Portable anaerobic sampling and carrying device

Abstract

The utility model discloses a portable anaerobic sampling and carrying device. The portable anaerobic sampling and carrying device comprises a main body container with a double-layer structure, a gas replacement module integrated with a deoxidation function and a monitoring module with a visual oxygen concentration feedback function, a pressure-resistant glass bottle is adopted as the main body container, the inner layer of the pressure-resistant glass bottle is coated with a nano titanium dioxide photocatalytic deoxidation film, and a bottle opening of the pressure-resistant glass bottle is sealed through a butyl rubber sealing plug; a diversion channel is arranged in the butyl rubber sealing plug, one end of the diversion channel is inserted into the bottle, the other end extends out of the bottle, and the tail end is connected with a gas replacement module; the gas replacement module adopts a three-way valve to integrate a one-way exhaust port and a sampling needle interface; and the monitoring module comprises a transparent observation window embedded with an oxygen-sensitive color-changing film. By adopting a triple mechanism of gas replacement, chemical deoxidation and photocatalysis, the limitation that the traditional device depends on an external gas source is solved, the whole-process anaerobic control of the sampling process is realized, and the device is suitable for field and laboratory scenes.

Description

Technical Field

[0001] This utility model relates to the field of microbial and environmental detection technology, and in particular to a portable anaerobic sampling and carrying device. Background Technology

[0002] The collection of anaerobic samples (such as soil / sediment, water, tissue blocks) requires strict exclusion of oxygen interference in order to maintain the activity and original state of anaerobic microorganisms in the samples.

[0003] Current methods for collecting anaerobic samples often employ bulky metal containers or complex vacuum systems, which have the following drawbacks:

[0004] 1. Traditional equipment relies on a continuous supply of inert gas, making field operation difficult;

[0005] 2. The complex sealing structure results in a short sampling time window (<15 seconds);

[0006] 3. Unable to monitor changes in oxygen concentration in real time.

[0007] Therefore, there is a need for a portable anaerobic sampling and carrying device that is simple in structure, low in cost, and can maintain a stable anaerobic environment. Utility Model Content

[0008] This invention addresses the aforementioned technical problems by providing a portable anaerobic sampling and carrying device suitable for field and laboratory settings.

[0009] To achieve the above objectives, this utility model provides the following technical solution:

[0010] A portable anaerobic sampling and carrying device includes a main container with a double-layer structure, a gas replacement module with integrated deoxygenation function, and a monitoring module with visualized oxygen concentration feedback. The main container is a pressure-resistant glass bottle, the inner layer of which is coated with a nano-titanium dioxide photocatalytic deoxygenation membrane. The bottle opening is sealed with a butyl rubber stopper. The butyl rubber stopper has a built-in flow channel, one end of which is inserted into the bottle, and the other end extends to the outside of the bottle, with the end connected to the gas replacement module. The gas replacement module uses a three-way valve to integrate a one-way exhaust port and a sampling needle interface. The monitoring module includes a transparent observation window with an embedded oxygen-sensitive color-changing film.

[0011] Furthermore, the pressure-resistant glass bottle is made of high borosilicate glass with a thickness of 3-5mm and can withstand pressure of 0.1-0.5MPa.

[0012] Furthermore, the thickness of the nano-titanium dioxide photocatalytic deoxygenation film is 5-10 μm.

[0013] Furthermore, the mouth of the pressure-resistant glass bottle is made of an inverted conical ground joint.

[0014] Furthermore, the butyl rubber sealing plug has a thickness of 8-10 mm, and a silicone sealing ring is connected to its bottom.

[0015] Furthermore, the end of the three-way valve connected to the flow channel is provided with a spiral gas pre-storage chamber, which contains a solid deoxidizer.

[0016] Furthermore, the pressure-resistant glass bottle has a transparent observation window on its body, and the oxygen-sensitive color-changing film is located inside the transparent observation window and embedded in the groove of the nano-titanium dioxide photocatalytic deoxygenation film.

[0017] Furthermore, the color development threshold of the oxygen-sensitive color-changing film is set to an oxygen concentration of 0.5%, and the color change range gradually changes from colorless to dark blue.

[0018] Furthermore, the butyl rubber sealing plug is equipped with a miniature pressure balancing valve.

[0019] Furthermore, the butyl rubber sealing plug is fitted with a temperature and humidity sensor, and the probe of the temperature and humidity sensor 304 extends into the bottle.

[0020] Compared with the prior art, the beneficial effects of this utility model are as follows:

[0021] The portable anaerobic sampling and carrying device provided by this utility model has a simple structure and low cost. By integrating a gas replacement module and a visual monitoring module on the main container with a double-layer structure, it realizes full anaerobic control of the sampling process. By adopting a triple mechanism of gas replacement + chemical deoxygenation + photocatalysis, it solves the limitation of traditional devices that rely on external gas sources and can maintain a stable anaerobic environment, making it suitable for field and laboratory scenarios. Attached Figure Description

[0022] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0023] Figure 1 A front view of the portable anaerobic sampling and carrying device provided in an embodiment of this utility model.

[0024] Figure 2 A cross-sectional view of the portable anaerobic sampling and carrying device provided in an embodiment of this utility model.

[0025] Explanation of reference numerals in the attached figures:

[0026] 101. Pressure-resistant glass bottle; 102. Nano titanium dioxide photocatalytic deoxidation membrane; 103. Butyl rubber sealing stopper; 104. Flow channel; 105. Silicone sealing ring.

[0027] 201. Three-way valve; 202. One-way exhaust port; 203. Sampling needle interface;

[0028] 301. Transparent observation window; 302. Oxygen-sensitive color-changing film; 303. Miniature pressure balancing valve; 304. Temperature and humidity sensor. Detailed Implementation

[0029] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments.

[0030] like Figure 1-2 As shown, this utility model provides a portable anaerobic sampling and carrying device, including a main container with a double-layer structure, a gas replacement module with integrated deoxygenation function, and a monitoring module with visualized oxygen concentration feedback. Wherein:

[0031] The main container is a pressure-resistant glass bottle 101. Specifically, the pressure-resistant glass bottle 101 is made of high borosilicate glass with a thickness of 3-5mm and can withstand a pressure of 0.1-0.5MPa.

[0032] The inner layer of the pressure-resistant glass bottle 101 is coated with a nano-titanium dioxide photocatalytic deoxygenation film 102. Specifically, the nano-titanium dioxide photocatalytic deoxygenation film 102 has a thickness of 5-10 μm, can be cured by the sol-gel method, and can catalytically decompose oxygen under ultraviolet light (wavelength 254 nm). The nano-titanium dioxide photocatalytic deoxygenation film 102 needs to be regenerated periodically by ultraviolet light irradiation to maintain deoxygenation efficiency.

[0033] The pressure-resistant glass bottle 101 has its mouth sealed with a butyl rubber sealing stopper 103; specifically, the mouth of the pressure-resistant glass bottle 101 adopts an inverted conical ground joint. The butyl rubber sealing stopper 103 has a thickness of 8-10mm, and a silicone sealing ring 105 is connected to its bottom to enhance airtightness. All interfaces can be protected by a conical seal + O-ring double protection to ensure a leakage rate of <1×10⁻⁶. -9 Pa·m 3 / s.

[0034] The butyl rubber sealing plug 103 has a built-in flow channel 104. If a glass conduit with an inner diameter of 2-3 mm is used, one end of the flow channel 104 is inserted into the bottle through the sealing plug, and the other end extends to the outside of the bottle, with the end connected to the gas replacement module.

[0035] The gas replacement module integrates a three-way valve 201 with a one-way exhaust port 202 and a sampling needle interface 203. Specifically, the three-way valve 201 is made of 316 stainless steel, and the end connected to the flow channel 104 has a spiral gas pre-storage chamber containing a solid deoxidizer. The one-way exhaust port 202 is connected to a one-way exhaust valve. The sampling needle interface 203 uses a Luer connector to connect to the sampling needle. Rotating the three-way valve 201 to the pre-deoxygenation position releases nitrogen from the solid deoxidizer 205 to replace the air in the container. Rotating the three-way valve 201 to the sampling needle interface 203 position allows sediment samples to be injected through the sampling needle interface 203. Rotating the three-way valve 201 to the equilibrium position allows exhaust gas to be discharged from the three-way valve through the one-way exhaust port 202. The three-way valve enables rapid switching between "deoxygenation-sampling-equilibrium" modes, suitable for laboratory gas handling scenarios. The one-way exhaust port 202 serves a one-way exhaust function: it only allows gas to be discharged in one direction (such as when the internal pressure is too high), but does not allow outside air to enter in the opposite direction.

[0036] The monitoring module includes a transparent observation window 301 with an embedded oxygen-sensitive color-changing film 302. Specifically, the pressure-resistant glass bottle 101 has a transparent observation window 301 on its body, the size of which can be, for example, 3cm × 2cm, for observing the color change of the internal oxygen-sensitive color-changing film 302. The oxygen-sensitive color-changing film 302 is located inside the transparent observation window 301 and is embedded in the groove of the nano-titanium dioxide photocatalytic deoxygenation film 102. The oxygen-sensitive color-changing film 302 can be a ruthenium complex film based on the fluorescence quenching principle, adhered to the inside of the observation window. Further, the color development threshold of the oxygen-sensitive color-changing film 302 is set to an oxygen concentration of 0.5%, and the color change range gradually changes from colorless to deep blue.

[0037] In a preferred embodiment, the butyl rubber sealing plug 103 is provided with a miniature pressure balancing valve 303. The miniature pressure balancing valve 303 can play a bidirectional role: when the internal pressure of the system is higher or lower than the external air pressure, it automatically opens to balance the airflow (such as drawing in outside air or releasing internal gas) to maintain pressure stability.

[0038] The use of a miniature pressure balancing valve 303 and a one-way exhaust port 202 works in tandem to prevent negative pressure and overpressure, as well as dust and backflow. The pressure balancing valve balances small pressure fluctuations (such as pressure changes caused by temperature variations), while the one-way exhaust port has a higher opening pressure threshold (triggered when exceeding a safe value). Operating logic: Normal state: The pressure balancing valve maintains a slight pressure balance, and the one-way exhaust port is closed. Slight increase in internal pressure: The balancing valve automatically exhausts gas to maintain pressure stability. Negative pressure: Outside air is drawn in through the pressure balancing valve, while the one-way exhaust port prevents dust, liquids, etc., from entering in reverse. Abnormally high internal pressure (exceeding the balancing valve's capacity): The one-way exhaust port opens, rapidly releasing gas to prevent explosions and other hazards, while preventing backflow of outside air.

[0039] To further monitor the temperature and humidity inside the bottle, in a preferred embodiment, the butyl rubber sealing plug 103 is fitted with a temperature and humidity sensor 304. The probe of the temperature and humidity sensor 304 extends into the bottle, and the data can be transmitted to an external display terminal via a wire. For example, the model of the temperature and humidity sensor is SHT30-DIS-B, with an accuracy of ±2%RH and ±0.3℃.

[0040] This invention discloses a portable anaerobic sampling and carrying device. By integrating a gas replacement module and a visual monitoring module into a double-layered main container, it achieves full anaerobic control during the sampling process. The core innovation lies in employing a triple mechanism of gas replacement, chemical deoxygenation, and photocatalysis, overcoming the limitations of traditional devices that rely on external gas sources. Testing shows that it can maintain an anaerobic state for 72 hours at 30℃, with oxygen concentration fluctuations ≤0.2%, making it suitable for environmental monitoring, clinical testing, and other fields.

[0041] The above embodiments are only used to illustrate the technical solutions of this utility model, and are not intended to limit it. Although this utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. However, these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of this utility model.

Claims

1. A portable anaerobic sampling and carrying device, characterized in that, The device includes a main container with a double-layer structure, a gas replacement module with integrated deoxygenation function, and a monitoring module with visualized oxygen concentration feedback. The main container is a pressure-resistant glass bottle (101), the inner layer of which is coated with a nano-titanium dioxide photocatalytic deoxygenation membrane (102). The bottle mouth of the pressure-resistant glass bottle (101) is sealed by a butyl rubber sealing stopper (103). The butyl rubber sealing stopper (103) has a built-in flow channel (104), one end of which is inserted into the bottle and the other end extends to the outside of the bottle, with the end connected to the gas replacement module. The gas replacement module uses a three-way valve (201) to integrate a one-way exhaust port (202) and a sampling needle interface (203). The monitoring module includes a transparent observation window (301) with an embedded oxygen-sensitive color-changing film (302).

2. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The pressure-resistant glass bottle (101) is made of high borosilicate glass with a thickness of 3-5mm and can withstand pressure of 0.1-0.5MPa.

3. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The thickness of the nano-titanium dioxide photocatalytic deoxidation film (102) is 5-10 μm.

4. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The pressure-resistant glass bottle (101) has an inverted conical ground joint at its mouth.

5. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The butyl rubber sealing plug (103) has a thickness of 8-10 mm and a silicone sealing ring (105) is connected to its bottom.

6. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The three-way valve (201) is connected to the flow channel (104) at one end, which is provided with a spiral gas pre-storage chamber, and the spiral gas pre-storage chamber contains a solid deoxidizer.

7. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The pressure-resistant glass bottle (101) has a transparent observation window (301) on its body. The oxygen-sensitive color-changing film (302) is located inside the transparent observation window (301) and is embedded in the groove of the nano-titanium dioxide photocatalytic deoxygenation film (102).

8. The portable anaerobic sampling and carrying device according to claim 7, characterized in that, The color development threshold of the oxygen-sensitive color-changing film (302) is set to an oxygen concentration of 0.5%, and the color change range gradually changes from colorless to dark blue.

9. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The butyl rubber sealing plug (103) is equipped with a miniature pressure balancing valve (303).

10. The portable anaerobic sampling and carrying device according to claim 1, characterized in that, The butyl rubber sealing plug (103) is fitted with a temperature and humidity sensor (304), and the probe of the temperature and humidity sensor (304) extends into the bottle.

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