Sample collection device based on aquatic product safety

By designing a sample collection device with threaded connections for the pre-filter assembly, adapter bottle, filter assembly, and collection bottle, the problems of unstable connections and detection were solved, achieving efficient and stable virus collection and applicability to multiple samples.

CN224258626UActive Publication Date: 2026-05-19CHINESE ACAD OF INSPECTION & QUARANTINE +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Utility models(China)
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Filing Date
2025-04-25
Publication Date
2026-05-19

AI Technical Summary

Technical Problem

Existing aquatic animal virus collection devices suffer from problems such as easily detached connecting tubes, complex structures, and difficulty in accurately detecting viruses in water using existing detection methods. Furthermore, the filter components have limited applicability.

Method used

A sample collection device was designed, comprising a pre-filtration component, an adapter bottle, a filter component, and a collection bottle. The components are stabilized by threaded connections, and a vacuum generator is used to provide a negative pressure environment to achieve efficient virus collection.

Benefits of technology

It achieves stability and accuracy in virus collection, simplifies the device structure, is suitable for collecting various viruses, and improves the reliability of detection.

✦ Generated by Eureka AI based on patent content.

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Abstract

The utility model relates to a sample collecting device based on aquatic product safety, which comprises a pre-filtering assembly, a first sampling assembly, a second sampling assembly, a second sampling assembly and a third sampling assembly, and is characterized in that the pre-filtering assembly comprises a first bottle body and a first filter screen; the transfer bottle comprises a second bottle body, the second bottle body is provided with a second inlet and a second outlet, and the outer wall of the first bottle body can abut against the upper end of the second inlet; the filtering assembly comprises a third bottle body and a filtering part, the third bottle body comprises an upper bottle body and a lower bottle body, the upper bottle body is provided with a third inlet, the lower bottle body is provided with a third outlet, and the filtering part is arranged on the lower bottle body; the collecting bottle comprises a fourth bottle body with a fourth inlet; the second outlet is in threaded connection with the third inlet; the third outlet is in threaded connection with the fourth inlet; and the vacuum generator is connected with the fourth bottle body. The first bottle body and the third bottle body do not need to be supported and lifted by a bracket, and the sample collection device is simple in structure. The transfer bottle, the third bottle body and the collecting bottle are connected through threads, and every two connected bottle bodies are not prone to being disconnected.
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Description

Technical Field

[0001] This utility model relates to the field of aquatic product safety control technology, and in particular to a sample collection device based on aquatic product safety. Background Technology

[0002] Aquatic animal viruses are a class of microorganisms that threaten the safety of aquatic products. When aquatic animals, such as fish, are infected with aquatic animal viruses, their internal organs often contain high concentrations of the virus. However, because water is the primary abiotic medium for the transmission of aquatic animal viruses, these viruses can survive in water for extended periods; for example, carp spring viremia virus can survive in water for more than four weeks. Therefore, detecting aquatic animal viruses in water can help block their transmission and protect the safety of aquatic products.

[0003] The detection of viruses in aquatic animals has a wide range of applications, such as...

[0004] 1. Before releasing water into aquatic animal breeding facilities, it is necessary to test the water for aquatic animal viruses in advance to avoid releasing water containing aquatic animal viruses into the breeding facilities, which could threaten the safety of aquatic animals.

[0005] 2. When it is necessary to release fish fry or other aquatic animals into a breeding site, it is necessary to test whether the fish fry or other aquatic animals contain aquatic animal viruses in advance. In order not to harm the aquatic animals, it is often necessary to test whether the water in which the fish fry or other aquatic animals live contains aquatic animal viruses.

[0006] 3. When exporting aquatic animals, a report on the safety of the aquatic animals is required. In this case, the presence of aquatic animal viruses in the water can also be determined by testing.

[0007] However, due to the low concentration of aquatic animal viruses in the aquatic environment, existing detection methods are insufficient to accurately detect the presence of these viruses in water. Therefore, how to collect aquatic animal viruses from water has become a key technology for ensuring the safety of aquatic animals. Existing technologies include devices for collecting aquatic animal viruses, but these devices require a connecting pipe between the filter assembly and the collection device. During vacuum filtration, this connecting pipe is prone to detachment, leading to virus collection failure. Furthermore, existing technologies require the filter assembly to be raised by a support to allow water to pass through the filter screen, making the device structure complex. Additionally, existing technologies include collection and detection devices that determine the presence of aquatic animal viruses through the specific binding of antigens and antibodies. While these have high specificity, they are only suitable for collecting single viruses.

[0008] Therefore, in order to overcome the shortcomings of existing technologies, there is a need to provide a sample collection device based on the safety of aquatic products. Utility Model Content

[0009] The purpose of this invention is to propose a sample collection device based on the safety of aquatic products, so as to solve at least one of the above-mentioned technical problems.

[0010] To achieve the above objectives, this utility model provides a sample collection device based on aquatic product safety, comprising:

[0011] A pre-filtration assembly includes a first bottle and a first filter screen, the first filter screen being connected to the first bottle, the upper end of the first bottle having a first inlet and the lower end having a first outlet;

[0012] The adapter bottle includes a second bottle body, the upper end of which has a second inlet and the lower end of which has a second outlet. The first bottle body can be inserted into the second inlet, and the outer wall of the first bottle body can abut against the upper end of the second inlet.

[0013] A filtering assembly includes a third bottle and a filtering section. The third bottle includes an upper bottle and a lower bottle that are connected to each other. The upper bottle is located above the lower bottle and is detachably connected to the lower bottle. The upper end of the upper bottle has a third inlet, and the lower end of the lower bottle has a third outlet. The filtering section is detachably disposed in the lower bottle and is capable of retaining the sample to be tested.

[0014] A collection bottle includes a fourth bottle body, the upper end of which has a fourth inlet; a second outlet is threadedly connected to the third inlet, and the third outlet is threadedly connected to the fourth inlet;

[0015] A vacuum generator is connected to the fourth bottle.

[0016] Optionally, one of the upper bottle body and the lower bottle body has an external thread, and the other has an internal thread. The upper bottle body and the lower bottle body are connected by the internal thread and the external thread.

[0017] Optionally, the filtration section includes a second filter screen and a filter membrane, the filter membrane covering the second filter screen, and the second filter screen being detachably connected to the lower bottle body.

[0018] Optionally, the inner wall of the lower bottle is provided with an annular support portion, and the second filter screen is disposed on the annular support portion.

[0019] Optionally, the inner wall of the lower bottle body is provided with an annular groove, and the bottom of the annular groove forms the annular support portion; or

[0020] The inner wall of the lower bottle is connected to an annular protrusion, which forms the annular support portion.

[0021] Optionally, the filter membrane is a cation exchange filter membrane.

[0022] Optionally, the cation exchange membrane is a cation nitrocellulose membrane.

[0023] Optionally, the pre-filter assembly further includes a filter layer disposed on the first filter screen.

[0024] Optionally, the filter layer is a glass fiber membrane.

[0025] Optionally, the first bottle, the adapter bottle, the third bottle and / or the collection bottle are made of polycarbonate.

[0026] Optionally, the collection bottle further includes a connection interface, which is connected to the fourth bottle body, and the vacuum generator is connected to the connection interface.

[0027] Optionally, the first bottle body includes a constricted portion, the outer diameter of which gradually increases from bottom to top, the lower end of which has a first outlet, the constricted portion being able to be inserted into the second inlet, and the outer side of which can abut against the upper end of the second inlet.

[0028] As can be seen from the above, the technical solution provided by this utility model, when using a sample collection device based on aquatic product safety, places the filter section in the lower bottle body, with the upper bottle body threadedly connected to the lower bottle body. The collection bottle is threadedly connected to the third bottle body, and the third bottle body is threadedly connected to the adapter bottle. Then, the first bottle body is placed at the second inlet, and the vacuum generator is connected to the connection interface. The vacuum generator is turned on, and the liquid to be filtered is poured into the first bottle body through the first inlet. After passing through the first filter screen and the filter section, the liquid flows into the collection bottle. Samples to be collected, such as viruses, are trapped on the filter section. The upper and lower bottle bodies are disassembled, the filter section is removed, and the samples on the filter section can be collected.

[0029] The pre-filtration assembly pre-filters the liquid, trapping larger impurities and preventing blockage of the filter section. The outer wall of the first bottle abuts against the upper end of the second inlet, thus the second bottle stably supports the first bottle. Furthermore, if the first bottle becomes clogged and cannot pre-filter the liquid, it can be removed, allowing the second bottle to continue supplying liquid to the third bottle, ensuring successful filtration. The vacuum generator provides a negative pressure environment for the fourth bottle, promoting smooth liquid filtration.

[0030] The first bottle is placed directly on the second opening of the second bottle, which supports the first. The third bottle is threadedly connected to the collection bottle. No support bracket is needed to elevate the first and third bottles, resulting in a simple sample collection device designed for aquatic product safety. The adapter bottle, third bottle, and collection bottle are sequentially threaded together, ensuring a stable connection and preventing breakage between connected bottles, thus guaranteeing successful sampling. Attached Figure Description

[0031] Figure 1 This is a schematic diagram of the structure of the sample collection device based on aquatic product safety provided in this embodiment of the utility model;

[0032] Figure 2 This is a cross-sectional view of the sample collection device (removing the filter layer and filter membrane) based on the safety of aquatic products provided in this embodiment of the utility model;

[0033] Figure 3 This is an exploded view of the sample collection device for aquatic product safety provided in this embodiment of the present invention;

[0034] Figure 4 yes Figure 2 A magnified view of a section at point A in the middle;

[0035] Figure 5 This is an exploded view of the filter assembly provided in an embodiment of this utility model;

[0036] Figure 6 yes Figure 2 A magnified view of a section at point B in the middle;

[0037] Figure 7 This is a schematic diagram of another annular support portion provided in an embodiment of the present utility model.

[0038] In the picture:

[0039] 1. Pre-filtration assembly; 11. First bottle body; 111. Straight section; 112. Neck section; 12. First filter screen; 13. First inlet; 14. First outlet;

[0040] 2. Transfer bottle; 21. Second bottle body; 22. First cylindrical mouth ring; 23. Second inlet; 24. Second outlet;

[0041] 3. Filter assembly; 31. Third bottle body; 311. Upper bottle body; 312. Lower bottle body; 3121. Annular groove; 3122. Annular protrusion; 313. Second cylindrical inlet ring; 314. Third cylindrical inlet ring; 32. Filter section; 321. Second filter screen; 322. Filter membrane; 33. Third inlet; 34. Third outlet;

[0042] 4. Collection bottle; 41. Fourth bottle body; 42. Fourth cylindrical mouth ring; 43. Connection interface; 44. Fourth inlet. Detailed Implementation

[0043] The technical solution of this utility model will be further described below with reference to the accompanying drawings and specific embodiments. It should be understood that the specific embodiments described herein are merely for explaining this utility model and not for limiting it. Furthermore, it should be noted that, for ease of description, only the parts related to this utility model are shown in the accompanying drawings, not all of them.

[0044] This utility model defines certain directional terms. Unless otherwise stated, the directional terms used, such as "up", "down", "left", "right", "inner", and "outer", are used for ease of understanding and therefore do not constitute a limitation on the scope of protection of this utility model.

[0045] In this invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can include direct contact between the first and second features, or contact between the first and second features through another feature between them. Furthermore, "above," "over," and "on top" of the second feature includes the first feature directly above or diagonally above the second feature, or simply indicates that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature includes the first feature directly below or diagonally below the second feature, or simply indicates that the first feature is at a lower horizontal level than the second feature.

[0046] In the description of this utility model, unless otherwise explicitly specified and limited, the terms "connected," "linked," and "fixed" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model based on the specific circumstances.

[0047] This embodiment provides a sample collection device based on aquatic product safety, used to collect samples of bacteria, viruses, etc. in water, especially viruses, but not limited to these, and can also be used to collect samples from other liquids.

[0048] like Figures 1-3 As shown, the sample collection device based on aquatic product safety provided in this embodiment includes a pre-filtration component 1, a transfer bottle 2, a filter component 3, a collection bottle 4, and a vacuum generator (not shown in the figure).

[0049] The pre-filtration component 1 includes a first bottle body 11 and a first filter screen 12. The first filter screen 12 is connected inside the first bottle body 11. The upper end of the first bottle body 11 has a first inlet 13 and the lower end has a first outlet 14.

[0050] The adapter bottle 2 includes a second bottle body 21, with a second inlet 23 at the upper end and a second outlet 24 at the lower end. The first bottle body 11 can be inserted into the second inlet 23, and the outer wall of the first bottle body 11 can abut against the upper end of the second inlet 23.

[0051] The filter assembly 3 includes a third bottle 31 and a filter section 32. The third bottle 31 includes an upper bottle 311 and a lower bottle 312 that are connected to each other. The upper bottle 311 is located on the upper side of the lower bottle 312 and is detachably connected to the lower bottle 312. The upper end of the upper bottle 311 has a third inlet 33, and the lower end of the lower bottle 312 has a third outlet 34. The filter section 32 is detachably disposed on the lower bottle 312 and can retain the sample to be tested.

[0052] The collection bottle 4 includes a fourth bottle body 41, with a fourth inlet 44 at its upper end. A second outlet 24 is threadedly connected to a third inlet 33, and a third outlet 34 is threadedly connected to the fourth inlet 44. A vacuum generator is connected to the fourth bottle body 41 to provide a negative pressure environment for the fourth bottle body 41.

[0053] Optionally, the collection bottle 4 also includes a connection interface 43, which is connected to the fourth bottle body 41, and the vacuum generator is connected to the connection interface 43. Exemplarily, the connection interface 43 is integrally formed with the fourth bottle body 41. The connection interface 43 can be connected to the vacuum generator via a tubing or the like. The vacuum generator is existing technology and will not be described in detail here.

[0054] When using the sample collection device based on aquatic product safety, the filter section 32 is installed in the lower bottle body 312, and the upper bottle body 311 is threadedly connected to the lower bottle body 312. The collection bottle 4 is threadedly connected to the third bottle body 31, and the third bottle body 31 is threadedly connected to the adapter bottle 2. Then, the first bottle body 11 is placed in the second inlet 23, and the vacuum generator is connected to the connection interface 43. The vacuum generator is turned on, and the liquid to be filtered (e.g., water, hereinafter referred to as sample water) is poured into the first bottle body 11 through the first inlet 13. After passing through the first filter screen 12 and the filter section 32, the liquid flows into the collection bottle 4. The sample to be collected, such as a virus, is trapped on the filter section 32. The upper bottle body 311 and the lower bottle body 312 are disassembled, the filter section 32 is removed, and the sample on the filter section 32 can be collected.

[0055] The pre-filtration assembly 1 can pre-filter the sample water, trapping larger impurities and preventing blockage of the filter section 32. The outer wall of the first bottle 11 can abut against the upper end of the second inlet 23, thus the second bottle 21 can stably support the first bottle 11. Furthermore, if the first bottle 11 becomes blocked and cannot pre-filter the sample water, it can be removed, and the second bottle 21 can continue to supply sample water to the third bottle 31, ensuring smooth filtration. The vacuum generator can provide a negative pressure environment for the fourth bottle 41, promoting smooth filtration of the sample water.

[0056] In this embodiment, the first bottle 11 is directly placed on the second opening of the second bottle 21, with the second bottle 21 serving to support the first bottle 11. The third bottle 31 and the collection bottle 4 are threaded together, eliminating the need for a support bracket to elevate the first bottle 11 and the third bottle 31. This results in a simple structure for the sample collection device based on aquatic product safety. The adapter bottle 2, the third bottle 31, and the collection bottle 4 are sequentially connected by threads, ensuring a stable connection and preventing the connection between the two bottles from easily breaking, thus guaranteeing successful sampling.

[0057] like Figure 2 and Figure 3 As shown, the upper end of the first bottle 11 is open, forming a first inlet 13. The upper end of the second bottle 21 is also open, forming a second inlet 23.

[0058] Optionally, a first cylindrical mouth ring 22 is connected to the lower end of the second bottle body 21, forming a second outlet 24. A second cylindrical mouth ring 313 is connected to the upper end of the upper bottle body 311, forming a third inlet 33. A third cylindrical mouth ring 314 is connected to the lower end of the lower bottle body 312, forming a third outlet 34. A fourth cylindrical mouth ring 42 is connected to the upper end of the fourth bottle body 41, forming a fourth inlet 44. The cylindrical mouth rings are provided with internal or external threads to achieve threaded connection between adjacent cylindrical mouth rings.

[0059] For example, the first cylindrical ring 22 has an internal thread, and the second cylindrical ring 313 has an external thread, and the first cylindrical ring 22 and the second cylindrical ring 313 are threaded together. The third cylindrical ring 314 has an internal thread, and the fourth cylindrical ring 42 has an external thread, and the third cylindrical ring 314 and the fourth cylindrical ring 42 are threaded together.

[0060] The first bottle 11, the transfer bottle 2, the third bottle 31, and / or the collection bottle 4 are made of polycarbonate. Polycarbonate has high transparency, allowing operators to clearly see the filtration process and promptly address any unexpected issues such as blockages. Polycarbonate also possesses good chemical resistance and high-temperature resistance, making it suitable for most filtration needs. The polycarbonate transfer bottle 2, the third bottle 31, and the collection bottle 4 are sealed via threaded connections, eliminating the need for sealing rings and simplifying the structure of the sample collection device for aquatic product safety. The threaded connection between the transfer bottle 2, the third bottle 31, and the collection bottle 4 prevents air leakage. During vacuum generation, a negative pressure environment is maintained in the third bottle 31 (especially the space below the filter section 32) and the collection bottle 4, promoting smooth filtration of the sample water.

[0061] like Figure 2 and Figure 4 As shown, the first bottle body 11 includes a constricted portion 112, the outer diameter of which gradually increases from bottom to top, the lower end of the constricted portion 112 has a first outlet 14, the constricted portion 112 can be inserted into the second inlet 23, and the outer side of the constricted portion 112 can abut against the upper end of the second inlet 23.

[0062] The outer diameter of the constriction portion 112 gradually increases from bottom to top, thereby accommodating second inlets 23 of different sizes and ensuring that the outer side of the constriction portion 112 can abut against the insertion of the second inlet 23, reducing the dimensional accuracy requirements of the second inlet 23, while stably supporting the first bottle body 11.

[0063] Optionally, the first bottle body 11 may also include a straight cylindrical portion 111, which is connected above the constricted portion 112.

[0064] like Figure 5 As shown, one of the upper bottle body 311 and the lower bottle body 312 has an external thread, and the other has an internal thread. The upper bottle body 311 and the lower bottle body 312 are connected by the internal and external threads. For example, the upper bottle body 311 has an external thread, and the lower bottle body 312 has an internal thread. The upper bottle body 311 and the lower bottle body 312 are easy to install and remove, and the connection is stable.

[0065] The filtration section 32 includes a second filter screen 321 and a filter membrane 322. The filter membrane 322 covers the second filter screen 321, and the second filter screen 321 is detachably connected to the lower bottle body 312. Optionally, the filter membrane 322 can be bonded to the second filter screen 321. In other optional embodiments, the filter membrane 322 can be placed directly on the second filter screen 321.

[0066] Optionally, the filter membrane 322 is a cation exchange filter membrane. Cation exchange filter membranes readily adsorb samples containing anions and can adsorb samples smaller than their pore size, thus making it easier for the filter membrane 322 to collect samples and resulting in a higher sample collection rate. Optionally, the pore size of the cation exchange filter membrane is 0.45 micrometers, but it is not limited to this.

[0067] In a typical aquatic environment, most viruses carry a negative charge on their surface. In addition, some bacteria also carry a negative charge. Therefore, the sample collection device based on aquatic product safety provided in this embodiment can collect a wide range of samples.

[0068] Optionally, the cation exchange filtration membrane is a cation exchange nitrocellulose membrane. For example, a nitrocellulose membrane treated with aluminum trichloride, where trivalent chloride ions are adsorbed. Specifically, the nitrocellulose membrane is immersed in a 250 mM aluminum trichloride solution, allowed to stand at room temperature for 10-30 minutes, and then removed and air-dried. It should be understood that mM is an abbreviation for millimoles per liter, representing the concentration unit of the solution. Cation exchange nitrocellulose membranes are existing technology, and their processing technology will not be described in detail here.

[0069] like Figure 6 and Figure 7 As shown, the inner wall of the lower bottle body 312 is provided with an annular support portion, and the filter portion 32 is disposed on the annular support portion. Specifically, the second filter screen 321 is disposed on the annular support portion to achieve a detachable connection between the second filter screen 321 and the lower bottle body 312. The annular support portion can support the entire edge of the filter portion 32, thereby improving the stability of the filter portion 32.

[0070] like Figure 6 As shown, an annular protrusion 3122 is connected to the inner wall of the lower bottle body 312, forming an annular support portion. The support portion has a simple structure and can be connected to the lower bottle body 312 by means of bonding or ultrasonic welding.

[0071] like Figure 7 As shown, in other optional embodiments, an annular groove 3121 is formed at the upper end of the lower bottle body 312, and the bottom of the annular groove 3121 forms an annular support portion. The annular groove 3121 can be formed during the injection molding of the lower bottle body 312, which is convenient and efficient in molding.

[0072] The first bottle body 11 and the first filter screen 12 can be integrally formed by injection molding.

[0073] Optionally, the pre-filtration assembly 1 may further include a filter layer (not shown in the figure), which is disposed on the first filter screen 12. The diameter of the filter layer may be the same as the diameter of the first filter screen 12, thereby ensuring that all sample water passes through the filter layer. The pores of the first filter screen 12 may be too large to meet the pre-filtration requirements; the filter layer can filter out smaller impurities.

[0074] Optionally, the filter layer is a glass fiber membrane or glass fiber filter paper. Glass fiber membranes and glass fiber filter paper are existing technologies, and their processing methods will not be described in detail here.

[0075] For example, when using the sample collection device based on aquatic product safety provided in this embodiment, 500ml (not limited to 500ml, which can be determined according to the concentration of the sample to collect a sufficient amount of sample) of sample water is filtered through the sample collection device based on aquatic product safety. Then, it is rinsed with 100ml of 0.5mM H2SO4 (pH 3.0) to remove residual aluminum ions on the filter membrane 322. Then, the upper bottle 311 is rotated to separate the upper bottle 311 and the lower bottle 312. Subsequently, the filter membrane 322 is removed and placed in 20ml of 1.0mM NaOH (pH 10.8) solution. After gently stirring for 2 minutes, the liquid is collected to wash away the viruses captured on the filter membrane 322.

[0076] Although the present invention has been described in detail above with general descriptions, specific embodiments, and experiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention fall within the scope of protection claimed by the present invention.

Claims

1. A sample collection device based on aquatic product safety, characterized in that, include: The pre-filtration assembly (1) includes a first bottle body (11) and a first filter screen (12), the first filter screen (12) being connected inside the first bottle body (11), the upper end of the first bottle body (11) having a first inlet (13) and the lower end having a first outlet (14); The adapter bottle (2) includes a second bottle body (21), the upper end of the second bottle body (21) has a second inlet (23) and the lower end has a second outlet (24), the first bottle body (11) can be inserted into the second inlet (23), and the outer wall of the first bottle body (11) can abut against the upper end of the second inlet (23); The filter assembly (3) includes a third bottle (31) and a filter section (32). The third bottle (31) includes an upper bottle (311) and a lower bottle (312) that are connected to each other. The upper bottle (311) is located on the upper side of the lower bottle (312) and is detachably connected to the lower bottle (312). The upper end of the upper bottle (311) has a third inlet (33), and the lower end of the lower bottle (312) has a third outlet (34). The filter section (32) is detachably disposed on the lower bottle (312) and the filter section (32) is capable of retaining the sample to be tested. The collection bottle (4) includes a fourth bottle body (41), the upper end of which has a fourth inlet (44); the second outlet (24) is threadedly connected to the third inlet (33), and the third outlet (34) is threadedly connected to the fourth inlet (44); A vacuum generator is connected to the fourth bottle (41).

2. The sample collection device based on aquatic product safety according to claim 1, characterized in that, One of the upper bottle body (311) and the lower bottle body (312) has an external thread, and the other has an internal thread. The upper bottle body (311) and the lower bottle body (312) are connected by the internal thread and the external thread.

3. The sample collection device based on aquatic product safety according to claim 1, characterized in that, The filtration section (32) includes a second filter screen (321) and a filter membrane (322), the filter membrane (322) covering the second filter screen (321), and the second filter screen (321) being detachably connected to the lower bottle body (312).

4. The sample collection device based on aquatic product safety according to claim 3, characterized in that, The inner wall of the lower bottle body (312) is provided with an annular support portion, and the second filter screen (321) is disposed on the annular support portion.

5. The sample collection device based on aquatic product safety according to claim 4, characterized in that, The inner wall of the lower bottle body (312) is provided with an annular groove (3121), and the bottom of the annular groove (3121) forms the annular support part; or The inner wall of the lower bottle body (312) is connected to an annular protrusion (3122), which forms the annular support portion.

6. The sample collection device based on aquatic product safety according to claim 3, characterized in that, The filter membrane (322) is a cation exchange filter membrane.

7. The sample collection device based on aquatic product safety according to claim 6, characterized in that, The cation filtration membrane is a cation nitrocellulose membrane.

8. The sample collection device based on aquatic product safety according to claim 1, characterized in that, The pre-filter assembly (1) further includes a filter layer disposed on the first filter screen (12).

9. The sample collection device based on aquatic product safety according to claim 8, characterized in that, The filter layer is a glass fiber membrane.

10. The sample collection device based on aquatic product safety according to claim 1, characterized in that, The first bottle body (11), the adapter bottle (2), the third bottle body (31), and / or the collection bottle (4) are made of polycarbonate, and / or The collection bottle (4) further includes a connection interface (43), which is connected to the fourth bottle body (41), and the vacuum generator is connected to the connection interface (43), and / or The first bottle body (11) includes a constricted portion (112), the outer diameter of which gradually increases from bottom to top, the lower end of which has a first outlet (14), the constricted portion (112) can be inserted into the second inlet (23), and the outer side of which can abut against the upper end of the second inlet (23).