Phage antibody separation and purification device

Abstract

The utility model relates to the technical field of bacteriophage culture and separation, in particular to a bacteriophage antibody separation and purification device, which is characterized in that when the bacteriophage antibody separation and purification device is used, bacteriophage and mixed liquid are poured into a stirring tank, and a stirring component is started, so that the stirring component stirs the bacteriophage and the mixed liquid in the horizontal direction and in the vertical direction simultaneously; when the inner side wall of the stirring tank needs to be cleaned, the washing assembly is operated to wash the inner side wall of the stirring tank, after stirring is completed, mixed liquid is discharged through the liquid discharging assembly, and convenience is improved; comprising a stirring tank and supporting legs, the multiple sets of supporting legs are installed at the bottom end of the stirring tank, the stirring device further comprises a liquid discharging assembly, a stirring assembly and a flushing assembly, the stirring assembly is arranged in the stirring tank, the flushing assembly is installed on the stirring tank, and the liquid discharging assembly is installed on the stirring tank.

Description

Technical Field

[0001] This utility model relates to the technical field of phage culture and isolation, and in particular to a phage antibody isolation and purification device. Background Technology

[0002] Bacteriophages are viruses that infect bacteria and also the genetic material that imparts biological traits to the host bacteria. Bacteriophages are the most common and widely distributed group of viruses, and they are usually found in places full of bacterial communities. Stirring is usually required when isolating and purifying bacteriophage antibodies.

[0003] In the prior art, patent document CN220376654U discloses a phage isolation device for phage culture, including a stirring tank. The top of the stirring tank is equipped with a top cover, and a motor is installed at the bottom of one side of the stirring tank. The output end of the motor extends into the interior of the stirring tank, and the output of the motor is rotatably connected to a transmission rod. A second bevel gear is installed at one end of the transmission rod, and a first bevel gear is installed at the bottom inner side of the stirring tank. The first bevel gear and the second bevel gear are meshed and connected. A stirring shaft is installed at the center of the top surface of the first bevel gear, and stirring blades are fixed at equal intervals on the surface of the stirring shaft. Functional components are provided at the bottom inner side of the stirring tank.

[0004] During use, it was found that the stirring direction of the above device was relatively uniform, resulting in poor stirring uniformity. Utility Model Content

[0005] To solve the above-mentioned technical problems, this utility model provides a phage antibody separation and purification device.

[0006] This invention relates to a phage antibody separation and purification device, comprising a stirring tank and supporting legs. Multiple sets of supporting legs are installed at the bottom of the stirring tank. The device also includes a drainage component, a stirring component, and a rinsing component. The stirring component is installed inside the stirring tank, and a rinsing component and a drainage component are installed on the stirring tank. In use, phage and a mixture are poured into the stirring tank, and the stirring component is activated. This allows the stirring component to simultaneously stir the phage and mixture horizontally and vertically, improving stirring efficiency. When cleaning the inner wall of the stirring tank is required, the rinsing component is used to rinse the inner wall. After stirring is complete, the mixture is drained through the drainage component, improving convenience.

[0007] Preferably, the stirring assembly includes a support frame, a motor, a rotating shaft, stirring blades, support rods, and guide tubes. The motor is mounted on the top of the stirring tank via the support frame. A rotating shaft is located at the motor output end, with its bottom end extending into the stirring tank. Multiple sets of stirring blades are mounted on the rotating shaft inside the stirring tank. Multiple sets of support rods are mounted on the rotating shaft, and each set of support rods is equipped with a set of guide tubes. The multiple sets of guide tubes are arranged in a circumferential array around the rotating shaft. When it is necessary to stir the phage and the mixture, the motor is started, causing the rotating shaft to drive the multiple sets of stirring blades to stir the phage and the mixture horizontally. At the same time, the two sets of support rods drive the two sets of guide tubes to rotate rapidly. The phage and the mixture enter the guide tube through the horizontal end of the guide tube and, under the action of inertia, are discharged to the upper part of the stirring tank through the other end of the guide tube, thereby achieving vertical mixing of the phage and the mixture and improving stirring efficiency.

[0008] Preferably, the flushing assembly includes a water pump, a mounting base, an inlet pipe, an outlet pipe, an annular pipe, high-pressure nozzles, and a check valve. The water pump is mounted on the mixing tank via the mounting base. An annular pipe is installed at the top of the mixing tank, and multiple sets of high-pressure nozzles are installed at the bottom of the annular pipe. An inlet pipe is installed at the input end of the water pump, and an outlet pipe is installed at the output end of the water pump. The output end of the outlet pipe is connected to the input end of the annular pipe, and a check valve is installed on the outlet pipe. When it is necessary to flush the inner wall of the mixing tank, the water pump is started, so that water enters the annular pipe through the inlet and outlet pipes. The water entering the annular pipe flushes the inner wall of the mixing tank through the multiple sets of high-pressure nozzles, thereby improving the cleaning efficiency.

[0009] Preferably, the drainage assembly includes a drain pipe and a drain valve. The drainage pipe is installed on the mixing tank, and the drain valve is installed on the drainage pipe. After mixing is completed, the drain valve is opened, so that the mixture is discharged through the drain pipe, improving convenience.

[0010] Preferably, it also includes an observation window, which is provided on the mixing tank; the staff can observe the liquid level of the mixture inside the mixing tank to improve convenience.

[0011] Preferably, it also includes a base, with a base installed at the bottom of each set of support legs; the base and support legs work together to provide stable support for the mixing tank and improve stability.

[0012] Preferably, it also includes round holes, with multiple sets of round holes provided on each set of stirring blades; the stirring blades and multiple sets of round holes work together to mix the bacteriophage and the mixture in a horizontal direction, thereby improving the stirring efficiency.

[0013] Compared with the prior art, the beneficial effects of this utility model are as follows: When in use, the bacteriophage and the mixture are poured into the mixing tank, and the stirring component is started, so that the stirring component can stir the bacteriophage and the mixture in the horizontal direction while stirring in the vertical direction, thereby improving the stirring efficiency. When it is necessary to clean the inner wall of the mixing tank, the flushing component can be operated to flush the inner wall of the mixing tank. After the stirring is completed, the mixture is discharged through the draining component, which improves convenience. Attached Figure Description

[0014] Figure 1 This is a schematic diagram of the isometric structure of this utility model;

[0015] Figure 2 This is a cross-sectional structural schematic diagram of the present invention;

[0016] Figure 3 It is an enlarged structural diagram of structures such as ring pipes and water pumps;

[0017] Figure 4 It is an enlarged structural diagram of the rotating shaft and the guide tube.

[0018] The following are labels in the attached diagram: 1. Mixing tank; 2. Support leg; 3. Support frame; 4. Motor; 5. Rotating shaft; 6. Mixing blade; 7. Support rod; 8. Guide pipe; 9. Water pump; 10. Mounting base; 11. Inlet pipe; 12. Outlet pipe; 13. Annular pipe; 14. High-pressure nozzle; 15. Check valve; 16. Drain pipe; 17. Drain valve; 18. Observation window; 19. Base; 20. Round hole. Detailed Implementation

[0019] To facilitate understanding of this utility model, a more complete description will be given below with reference to the accompanying drawings. This utility model can be implemented in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided to make the disclosure of this utility model more thorough and complete.

[0020] Example 1

[0021] like Figures 1 to 3 As shown, the present invention provides a phage antibody separation and purification device, which includes a stirring tank 1 and support legs 2. The bottom of the stirring tank 1 is equipped with multiple sets of support legs 2. The device also includes a drainage component, a stirring component, and a rinsing component. The stirring tank 1 is equipped with a stirring component inside, a rinsing component is installed on the stirring tank 1, and a drainage component is installed on the stirring tank 1.

[0022] like Figure 1 , Figure 2 and Figure 4As shown, the stirring assembly includes a support frame 3, a motor 4, a rotating shaft 5, stirring blades 6, support rods 7, and guide pipes 8. The motor 4 is mounted on the top of the stirring tank 1 via the support frame 3. The output end of the motor 4 is provided with a rotating shaft 5, and the bottom end of the rotating shaft 5 extends into the interior of the stirring tank 1. Multiple sets of stirring blades 6 are mounted on the rotating shaft 5 inside the stirring tank 1. Multiple sets of support rods 7 are provided on the rotating shaft 5, and a set of guide pipes 8 are installed on each set of support rods 7. The multiple sets of guide pipes 8 are arranged in a circular array around the rotating shaft 5.

[0023] In this embodiment, during use, the bacteriophage and the mixture are poured into the mixing tank 1, and the motor 4 is started, so that the rotating shaft 5 drives multiple sets of stirring blades 6 to stir the bacteriophage and the mixture horizontally. At the same time, two sets of support rods 7 drive two sets of guide tubes 8 to rotate rapidly. The bacteriophage and the mixture enter the guide tube 8 through the horizontal end of the guide tube 8 and are discharged to the upper part of the mixing tank 1 through the other end of the guide tube 8 under the action of inertia. This achieves vertical mixing of the bacteriophage and the mixture, improving the stirring efficiency. When it is necessary to clean the inner wall of the mixing tank 1, the flushing component can be operated to flush the inner wall of the mixing tank 1. After the stirring is completed, the mixture is discharged through the drain component, improving convenience.

[0024] Example 2

[0025] like Figures 1 to 3 As shown, the present invention provides a phage antibody separation and purification device, which includes a stirring tank 1 and support legs 2. The bottom of the stirring tank 1 is equipped with multiple sets of support legs 2. The device also includes a drainage component, a stirring component, and a rinsing component. The stirring tank 1 is equipped with a stirring component inside, a rinsing component is installed on the stirring tank 1, and a drainage component is installed on the stirring tank 1.

[0026] like Figure 1 , Figure 2 and Figure 4 As shown, the stirring assembly includes a support frame 3, a motor 4, a rotating shaft 5, stirring blades 6, support rods 7, and guide pipes 8. The motor 4 is mounted on the top of the stirring tank 1 via the support frame 3. The output end of the motor 4 is provided with a rotating shaft 5, and the bottom end of the rotating shaft 5 extends into the interior of the stirring tank 1. Multiple sets of stirring blades 6 are mounted on the rotating shaft 5 inside the stirring tank 1. Multiple sets of support rods 7 are provided on the rotating shaft 5, and a set of guide pipes 8 are installed on each set of support rods 7. The multiple sets of guide pipes 8 are arranged in a circular array around the rotating shaft 5.

[0027] like Figure 3As shown, the flushing assembly includes a water pump 9, a mounting base 10, an inlet pipe 11, an outlet pipe 12, an annular pipe 13, a high-pressure nozzle 14, and a check valve 15. The water pump 9 is mounted on the mixing tank 1 via the mounting base 10. An annular pipe 13 is provided at the top of the mixing tank 1, and multiple sets of high-pressure nozzles 14 are provided at the bottom of the annular pipe 13. The water pump 9 has an inlet pipe 11 at its input end and an outlet pipe 12 at its output end. The output end of the outlet pipe 12 is connected to the input end of the annular pipe 13. A check valve 15 is installed on the outlet pipe 12.

[0028] The drainage assembly includes a drain pipe 16 and a drain valve 17. The drain pipe 16 is installed on the mixing tank 1, and the drain valve 17 is installed on the drain pipe 16.

[0029] It also includes an observation window 18, which is provided on the mixing tank 1;

[0030] It also includes a base 19, with a base 19 installed at the bottom of each set of support legs 2;

[0031] It also includes round holes 20, with multiple sets of round holes 20 provided on each set of stirring blades 6.

[0032] In this embodiment, during use, the bacteriophage and the mixture are poured into the mixing tank 1, and the motor 4 is started, so that the rotating shaft 5 drives multiple sets of stirring blades 6 to stir the bacteriophage and the mixture horizontally. At the same time, two sets of support rods 7 drive two sets of guide pipes 8 to rotate rapidly. The bacteriophage and the mixture enter the guide pipe 8 through the horizontal end of the guide pipe 8 and are discharged to the upper part of the mixing tank 1 through the other end of the guide pipe 8 under the action of inertia. This achieves vertical mixing of the bacteriophage and the mixture, improving the stirring efficiency. When it is necessary to flush the inner wall of the mixing tank 1, the water pump 9 is started, so that water enters the annular pipe 13 through the inlet pipe 11 and the outlet pipe 12. The water entering the annular pipe 13 flushes the inner wall of the mixing tank 1 through multiple sets of high-pressure nozzles 14. After the stirring is completed, the drain valve 17 is opened, so that the mixture is discharged through the drain pipe 16.

[0033] The motor 4 and water pump 9 of the phage antibody separation and purification device of this utility model are commercially available. Technical personnel in this industry only need to install and operate them according to the accompanying instruction manual, without requiring any creative work from those skilled in the art.

[0034] The above description is only a preferred embodiment of the present utility model. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present utility model, and these improvements and modifications should also be considered within the protection scope of the present utility model.

Claims

1. A phage antibody separation and purification device, comprising a stirred tank (1) and supporting legs (2), wherein multiple sets of supporting legs (2) are installed at the bottom of the stirred tank (1), characterized in that, It also includes a drain assembly, a stirring assembly and a flushing assembly. The stirring tank (1) is equipped with a stirring assembly, a flushing assembly and a drain assembly. The stirring assembly includes a support frame (3), a motor (4), a rotating shaft (5), stirring blades (6), support rods (7), and guide pipes (8). The motor (4) is mounted on the top of the stirring tank (1) via the support frame (3). The output end of the motor (4) is provided with a rotating shaft (5). The bottom end of the rotating shaft (5) extends into the interior of the stirring tank (1). Multiple sets of stirring blades (6) are mounted on the rotating shaft (5) inside the stirring tank (1). Multiple sets of support rods (7) are provided on the rotating shaft (5). Each set of support rods (7) is equipped with a set of guide pipes (8). The multiple sets of guide pipes (8) are arranged in a circular array around the rotating shaft (5).

2. The phage antibody separation and purification device as described in claim 1, characterized in that, The flushing assembly includes a water pump (9), a mounting base (10), an inlet pipe (11), an outlet pipe (12), an annular pipe (13), a high-pressure nozzle (14), and a check valve (15). The water pump (9) is mounted on the mixing tank (1) via the mounting base (10). An annular pipe (13) is provided at the top inside the mixing tank (1). Multiple sets of high-pressure nozzles (14) are provided at the bottom of the annular pipe (13). An inlet pipe (11) is provided at the input end of the water pump (9), and an outlet pipe (12) is provided at the output end of the water pump (9). The output end of the outlet pipe (12) is connected to the input end of the annular pipe (13). A check valve (15) is installed on the outlet pipe (12).

3. The phage antibody separation and purification device as described in claim 1, characterized in that, The drainage assembly includes a drain pipe (16) and a drain valve (17). The drain pipe (16) is installed on the mixing tank (1), and the drain valve (17) is installed on the drain pipe (16).

4. The phage antibody separation and purification device as described in claim 1, characterized in that, It also includes an observation window (18), which is provided on the mixing tank (1).

5. The phage antibody separation and purification device as described in claim 1, characterized in that, It also includes a base (19), with a base (19) installed at the bottom of each set of support legs (2).

6. The phage antibody separation and purification device as described in claim 1, characterized in that, It also includes round holes (20), with multiple sets of round holes (20) provided on each set of stirring blades (6).

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