A fixation and perfusion device for rat and mouse experiments

Abstract

This utility model relates to a fixation and perfusion device for rat and mouse experiments, comprising: a mouse fixation plate, a temperature-controlled pad, a fixation patch, a fixation rod, and a perfusion needle. The mouse fixation plate is mounted on an operating table; the temperature-controlled pad is mounted on the upper surface of the mouse fixation plate to maintain the mouse's body temperature; the fixation patch is positioned near the temperature-controlled pad to fix the mouse's limbs; the fixation rod is mounted on the mouse fixation plate to cooperate with the perfusion needle; and the perfusion needle is mounted on the fixation rod for perfusion of the mouse's heart. Its advantages are: this utility model has a simple structure and is easy to operate. Through the fixation patch and multiple threaded slots, it can fix the limbs and head of mice of different sizes; thus facilitating experimental operations for heart perfusion in rats and mice, improving the success rate of experiments, expanding the research scope, and saving experimental costs.

Description

Technical Field

[0001] This invention belongs to the field of experimental technology for mice and rats, and relates to a fixed perfusion device for experiments on mice and rats. Background Technology

[0002] In medical research, the basic steps for morphological studies include perfusion fixation of the heart in experimental animals (mice and rats) followed by the collection of brain tissue and other organ samples.

[0003] Existing mouse cardiac perfusion fixation devices generally involve fixing mice and rats to a work platform and then using an infusion device for perfusion fixation. During the fixation process, nails are often driven into the table and rubber bands or tape are used to secure the mice and rats. In addition to being uncomfortable and not very secure, this fixation method can easily lead to the death of the animals due to hypothermia during surgery.

[0004] Furthermore, problems during fixation can affect the experimental process and results during surgical procedures such as mouse heart perfusion. Therefore, a good fixation method can provide stable experimental data support for mouse heart perfusion and improve the success rate of the experiment.

[0005] Therefore, in summary, there is an urgent need for a method that can stably fix the limbs and head of mice and provide a certain degree of constant temperature, thereby solving the problems of low comfort after fixation of mice and rats, hypothermia during surgery, and inability to stably perfuse the mouse heart. Utility Model Content

[0006] The purpose of this invention is to solve the above-mentioned problems in the prior art and to provide a fixed perfusion device for mouse and rat experiments.

[0007] To achieve the above objectives, the technical solution adopted by this utility model is as follows:

[0008] A fixation and perfusion device for rat and mouse experiments includes: a mouse fixation plate disposed on an operating table; a temperature-controlled pad disposed on the upper surface of the mouse fixation plate to maintain the mouse's body temperature; a fixation patch disposed near the temperature-controlled pad to fix the mouse's hands and feet; a fixation rod disposed on the mouse fixation plate to cooperate with a perfusion needle; and a perfusion needle disposed on the fixation rod for perfusion of the mouse's heart.

[0009] Preferably, the upper surface of the mouse fixation plate is provided with a threaded groove, and a fixing bolt is correspondingly provided in the threaded groove; a fixing rope for fixing the mouse's head is also wrapped around the fixing bolt.

[0010] Preferably, the threaded slots are provided in multiple locations and are arranged at equal intervals in the transverse direction.

[0011] Preferably, four fixing stickers are provided, and they are symmetrically arranged at the four corners near the constant temperature pad.

[0012] Preferably, the fixing patch is an adjustable fixing patch, comprising an integrally formed fixing patch, a first adjusting fixing band, and a second adjusting fixing band; the two ends of the fixing patch are respectively fixedly connected to the first adjusting fixing band and the second adjusting fixing band; and a groove is provided on the first adjusting fixing band, the first adjusting fixing band passing through the groove on the second adjusting fixing band to clamp and fix the mouse's hands and feet placed on the fixing patch.

[0013] Preferably, the fixation patch is fixedly mounted on the mouse fixation plate; the first adjustment fixation strap and the second adjustment fixation strap are respectively attached and fixed to the mouse fixation plate by means of hook and loop fasteners.

[0014] Preferably, the fixing rod has a spiral structure and is flexible; the bottom end of the fixing rod is fixedly connected to the mouse fixation plate, and its head end is also provided with a fixing clip for fixing the infusion needle, wherein the infusion needle is provided in the fixing clip.

[0015] Preferably, the fixing clip includes a fixing sleeve and a fixing component disposed on the fixing sleeve.

[0016] Preferably, the infusion needle includes an infusion needle tip and an infusion conduit, one end of which is connected to the infusion needle tip and the other end is connected to a three-way valve; wherein, the three-way valve is used to connect to an external pumping system.

[0017] Due to the adoption of the above technical solution, the beneficial effects obtained by this utility model include:

[0018] 1. This utility model has a simple structure and is easy to operate. Through the setting of fixing stickers and multiple threaded slots, it can fix the limbs and head of mice of different sizes; thereby facilitating the experimental operation of cardiac perfusion in mice and rats, improving the success rate of the experiment, expanding the research scope, and saving experimental costs.

[0019] 2. The constant temperature pad in this invention can provide good body temperature maintenance for mice;

[0020] 3. In this utility model, the injection fixing rod can accurately fix the position of the injection needle, and the external injection pump connected to its three-way valve can inject quantitatively and at a constant speed to achieve the desired effect. Attached Figure Description

[0021] Figure 1 This is a schematic diagram of the fixed perfusion device of this utility model used in experiments with mice and rats.

[0022] Figure 2 This is a front view of the fixed perfusion device of this utility model used in experiments with mice and rats.

[0023] Figure 3 This is a partial structural diagram of the mouse fixation plate and threaded slot of this utility model.

[0024] Figure 4 This is a partial structural diagram of the fixing rod and injection needle of this utility model.

[0025] Figure 5 This is a partial structural diagram of the fixing sticker of this utility model.

[0026] Figure 6 These are partial implementation diagrams of the fixing sticker of this utility model.

[0027] The attached figures are labeled as follows:

[0028] 1. Mouse restraint plate; 11. Threaded slot; 12. Fixing bolt; 13. Fixing rope;

[0029] 2. Thermostatic pad; 21. Display; 22. On / off switch; 23. Temperature adjustment button;

[0030] 3. Fixing adhesive; 31. Fixing application; 32. First adjusting and fixing strap;

[0031] 33. Second adjusting and fixing belt; 34. Groove;

[0032] 4. Fixing rod; 41. Fixing clip; 42. Fixing sleeve; 43. Fixing assembly; 44. Opening;

[0033] 5. Infusion needle; 51. Infusion needle tip; 52. Infusion catheter; 53. Three-way valve. Detailed Implementation

[0034] Please see the appendix Figure 1 , Figure 2As shown, this utility model mainly provides a fixation and infusion device for mouse and rat experiments, including: a mouse fixation plate 1, a constant temperature pad 2, a fixation patch 3, a fixation rod 4, and an infusion needle 5. The mouse fixation plate 1 is set on the operating table; the constant temperature pad 2 is set on the upper surface of the mouse fixation plate 1 to maintain the mouse's body temperature; the constant temperature pad 2 includes a heating plate, a temperature sensor, a display 21, an on / off switch 22, and a temperature adjustment button 23. The heating plate and the temperature sensor are both installed in the middle of the upper surface of the mouse fixation plate (not clearly shown in the figure for simplicity), and the display 21, the on / off switch 22, and the temperature adjustment button 23 are all installed at the corners of the upper surface of the mouse fixation plate. The heating plate, the temperature sensor, the display 21, the on / off switch 22, and the temperature adjustment button 23 are all electrically connected through wires 24. The heating plate, temperature sensor, display 21, on / off switch 22, and temperature adjustment button 23 are all existing structures and technologies. The on / off switch 22 can control the opening and closing of the heating plate, the temperature adjustment button 23 can control the temperature of the heating plate, and the display 21 and the temperature sensor can display the heat generated by the heating plate. That is, the heating plate can provide heat to the mice during the operation, so as to prevent the mice from hypothermia during the operation.

[0035] like Figure 3 As shown, in this embodiment, the upper surface of the mouse fixation plate 1 is provided with a plurality of threaded slots 11 arranged laterally at equal intervals, and a fixing bolt 12 is correspondingly provided in the threaded slot 11; wherein, a fixing rope 13 for fixing the mouse head is also wound around the fixing bolt 12; when facing different mice of different sizes, the distance between the two fixing bolts 12 in the threaded slot can be adjusted to fix mice of different sizes, thereby meeting the experimental requirements; at the same time, by increasing the number of turns of the fixing rope 13 wound on the fixing bolt 12, the length of the fixing rope 13 can be shortened, thereby achieving the effect of fixing and tightening the head of the mouse.

[0036] In this embodiment, the fixing sticker 3 is positioned near the temperature-regulating pad 2, and the fixing sticker 3 is symmetrically positioned at the four corners near the temperature-regulating pad 2, thereby fixing the four legs of the mouse and rat; specifically:

[0037] like Figure 5 , Figure 6As shown, the fixing patch 3 is an adjustable fixing patch, including an integrally formed fixing patch 31, a first adjusting fixing band 32, and a second adjusting fixing band 33. The two ends of the fixing patch 31 are respectively fixedly connected to the first adjusting fixing band 32 and the second adjusting fixing band 33. A groove 34 is provided on the first adjusting fixing band 32, and the first adjusting fixing band 32 passes through the groove 34 on the second adjusting fixing band to clamp and fix the limbs of the mouse placed on the fixing patch 31. For example, when fixing the limbs of a mouse or rat, the first adjusting fixing band 32 and the second adjusting fixing band 33 move in opposite directions closer to the fixing patch 31, so that one end of the first adjusting fixing band 32 passes through the groove 34 on the second adjusting fixing band 33, thereby wrapping around the limbs of the mouse or rat located on the fixing patch. At the same time, according to the size of different limbs of mice and rats, the size of the space formed by the first adjusting fixing band 32, the second adjusting fixing band 33, and the fixing patch 31 can be adjusted to match the limbs of different sizes of mice and rats, thereby completing the fixation.

[0038] Correspondingly, the fixation patch 31 is fixedly mounted on the mouse fixation plate 1; the first adjustment fixation strap 32 and the second adjustment fixation strap 33 are respectively attached to the mouse fixation plate 1 by means of hook and loop fasteners, specifically: both the first adjustment fixation strap 32 and the second adjustment fixation strap 33 are provided with hook and loop fasteners, and the mouse fixation plate 1 is provided with matching hook and loop fasteners, so that adjustable fixation can be achieved by means of hook and loop fasteners to meet experimental requirements; and this fixation method is not limited to hook and loop fasteners, but can also be other fixation methods, such as snap fasteners, etc.

[0039] In this embodiment, a fixing rod 4 is also fixedly installed on the mouse fixation plate 1; wherein, the fixing rod 4 has a spiral structure and a certain degree of toughness, and its structure is similar to a mobile phone holder, so that it can be bent in multiple directions to facilitate the adjustment of the optimal position and angle when injecting mice; correspondingly, a fixing clip 41 for fixing the infusion needle is also provided at the head end of the fixing rod 4, wherein the infusion needle 5 for infusing mice is provided in the fixing clip 41.

[0040] like Figure 4 As shown, the fixing clamp 41 includes a fixing sleeve 42 and a fixing component 43 disposed on the fixing sleeve. The fixing component includes bolts and nuts. By providing an opening 44 on the fixing sleeve 42, the injection needle 5 is located inside the fixing sleeve 42. Then, a threaded hole is provided at one end of the fixing sleeve near the opening, and the injection needle 5 inside the fixing sleeve is fixed and tightened by the fixing method of bolts and nuts.

[0041] In this embodiment, the perfusion needle 5 includes a perfusion needle 51 and a perfusion catheter 52. One end of the perfusion catheter 52 is connected to the perfusion needle 51, and the other end is connected to a three-way valve 53. The three-way valve 53 is used to connect to an external pumping system. Specifically, the perfusion catheter 52 can connect to two perfusion fluids according to different needs, such as one saline and one paraformaldehyde, and inject them into mice and rats through the perfusion needle 51. In addition, the volume and rate can be controlled by connecting to an external pumping system, thereby achieving the desired effect of quantitative and rate-controlled perfusion of the heart of mice and rats.

[0042] It should be noted that this utility model has a simple structure and is easy to operate. Its fixing sticker and multiple threaded slots can fix the limbs and head of mice of different sizes; its constant temperature pad can provide good body temperature maintenance for the mice; the infusion fixing rod can accurately fix the position of the infusion needle; and the externally connected infusion pump can quantitatively and at a constant speed to achieve the desired effect, thereby facilitating experimental operation, improving the success rate of experiments, expanding the research scope, and saving experimental costs.

[0043] The foregoing descriptions and embodiments are provided to enable those skilled in the art to understand and apply this invention. Those skilled in the art will readily make various modifications to these contents and apply the general principles described herein to other embodiments without inventive effort. Therefore, this invention is not limited to the foregoing descriptions and embodiments. Improvements and modifications made by those skilled in the art based on the disclosure of this invention without departing from its scope should be within the protection scope of this invention.

Claims

1. A fixed perfusion device for use in rodent experiments, characterized in that, include; A mouse restraint plate, wherein the mouse restraint plate is disposed on the operating table; A temperature-regulating pad is placed on the upper surface of the mouse restraint plate to maintain the mouse's body temperature; The fixing sticker is placed near the temperature-controlled pad to fix the mouse's hands and feet; Fixing rod; the fixing rod is mounted on the mouse fixation plate and is used to cooperate with the infusion needle; A perfusion needle, which is mounted on a fixed rod, is used to perfuse the heart of a mouse; The upper surface of the mouse fixation plate is provided with a threaded groove, and a fixing bolt is correspondingly provided in the threaded groove; a fixing rope for fixing the mouse's head is also wrapped around the fixing bolt.

2. The fixed perfusion device for use in size mouse experiments according to claim 1, characterized in that, The threaded slots are configured in multiple ways and are arranged at equal intervals in the horizontal direction.

3. The fixation and perfusion device for rat and mouse experiments according to claim 1, characterized in that, The fixing stickers are set to 4, and are symmetrically placed at the four corners near the constant temperature pad.

4. The fixation and perfusion device for rat and mouse experiments according to claim 1, characterized in that, The fixation patch is an adjustable fixation patch, comprising an integrally formed fixation patch, a first adjustment fixation band, and a second adjustment fixation band; the two ends of the fixation patch are respectively fixedly connected to the first adjustment fixation band and the second adjustment fixation band; and a groove is provided on the first adjustment fixation band, which passes through the groove on the second adjustment fixation band to clamp and fix the mouse's hands and feet placed on the fixation patch.

5. The fixation and perfusion device for rat and mouse experiments according to claim 4, characterized in that, The fixation patch is fixedly mounted on the mouse fixation plate; the first adjustment fixation strap and the second adjustment fixation strap are respectively attached to the mouse fixation plate by means of hook and loop fasteners.

6. The fixation and perfusion device for rat and mouse experiments according to claim 1, characterized in that, The fixing rod has a spiral structure and is flexible; the bottom end of the fixing rod is fixedly connected to the mouse fixing plate, and the head end is also provided with a fixing clip for fixing the infusion needle, and the infusion needle is provided in the fixing clip.

7. The fixation and perfusion device for rat and mouse experiments according to claim 6, characterized in that, The fixing clip includes a fixing sleeve and a fixing component disposed on the fixing sleeve.

8. The fixation and perfusion device for rat and mouse experiments according to claim 1, characterized in that, The infusion needle includes an infusion needle tip and an infusion conduit. One end of the infusion conduit is connected to the infusion needle tip, and the other end is connected to a three-way valve. The three-way valve is used to connect to an external pumping system.

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