Microorganism sample inoculation device

Abstract

The utility model provides a microbial sample inoculation device which solves the problems that an existing microbial sample inoculation device is complex in structure and low in operation efficiency. A base is included; a culture dish placing rack, a workbench and a rack are sequentially arranged on the base, a push-out mechanism is arranged below the culture dish placing rack, a linear displacement mechanism is arranged below the workbench, and the push-out mechanism is connected with the moving end of the linear displacement mechanism; a sucker is arranged at the top end of the push-out mechanism and is matched with a bottom plate of the culture dish placing rack to adsorb the culture dish; a sliding groove is formed in the surface of the workbench. The push-out mechanism is in sliding fit with the sliding groove. A cover opener is arranged above the sliding groove, and a clamping mechanism matched with the push-out mechanism to clamp part of the culture dishes is arranged above the cover opener; a streak inoculation mechanism for streak inoculation of the adsorbed culture dishes is connected to the rack in a sliding manner; a sterilization instrument and a test tube placing rack are respectively arranged at two ends of the workbench. The device is widely applied to the technical field of microbial instruments.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of microbiological instruments, and more particularly to a microbiological sample inoculation device. BACKGROUND

[0002] In the field of microbiological experiments, microbiological sample streak inoculation is a key means to obtain pure culture, and the precision and standardization of the operation are directly related to the accuracy of the experimental results. However, the existing microbiological streak inoculation device has many problems in aspects of culture dish taking, process coherence and the like. The traditional device mostly relies on manual or mechanical grabbing to complete culture dish carrying and streak inoculation. Manual operation is not only labor-intensive and low in efficiency, but also easy to cause sample pollution or dish damage, affecting the accuracy of experimental results. Simple mechanical grabbing has problems of being difficult to accurately separate multiple stacked culture dishes, and often causing problems of multiple dishes being grabbed at the same time or deviation in dish taking position, which cannot meet the experimental requirements of automation and high precision.

[0003] In addition, part of the existing device has a scattered layout of each functional module, and the opening of the culture dish, inoculation, sterilization and the like are independent of each other, and need to be transferred by means of a complex mechanical arm or a multi-axis motion mechanism, which is high in equipment cost and control difficulty, and complicated in operation process, and is not high in experimental operation efficiency. CONTENT OF THE INVENTION

[0004] In order to solve the above-mentioned existing problems, the technical scheme adopted by the present application is to provide a microbiological sample inoculation device, which solves the problems of complex structure and low operation efficiency of the existing microbiological sample inoculation device. The device comprises a base, a culture dish placing rack, a workbench and a rack are sequentially arranged on the base, a push-out mechanism is arranged below the culture dish placing rack, a linear displacement mechanism is arranged below the workbench, and the moving end of the linear displacement mechanism is connected with the push-out mechanism; a suction cup is arranged at the top end of the push-out mechanism, and the suction cup is matched with the bottom plate of the culture dish placing rack to adsorb the culture dish; a sliding groove is arranged on the surface of the workbench, and the push-out mechanism is slidingly matched with the sliding groove; an opener is arranged above the sliding groove, and a clamping mechanism for clamping part of the culture dish is arranged above the opener and matched with the push-out mechanism; a streak inoculation mechanism for streak inoculation of the adsorbed culture dish is slidingly connected to the rack; and a sterilization instrument and a test tube placing rack are respectively arranged at both ends of the workbench.

[0005] Preferably, the streak inoculation mechanism performs the streak inoculation operation on the adsorbed culture dish at the end of the sliding groove, and the sterilization instrument, the streak inoculated culture dish and the test tube placing rack are sequentially and side by side arranged along the length direction of the rack.

[0006] Preferably, the push-out mechanism comprises a lifting assembly connected with the moving end of the linear displacement mechanism, an output end of the lifting assembly is connected with a rotating assembly, an output end of the rotating assembly is connected with the suction cup, and the suction cup drives the adsorbed culture dish to slide along the sliding groove.

[0007] Preferably, the rack is provided with a first driving assembly, the line inoculation mechanism comprises a sliding block in sliding connection with the rack, and the output end of the first driving assembly is connected with the sliding block; a support platform is arranged above the sliding block, and the line inoculation assembly is in sliding connection with the support platform.

[0008] Preferably, the support platform is provided with a second driving assembly, the line inoculation assembly comprises a support frame connected with the output end of the second driving assembly and a line lifting motor vertically installed on the support frame, and the output shaft of the line lifting motor is connected with an inoculation ring.

[0009] Preferably, the clamping mechanism comprises a clamp in sliding connection with the top end of the cover opener and a clamp driving assembly for driving the clamp to slide, and the front end of the clamp is provided with a clamping jaw.

[0010] Preferably, the linear displacement mechanism comprises a sliding rail arranged on the base and a displacement block in sliding connection with the sliding rail, and the displacement block is connected with the lifting assembly; the base is further provided with a third driving assembly, and the output end of the third driving assembly is connected with the displacement block.

[0011] Preferably, the base is provided with a placing rack fixing support, the placing rack below the culture dish placing rack is provided with a placing rack rotating driving assembly, and the placing rack fixing support is connected with the output end of the placing rack rotating driving assembly.

[0012] The beneficial effects of the utility model are as follows: the mechanisms are cooperatively operated according to preset programs, the culture dish can be automatically pushed out, the cover can be automatically removed, line inoculation and sterilization operation can be automatically realized, the device has high overall automation degree, manual intervention is not needed, the processing amount per unit time is improved, and the working efficiency is high. The line inoculation mechanism slides linearly along the rack, and sequentially completes test tube sampling, culture dish inoculation, sterilization and other operations, and seamless connection of the processes is realized; compared with the complex path of the traditional equipment, the operation stroke is shortened, repeated movement and time waste caused by scattered layout are avoided, the continuity and overall working efficiency of experimental operation are improved. The whole device has compact structure and reasonable layout, and the occupied space of the device is effectively reduced. BRIEF DESCRIPTION OF DRAWINGS

[0013] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the embodiments or prior art description will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without creative labor.

[0014] Fig. 1 It is a structural schematic view of the utility model;

[0015] Fig. 2 It is a structural schematic view of the utility model from another angle;

[0016] Fig. 3 is a top view schematic diagram of the utility model;

[0017] Explanation of symbols in the drawing:

[0018] 1. base; 2. culture dish placing rack; 3. workbench; 4. rack; 5. push-out mechanism; 6. linear displacement mechanism; 7. clamping mechanism; 8. streaking inoculation mechanism; 9. cap opener; 10. sterilization instrument; 11. test tube placing rack; 12. first driving assembly; 201. placing rack fixing support; 202. placing rack rotary driving assembly; 301. sliding groove; 701. clamp; 702. clamp driving assembly; 801. sliding block; 802. support platform; 803. streaking inoculation assembly; 804. inoculation loop. DETAILED DESCRIPTION

[0019] In order to make the technical problems, technical solutions and beneficial effects to be solved in the present application more clear and explicit, the present application will be further described in detail below in combination with the drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application and not to limit the present application.

[0020] It should be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer" and the like indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only used to facilitate the description of the present application and simplify the description, and therefore cannot be understood as indicating or implying that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore cannot be understood as limiting the present application.

[0021] It should be noted that the terms "second", "push-out" are only used for description purposes, and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the technical features referred to. Therefore, the features limited by "second", "push-out" can explicitly or implicitly include one or more of the features. In the description of the present application, the meaning of "multiple" is two or more, unless otherwise specifically limited.

[0022] A microbial sample inoculation device provided by the embodiment of the present application will be described.

[0023] Please refer to Figs. 1 to 3The utility model provides a structure schematic drawing under different angles for the utility model, the microbial sample inoculation device, including base 1, base 1 is equipped with petri dish rack 2, workstation 3 and rack 4 in proper order, and a plurality of petri dishes to be scribed inoculation are placed in the stacking of petri dish rack 2. The push-out mechanism 5 is equipped under petri dish rack 2, and the linear displacement mechanism 6 is equipped under workstation 3, and the moving end of push-out mechanism 5 is connected with the linear displacement mechanism 6. The top of push-out mechanism 5 is equipped with sucking disc, and sucking disc and the bottom plate of petri dish rack 2 cooperate adsorption petri dish, specifically, the bottom plate is equipped with opening, and push-out mechanism 5 is pushed upwards through the opening and moves petri dish. The surface of workstation 3 is equipped with sliding slot 301, and push-out mechanism 5 and sliding slot 301 slide fit. The unscrambler 9 is equipped above sliding slot 301, and the clamping mechanism 7 that is held part petri dish with push-out mechanism 5 is equipped above unscrambler 9, specifically, push-out mechanism 5 pushes upwards petri dish, when the height of the penultimate petri dish from top to bottom is aligned with clamping mechanism 7, clamping mechanism 7 carries out clamping and fixing to the penultimate petri dish. The scribe inoculation mechanism 8 that scribes the petri dish that is adsorbed is slidably connected on the rack 4 of clamping mechanism 7. Sterilization instrument 10 and test tube rack 11 are respectively equipped at the both ends of workstation 3, and a plurality of test tubes filled with samples are placed on test tube rack 11.

[0024] Specifically, scribe inoculation mechanism 8 carries out scribe inoculation operation to the petri dish that is adsorbed at the end of sliding slot 301, and sterilization instrument 10, the petri dish that is scribed inoculation and test tube rack 11 are sequentially and side by side along the length direction of rack 4. The orderly arrangement of each component along the length direction of rack 4, scribe inoculation mechanism 8 can sequentially complete sampling, scribe inoculation, sterilization along the rack 4, avoids the repeated movement and time waste due to the layout dispersion, improves the coherence and overall work efficiency of experimental operation, and effectively reduces the space occupied by the device.

[0025] Specifically, in use, the push-out mechanism 5 is first started, and its output end pushes the culture dishes on the culture dish rack 2 upward to move up, and when the height of the second last culture dish is aligned with the clamping mechanism 7, the output end of the push-out mechanism 5 stops pushing; the clamping mechanism 7 moves forward to clamp the second last culture dish, and the second last and the culture dishes above it are fixed. The output end of the push-out mechanism 5 returns to the home position, and the lowermost culture dish is adsorbed by the suction cup and falls to the bottom plate of the culture dish rack 2. Subsequently, the linear displacement mechanism 6 is started, and its moving end moves the push-out mechanism 5 and the adsorbed culture dish, the top end of the push-out mechanism 5 and the suction cup cooperate with the sliding groove 301 of the workbench 3, and the adsorbed culture dish is moved on the surface of the workbench 3 through the sliding groove 301. During the movement of the culture dish, the culture dish passes through the lid opener 9 and completes the lid opening operation in the lid opener 9. The culture dish after the lid opening operation moves to the culture dish streaking inoculation position at the end of the sliding groove 301 under the cooperation of the linear displacement mechanism 6 and the push-out mechanism 5. Subsequently, the streaking inoculation mechanism 8 is started, slides along the rack 4 to the test tube rack 11, takes samples from the test tube, and then continues to slide along the rack 4 to the culture dish streaking inoculation position to perform the culture dish streaking inoculation operation. After the inoculation is completed, the streaking inoculation mechanism 8 continues to slide along the rack 4 to the upper side of the sterilization instrument 10 to perform the sterilization operation, and completes the push-out, lid opening and streaking operation of a single culture dish. In actual use, the push-out mechanism 5 cooperates with the clamping mechanism 7 to accurately control the push-out height of the culture dish, so that the second last culture dish is aligned with and fixed by the clamping mechanism 7, and only the lowermost culture dish is adsorbed each time, so that the culture dishes are stably and accurately separated, and the simultaneous movement of multiple culture dishes is avoided. It is suitable for continuous use of multi-layer culture dishes, and improves the reliability and consistency of the culture dish operation. Through the cooperation of the push-out mechanism 5, the linear displacement mechanism 6, the lid opener 9, the clamping mechanism 7 and the streaking inoculation mechanism 8, the automatic push-out, lid opening and streaking operation of the culture dish can be realized, the whole process automation is realized, and the work efficiency is improved.

[0026] Specifically, in this embodiment, the inner wall of the lid opener 9 is provided with an inclined and gradually rising lid opening groove. Under the cooperation of the linear displacement mechanism 6 and the lid opener 9, the culture dish slides along the workbench 3 and enters the lid opener 9, and the lid opening groove completes the lid opening operation of the culture dish.

[0027] Further, the pushing mechanism 5 comprises a lifting assembly connected with the moving end of the linear displacement mechanism 6, the output end of the lifting assembly is connected with a rotating assembly, the output end of the rotating assembly is connected with the suction cup, and the suction cup drives the adsorbed culture dish to slide along the sliding groove 301. In the embodiment, the lifting assembly is a lead screw motor, and the rotating assembly is a stepping motor, the output shaft of the lead screw motor is connected with the bottom surface of the stepping motor, and the output shaft of the stepping motor is connected with the suction cup. The specific working principle of the pushing mechanism 5 is that the lead screw motor is started, the output shaft of the lead screw motor moves in the vertical direction, and drives the stepping motor to rise together; the stepping motor rises, the suction cup contacts and adsorbs the culture dish on the lowermost culture dish placing rack 2, and the suction cup can drive the culture dish to rise and fall in the vertical direction, move in the horizontal direction and rotate under the cooperation of the pushing mechanism 5 and the linear displacement mechanism 6. In other embodiments, the lifting assembly can also be structures including but not limited to air cylinders, hydraulic cylinders and the like, and the rotating assembly can be structures including but not limited to rotating air cylinders, servo motors and the like.

[0028] Specifically, the rack 4 is provided with a first driving assembly 12, the line inoculation mechanism 8 comprises a sliding block 801 slidably connected with the rack 4, and the output end of the first driving assembly 12 is connected with the sliding block 801; a support platform 802 is arranged above the sliding block 801, and a line inoculation assembly 803 is slidably connected with the support platform 802. In the embodiment, the first driving assembly 12 is a synchronous belt transmission mechanism. The first driving assembly 12 comprises first driving wheels and first driven wheels arranged at both ends of the rack 4, the first driving wheels and the first driven wheels are drivingly connected through a first synchronous belt, the first driving wheels are connected with a first driving motor, and the first synchronous belt is connected with the sliding block 801. The specific working principle of the first driving assembly 12 is that the first driving motor is started to drive the first driving wheels, the first synchronous belt and the first driven wheels to rotate, so as to drive the sliding block 801 connected with the first synchronous belt to slide, and the sliding block 801 drives the line inoculation assembly 803 to move along the length direction (horizontal X-axis) of the rack 4 through the support platform 802.

[0029] Specifically, the second driving assembly is arranged on the support platform 802, the scribe inoculation assembly 803 comprises a support frame connected with an output end of the second driving assembly and a scribe lifting motor vertically arranged on the support frame, and an inoculation ring 804 is connected with an output shaft of the scribe lifting motor. In the embodiment, the driving direction of the second driving assembly is perpendicular to the driving direction of the first driving assembly 12, and the second driving assembly is similar to the first driving assembly 12 in working principle and structure, and will not be described here. Under the action of the second driving assembly, the scribe inoculation assembly 803 moves along the width direction (horizontal Y axis) of the rack 4. The specific working principle of the scribe inoculation assembly 803 is as follows: when sampling is needed, the output shaft of the scribe lifting motor moves downward (vertical Z axis), so that the inoculation ring 804 enters the inside of the test tube to sample, and then the output shaft of the scribe lifting motor moves upward (vertical Z axis), so that sampling is completed; when scribe inoculation operation is needed, the output shaft of the scribe lifting motor moves downward, so that the inoculation ring 804 scribes on the surface of the culture dish, and then the output shaft of the scribe lifting motor moves upward, so that scribe inoculation is completed. Through the first driving assembly 12 and the second driving assembly, the scribe inoculation assembly 803 realizes position adjustment in the horizontal direction of X axis and Y axis; through the scribe lifting motor, the inoculation ring 804 realizes position adjustment in the vertical direction of Z axis.

[0030] Specifically, the clamping mechanism 7 comprises a clamping device 701 slidably connected with the top end of the cover opener 9 and a clamping device driving assembly 702 driving the clamping device 701 to slide, and the clamping device 701 is provided with a clamping jaw at the front end. In the embodiment, the clamping device driving assembly 702 comprises a clamping device driving fixed support arranged on the base 1 and a clamping device driving motor vertically arranged on the clamping device driving fixed support, and the output shaft of the clamping device driving motor moves up and down in the vertical direction; the top end of the output shaft of the clamping device driving motor is connected with a lifting rod, and the lifting rod moves up and down in the vertical direction under the action of the output shaft of the clamping device driving motor; the top end of the lifting rod and the clamping device 701 are connected through a conversion block, and the conversion block is rotatably connected with the top end of the lifting rod and the clamping device 701 at both ends, so as to convert the movement of the lifting rod in the vertical direction into the movement of the clamping device 701 in the horizontal direction. In other embodiments, the clamping device driving assembly 702 can be, but is not limited to, a linear motor, an air cylinder, a hydraulic cylinder, a screw nut mechanism and a synchronous belt transmission mechanism.

[0031] Specifically, the linear displacement mechanism 6 comprises a sliding rail arranged on the base 1 and a displacement block slidably connected with the sliding rail, and the displacement block is connected with the lifting assembly; the base 1 is further provided with a third driving assembly, and an output end of the third driving assembly is connected with the displacement block. The third driving assembly is similar to the first driving assembly 12 in working principle and structure, and will not be described here.

[0032] Specifically, the base 1 is provided with a placing rack fixing support 201, the placing rack 2 is provided below with a placing rack rotating driving assembly 202, and the placing rack fixing support 201 is connected with the placing rack rotating driving assembly 202. In the embodiment, the placing rack rotating driving assembly 202 is a synchronous belt transmission mechanism, and the main shaft of a driven wheel in the synchronous belt transmission mechanism is connected with the center of the bottom plate of the placing rack fixing support 201. In addition, the placing rack rotating driving assembly 202 can also be a driving structure including but not limited to a rotary motor.

[0033] The working process of the utility model: when using, the push-out mechanism 5 is started first, the output end thereof pushes the petri dish on the petri dish placing rack 2 upwards, when the height of the second last petri dish is aligned with the clamping mechanism 7, the output end of the push-out mechanism 5 stops pushing; the clamping mechanism 7 moves forward and clamps the second last petri dish. Subsequently, the output end of the push-out mechanism 5 is homed, the lowermost petri dish is adsorbed by the suction cup and falls to the bottom plate of the petri dish placing rack 2 together. The linear displacement mechanism 6 is started, the moving end thereof drives the push-out mechanism 5 and the adsorbed petri dish to move, and the push-out mechanism 5 drives the adsorbed petri dish to move on the surface of the workbench 3 through the sliding groove 301. In the moving process of the petri dish, the petri dish passes through the cover opener 9 and completes the cover removal operation in the cover opener 9, and the petri dish after cover removal moves to the petri dish streaking inoculation position at the end of the sliding groove 301 under the cooperation of the linear displacement mechanism 6 and the push-out mechanism 5. Subsequently, the streaking inoculation mechanism 8 is started, slides along the rack 4 to the test tube placing rack 11, takes samples from the test tube, and then continues to slide along the rack 4 to the petri dish streaking inoculation position to perform the streaking inoculation operation on the petri dish. After inoculation, the streaking inoculation mechanism 8 continues to slide along the rack 4 to above the sterilization instrument 10 to perform the sterilization operation, and completes the push-out, cover removal and streaking operation on a single petri dish.

[0034] In the utility model, the mechanisms are cooperated according to the preset program, can automatically realize the push-out, cover removal, streaking inoculation and sterilization operation of the petri dish, the device is high in overall automation degree, does not need manual intervention, improves the processing capacity in unit time, and is high in working efficiency. The streaking inoculation mechanism 8 linearly slides along the rack 4, sequentially completes the test tube sampling, petri dish inoculation and sterilization operations, realizes the seamless connection of the process; compared with the complex path of the traditional equipment, the operation stroke is shortened, the repeated movement and time waste caused by the scattered layout are avoided, and the continuity and overall working efficiency of the experimental operation are improved. The whole device is compact in structure and reasonable in layout, effectively reduces the space occupied by the device.

[0035] The above-described embodiments are only used to illustrate the technical solutions of the present application, but not limit them; although the present application is described in detail with reference to the foregoing embodiments, those skilled in the art should understand that: it can still modify the technical solutions recorded in the foregoing embodiments, or make equivalent replacement to part of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present application, and should be included in the protection scope of the present application.

Claims

1. A microbial sample inoculation device, comprising a base, characterized in that: The base is provided with a petri dish rack, a workbench, and a frame in sequence. A push-out mechanism is located below the petri dish rack, and a linear displacement mechanism is located below the workbench. The push-out mechanism is connected to the moving end of the linear displacement mechanism. A suction cup is located at the top of the push-out mechanism, which cooperates with the bottom plate of the petri dish rack to adsorb petri dishes. A sliding groove is provided on the surface of the workbench, and the push-out mechanism slides within the groove. A cap opener is located above the groove, and a clamping mechanism, which cooperates with the push-out mechanism, is located above the cap opener to hold a portion of the petri dishes. A streaking mechanism for streaking and inoculating the adsorbed petri dishes is slidably connected to the frame. A sterilizer and a test tube rack are located at both ends of the workbench.

2. The microbial sample inoculation device as described in claim 1, characterized in that: The streaking inoculation mechanism performs streaking inoculation on the adsorbed petri dish at the end of the chute, and the sterilizer, the streaked petri dish, and the test tube rack are arranged side by side along the length of the frame.

3. The microbial sample inoculation device as described in claim 1, characterized in that: The ejection mechanism includes a lifting component connected to the moving end of the linear displacement mechanism, a rotating component connected to the output end of the lifting component, and a suction cup connected to the output end of the rotating component. The suction cup drives the adsorbed culture dish to slide along the groove.

4. The microbial sample inoculation device as described in claim 1, characterized in that: The frame is provided with a first drive assembly, and the marking and inoculation mechanism includes a sliding block that is slidably connected to the frame. The output end of the first drive assembly is connected to the sliding block. A support platform is provided above the sliding block, and the marking and inoculation assembly is slidably connected to the support platform.

5. The microbial sample inoculation device as described in claim 4, characterized in that: The support platform is provided with a second drive component. The marking and inoculation component includes a support frame connected to the output end of the second drive component and a marking and lifting motor vertically mounted on the support frame. The output shaft of the marking and lifting motor is connected to an inoculation ring.

6. The microbial sample inoculation device as described in claim 1, characterized in that: The clamping mechanism includes a clamp that is slidably connected to the top of the lid opener and a clamping drive assembly that drives the clamp to slide. The front end of the clamp is provided with a gripper.

7. The microbial sample inoculation device as described in claim 3, characterized in that: The linear displacement mechanism includes a slide rail disposed on the base and a displacement block slidably connected to the slide rail, the displacement block being connected to the lifting assembly; the base is also provided with a third drive assembly, the output end of the third drive assembly being connected to the displacement block.

8. The microbial sample inoculation device as described in claim 1, characterized in that: The base is provided with a placement rack fixing bracket, and a placement rack rotation drive assembly is provided below the petri dish placement rack. The placement rack fixing bracket is connected to the output end of the placement rack rotation drive assembly.

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