Cell culture bag

Abstract

The utility model relates to the technical field of cell biology, in particular to a cell culture bag which comprises a bag body and at least one connector assembly. The connector assembly comprises a liquid discharging pipe and at least two supporting rods supporting the inner wall of the bag body, one end of the liquid discharging pipe is communicated with the bag body, the supporting rods are connected to the end, located in the bag body, of the liquid discharging pipe, and the supporting rods are arranged in the axis direction of the liquid discharging pipe. A liquid discharging gap is formed between every two adjacent supporting rods; the packaging bag has the effect of improving liquid outlet blockage caused by bag body attachment.

Description

Technical Field

[0001] This application relates to the field of cell biology technology, and in particular to a cell culture bag. Background Technology

[0002] Cell culture refers to the technique of separating animal, plant, or microbial cells from the body under sterile conditions and culturing them in a suitable in vitro environment to allow them to grow and multiply. Cell culture is now widely used in various fields such as biology, medicine, and new drug development.

[0003] Currently, cell culture bags have been widely used due to their advantages such as large gas exchange area, which is conducive to cell culture, can obtain more high-quality cells, and can be used for various operations such as direct microscopic observation and monitoring of cell culture process, ensuring sterility throughout the process and convenient operation. For example, the culture bag disclosed in patent CN202519255U includes a bag body and an inlet tube. The four sides of the bag body are heat-sealed to form a sealed edge. Three cylindrical inlet tubes are coaxially and evenly fixed on the upper edge of the bag body. All three inlet tubes can be connected to pipelines. Two symmetrical support rods are coaxially formed at the end of the inlet tube inside the bag.

[0004] Regarding the aforementioned technologies, this type of cell culture bag lacks an outlet for cell culture medium. During the drainage process, the bag body adheres to the surface, causing blockage of the outlet and preventing the complete drainage of the cell culture medium, thus wasting the cultured cell culture medium. Utility Model Content

[0005] To overcome the above problems, this application provides a cell culture bag.

[0006] The cell culture bag provided in this application adopts the following technical solution:

[0007] A cell culture bag includes a bag body and at least one connector assembly, wherein the four sides of the bag body are heat-sealed.

[0008] The connector assembly includes a drain pipe and at least two support rods that support the inner wall of the bag. One end of the drain pipe is connected to the bag body, and the support rods are connected to the drain pipe at one end inside the bag body. The support rods are arranged along the axial direction of the drain pipe, and a drain gap is formed between two adjacent support rods.

[0009] By adopting the above technical solution, the four sides of the bag are thermally sealed, ensuring the airtightness and sterility of the culture environment. The drain pipe in the connector assembly is connected to the bag body to realize the function of cell culture medium inlet and outlet. At least two support rods are connected to one end of the drain pipe located inside the bag body and are set along the axis of the drain pipe. The drain gap formed between adjacent support rods can prevent the bag body from sticking together during the draining process, effectively solving the problem of outlet blockage, thereby ensuring smooth discharge of cell culture medium and improving cell culture efficiency and liquid utilization.

[0010] In one specific implementation, each of the support rods is connected to an arc plate near the drain pipe, and the drain pipe connects the support rods through the arc plates. The openings of the two arc plates face the axis of the drain pipe.

[0011] By adopting the above technical solution, the arc plate makes the connection between the support rod and the drain pipe more stable. At the same time, the design of the two arc plates opening towards the axis of the drain pipe can better distribute the force and enhance the support effect on the inner wall of the bag, thereby effectively preventing the bag from sticking together, ensuring unobstructed drainage gaps, and improving the efficiency of cell fluid drainage.

[0012] In one specific implementation, a fixing rod is integrally provided on the side of the support rod away from the axis of the drain pipe. The fixing rod is connected to the arc plate, and the end of the fixing rod away from the arc plate is shorter than the end of the support rod away from the arc plate.

[0013] By adopting the above technical solution, the setting of the fixing rod can enhance the overall stability of the support rod and prevent the support rod from shifting or deforming during use, thereby more effectively preventing the bag from sticking together and ensuring the smooth flow of the drainage gap; the unique design of the fixing rod makes the end away from the arc plate shorter, which helps to reduce the overall weight while further optimizing the support effect on the inner wall of the bag and improving the smoothness of cell fluid drainage.

[0014] In one specific implementation, a circular plate is connected to the end of the support rod away from the drain pipe, and the circular plate contacts the inner wall of the bag.

[0015] By adopting the above technical solution, the disc can increase the support area of ​​the support rod on the inner wall of the bag, making the bag less prone to sticking during the drainage process, thereby further ensuring the smooth drainage of cell fluid.

[0016] In one specific implementation, the outer wall of the drain pipe is provided with two connecting plates, which are symmetrically arranged along the axis of the drain pipe and are attached to the peripheral wall of the bag.

[0017] By adopting the above technical solution, the connecting plate can enhance the sealing performance between the drain pipe and the bag body, ensure the reliability of the connection between the drain pipe and the bag body, and avoid leakage of cell culture medium.

[0018] In one specific implementation, the connector assembly is provided with two parts, and both drainage pipes are connected to the bag body;

[0019] The cell culture bag also includes an observation assembly located near one of the drainage tubes. The observation assembly includes an observation tube and a Luer connector. The observation tube is connected to the drainage tube at one end outside the bag. The observation tube is used to allow the cell fluid to be drawn for observation. The Luer connector is connected to the end of the observation tube away from the drainage tube.

[0020] By adopting the above technical solution, setting two connector assemblies and connecting two drainage tubes to the bag body, the stability and reliability of the cell culture bag can be improved, while meeting the needs of multi-point drainage. The addition of an observation assembly, especially the combination of the observation tube and the Luer connector, not only enables the periodic extraction and observation of cell fluid but also effectively reduces the risk of external contamination, improving the controllability and safety of the cell culture process. The design of having two connector assemblies enhances structural support and prevents the bag from sticking together.

[0021] In one specific implementation, the observation component further includes a rubber cap that snaps onto the Luer connector.

[0022] By adopting the above technical solution, the Luer connector can be prevented from being exposed to the external environment, effectively reducing the contamination inside the observation tube, ensuring that the cell fluid sample inside the observation tube remains sterile, and improving the accuracy of the observation results.

[0023] In one specific implementation, the system further includes a liquid inlet assembly connected to the drain pipe not connected to the observation tube. The liquid inlet assembly includes an inlet pipe and a clamp. The inlet pipe is used to inject or drain cell fluid, and the inlet pipe and the drain pipe are connected at one end outside the bag. The clamp is connected to the inlet pipe and is used to prevent cell fluid from flowing out of the inlet pipe when cell fluid is not needed.

[0024] By adopting the above technical solution, the addition of the liquid inlet component enables the cell culture bag to have the function of injecting and discharging cell fluid. The liquid inlet pipe is connected to the liquid outlet pipe, realizing the injection of cell suspension, nutrient solution or other reagents and the discharge of cell fluid, which meets various needs in the cell culture process. The design of the clamp can effectively block the cell fluid from flowing out of the liquid inlet pipe when cell fluid is not needed, preventing liquid leakage and contamination, and ensuring the stability and safety of the cell culture environment.

[0025] In one specific implementation, the clamping member includes a clamping plate, the clamping plate having a placement groove for placing the liquid inlet tube, the placement groove being divided into a sliding groove for the liquid inlet tube to slide and a clamping groove for clamping the liquid inlet tube, the sliding groove and the clamping groove being connected.

[0026] By adopting the above technical solution, the placement groove design on the clamp plate allows the inlet pipe to move flexibly within the slide groove, and also enables effective clamping of the inlet pipe through the clamping groove. This reliably prevents cell fluid from flowing out when it is not needed, avoiding contamination and waste. Furthermore, the connection structure between the slide groove and the clamping groove simplifies the operation process and improves ease of use.

[0027] In one specific implementation, the clamp has an opening at the placement groove for removing the inlet pipe, the width of which is slightly smaller than the diameter of the inlet pipe.

[0028] By adopting the above technical solution, it is possible to effectively prevent the clamp from accidentally detaching from the inlet pipe, thereby avoiding the loss of the clamp.

[0029] In summary, this application includes at least one of the following beneficial technical effects:

[0030] 1. The designed cell culture bag has a drain pipe in the connector assembly that is connected to the bag body to realize the function of cell culture medium inlet and outlet. At least two support rods are connected to one end of the drain pipe located inside the bag body and are set along the axis of the drain pipe. The drain gap formed between adjacent support rods can prevent the bag body from sticking together during the draining process, effectively solving the problem of outlet blockage, thereby ensuring smooth discharge of cell culture medium and improving cell culture efficiency and liquid utilization.

[0031] 2. The cell culture bag design features two arc-shaped plates with openings facing the drainage pipe axis, which better distributes the stress and enhances the support for the inner wall of the bag. This effectively prevents the bag from sticking together, ensures unobstructed drainage gaps, and improves the efficiency of cell fluid drainage.

[0032] 3. The designed cell culture bag features a connecting plate that enhances the sealing performance between the drain pipe and the bag body, ensuring the reliability of the connection between the drain pipe and the bag body and preventing leakage of cell culture medium. Attached Figure Description

[0033] Figure 1 This is a schematic diagram of the cell culture bag structure in Example 1.

[0034] Figure 2 This is a schematic diagram of the connector assembly in Example 1.

[0035] Figure 3 This is a schematic diagram of the liquid inlet assembly in Example 1.

[0036] Figure 4 This is a schematic diagram of the structure of the observed component in Example 1.

[0037] Figure 5 This is a schematic diagram of the connector assembly in Example 2.

[0038] Explanation of reference numerals in the attached drawings: 1. Bag body; 11. Positioning hole; 2. Connector assembly; 21. Drain pipe; 211. Connecting plate; 212. Connecting ring; 22. Support rod; 221. Arc plate; 222. Fixing rod; 23. Circular piece; 3. Liquid inlet assembly; 31. Liquid inlet pipe; 311. Closing cap; 312. Flared piece; 32. Clamping piece; 321. Clamping plate; 3211. Placement slot; 3212. Opening; 4. Observation assembly; 41. Observation tube; 42. Luer connector; 43. Rubber cap. Detailed Implementation

[0039] The following is in conjunction with the appendix Figure 1-5 This application will be described in further detail.

[0040] This application discloses a cell culture bag.

[0041] Example 1

[0042] Reference Figure 1 and Figure 2 A cell culture bag includes a bag body 1, at least one connector assembly 2, a liquid inlet assembly 3, and an observation assembly 4. The connector assembly 2 is disposed on the bag body 1, and the liquid inlet assembly 3 and the observation assembly 4 are both disposed on the connector assembly 2.

[0043] Reference Figure 1 In this embodiment, the bag 1 is a rectangular bag with heat-sealed edges around its perimeter. The bag 1 is made of polyethylene material, which has excellent flexibility and chemical inertness, making it very suitable for long-term storage of biological samples such as cell suspensions. In addition to polyethylene, polypropylene or other similar polymer materials can also be selected as alternatives. Two positioning holes 11 are provided on the sealed edge at the top of the bag 1, and the two positioning holes 11 are symmetrically arranged along the center line of the top side of the bag 1 to facilitate the hanging of the bag 1. The four corners of the bag 1 are all rounded to reduce the adhesion of cell fluid.

[0044] Reference Figure 1 and Figure 2In this embodiment, two connector assemblies 2 are provided. The two connector assemblies 2 are distributed at intervals along the length of the side of the bag body 1 away from the positioning hole 11. The connector assembly 2 includes a drain pipe 21 and at least two support rods 22. One end of the drain pipe 21 extends into the bag body 1 and communicates with the bag body 1. Two connecting plates 211 are provided at the connection of the drain pipe 21 to the bag body 1. The two connecting plates 211 are symmetrically arranged along the axis of the drain pipe 21. The connecting plates 211 are integrally formed with the drain pipe 21. The connecting plates 211 are parallel to the axis of the drain pipe 21. The connecting plates 211 are attached to the peripheral wall of the bag body 1 to facilitate the sealing of the drain pipe 21 with the bag body 1. The drain pipe 21 and the connecting plates 211 are both heat-sealed to the bag body 1.

[0045] Reference Figure 1 and Figure 2 In this embodiment, two support rods 22 are preferred. One end of the support rod 22 near the drain pipe 21 is provided with an arc plate 221. The openings 3212 of the two arc plates 221 face the axis of the drain pipe 21. One side of the arc plate 221 is integrally connected to the support rod 22, and the other side is integrally connected to the drain pipe 21. The outer wall of the arc plate 221 is flush with the outer wall of the drain pipe 21. The two arc plates 221 fit against the inner wall of the bag body 1, further supporting the interior of the bag body 1 and facilitating the drainage of cell fluid. The support rod 22 extends from the drain pipe 21 to a position close to the inner surface of the bag body 1. The support rod 22 is a rigid rod integrally manufactured by injection molding, with a slender, straight shape. The support rod 22 is located away from the axis of the drain pipe 21. A fixing rod 222 is integrally connected to one side of the line, and the fixing rod 222 is integrally connected to the arc plate 221. The end of the fixing rod 222 away from the arc plate 221 is shorter than the end of the support rod 22 away from the arc plate 221. The end of the support rod 22 away from the drain pipe 21 is rounded to adapt to the limited installation space and to avoid puncturing the bag body 1 itself. A drain gap is formed between the two support rods 22 for the cell fluid to drain. The two support rods 22 can be set in parallel, or the ends of the two support rods 22 away from the drain pipe 21 can be tilted towards the axis of the drain pipe 21. This prevents the two bag bodies 1 from sticking together when draining the liquid, thereby avoiding blockage of the outlet and facilitating the drain. The support rod 22 is made of PE, which can effectively maintain the shape of the support rod 22 without deformation.

[0046] Reference Figure 1 , Figure 2 and Figure 3The liquid inlet assembly 3 includes an inlet pipe 31 and a clamping member 32. The inlet pipe 31 is located near one of the drain pipes 21. Two connecting rings 212 are coaxially arranged at the outer end of the drain pipe 21 outside the bag body 1, and the two connecting rings 212 are spaced apart along the length of the drain pipe 21. The connecting rings 212 and the drain pipe 21 are integrated. One end of the drain pipe 21 outside the bag body 1 is inserted into the end of the inlet pipe 31 near the bag body 1, and the inlet pipe 31 and the drain pipe 21 are heat-sealed. Before cell culture begins, the cell suspension is injected into the bag body 1 through the inlet pipe 31 and the drain pipe 21. During culture, nutrient solution or other reagents can be added through the inlet pipe 31 to ensure... The inlet tube 31 is cylindrical and preferably made of medical-grade PVC material, which is corrosion-resistant, highly transparent, and facilitates observation of liquid flow. The end of the inlet tube 31 away from the outlet tube 21 is provided with a closing cap 311. The inlet tube 31 and the closing cap 311 are detachably connected by a threaded connection, which can seal the inlet tube 31 and prevent contamination inside the inlet tube 31. Two flared tabs 312 are integrally provided at the end of the inlet tube 31 away from the outlet tube 21. The two flared tabs 312 are symmetrically arranged along the axis of the inlet tube 31, which makes it easy for personnel to hold the inlet tube 31, thereby separating or connecting the closing cap 311 to the inlet tube 31.

[0047] Reference Figure 1 and Figure 3 The clamping member 32 includes a clamping plate 321. The clamping plate 321 has a placement groove 3211 for placing the liquid inlet tube 31. The placement groove 3211 is divided into a sliding groove and a clamping groove. When the liquid inlet tube 31 is in the sliding groove, the clamping plate 321 can be moved by the personnel to slide on the liquid inlet tube 31. When the liquid inlet tube 31 is in the clamping groove, the clamping plate 321 can block the cell fluid and prevent the cell fluid in the bag 1 from flowing out. The clamping plate 321 has an opening 3212 at the placement groove 3211 for taking out the liquid inlet tube 31. The width of the opening 3212 is slightly smaller than the diameter of the liquid inlet tube 31. The placement clamping plate 321 and the liquid inlet tube 31 fall off, resulting in the loss of the clamping plate 321.

[0048] Reference Figure 1 , Figure 2 and Figure 4The observation component 4 is located near the drain pipe 21, which is not connected to the inlet pipe 31. The observation component 4 includes an observation tube 41, a Luer connector 42, and a rubber cap 43. The drain pipe 21, which is not connected to the inlet pipe 31, is located outside the bag body 1 and is inserted into one end of the observation tube 41. The drain pipe 21 and the observation tube 41 are connected by heat sealing. When it is necessary to periodically observe the cell fluid in the bag body 1, the personnel can draw the cell fluid in the bag body 1 through the observation tube 41 for observation. The culture status can be seen. The specific cycle depends on the personnel requirements. The Luer connector 42 is located at the end of the observation tube 41 away from the drain pipe 21 and is inserted into the observation tube 41. The Luer connector 42 and the observation tube 41 are press-fitted. The Luer connector 42 seals the inside of the observation tube 41, reducing the possibility of contamination inside the observation tube 41. The rubber cap 43 is fastened to the Luer connector 42 to prevent contamination of the Luer connector 42.

[0049] The implementation principle of Example 1 is as follows: When a cell culture bag is needed, check whether the bag body 1, connector assembly 2, liquid inlet assembly 3, and observation assembly 4 are intact. First, inject the cell suspension into the bag body 1 through the liquid inlet tube 31. Then, clamp the liquid inlet tube 31 with the clamp 321. According to experimental needs, add an appropriate amount of nutrient solution or other reagents. After a certain period of culture, remove the rubber cap 43. Personnel can then draw cell fluid through the observation tube 41 for observation. After the drawing is completed, fasten the rubber cap 43 to the observation tube 41. Following the above steps, the cells can be observed. After periodic observation, when cell solution is needed, first confirm that bag 1 is not stuck. Then, remove the clamp 321 and closing cap 311 from the inlet tube 31. The cell solution can be drawn from bag 1 through the inlet tube 31. If it is necessary to temporarily stop the drainage, the inlet tube 31 can be clamped with the clamp 321 to prevent backflow of outside air. After the extraction is completed, the inlet tube 31 should be clamped with the clamp 321 in time. After the cell solution is drained, bag 1 should be cleaned and disinfected for the next use. The support rod 22 plays a supporting role to prevent bag 1 from sticking and ensure smooth drainage of liquid.

[0050] Example 2

[0051] Reference Figure 4 and Figure 5 The difference between this embodiment and embodiment 1 is that the connector assembly 2 also includes two circular pieces 23, which correspond one-to-one with the support rod 22. The circular pieces 23 are integrally set at the end of the support rod 22 away from the drain pipe 21. The circular pieces 23 are attached to the inner wall of the bag body 1. The circular pieces 23 have a larger supporting area for the bag body 1, making it easier to support the bag body 1 and facilitate the flow of cell fluid through the drain pipe 21 to the inlet pipe 31, thereby allowing the cell fluid to flow out.

[0052] The implementation principle of Example 2 is as follows: When cell fluid is needed, the personnel remove the clamp 321 and the closing cap 311 on the inlet tube 31, the disc 23 supports the inside of the bag 1, and the personnel can extract the cell fluid in the bag 1 through the inlet tube 31.

[0053] The above are all preferred embodiments of this application, and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A cell culture bag, characterized in that: It includes a bag body (1) and at least one connector assembly (2), wherein the four sides of the bag body (1) are heat-sealed. The connector assembly (2) includes a drain pipe (21) and at least two support rods (22) that support the inner wall of the bag body (1). One end of the drain pipe (21) is connected to the bag body (1), and the support rod (22) is connected to the end of the drain pipe (21) located inside the bag body (1). The support rod (22) is arranged along the axial direction of the drain pipe (21), and a drain gap is formed between two adjacent support rods (22).

2. The cell culture bag according to claim 1, characterized in that: Each of the support rods (22) is connected to an arc plate (221) near the drain pipe (21). The drain pipe (21) connects the support rods (22) through the arc plates (221). The openings (3212) of the two arc plates (221) face the axis of the drain pipe (21).

3. A cell culture bag according to claim 2, characterized in that: A fixing rod (222) is integrally provided on the side of the support rod (22) away from the axis of the drain pipe (21). The fixing rod (222) is connected to the arc plate (221). The end of the fixing rod (222) away from the arc plate (221) is shorter than the end of the support rod (22) away from the arc plate (221).

4. A cell culture bag according to claim 1, characterized in that: The support rod (22) is connected to a disc (23) at the end away from the drain pipe (21), and the disc (23) is in contact with the inner wall of the bag body (1).

5. A cell culture bag according to any one of claims 1-4, characterized in that: The outer wall of the drain pipe (21) is provided with two connecting plates (211). The two connecting plates (211) are symmetrically arranged along the axis of the drain pipe (21). The connecting plates (211) are attached to the periphery of the bag body (1).

6. A cell culture bag according to claim 5, characterized in that: The connector assembly (2) is provided in two parts, and both of the drain pipes (21) are connected to the bag body (1); The cell culture bag also includes an observation assembly (4), which is located near one of the drainage tubes (21). The observation assembly (4) includes an observation tube (41) and a Luer connector (42). The observation tube (41) is connected to the drainage tube (21) at one end outside the bag body (1). The observation tube (41) is used to allow the cell fluid to be drawn for observation to pass through. The Luer connector (42) is connected to the end of the observation tube (41) away from the drainage tube (21).

7. A cell culture bag according to claim 6, characterized in that: The observation assembly (4) also includes a rubber cap (43) that engages with the Luer connector (42).

8. A cell culture bag according to claim 6, characterized in that: It also includes a liquid inlet assembly (3), which is connected to the drain pipe (21) that is not connected to the observation tube (41). The liquid inlet assembly (3) includes a liquid inlet pipe (31) and a clamp (32). The liquid inlet pipe (31) is used to inject or drain cell fluid. The liquid inlet pipe (31) and the drain pipe (21) are connected at one end outside the bag body (1). The clamp (32) is connected to the liquid inlet pipe (31). When cell fluid is not needed, the clamp (32) is used to prevent cell fluid from flowing out of the liquid inlet pipe (31).

9. A cell culture bag according to claim 8, characterized in that: The clamping member (32) includes a clamping plate (321), which has a placement groove (3211) for placing the liquid inlet pipe (31). The placement groove (3211) is divided into a sliding groove for sliding the liquid inlet pipe (31) and a clamping groove for clamping the liquid inlet pipe (31). The sliding groove and the clamping groove are connected.

10. A cell culture bag according to claim 9, characterized in that: The clamp (321) has an opening (3212) at the placement groove (3211) for the liquid inlet pipe (31) to be taken out. The width of the opening (3212) is slightly smaller than the diameter of the liquid inlet pipe (31).

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