Perforated plate for culturing zebra fish

Abstract

The utility model relates to experimental consumables, in particular to a perforated plate for zebra fish culture, which comprises a plurality of groups of culture holes, each group of culture holes comprises a plurality of culture holes, adjacent culture holes in the same group are communicated through micropores, the diameters of the micropores are matched with those of eggs of zebra fish, and the diameters of the micropores are matched with those of the eggs of the zebra fish. And when the amount of the culture medium added into the culture holes is a preset volume, the culture medium just enters the micropores. By arranging the micropores, the old culture medium does not need to be sucked away first and then the new culture medium is quantitatively added, only the new culture medium needs to be continuously added on the basis of the old culture medium until the culture medium enters the micropores, at the moment, the volume of the culture medium in the culture holes is close to the preset volume, and the excessive part can be automatically transferred to the communicated adjacent culture holes and does not need to be sucked out manually; and the roes with large direct drop cannot pass through the micropores, so that the operation difficulty of exposing and culturing the roes is reduced by adopting the structure.

Description

Technical Field

[0001] This utility model relates to experimental consumables, and in particular to a multi-well plate for zebrafish culture. Background Technology

[0002] Zebrafish share 87% genetic homology with humans, making them a prominent model organism and important experimental animals. Currently, they are widely used in evaluating the efficacy and safety of cosmetics, the efficacy and functionality of drugs, and the efficacy and safety of health foods.

[0003] When conducting animal experiments with zebrafish, it is often necessary to select a certain number of qualified zebrafish eggs as exposure samples. After egg collection, the eggs need to be transferred to six-well plates using a dropper, and then a certain amount of culture medium is added according to the number of eggs. During this process, a certain amount of old culture medium is inevitably transferred into the six-well plates when the zebrafish eggs are transferred using the dropper. To ensure that the final volume of culture medium is the same as the preset volume, the old culture medium needs to be removed and discarded before quantitatively adding new culture medium. This step is quite tedious. Utility Model Content

[0004] The purpose of this invention is to provide a multi-well plate that facilitates the separate culture of small numbers of zebrafish.

[0005] To achieve the above objectives, this utility model provides the following technical solution:

[0006] A multi-well plate for zebrafish culture includes several groups of culture wells, and each group of culture wells includes multiple wells. The adjacent culture wells in the same group are connected by micropores. The diameter of the micropores is matched with the diameter of zebrafish eggs. When the amount of culture medium added to the culture well is a preset volume, the liquid level of the culture medium is exactly tangent to the micropore.

[0007] Optionally, multiple micropores of different heights are provided, each micropore having a height that corresponds to a different preset volume, and each micropore being controllably openable or closed.

[0008] Optionally, the micropore is closed by a soft plug that fits into the micropore. The soft plug has a small-diameter through portion and a large-diameter sealing portion, the diameter of which fits the micropore.

[0009] Optionally, the bottom of the culture well is provided with a plurality of filter holes for connecting the inside and outside of the culture well, the diameter of the filter holes being matched with the diameter of the fish eggs, and the filter holes being controllably opened or closed.

[0010] Optionally, the multi-well plate for zebrafish culture includes a body and a filling assembly. The culture wells are formed in the body, and the body also includes an opening groove disposed below each culture well. The opening of the opening groove is disposed on the side of the body, and the top of the opening groove communicates with the interior of the culture well through the filter hole. The filling assembly is fitted into the opening groove and can be controllably filled into the opening groove or separated from the opening groove. The side of the filling assembly near the filter hole is provided with several flexible sealing members for opening or closing the filter hole.

[0011] Optionally, the filling assembly includes the sealing member, a smooth-surfaced closing plate, and an abutment plate. The sealing member is connected to the surface of the closing plate. The total thickness of the sealing member, the closing plate, and the abutment plate is matched to the height of the opening groove. When the filling assembly fills the opening groove, the abutment plate abuts against the inner wall of the opening groove and the side of the closing plate away from the sealing member. The sealing member abuts against the filter holes.

[0012] Optionally, the body, the closing plate, and the sealing member are all constructed as transparent structures, and the contact plate is a lamp plate that can controllably emit light toward the culture well.

[0013] Optionally, when the abutment plate fills the opening slot, a portion of the abutment plate is located outside the opening slot.

[0014] Optionally, multiple filter holes within the same culture well are closed by the same sealing member.

[0015] The beneficial effects of this invention are as follows: by setting micropores, there is no need to first remove the old culture medium and then quantitatively add the new culture medium. Instead, new culture medium is added on the basis of the old culture medium until it enters the micropores. At this time, the volume of the culture medium in the culture well is close to the preset volume, and the excess can be automatically transferred to the connected adjacent culture wells without manual removal. Larger fish eggs cannot pass through the micropores. This structure reduces the difficulty of exposing fish eggs for culture.

[0016] Furthermore, by setting multiple sets of closable micropores, the porous plate can be adapted to various different and commonly used preset volumes.

[0017] Furthermore, closing the micropores with a soft plug is simple and cost-effective. The smaller diameter of the through-hole helps reduce the difficulty of pushing the soft plug into the micropore.

[0018] Furthermore, by setting filter holes, it is easy to separate the old culture medium, which helps to quickly remove the old culture medium when the culture medium needs to be replaced in exposed culture.

[0019] Furthermore, by providing opening slots connected to each culture well and filling components that cooperate with the opening slots, the opening and closing of the filter holes is facilitated. Using flexible sealing components to plug the filter holes helps reduce the risk of culture medium leakage.

[0020] Furthermore, by setting a closing plate and an abutment plate, the sealing component does not need to be pushed to reach the bottom of the filter hole. Instead, after the sealing component reaches the corresponding position with the closing plate, an abutment plate with a high fit and smooth surface is inserted under the closing plate to make the sealing component abut against the filter hole.

[0021] Furthermore, using a light plate as a contact plate makes it easier to illuminate the inside of the culture medium, thus facilitating the observation of the zebrafish's culture status.

[0022] Furthermore, the contact plate portion is positioned outside the opening slot to facilitate its removal.

[0023] Furthermore, setting multiple adjacent filter holes helps improve drainage efficiency, and sealing with the same sealing element helps save costs and reduce alignment difficulty.

[0024] The above description is only an overview of the technical solution of this utility model. In order to better understand the technical means of this utility model and to implement it in accordance with the contents of the specification, the preferred embodiments of this utility model are described in detail below with reference to the accompanying drawings. Attached Figure Description

[0025] Figure 1 This is a schematic diagram of the structure of a multi-well plate for zebrafish culture shown in Embodiment 1 of this utility model;

[0026] Figure 2 This is a cross-sectional view of a multi-well plate for zebrafish culture as shown in Embodiment 1 of this utility model.

[0027] Legend: 1-body, 11-culture well, 12-micropore, 13-filter well, 14-opening groove, 2-filling component, 21-sealing component, 22-closing plate, 23-contact plate, 231-light-emitting area, 232-power switch. Detailed Implementation

[0028] The technical solution of this utility model will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some, not all, of the embodiments of this utility model. Based on the embodiments of this utility model, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this utility model.

[0029] In the description of this utility model, it should be noted that the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," and "outer," etc., indicating the orientation or positional relationship, are based on the orientation or positional relationship shown in the accompanying drawings and are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0030] In the description of this utility model, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," and "joining" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0031] Furthermore, the technical features involved in the different embodiments of this utility model described below can be combined with each other as long as they do not conflict with each other.

[0032] Please see Figure 1 The multi-well plate for zebrafish culture protected by this utility model application includes several groups of culture wells 11, and each group of culture wells 11 includes multiple wells. The culture wells 11 in the same group and adjacent wells 11 are connected by micropores 12. The diameter of the micropores 12 is matched with the diameter of the zebrafish eggs. When the amount of culture medium added to the culture well 11 is a preset volume, the culture medium just enters the micropores 12.

[0033] By setting up microwells 12, there is no need to first remove the old culture medium and then quantitatively add the new culture medium. Instead, new culture medium is added on top of the old culture medium until it enters the microwells 12. At this point, the volume of the culture medium in the culture wells 11 is close to the preset volume, and the excess can be automatically transferred to the adjacent culture wells 11 without manual removal. Fish eggs with larger diameters cannot pass through the microwells 12. This structure reduces the difficulty of exposing fish eggs for culture.

[0034] Please refer to the following examples for details.

[0035] Example 1:

[0036] Please see Figure 1 The multi-well plate for zebrafish culture shown in a preferred embodiment of this application includes a body 1 and a filling component 2 that fits into the body 1.

[0037] The main body 1 is a colorless and transparent rectangle with six identical cylindrical culture wells 11 uniformly formed on its upper surface, arranged in two rows and three columns, with three culture wells 11 in each row forming a group. Two adjacent culture wells 11 in the same group are connected by circular micropores 12 on the sidewall. The three micropores 12 are arranged vertically, and the height of each micropore 12 is matched to the volume of commonly used culture medium, so that when the amount of culture medium in the culture well 11 reaches the preset volume, the liquid level of the culture medium is just tangent to the micropore 12. In this embodiment, the zebrafish need to be cultured in isolation; therefore, in each group of culture wells 11, culture is only carried out in the culture wells on both sides. When the liquid level reaches the height of the micropore 12, the culture medium flows through the micropore 12 to the middle culture well 11, thus ensuring that the amount of culture medium is the preset volume. In other embodiments, the culture media of each group of zebrafish can be interconnected. In this case, all culture wells 11 are used for culture, and when culture medium is observed entering the micropore 12, it can be determined that the amount of culture medium is the preset amount. In this embodiment, the diameter of the micropore 12 is 0.5 mm, which is smaller than the diameter of the fish egg, preventing the fish egg from entering the micropore 12. When the preset volume fits the upper micropore 12, the lower micropore 12 needs to be plugged with a soft plug. The soft plug is made of colorless and transparent flexible silicone material, with a small diameter through-hole and a large diameter sealing part. The diameter of the sealing part is slightly larger than that of the micropore 12, which facilitates the soft plug sealing the micropore 12.

[0038] The main body 1 also includes a rectangular opening groove 14. The opening groove 14 is located below each culture well 11 and forms an opening only on the side of the main body 1. The bottom surface of each culture well 11 is connected to the opening groove 14 through five vertical filter holes 13. The filter holes 13 have a diameter of 0.5 mm, one of which is coaxial with the culture well 11, and the other four are surrounding the central filter hole 13 and are arranged adjacent to it. In this embodiment, a different culture medium than the culture medium being transferred is required for cultivation. First, the previous culture medium flows through the filter hole 13 into the opening groove 14, separating it from the culture well 11. Then, the filter hole 13 is closed by the filling component 2, and new culture medium is added for cultivation.

[0039] Please see Figure 1 and Figure 2The filling component 2 includes sealing elements 21, a closing plate 22, and an abutment plate 23. In this embodiment, the closing plate 22 is a smooth, colorless, transparent rectangular plate structure with the same length and width as the opening groove 14, but lower in height. Six sealing elements 21, corresponding to the filter holes 13 in each culture well 11, are connected to one side surface of the opening groove 14. The sealing elements 21 are cylindrical, colorless, transparent, and flexible silicone structures. When the closing plate 22 is embedded in the opening groove 14, each sealing element 21 is coaxial with each culture well 11, and its top surface away from the closing plate 22 abuts against all the filter holes 13 in each culture well 11, thereby sealing the filter holes 13. In this embodiment, the sealing elements 21 are detachably connected to the closing plate 22 by adhesive, and connection marks are formed at the positions where the closing plate 22 connects to the sealing elements 21, facilitating replacement after the sealing elements 21 age or deform. The contact plate 23 has a rectangular plate structure, and its total height, together with the sealing member 21 and the closing plate 22, is equal to the height of the opening groove 14. It is used to fill the gap between the side of the closing plate 22 away from the sealing member 21 and the inner wall of the opening groove 14, so that the closing plate 22 can be embedded in the opening groove 14, and so that each sealing member 21 can be aligned with the filter hole 13, and ensure that the sealing member 21 is in close contact with each filter hole 13.

[0040] In this embodiment, the contact plate 23 is a waterproof LED light plate. Its length in the depth direction of the opening slot 14 is greater than that of the opening slot 14. Even when fully embedded in the opening slot 14, a portion of it remains outside the opening slot 14, facilitating the removal of the opening slot 14. The contact plate 23 has a light-emitting area 21 capable of controllable light emission and a power switch 232 indicating that the light-emitting area 21 is emitting light. The battery is built into the contact plate 23. The light-emitting area 21 is rectangular in shape, completely covering the projection of each culture well 11 onto the surface of the contact plate 23, facilitating observation of the culture conditions within each culture well 11. The power switch 232 is located on the side of the contact plate 23 away from the main body 1. In other embodiments, multiple light-emitting areas 21 can be provided, each covering the projection of each culture well 11 onto the surface of the contact plate 23, and multiple power switches 232 corresponding to the multiple light-emitting areas 21 can be provided, reducing the time of strong light irradiation on each culture well 11 during observation.

[0041] In this embodiment, the main body 1 and the sealing component 21 are disposable consumables, while the closing plate 22 and the contact plate 23 can be washed and reused.

[0042] By improving the structure of the multi-well plate, the steps for zebrafish exposure culture using multi-well plates are simplified, the operational difficulty is reduced, and the intuitiveness of observing the culture is improved, which helps to improve experimental efficiency and reduce the difficulty of observation.

[0043] Example 2:

[0044] The only difference between this embodiment and Embodiment 1 is that in this embodiment, each culture well 11 is provided with only one filter well 13, and the bottom surface of the culture well 11 is constructed as a cone coaxial with the filter well 13, with an angle of 3° between it and the horizontal plane.

[0045] Example 3:

[0046] The only difference between this embodiment and Embodiment 1 is that in this embodiment, the multiple filter holes 13 in each culture well 11 are dispersed and each corresponds to a sealing member 21.

[0047] Example 4:

[0048] The only difference between this embodiment and Embodiment 1 is that the diameter of the middle culture well 11 in this embodiment is smaller.

[0049] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0050] The embodiments described above are merely illustrative of several implementations of this utility model, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the utility model patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of this utility model, and these all fall within the protection scope of this utility model. Therefore, the protection scope of this utility model patent should be determined by the appended claims.

Claims

1. A multi-well plate for zebrafish culture, characterized by, The culture plate comprises a plurality of culture wells (11), and each group of the culture wells (11) comprises a plurality of culture wells (11) in the same group and adjacent to each other, and the culture wells (11) in the same group are communicated through micro-holes (12) with a diameter matched with the diameter of fish eggs of zebra fish, and when the amount of culture medium added in the culture wells (11) is a preset volume, the liquid surface of the culture medium is tangent to the micro-holes (12).

2. The multi-well plate for zebrafish culture of claim 1, wherein, A plurality of micro-holes (12) with different heights are arranged, the height of each micro-hole (12) is matched with a different preset volume, and each micro-hole (12) is controllably opened or closed.

3. The multi-well plate for zebrafish culture of claim 2, wherein, The micro-holes (12) are closed by soft plugs matched with the micro-holes (12), the soft plugs have a through part with a small diameter and a plug part with a large diameter matched with the micro-holes (12).

4. The multi-well plate for zebrafish culture of claim 1, wherein, The bottom of the culture well (11) is provided with a plurality of filter holes (13) for communicating the inside and outside of the culture well (11), the filter holes (13) have a diameter matched with the diameter of the fish eggs, and the filter holes (13) are controllably opened or closed.

5. The multi-well plate for zebrafish culture of claim 4, wherein, The culture plate comprises a body (1) and a filling assembly (2), the culture wells (11) are formed in the body (1), and the body (1) further comprises opening grooves (14) arranged below each culture well (11), the opening grooves (14) have openings arranged on the side surface of the body (1), and the top part is communicated with the inside of the culture well (11) through the filter holes (13), the filling assembly (2) is matched with the opening grooves (14) and is controllably filled in or separated from the opening grooves (14), and the filling assembly (2) is provided with a plurality of flexible sealing members (21) arranged on the side surface close to the filter holes (13) for opening or closing the filter holes (13).

6. The multi-well plate for zebrafish culture of claim 5, wherein, The filling assembly (2) comprises the sealing members (21), a closing plate (22) with a smooth surface, and a contact plate (23), the sealing members (21) are connected to the surface of the closing plate (22), the total thickness of the sealing members (21), the closing plate (22) and the contact plate (23) is matched with the height of the opening grooves (14), when the filling assembly (2) is filled in the opening grooves (14), the contact plate (23) is in contact with the inner wall of the opening grooves (14) and the side surface of the closing plate (22) away from the sealing members (21), and the sealing members (21) are in contact with the filter holes (13).

7. The multi-well plate for zebrafish culture of claim 6, wherein, The body (1), the closing plate (22) and the sealing members (21) are all configured as transparent structures, and the contact plate (23) is a light plate controllably emitting light towards the culture wells (11).

8. The multi-well plate for zebrafish culture of claim 6, wherein, When the contact plate (23) is filled in the opening grooves (14), part of the contact plate (23) is located outside the opening grooves (14).

9. The multi-well plate for zebrafish culture of claim 6, wherein, The plurality of filter holes (13) in the same culture well (11) are closed by the same sealing member (21).

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