but also improves the experimental efficiency.

[0068] The utility model makes further description in combination with the embodiment above, but the utility model is not limited to the above implementation, within the knowledge scope of ordinary skill in the art, still can make various changes without departing from the utility model's purpose.

Claims

1. A portable cleaning and antibody incubation device for float immunofluorescence staining, characterized in that: it comprises a net containing cylinder (1) and an extension part (2); wherein: the containing cylinder (1) is cylindrical and its bottom wall (51) is flush with the hole plate bottom wall at a distance; the extension part (2) is annular in design, which is arranged around the top end of the containing cylinder (1); it further comprises an outer cover (5) which is detachably sleeved on the outer wall of the containing cylinder (1). The containing cylinder (1) comprises a bottom net (11) and a side net (12) arranged around the bottom net (11) and extending upward; the bottom net (11) is circular or square; the bottom net (11) and the side net (12) are made of medical stainless steel or PET film; the bottom net (11) and the side net (12) are uniformly provided with micropores for cleaning liquid to pass through; the extension part (2) is an annular ring, which is printed with specifications and models; the extension part (2) is attached with a non-slip silica gel pad (21); it further comprises a handheld part (4) arranged on both sides of the extension part (2); the handheld part (4) is embedded in the groove (22) provided on the extension part (2), and the handheld part (4) slides along the length direction of the groove (22) and the limiting rod (42) at the end is clamped in the slot (41) at the end of the groove (22); the outer cover (5) is cylindrical in design, which comprises a bottom wall (51) and a side wall (52). ​ ​ ​ 2. A portable washing and antibody incubation device for dip-slide immunofluorescence staining according to claim 1, characterized in that : ​ ​ 3. A portable washing and antibody incubation device for slide-based immunofluorescence staining according to claim 2, characterized in that : ​ ​ 4. The portable washing and antibody incubation device for dip-slide immunofluorescence staining according to claim 1, characterized in that : ​ ​ 5. The portable washing and antibody incubation device for dip-slide immunofluorescence staining according to claim 1, wherein : ​ ​ 6. A portable washing and antibody incubation device for dip-slide immunofluorescent staining as claimed in claim 1, wherein : ​ Said bottom wall (51) is completely adhered to the bottom web (11) of the containment cylinder (1), said side wall (52) having a height equal to that of the side web (12) of the containment cylinder (1) 1 / 3.

Images