Cell thawing and resuscitation instrument

Abstract

The utility model belongs to the technical field of biological experiment appliances, and particularly relates to a cell thawing and resuscitation instrument which comprises a barrel body, a motor is fixedly mounted on the upper surface of the barrel body, a rotating shaft is fixedly connected to the output end of the motor, a shaking device is connected to the outer side of the rotating shaft in a sleeved mode, and a mounting disc is arranged in the shaking device. A clamping disc is fixedly mounted on the upper surface of the mounting disc, a guide column is slidably connected to the clamping disc, an adjusting handle is fixedly mounted at one end of the guide column, a clamping block is fixedly mounted at the other end of the guide column, and the clamping disc, a sliding groove, the guide column, a first spring, a disc, the clamping block and other assemblies are used in cooperation; according to the device, the clamping blocks can be freely adjusted to clamp the cryopreservation tube main bodies, the adaptability to the cryopreservation tube main bodies of different specifications and materials is effectively improved, simultaneous thawing of the multiple cryopreservation tube main bodies is effectively achieved, operation is convenient and fast, batch cell thawing is better facilitated, and the universality and the application range of the device are improved.

Description

Technical Field

[0001] This utility model belongs to the field of biological experimental equipment technology, specifically a cell thawing and resuscitation device. Background Technology

[0002] With the rapid development of life sciences, significant progress has been made in fields such as cell therapy and cell culture. In cell therapy, such as CAR-T cell therapy for cancer treatment, a large number of high-quality cells are required. These cells are usually cryopreserved under low-temperature conditions and need to be thawed and revived before use. Similarly, in cell culture for drug development and toxicology testing, cells need to be obtained from cryopreserved cell banks and thawed.

[0003] When thawing, existing thawing and resuscitation instruments usually require placing cryovials containing frozen cells inside the instrument. However, existing devices are not convenient for clamping and thawing cryovials of different sizes, which can easily lead to uneven thawing. This has certain limitations, reduces thawing efficiency, and thus reduces practicality.

[0004] Therefore, this utility model provides a cell thawing and resuscitation device. Utility Model Content

[0005] In order to overcome the shortcomings of the prior art, at least one technical problem raised in the background art is solved.

[0006] The technical solution adopted by this utility model to solve its technical problem is as follows: The cell thawing and resuscitation instrument of this utility model includes a barrel body, a motor fixedly installed on the upper surface of the barrel body, a rotating shaft fixedly connected to the output end of the motor, a shaking device sleeved on the outer side of the rotating shaft, an installation plate inside the shaking device, a clamping plate fixedly installed on the upper surface of the installation plate, a guide post slidably connected to the clamping plate, an adjusting handle fixedly installed at one end of the guide post, a clamping block fixedly installed at the other end of the guide post, a cryopreservation tube body provided at one end of the clamping block, a fixing plate provided on one side of the clamping block, a second spring fixedly connected to one end face of the fixing plate, and the inner wall of the clamping plate fixedly connected to the other end of the second spring.

[0007] A movable mounting plate is provided on the outside of the barrel. A water tank is fixedly installed on the upper surface of the movable mounting plate. Multiple heating tubes are provided inside the water tank. A water pump is provided on the upper surface of the water tank. A delivery pipe is provided inside the water pump. A return pump is fixedly installed on the lower surface of the movable mounting plate. A delivery pipe is provided inside the return pump.

[0008] Preferably, the shaking device includes a socket plate, which is fitted onto the outside of the rotating shaft. The socket plate has multiple sets of springs, one end of which is fixedly connected to a mounting plate.

[0009] Preferably, a spring is provided on the outer side of the guide post, and a sliding groove is provided inside the clamping disk. The spring is located inside the sliding groove, one end of the spring is fixedly connected to one end of the disk, and the other end of the spring is fixedly connected to the inner wall of the sliding groove.

[0010] Preferably, a sleeve block is fitted onto the bottom end of the rotating shaft, and multiple sets of fan blades are fixedly installed on the outer side of the sleeve block.

[0011] Preferably, one end of each of the first and second conveying pipes is connected to a water tank, and the other end of each of the first and second conveying pipes is connected to a barrel.

[0012] Preferably, a temperature sensor is provided at the upper end of the water tank, and an indicator panel is installed on one side of the water tank. A first control button and a second control button are respectively provided on one side of the indicator panel, and the indicator panel and the temperature sensor are connected by a signal.

[0013] The beneficial effects of this utility model are as follows:

[0014] 1. The cell thawing and resuscitation device of this utility model uses components such as a clamping plate, a sliding groove, a guide column, a spring, a disc, and a clamping block in combination. The clamping block can be freely adjusted to clamp the cryopreservation tube body, effectively improving the adaptability to cryopreservation tube bodies of different specifications and materials, and effectively realizing the simultaneous thawing of multiple cryopreservation tube bodies. This makes the operation convenient, more conducive to the batch thawing of cells, and improves the versatility and applicability of the device.

[0015] 2. The cell thawing and resuscitation device described in this utility model uses components such as a water tank, a water pump, a return pump, a first delivery pipe, a second delivery pipe, a heating pipe, and an isothermal sensor to keep the sterile water in motion, resulting in a uniform water temperature distribution, improving the consistency of the resuscitation temperature, and enhancing cell activity.

[0016] 3. The cell thawing and resuscitation device of this utility model drives the rotating shaft to rotate through the output end of the motor. The rotating shaft drives the first sleeve plate to rotate. The first sleeve plate drives multiple sets of clamping devices to shake through the third spring. At the same time, the rotating shaft drives the bottom sleeve block to rotate. The sleeve block drives the fan blade to make a circular motion. The fan blade stirs the sterile water inside the barrel, thereby achieving up and down floating. This stirs and mixes the frozen cells inside the cryopreservation tube body, accelerating the cell resuscitation efficiency. Attached Figure Description

[0017] The present invention will be further described below with reference to the accompanying drawings.

[0018] Figure 1 This is a three-dimensional view of the overall structure of this utility model;

[0019] Figure 2 This is a cross-sectional view of the overall structure of this utility model;

[0020] Figure 3 This is a schematic diagram of the hydrothermal device and temperature control device of this utility model;

[0021] Figure 4 This is a cross-sectional view of the clamping device and the shaking device of this utility model;

[0022] Figure 5 This is a schematic diagram of the overall structure of the clamping device of this utility model;

[0023] In the diagram: 1. Barrel body; 2. Motor; 3. Rotating shaft; 4. Shaking device; 41. Connecting plate one; 42. Spring three; 5. Sleeve block; 6. Fan blade; 7. Freezing tube body; 8. Clamping plate; 9. Slide groove; 10. Guide column; 11. Spring one; 12. Disc; 13. Clamping block; 14. Spring two; 15. Fixing plate; 16. Adjusting handle; 17. Water tank; 18. Water pump; 19. Return pump; 20. Delivery pipe one; 21. Delivery pipe two; 22. Heating tube; 23. Temperature sensor; 24. Indicator panel; 25. First control button; 26. Second control button; 27. Movable mounting plate; 28. Mounting plate. Detailed Implementation

[0024] To make the technical means, creative features, objectives and effects of this utility model easier to understand, the present utility model will be further described below in conjunction with specific embodiments.

[0025] like Figures 1 to 5As shown in the embodiment of this utility model, a cell thawing and resuscitation device includes a barrel 1. A motor 2 is fixedly installed on the upper surface of the barrel 1. A rotating shaft 3 is fixedly connected to the output end of the motor 2. A sleeve block 5 is sleeved on the bottom end of the rotating shaft 3. Multiple sets of fan blades 6 are fixedly installed on the outer side of the sleeve block 5. A shaking device 4 is sleeved on the outer side of the rotating shaft 3. The shaking device 4 includes a first sleeve plate 41, which is sleeved on the outer side of the rotating shaft 3. Multiple sets of third springs 42 are arranged inside the first sleeve plate 41. One end of each set of third springs 42 is fixedly connected to a mounting plate 28. A clamping plate 8 is fixedly installed on the upper surface of the mounting plate 28. The clamping plate 8 is slidably connected to... There is a guide post 10, and a spring 11 is provided on the outer side of the guide post 10. A sliding groove 9 is opened inside the clamping plate 8. The spring 11 is located inside the sliding groove 9. One end of the spring 11 is fixedly connected to one end of the disc 12, and the other end of the spring 11 is fixedly connected to the inner wall of the sliding groove 9. An adjustment handle 16 is fixedly installed on one end of the guide post 10, and a clamping block 13 is fixedly installed on the other end of the guide post 10. A cryopreservation tube body 7 is provided on one end of the clamping block 13. A fixing plate 15 is provided on one side of the clamping block 13. A spring 2 14 is fixedly connected to one end of the fixing plate 15, and the other end of the spring 2 14 is fixedly connected to the inner wall of the clamping plate 8.

[0026] A movable mounting plate 27 is provided on the outside of the tank body 1. A water tank 17 is fixedly installed on the upper surface of the movable mounting plate 27. Multiple heating tubes 22 are provided inside the water tank 17. A water pump 18 is provided on the upper surface of the water tank 17. A first delivery pipe 20 is provided inside the water pump 18. A return pump 19 is fixedly installed on the lower surface of the movable mounting plate 27. A second delivery pipe 21 is provided inside the return pump 19. A temperature sensor 23 is provided at the upper end of the water tank 17. An indicator panel 24 is installed on one side of the water tank 17. A first control button 25 and a second control button 26 are respectively provided on one side of the indicator panel 24. The indicator panel 24 and the temperature sensor 23 are connected by a signal.

[0027] Specifically, a movable mounting plate 27 is fixedly installed on the outer side of the tank body 1. A water pump 18 is fixedly installed on the upper surface of the movable mounting plate 27. A first delivery pipe 20 is provided inside the water pump 18. One end of the first delivery pipe 20 is connected to the inside of the water tank 17, and the other end is connected to the inside of the tank body 1. A return pump 19 is fixedly installed on the lower surface of the movable mounting plate 27. A second delivery pipe 21 is provided inside the return pump 19. One end of the second delivery pipe 21 is connected to the inside of the water tank 17, and the other end is connected to the inside of the tank body 1. A temperature sensor 23 is movably installed on the upper end of the water tank 17. An indicator panel 24, a first control button 25, and a second control button 26 are sequentially installed on one side of the water tank 17. The water tank 17 has multiple heating tubes 22 installed inside. The temperature sensor 23 is connected to the indicator panel 24. The first control button 25 controls the heating tubes 22. A motor 2 is fixedly installed on the upper surface of the tank 1. The output shaft of the motor 2 is fixedly connected to a rotating shaft 3. A sleeve block 5 is sleeved at the bottom end of the rotating shaft 3. Multiple fan blades 6 are movably installed on the outside of the sleeve block 5. A connecting plate 41 is installed at the upper end of the multiple fan blades 6. The connecting plate 41 is fixedly installed on the outside of the rotating shaft 3. One end of multiple springs 42 is fixedly connected inside the connecting plate 41. The other end of the multiple springs 42 is fixedly connected to a mounting plate 28. Multiple clamping plates 8 are fixedly installed on the upper surface of the mounting plate 28. Guide posts 10 are slidably connected inside each of the multiple clamping plates 8. One end of each guide post 10 is fixedly equipped with a clamping block 13, and the other end of each guide post 10 is fixedly connected to an adjusting handle 16. The clamping block 13 is made of flexible material. One end of each clamping block 13 contacts the cryopreservation tube body 7, and the other end of each clamping block 13 slides in contact with a fixing plate 15. One end of each fixing plate 15 is fixedly connected to a spring 42, and the other end of each spring 42 is fixedly connected to the inner wall of the clamping plate 8. A spring 11 is provided on the outer side of the guide post 10, and a sliding groove 9 is provided inside the clamping plate 8. The spring 11 is located inside the sliding groove 9 at the beginning of the clamping plate 8. One end of each spring 11 is fixedly connected to one end of a disc 12, and the other end of each spring 11 is fixedly connected to the inner wall of the sliding groove 9. The disc 12 is sleeved on the guide post 10. On the outside, the operator first places the cells to be thawed into the cryovial body 7. The operator then pulls the adjusting handle 16, which causes the guide column 10 to reciprocate horizontally within the clamping plate 8. The guide column 10 moves the disc 12, which in turn moves the spring 11. The spring 11 deforms under tension, causing the guide column 10 to move the clamping block 13, which is fixed at one end, to reciprocate horizontally. During this movement, the clamping block 13 slides into contact with the fixing plate 15. The fixing plate 15 is compressed, causing the spring 14 to deform under pressure. This allows the fixing plate 15 to limit the position of the clamping block 13. After placing the cryovial body 7 on one side of the clamping block 13, the operator pushes back the guide column 10.The elastic force generated by the tension of spring 11 drives the guide post 10 and clamping block 13 through the disc 12 to quickly and smoothly clamp the outside of the cryopreservation tube body 7. The multiple clamping devices effectively increase the number of cryopreservation tube bodies 7 that can be thawed at once, enabling simultaneous thawing of multiple cryopreservation tube bodies 7, making operation convenient and facilitating batch thawing of cells. The heating tube 22 inside the water tank 17 heats the sterile water inside. The first control button 25 controls the heating tube 22. The temperature sensor 23 detects the temperature of the sterile water inside the water tank 17 and transmits the signal to the indicator panel 24. When the sterile water reaches the appropriate temperature, the operator starts the water pump 18 and the return pump 19 respectively. The water pump 18 and the return pump 19 are existing devices; reference can be made to the R300-1000PC water pump 18 and the CY-DCY-12YL return pump 19. The working principle of pump 19 is existing technology and will not be elaborated here. Pump 18 and pump 19 circulate sterile water into the interior of tank 1 through delivery pipe 20 and delivery pipe 21, respectively. Simultaneously, they work with heating pipe 22 to keep the sterile water in motion, ensuring uniform water temperature distribution, improving the consistency of resuscitation temperature, and enhancing cell activity. Next, the operator starts motor 2. Motor 2 is an existing device; refer to Y-series motor 2. The working principle of Y-series motor 2 is existing technology and will not be elaborated here. The output of motor 2 drives shaft 3 to rotate, which in turn drives socket 41 to rotate. Socket 41, through spring 42, causes multiple clamping devices to sway. Simultaneously, shaft 3 drives bottom sleeve 5 to rotate, which in turn drives fan blades 6 in a circular motion. Fan blades 6 stir the sterile water inside tank 1, causing it to float up and down, thus mixing the frozen cells inside cryopreservation tube 7 and accelerating cell resuscitation efficiency.

[0028] like Figure 5 As shown, a spring 11 is provided on the outer side of the guide post 10, and a groove 9 is provided inside the clamping disk 8. The spring 11 is located inside the groove 9. One end of the spring 11 is fixedly connected to one end of the disk 12, and the other end of the spring 11 is fixedly connected to the inner wall of the groove 9.

[0029] Specifically, the operator pulls the adjustment handle 16, which causes the guide column 10 to reciprocate horizontally inside the clamping plate 8. The guide column 10 drives the disc 12 to move, and the disc 12 drives the spring 11 to move. The spring 11 deforms under tension, and the guide column 10 drives the clamping block 13, which is fixed at one end, to reciprocate horizontally. When the clamping block 13 moves, it slides into contact with the fixing plate 15. The fixing plate 15 is compressed, which drives the spring 14. The spring 14 deforms under the compression force, so the position of the clamping block 13 can be limited by the fixing plate 15. After the operator places the cryopreservation tube body 7 on one side of the clamping block 13, he pushes the guide column 10 back. The elastic force generated by the tension of the spring 11 drives the guide column 10 and the clamping block 13 through the disc 12 to quickly and smoothly clamp the outside of the cryopreservation tube body 7.

[0030] like Figures 1 to 2 As shown, one end of the first conveying pipe 20 and the second conveying pipe 21 are respectively connected to a water tank 17, and the other end of the first conveying pipe 20 and the second conveying pipe 21 are respectively connected to a barrel body 1.

[0031] Specifically, the pump 18 and the return pump 19 circulate sterile water into the interior of the tank 1 through the first delivery pipe 20 and the second delivery pipe 21, respectively. At the same time, they work in conjunction with the heating pipe 22 to keep the sterile water in motion, so that the overall water temperature is evenly distributed, improving the consistency of the resuscitation temperature and enhancing cell activity.

[0032] like Figure 3 As shown, a temperature sensor 23 is installed at the upper end of the water tank 17, and an indicator panel 24 is installed on one side of the water tank 17. A first control button 25 and a second control button 26 are respectively installed on one side of the indicator panel 24. The indicator panel 24 and the temperature sensor 23 are connected by a signal.

[0033] Specifically, the heating element 22 inside the water tank 17 heats the sterile water inside, the first control button 25 controls the heating element 22, and the temperature sensor 23 detects the temperature of the sterile water inside the water tank 17 and transmits the signal to the indicator panel 24. Thus, the temperature of the sterile water can be controlled through the indicator panel 24 and the first control button 25.

[0034] Working principle: First, the operator places the cells to be thawed into the cryovial body 7. The operator then pulls the adjustment handle 16, which causes the guide column 10 to reciprocate horizontally within the clamping plate 8. The guide column 10 moves the disc 12, which in turn moves the spring 11. The spring 11 deforms under tension, causing the clamping block 13, which is fixed at one end, to reciprocate horizontally. During this movement, the clamping block 13 slides against the fixing plate 15, causing the fixing plate 15 to be compressed and thus driving the spring 14 to deform under pressure. This allows the fixing plate 15 to limit the position of the clamping block 13. After the operator places the cryopreservation tube body 7 on one side of the clamping block 13, they push back the guide post 10. The elastic force generated by the tension of the spring 11 drives the guide post 10 and the clamping block 13 through the disc 12 to quickly and smoothly clamp the outside of the cryopreservation tube body 7. The multiple clamping devices effectively increase the number of cryopreservation tube bodies 7 that can be thawed at one time, thus enabling the simultaneous thawing of multiple cryopreservation tube bodies 7, making the operation more convenient and facilitating batch thawing of cells. The heating tube 22 inside the water tank 17 heats the sterile water inside. The first control button 25 controls the heating tube 22. The temperature sensor 23 detects the temperature of the sterile water inside the water tank 17 and transmits the signal. On the indicator panel 24, after the sterile water is heated to a suitable temperature, the operator starts the water pump 18 and the return pump 19 respectively. The water pump 18 and the return pump 19 are existing devices, and can be referenced to the R300-1000PC water pump 18 and the CY-DCY-12YL return pump 19. The working principles of the R300-1000PC water pump 18 and the CY-DCY-12YL return pump 19 are existing technologies and will not be elaborated upon here. The water pump 18 and the return pump 19 circulate the sterile water into the interior of the tank 1 through the first delivery pipe 20 and the second delivery pipe 21 respectively. Simultaneously, they work in conjunction with the heating pipe 22, thus keeping the sterile water in a state of motion, ensuring the entire system... The uniform distribution of body water temperature improves the consistency of resuscitation temperature and enhances cell activity. Next, the operator starts motor 2. Motor 2 is an existing device; refer to Y-series motor 2. The working principle of Y-series motor 2 is existing technology and will not be elaborated here. The output end of motor 2 drives the rotating shaft 3 to rotate, which in turn drives the connecting plate 41 to rotate. The connecting plate 41, through spring 42, drives multiple sets of clamping devices to sway. Simultaneously, the rotating shaft 3 drives the bottom sleeve block 5 to rotate, which in turn drives the fan blade 6 to perform circular motion. The fan blade 6 stirs the sterile water inside the tank 1, thus achieving up-and-down floating, stirring and mixing the frozen cells inside the cryopreservation tube body 7, accelerating cell resuscitation efficiency.

[0035] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claimed utility model. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A cell thawing and recovery instrument, characterized by: The application relates to a freezing storage device, which comprises a barrel body (1), a motor (2) fixedly arranged on the upper surface of the barrel body (1), a rotating shaft (3) fixedly connected to the output end of the motor (2), a shaking device (4) sleeved on the outer side of the rotating shaft (3), an installation disc (28) arranged in the shaking device (4), a clamping disc (8) fixedly arranged on the upper surface of the installation disc (28), a guide column (10) slidingly connected to the clamping disc (8), an adjusting handle (16) fixedly arranged on one end of the guide column (10), a clamping block (13) fixedly arranged on the other end of the guide column (10), a freezing storage tube main body (7) arranged on one end of the clamping block (13), a fixed plate (15) arranged on one side of the clamping block (13), a spring No. 2 (14) fixedly connected to one end of the fixed plate (15), and an inner wall of the clamping disc (8) fixedly connected to the other end of the spring No. 2 (14). An activity mounting plate (27) is arranged on the outer side of the barrel body (1), a water tank (17) is fixedly arranged on the upper surface of the activity mounting plate (27), a plurality of groups of heating pipes (22) are arranged in the water tank (17), a water pump (18) is arranged on the upper surface of the water tank (17), a conveying pipe No. 1 (20) is arranged in the water pump (18), and a back-pumping pump (19) is fixedly arranged on the lower surface of the activity mounting plate (27).

2. A cell thawing and recovery apparatus as claimed in claim 1, wherein: The shaking device (4) comprises a sleeving disc No. 1 (41) sleeved on the outer side of the rotating shaft (3), and a plurality of groups of springs No. 3 (42) are arranged in the sleeving disc No. 1 (41).

3. A cell thawing and recovery apparatus as claimed in claim 2, wherein: A spring No. 1 (11) is arranged on the outer side of the guide column (10), a sliding groove (9) is formed in the clamping disc (8), the spring No. 1 (11) is located in the sliding groove (9), one end of the spring No. 1 (11) is fixedly connected to one end of a disc (12), and the other end of the spring No. 1 (11) is fixedly connected to the inner wall of the sliding groove (9).

4. A cell thawing and recovery apparatus as claimed in claim 3, wherein: A sleeve block (5) is sleeved on the bottom end of the rotating shaft (3), and a plurality of groups of fan blades (6) are fixedly arranged on the outer side of the sleeve block (5).

5. A cell thawing and recovery apparatus as claimed in claim 4, wherein: One end of the conveying pipe No. 1 (20) and the conveying pipe No. 2 (21) is respectively connected to the water tank (17), and the other end of the conveying pipe No. 1 (20) and the conveying pipe No. 2 (21) is respectively connected to the barrel body (1).

6. A cell thawing and recovery apparatus as claimed in claim 5, wherein: A temperature sensor (23) is arranged on the upper end of the water tank (17), an indicating panel (24) is arranged on one side of the water tank (17), a first control button (25) and a second control button (26) are respectively arranged on one side of the indicating panel (24), and the indicating panel (24) and the temperature sensor (23) are signal-connected.

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