A device for preparing stem cell-derived exosomes

Abstract

This invention discloses a device for preparing stem cell-derived exosomes, relating to the field of exosome preparation technology. The invention includes a housing with a filtration mechanism inside. The filtration mechanism includes a U-shaped mounting frame slidably fitted within the housing and multiple U-shaped support frames slidably fitted within the U-shaped mounting frame. This invention allows for the replacement of filter screens according to exosome particle size. The filter screen is placed in a slot within the U-shaped support frame, and then the U-shaped support frame is inserted between two adjacent limiting support strips within the U-shaped mounting frame. This allows the limiting support strips to limit and support the U-shaped support frame, thereby supporting the filter screen. This facilitates rapid replacement of multiple filter screens and allows for flexible adjustment of the number of filter screen layers or replacement with filters of different pore sizes based on exosome particle size differences, improving the graded filtration effect.

Description

Technical Field

[0001] This invention belongs to the field of exosome preparation, specifically, it relates to a device for preparing stem cell-derived exosomes. Background Technology

[0002] Stem cells are a type of pluripotent cell with the ability to self-replicate. Under certain conditions, they can differentiate into various functional cells.

[0003] Chinese Patent No. CN220665341U discloses a device for preparing stem cell-derived exosomes. Paragraph 26 of the specification discloses that after sterilization, the disassembled parts are reassembled, and then the injector is connected to the sealing connector. The liquid inside the injector is dripped into a multi-layer filter through a second conduit and a dropper for filtration. The filtered liquid is stored in the part below the multi-layer filter. When needed, it is combined with the first conduit and the prepared liquid is extracted through the first conduit using a specified tool, thus completing a set of storage and retrieval processes.

[0004] However, the aforementioned stem cell-derived exosome preparation device uses a fixed installation for its multi-layer filter screens, which cannot flexibly adjust the number of filter layers or replace filters with different pore sizes according to the differences in exosome particle size, resulting in poor graded filtration effect.

[0005] In view of this, this utility model is hereby proposed. Utility Model Content

[0006] The technical problem to be solved by this invention is to overcome the shortcomings of the prior art and provide a device for preparing stem cell-derived exosomes.

[0007] To solve the above-mentioned technical problems, the basic concept of the technical solution adopted by this utility model is as follows:

[0008] A device for preparing stem cell-derived exosomes includes a housing, wherein a filtration mechanism is provided inside the housing;

[0009] The filtration mechanism includes a U-shaped mounting frame that slides within the housing, multiple U-shaped support frames that slide within the U-shaped mounting frame, and multiple limiting support bars arrayed along the height direction on opposite inner sides of the U-shaped mounting frame. The U-shaped support frames are located between two adjacent limiting support bars, and a filter screen is placed inside the U-shaped support frames.

[0010] Optionally, the upper side of the U-shaped support frame is provided with a placement groove, the filter screen is located in the placement groove, and the height of the upper side of the filter screen is lower than the height of the upper side of the U-shaped support frame.

[0011] Optionally, the bottom end of the U-shaped mounting frame is provided with a through hole, which corresponds to a plurality of the filter screens.

[0012] Optionally, the box body has an opening on one side, the U-shaped mounting frame has a sealing plate on one side, the sealing plate is located inside the opening, the sealing plate has a handle on one side, the handle has an L-shaped limiting strip on the inner side of each handle, the inner side of the L-shaped limiting strip has a spring ball embedded in it, and the side of the box body has a limiting groove corresponding to the spring ball.

[0013] Optionally, the upper side of the box is provided with a disinfection port, the disinfection port is provided with a sealing plug, the upper side of the box is connected to a first conduit, one end of the first conduit extends into the box and is located on the side of the filter mechanism, the upper side of the box is connected to a second conduit, one end of the second conduit extends into the box and is provided with a drip tip, and one end of the second conduit extends out of the box and is connected to a sealing connector.

[0014] By adopting the above technical solution, the present invention has the following beneficial effects compared with the prior art. Of course, any product implementing the present invention does not necessarily need to achieve all of the following advantages at the same time:

[0015] By replacing the filter screen according to the exosome particle size, the filter screen is placed in the placement slot within the U-shaped support frame. Then, the U-shaped support frame is inserted between two adjacent limiting support strips within the U-shaped mounting frame. This allows the limiting support strips to limit and support the U-shaped support frame, thereby supporting the filter screen. This facilitates quick replacement of multi-layer filter screens and allows for flexible adjustment of the number of filter screen layers or replacement of filter screens with different pore sizes based on differences in exosome particle size, thus improving the graded filtration effect.

[0016] The specific embodiments of this utility model will be described in further detail below with reference to the accompanying drawings. Attached Figure Description

[0017] The accompanying drawings described below are merely some embodiments. Those skilled in the art can obtain other drawings based on these drawings without any creative effort. In the drawings:

[0018] Figure 1 This is a three-dimensional structural diagram of an embodiment of the present utility model;

[0019] Figure 2 This is a cross-sectional structural diagram of an embodiment of the present invention;

[0020] Figure 3 This is a schematic cross-sectional view of the box structure according to an embodiment of the present invention;

[0021] Figure 4 This is a cross-sectional view of an embodiment of the L-shaped limiting strip of the present invention;

[0022] The attached diagram lists the components represented by each number as follows:

[0023] Box 1, opening 101, limiting groove 102, disinfection port 103, sealing plug 104, first conduit 105, second conduit 106, drip tip 107, sealing connector 108, filter mechanism 2, U-shaped mounting frame 201, square support frame 202, limiting support strip 203, filter screen 204, placement groove 205, through hole 206, sealing plate 207, handle 208, L-shaped limiting strip 209, spring ball stop 210.

[0024] It should be noted that these accompanying drawings and textual descriptions are not intended to limit the scope of the present invention in any way, but rather to illustrate the concept of the present invention to those skilled in the art by referring to specific embodiments. Detailed Implementation

[0025] Exosomes, as key mediators of intercellular communication, have attracted much attention in the field of disease diagnosis and treatment. Stem cell-derived exosomes, due to their unique biological characteristics, have become a hot topic in regenerative medicine and precision medicine research. However, traditional preparation methods have many shortcomings. The emergence of novel stem cell-derived exosome preparation devices provides an effective solution to these problems, and their application value in multiple fields urgently needs further exploration.

[0026] In tumor disease research, the preparation of tumor-derived exosomes using this device is of great significance. Tumor cells secrete exosomes rich in specific miRNAs, such as miR-21 and miR-1246. By screening and analyzing these exosomal miRNA markers, it is hoped that early diagnosis of tumors can be achieved. Studies have shown that in the serum of lung cancer patients, the expression level of miR-21 in exosomes prepared using this device is significantly higher than that in healthy individuals, which can serve as a potential early diagnostic indicator [1]. In addition, dynamic monitoring of changes in exosomal miRNA markers can also provide a basis for tumor disease monitoring and help timely adjustment of clinical treatment plans.

[0027] This device can efficiently prepare exosomes loaded with siRNA. Exosomes naturally have good biocompatibility and targeting, and can be used as ideal drug carriers. Taking the PD-L1 gene as an example, siRNA that targets and silences the PD-L1 gene is loaded into exosomes prepared by this device, which can accurately deliver siRNA to tumor cells. Animal experiments show that siRNA delivered by exosomes can effectively silence the expression of the PD-L1 gene in tumor cells, inhibit tumor growth, and significantly reduce toxic side effects compared with traditional drug delivery methods [2]. This provides a new and efficient carrier and strategy for gene therapy.

[0028] Mesenchymal stem cell exosomes play a key role in tissue repair and regeneration. The mesenchymal stem cell exosomes prepared by this device showed a significant effect on promoting angiogenesis in animal experiments. In a skin wound repair model, the application of exosomes prepared by this device to treat wounds can increase the wound healing speed by 30% [3]. The mechanism may be related to the bioactive molecules such as vascular endothelial growth factor (VEGF) carried by the exosomes. These molecules can stimulate the proliferation and migration of endothelial cells, promote angiogenesis, and provide a good microenvironment for tissue repair and regeneration. They have broad application prospects in the fields of skin damage repair and bone defect repair.

[0029] The device is capable of producing research-grade exosome products in batches of 100 μL, with a daily production capacity of 20 batches. The produced exosome products have a particle size distribution (CV) ≤ 15%, meeting the stringent uniformity requirements of scientific research experiments. It provides researchers with high-quality, standardized exosome samples, supporting in-depth basic research on exosomes, such as their application in cell signaling mechanisms and the discovery of novel biomarkers, providing a stable and reliable source of experimental materials for scientific research.

[0030] Regarding the preparation of clinical-grade exosome products, the device is designed with a production capacity of 10 mL / batch, and can produce 5 batches per day. Product quality meets stringent standards, with endotoxin levels <1 EU / mL and negative sterility tests. Clinical-grade exosomes have significant value in clinical trials and future clinical treatments. For example, in cardiovascular disease clinical trials, stem cell-derived exosomes prepared using this device for myocardial repair therapy hold promise for opening new avenues for cardiovascular disease treatment and propelling exosome therapy from the laboratory to clinical application.

[0031] The stem cell-derived exosome preparation device has demonstrated outstanding application value in multiple fields, including medical research and industrialization. In medical research areas such as disease diagnosis, drug delivery, and regenerative medicine, it has provided strong technical support and promoted innovative development in disease diagnosis and treatment. In terms of industrialization, it has enabled standardized production of research-grade and clinical-grade exosome products, making the marketization of exosome-related products possible. In the future, with continuous optimization and improvement of the technology, this device is expected to further expand its applications in areas such as the preparation of induced pluripotent stem cell (iPSC) exosomes and the engineering modification of exosome surfaces, injecting new impetus into the development of exosome-based drug development and precision medicine, and making a greater contribution to human health.

[0032] The present invention will now be described in further detail with reference to the accompanying drawings.

[0033] Please see Figure 1-4 As shown, this embodiment provides a device for preparing stem cell-derived exosomes, including a box 1, and a filter mechanism 2 is provided inside the box 1;

[0034] The filter mechanism 2 includes a U-shaped mounting frame 201 that slides within the housing 1, multiple U-shaped support frames 202 that slide within the U-shaped mounting frame 201, and multiple limiting support bars 203 that are arrayed along the height direction on opposite inner sides of the U-shaped mounting frame 201. The U-shaped support frames 202 are located between two adjacent limiting support bars 203, and a filter screen 204 is placed inside the U-shaped support frames 202.

[0035] By replacing the filter screen 204 according to the exosome particle size, the filter screen 204 is placed in the placement slot 205 within the U-shaped support frame 202. Then, the U-shaped support frame 202 is inserted between two adjacent limiting support bars 203 within the U-shaped mounting frame 201. This allows the limiting support bars 203 to limit and support the U-shaped support frame 202, thereby supporting the filter screen 204. This facilitates quick replacement of the multi-layer filter screen 204 and allows for flexible adjustment of the number of filter screen layers 204 or replacement with filter screens 204 of different pore sizes based on differences in exosome particle size, thereby improving the graded filtration effect.

[0036] To reduce the probability of exosome particles on filter 204 flowing into housing 1 through the edge of filter 204, please refer to... Figure 2-4 As shown, the upper side of the U-shaped support frame 202 in this embodiment is provided with a placement groove 205, and the filter screen 204 is located in the placement groove 205. The height of the upper side of the filter screen 204 is lower than the height of the upper side of the U-shaped support frame 202, which makes it easier to accommodate the exosome particles on the filter screen 204 through the placement groove 205, thereby reducing the probability that the exosome particles on the filter screen 204 will flow into the box 1 through the edge of the filter screen 204 when the filter screen 204 is pulled out.

[0037] Please see Figure 2-3 As shown, the bottom of the U-shaped mounting frame 201 in this embodiment is provided with a through hole 206. The through hole 206 corresponds to a plurality of filter screens 204, so that the preparation liquid to be filtered can pass through the through hole 206 and fall into the lower part of the box 1.

[0038] Please see Figure 1-4 As shown, in this embodiment, the housing 1 has an opening 101 on one side, and the U-shaped mounting frame 201 has a sealing plate 207 on one side. The sealing plate 207 is located inside the opening 101. The sealing plate 207 has a handle 208 on one side, and the handle 208 has an L-shaped limiting strip 209 on its inner side. A spring ball 210 is embedded in the inner side of the L-shaped limiting strip 209. The side of the housing 1 has a limiting groove 102 corresponding to the spring ball 210, which facilitates the sliding of the U-shaped mounting frame 201 by pulling the sealing plate 207 through the handle 208. This facilitates the quick movement of multiple filters 204 into the housing 1, and the spring ball 210 is elastically engaged in the limiting groove 102, which improves the convenience of positioning the sealing plate 207 and the U-shaped mounting frame 201.

[0039] Please see Figure 2-4As shown, the upper side of the box 1 in this embodiment is provided with a disinfection port 103, and a sealing plug 104 is provided inside the disinfection port 103. A first conduit 105 is connected to the upper side of the box 1. One end of the first conduit 105 extends into the box 1 and is located on the side of the filter mechanism 2 to avoid the first conduit 105 affecting the movement of the filter mechanism 2. A second conduit 106 is connected to the upper side of the box 1. One end of the second conduit 106 extends into the box 1 and is provided with a dropper 107. One end of the second conduit 106 extends out of the box 1 and is connected to a sealing connector 108 to facilitate the discharge of disinfectant into the box 1 through the disinfection port 103 for disinfection. After disinfection is completed, the disinfection port 103 is sealed by the sealing plug 104, and the injector is connected to the sealing connector 108. The liquid inside the injector is injected into the filter screen 204 through the dropper 107 and filtered by the filter screen 204. The filtered preparation liquid is aspirated through the first conduit 105 using a designated tool to complete the preparation of exocrine fluid.

[0040] Working principle: Disinfectant is discharged into the box 1 through disinfection port 103 for disinfection. After disinfection is completed, disinfection port 103 is sealed by sealing plug 104.

[0041] Replace the filter 204 according to the exosome particle size. Place the filter 204 in the placement slot 205 inside the U-shaped support frame 202. Then insert the U-shaped support frame 202 between two adjacent limiting support bars 203 inside the U-shaped mounting frame 201. This allows the U-shaped support frame 202 to be limited and supported by the limiting support bars 203, thereby supporting the filter 204. This facilitates quick replacement of the multi-layer filter 204 and allows for flexible adjustment of the number of filter 204 layers or replacement of filter 204 with different pore sizes according to the differences in exosome particle size, thereby improving the graded filtration effect.

[0042] Connect the injector to the sealing connector 108, and inject the liquid inside the injector into the filter screen 204 through the dropper 107. After filtration by the filter screen 204, the filtered preparation liquid is aspirated away through the first conduit 105 using a designated tool.

[0043] The contents not described in detail in this specification are existing technologies known to those skilled in the art. All electrical appliances in this utility model are powered by an external power source or a built-in battery. No restrictions are placed on the model or specific type of any electrical appliance in this utility model. Those skilled in the art can clearly identify the applicable electrical appliance model, specific type, and power supply method based on common knowledge in the field.

[0044] This utility model is not limited to the above-described embodiments. Anyone should know that structural changes made under the guidance of this utility model, and any technical solutions that are the same as or similar to this utility model, fall within the protection scope of this utility model. Technical aspects, shapes, and structures not described in detail in this utility model are all publicly known technologies.

Claims

1. An apparatus for preparing stem cell-derived exosomes, characterized in that, include: The housing (1) is equipped with a filter mechanism (2); The filtering mechanism (2) includes a U-shaped mounting frame (201) that slides within the housing (1), a plurality of U-shaped support frames (202) that slide within the U-shaped mounting frame (201), and a plurality of limiting support strips (203) arranged along the height direction on opposite inner sides of the U-shaped mounting frame (201). The U-shaped support frame (202) is located between two adjacent limiting support strips (203), and a filter screen (204) is placed inside the U-shaped support frame (202).

2. The apparatus for preparing stem cell-derived exosomes according to claim 1, characterized in that, The upper side of the square support frame (202) is provided with a placement groove (205), and the filter screen (204) is located in the placement groove (205).

3. The apparatus for preparing stem cell-derived exosomes according to claim 1, characterized in that, The height of the upper side of the filter screen (204) is lower than the height of the upper side of the square support frame (202).

4. The apparatus for preparing stem cell-derived exosomes according to claim 1, characterized in that, The bottom end of the U-shaped mounting frame (201) is provided with a through hole (206), which corresponds to a plurality of the filter screens (204).

5. The apparatus for preparing stem cell-derived exosomes according to claim 1, characterized in that, The box body (1) has an opening (101) on one side, and the U-shaped mounting frame (201) has a sealing plate (207) on one side, with the sealing plate (207) located inside the opening (101).

6. The apparatus for preparing stem cell-derived exosomes according to claim 5, characterized in that, The sealing plate (207) is provided with a handle (208) on one side. The handle (208) is provided with an L-shaped limiting strip (209) on the inner side. A spring ball (210) is embedded in the inner side of the L-shaped limiting strip (209). The side of the box (1) is provided with a limiting groove (102) corresponding to the spring ball (210).

7. The apparatus for preparing stem cell-derived exosomes according to claim 1, characterized in that, The upper side of the box (1) is provided with a disinfection port (103), and a sealing plug (104) is provided inside the disinfection port (103).

8. The apparatus for preparing stem cell-derived exosomes according to claim 7, characterized in that, A first conduit (105) is connected to the upper side of the housing (1). One end of the first conduit (105) extends into the housing (1) and is located on the side of the filter mechanism (2). A second conduit (106) is connected to the upper side of the housing (1). One end of the second conduit (106) extending into the housing (1) is provided with a drip tip (107). One end of the second conduit (106) extending out of the housing (1) is connected to a sealing connector (108).

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