NK cell culture device

Abstract

The utility model discloses an NK (Natural Killer) cell culture device, which relates to the technical field of biological cell culture and comprises a base, a support structure, a power transmission system, a bearing and adjusting mechanism and a motion conversion component, the base is used for bearing and stabilizing other parts, a first support seat is fixedly arranged on the base and is used for supporting a motor and a transmission part thereof, and a second support seat is fixedly arranged on the base and is used for supporting the motor. The motor drives the first gear to rotate through the output shaft, the first gear is meshed with the first rotating shaft, one end of the first rotating shaft is connected with the second gear, the second gear is connected with the rotating plate through a key groove, the rotating plate is connected with the sliding block through the second rotating shaft, the sliding block slides along the rack, and the rack is fixed to the fixed guide rail. The NK cell culture device provided by the utility model effectively solves the problem of cell sedimentation caused by the gravity action, promotes the uniform distribution of cells, improves the culture efficiency, reduces the operation frequency of experimenters, reduces the pollution risk, and meets diversified experiment requirements.

Description

Technical Field

[0001] This utility model relates to the field of biological cell culture technology, specifically to an NK cell culture device. Background Technology

[0002] In the fields of biomedical research and clinical applications, NK cell (natural killer cell) culture technology is of great significance. As an important immune cell, NK cells play a crucial role in anti-tumor, antiviral, and immune regulation. However, existing NK cell culture technologies still have many shortcomings, especially in the design and use of cell culture devices. Traditional methods typically involve laying cell culture bags flat in an incubator. Due to gravity, NK cells tend to settle to the bottom of the bag after aggregation, resulting in uneven cell distribution and affecting their growth efficiency and activity. Furthermore, to address cell sedimentation, researchers need to frequently open the incubator and tap the culture bags to redisperse the cells. This repeated manual intervention not only increases the complexity of the operation but also introduces potential contamination risks, seriously affecting the stability and safety of the culture process. Therefore, developing an NK cell culture device that can effectively prevent cell sedimentation, reduce manual intervention, and improve culture efficiency has become an urgent technical challenge. This invention aims to provide an innovative solution to overcome the shortcomings of existing technologies. Utility Model Content

[0003] This invention addresses the technical problems in existing NK cell culture processes, such as cells settling at the bottom of the culture bag due to gravity, affecting cell growth, and the risk of contamination due to frequent handling by laboratory personnel. It proposes an NK cell culture device that achieves dynamic adjustment through a mechanical transmission mechanism. This device aims to solve the aforementioned technical problems while improving cell culture efficiency and safety.

[0004] This invention provides an NK cell culture device, including a base, a support structure, a power transmission system, a support and adjustment mechanism, and a motion conversion component. The base serves as the fundamental support for the entire device, supporting and stabilizing other components. The base is made of a rigid material and features multiple mounting holes to accommodate fixation requirements in different experimental environments. Furthermore, the geometry of the base is optimized to ensure balance when supporting the components, thereby improving the overall reliability of the device.

[0005] Furthermore, the first support base is fixedly installed on the base to support the motor and its related transmission components; the frame connects the base and the first support base, forming an integral frame structure. The frame is made of high-strength alloy material and has multiple reinforcing ribs inside to enhance its resistance to deformation. In particular, the frame is also designed with multiple reserved holes for installing subsequent components, facilitating assembly and maintenance.

[0006] Furthermore, the motor drives the first gear to rotate via its output shaft. The first gear meshes with the first rotating shaft, and one end of the first rotating shaft is connected to the second gear. Both the first and second gears are standard involute gears to ensure smoothness and precision during transmission. The first rotating shaft is fixed within the first support base by bearings, which are double-row deep groove ball bearings to withstand radial and axial loads. Furthermore, the second gear is connected to the rotating plate via a keyway to achieve synchronous rotation.

[0007] Specifically, the rotating plate is connected to the slider via a second rotating shaft, and the slider slides along the rack. The rack is fixed to a fixed guide rail, which is bolted to the side of the frame. The bottom surface of the slider is equipped with a ball bearing structure to reduce friction and improve sliding efficiency. Furthermore, the top of the slider is connected to the rotating plate via a pin, and both ends of the pin are equipped with anti-detachment caps to prevent loosening or falling off during operation.

[0008] Furthermore, the fixed guide rail guides the slider to move along a predetermined trajectory. The slide frame is mounted on the fixed guide rail and supports the second support base and other components. Limiting protrusions are provided on both sides of the slide frame, which engage with the grooves of the fixed guide rail to limit the range of motion of the slide frame and prevent it from exceeding the preset trajectory. In particular, the bottom surface of the slide frame is provided with a lubricating coating to reduce the coefficient of friction and extend its service life.

[0009] Furthermore, the third gear meshes with the lead screw, converting the rotational motion into linear motion, thereby driving the sliding base to move horizontally. The surface of the lead screw is hardened to improve wear resistance. The sliding base uses guide grooves to restrict the movement trajectory; the width of the guide grooves fits tightly with the protruding portion of the sliding base to ensure smooth movement. Specifically, the upper surface of the sliding base is provided with anti-slip pads to secure the NK cell culture bag and prevent displacement during movement.

[0010] Furthermore, the support tube is used to support the NK cell culture bag and prevent it from tilting or falling. The inner wall of the support tube is lined with an elastic pad to protect the culture bag from damage. An adjusting groove cooperates with an adjusting slide block, allowing the position of the support tube to be flexibly adjusted according to the height of the culture bag. In particular, a locking screw is provided at the end of the adjusting slide block to fix the position of the support tube and prevent loosening during operation.

[0011] Furthermore, a return spring is installed at the rear end of the sliding base to ensure that the sliding base automatically returns to its initial position without external force. An adjusting clamp is installed at the front end of the sliding base to precisely adjust its position to accommodate culture bags of different sizes. Specifically, the clamping surface of the adjusting clamp is equipped with a rubber pad to increase friction while protecting the surface of the culture bag from damage.

[0012] In the above technical solution, this utility model achieves dynamic adjustment of the NK cell culture bag through a mechanical transmission mechanism. Specifically, S1: After the motor starts, the output shaft drives the first gear to rotate, and the first gear drives the first rotating shaft and the second gear to rotate synchronously; S2: The second gear drives the rotating plate to rotate, and the rotating plate drives the slider to slide along the rack through the second rotating shaft; S3: The linear motion of the slider is transmitted to the sliding frame through the fixed guide rail, and the sliding frame drives the second support base and subsequent components to move in coordination; S4: The third gear meshes with the lead screw, converting the rotational motion into linear motion, driving the sliding base to move horizontally; S5: The movement of the sliding base is limited by the guide groove, and at the same time, the support cylinder moves with the sliding base, realizing dynamic adjustment of the culture bag.

[0013] Furthermore, the technical effects of this invention are as follows: The mechanical transmission mechanism design solves the problem of NK cells settling to the bottom of the culture bag due to gravity, promoting uniform cell distribution and significantly improving cell culture efficiency. In particular, the automated design reduces the frequency of manual operation by experimental personnel, lowers the potential risk of contamination from frequent opening of the incubator, and improves the safety and reliability of the experiment. In addition, the device is equipped with a height adjustment mechanism and adjustment clamps, which can adapt to culture bags of different sizes and shapes to meet diverse experimental needs. The overall structure is compact, with tight cooperation between components, ensuring stable and reliable operation, and is easy to maintain and operate.

[0014] In summary, this invention effectively addresses the shortcomings of existing technologies through innovative mechanical transmission and automated design, providing an efficient and safe solution for NK cell culture. Attached Figure Description

[0015] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in this utility model. For those skilled in the art, other drawings can be obtained based on these drawings.

[0016] Figure 1 A schematic diagram of the overall structure provided for an embodiment of this utility model;

[0017] Figure 2 Provided for the embodiments of this utility model Figure 1Schematic diagram of the structure at point A;

[0018] Figure 3 Provided for the embodiments of this utility model Figure 1 Schematic diagram of the structure at point B;

[0019] Figure 4 Provided for the embodiments of this utility model Figure 1 A schematic diagram of the back structure;

[0020] Figure 5 Provided for the embodiments of this utility model Figure 4 Schematic diagram of the structure at point C;

[0021] Figure 6 Provided for the embodiments of this utility model Figure 4 A partial structural diagram.

[0022] Explanation of reference numerals in the attached figures:

[0023] 1. Base; 2. First support seat; 3. Frame; 4. Motor; 5. Output shaft; 6. First gear; 7. First fixed seat; 8. First rotating shaft; 9. Second gear; 10. Rotating plate; 11. Second rotating shaft; 12. Slider; 13. Rack; 14. Fixed guide rail; 15. Slide frame; 16. Second support seat; 17. Second fixed seat; 18. Third rotating shaft; 19. Third gear; 20. Mounting seat; 21. Lead screw; 22. Drive seat; 23. Sliding base; 24. Guide groove; 25. Adjusting clamp; 26. Return spring; 27. Adjusting groove; 28. Support cylinder; 29. ​​Adjusting slide block. Detailed Implementation

[0024] To enable those skilled in the art to better understand the technical solution of this utility model, the present utility model will be further described in detail below with reference to the accompanying drawings.

[0025] This invention provides an NK cell culture device, combined with attached... Figure 1 To be continued Figure 6 The specific structure and component numbers marked herein provide a detailed description of the specific embodiments of this utility model. The device is designed to solve the problem in the prior art where NK cells settle to the bottom of the culture bag due to gravity. Simultaneously, its automated design reduces the frequency of manual operation by laboratory personnel, lowers the risk of contamination, and improves cell culture efficiency and safety.

[0026] like Figure 1As shown, the core components of the entire device include a base 1, a first support base 2, a frame 3, a motor 4, an output shaft 5, a first gear 6, a first rotating shaft 8, a second gear 9, a rotating plate 10, a slider 12, a rack 13, a fixed guide rail 14, a sliding frame 15, a lead screw 21, and a support cylinder 28. These components achieve dynamic adjustment through a mechanical transmission mechanism to improve the uniformity of NK cell distribution during culture. In specific implementation, the base 1, as the basic support of the entire device, is made of high-strength stainless steel with a rust-proof surface treatment to ensure it will not be damaged by environmental factors during long-term use. The base 1 is designed with multiple mounting holes, allowing it to be securely installed on a laboratory workbench or inside an incubator using bolts or other fasteners, ensuring the stability of the device during operation. In addition, the geometry of the base 1 has been optimized, and its bottom surface is equipped with an anti-slip pad to further enhance the overall balance and vibration resistance of the device.

[0027] The first support base 2 is fixedly mounted on the base 1 to support the motor 4 and its related transmission components. The first support base 2 is made of aluminum alloy, which is lightweight and high-strength. It contains a bearing housing to fix the first rotating shaft 8 and provide stable support for the output shaft 5 of the motor 4. The frame 3 connects the base 1 and the first support base 2, forming an integral frame structure. The frame 3 is made of high-strength alloy material and has multiple reinforcing ribs inside to enhance its resistance to deformation. Specifically, the side of the frame 3 has multiple pre-drilled holes for installing subsequent components, facilitating assembly and maintenance. For example, the fixed guide rail 14 is bolted to the side of the frame 3. The surface of the fixed guide rail 14 is hardened to improve wear resistance and extend its service life.

[0028] Motor 4 is the power source for the entire device, and its output shaft 5 is directly connected to the first gear 6. When motor 4 starts, output shaft 5 drives the first gear 6 to rotate, and the first gear 6 meshes with the first rotating shaft 8, thereby transmitting power to the first rotating shaft 8. One end of the first rotating shaft 8 is connected to the second gear 9 through a keyway. The first rotating shaft 8 is fixed in the first support seat 2 by a double-row deep groove ball bearing. This bearing design can withstand radial and axial loads, ensuring smoothness during transmission. The second gear 9 is connected to the rotating plate 10 through a keyway to achieve synchronous rotation. The rotating plate 10 is connected to the slider 12 through the second rotating shaft 11, and the slider 12 slides along the rack 13. The rack 13 is fixed on the fixed guide rail 14, and its surface is precision machined to ensure smooth sliding of the slider 12. The bottom surface of the slider 12 is provided with a ball bearing structure. This design significantly reduces the friction between the slider 12 and the rack 13, improving sliding efficiency. In addition, the top of the slider 12 is connected to the rotating plate 10 by a pin, and anti-detachment caps are provided at both ends of the pin to prevent loosening or falling off during operation.

[0029] The slide frame 15 is mounted on the fixed guide rail 14 and supports the second support base 16 and other components. Limiting protrusions are provided on both sides of the slide frame 15, which engage with the grooves of the fixed guide rail 14 to limit the movement range of the slide frame 15 and prevent it from exceeding the preset trajectory. The bottom surface of the slide frame 15 is coated with a lubricating coating made of polytetrafluoroethylene (PTFE), which has excellent low-friction characteristics and can effectively reduce the coefficient of friction between the slide frame 15 and the fixed guide rail 14, thereby extending its service life. The third gear 19 meshes with the lead screw 21, converting the rotational motion into linear motion, thereby driving the sliding base 23 to move horizontally. The surface of the lead screw 21 is hardened to improve wear resistance. The sliding base 23 restricts its movement trajectory through a guide groove 24, the width of which closely matches the protrusions of the sliding base 23 to ensure smooth movement. The upper surface of the sliding base 23 is provided with an anti-slip pad. This anti-slip pad is made of silicone material, which has good flexibility and friction properties, and can firmly fix the NK cell culture bag to prevent it from shifting during movement.

[0030] The support cylinder 28 is used to support the NK cell culture bag and prevent it from tilting or falling. The inner wall of the support cylinder 28 is lined with an elastic pad made of medical-grade silicone material, which has excellent elasticity and cushioning properties to protect the culture bag from damage. The adjusting slide 27 cooperates with the adjusting slide 29 to flexibly adjust the position of the support cylinder 28 according to the height of the culture bag. A locking screw is provided at the end of the adjusting slide 29 to fix the position of the support cylinder 28 and prevent loosening during operation. A return spring 26 is installed at the rear end of the sliding base 23 to ensure that the sliding base 23 automatically returns to its initial position without external force. An adjusting clamp 25 is installed at the front end of the sliding base 23 to precisely adjust its position to accommodate culture bags of different sizes. The clamping surface of the adjusting clamp 25 is provided with a rubber pad, which has a high coefficient of friction and good flexibility, increasing friction while protecting the surface of the culture bag from damage.

[0031] The operating principle of the entire device is as follows: S1 After the motor 4 starts, the output shaft 5 drives the first gear 6 to rotate. The first gear 6 drives the first rotating shaft 8 to rotate through meshing. One end of the first rotating shaft 8 is connected to the second gear 9 through a keyway, thereby transmitting power to the second gear 9. S2 The second gear 9 drives the rotating plate 10 to rotate. The rotating plate 10 drives the slider 12 to slide along the rack 13 through the second rotating shaft 11. S3 The linear motion of the slider 12 is transmitted to the sliding frame 15 through the fixed guide rail 14. The sliding frame 15 drives the second support seat 16 and subsequent components to move in coordination. S4 The third gear 19 meshes with the lead screw 21, converting the rotational motion into linear motion, driving the sliding base 23 to move horizontally. S5 The movement of the sliding base 23 is limited by the guide groove 24. At the same time, the support cylinder 28 moves with the sliding base 23, realizing the dynamic adjustment of the culture bag. Through the above steps, the device can realize the periodic swaying or vibration of the NK cell culture bag, so that the cells are evenly distributed in the culture medium and avoid sedimentation caused by gravity.

[0032] In practical applications, this device is widely applicable to NK cell culture scenarios in various biological laboratories. For example, in the field of tumor immunology research, researchers need to culture large quantities of NK cells for cytotoxic activity testing. In traditional methods, cells tend to settle to the bottom of the culture bag, resulting in unsatisfactory culture results. This device solves this problem through its mechanical transmission mechanism design, significantly improving cell culture efficiency. Furthermore, the automated design reduces the frequency of manual operation by laboratory personnel, lowers the potential risk of contamination from frequent opening of the incubator, and improves the safety and reliability of the experiment.

[0033] In summary, this invention effectively addresses the shortcomings of existing technologies through innovative mechanical transmission and automated design, providing an efficient and safe solution for NK cell culture.

[0034] The foregoing description only illustrates certain exemplary embodiments of the present invention. Undoubtedly, those skilled in the art can modify the described embodiments in various ways without departing from the spirit and scope of the present invention. Therefore, the above drawings and descriptions are illustrative in nature and should not be construed as limiting the scope of protection of the claims of the present invention.

Claims

1. An NK cell culture device, characterized in that, The system includes a base (1), a first support base (2), a frame (3), a motor (4), an output shaft (5), a first gear (6), a first rotating shaft (8), a second gear (9), a rotating plate (10), a slider (12), a rack (13), a fixed guide rail (14), a slide frame (15), a lead screw (21), a sliding base (23), a support cylinder (28), and an adjusting groove (27). The base (1) is used to support and stabilize other components. The first support base (2) is fixedly installed on the base (1) and is used to support the motor (4) and its transmission components. The motor (4) drives the first gear (6) to rotate through the output shaft (5). The first gear (6) meshes with the first rotating shaft (8). One end of 8) is connected to the second gear (9), the second gear (9) is connected to the rotating plate (10) through the keyway, the rotating plate (10) is connected to the slider (12) through the second rotating shaft (11), the slider (12) slides along the rack (13), the rack (13) is fixed on the fixed guide rail (14), the slide frame (15) is installed on the fixed guide rail (14) and carries the second support seat (16) and other components, the third gear (19) meshes with the lead screw (21) to convert the rotational motion into linear motion, thereby driving the sliding base (23) to move horizontally, the support cylinder (28) is used to support the NK cell culture bag, the adjusting groove (27) cooperates with the adjusting slide (29) to adjust the position of the support cylinder (28).

2. The NK cell culture device according to claim 1, characterized in that, The base (1) is made of rigid material and is designed with multiple mounting holes. The geometry of the base (1) is optimized to ensure balance when supporting the components.

3. The NK cell culture device according to claim 2, characterized in that, The bottom surface of the base (1) is provided with an anti-slip pad layer to further enhance the overall balance and vibration resistance.

4. The NK cell culture device according to claim 1, characterized in that, The frame (3) is made of high-strength alloy material and has multiple reinforcing ribs inside to enhance its resistance to deformation. The frame (3) is also designed with multiple reserved holes for installing subsequent components.

5. The NK cell culture device according to claim 1, characterized in that, The bottom surface of the slider (12) is provided with a ball bearing structure to reduce friction. The top of the slider (12) is connected to the rotating plate (10) by a pin. Anti-detachment caps are provided at both ends of the pin to prevent loosening or falling off during operation.

6. The NK cell culture device according to claim 1, characterized in that, The sliding frame (15) is provided with limiting protrusions on both sides. The limiting protrusions cooperate with the groove of the fixed guide rail (14) to limit the movement range of the sliding frame (15). The bottom surface of the sliding frame (15) is provided with a lubricating coating to reduce the coefficient of friction.

7. The NK cell culture device according to claim 1, characterized in that, The surface of the lead screw (21) is hardened to improve wear resistance. The sliding base (23) restricts the movement trajectory through the guide groove (24). The width of the guide groove (24) is closely matched with the protrusion of the sliding base (23).

8. The NK cell culture device according to claim 1, characterized in that, The inner wall of the support tube (28) is provided with an elastic pad to protect the culture bag from damage.

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