A covalent inhibitor reaction screening device

Abstract

This utility model relates to the field of screening equipment technology, and more particularly to a screening device for covalent inhibitor reactions. It includes a screening device body, on the inner wall of which a microwell plate is installed. Four foot pads are installed on one side of the screening device body. An adjustment mechanism is provided at one end of the screening device body. The adjustment mechanism includes two fixing plates, each fixedly connected to one end of the screening device body. A motor is fixedly connected to one side of each fixing plate, and a gear is fixedly connected to the output end of the motor. The gear teeth mesh with a rack. This utility model provides a covalent inhibitor reaction screening device that allows the microwell plate to be placed in the screening device by means of the adjustment mechanism, replacing manual labor. The adjustment mechanism improves the stability of microwell plate placement, reduces manpower consumption, increases laboratory efficiency, and enhances experimental accuracy.

Description

Technical Field

[0001] This utility model relates to the field of screening equipment technology, and in particular to a covalent inhibitor reaction screening device. Background Technology

[0002] Screening equipment is a device used to efficiently screen small molecule compounds that covalently bind to target proteins. This device is widely used in drug discovery and biochemical research, especially in the search for small molecule compounds that can bind to proteins through covalent bonds and inhibit their function.

[0003] Utility model patent CN213644883U discloses a screening device for TTR small molecule inhibitors. The key technical features are: a screening box containing a fixed plate; a screening groove within the screening box; a filtering mechanism within the screening groove; a motor on the fixed plate; a power mechanism on the motor's output shaft to provide power for screening; an opening at the top of the screening box; a feed pipe slidably connected within the opening; the feed pipe extending through the opening into the screening groove; a mounting plate fixedly connected to the left end of the screening box; a rotating shaft rotatably connected to the mounting plate; a reciprocating mechanism on the mounting plate to move the feed pipe back and forth; and a transmission mechanism on the motor's output shaft to provide power for the reciprocating mechanism. This utility model has a reasonable structure, enabling the feed pipe to move back and forth, preventing material accumulation. It is also simple in structure and suitable for small-scale screening.

[0004] Regarding the aforementioned content, the following technical defects exist: Screening devices are used in drug discovery and biochemical research. A test sample is added to a microplate, and the microplate is pushed into the screening device. The device uses a liquid dispenser to add covalent inhibitors one by one into the test ports of the microplate, thereby screening out small molecule compounds that can bind to proteins through covalent bonds and inhibit their function. However, the manual placement of the microplate requires individual operation, and each movement of the microplate takes time. This is very time-consuming when conducting a large number of experiments, especially in high-throughput screening, where manual operation becomes a bottleneck, limiting the experimental throughput and overall efficiency. Manual operation is also affected by the operator's skill level and energy level. External factors can lead to inaccurate placement, positional deviations, or incomplete alignment of the microplate, thus affecting the reliability and consistency of the experimental results.

[0005] Therefore, it is necessary to provide a new covalent inhibitor reaction screening device to solve the above-mentioned technical problems. Utility Model Content

[0006] The purpose of this invention is to solve the problem in the existing technology that involves manually placing microplates, which requires personnel to operate one by one. Each movement of the microplate takes time, which is very time-consuming when a large number of experiments are needed. Especially in high-throughput screening, manual operation becomes a bottleneck, limiting the throughput and overall efficiency of the experiment. Manual operation is also affected by the operator's skill level and energy level. Under the influence of external factors, it may lead to inaccurate placement of microplates, positional deviation, or incomplete alignment, thereby affecting the reliability and consistency of experimental results.

[0007] To solve the above-mentioned technical problems, this utility model provides a covalent inhibitor reaction screening device, comprising: a screening device body, a microporous plate installed on the inner wall of the screening device body, four foot pads installed on one side of the screening device body, an adjustment mechanism provided at one end of the screening device body, the adjustment mechanism including two fixing plates, both of which are fixedly connected to one end of the screening device body, a motor fixedly connected to one side of the fixing plates, a gear fixedly connected to the output end of the motor, a rack meshing with the tooth surface of the gear, a connecting groove opened on the side of the rack, a guide block slidably connected to the inner wall of the connecting groove, one end of the arc surface of the guide block being fixedly connected to one side of the inner wall of the screening device body, a common moving stage fixedly connected to one end of the two racks, and an electric push rod fixedly connected to the bottom wall of the screening device body, the output end of the electric push rod being slidably connected to the inner wall of the moving stage.

[0008] The effect achieved by the aforementioned components is as follows: The screening device body is used in drug discovery and biochemical research. Test samples are added to microplates, and the microplates are pushed into the screening device body. The screening device body, through a liquid dispenser, can add covalent inhibitors one by one into the test ports of the microplate, thereby screening out small molecule compounds that can bind to proteins through covalent bonds and inhibit their function. However, the manual insertion of microplates requires personnel to operate one by one, and each movement of the microplate takes time. This is very time-consuming when conducting a large number of experiments, especially in high-throughput screening, where manual operation becomes extremely costly. The bottleneck restricts the throughput and overall efficiency of experiments. Manual operation is also affected by the operator's skill level and energy level. External factors can lead to inaccurate placement of microplates, positional deviations, or incomplete alignment, thus affecting the reliability and consistency of experimental results. In this case, an adjustment mechanism can be used to replace manual placement of microplates. The adjustment mechanism can place the microplates into the screening device body, which can significantly improve efficiency and accuracy, reduce manual intervention, and achieve fully automated operation. This saves time, improves laboratory work efficiency, reduces errors caused by human operation, and ensures high precision of experiments.

[0009] Preferably, the output end of the electric actuator is fixedly connected to a connecting block, and the cross-section of the connecting block is arc-shaped.

[0010] The effect achieved by the above components is that the connecting block can prevent damage when the output end of the electric actuator comes into contact with the inner wall of the moving platform.

[0011] Preferably, a friction pad is fixedly connected to the side of the moving platform away from the rack, and the friction pad has anti-slip texture on the side away from the moving platform.

[0012] The effect achieved by the above components is that the friction pad can improve the contact effect between the microporous plate and the moving stage.

[0013] Preferably, a limiting disk is fixedly connected to the side of the gear away from the motor.

[0014] The effect achieved by the above components is that the limiting disc can improve the stability of the meshing between the gear and the rack.

[0015] Preferably, each of the four foot pads has an auxiliary mechanism on the side near the main body of the screening device. The auxiliary mechanism includes a limiting frame, which is fixedly connected to one side of the main body of the screening device. Assembly blocks are fixedly connected to both sides of the limiting frame. An installation block is fixedly connected to the top wall of the main body of the screening device. A connecting hole is opened on the foot pad corresponding to the position of the installation block. The installation block is slidably connected to the inner wall of the connecting hole. A fixing block is fixedly connected to one end of the arc surface of the foot pad. The same limiting rod is threaded through the inner wall of the two assembly blocks. The limiting rod is threaded through the inner wall of the fixing block.

[0016] The effect achieved by the above components is that when the foot pads on the main body of the screening device become deformed or damaged after long-term use, the deformed or damaged foot pads can be replaced by the auxiliary mechanism. By replacing the foot pads with new ones, the stability of the foot pads in subsequent use can be improved, and the use effect can be enhanced.

[0017] Preferably, a plurality of anti-slip grooves are provided at one end of the arc surface of the limiting rod, and the plurality of anti-slip grooves are evenly distributed on the limiting rod.

[0018] The effect achieved by the above components is that the anti-slip groove can increase the speed at which the limit rod rotates within the two assembly blocks.

[0019] Preferably, a connecting block is fixedly connected to the side of the mounting block near the connecting hole, and the cross-section of the connecting block is arc-shaped.

[0020] The effect achieved by the above components is that the connecting block enables the mounting block to connect to the inner wall of the connecting hole more quickly.

[0021] Compared with related technologies, the covalent inhibitor reaction screening device provided by this utility model has the following beneficial effects:

[0022] By setting up an adjustment mechanism, the microplate can be placed in the screening device instead of manually. This mechanism improves the stability of microplate placement, reduces manpower consumption, increases laboratory efficiency, and enhances experimental accuracy.

[0023] By setting up an auxiliary mechanism, when the foot pads become deformed or damaged after long-term use, they can be replaced through the auxiliary mechanism. Replacing the foot pads through the auxiliary mechanism can improve the stability of the foot pads in supporting the screening device and improve the balance effect of the screening device during use. Attached Figure Description

[0024] Figure 1 A schematic diagram of the structure of a covalent inhibitor reaction screening device provided by this utility model;

[0025] Figure 2 for Figure 1 The diagram shows the structure of the adjustment mechanism;

[0026] Figure 3 for Figure 1 A schematic diagram of the enlarged structure of the adjustment mechanism shown;

[0027] Figure 4 for Figure 1 The diagram shows the structure of the auxiliary mechanism.

[0028] Figure 5 for Figure 1 The diagram shows the disassembled structure of the auxiliary mechanism.

[0029] The following are the labeling elements in the diagram: 1. Screening device body; 2. Adjustment mechanism; 201. Fixing plate; 202. Motor; 203. Gear; 204. Rack; 205. Moving stage; 206. Electric push rod; 207. Guide block; 208. Connecting groove; 209. Connecting block; 210. Friction pad; 211. Limiting plate; 3. Auxiliary mechanism; 31. Connecting hole; 32. Mounting block; 33. Limiting frame; 34. Fixing block; 35. Assembly block; 36. Limiting rod; 37. Anti-slip groove; 38. Connecting block; 4. Microporous plate; 5. Foot pad. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of this utility model clearer, the present utility model will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the present utility model and are not intended to limit the present utility model.

[0031] The specific implementation of this utility model will be described in detail below with reference to specific embodiments.

[0032] Please see Figures 1 to 5 The present invention provides a covalent inhibitor reaction screening device, comprising: a screening device body 1, a microporous plate 4 installed on the inner wall of the screening device body 1, four foot pads 5 installed on one side of the screening device body 1, an adjustment mechanism 2 provided at one end of the screening device body 1, and an auxiliary mechanism 3 provided on the side of each of the four foot pads 5 near the screening device body 1.

[0033] In the embodiments of this utility model, please refer to Figure 2 and Figure 3The adjusting mechanism 2 includes two fixed plates 201, both of which are fixedly connected to one end of the screening device body 1. A motor 202 is fixedly connected to one side of the fixed plate 201. A gear 203 is fixedly connected to the output end of the motor 202. A rack 204 meshes with the tooth surface of the gear 203. A connecting groove 208 is provided on the side of the rack 204. A guide block 207 is slidably connected to the inner wall of the connecting groove 208. One end of the arc surface of the guide block 207 is fixedly connected to one side of the inner wall of the screening device body 1. The same moving platform 205 is fixedly connected to one end of the two racks 204. An electric push rod 206 is fixedly connected to the bottom wall of the screening device body 1. The output end of the electric push rod 206 is slidably connected to the inner wall of the moving platform 205. The screening device body 1 is used in drug discovery and biochemical research. Test samples are added to microplates 4, and the microplates 4 are pushed into the screening device body 1. The screening device body 1 uses a liquid dispenser to add covalent inhibitors one by one into the test ports of the microplates 4, thereby screening out small molecule compounds that can bind to proteins through covalent bonds and inhibit their function. However, manually placing the microplates 4 requires individual operation, and each movement of the microplates 4 takes time. This is very time-consuming when conducting a large number of experiments, especially in high-throughput screening, where manual operation becomes a bottleneck, limiting the throughput and overall efficiency of the experiments. Manual operation is also affected by the operator's skill level and energy level. External factors can lead to inaccurate placement, positional deviation, or misalignment of the microplates 4, thus affecting the reliability and consistency of the experimental results. In such cases, the adjustment mechanism 2 can replace manual operation. The microplate 4 is manually placed into the screening device body 1. The adjustment mechanism 2 can significantly improve efficiency and accuracy, reduce manual intervention, and achieve fully automated operation, saving time and improving laboratory work efficiency. It also reduces errors caused by human operation and ensures high precision of the experiment. The output end of the electric push rod 206 is fixedly connected to the connecting block 209. The cross-section of the connecting block 209 is arc-shaped. The connecting block 209 can prevent damage when the output end of the electric push rod 206 contacts the inner wall of the moving stage 205. The side of the moving stage 205 away from the rack 204 is fixedly connected to the friction pad 210. The side of the friction pad 210 away from the moving stage 205 has anti-slip texture. The friction pad 210 can improve the contact effect between the microplate 4 and the moving stage 205. The side of the gear 203 away from the motor 202 is fixedly connected to the limiting plate 211. The limiting plate 211 can improve the stability of the meshing between the gear 203 and the rack 204.

[0034] In the embodiments of this utility model, please refer to Figure 4 and Figure 5The auxiliary mechanism 3 includes a limiting frame 33, which is fixedly connected to one side of the screening device body 1. Assembly blocks 35 are fixedly connected to both sides of the limiting frame 33. An installation block 32 is fixedly connected to the top wall of the screening device body 1. A connecting hole 31 is opened on the foot pad 5 corresponding to the position of the installation block 32. The installation block 32 is slidably connected to the inner wall of the connecting hole 31. A fixing block 34 is fixedly connected to one end of the arc surface of the foot pad 5. The same limiting rod 36 is threaded through the inner wall of the two assembly blocks 35. The limiting rod 36 is threaded through the inner wall of the fixing block 34. The foot pad 5 on the screening device body 1 deforms after long-term use. If damaged, the deformed or damaged foot pad 5 can be replaced by the auxiliary mechanism 3. By replacing the foot pad 5 with a new one, the subsequent stability of the foot pad 5 can be improved and the performance can be enhanced. Several anti-slip grooves 37 are provided at one end of the arc surface of the limiting rod 36. The anti-slip grooves 37 are evenly distributed on the limiting rod 36. The anti-slip grooves 37 can increase the rotation speed of the limiting rod 36 within the two assembly blocks 35. A connecting block 38 is fixedly connected to the side of the mounting block 32 near the connecting hole 31. The cross section of the connecting block 38 is arc-shaped. The connecting block 38 enables the mounting block 32 to connect with the inner wall of the connecting hole 31 more quickly.

[0035] The working principle of the covalent inhibitor reaction screening device provided by this utility model is as follows: When it is necessary to place the microporous plate 4 into the screening device body 1, the screening device body 1 is placed on the moving stage 205. At this time, the motor 202 installed on the fixed plate 201 can be started. The movement of the motor 202 will drive the gear 203 to rotate through the output end. The rotation of the gear 203 will drive the rack 204 to move. The rack 204 will slide on the guide rod through the connecting groove 208. At this time, the rack 204 can move into the screening device body 1. The movement of the rack 204 will drive the moving stage 205 to move. The movement of the moving stage 205 can drive the microporous plate 4 into the screening device body 1. The electric push rod 206 is started. The output end of the electric push rod 206 will connect with the inner wall of the moving stage 205. At this time, the position of the moving stage 205 can be fixed.

[0036] Rotate the limiting rod 36 within the assembly block 35 to move the limiting rod 36 out of the assembly block 35 and the fixed block. At this time, the foot pad 5 and the fixed block 34 can be removed from the screening device body 1 within the mounting block 32 and the limiting frame 33. Then, connect the connecting hole 31 on the new foot pad 5 to the arc surface of the mounting block 32. At this time, the foot pad 5 will be in the limiting frame 33. Make the fixed block 34 contact the middle position of the two assembly blocks 35. Then, rotate the limiting rod 36 within the assembly block 35 and the fixed block 34 to fix the new foot pad 5 to one side of the screening device body 1.

[0037] The circuits and controls involved in this utility model are all existing technologies, and will not be described in detail here.

[0038] The above description is merely an embodiment of this utility model and does not limit the patent scope of this utility model. Any equivalent structural or procedural transformations made based on the content of this utility model specification and drawings, or direct or indirect applications in other related technical fields, are similarly included within the patent protection scope of this utility model.

Claims

1. A covalent inhibitor reaction screening device, characterized in that, include: The screening device body (1) has a microporous plate (4) installed on its inner wall. Four foot pads (5) are installed on one side of the screening device body (1). An adjustment mechanism (2) is provided at one end of the screening device body (1). The adjustment mechanism (2) includes two fixing plates (201). Both fixing plates (201) are fixedly connected to one end of the screening device body (1). A motor (202) is fixedly connected to one side of the fixing plate (201). A gear (203) is fixedly connected to the output end of the motor (202). The toothed surfaces of (203) are engaged with a rack (204). A connecting groove (208) is provided on the side of the rack (204). A guide block (207) is slidably connected to the inner wall of the connecting groove (208). One end of the arc surface of the guide block (207) is fixedly connected to one side of the inner wall of the screening device body (1). One end of the two racks (204) is fixedly connected to the same moving platform (205). An electric push rod (206) is fixedly connected to the bottom wall of the screening device body (1). The output end of the electric push rod (206) is slidably connected to the inner wall of the moving platform (205).

2. The covalent inhibitor reaction screening device according to claim 1, characterized in that, The output end of the electric actuator (206) is fixedly connected to a connecting block (209), and the cross-section of the connecting block (209) is arc-shaped.

3. The covalent inhibitor reaction screening device according to claim 1, characterized in that, A friction pad (210) is fixedly connected to the side of the moving platform (205) away from the rack (204), and the side of the friction pad (210) away from the moving platform (205) has anti-slip texture.

4. The covalent inhibitor reaction screening device according to claim 1, characterized in that, The gear (203) is fixedly connected to a limiting disk (211) on the side away from the motor (202).

5. The covalent inhibitor reaction screening device according to claim 1, characterized in that, Each of the four foot pads (5) is provided with an auxiliary mechanism (3) on one side near the screening device body (1). The auxiliary mechanism (3) includes a limiting frame (33), which is fixedly connected to one side of the screening device body (1). Assembly blocks (35) are fixedly connected to both sides of the limiting frame (33). An installation block (32) is fixedly connected to the top wall of the screening device body (1). A connecting hole (31) is opened on the foot pad (5) corresponding to the position of the installation block (32). The installation block (32) is slidably connected to the inner wall of the connecting hole (31). A fixing block (34) is fixedly connected to one end of the arc surface of the foot pad (5). The same limiting rod (36) is threaded through the inner wall of the two assembly blocks (35). The limiting rod (36) is threaded through the inner wall of the fixing block (34).

6. The covalent inhibitor reaction screening device according to claim 5, characterized in that, The limiting rod (36) has several anti-slip grooves (37) at one end of its arc surface, and the several anti-slip grooves (37) are evenly distributed on the limiting rod (36).

7. The covalent inhibitor reaction screening device according to claim 5, characterized in that, The mounting block (32) is fixedly connected to a connecting block (38) on the side near the connecting hole (31), and the cross section of the connecting block (38) is arc-shaped.

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