U-shaped tube cell separation device

Abstract

The utility model relates to the technical field of biology, in particular to a U-shaped tube cell separation device which comprises a bottom plate, a top plate is arranged at the upper end of the bottom plate, a negative pressure extraction mechanism is arranged on one side, located on the top plate, of the bottom plate, a lifting mechanism is arranged on the other side of the bottom plate, a containing groove is formed in the top plate, and a U-shaped cell filter tube is arranged in the containing groove. And the bottom of the U-shaped cell filter tube is supported by a lifting mechanism. The U-shaped tube cell separation device has the advantages of being simple in structure, convenient to operate, high in cell separation efficiency, small in cell damage and the like, can be suitable for separation of various types of cells through different microfiltration membranes, and meets the requirements of the fields of biological experiments, cell therapy and the like.

Description

Technical Field

[0001] This utility model relates to the field of biotechnology, specifically to a U-shaped tube cell separation device. Background Technology

[0002] In the field of biomedical research, the efficient and gentle separation and collection of cells is undoubtedly a core component of biological experiments and cell detection processes, and its importance is self-evident. Traditional cell separation methods, despite their long history, are often limited by cumbersome procedures, low separation efficiency, and severe cell damage. Taking centrifuge separation as an example, this technology utilizes the differences in cell density. The powerful centrifugal force generated by the high-speed rotation of the centrifuge causes cells to naturally stratify within the centrifuge tube, thus achieving separation. Cells such as red blood cells and white blood cells, due to their different densities, will naturally distribute themselves into different layers during centrifugation.

[0003] However, this method is not without its flaws. The similarity in cell size and density often becomes a key factor affecting the purity of separation, making precise separation particularly difficult for certain cell types or samples with complex compositions. Moreover, the mechanical stress generated during high-speed centrifugation can cause irreversible damage to cells, thereby weakening their activity and function, which is undoubtedly a major obstacle to subsequent experimental research.

[0004] Therefore, there is a need for a new type of cell separation device that can simplify the operation process, improve separation efficiency, and reduce cell damage, so as to better serve biomedical research. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a U-shaped tube cell separation device, including a base plate, a top plate at the upper end of the base plate, a negative pressure extraction mechanism on one side of the base plate located on the top plate, a lifting mechanism on the other side, a placement groove on the top plate, a U-shaped cell filter tube placed in the placement groove, and the bottom of the U-shaped cell filter tube being supported by the lifting mechanism.

[0006] Preferably, the negative pressure extraction mechanism includes a synchronization module, an adapter plate is mounted on the synchronization module, a lead screw module is provided on the adapter plate, a mounting bracket is provided on the lead screw module, and an extraction pin is provided on the top of the mounting bracket.

[0007] Preferably, the lifting mechanism is positioned directly below the U-shaped cell filter tube, with a support groove at its top and the bottom of the U-shaped cell filter tube located within the support groove.

[0008] Preferably, the U-shaped cell filter tube has an inlet and an outlet at its two ends, and a microporous filter membrane is provided inside the tube.

[0009] Preferably, the U-shaped cell filter tube is made of a transparent material.

[0010] The technical effects and advantages of this utility model are as follows:

[0011] The U-shaped tube cell separation device of this invention has the advantages of simple structure, convenient operation, high cell separation efficiency and minimal cell damage. Furthermore, it can be used for the separation of various types of cells through different microporous filter membranes, meeting the needs of fields such as biological experiments and cell therapy. Attached Figure Description

[0012] Figure 1 This is a schematic diagram of the structure of a U-shaped tube cell separation device provided in an embodiment of this application. Figure 1 ;

[0013] Figure 2 This is a schematic diagram of the structure of a U-shaped tube cell separation device provided in an embodiment of this application. Figure 2 ;

[0014] Figure 3 This application provides a U-shaped tube cell separation device. Figure 1 Schematic diagram of the structure at point A;

[0015] Figure 4 This is a schematic diagram of the extraction mechanism in a U-shaped tube cell separation device provided in an embodiment of this application;

[0016] Figure 5 This is a schematic diagram of the lifting mechanism in a U-shaped tube cell separation device provided in an embodiment of this application;

[0017] Figure 6 This is a cross-sectional view of the U-shaped cell filter tube in a U-shaped cell separation device provided in an embodiment of this application.

[0018] In the picture:

[0019] 1. Base plate; 2. Top plate; 3. Negative pressure extraction mechanism; 4. Lifting mechanism; 5. Placement slot; 6. U-shaped cell filter tube; 31. Synchronization module; 32. Adapter plate; 33. Screw module; 34. Mounting bracket; 35. Extraction needle; 41. Support slot; 61. Microporous filter membrane. Detailed Implementation

[0020] The present invention will now be described in further detail with reference to the accompanying drawings and specific embodiments. The embodiments of the present invention are given for illustrative and descriptive purposes only, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those skilled in the art. The embodiments were chosen and described to better illustrate the principles and practical applications of the present invention, and to enable those skilled in the art to understand the present invention and design various embodiments with various modifications suitable for a particular purpose.

[0021] Example

[0022] Please see Figures 1-6 In this embodiment, a U-shaped tube cell separation device is provided, including a base plate 1, a top plate 2 at the upper end of the base plate 1, a negative pressure extraction mechanism 3 on one side of the top plate 2 on the base plate 1, and a lifting mechanism 4 on the other side. A placement groove 5 is opened on the top plate 2, and a U-shaped cell filter tube 6 is placed in the placement groove 5. The bottom of the U-shaped cell filter tube 6 is supported by the lifting mechanism 4.

[0023] In use, the U-shaped cell filter tube 6 containing the cell fluid to be separated is placed into the placement tank 5. Then, the negative pressure extraction mechanism 3 is activated, so that the bottom needle of its extraction needle 35 penetrates into the U-shaped tube for negative pressure extraction. Cell separation is achieved through negative pressure and the microporous filter membrane 61 inside the U-shaped cell filter tube 6. The U-shaped cell filter tube 6 is then lifted from the placement tank 5 by the lifting mechanism 4. Finally, the U-shaped cell filter tube 6 is removed by the clamping mechanism (an external device not shown; the removal of the U-shaped cell filter tube 6 is automated by the external clamping mechanism) for the next step of inspection.

[0024] The negative pressure extraction mechanism 3 includes a synchronization module 31, an adapter plate 32 mounted on the synchronization module 31, a lead screw module 33 mounted on the adapter plate 32, a mounting frame 34 mounted on the lead screw module 33, and an extraction needle 35 mounted on the top of the mounting frame 34. The extraction needle 35 is connected to an external negative pressure device to achieve the negative pressure extraction effect. The synchronization module 31 can drive the adapter plate 32 to move horizontally, while the lead screw module 33 drives the mounting frame 34 to move up and down. The two work together to enable the extraction needle 35 to move horizontally and vertically. As a result, the bottom needle of the extraction needle 35 can move to the outlet end of the U-shaped cell filter tube 6 and be inserted into it to achieve the extraction effect and thus separate the cells.

[0025] The top plate 2 has a placement slot 5 suitable for the vertical insertion of the U-shaped cell filter tube 6, which serves to limit the position of the U-shaped cell filter tube 6. At the same time, it works with the lifting mechanism 4 to stably support the U-shaped cell filter tube 6. The top of the lifting mechanism 4 has a support slot 41, and the bottom of the U-shaped cell filter tube 6 is located in the support slot 41 to maintain the stability of the U-shaped cell filter tube 6. The lifting mechanism 4 is located directly below the U-shaped cell filter tube 6 to control the lifting and lowering of the U-shaped cell filter tube 6, thereby facilitating its removal by the clamping mechanism.

[0026] The U-shaped cell filter tube 6 has an inlet and an outlet at its two ends, respectively. The tube body contains a microporous filter membrane 61 for filtering and separating cells, allowing the cell suspension in the U-shaped cell filter tube 6 to be extracted and separated under specific conditions. By controlling the size of the pores in the microporous filter membrane 61, efficient and gentle cell separation can be achieved. U-shaped cell filter tubes 6 with different pore sizes of microporous filter membranes 61 can be replaced according to different usage scenarios to accommodate cells of different sizes. The U-shaped cell filter tube 6 is made of transparent material, which facilitates observation of the cell separation process.

[0027] The U-shaped tube cell separation device of this invention uses the principle of negative pressure to separate cells by drawing liquid at the outlet end of the U-shaped cell filter tube 6. Cells smaller than the pore size of the microporous filter membrane 61 are extracted. Since most diseased cells are larger than the original cells, they will be blocked by the microporous filter membrane 61 and cannot pass through the microporous filter membrane 61. This allows for further detection of diseased cells.

[0028] The U-shaped tube cell separation device of this invention has the advantages of simple structure, convenient operation, high cell separation efficiency and minimal cell damage. Furthermore, it can be used for the separation of various types of cells through different microporous filter membranes 61, meeting the needs of fields such as biological experiments and cell therapy.

[0029] Obviously, the described embodiments are only a part of the embodiments of this utility model, and not all of them. All other embodiments obtained by those skilled in the art and related fields based on the embodiments of this utility model without creative effort should fall within the protection scope of this utility model. Structures, devices, and operating methods not specifically described and explained in this utility model, unless otherwise specified or limited, shall be implemented according to conventional means in the art.

Claims

1. A U-tube cell separation device, characterized by, Includes a base plate (1), a top plate (2) is provided on the upper end of the base plate (1), a negative pressure extraction mechanism (3) is provided on one side of the base plate (1) located on the top plate (2), and a lifting mechanism (4) is provided on the other side. A placement groove (5) is provided on the top plate (2), and a U-shaped cell filter tube (6) is provided in the placement groove (5). The bottom of the U-shaped cell filter tube (6) is supported by the lifting mechanism (4).

2. A U-shaped tubing cell separation device according to claim 1, wherein, The negative pressure extraction mechanism (3) includes a synchronization module (31), an adapter plate (32) is installed on the synchronization module (31), a lead screw module (33) is provided on the adapter plate (32), a mounting bracket (34) is provided on the lead screw module (33), and an extraction pin (35) is provided on the top of the mounting bracket (34).

3. The U-shaped tubing cell separation device of claim 1, wherein, The lifting mechanism (4) is located directly below the U-shaped cell filter tube (6), and a support groove (41) is provided on its top. The bottom of the U-shaped cell filter tube (6) is located in the support groove (41).

4. A U-shaped tubing cell separation device according to claim 3, wherein, The U-shaped cell filter tube (6) has an inlet and an outlet at its two ends, respectively, and a microporous filter membrane (61) is provided inside the tube.

5. A U-shaped tubing cell separation device according to claim 4, wherein, The U-shaped cell filter tube (6) is made of a transparent material.

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