Chicken liver protein extraction equipment

Abstract

The utility model provides chicken liver protein extraction equipment which comprises a processing tank, and an inclined stirring assembly is arranged in the processing tank; one side of the top end of the processing tank is connected with a raw material tank through a raw material discharging pipe; the raw material tank conveys raw materials into the processing tank through the raw material discharging pipe; one side of the top end of the processing tank further communicates with an additive conveying assembly; the additive conveying assembly comprises a first additive tank, a second additive tank, a third additive tank, an additive discharging pipe, a communicating pipe, a metering pump, a spray head and an inclined connecting pipe. A metering pump is arranged in the additive tank I and is communicated with the spray head through a communicating pipe; an inclined connecting pipe is arranged between the raw material discharging pipe and the additive tank I, and a spray head is mounted in the inclined connecting pipe; and the bottom ends of the additive tank II and the additive tank III are communicated with the processing tank through an additive discharging pipe. The device can reduce the generation of bubbles, is favorable for improving the protein recovery rate and purity, and improves the diffusion speed of a sodium chloride solution.

Description

Technical Field

[0001] This utility model relates to the technical field of chicken liver protein processing equipment, specifically a chicken liver protein extraction device. Background Technology

[0002] Chicken liver is rich in albumin and is therefore often used for protein extraction. Protein extraction processes are mainly divided into three types: acid extraction, alkaline extraction, and enzymatic extraction. All three methods involve salting out. The conventional approach is to add sodium chloride solution to the protein solution to precipitate the protein; subsequently, an enzymatic hydrolysate is added for enzymatic hydrolysis.

[0003] Existing salting-out equipment for extraction, such as the chicken liver protein extraction device disclosed in application number 202022722730.6, aims to solve the problem that the protein solution cannot be stirred and blended evenly during enzymatic hydrolysis, thus affecting the protein extraction concentration. The key technical points are: a chicken liver protein extraction device includes a processing tank, a raw material storage tank, and an enzyme storage tank. A stirring device is installed inside the processing tank. The stirring device includes a motor, a first bevel gear connected to the motor's output shaft, and a second bevel gear meshing with the first bevel gear. A first rotating shaft is fixedly connected to the second bevel gear, and a first rotating disk is fixedly connected to the first rotating shaft. Several first stirring blades arranged in a circular array are fixedly connected to the outer peripheral wall of the first rotating disk. This invention's chicken liver protein extraction device, by incorporating a stirring device, ensures a more uniform enzymatic hydrolysis reaction, thereby guaranteeing the concentration of extracted chicken liver protein.

[0004] However, this type of salting-out extraction equipment has a vertical stirring device inside. When in use, the vertical stirring device generates an axisymmetric vortex, forming a low-pressure central area that continuously entrains gas into the liquid. This easily produces a large number of bubbles, which are then encapsulated and stabilized by chicken liver protein (containing lecithin and other surfactants), forming persistent foam. Furthermore, during vertical stirring, the sodium chloride solution tends to form a high-concentration zone in the center of the tank, causing premature precipitation of local proteins. Undissolved salt is encapsulated by the protein, and directly adding the enzymatic hydrolysate will affect the protein recovery rate. Utility Model Content

[0005] In view of the shortcomings of the existing technology, the purpose of this utility model is to provide a chicken liver protein extraction device that can solve the existing problems.

[0006] To achieve the above objectives, the technical solution of this utility model is as follows:

[0007] This utility model is achieved through the following technical solution: a chicken liver protein extraction device, including a processing tank, wherein an inclined stirring assembly is provided inside the processing tank; one side of the top of the processing tank is connected to a raw material tank through a raw material discharge pipe, and the raw material tank conveys raw materials into the processing tank through the raw material discharge pipe;

[0008] The top of the processing tank is also connected to an additive delivery assembly; the additive delivery assembly includes additive tank one, additive tank two, additive tank three, additive discharge pipe, connecting pipe, metering pump, nozzle, and inclined pipe; the additive tank one is equipped with a metering pump, which is connected to the nozzle through the connecting pipe; an inclined pipe is provided between the raw material discharge pipe and the additive tank one, and the nozzle is installed in the inclined pipe; the bottom ends of additive tank two and additive tank three are connected to the processing tank through the additive discharge pipe.

[0009] Furthermore, the bottom cross-section of the inclined pipe adopts a triangular structure, and the inclined pipe is inclined towards the bottom; the additive delivery assembly also includes a scale line, which is set on the side of the additive tank one, additive tank two and additive tank three.

[0010] Furthermore, both the raw material discharge pipe and the additive discharge pipe are equipped with switch valves on their sides.

[0011] Furthermore, the stirring assembly includes a stirring motor, stirring blades, a stirring shaft, and a stirring rod; the stirring shaft is rotatably connected to the processing tank, and multiple stirring blades are provided on the side of the stirring shaft; the stirring blades on the same side are connected to each other by a stirring rod, and the stirring motor is connected to the side of the stirring shaft.

[0012] Furthermore, the processing tank adopts a spherical structure, and the edges of the stirring blades adopt an arc-shaped structure.

[0013] Furthermore, a cleaning connection pipe is connected to the side of the processing tank, and a switch valve is provided on the side of the cleaning connection pipe.

[0014] Furthermore, the bottom of the processing tank is provided with a support, and the bottom of the processing tank is connected to a discharge pipe, in which a switch valve is installed.

[0015] Compared with the prior art, the beneficial effects of this utility model include:

[0016] This invention relates to a chicken liver protein extraction device, which features an inclined stirring assembly. During salting out, this assembly disrupts the symmetry of the flow field, preventing the stable formation of vortices and reducing bubble generation. Furthermore, the shear force generated by the inclined stirring blades is angled towards the liquid surface, pushing the small number of bubbles towards the container wall rather than into the deeper parts of the liquid, thus improving protein recovery rate and purity. Simultaneously, the inclined stirring generates three-dimensional turbulence (compared to the two-dimensional circulation of vertical stirring), increasing the diffusion rate of the sodium chloride solution.

[0017] Furthermore, this invention features multiple additive tanks, allowing for the addition of sodium chloride solutions of varying or equal concentrations in batches for salting out, as needed. In the salting-out process of chicken liver protein extraction, adding salt solutions of different concentrations stepwise is a key strategy for achieving efficient separation and purification of the target protein. Different salt concentrations alter the ionic strength and protein solubility of the solution, enabling targeted separation of various components. Therefore, the multiple additive tanks in this invention provide a more convenient and intuitive way to add sodium chloride solution multiple times. Attached Figure Description

[0018] The disclosure of this utility model is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this utility model. In the drawings, the same reference numerals are used to refer to the same parts. Wherein:

[0019] Figure 1 This is a schematic diagram of the overall structure of a chicken liver protein extraction device according to the present invention;

[0020] Figure 2 This is an embodiment of the present utility model. Figure 1 A magnified structural diagram of point A in the middle.

[0021] The diagram is labeled as follows: 1. Feed pipe; 2. Support; 3. Processing tank; 31. Insulation filling layer; 4. Mixing assembly; 41. Mixing motor; 42. Mixing blades; 43. Mixing shaft; 44. Mixing rod; 5. Cleaning connection pipe; 6. Switch valve; 7. Raw material tank; 71. Raw material discharge pipe; 8. Additive conveying assembly; 81. Additive tank one; 82. Additive tank two; 83. Additive tank three; 84. Scale line; 85. Additive discharge pipe; 86. Connecting pipe; 87. Metering pump; 88. Nozzle; 89. Angled pipe. Detailed Implementation

[0022] It is readily understood that, based on the technical solution of this utility model, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of this utility model. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative descriptions of the technical solution of this utility model and should not be considered as the entirety of this utility model or as limitations or restrictions on the technical solution of this utility model.

[0023] This utility model provides a chicken liver protein extraction device, such as... Figure 1As shown, the processing tank 3 has a sandwich structure, with an insulating filling layer 31 filling the sandwich structure. An inclined stirring assembly 4 is installed inside the processing tank 3. Specifically, the stirring assembly 4 includes a stirring motor 41, stirring blades 42, a stirring shaft 43, and stirring rods 44. The stirring shaft 43 is rotatably connected to the processing tank 3, and multiple stirring blades 42 are arranged on its side. The stirring blades 42 on the same side are connected by stirring rods 44, and the stirring motor 41 is connected to the side of the stirring shaft 43. The processing tank 3 has a spherical structure, and the edges of the stirring blades 42 have an arc-shaped structure. The vertical stirring assembly generates axisymmetric vortices during stirring, forming a low-pressure central region that continuously entrains gas into the liquid, thus easily generating bubbles. This application features an inclined stirring component 4 with an inclination angle (25°-45° with respect to the vertical direction), which can disrupt the symmetry of the flow field and prevent vortices from forming stably. Furthermore, the shear force generated by the inclined stirring blades 42 is at an angle to the liquid surface, pushing bubbles toward the container wall rather than the depth of the liquid, which helps to improve protein recovery rate and purity.

[0024] The top of the processing tank 3 is connected to the raw material tank 7 via a raw material discharge pipe 71. The raw material tank 7 conveys raw materials into the processing tank 3 through the raw material discharge pipe 71. The raw materials fall into the processing tank under the action of gravity.

[0025] The top of the processing tank 3 is also connected to an additive delivery assembly 8; the additive delivery assembly 8 includes an additive tank 1 81, an additive tank 2 82, an additive tank 3 83, an additive discharge pipe 85, a connecting pipe 86, a metering pump 87, a nozzle 88, and an inclined pipe 89; this application uses three additive tanks as an example for illustration, but it is not limited to this, and the number of additive tanks is within the scope of protection of this application, such as... Figure 2As shown, the additive tank 1 81 is equipped with a metering pump 87, and the output end of the metering pump 87 is connected to a nozzle 88 through a connecting pipe 86. A slanted pipe 89 is provided between the raw material discharge pipe 71 and the additive tank 1 81, and the nozzle 88 is installed inside the slanted pipe 89. The nozzle 88 is used to spray sodium chloride solution onto the raw material falling into the raw material discharge pipe 71 to achieve preliminary mixing. The bottom ends of the additive tanks 2 82 and 3 83 are connected to the processing tank 3 through an additive discharge pipe 85. The bottom cross-section of the slanted pipe 89 has a triangular structure, and the slanted pipe 89 is inclined towards the bottom. The additive delivery assembly 8 also includes a scale line 84, which is set on the side of the additive tanks 1 81, 2 82, and 3 83, and is used to record the amount sprayed. To improve spraying efficiency and accuracy, the nozzle 88 can be equipped with an anti-crystallization design, such as an electrically heated sleeve on the outer wall of the nozzle to maintain a temperature of 30-50℃ to prevent high-concentration NaCl crystallization. The heating sleeve is made of 316L stainless steel with a power density ≤10W / cm³. 2 A combination of nozzles and metering pumps can also be installed in additive tanks 2 (82) and 3 (83).

[0026] This application sets up three additive tanks, each containing sodium chloride solutions of the same or different concentrations. During the extraction and salting-out operation, the sodium chloride solutions can be added in batches, preferably with different concentrations. For example, step 1: low-salt precipitation (exemplary, 0.5-1M NaCl), target: removal of impurity proteins (such as hemoglobin, membrane proteins); function: precipitating acidic impurity proteins with low isoelectric points (pI 4.5-5.5), retaining the target protein (such as albumin, enzymes) in the supernatant; pH 5.0-6.0 (close to the pI of impurity proteins), temperature 4-10℃ (to reduce denaturation). Step 2: medium-salt precipitation (exemplary, 1.5-2M NaCl), target: precipitation of the main target protein (such as chicken liver albumin), function: disrupting the hydration layer of the target protein to neutralize its surface charge (albumin pI≈4.7-5.2). Step 3: High-salt precipitation (exemplary, 2.0-3.0M NaCl), Target: Precipitate highly hydrophobic proteins (such as certain enzymes or membrane-bound proteins), Function: Completely disrupt hydrophobic interactions, used for the extraction of extremely stable proteins (such as superoxide dismutase). Therefore, in the chicken liver protein extraction process, adding salt solutions of different concentrations in stages is a key strategy for achieving efficient separation and purification of target proteins. Different salt concentrations, by altering the ionic strength and protein solubility of the solution, allow for the targeted separation of various components.

[0027] Both the raw material discharge pipe 71 and the additive discharge pipe 85 are equipped with switch valves 6 on their sides. These valves are used to control whether materials are added. In this application, the switch valve 6 is a manual or electric control valve, which is prior art.

[0028] The processing tank 3 is connected to a cleaning connection pipe 5 on its side. The cleaning connection pipe 5 can be connected to an external cleaning water pipe to add water for cleaning into the processing tank 3. The side of the cleaning connection pipe 5 is equipped with a switch valve 6.

[0029] The bottom of the processing tank 3 is provided with a support 2, and the bottom of the processing tank 3 is connected to the feed pipe 1. A switch valve 6 is installed in the feed pipe 1 to take out the solution after salting out. After obtaining the extract, it is determined whether the salt concentration of the solution needs to be reduced according to the needs. If necessary, an enzymatic hydrolysate is added for enzymatic extraction.

[0030] In use, this application involves adding the required sodium chloride solution in batches to additive tank 1 (81), additive tank 2 (82), and additive tank 3 (83) as needed. The raw material tank 7 contains the chicken liver protein solution to be processed. During salting out, the metering pump 87 and the switch valve 6 on the side of the raw material outlet pipe 71 are opened, and the stirring motor 41 is turned on. The chicken liver protein solution and the sodium chloride solution sprayed from the nozzle 88 then enter the processing tank 3 and are stirred using the stirring assembly 4. After stirring for a period of time, the switch valve 6 at the bottom of additive tank 2 (82) is opened, and sodium chloride solution is added a second time while stirring. Then, the sodium chloride solution from additive tank 3 (83) is added and stirred for salting out. After salting out, the solution can be removed through the discharge pipe 1 and desalted or diluted as needed before adding enzymatic hydrolysate for enzymatic hydrolysis, completing the entire extraction process. This application allows for direct desalting or dilution within the processing tank 3, and enzymatic hydrolysate can be added directly through the cleaning connecting pipe 5.

[0031] In the description of this utility model, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc., indicating the orientation or positional relationship are based on the orientation or positional relationship shown in the accompanying drawings, and are only for the convenience of describing this utility model and simplifying the description, and are not intended to indicate or imply that the device or component referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of this utility model.

[0032] In the description of this patent, it should be noted that, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," and "setting" should be interpreted broadly. For example, they can refer to a fixed connection or setting, a detachable connection or setting, or an integral connection or setting. Those skilled in the art can understand the specific meaning of the above terms in this patent according to the specific circumstances.

[0033] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of this utility model, "several" means two or more, unless otherwise explicitly specified.

[0034] The technical scope of this utility model is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this utility model, and all such modifications and variations should fall within the protection scope of this utility model.

Claims

1. A chicken liver protein extraction device, characterized in that: The equipment includes a processing tank (3), and an inclined stirring assembly (4) is provided inside the processing tank (3); one side of the top of the processing tank (3) is connected to a raw material tank (7) through a raw material discharge pipe (71), and the raw material tank (7) conveys raw materials into the processing tank (3) through the raw material discharge pipe (71); The top of the processing tank (3) is also connected to an additive delivery assembly (8); the additive delivery assembly (8) includes an additive tank one (81), an additive tank two (82), an additive tank three (83), an additive discharge pipe (85), a connecting pipe (86), a metering pump (87), a nozzle (88), and an inclined pipe (89); the additive tank one (81) is equipped with a metering pump (87), which is connected to the nozzle (88) through the connecting pipe (86); an inclined pipe (89) is provided between the raw material discharge pipe (71) and the additive tank one (81), and the nozzle (88) is installed in the inclined pipe (89); the bottom ends of the additive tank two (82) and the additive tank three (83) are connected to the processing tank (3) through the additive discharge pipe (85).

2. The chicken liver protein extraction apparatus according to claim 1, characterized in that: The bottom section of the inclined pipe (89) adopts a triangular structure, and the inclined pipe (89) is inclined to the bottom; the additive delivery assembly (8) also includes a scale line (84), which is set on the side of the additive tank one (81), additive tank two (82) and additive tank three (83).

3. The chicken liver protein extraction apparatus according to claim 1, wherein: Both the raw material discharge pipe (71) and the additive discharge pipe (85) are equipped with switch valves (6) on their sides.

4. The chicken liver protein extraction apparatus according to claim 1, characterized in that: The stirring assembly (4) includes a stirring motor (41), stirring blades (42), stirring shaft (43), and stirring rod (44); the stirring shaft (43) is rotatably connected to the processing tank (3), and a plurality of stirring blades (42) are provided on the side of the stirring shaft (43); the stirring blades (42) on the same side are connected to each other by stirring rod (44), and the stirring motor (41) is connected to the side of the stirring shaft (43).

5. A chicken liver protein extraction apparatus according to claim 4, wherein: The processing tank (3) has a spherical structure, and the edge of the stirring blade (42) has an arc-shaped structure.

6. The chicken liver protein extraction apparatus according to claim 1, wherein: The processing tank (3) is connected to a cleaning connection pipe (5) on its side, and the cleaning connection pipe (5) is provided with a switch valve (6) on its side.

7. The chicken liver protein extraction apparatus according to claim 1, wherein: The bottom end of the processing tank (3) is provided with a bracket (2), and the bottom end of the processing tank (3) is connected to the feeding pipe (1), and a switch valve (6) is installed in the feeding pipe (1).

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