Ginger exosome extraction system

Abstract

The utility model discloses a fresh ginger exosome extraction system which comprises a fresh ginger tissue pretreatment device, a gingerol pretreatment device, a first filtering device, a second filtering device, a third filtering device and a waste liquid tank, the filtrate end of the first filtering device is connected to the gingerol pretreatment device, and the output end of the gingerol pretreatment device is connected to the second filtering device; the filtrate end of the second filtering device is connected into the third filtering device; a concentrated solution collecting device is arranged at the filtrate end of the third filtering device; through cooperation of all the devices, ordered treatment of the fresh ginger exosome is achieved, operation such as extraction, filtration and detection of the fresh ginger exosome can be effectively carried out, a stable treatment system is provided for related research and application of the fresh ginger exosome, and the structural design is reasonable.

Description

A ginger exosome extraction system Technical Field

[0001] This utility model relates to the field of exosome extraction technology, specifically a ginger exosome extraction system. Background Technology

[0002] Exosomes are extracellular vesicles with a diameter between 30 and 150 nanometers. They are formed from the endosome membrane within the cell through inward budding and eventually fuse with the cell membrane before being secreted into the extracellular space. Their structural characteristic is a closed lipid bilayer membrane structure. Ginger exosomes, with their natural bioactivity and nanoscale size advantage, can efficiently penetrate periodontal tissues, regulate the inflammatory microenvironment, and accelerate tissue regeneration. They have shown great application potential in disease diagnosis and detection, disease treatment, and drug delivery, making them a newly emerging and popular research area in recent years.

[0003] In the current field of post-periodontal surgery restoration, traditional treatment methods suffer from poor permeability, limited tissue regeneration capacity, and low safety. Ginger exosomes, with their nanoscale size and natural bioactivity, can efficiently penetrate periodontal tissue, precisely regulate the inflammatory response, and promote tissue regeneration. By sustaining the release of exosome active ingredients, the duration of action is significantly prolonged while maintaining the exosome's bioactivity, providing a superior treatment option for post-periodontal surgery restoration.

[0004] However, existing methods for extracting ginger exosomes, such as the one disclosed in patent number "CN116024157A," face numerous challenges in extraction technology due to the highly complex composition of ginger exosomes within cell culture media. These challenges include poor coordination between different steps, low extraction efficiency, and difficulty in precisely controlling the extraction state. This severely limits the widespread clinical application of ginger exosomes. Given the urgent need for efficient, large-scale extraction of ginger exosomes, there is a pressing need to develop a highly efficient and standardized exosome extraction system to address the key bottlenecks in existing technologies. Summary of the Invention

[0005] The purpose of this invention is to provide a ginger exosome extraction system to solve the problems of incomplete tissue disruption, gingerol interference with separation efficiency, and the difficulty of large-scale production using traditional methods in the extraction of plant exosomes.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a ginger exosome extraction system, comprising a ginger tissue pretreatment device, a first filtration device, a ginger extract storage tank, a gingerol pretreatment device, a second filtration device, a transfer tank, and a third filtration device. The output end of the ginger tissue pretreatment device is connected to the first filtration device, the filtrate end of the first filtration device is connected to the ginger extract storage tank, the outlet end of the ginger extract storage tank is connected to the gingerol pretreatment device, and the output end of the gingerol pretreatment device is connected to the second filtration device. The filtrate end of the second filtration device is connected to the transfer tank, and the output end of the transfer tank is connected to the third filtration device. The filtrate end of the third filtration device is equipped with a concentrate collection device.

[0007] It also includes a slow-release liquid filling tank and a particle size analyzer. The output end of the slow-release liquid filling tank is connected to a ginger extract storage tank, and the particle size analyzer is connected to the filtrate end of the third filtration device.

[0008] The second filtration device is equipped with a first switching valve at the filtrate end. One end of the first switching valve is connected to the ginger extract storage tank, and the other end is connected to the third filtration device.

[0009] Preferably, the filtrate end of the third filtration device is provided with a second switching valve, one end of which is connected to a concentrate collection device and the other end is connected to a transfer tank.

[0010] The ginger tissue pretreatment device is used to break down ginger tissue, and the first filtration device is used to remove fibrous impurities with a particle size greater than 50 μm. The input and output ends of the second filtration device form a circulation loop with the ginger extract storage tank through a first switching valve. The input and output ends of the third filtration device form a circulation loop with the transfer tank through a second switching valve.

[0011] Preferably, a first hybrid power pump is provided between the ginger extract storage tank and the gingerol pretreatment device, with the inlet end of the first hybrid power pump connected to the ginger extract storage tank and the outlet end of the first hybrid power pump connected to the gingerol pretreatment device.

[0012] Preferably, a second hybrid power pump is provided between the transfer tank and the third filtration device, with the inlet end of the second hybrid power pump connected to the transfer tank and the outlet end of the second hybrid power pump connected to the second filtration device.

[0013] Preferably, it also includes a waste liquid tank, and the discharge end of the concentrate collection device is connected to the waste liquid tank.

[0014] Preferably, the filtration aperture of the first filtration device is in the range of 5–20 μm, used to remove ginger fiber and large particulate impurities.

[0015] Preferably, the pore size of the second filter device is in the range of 0.1–5 μm, further removing residual particles.

[0016] Preferably, the pore size of the third filtration device is in the range of 30–150 nm, used to retain target exosomes and remove small molecule impurity proteins.

[0017] Preferably, the input or output end of the ginger tissue pretreatment device, the first filtration device, the ginger extract storage tank, the gingerol pretreatment device, the second filtration device, the transfer tank, and the third filtration device is equipped with a one-way valve to prevent liquid backflow.

[0018] Preferably, the ginger extract storage tank and the transfer tank are equipped with pressure sensors and liquid level sensors to achieve accurate monitoring of volume and pressure.

[0019] Preferably, both the second and third filtration devices employ tangential flow filtration to reduce membrane clogging.

[0020] Compared with the prior art, the beneficial effects of this utility model are:

[0021] This invention relates to a ginger exosome extraction system. First, it achieves efficient separation of gingerol through a ginger tissue pretreatment device, avoiding interference with exosome activity. Second, it employs a three-stage filtration system of micro-sieve-microfiltration-ultrafiltration, which is adapted to the high viscosity characteristics of plant tissues and provides excellent separation results. Third, it operates in a fully enclosed manner, conforming to GMP standards and suitable for large-scale and continuous production. Fourth, this system reduces costs by more than 60% compared to existing ultracentrifugal extraction devices. Attached Figure Description

[0022] Figure 1 is a flowchart of the extraction process of this utility model, wherein:

[0023] Component names and serial numbers: 1. Ginger tissue pretreatment device; 2. First filtration device; 3. Ginger extract temporary storage tank; 4. Slow-release solution filling tank; 5. First hybrid pump; 6. Gingerol pretreatment device; 7. Second filtration device; 8. Transfer tank; 9. Second hybrid pump; 10. Third filtration device; 11. Particle size analyzer; 12. Concentrate collection device; 13. Waste liquid tank. Detailed Implementation

[0024] The specific embodiments of this utility model are described in detail below with reference to the accompanying drawings.

[0025] Please refer to Figure 1. This utility model provides the following technical solution: a ginger exosome extraction system, including a ginger tissue pretreatment device 1, a first filtration device 2, a ginger extract storage tank 3, a gingerol pretreatment device 6, a second filtration device 7, a transfer tank 8, and a third filtration device 10. The output end of the ginger tissue pretreatment device 1 is connected to the first filtration device 2, and the filtrate end of the first filtration device 2 is connected to the ginger extract storage tank 3 to achieve storage of ginger samples after preliminary filtration. The outlet end of the ginger extract storage tank 3 is connected to the gingerol pretreatment device 6, and the output end of the gingerol pretreatment device 6 is connected to the second filtration device 7. The filtrate end of the second filtration device 7 is connected to the transfer tank 8, and the output end of the transfer tank 8 is connected to the third filtration device 10. The filtrate end of the third filtration device 10 is provided with a concentrate collection device 12 for collecting concentrate containing ginger exosomes.

[0026] Specifically, it also includes a slow-release solution filling tank 4, the output end of which is connected to a ginger extract storage tank 3. When the volume of the raw material inside the ginger extract storage tank 3 is one-tenth of the original volume, slow-release solution is added to it. This is used to dilute the ginger sample when necessary, so as to maintain the stability and extraction efficiency of the extraction system. In this embodiment, the slow-release solution is PBS phosphate buffer with a concentration of 0.01M.

[0027] Specifically, the second filtration device 7 is equipped with a first switching valve at the filtrate end. One end of the first switching valve is connected to the ginger extract storage tank 3, and the other end is connected to the third filtration device 10. In this embodiment, the number of cycles is 3-4.

[0028] Specifically, it also includes a particle size analyzer 11, which is connected to the filtrate end of the third filtration device 10. The filtrate end of the third filtration device 10 is equipped with a second switching valve, one end of which is connected to the concentrate collection device 12, and the other end is connected to the transfer tank 8. The particle size analyzer 11 is used to perform particle size analysis on the components of ginger exosomes, monitor the particle size of the substances in the filtrate in real time during the ultrafiltration process, and determine whether the filtrate meets the requirements. If it meets the requirements, a signal is sent and the second switching valve is controlled to connect the third filtration device 10 and the concentrate collection device 12 for collection and storage. If it does not meet the requirements, a signal is sent to control the second switching valve to connect the third filtration device 10 and the transfer tank 8 for circulating filtration. In this embodiment, the particle size analyzer 11 determines that the particle size in the filtrate meets the requirements within the range of 30-150 nm.

[0029] Specifically, it also includes a waste liquid tank 13, and the discharge end of the concentrate collection device 12 is connected to the waste liquid tank 13.

[0030] Specifically, the ginger tissue pretreatment device 1 is used to perform preliminary processing on ginger raw materials, such as crushing and homogenizing. In this embodiment, the ginger tissue pretreatment device 1 adopts a combination of crusher and homogenizer. The ginger tissue pretreatment device 1 is a cell wall breaker, which crushes and homogenizes the ginger raw materials to fully release exosome-related substances from ginger cells, providing a suitable raw material state for subsequent extraction steps.

[0031] Specifically, the gingerol pretreatment device 6 can employ any existing method capable of separating and processing gingerol. In this embodiment, solvent extraction is used: the gingerol pretreatment device 6 is an extraction column, in which ginger raw material containing gingerol is mixed with an organic solvent such as ethanol. Utilizing the difference in solubility of gingerol in the organic solvent and the aqueous phase, gingerol is transferred to the organic phase. Subsequently, the solvent is recovered through separation and distillation to achieve the separation and enrichment of gingerol, while also initially purifying the system, which is beneficial for subsequent exosome extraction.

[0032] Specifically, the pore size of the first filtration device ranges from 5 to 20 μm. In this embodiment, a pore size of 20 μm is specifically selected to remove ginger fiber and large particulate impurities.

[0033] Specifically, the pore size of the second filter device ranges from 0.1 to 5 μm. In this embodiment, a pore size of 0.45 μm is specifically selected to further remove residual particles.

[0034] Specifically, the pore size of the third filtration device ranges from 30 to 150 nm. In this embodiment, a pore size of 0.45 μm is specifically selected to retain target exosomes and remove small molecule impurity proteins.

[0035] Furthermore, pressure sensors and liquid level sensors are installed on the ginger extract storage tank 3 and the transfer tank 8 to monitor the internal pressure and liquid level status, avoid overpressure, abnormal liquid level and other situations, and ensure the safety of ginger sample storage and extraction process. The filling action of the slow-release liquid filling tank 4 is also triggered by the liquid level sensor.

[0036] Furthermore, the input or output ends of the ginger tissue pretreatment device 1, the first filtration device 2, the ginger extract storage tank 3, the gingerol pretreatment device 6, the second filtration device 7, the transfer tank 8, and the third filtration device 10 are equipped with one-way valves to prevent the ginger sample from flowing back during transportation and to ensure that the extraction process proceeds in an orderly manner.

[0037] Specifically, the hybrid pump has multiple power output modes that can be flexibly switched according to the needs of ginger sample delivery, providing power guarantee for stable sample delivery and adapting to the flow and pressure requirements of different extraction stages.

[0038] Work process:

[0039] Ginger tissue pretreatment: Ginger tissue is placed in ginger tissue pretreatment device 1 and subjected to cutting, crushing and other operations to make it into small pieces or pulp suitable for extraction. Then, after preliminary screening by the first filtration device 2, a slow-release solution is added in a certain proportion and injected together into the ginger extract temporary storage tank 3.

[0040] Gingerol pretreatment: The liquid in the ginger extract storage tank 3 is pumped into the gingerol pretreatment device 6 by the first hybrid pump 5. In this unit, gingerol and other components are treated by chemical or physical methods to improve the fluidity, stability and other properties of the extract. The treated liquid is then transported to the microfiltration device 7.

[0041] Secondary filtration: After the liquid enters the second filtration device 7, it is filtered multiple times in the second filtration device 7, and impurity particles are intercepted. The pure ginger extract passes through the filter membrane and enters the transfer tank 8.

[0042] Ultrafiltration and particle size analysis: The transfer tank 8 pumps the third filter device 10 through the second hybrid power pump 9 for the third stage of filtration and screening, and simultaneously delivers the ginger extract after the third stage filtration to the particle size analyzer 11. The particle size analyzer 11 analyzes the particle size distribution of the extract, transmits the analysis results to the relevant display or storage device, and determines whether the particle size meets the requirements. If it does not meet the requirements, the second switching valve is controlled to circulate for the third stage filtration.

[0043] Concentrate collection and waste discharge: After the third stage filtration is completed (meeting the requirements of particle size analysis), the ginger extract with a certain concentration remaining in the transfer tank 8 can be transported to the concentrate collection device 12 for collection; while the waste liquid generated by filtration and the excess liquid generated during the analysis process are discharged into the waste liquid tank 13.

[0044] The foregoing has shown and described the basic principles, main features, and advantages of this utility model. Those skilled in the art should understand that this utility model is not limited to the above embodiments. The embodiments and descriptions in the specification are merely illustrative of the principles of this utility model. Various changes and modifications can be made to this utility model without departing from its spirit and scope, and all such changes and modifications fall within the scope of the claims. The scope of protection of this utility model is defined by the appended claims and their equivalents.

Claims

1. A ginger exosome extraction system, characterized in that: The system includes a ginger tissue pretreatment device (1), a first filtration device (2), a ginger extract storage tank (3), a gingerol pretreatment device (6), a second filtration device (7), a transfer tank (8), and a third filtration device (10). The output end of the ginger tissue pretreatment device (1) is connected to the first filtration device (2), the filtrate end of the first filtration device (2) is connected to the ginger extract storage tank (3), the outlet end of the ginger extract storage tank (3) is connected to the gingerol pretreatment device (6), and the output end of the gingerol pretreatment device (6) is connected to the second filtration device (7). The filtrate end of the second filtration device (7) is connected to the transfer tank (8), and the output end of the transfer tank (8) is connected to the third filtration device. The third filtration device (10) is connected to the filtrate end of the third filtration device (10), which is equipped with a concentrated liquid collection device (12). It also includes a slow-release liquid filling tank (4) and a particle size analyzer (11). The output end of the slow-release liquid filling tank (4) is connected to the ginger extract temporary storage tank (3), and the particle size analyzer (11) is connected to the filtrate end of the third filtration device (10). The filtrate end of the second filtration device (7) is equipped with a first switching valve. One end of the first switching valve is connected to the ginger extract temporary storage tank (3), and the other end is connected to the third filtration device (10). The filtrate end of the third filtration device (10) is equipped with a second switching valve. One end of the second switching valve is connected to the concentrated liquid collection device (12), and the other end is connected to the transfer tank (8).

2. The ginger exosome extraction system according to claim 1, characterized in that: A first hybrid pump (5) is provided between the ginger extract storage tank (3) and the gingerol pretreatment device (6). The inlet end of the first hybrid pump (5) is connected to the ginger extract storage tank (3), and the outlet end of the first hybrid pump (5) is connected to the gingerol pretreatment device (6).

3. The ginger exosome extraction system according to claim 1, characterized in that: A second hybrid power pump (9) is provided between the transfer tank (8) and the third filter device (10). The inlet end of the second hybrid power pump (9) is connected to the transfer tank (8), and the outlet end of the second hybrid power pump (9) is connected to the second filter device (7).

4. The ginger exosome extraction system according to claim 1, characterized in that: It also includes a waste liquid tank (13), the discharge end of the concentrate collection device (12) is connected to the waste liquid tank (13).

5. A ginger exosome extraction system according to any one of claims 1-4, characterized in that: The pore size of the first filter device (2) ranges from 5 to 20 μm.

6. A ginger exosome extraction system according to any one of claims 1-4, characterized in that: The filter pore size of the second filter device (7) ranges from 0.1 to 5 μm.

7. A ginger exosome extraction system according to any one of claims 1-4, characterized in that: The pore size range of the third filtration device (10) is 30–150 nm.

8. A ginger exosome extraction system according to any one of claims 1-4, characterized in that: The input or output end of the ginger tissue pretreatment device (1), the first filtration device (2), the ginger extract storage tank (3), the gingerol pretreatment device (6), the second filtration device (7), the transfer tank (8), and the third filtration device (10) are equipped with one-way valves.

9. A ginger exosome extraction system according to any one of claims 1-4, characterized in that: Pressure sensors and liquid level sensors are provided in the ginger extract temporary storage tank (3) and the transfer tank (8).

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