Full-automatic cell culture medium fermentation tank with uniform sterilization function

Abstract

The utility model relates to the technical field of cell culture, in particular to a cell culture medium full-automatic fermentation tank uniform in sterilization, which comprises a tank body, a sight glass, a PH tester, an upper cover and a scraping device, the scraping device comprises a movable circular ring, a scraping plate, a pushing assembly, an auxiliary assembly and a temperature control assembly, and the movable circular ring is in sliding connection with the tank body; the scraping plate is fixedly connected with the movable circular ring, the pushing assembly is arranged on the movable circular ring, the auxiliary assembly is arranged on the tank body, and the temperature control assembly is arranged on the tank body. In the discharging process after cell culture, the movable circular ring and the scraping plate are arranged in an inner cavity of the tank body, and the movable circular ring is driven by the pushing assembly to move in the inner cavity of the tank body; the movable circular ring drives the scraping plate to scrape raw materials on the inner surface of the tank body, so that cells are prevented from being adhered to the inner wall of the tank body, and the productivity of cell culture is improved.

Description

Technical Field

[0001] This utility model relates to the field of cell culture technology, and in particular to a fully automated fermenter for sterilized and uniformly fermented cell culture medium. Background Technology

[0002] During the discharge process after fermentation, existing cell culture fermenters are prone to sedimentation at the bottom of the tank. Excessive sedimentation can easily cause blockages during discharge, and the discharge speed is relatively slow. Furthermore, it is not convenient to clean the tank in the later stages, as the bottom of the tank cannot be cleaned effectively.

[0003] Prior art CN209669246U discloses a cell culture fermenter, including a tank body and a top cover, which are interlocked. A stirring paddle is disposed through the upper part of the top cover. Stirring shafts are disposed on both sides of the bottom of the tank body, and small motors are disposed on both sides of the bottom of the tank body. The small motors are movably connected to the stirring shafts. A groove is formed on the front surface of the stirring paddle, and a fixing member is disposed at the front end of the groove. Several sets of mounting plates are fixedly installed on both sides of the stirring paddle. Stirring rods are fixedly installed on the upper and lower surfaces of the mounting plates. A support rod is disposed inside the stirring paddle. Furthermore, the front surface of the support rod is provided with several sets of positioning holes at equal intervals, and a brush is provided at the lower end of the support rod. A sight glass and a pH tester are provided on the front surface of the tank, with the pH tester located below the sight glass. A discharge port is provided at the center of the lower surface of the tank. By setting the support rod and the brush inside the agitator, the rotation of the agitator drives the support rod to rotate, which in turn drives the brush to rotate, thereby enabling the bottom of the tank to be brushed. This eliminates the trouble of manual brushing. After rinsing with water, the contents are discharged from the discharge port, thus completing the cleaning of the bottom of the tank and making the tank cleaning more convenient.

[0004] However, when using the above method, a lot of raw material will adhere to the inner surface of the tank during the discharge process after cell culture, resulting in incomplete discharge and thus reducing the productivity of cell culture. Utility Model Content

[0005] The purpose of this invention is to provide a fully automated fermenter for sterilized and uniform cell culture medium, which enables the scraping of raw materials from the inner surface of the tank during the discharge process after cell culture, thereby improving the productivity of cell culture.

[0006] To achieve the above objectives, this utility model provides a fully automated fermenter for sterilized and uniform cell culture medium, comprising a tank body, a sight glass, a pH meter, and a top cover. The sight glass and the pH meter are provided on the front surface of the tank body, with the pH meter located below the sight glass. The top cover is detachably connected to the tank body and is located on one side of the tank body. It also includes a scraping device.

[0007] The scraping device includes a movable ring, a scraper, a pushing component, an auxiliary component, and a temperature control component. The movable ring is slidably connected to the tank and located on one side of the tank. The scraper is fixedly connected to the movable ring and located on one side of the movable ring. The pushing component is disposed on the movable ring. The auxiliary component is disposed on the tank. The temperature control component is disposed on the tank.

[0008] The pushing component includes a positioning cylinder and a push rod. The positioning cylinder is fixedly connected to the upper cover and located on one side of the upper cover. The push rod is fixedly connected to the moving ring and slidably connected to the positioning cylinder.

[0009] The pushing component further includes a force-applying rod, which is fixedly connected to the push rod and located on one side of the push rod.

[0010] The auxiliary component includes a conical barrel and a limiting ring. The conical barrel is fixedly connected to the tank body and is located at the bottom of the inner cavity of the tank body; the limiting ring is fixedly connected to the tank body and is located on one side of the tank body.

[0011] The temperature control component includes a heating element and a temperature display. The heating element is disposed in a mounting groove formed by the outer wall of the tank and the inner cavity; the temperature display is disposed on the tank.

[0012] This invention relates to a fully automated fermenter for sterilizing and homogenizing cell culture medium, comprising a tank body, a sight glass, a pH meter, a top cover, and a scraping device. The scraping device includes a moving ring, a scraper, a pushing component, an auxiliary component, and a temperature control component. During the discharge process after cell culture, the moving ring and the scraper are arranged in the inner cavity of the tank. The pushing component drives the moving ring to move within the inner cavity of the tank body, causing the moving ring to drive the scraper to scrape off the raw material on the inner surface of the tank body, preventing cells from adhering to the inner wall of the tank body, thereby improving the productivity of cell culture. Attached Figure Description

[0013] To more clearly illustrate the technical solutions in the embodiments of this application or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below.

[0014] Figure 1 This is a schematic diagram of the overall structure of the fully automated fermenter for sterilized and uniformly fermented cell culture medium according to the first embodiment of this utility model.

[0015] Figure 2 This is a schematic diagram of the scraping device according to the first embodiment of the present invention.

[0016] Figure 3This is a front sectional view of the tank body according to the first embodiment of this utility model.

[0017] Figure 4 This is a schematic diagram of the structure of the temperature control component for uniform sterilization according to the first embodiment of this utility model.

[0018] In the diagram: 101-Tank body, 102-Sight glass, 103-PH tester, 104-Top cover, 105-Moving ring, 106-Scraper, 107-Positioning cylinder, 108-Push rod, 109-Force rod, 110-Conical barrel, 111-Limiting ring, 112-Heating element, 113-Temperature display. Detailed Implementation

[0019] The embodiments of the present invention are described in detail below. Examples of the embodiments are shown in the accompanying drawings. The embodiments described below with reference to the accompanying drawings are exemplary and intended to explain the present invention, but should not be construed as limiting the present invention.

[0020] The first embodiment of this application is as follows:

[0021] Please see Figures 1 to 4 ,in Figure 1 This is a schematic diagram of the overall structure of a fully automated fermenter for sterilized cell culture medium. Figure 2 This is a schematic diagram of the scraping device. Figure 3 This is a front sectional view of the tank. Figure 4 This is a schematic diagram of the temperature control component. This utility model provides a fully automated fermenter for uniformly sterilized cell culture medium: it includes a tank body 101, a sight glass 102, a pH meter 103, a top cover 104, and a scraping device. The scraping device includes a moving ring 105, a scraper 106, a pushing component, an auxiliary component, and a temperature control component. The pushing component includes a positioning cylinder 107, a push rod 108, and a force-applying rod 109. The auxiliary component includes a conical barrel 110 and a limiting ring 111. The temperature control component includes a heating element 112 and a temperature display 113. The aforementioned solution solves the problem that during the discharge process after cell culture, a large amount of raw material adheres to the inner surface of the tank body 101, resulting in incomplete discharge and reduced cell culture productivity. It is understood that the aforementioned solution can be used to scrape off the raw material from the inner surface of the tank body 101 during the discharge process after cell culture, thereby improving cell culture productivity. It can also be used to facilitate temperature adjustment inside the tank body 101.

[0022] In this specific embodiment, the front surface of the tank 101 is provided with the endoscope 102 and the pH tester 103. The pH tester 103 is located below the endoscope 102. The upper cover 104 is detachably connected to the tank 101 and is located on one side of the tank 101. The tank 101 is used for cell culture and fermentation. The upper cover 104 is engaged with the tank 101. The endoscope 102 and the pH tester 103 are both located on the front side of the tank 101. The endoscope 102 is used to observe the internal condition of the tank 101, and the pH tester 103 is used to test the pH value change of the cells inside the tank 101 during fermentation. All of the above are existing technologies.

[0023] The movable ring 105 is slidably connected to the tank body 101 and located on one side of the tank body 101. The scraper 106 is fixedly connected to the movable ring 105 and located on one side of the movable ring 105. The pushing component is disposed on the movable ring 105. The auxiliary component is disposed on the tank body 101. The temperature control component is disposed on the tank body 101. The movable ring 105 is located in the inner cavity of the tank body 101. The scraper 106 is disposed at the bottom of the movable ring 105, and the outer surface of the scraper 106 is in close contact with the inner surface of the tank body 101. The pushing component is disposed on one side of the upper surface of the movable ring 105 and penetrates the upper cover 104. The operator can move the movable ring 105 through the pushing component, so that the movable ring 105... 5 drives the scraper 106 to move, thereby scraping the inner surface of the tank 101. The auxiliary component facilitates the discharge of cell raw materials from the tank 101. The temperature control component on the tank 101 controls the internal temperature of the tank 101, ensuring that the cells are cultured at the optimal temperature. During the discharge process after cell culture, the moving ring 105 and the scraper 106 are arranged in the inner cavity of the tank 101. The pushing component drives the moving ring 105 to move in the inner cavity of the tank 101, causing the moving ring 105 to drive the scraper 106 to scrape the raw materials on the inner surface of the tank 101, preventing cells from adhering to the inner wall of the tank 101, thereby improving the productivity of cell culture.

[0024] Secondly, the positioning cylinder 107 is fixedly connected to the upper cover 104 and located on one side of the upper cover 104; the push rod 108 is fixedly connected to the moving ring 105 and slidably connected to the positioning cylinder 107; the force-applying rod 109 is fixedly connected to the push rod 108 and located on one side of the push rod 108. The positioning cylinder 107 is a tubular structure, and the positioning cylinder 107 is disposed through the left side of the upper cover 104. The positioning cylinder 107 allows the interior of the tank 101 to communicate with the outside. The push rod 108 is located inside the positioning cylinder 107. The bottom end of the push rod 108 is fixed to the left side of the upper surface of the moving ring 105. The top end of the push rod 108 is fixed with the force-applying rod 109, so that the force-applying rod 109 can drive the push rod 108 to move along the direction of the positioning cylinder 107, thereby causing the push rod 108 to drive the moving ring 105 to move.

[0025] Meanwhile, the conical barrel 110 is fixedly connected to the tank body 101 and located at the bottom of the inner cavity of the tank body 101; the limiting ring 111 is fixedly connected to the tank body 101 and located on one side of the tank body 101. The conical barrel 110 is located at the bottom of the inner cavity of the tank body 101. The conical barrel 110 has a conical structure, with the smaller diameter end of the conical barrel 110 facing downwards and communicating with the discharge port at the bottom of the tank body 101, so that the material can be discharged after cell culture. During the process, the conical barrel 110 facilitates cell discharge. The limiting ring 111 is fixed on the inner surface of the tank 101. The limiting ring 111 is located above the conical barrel 110. The surface of the limiting ring 111 is provided with through holes. After the scraper 106 scrapes the cells off the inner surface of the tank 101, the cell raw material can enter the discharge port at the bottom of the tank 101 through the through holes on the limiting ring 111 for discharge.

[0026] In addition, the heating element 112 is disposed in the mounting groove formed by the outer wall and the inner cavity of the tank 101; the temperature display 113 is disposed on the tank 101, and the mounting groove is formed between the outer wall and the inner wall of the tank 101. The heating element 112 is located in the mounting groove. When the heating element 112 is activated, the inside of the tank 101 can be heated, thereby enabling the temperature inside the tank 101 to be adjusted according to the cell characteristics. The temperature display 113 is disposed on the outer surface of the tank 101. The temperature display 113 is located above the endoscope 102. The temperature display 113 is used to display the temperature inside the tank 101, which facilitates the operator to observe the temperature inside the tank 101 intuitively.

[0027] When using the fully automated fermenter for sterilized and homogeneous cell culture media according to this embodiment, during the discharge process after cell culture, a moving ring 105 and a scraper 106 are provided in the inner cavity of the tank body 101. The moving ring 105 is driven to move in the inner cavity of the tank body 101 by the pushing component, so that the moving ring 105 drives the scraper 106 to scrape off the raw materials on the inner surface of the tank body 101, avoiding cell adhesion to the inner wall of the tank body 101, thereby improving the productivity of cell culture.

[0028] The above-disclosed embodiments are merely one or more preferred embodiments of this application and should not be construed as limiting the scope of this application. Those skilled in the art can understand that all or part of the processes for implementing the above embodiments and equivalent changes made in accordance with the claims of this application still fall within the scope of this application.

Claims

1. A fully automated fermenter for sterilizing and homogenizing cell culture medium, comprising a tank body, a sight glass, a pH meter, and a top cover, wherein the sight glass and the pH meter are disposed on the front surface of the tank body, the pH meter being located below the sight glass, and the top cover being detachably connected to the tank body and located on one side of the tank body, characterized in that: It also includes a scraping device; The scraping device includes a movable ring, a scraper, a pushing component, an auxiliary component, and a temperature control component. The movable ring is slidably connected to the tank and located on one side of the tank. The scraper is fixedly connected to the movable ring and located on one side of the movable ring. The pushing component is disposed on the movable ring. The auxiliary component is disposed on the tank. The temperature control component is disposed on the tank.

2. The fully automated fermenter for sterilized and homogeneous cell culture medium as described in claim 1, characterized in that: The pushing assembly includes a positioning cylinder and a push rod. The positioning cylinder is fixedly connected to the upper cover and located on one side of the upper cover. The push rod is fixedly connected to the moving ring and slidably connected to the positioning cylinder.

3. The fully automated fermenter for sterilized and homogeneous cell culture medium as described in claim 2, characterized in that: The pushing assembly also includes a force-applying rod, which is fixedly connected to the push rod and located on one side of the push rod.

4. The fully automated fermenter for sterilized and homogeneous cell culture medium as described in claim 1, characterized in that: The auxiliary component includes a conical barrel and a limiting ring. The conical barrel is fixedly connected to the tank body and is located at the bottom of the inner cavity of the tank body; the limiting ring is fixedly connected to the tank body and is located on one side of the tank body.

5. The fully automated fermenter for sterilized and homogeneous cell culture medium as described in claim 1, characterized in that: The temperature control component includes a heating element and a temperature display. The heating element is disposed in a mounting groove formed by the outer wall of the tank and the inner cavity; the temperature display is disposed on the tank.

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