Device for yeast experiment
By designing a yeast experimental device consisting of a lid and a base, the problem of uneven yeast spot arrangement was solved, achieving uniform distribution of yeast spots, improving experimental efficiency and data reliability, and ensuring the accuracy of scientific research data.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Utility models(China)
- Current Assignee / Owner
- 百奥曼(北京)科技有限公司
- Filing Date
- 2025-03-31
- Publication Date
- 2026-04-17
AI Technical Summary
In yeast experiments, existing techniques make it difficult to neatly arrange yeast spots, leading to duplicate images and inaccurate data processing, which affects the credibility of the paper.
Design a yeast experimental apparatus consisting of a lid and a base. The lid has raised pillars, and the base has corresponding holes. The yeast can be neatly arranged by the fitting of the pillars and holes. The apparatus can be prepared using metal, plastic, or glass materials.
This method achieves uniform and precise distribution of yeast spots, improves experimental efficiency, ensures the authenticity and high quality of data, avoids image processing errors, and enhances the reliability of scientific research.
Smart Images
Figure CN224133018U_ABST
Abstract
Description
Technical Field
[0001] This utility model relates to experimental apparatus for yeast, and more particularly to experimental apparatus for yeast single hybridization, yeast double hybridization or other yeast hybridization, belonging to the field of experimental apparatus for yeast. Background Technology
[0002] Many scientific papers in the field of biology have been found to contain duplicate images. Due to data processing negligence or other reasons, some researchers have produced identical images in one or more papers despite different experimental conditions. This lack of rigor not only affects the authenticity of scientific data but also easily misleads the scientific community and the public's understanding of experimental results. This is particularly true in experiments using yeast, such as yeast two-hybrid experiments, which require displaying a large number of yeast growth dots. These images are extremely similar, with some even indistinguishable to the naked eye. The similar shapes of the yeast dots make it easy to misplace them during the cutting and layout process, leading to duplicate images. Since it is difficult to manually arrange a large number of yeast dots neatly on the culture medium for a unified display, researchers often crop each dot. This operation easily results in image duplication, affecting the credibility of the paper and potentially leading to questioning or even retraction. Therefore, there is an urgent need to develop an experimental device that can neatly arrange all the yeast samples to be tested onto a single large image to solve the problems of misuse of yeast images and the excessive workload of cropping yeast images. Utility Model Content
[0003] The purpose of this invention is to provide an apparatus for yeast experiments, which can neatly arrange all the yeast samples to be tested on a large image.
[0004] To achieve the above objectives, the main technical solutions adopted by this utility model include:
[0005] An apparatus for yeast experiments, comprising a lid and a base that are independent of each other and can be interlocked, wherein the lid is provided with a plurality of raised posts, and the base is provided with holes corresponding to the raised posts on the lid.
[0006] In a preferred embodiment of this utility model, the shape, position, and number of the pillars on the lid match the holes on the base. Therefore, when the lid is placed on the base, the lid and the base are locked together by the matching of the pillars and the holes.
[0007] In a preferred embodiment of this utility model, the diameter of the hole in the pillar on the cover is slightly smaller than the diameter of the hole in the base, so that the pillar can be inserted into the hole; in addition, the length of the pillar can be the same as or greater than the depth of the hole.
[0008] In a preferred embodiment of this utility model, the protruding pillars are arranged neatly on the cover in a horizontal and vertical row, and correspondingly, the holes on the base are also arranged neatly in a horizontal and vertical row.
[0009] In a preferred embodiment of this utility model, the number of columns or holes can be set according to the needs of the yeast two-hybrid experiment. Considering that it is generally customary to dilute four concentration gradients when conducting the yeast two-hybrid experiment, 8*8 yeast spots are designed. Therefore, as a preferred embodiment, the columns or holes are arranged neatly in rows of 8 each.
[0010] In a preferred embodiment of this utility model, a handle is provided on the lid; the handle facilitates placing the lid on the base or removing the lid from the base; the shape of the handle can be designed in any shape according to ergonomics, as long as it is convenient to hold by hand.
[0011] In a preferred embodiment of this utility model, the cover, pillar, handle or base, and holes can be made of metal, plastic, or glass materials; wherein, the metal is preferably stainless steel.
[0012] This invention can be applied to any yeast experiment that requires photocopying, such as yeast one-hybrid, yeast two-hybrid, or yeast three-hybrid experiments.
[0013] For reference, this utility model provides a method for applying the device in a yeast two-hybrid experiment, including:
[0014] Add different yeast solutions to the different holes on the base; attach the cap to the base so that the post on the cap is inserted into the matching hole on the base and dips into the yeast solution in the hole; remove the cap from the base and then transfer the yeast solution dipped by the post on the cap onto the yeast culture medium.
[0015] This novel device can quickly and neatly spot yeast cells during yeast one-hybrid, two-hybrid, or three-hybrid experiments, ensuring uniform and precise distribution of yeast cells and avoiding errors caused by human factors. It allows researchers to perform high-precision spotting operations in a short time, greatly improving experimental efficiency and ensuring that each spot is arranged in an orderly manner, allowing for full-image display, saving data processing time and increasing reliability. At the same time, it also avoids potential problems caused by experimental repetition and improper image processing, allowing researchers to focus more on other aspects of the experiment, thereby ensuring the authenticity and high quality of data in scientific papers. Attached Figure Description
[0016] Figure 1 This is a schematic diagram of the lid in the device of this utility model.
[0017] Figure 2 This is a cross-sectional view of the lid in the device of this utility model.
[0018] Figure 3 This is a schematic diagram of a cover with a handle in the device of this utility model.
[0019] Figure 4 This is a schematic diagram of the base in the device of this utility model.
[0020] Figure 5 This is a cross-sectional view of the base in the device of this utility model.
[0021] Figure 6 This is a schematic diagram of the cover and base of the device being fastened together.
[0022] Figure 7 This is the experimental effect of using the yeast experimental device of this utility model to transfer yeast culture liquid onto yeast culture medium (all yeast samples to be tested are neatly arranged on a large image).
[0023] 1-Lid, 2-Post, 3-Base, 4-Hole, 5-Handle. Detailed Implementation
[0024] The present invention will be further described below with reference to specific embodiments, and the advantages and features of the present invention will become clearer as a result of the description. However, it should be understood that the embodiments described are merely exemplary and do not constitute any limitation on the scope of the present invention. Those skilled in the art should understand that modifications or substitutions to the details and form of the technical solution of the present invention can be made without departing from the spirit and scope of the present invention, but such modifications or substitutions all fall within the protection scope of the present invention.
[0025] according to Figures 1-6 As can be seen, the device for yeast experiments provided by this utility model consists of a lid 1 and a base 3 that are independent of each other and can be fastened together. The lid 1 is provided with a plurality of protruding pillars 2, and the base 3 is provided with holes 4 that correspond to the protruding pillars on the lid 1.
[0026] In a preferred embodiment of this utility model, the shape, position, and number of the pillars 2 on the cover 1 match the holes 4 on the base 3. Therefore, when the cover 1 is placed on the base 3, the cover 1 and the base 3 are locked together by the matching of the pillars 2 and the holes 4.
[0027] In a preferred embodiment of this utility model, the diameter of the hole in the column 2 is slightly smaller than the diameter of the hole in the hole 4, so that the column 2 can be inserted into the hole 4; in addition, the length of the column 2 can be the same as or greater than the depth of the hole 4.
[0028] In a preferred embodiment of this utility model, the protruding pillars 2 on the cover 1 are arranged neatly in a horizontal and vertical row, and correspondingly, the holes 4 on the base 3 are also arranged in a horizontal and vertical row.
[0029] In a preferred embodiment of this utility model, the number of columns 2 or holes 4 can be set according to the needs of the yeast two-hybrid experiment. Considering that it is generally customary to dilute 4 concentration gradients when conducting the yeast two-hybrid experiment, 8*8 yeast spots are designed. Therefore, as a preferred embodiment, the columns 2 or holes 4 are neatly arranged on the lid 1 or base 3 according to the rule of 8 rows per row, for a total of 8 rows.
[0030] according to Figure 3 As can be seen, a handle 5 can be provided on the cover 1, which makes it convenient to put the cover 1 on the base 3 or to remove the cover 1 that is on the base 3.
[0031] The device of this utility model can be made of metal, plastic or glass materials to make the cover 1, column 2, handle 5 or base 3 and hole 4; wherein, the metal is preferably stainless steel.
[0032] As an example, the application method of this invention's device in a yeast two-hybrid experiment includes:
[0033] Add different yeast solutions to the different holes 4 on the base 3 respectively; put the lid 1 on the base 3 so that the column 2 on the lid 1 is inserted into the corresponding hole 4 on the base 3 and dips into the yeast solution in the hole 4; remove the lid 1 from the base 3, and then transfer the yeast solution dipped by the column 2 of the lid 1 onto the yeast culture medium.
[0034] Figure 7 This is an experimental result image of yeast culture being transferred onto a yeast culture medium when using the device of this invention for a yeast two-hybrid experiment. As can be seen from the experimental result image, using the device of this invention for yeast two-hybrid experiments can neatly arrange all the yeast samples to be tested on a large image, eliminating the need to crop numerous yeast hybridization images one by one.
Claims
1. An apparatus for yeast experimentation, characterized by, The device consists of a cover (1) and a base (3) that are independent of each other and can be fastened together; wherein, the cover (1) is provided with a plurality of protruding pillars (2), and the base (3) is provided with corresponding holes (4) that fit with the protruding pillars on the cover (1).
2. The apparatus of claim 1, wherein, The shape, position, and number of the pillars (2) on the cover (1) match the holes (4) in the base (3).
3. The apparatus of claim 1 or 2, wherein, The diameter of the hole in the column (2) is smaller than the diameter of the hole (4).
4. The apparatus of claim 1 or 2, wherein, The length of the column (2) is the same as or greater than the depth of the hole (4).
5. The apparatus of claim 1, wherein, The protruding pillars (2) are arranged neatly on the cover (1) according to the rule of horizontal and vertical rows, and correspondingly, the holes (4) on the base (3) are also arranged neatly according to the corresponding rule of horizontal and vertical rows.
6. The apparatus of claim 1 or 5, wherein, The pillars (2) or holes (4) are arranged in a neat row on the cover (1) or base (3) with eight pillars in each row.
7. The apparatus of claim 1, wherein, A handle (5) is provided on the lid (1).
8. The apparatus of claim 1, wherein, The device is made of metal, plastic or glass materials.
9. The apparatus of claim 8, wherein, The metal in question is stainless steel.