Magnetic immune micro-fluidic chip

Abstract

The utility model discloses a magnetic immune micro-fluidic chip which comprises an upper chip layer, a middle glass substrate and a lower bottom plate, a plurality of detection channels are formed in the chip layer in the width direction, each detection channel comprises a capture area, and a sample inlet channel and a sample outlet channel are formed in the two sides of each capture area respectively; the sample inlet channel is provided with a sample inlet corresponding to the surface of the chip layer, and the sample outlet channel is provided with a sample outlet corresponding to the surface of the chip layer; and magnets are fixed on the bottom plate in positions corresponding to the capturing areas. The bottom plate of the chip is bonded and sealed by the tempered film, so that the chip has the advantages of good sealing performance, good fluorescence permeability, small thickness and the like. The bottom plate is detachably installed, the repeated utilization rate is higher, the chip layer is prone to blockage or cross contamination due to the fact that the caliber of the microfluidic channel is small, the upper side part can be detached, and the bottom plate and the magnet can be repeatedly used.

Description

TECHNICAL FIELD

[0001] The utility model belongs to the technical field of micro -fluidic chip, especially, a kind of magnetic immunomicrofluidic chip. BACKGROUND

[0002] Microfluidic chip is the hotspot field of current micro total analysis system development, microfluidic chip analysis is with chip as operating platform, simultaneously with analytical chemistry as foundation, with micro electro mechanical processing technology as backing, with micro-pipe network as structural feature, with life science as current main application object, it is the focus of current micro total analysis system field development. Its goal is to integrate the function of entire laboratory, including sampling, dilution, reagent addition, reaction, separation, detection etc. on microchip, and can be used repeatedly.

[0003] Microfluidic chip has structural features, internally provided with channel and capture area, and to improve capture effect, magnet is also provided at the bottom of microfluidic chip, corresponding culture solution is improved by the way of adsorbing magnetic beads, and solution capture effect is improved. UTILITY MODEL CONTENT

[0004] The utility model aims at providing a kind of magnetic immunomicrofluidic chip, magnet is also provided at the bottom of microfluidic chip, corresponding culture solution is improved by the way of adsorbing magnetic beads, and solution capture effect is improved.

[0005] To solve the above technical problem, the utility model is realized by the following technical scheme:

[0006] A kind of magnetic immunomicrofluidic chip, including the chip layer of upper layer, glass substrate of middle layer and bottom plate of lower layer;

[0007] The chip layer is provided with a plurality of detection channels along the width direction, and the detection channel includes a capture area, and the capture area is provided with a sample inlet channel and a sample outlet channel on both sides;

[0008] The sample inlet channel has a sample inlet corresponding to the surface of the chip layer, and the sample outlet channel has a sample outlet corresponding to the surface of the chip layer;

[0009] The bottom plate is fixed with a magnet corresponding to the position of the capture area.

[0010] Further, the capture area is semicircular on both sides, and the whole is a waist circular groove, and the arc-shaped ends on both sides are communicated with the sample inlet channel and the sample outlet channel on both sides.

[0011] Further, the chip layer and glass substrate are integrally detachably mounted with the bottom plate, and the bottom plate is provided with a groove body on the coincident side of the glass substrate, for mounting the magnet. The bottom plate side has an inner buckle type sliding groove, and the sliding groove has a slot corresponding to the bottom plate side, for sliding installation of the chip layer and glass substrate along the slot side.

[0012] The chip layer surface has arc-shaped protrusions corresponding to the sample inlet channel, sample outlet channel, and capture area.

[0013] This utility model has the following beneficial effects:

[0014] The chip layer has five parallel detection channels, increasing detection throughput and enabling simultaneous detection of PSG9 levels in five biological samples. The different lengths of the inlet and outlet channels facilitate differentiation between them. The chip's substrate is sealed with tempered glass, offering advantages such as excellent sealing, high fluorescence transmittance, and thinness. The removable substrate allows for greater reusability. Due to the small diameter of the microfluidic channels, the chip layer is prone to clogging or cross-contamination; therefore, the upper part can be removed for reuse of the substrate and magnets. Attached Figure Description

[0015] To more clearly illustrate the technical solutions of the embodiments of this utility model, the accompanying drawings used in the description of the embodiments will be briefly introduced below.

[0016] Figure 1 : Schematic diagram of the overall structure of the chip layer and glass substrate of this utility model.

[0017] Figure 2 : Schematic diagram of the overall disassembly structure of the chip layer and glass substrate of this utility model with the base plate.

[0018] Figure 3 : Schematic diagram of the overall installation structure of the chip layer and glass substrate with the base plate of this utility model.

[0019] The components represented by each number in the attached figure are listed below: chip layer 1, glass substrate 2, base plate 3, capture area 11, sample inlet channel 12, sample outlet channel 13, sample inlet 14, sample outlet 15, magnet 4, groove 31, slide 32, arc-shaped protrusion 16. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments.

[0021] like Figures 1-3 As shown: A magnetic immunomicrofluidic chip includes an upper chip layer 1, a middle glass substrate 2, and a lower substrate 3;

[0022] The chip layer 1 has multiple detection channels along its width direction. Each detection channel includes a capture area 11, and a sample inlet channel 12 and a sample outlet channel 13 are respectively provided on both sides of the capture area 11.

[0023] The sample inlet 14 is arranged on the surface of the chip layer 1 corresponding to the sample inlet channel 12, and the sample outlet 15 is arranged on the surface of the chip layer 1 corresponding to the sample outlet channel 13.

[0024] The preparation method comprises the following steps: etching a capture area, a sample inlet channel groove and a sample outlet channel groove on the surface of the chip layer, and then fixing the etched surface and the glass substrate, and opening the end of the sample inlet channel and the sample outlet channel as the sample inlet and the sample outlet.

[0025] The chip layer is made of PVC, and PVC has the advantages of good light transmission, non-toxicity, durability, low cost, easy availability and bonding with various materials, and has become one of the main materials for manufacturing microfluidic chips.

[0026] The chip layer has five parallel detection channels, and the five channels are designed to improve the detection flux and simultaneously detect the PSG9 content in five biological samples. The chip layer is divided into three parts, including five sample inlets A on the left side of the chip, which are used as the inlets of magnetic beads, flushing reagents and samples. Five sample outlets on the right side of the chip are used as the outlets of un-fixed magnetic beads, flushing waste liquid and sample waste liquid. The diameter of the inlets and outlets is 0.5 mm, the distance from the inlets and outlets to the edge of the chip is 4.5 mm, and the distance between the upper and lower inlets and outlets is 5 mm.

[0027] The sample inlet channel has a width of 0.2 mm, a length of 10 mm and a height of 20 μm, and the sample outlet channel has a width of 0.2 mm, a length of 15 mm and a height of 20 μm. The different lengths of the sample inlet channel and the sample outlet channel are beneficial to distinguish the sample inlet channel and the sample outlet channel. The capture area is located in the center of the chip and is composed of two semicircles with a radius of 1.4 mm and a rectangle with a length of 3.2 mm and a width of 3 mm. The distance between the upper and lower capture areas is 2 mm.

[0028] The bottom plate of the chip is bonded and sealed by a tempered film, and has the advantages of good sealing, good fluorescence permeability and thin thickness.

[0029] The bottom plate 3 is fixed with a magnet 4 corresponding to the position of the capture area 11.

[0030] As shown in Figure 1 The capture area 11 has a semicircular arc shape on both sides, and the whole is a waist circular groove. The arc-shaped ends on both sides are respectively connected to the sample inlet channel 12 and the sample outlet channel 13 on both sides.

[0031] As shown in Figure 2As shown: the chip layer 1 and the glass substrate 2 are integrated and detachably mounted to the base plate 3. A groove 31 is formed on the overlapping side of the base plate 3 and the glass substrate 2 for mounting the magnet 4. The upper groove on the base plate corresponds to the capture area; the groove is 6mm long and 3mm wide. The detachable base plate allows for higher reusability. Due to the small diameter of the microfluidic channels, the chip layer is prone to clogging or cross-contamination. The upper part can be removed, allowing for reuse of the base plate and magnets. The base plate 3 has an inwardly recessed sliding groove 32 on its side, corresponding to a slot on the side of the base plate 3, for the chip layer 1 and the glass substrate 2 to slide along the slot side.

[0032] The surface of chip layer 1 has arc-shaped protrusions 16 corresponding to the sample inlet channel 12, sample outlet channel 13, and capture area 11. This facilitates magnification of the internal channels and capture area for easy observation.

[0033] By integrating a microinjection pump, syringe, injection needle, magnetic immunomicrofluidic chip, dispensing needle, fluorescence microscope, and high-speed imaging CCD into one unit, and using immunofluorescence signal analysis software, qualitative and quantitative detection of PSG9 can be achieved.

[0034] After the magnetic immunomicrofluidic chip was integrated, 1% BSA solution was injected into the chip using a syringe pump to seal the chip channels. 100 μl of resuspended magnetic beads labeled with different antibodies was injected into each channel. Finally, the channels were washed with PBS to remove any unfixed immunomagnetic beads. After washing, the chip was placed under an inverted fluorescence microscope to confirm the fixation of the immunomagnetic beads.

[0035] In this step, the concentration of immunomagnetic beads, the injection flow rate, and the washing time need to be continuously optimized through experiments to ensure that the magnetic beads are well fixed while avoiding waste.

[0036] These embodiments are selected and specifically described in this specification in order to better explain the principles and practical applications of this utility model, so that those skilled in the art can better understand and utilize this utility model.

Claims

1. A magnetic immunomicrofluidic chip, characterized in that: It includes an upper chip layer (1), a middle glass substrate (2), and a lower base plate (3); The chip layer (1) is provided with multiple detection channels along the width direction. Each detection channel includes a capture area (11). A sample inlet channel (12) and a sample outlet channel (13) are respectively provided on both sides of the capture area (11). The sample inlet channel (12) has a sample inlet (14) on the surface of the chip layer (1), and the sample outlet channel (13) has a sample outlet (15) on the surface of the chip layer (1); Magnets (4) are fixed at the positions of the base plate (3) corresponding to the capture area (11).

2. The magnetic immunomicrofluidic chip according to claim 1, characterized in that: The capture area (11) has semi-circular arcs on both sides and is an oval groove as a whole. The arc-shaped ends on both sides are connected to the sample inlet channel (12) and the sample outlet channel (13) on both sides respectively.

3. The magnetic immunomicrofluidic chip according to claim 1, characterized in that: The chip layer (1) and the glass substrate (2) are integrated and can be detachably installed with the base plate (3). A groove (31) is provided on the overlapping side of the base plate (3) and the glass substrate (2) for installing magnets (4).

4. The magnetic immunomicrofluidic chip according to claim 3, characterized in that: The base plate (3) has an inwardly recessed groove (32) on its side, and the groove (32) has a slot on the side of the base plate (3) for the chip layer (1) and the glass substrate (2) to be slidably installed along the slot side.

5. The magnetic immunomicrofluidic chip according to claim 1, characterized in that: The chip layer (1) has arc-shaped protrusions (16) on its surface corresponding to the sample inlet channel (12), sample outlet channel (13) and capture area (11).

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