Temperature-controlled three-dimensional fluorescent drug sensitivity detection device
By integrating a temperature-controlled three-dimensional fluorescence drug susceptibility testing device, the problems of long time and inaccurate results in traditional drug susceptibility testing methods are solved, and automated, rapid and accurate construction and detection of drug concentration gradients are achieved.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-16
- Publication Date
- 2026-04-03
AI Technical Summary
Traditional drug sensitivity testing methods rely on pure microbial culture and manual interpretation, which results in long testing times, inaccurate results, and poor reproducibility, making it difficult to meet the rapid testing needs of emergency diagnosis and treatment.
The device employs a temperature-controlled three-dimensional fluorescence drug sensitivity detection system, which integrates a retractable culture dish support, a temperature control mechanism, a fluorescence imaging system, and a drug delivery system to achieve automated temperature control, drug delivery, and fluorescence monitoring, thereby reducing human error.
It improves the efficiency and accuracy of drug susceptibility testing, reduces the risk of contamination, and enables rapid and accurate construction of drug concentration gradients and result analysis.
Smart Images
Figure CN224077394U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to the field of drug sensitivity detection technology, specifically a temperature-controlled three-dimensional fluorescence drug sensitivity detection device. Background Technology
[0002] Traditional antimicrobial susceptibility testing methods, such as disk diffusion, microdilution, and gradient diffusion, suffer from significant time limitations due to their high dependence on pure microbial culture and manual interpretation. For example, the disk diffusion method requires placing drug-containing disks on agar plates inoculated with microorganisms and waiting 18 to 24 hours until a visible inhibition zone appears. This process is limited by the inherent growth cycle of microorganisms and requires manual visual inspection or measurement of the inhibition zone diameter using calipers, making it susceptible to errors in visual interpretation and thus affecting the accuracy of the test results.
[0003] The core problems with the aforementioned drug susceptibility testing technologies lie in the limitations of the microbial growth cycle and the low degree of automation in the auxiliary interpretation process. The microdilution method requires manual preparation of different drug concentrations on a 96-well plate, relying on turbidimetry to determine microbial growth inhibition. However, this method has poor sensitivity for low-concentration bacterial solutions and requires additional time to increase signal intensity. Meanwhile, although the gradient diffusion method combines the principles of diffusion and dilution, its results still depend on the uniformity of physical contact between the test strip and the agar medium, making it susceptible to temperature fluctuations or changes in culture medium composition, resulting in poor reproducibility of the test results.
[0004] Therefore, we propose a temperature-controlled three-dimensional fluorescence drug sensitivity detection device to address the problems mentioned above. Utility Model Content
[0005] This invention provides a temperature-controlled three-dimensional fluorescence drug susceptibility testing device, which can solve the main problem that traditional drug susceptibility testing methods, including the paper disc diffusion method, microdilution method, and gradient diffusion method, rely heavily on pure microbial culture and manual interpretation, are time-consuming, and cannot meet the rapid testing needs in emergency diagnosis and treatment.
[0006] To solve the above-mentioned technical problems, this utility model provides the following technical solution:
[0007] A temperature-controlled three-dimensional fluorescence drug sensitivity detection device includes an incubator with a retractable culture dish support inside and a temperature control mechanism installed inside. A fluorescence imaging system is installed above the retractable culture dish support. The fluorescence imaging system includes a multispectral camera and a UV-Vis dual-band LED array installed on the upper wall inside the incubator. A drug delivery system is installed on one side of the incubator, which includes a rotary multi-channel micro-injection pump, a precision needle connected to the injection pump, and a robotic arm carrying the precision needle. A temperature-controlled drug box is installed on the side wall of the incubator, and a drug storage turntable is installed inside the temperature-controlled drug box.
[0008] Compared with the prior art, the beneficial effects achieved by this utility model are:
[0009] This invention utilizes an incubator as its core, integrating a retractable petri dish support, a temperature control mechanism, a fluorescence imaging system, and a drug delivery system. The temperature control mechanism precisely regulates the incubator temperature based on real-time feedback from built-in distributed temperature sensors, providing an optimal environment for microbial growth. Simultaneously, the fluorescence imaging system employs a multispectral camera and a UV-Vis dual-band LED array to monitor the microbial growth status under drug influence in real time, reducing errors from manual observation. The drug delivery system includes a precision needle and a rotary multi-channel microinjection pump, enabling automated delivery of different drug concentrations, improving detection efficiency and accuracy. These improvements not only solve the problems of manual operation and contamination in traditional methods but also significantly enhance the accuracy and throughput of drug sensitivity testing through a fully enclosed automated structure. Attached Figure Description
[0010] Figure 1 This is a schematic diagram of the retractable culture dish support of this utility model in its open state.
[0011] Figure 2 This is a front sectional view of the incubator of this utility model;
[0012] Figure 3 This is a schematic diagram of the internal structure of the incubator of this utility model;
[0013] Figure 4 This is a schematic diagram of the internal structure of the temperature-controlled medicine box of this utility model.
[0014] The components include: 1. Incubator; 2. Retractable petri dish support; 5. Multispectral camera; 6. UV-Vis dual-band LED array; 9. Precision needle; 10. Robotic arm; 11. Temperature-controlled medicine box; 12. Medicine storage turntable; 13. Semiconductor cooling chip; 14. Heating resistance wire; 15. Protective partition; 17. Sliding seat; 18. Petri dish body; 19. Circulating conveyor belt; 20. Fixed seat; 21. Slide rail; 22. Slide groove; 23. Pick-up and drop-off port; 24. Sealing door panel; 25. Electric telescopic rod; 26. Connecting hose; 28. Medicine bottle; 29. Clean water tank; 30. Collection tank; 31. Wastewater tank. Detailed Implementation
[0015] The specific embodiments of this utility model are described in detail below, but it should be understood that the scope of protection of this utility model is not limited to the specific embodiments.
[0016] Example 1:
[0017] Please see Figure 1-4This utility model provides a technical solution:
[0018] A temperature-controlled three-dimensional fluorescence drug sensitivity detection device includes an incubator 1, an incubator 1 with a retractable culture dish support 2 inside, and a temperature control mechanism installed inside the incubator 1. A fluorescence imaging system is installed above the retractable culture dish support 2. The fluorescence imaging system includes a multispectral camera 5 and an ultraviolet-visible dual-band LED array 6 installed on the upper wall inside the incubator 1. A drug delivery system is installed on one side of the incubator 1, which includes a rotary multi-channel micro-injection pump, a precision needle 9 connected to the injection pump, and a robotic arm 10 carrying the precision needle 9. A temperature-controlled drug box 11 is installed on the side wall of the incubator 1, and a drug storage turntable 12 is installed inside the temperature-controlled drug box 11.
[0019] In the above scheme, the incubator 1 is the core, and it integrates a retractable culture dish support 2, a temperature control mechanism, a fluorescence imaging system, and a drug delivery system. The retractable culture dish support 2 can be adjusted in position by mechanical sliding to facilitate the entry and exit of the culture dish body 18 into and out of the incubator 1.
[0020] The temperature inside the incubator 1 is precisely controlled by a temperature control mechanism to simulate the optimal environment for microbial growth. The fluorescence imaging system consists of a multispectral camera 5 and a UV-Vis dual-band LED array 6. The LED array provides excitation light, and the multispectral camera 5 captures the three-dimensional fluorescence signals generated by microbial metabolism, enabling real-time monitoring of the microbial growth status under drug action.
[0021] Furthermore, the drug delivery system uses a rotary multi-channel micro-injection pump to draw drugs of different concentrations from the temperature-controlled drug tank 11, which are then precisely injected into the culture dish body 18 by a precision needle 9 mounted on the robotic arm 10, forming a concentration gradient. Inside the temperature-controlled drug tank 11, a drug storage turntable 12 holds pre-configured drug bottles 28 and a water tank 29 to ensure that the drugs are stored at a constant temperature and to prevent deterioration.
[0022] Through the above scheme, the upper and lower supports of the culture dish body 18 and the fluorescence imaging system work together to ensure that the culture dish body 18 is stably positioned during imaging, avoiding motion blur. The robotic arm 10 of the drug delivery system works in conjunction with the rotary injection pump to achieve automated delivery of drugs of multiple concentrations. The above scheme solves the problems of traditional drug sensitivity testing relying on manual operation, which is inefficient and prone to contamination. The fully automated structure of the temperature control mechanism, fluorescence imaging system and drug delivery system improves detection accuracy and avoids human error.
[0023] The temperature control mechanism includes a semiconductor cooling chip 13 and a heating resistance wire 14; the semiconductor cooling chip 13 and the heating resistance wire 14 are respectively fixedly installed inside the side wall of the incubator 1, and a protective partition 15 is provided between the semiconductor cooling chip 13 and the heating resistance wire 14; a distributed temperature sensor array is provided on the inner wall of the incubator 1.
[0024] In the above scheme, the semiconductor cooling chip 13 and the heating resistance wire 14 are responsible for cooling and heating respectively. Temperature data is fed back in real time through a distributed temperature sensor array, and the cooling or heating power is dynamically adjusted by the control module to maintain a constant temperature in the incubator 1.
[0025] The protective partition 15 isolates the cooling plate and heating wire to prevent direct collision of hot and cold air currents from interfering with temperature control efficiency; the distributed temperature sensor covers multiple points inside the incubator 1, and the multi-point detection ensures temperature uniformity and avoids local overheating or overcooling; it solves the problems of slow response and low accuracy of traditional temperature control systems, which affect the consistency of microbial growth; this design achieves rapid and stable temperature regulation through zoned temperature control.
[0026] The retractable petri dish support 2 includes a sliding base 17 and several petri dish bodies 18. A circulating conveyor belt 19 is provided on the upper part of the sliding base 17, and the petri dish bodies 18 are arranged on the upper part of the circulating conveyor belt 19. The circulating conveyor belt 19 drives the petri dish bodies 18 to rotate on the upper part of the sliding base 17. A fixed base 20 is provided inside the incubator 1, and the sliding base 17 is slidably connected to the upper part of the fixed base 20. A slide rail 21 is provided on the surface of the fixed base 20, and a sliding groove 22 matching the slide rail 21 is provided at the bottom of the sliding base 17.
[0027] A circulating conveyor belt 19 is installed on the upper part of the sliding seat 17, driving multiple culture dish bodies 18 to rotate in a cycle, facilitating sequential drug injection and fluorescence imaging. The sliding seat 17 engages with the slide rail 21 of the fixed seat 20 via a bottom groove 22, and is driven back and forth by an electric telescopic rod 25 to extend and retract the support of the culture dish bodies 18. The telescopic design of the sliding seat 17 facilitates the loading and unloading of batches of culture dish bodies 18, reducing the influence of the external environment on the internal temperature and humidity of the incubator 1. The circulating conveyor belt 19 allows multiple culture dish bodies 18 to be processed sequentially, increasing the detection throughput. This solves the problems of low efficiency and easy contamination associated with traditional manual replacement of culture dish bodies 18; this structure achieves fully enclosed automated operation, improving detection efficiency and safety.
[0028] The incubator 1 has an opening 23 at one end. A sealing door 24 is fixedly connected to the end of the sliding seat 17 near the opening 23. An electric telescopic rod 25 is fixedly connected to the fixed seat 20. The telescopic end of the electric telescopic rod 25 is fixedly connected to the inside of the sealing door 24. When the electric telescopic rod 25 pushes the sliding seat 17 to move, it drives the sealing door 24 fixed to the end of the sliding seat 17 to open and close synchronously, sealing the opening 23 of the incubator 1.
[0029] When it is necessary to insert or remove the petri dish body 18, the sliding seat 17 extends outward, and the sealing door 24 moves outward accordingly, exposing the insertion / removal port 23; after the operation is completed, it retracts and seals.
[0030] The sealed door 24 is tightly fitted to the loading / unloading port 23 to prevent external air from entering and interfering with the temperature and humidity inside the incubator 1. This avoids the temperature fluctuations and contamination risks caused by traditional open operation; this design achieves fully enclosed automation of the storage, retrieval, and detection process of the culture dish body 18.
[0031] A connecting hose 26 is provided between the precision needle 9 and the rotary multichannel microinjection pump. When using a single needle system, after injection, the robotic arm 10 moves the needle to the collection tank 30, and the rotary multichannel microinjection pump draws clean water from the clean water tank 29 to rinse the tubing and needle, and then blows away the residual liquid.
[0032] In the above solution, the clean water tank 29 is integrated with the drug storage turntable 12, and the cleaning process is automated by switching the position of the turntable. This avoids cross-contamination that can easily occur when using the same needle for multiple concentrations of drugs; this design ensures that there is no residue on the needle through the cleaning steps, guaranteeing the accuracy of the concentration.
[0033] A base is provided at one end of the robotic arm 10, and the base of the robotic arm 10 is fixedly connected to the side wall of the incubator 1. A precision needle 9 is installed at the end of the robotic arm 10 near the circulating conveyor belt 19, and the precision needle 9 is located on the upper part of the circulating conveyor belt 19. The reagent storage turntable 12 stores reagent bottles 28 of different concentrations of experimental drugs and a water tank 29. The reagent storage turntable 12 drives the reagent bottles 28 and the water tank 29 to rotate and switch positions. A rotary multi-channel micro-injection pump can pump experimental drugs or water of different concentrations.
[0034] The robotic arm 10 positions the precision needle 9 onto the target culture dish body 18 on the circulating conveyor belt according to the program. The rotary multi-channel micro-injection pump draws the specified concentration of drug from the drug storage turntable 12 and injects it precisely through the needle. The drug storage turntable 12 can rotate to switch the position of different concentration drug bottles 28 or water tanks 29 for the injection pump to draw as needed.
[0035] The system utilizes the flexible movement of the robotic arm 10 in conjunction with the cyclical rotation of the conveyor belt to continuously process the culture dish 18. This addresses the shortcomings of traditional drug gradient preparation methods, which rely on manual dilution, are time-consuming, and prone to errors. The system achieves rapid and precise concentration gradient construction through automated extraction and targeted injection.
[0036] A collection tank 30 is located below the robotic arm 10, and a wastewater tank 31 is located at the bottom of the temperature-controlled medicine tank 11. The bottom of the collection tank 30 is connected to the interior of the wastewater tank 31. A control module is located on the top of the temperature-controlled box, and the control module is electrically connected to a display screen.
[0037] Waste liquid generated from cleaning the needles flows into the collection tank 30 below the robotic arm 10, and then through pipes into the wastewater tank 31 at the bottom of the temperature-controlled medicine tank 11 for centralized treatment. The control module integrates commands for temperature control, movement of the robotic arm 10, and operation of the syringe pump, and enables human-machine interaction and real-time monitoring through a display screen. The waste liquid collection system prevents contamination of the internal environment of the incubator 1, maintaining the cleanliness of the test. This design solves the problem of interrupting the testing process for traditional waste liquid treatment; it achieves seamless integration of automatic waste liquid collection and continuous operation of the device.
[0038] The specific working principle of this temperature-controlled three-dimensional fluorescence drug sensitivity detection device is as follows:
[0039] After the device is started, the retractable culture dish support 2 drives the sliding seat 17 to extend outward along the slide rail 21 via the electric telescopic rod 25, which in turn opens the loading and unloading port 23 on the sealing door 24. After the operator places multiple culture dish bodies 18 on the circulating conveyor belt 19 on the upper part of the sliding seat 17, the sliding seat 17 retracts and seals the incubator 1. The temperature control mechanism dynamically adjusts the temperature in different zones based on real-time data from the distributed temperature sensor array, using the semiconductor cooling chip 13 and heating resistance wire 14. The protective partition 15 isolates hot and cold airflows, maintaining a constant microbial growth environment inside the chamber. Subsequently, the precision needle 9 mounted on the robotic arm 10 is positioned to the target culture dish 18 on the circulating conveyor belt under program control. A rotary multi-channel micro-injection pump draws a preset concentration of drug or water from the drug storage turntable 12 in the temperature-controlled drug tank 11, and injects it precisely into the culture dish 18 through the needle to form a discrete concentration gradient. If a single needle is used, after injection, the robotic arm 10 moves to the water tank 29, and the injection pump automatically draws water to rinse the pipeline and blows it dry. Waste liquid is collected in the collection tank 30 and discharged into the bottom wastewater tank 31 to avoid cross-contamination. The fluorescence imaging system works simultaneously: the ultraviolet-visible dual-band LED array 6 projects excitation light, and the multispectral camera 5 captures the three-dimensional fluorescence signal of microbial metabolism. Combined with the rotation of the circulating conveyor belt, all culture dishes 18 are scanned sequentially to analyze the morphology of the inhibition zone and the change in inhibition rate in real time. The entire process is controlled by a control module that integrates temperature control, robotic arm 10 movement, drug delivery, and imaging analysis. The detection results are output on the display screen. This device solves the problems of low efficiency, high risk of contamination, and insufficient temperature control accuracy of traditional manual operation by using closed-loop environmental control, automated drug delivery, and dynamic three-dimensional fluorescence monitoring, thus achieving efficient and accurate drug sensitivity detection and MIC determination.
[0040] The above-disclosed embodiments are only a few specific examples of the present utility model. However, the embodiments of the present utility model are not limited thereto. Any changes that can be conceived by those skilled in the art should fall within the protection scope of the present utility model.
Claims
1. A temperature-controlled three-dimensional fluorescence drug sensitivity detection device comprising a culture box (1), characterized in that: The inside of the incubator (1) is provided with a retractable culture dish support (2), and the inside of the incubator (1) is provided with a temperature control mechanism, the upper part of the retractable culture dish support (2) is provided with a fluorescence imaging system, the fluorescence imaging system includes a multispectral camera (5) installed on the upper wall of the incubator (1) and a ultraviolet-visible double-band LED array (6), one side of the incubator (1) is provided with a drug delivery system, which includes a rotary multi-channel micro-injection pump, a precision needle (9) communicated with the injection pump, and a mechanical arm (10) carrying the precision needle (9), a temperature control medicine box (11) is installed on the side wall of the incubator (1), and the inside of the temperature control medicine box (11) is provided with a medicine storage turntable (12).
2. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 1, characterized in that: The temperature control mechanism includes a semiconductor refrigerating fin (13) and a heating resistance wire (14); the semiconductor refrigerating fin (13) and the heating resistance wire (14) are fixedly installed in the inside of the side wall of the incubator (1) respectively, and a protective partition (15) is arranged between the semiconductor refrigerating fin (13) and the heating resistance wire (14); a distributed temperature sensor array is arranged on the inner wall of the incubator (1).
3. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 1, characterized in that: The retractable culture dish support (2) includes a sliding seat (17) and a plurality of culture dish bodies (18), the upper part of the sliding seat (17) is provided with a circulating conveyor belt (19), and the plurality of culture dish bodies (18) are arranged on the upper part of the circulating conveyor belt (19), the circulating conveyor belt (19) drives the culture dish bodies (18) to rotate on the upper part of the sliding seat (17).
4. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 1, characterized in that: The inside of the incubator (1) is provided with a fixed seat (20), and the sliding seat (17) is slidingly connected to the upper part of the fixed seat (20).
5. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 4, characterized in that: The surface of the fixed seat (20) is provided with a sliding rail (21), and the bottom of the sliding seat (17) is provided with a sliding groove (22) matched with the sliding rail (21).
6. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 1, characterized in that: One end of the incubator (1) is provided with a taking and placing opening (23), one end of the sliding seat (17) close to the taking and placing opening (23) is fixedly connected with a sealing door plate (24), the fixed seat (20) is fixedly connected with an electric telescopic rod (25), and the telescopic end of the electric telescopic rod (25) is fixedly connected with the inside of the sealing door plate (24).
7. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 1, characterized in that: The precision needle (9) and the rotary multi-channel micro-injection pump are provided with a connecting hose (26).
8. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 1, characterized in that: One end of the mechanical arm (10) is provided with a base, the base of the mechanical arm (10) is fixedly connected with the side wall of the incubator (1), the precision needle (9) is installed on one end of the mechanical arm (10) close to the circulating conveyor belt (19), and the precision needle (9) is located on the upper part of the circulating conveyor belt (19).
9. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 8, characterized in that: The medicine storage turntable (12) stores medicine bottles (28) of different concentrations of experimental drugs and a water tank (29), the medicine storage turntable (12) drives the medicine bottles (28) and the water tank (29) to rotate, and the position is switched.
10. The temperature-controlled three-dimensional fluorescence-based drug sensitivity detection device according to claim 9, characterized in that: A collecting tank (30) is arranged below the mechanical arm (10), and a waste water tank (31) is arranged at the bottom of the temperature control medicine box (11), and the bottom of the collecting tank (30) is communicated with the inside of the waste water tank (31).