Gradient dilution sampling device

Abstract

The utility model discloses a gradient dilution sampling device which comprises a liquid taking pipe, a pull-out-tip-shaped liquid taking nozzle arranged at the lower end of the liquid taking pipe, a blocking plate arranged at the upper end of the liquid taking pipe, a piston arranged in the liquid taking pipe, a connecting rod arranged at the top of the piston, a spring arranged between the blocking plate and the pressing handle and vertically and upwards penetrating out of the blocking plate. Scale marks are arranged on the liquid taking pipe and the liquid taking nozzle; a branch pipe is horizontally or obliquely arranged on one side of the liquid taking pipe, one end of the branch pipe is communicated with the liquid taking pipe, the other end of the branch pipe is connected with a U-shaped pipe, a pipe plug is arranged at the other end of the U-shaped pipe, a pressurizing device is inserted into the pipe plug, and a one-way valve flowing from the U-shaped pipe to the liquid taking pipe is arranged on the branch pipe. According to the utility model, multiple groups of samples with different concentrations can be diluted by sampling once, the dilution time is shortened, the inspection error is reduced, the operation is convenient and fast, the working efficiency is high, and the practicability is strong.

Description

Technical Field

[0001] This utility model relates to the field of physiological experimental equipment technology, specifically a gradient dilution sampling device. Background Technology

[0002] In physiological experiments, animal tissues are frequently sectioned, and physiochemical methods are used to visualize the material composition of cell structures. This allows for the analysis and study of cell and tissue metabolism, function, and morphological changes, providing a theoretical basis for physiological cell and tissue structure research. Specifically, the sectioned tissue samples are fixed with a fixative, then dehydrated, embedded in paraffin, sectioned, stained, and finally observed under a microscope. Dehydration is a crucial step, involving sequentially immersing the fixed tissue samples in 75%, 85%, 95%, and anhydrous ethanol for 60 minutes each before paraffin embedding. This process requires preparing different concentrations of ethanol. Traditionally, a graduated cylinder is used to measure a certain amount of anhydrous ethanol, which is then diluted with water to different concentrations, and then placed into separate test tubes. This method allows only one concentration of ethanol to be prepared per sample, resulting in slow operation and low experimental efficiency. Furthermore, differences in sample dilution times can lead to errors in testing. Therefore, there is a need to develop a gradient dilution sampling device that can perform gradient dilution of samples into solutions of different concentrations with only one sample, shortening solution preparation time, reducing testing errors, and is convenient to operate and highly efficient. Utility Model Content

[0003] The purpose of this invention is to provide a gradient dilution sampling device that can perform gradient dilution of samples into solutions of different concentrations with only one sample, shortening solution preparation time, reducing testing errors, and is convenient to operate and highly efficient.

[0004] To achieve the above objectives, this utility model provides the following technical solution: a gradient dilution sampling device, comprising a sampling tube, a sampling nozzle at the lower end of the sampling tube, a blocking plate at the upper end, a piston inside the sampling tube, a connecting rod at the top of the piston, the connecting rod vertically extending upward through the blocking plate and connecting to a pressure handle, a spring between the blocking plate and the pressure handle, and graduation lines on both the sampling tube and the sampling nozzle; a branch tube horizontally or obliquely arranged on one side of the sampling tube, one end of the branch tube being conductively connected to the sampling tube, the other end of the branch tube being connected to a U-shaped tube, a tube plug at the other end of the U-shaped tube, a pressurizing device inserted into the tube plug, and a one-way valve on the branch tube directing the flow from the U-shaped tube to the sampling tube.

[0005] Furthermore, the pressurizing device is either a bulb syringe or a piston syringe.

[0006] Furthermore, a tube cap is fitted onto the lower part of the liquid dispensing nozzle.

[0007] Furthermore, a sealing ring is provided at the connection between the connecting rod and the blocking plate.

[0008] The beneficial effects of this invention are as follows: This invention uses a pressurizing device to add the diluent from the U-shaped tube into the sampling tube through a branch tube and a one-way valve, thus diluting the sample in the sampling tube. After the first concentration of the sample is diluted as required, the lower cap is removed, and the piston is pushed by pressing the handle to add the diluted sample into the first test tube. Then, the cap is replaced, and the pressurizing device is used to continue adding liquid to the sample in the sampling tube for further dilution. The diluted sample is then transferred to the second test tube. This process is repeated to continuously add liquid to dilute the sample and obtain samples of different concentrations to meet experimental requirements. This invention only requires one sampling to dilute multiple groups of samples with different concentrations, shortening the dilution time, reducing testing errors, and is convenient, efficient, and highly practical. Attached Figure Description

[0009] Figure 1 This is a schematic diagram of the overall structure of this utility model;

[0010] In the diagram: 1-liquid nozzle, 2-liquid tube, 3-scale line, 4-piston, 5-blocking plate, 6-connecting rod, 7-pressure handle, 8-spring, 9-pressurizing device, 10-pipe plug, 11-one-way valve, 12-branch pipe, 13-U-shaped tube, 14-pipe cap. Detailed Implementation

[0011] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0012] Please see Figure 1This utility model provides a technical solution: a gradient dilution sampling device, including a liquid collection tube 2, a liquid collection nozzle 1 with a pull-out tip at the lower end of the liquid collection tube 2, a blocking plate 5 at the upper end, a piston 4 inside the liquid collection tube 2, a connecting rod 6 at the top of the piston 4, the connecting rod 6 passing through the blocking plate 5 and connecting to a pressure handle 7, a spring 8 between the blocking plate 5 and the pressure handle 7, and scale lines 3 on both the liquid collection tube 2 and the liquid collection nozzle 1; a branch tube 12 is horizontally or inclinedly arranged on one side of the liquid collection tube 2, one end of the branch tube 12 is connected to the liquid collection tube 2, the other end of the branch tube 12 is connected to a U-shaped tube 13, a tube plug 10 is arranged at the other end of the U-shaped tube 13, a pressurizing device 9 is movably arranged on the tube plug 10, and a one-way valve 11 is arranged on the branch tube 12, the direction of flow from the U-shaped tube 13 to the liquid collection tube 2.

[0013] The pressurizing device 9 is either a bulb syringe or a piston syringe.

[0014] A tube cap 14 is fitted onto the lower part of the dispensing nozzle 1.

[0015] A sealing ring is provided at the connection between the connecting rod 6 and the blocking plate 5.

[0016] Before using this invention, it is necessary to calculate the required concentration of the sample and the volume of diluent to be added. Then, the diluent from the U-shaped tube 13 is added to the sampling tube 2 through the branch tube 12 and the one-way valve 11 via the pressurizing device 9 to dilute the sample in the sampling tube 2. The amount of diluent added is read from the scale on the sampling tube 2. After the first concentration of the sample is diluted as required, the lower cap 14 is removed, and the piston 4 is pushed by pressing the handle 7 to add the diluted sample to the first test tube. Then, the cap 14 is replaced, and the liquid is added to the sample in the sampling tube 2 again via the pressurizing device 9 for further dilution. The diluted sample is then transferred to the second test tube. This process is repeated to continuously add liquid to dilute the sample and obtain samples of different concentrations to meet experimental requirements. This invention only requires one sampling to dilute multiple groups of samples with different concentrations, shortening the dilution time, reducing testing errors, and is convenient, efficient, and highly practical.

Claims

1. A gradient dilution sampling device comprising a liquid taking tube (2), characterized in that: The lower end of the liquid collection tube (2) is provided with a liquid collection nozzle (1) in the shape of a pull-out tip, and the upper end is provided with a block plate (5). A piston (4) is provided inside the liquid collection tube (2). A connecting rod (6) is provided on the top of the piston (4). The connecting rod (6) passes vertically upward through the block plate (5) and connects to the pressure handle (7). A spring (8) is provided between the block plate (5) and the pressure handle (7). Scale lines (3) are provided on both the liquid collection tube (2) and the liquid collection nozzle (1). A branch tube (12) is provided horizontally or inclined on one side of the liquid collection tube (2). One end of the branch tube (12) is connected to the liquid collection tube (2). The other end of the branch tube (12) is connected to the U-shaped tube (13). A tube plug (10) is provided on the other end of the U-shaped tube (13). A pressurizing device (9) is inserted on the tube plug (10). A one-way valve (11) is provided on the branch tube (12) with the direction of flow from the U-shaped tube (13) to the liquid collection tube (2).

2. The gradient dilution sampling device according to claim 1, characterized in that: The pressurizing device (9) is either a bulb syringe or a piston syringe.

3. The gradient dilution sampling device according to claim 1, characterized in that: A tube cap (14) is fitted onto the lower part of the liquid dispensing nozzle (1).

4. The gradient dilution sampling device according to claim 1, characterized in that: A sealing ring is provided at the connection between the connecting rod (6) and the blocking plate (5).

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