Continuous flow centrifugation separation cup and cell separation device

Abstract

The utility model discloses a continuous flow centrifugal separation cup, which comprises an elbow joint, an upper cup body, a lower cup body and a rotary connecting piece, the upper cup body and the lower cup body are oppositely arranged and sealingly connected to form a centrifugal chamber, one end of the upper cup body away from the lower cup body is provided with a connecting pipe, the lower end of the elbow joint extends into the centrifugal chamber through the connecting pipe, the rotary connecting piece comprises a bearing and a bearing sleeve, the bearing is installed on the connecting pipe of the upper cup body, the bearing sleeve is installed on the elbow joint, the bearing sleeve cooperates with the bearing to realize the rotary connection between the upper cup body and the elbow joint, the utility model aims to overcome the defects of the prior art and provide a continuous flow centrifugal separation cup and a cell separation device, improve the yield of cell products, and avoid the pollution of cell products by insoluble particles generated by friction.

Description

Technical Field

[0001] This utility model relates to the field of cell separation technology, specifically a continuous flow centrifugal separation cup and a cell separation device. Background Technology

[0002] In recent years, the domestic biopharmaceutical and cell therapy industries have developed rapidly. In the later stages of biopharmaceutical and cell therapy, centrifugal separation equipment is usually used to concentrate and wash cell products to achieve a certain density before they are used in later stages.

[0003] Currently, most centrifuges on the market use a rotary seal structure between the stationary and rotating parts for sealing and auxiliary flow control. During high-speed rotation, the friction of the sealing ring within the rotary seal structure generates insoluble particles. These particles can mix with the cell product during concentration. Excessive levels of insoluble particles can contaminate the cell product, rendering it unusable and directly causing batch failures in cell drug production, or requiring additional processing steps to remove the particles. Furthermore, wear and tear on the rotary seal can cause it to lose its auxiliary flow control function, allowing the cell concentrate to easily flow directly from the center of the cup (where the rotary seal structure is located), resulting in low cell product yield.

[0004] Therefore, a continuous flow centrifugal separation cup is proposed to address the above problems. Utility Model Content

[0005] The purpose of this invention is to overcome the existing defects by providing a continuous flow centrifugal separation cup and cell separation device to improve the yield of cell products.

[0006] To achieve the above objectives, this utility model provides the following technical solution: a continuous flow centrifugal separation cup, comprising: a bent pipe connector, an upper cup body, a lower cup body, and a rotating connecting component; The upper cup body and the lower cup body are arranged opposite to each other and sealed to form a centrifuge chamber. The upper cup body has a connecting tube at the end facing away from the lower cup body. The lower end of the bent tube joint extends into the centrifuge chamber through the connecting tube. The rotating connector includes a bearing and a bearing sleeve. The bearing is installed on the connecting pipe of the upper cup body, and the bearing sleeve is installed on the bent pipe joint. The bearing sleeve cooperates with the bearing to realize the rotating connection between the upper cup body and the bent pipe joint. The top surface of the elbow joint is provided with a liquid outlet, and the side surface of the elbow joint is provided with a liquid inlet. The elbow joint has an inlet flow channel and an outlet flow channel. One end of the inlet flow channel is connected to the inlet, and the other end of the inlet flow channel is connected to the top of the centrifuge chamber. One end of the outlet flow channel is connected to the outlet, and the other end of the outlet flow channel is connected to the bottom of the centrifuge chamber. The upper cup body is fitted with a rotating sealing ring on the connecting tube. The rotating sealing ring is located between the outer wall of the connecting tube and the inner wall of the bearing sleeve, and is used to achieve a rotating seal between the upper cup body and the bearing sleeve.

[0007] The inlet channel of the elbow joint includes a horizontal section and a vertical section that are interconnected. The front end of the horizontal section is connected to the inlet, and the rear end of the horizontal section is connected to the upper end of the vertical section. The cross-section of the vertical section is an annular structure, and the annular structure surrounds the outlet channel. The outlet channel of the elbow joint is vertically arranged, and the upper end of the outlet channel is connected to the outlet. The lower end of the outlet channel extends out of the inlet channel.

[0008] It also includes: a primary fixed guide fluid and a secondary fixed guide fluid; both the primary fixed guide fluid and the secondary fixed guide fluid have a central opening and are sleeved on the elbow joint, and both the primary fixed guide fluid and the secondary fixed guide fluid are interference-fitted to the outer wall of the elbow joint; Both the primary fixed guide fluid and the secondary fixed guide fluid are located in the centrifuge chamber, and the primary fixed guide fluid is located between the secondary fixed guide fluid and the bearing sleeve.

[0009] It also includes: a primary rotating guide fluid and a secondary rotating guide fluid; both the primary rotating guide fluid and the secondary rotating guide fluid have openings in the middle, and the lower end of the bent pipe joint passes through the central holes of the primary rotating guide fluid and the secondary rotating guide fluid; Both the primary and secondary rotating guide fluids are located within the centrifuge chamber. The primary rotating guide fluid is fixedly connected to the inner wall of the upper cup, and the edge of the secondary rotating guide fluid is connected to the edge of the primary rotating guide fluid. The secondary fixed guide fluid is located between the primary rotating guide fluid and the secondary rotating guide fluid.

[0010] The upper surface edge of the primary rotating guide fluid is provided with multiple protrusions, which are equidistantly arranged along the circumferential direction. The inner wall of the upper cup body is provided with multiple recesses, which are corresponding to and fixedly connected to the multiple protrusions. The edge of the secondary rotating guide fluid is provided with an annular inner edge, which has several locking platforms, and the edge of the primary rotating guide fluid is engaged with the edge of the secondary rotating guide fluid.

[0011] The upper cup body has a boss structure, the lower end of the bearing abuts against the boss structure, the boss structure is hollow inside and communicates with the connecting pipe; the primary fixed guide fluid is located in the boss structure, the upper surface of the primary rotating guide fluid has an annular protrusion structure, the annular protrusion structure is inserted into the annular groove of the primary fixed guide fluid, and there is a gap between the annular protrusion structure and the groove wall of the annular groove.

[0012] A cup body sealing ring is provided between the lower cup body and the upper cup body; an upper clamp is provided on the edge of the upper cup body, and a lower clamp is provided on the edge of the lower cup body; the upper clamp and the lower clamp are connected by bolts. The bend joint is connected to the bearing sleeve via a flange, and a first sealing ring is provided between the flange and the bearing sleeve.

[0013] The end of the connecting tube is provided with a limiting ring. The inner ring of the limiting ring has a stepped hole and is interference-fitted onto the connecting tube of the upper cup body. The lower end presses against the rotating sealing ring.

[0014] It also includes a dust guard ring, which is disposed on the inner wall of the bearing sleeve and located between the limiting ring and the rotary sealing ring.

[0015] A cell separation device includes: a continuous flow centrifuge cup as described above.

[0016] Compared with the prior art, the beneficial effects of this utility model are as follows: This continuous flow centrifugal separation cup has a rotary sealing structure set on the outside of the cup body, and the rotary sealing structure is isolated from the cell product concentrate by using air isolation. This prevents insoluble particles generated by the wear of the sealing ring of the rotary sealing structure from contacting the cell product concentrate, thereby avoiding contamination of the cell product by insoluble particles generated by the wear of the sealing ring of the rotary sealing structure.

[0017] The flow of cell stock solution is guided by a multi-stage alternating arrangement of fixed and rotating guide fluids. The fixed and rotating guide fluids do not come into contact, which effectively avoids the generation of insoluble particles due to contact friction while completing the flow guidance. At the same time, the multi-stage flow guidance process allows the cell stock solution to flow along the required path, thereby improving the yield of cell products. Attached Figure Description

[0018] The accompanying drawings are provided to further illustrate the present invention and form part of the specification. They are used together with the embodiments of the present invention to explain the present invention, but do not constitute a limitation thereof. In the drawings: Figure 1 This is a cross-sectional view of the fixed part in the continuous flow centrifugal separation cup of this utility model; Figure 2 This is a cross-sectional view of the rotating part in the continuous flow centrifugal separation cup of this utility model; Figure 3 This is a cross-sectional view of the continuous flow centrifugal separation cup of this utility model; Figure 4 This is a schematic diagram showing the liquid flow direction during the operation of the cell separation device of this utility model; Figure 5 This is a schematic diagram illustrating the insoluble particle isolation of this utility model; Figure 6 This is a three-dimensional partial sectional view of the pipe bend joint of this utility model; Figure 7 This is a three-dimensional partial sectional view of the primary fixed fluid guide of this utility model; Figure 8 This is a three-dimensional view of the primary rotating guide fluid of this utility model; Figure 9 This is a three-dimensional partial cross-sectional view of the secondary fixed fluid guide of this utility model; Figure 10 This is a three-dimensional view of the secondary rotating guide fluid of this utility model; Figure 11 This is a three-dimensional cross-sectional view of the continuous flow centrifugal separation cup of this utility model.

[0019] In the diagram: 1. Bend joint; 2. First sealing ring; 3. Dustproof ring; 4. Rotary sealing ring; 5. Bearing; 6. Primary fixed guide fluid; 7. Bearing sleeve; 8. Upper cup body; 9. Secondary fixed guide fluid; 10. Secondary rotary guide fluid; 11. Upper clamp; 12. Cup body sealing ring; 13. Lower clamp; 14. Lower cup body; 15. Limiting ring; 16. Primary rotary guide fluid. Detailed Implementation

[0020] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. Based on the embodiments of the present utility model, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the protection scope of the present utility model.

[0021] like Figure 1-11As shown, a continuous flow centrifuge cup includes: a bent pipe connector 1, an upper cup body 8, a lower cup body 14, and a rotating connector; the upper cup body 8 and the lower cup body 14 are arranged opposite to each other and sealed together to form a centrifuge chamber. One end of the upper cup body 8 facing away from the lower cup body 14 has a connecting pipe, and the lower end of the bent pipe connector 1 extends into the centrifuge chamber through the connecting pipe; the rotating connector includes a bearing 5 and a bearing sleeve 7. The bearing 5 is installed on the connecting pipe of the upper cup body 8, and the bearing sleeve 7 is installed on the bent pipe connector 1. The bearing sleeve 7 cooperates with the bearing 5 to realize the rotational connection between the upper cup body 8 and the bent pipe connector 1. The top surface of the elbow joint 1 is provided with a liquid outlet, and the side surface of the elbow joint 1 is provided with a liquid inlet. The elbow joint 1 has an inlet flow channel and an outlet flow channel. One end of the inlet flow channel is connected to the inlet, and the other end of the inlet flow channel is connected to the top of the centrifuge chamber. One end of the outlet flow channel is connected to the outlet, and the other end of the outlet flow channel is connected to the bottom of the centrifuge chamber. The upper cup body 8 is fitted with a rotary sealing ring 4 on the connecting pipe. The rotary sealing ring 4 is located between the outer wall of the connecting pipe and the inner wall of the bearing sleeve 7, and is used to achieve a rotary seal between the upper cup body 8 and the bearing sleeve 7.

[0022] Specifically, the inlet channel of the elbow joint 1 includes a horizontal section and a vertical section that are interconnected. The front end of the horizontal section is connected to the inlet, and the rear end of the horizontal section is connected to the upper end of the vertical section. The cross-section of the vertical section is an annular structure, which surrounds the outlet channel. The outlet channel of the elbow joint 1 is vertically arranged, with the upper end of the outlet channel connected to the outlet and the lower end of the outlet channel extending out of the inlet channel.

[0023] Specifically, it also includes: a primary fixed guide fluid 6 and a secondary fixed guide fluid 9; the primary fixed guide fluid 6 and the secondary fixed guide fluid 9 have openings in the middle, both of which are sleeved on the elbow joint 1 and are both interference-fitted to the outer wall of the liquid outlet channel; the primary fixed guide fluid 6 is located between the secondary fixed guide fluid 9 and the bearing sleeve 7.

[0024] Specifically, it also includes: a primary rotating guide fluid 16 and a secondary rotating guide fluid 10; both the primary rotating guide fluid 16 and the secondary rotating guide fluid 10 have holes in the middle, and the lower end of the bent pipe joint 1 passes through the two rotating guide fluids, which can rotate relative to the bent pipe joint 1; the primary rotating guide fluid 16 is fixedly connected to the inner wall of the upper cup body 8, and the edge of the secondary rotating guide fluid 10 is connected to the edge of the primary rotating guide fluid 16.

[0025] Specifically, the upper surface edge of the primary rotating fluid guide 16 is provided with multiple protrusions, which are equidistantly arranged along the circumference. The inner wall of the upper cup body 8 is provided with multiple recesses, which correspond one-to-one with the protrusions and are fixedly connected by adhesive. Except for the protrusions, the primary rotating fluid guide 16 maintains a certain distance from the inner wall of the upper cup body 8 at all other positions, allowing the liquid to pass through smoothly.

[0026] Specifically, the edge of the secondary rotating guide fluid 10 is provided with an annular inner edge, and the annular inner edge has a plurality of locking platforms, and the edge of the primary rotating guide fluid 16 is engaged with the edge of the secondary rotating guide fluid 10.

[0027] Specifically, the upper cup body 8 has a boss structure, which is hollow inside and connected to the connecting pipe. The bearing 5 is sleeved on the connecting pipe of the upper cup body 8, and the inner wall of the bearing 5 is interference-fitted with the outer wall of the connecting pipe. The lower end of the bearing 5 abuts against the boss structure. The primary fixed guide fluid 6 is located in the boss structure of the upper cup body 8 but does not contact the inner wall of the upper cup body 8. The upper surface of the primary rotating guide fluid 16 has an annular protrusion structure, which is inserted into the annular groove of the primary fixed guide fluid 6, and there is a gap between the annular protrusion structure and the groove wall.

[0028] Specifically, the secondary fixed guide fluid 9 is located between the primary rotating guide fluid 16 and the secondary rotating guide fluid 10, and does not contact either the primary rotating guide fluid 16 or the secondary rotating guide fluid 10. The upper surfaces of the primary rotating guide fluid 16, the secondary rotating guide fluid 10 and the secondary fixed guide fluid 9 all gradually slope downward from the center position to the edge position.

[0029] In this embodiment, the rotating guide fluid and the fixed guide fluid do not contact each other. Both rotating guide fluids are connected to the upper cup body 8, together forming the rotatable part of the continuous flow centrifugal separation cup. Both fixed guide fluids are connected to the elbow joint 1, together forming the fixed part of the continuous flow centrifugal separation cup.

[0030] Preferably, the secondary rotating fluid guide 10 is higher than the lower cup body 14. Specifically, an upper clamp 11 is provided on the edge of the upper cup body 8, and a lower clamp 13 is provided on the edge of the lower cup body 14. The upper clamp 11 has a stepped hole in the middle and countersunk through holes around its perimeter. The lower clamp 13 has a stepped hole in the middle and threaded holes around its perimeter. The stepped hole of the lower clamp 13 mates with the stepped hole of the upper clamp 11. The upper clamp 11 and the lower clamp 13 are connected by bolts to fix the lower cup body 14 and the upper cup body 8 together. A cup body sealing ring 12 is provided between the lower cup body 14 and the upper cup body 8. The bottom edge of the upper cup body 8 has a flange and a sealing ring mounting groove. The cup body sealing ring 12 is located in the sealing ring mounting groove. The flange at the bottom of the upper cup body 8 mates with the groove at the top of the lower cup body 14 for fixation. The elbow joint 1 is connected to the bearing sleeve 7 through a flange. A first sealing ring 2 is provided between the flange and the bearing sleeve 7. The first sealing ring 2 is located in the stepped hole at the top of the rotating shaft sleeve 7. The flange presses the first sealing ring 2 tightly.

[0031] Specifically, the rotary sealing ring 4 and the bearing sleeve 7 are sealed by the deformation of the rotary sealing ring 4. Preferably, the rotary sealing ring 4 includes two sealing rings arranged opposite each other, one of which has a flared opening facing upwards and the other has a flared opening facing downwards. Both sealing rings are fitted onto the connecting tube of the upper cup body 8, and seal from the upper and lower directions respectively.

[0032] Specifically, a limiting ring 15 is provided at the end of the connecting pipe. The inner ring of the limiting ring 15 has a stepped hole and is fitted onto the connecting pipe of the upper cup body 8 with an interference fit. The lower end of the limiting ring 15 presses against the rotating sealing ring 4, so that the rotating sealing ring 4 with the trumpet-shaped opening facing downward is pressed against the middle flange of the rotating shaft sleeve 7. The lower step of the rotating shaft sleeve 7 is pressed against the outer ring of the bearing 5 with an interference fit. The rotating sealing ring 4 with the trumpet-shaped opening facing upward is pressed against the lower side of the middle flange of the rotating shaft sleeve 7.

[0033] Specifically, it also includes a dust baffle ring 3. The dust baffle ring 3 has a stepped hole in the middle and a stepped structure around its perimeter. The stepped structure of the dust baffle ring 3 is set on the inner wall of the bearing sleeve 7. The dust baffle ring 3 is located between the limiting ring 15 and the rotary seal ring 4. The edge of the limiting ring 15 presses against the dust baffle ring 3 to prevent the dust baffle ring 3 from shifting. The dust baffle ring 3 is located above the rotary seal ring 4 but does not contact the rotary seal ring 4. The dust baffle ring 3 can prevent the particles generated by the friction of the rotary seal ring 4 from moving upward. The limiting ring 15 can provide secondary blocking for the particles, ensuring that the particles generated by the friction of the rotary seal ring 4 cannot enter the fluid channel.

[0034] Accordingly, this embodiment also provides a cell separation device. Please continue to refer to... Figure 3 The cell separation device includes: an inlet pipe (not shown in the figure), an outlet pipe (not shown in the figure), a rotary power mechanism (not shown in the figure), and a continuous flow centrifugal separation cup as described above; the inlet pipe is connected to the inlet of the bend connector 1, the outlet pipe is connected to the outlet of the bend connector 1, and the rotary power mechanism is connected to the bottom boss of the lower cup body 14. The rotary power mechanism is used to drive the lower cup body 14 and the components fixedly connected to the lower cup body 14 to rotate.

[0035] When the cell separation device is working, the lower cup 14, upper cup 8, primary rotating guide fluid 16, and secondary rotating guide fluid 10 of the rotary power mechanism rotate together, while the bent pipe joint 1, primary fixed guide fluid 6, and secondary fixed guide fluid 9 remain stationary. Figure 4As shown, the cell culture medium flows in through the inlet of the bent connector 1, and flows along the outer channel (i.e., the inlet channel) of the central tube of the bent connector 1 to the top of the primary fixed guide fluid 6. Some of the liquid flows through the gap between the lower surface of the upper cup body 8 and the upper surface of the primary rotating guide fluid 16 to the inner edge of the upper cup body 8. Under the action of centrifugal force, the cells are enriched at the inner edge of the upper cup body 8. Some of the liquid flows through the gap between the primary fixed guide fluid 6 and the primary rotating guide fluid 16 into the gap between the upper surface of the secondary fixed guide fluid 9 and the lower surface of the primary rotating guide fluid 16, and then flows from the edge of the secondary fixed guide fluid 9 to the upper surface of the secondary rotating guide fluid 10, and finally flows to the inner edge of the upper cup body 8. Under the action of centrifugal force, the cells are enriched at the inner edge of the upper cup body 8.

[0036] like Figure 5 As shown, as the cup gradually fills with liquid, the liquid level submerges the inlet channel on the outer layer of the elbow connector 1. The air inside the rotating sealing ring 4 and the bearing sleeve 7 is compressed and balanced with the liquid pressure, causing the liquid level inside the cup to rise further. The air above the rotating sealing ring 4 is compressed within a limited volume, and the compressed air forms a liquid surface isolation zone, which can prevent the rotating sealing ring 4 from contacting the liquid, thereby preventing insoluble particles generated by the high-speed rotation of the rotating sealing ring 4 from entering the liquid and mixing into the cell product, thus contaminating the cell product.

[0037] The continuous flow centrifuge cup provided in this embodiment has a rotary sealing structure located on the outside of the cup body. By using air isolation, the rotary sealing structure is isolated from the cell product concentrate. This prevents insoluble particles generated by the wear of the sealing ring of the rotary sealing structure from contacting the cell product concentrate. This solves the problem of insoluble particles generated by friction of the sealing ring during high-speed rotation in traditional centrifuge cups, where the rotary sealing structure is located inside the cup body. These insoluble particles are mixed in with the cell product during the cell product concentration process. If the content of insoluble particles exceeds the standard, the cell product will be contaminated and unusable, directly leading to the failure of batch production of cell drugs, or requiring additional process steps to remove insoluble particles.

[0038] The continuous flow centrifuge cup provided by this invention employs a multi-stage alternating arrangement of fixed and rotating guide fluids to direct the flow of cell stock solution. This multi-stage guiding process ensures the cell stock solution flows along a predetermined path, guaranteeing the yield of the cell product. Experimental verification shows that using the continuous flow centrifuge cup provided in this embodiment can ensure that over 90% of the liquid flows along the required path, resulting in a cell product yield of over 90%.

[0039] Furthermore, this flow-guiding structure eliminates the need for a rotary seal, avoiding the loss of its auxiliary flow-guiding function after wear. This prevents some cell culture medium from flowing directly out from the center of the flow-guiding structure instead of from the edge to the inner wall of the centrifuge cup, resulting in low cell product yield. Moreover, since the stationary and rotating flow-guiding fluids in the flow-guiding structure do not contact each other, the generation of insoluble particles due to contact friction is effectively avoided.

[0040] In summary, the cell separation device using the continuous flow centrifugal separation cup described above can reduce the risk of cell product spoilage, avoid rework, and reduce time and cost waste; at the same time, it can improve cell product yield, thereby improving economic benefits.

[0041] Finally, it should be noted that the above are merely preferred embodiments of this utility model and are not intended to limit the utility model. Although the utility model has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this utility model should be included within the protection scope of this utility model.

Claims

1. A continuous flow centrifugal separation cup, characterized in that, include: The pipe bend connector (1), the upper cup body (8), the lower cup body (14), and the rotating connector; The upper cup body (8) and the lower cup body (14) are arranged opposite to each other and sealed to form a centrifuge chamber. The upper cup body (8) has a connecting pipe at the end facing away from the lower cup body (14). The lower end of the bent pipe joint (1) extends into the centrifuge chamber through the connecting pipe. The rotating connector includes a bearing (5) and a bearing sleeve (7). The bearing (5) is installed on the connecting pipe of the upper cup body (8), and the bearing sleeve (7) is installed on the bent pipe joint (1). The bearing sleeve (7) cooperates with the bearing (5) to realize the rotating connection between the upper cup body (8) and the bent pipe joint (1). The top surface of the bent pipe joint (1) is provided with a liquid outlet, and the side surface of the bent pipe joint (1) is provided with a liquid inlet. The bent pipe joint (1) is provided with a liquid inlet channel and a liquid outlet channel. One end of the liquid inlet channel is connected to the liquid inlet, and the other end of the liquid inlet channel is connected to the top of the centrifuge chamber. One end of the liquid outlet channel is connected to the liquid outlet, and the other end of the liquid outlet channel is connected to the bottom of the centrifuge chamber. The upper cup body (8) is fitted with a rotating sealing ring (4) on the connecting pipe. The rotating sealing ring (4) is located between the outer wall of the connecting pipe and the inner wall of the bearing sleeve (7) to achieve a rotating seal between the upper cup body (8) and the bearing sleeve (7).

2. The continuous flow centrifugal separation cup according to claim 1, characterized in that, The inlet channel of the elbow joint (1) includes a horizontal section and a vertical section that are interconnected. The front end of the horizontal section is connected to the inlet, and the rear end of the horizontal section is connected to the upper end of the vertical section. The cross-section of the vertical section is an annular structure, and the annular structure surrounds the outlet channel. The outlet channel of the elbow joint (1) is vertically arranged. The upper end of the outlet channel is connected to the outlet, and the lower end of the outlet channel extends out of the inlet channel.

3. The continuous flow centrifugal separation cup according to claim 1 or 2, characterized in that, Also includes: A primary fixed guide fluid (6) and a secondary fixed guide fluid (9); both the primary fixed guide fluid (6) and the secondary fixed guide fluid (9) have a central opening and are sleeved on the elbow joint (1); both the primary fixed guide fluid (6) and the secondary fixed guide fluid (9) are interference-fitted to the outer wall of the elbow joint (1); The primary fixed guide fluid (6) and the secondary fixed guide fluid (9) are both located in the centrifuge chamber, and the primary fixed guide fluid (6) is located between the secondary fixed guide fluid (9) and the bearing sleeve (7).

4. The continuous flow centrifugal separation cup according to claim 3, characterized in that, Also includes: A primary rotating guide fluid (16) and a secondary rotating guide fluid (10); both the primary rotating guide fluid (16) and the secondary rotating guide fluid (10) have holes in the middle, and the lower end of the elbow joint (1) passes through the central holes of the primary rotating guide fluid (16) and the secondary rotating guide fluid (10); The primary rotating guide fluid (16) and the secondary rotating guide fluid (10) are both located in the centrifuge chamber. The primary rotating guide fluid (16) is fixedly connected to the inner wall of the upper cup body (8). The edge of the secondary rotating guide fluid (10) is connected to the edge of the primary rotating guide fluid (16). The secondary fixed guide fluid (9) is located between the primary rotating guide fluid (16) and the secondary rotating guide fluid (10).

5. The continuous flow centrifugal separation cup according to claim 4, characterized in that, The upper surface edge of the primary rotating guide fluid (16) is provided with multiple protrusions, which are equidistantly arranged along the circumferential direction. The inner wall of the upper cup body (8) is provided with multiple recesses, which are corresponding to and fixedly connected to the multiple protrusions. The edge of the secondary rotating guide fluid (10) is provided with an annular inner edge, which has several locking platforms. The edge of the primary rotating guide fluid (16) is engaged with the edge of the secondary rotating guide fluid (10).

6. The continuous flow centrifugal separation cup according to claim 4, characterized in that, The upper cup body (8) has a boss structure, and the lower end of the bearing (5) abuts against the boss structure. The boss structure is hollow inside and communicates with the connecting pipe. The primary fixed guide fluid (6) is located in the boss structure. The upper surface of the primary rotating guide fluid (16) has an annular protrusion structure. The annular protrusion structure is inserted into the annular groove of the primary fixed guide fluid (6), and there is a gap between the annular protrusion structure and the groove wall of the annular groove.

7. The continuous flow centrifugal separation cup according to claim 1, characterized in that, A cup sealing ring (12) is provided between the lower cup body (14) and the upper cup body (8); an upper clamp (11) is provided on the edge of the upper cup body (8), and a lower clamp (13) is provided on the edge of the lower cup body (14). The upper clamp (11) and the lower clamp (13) are connected by bolts; the bent pipe joint (1) is connected to the bearing sleeve (7) through a flange, and a first sealing ring (2) is provided between the flange and the bearing sleeve (7).

8. The continuous flow centrifugal separation cup according to claim 1, characterized in that, The end of the connecting pipe is provided with a limiting ring (15). The inner ring of the limiting ring (15) has a stepped hole and is fitted onto the connecting pipe of the upper cup body (8) with an interference fit. The lower end presses against the rotating sealing ring (4).

9. The continuous flow centrifugal separation cup according to claim 8, characterized in that, It also includes a dustproof ring (3), which is disposed on the inner wall of the bearing sleeve (7) and located between the limiting ring (15) and the rotating sealing ring (4).

10. A cell separation device, characterized in that, include: The continuous flow centrifugal separation cup as described in any one of claims 1 to 9.

Images