A low adsorption liquid chromatography column tube with inner wall mirror polishing

Abstract

The utility model discloses a kind of low adsorption liquid chromatography column pipe of inner wall mirror surface polishing, belong to detection equipment technical field, a kind of low adsorption liquid chromatography column pipe of inner wall mirror surface polishing, including column pipe, the both ends of the column pipe are sealedly connected with liquid inlet connector and liquid outlet connector, microfluidic channel is opened in the pipe wall of the column pipe, the microfluidic channel is fixedly connected with flow guide vane, the top of one end of the column pipe is equipped with liquid inlet port, the bottom of the other end of the column pipe is equipped with liquid outlet port, the liquid inlet port and liquid outlet port are all connected with microfluidic channel, it can effectively solve the " jet effect " problem caused by unreasonable liquid flow entry mode of existing chromatography column pipe, avoid the strong impact to filler bed and sieve plate, improve the temperature stability of column pipe simultaneously, reduce the influence of external environment temperature or mobile phase temperature change on column efficiency, to improve the accuracy of quantitative analysis and the reliability of analysis result.

Description

Technical Field

[0001] This utility model relates to the field of detection equipment technology, and more specifically, to a low-adsorption liquid chromatography column with a mirror-polished inner wall. Background Technology

[0002] In traditional liquid chromatography analysis, the chromatographic column, as the core separation component, has a crucial impact on the accuracy and reproducibility of the analytical results due to the condition of its inner wall surface and its structural design.

[0003] Based on the above, the inventors have discovered that in the prior art, during the use of chromatographic columns, the flow rate increases when the liquid flows from the narrow connecting tube into the thicker column, preventing it from entering the packed bed in a uniform and concentrated manner. This creates a "jet effect," which strongly impacts the packed bed and sieve plate, thus interfering with the accuracy of quantitative analysis. Simultaneously, some chromatographic columns exhibit poor temperature stability; changes in ambient temperature or mobile phase temperature can lead to fluctuations in column efficiency, affecting the reliability of analytical results. Therefore, in view of these issues, the inventors have researched and improved the existing structure to provide a low-adsorption liquid chromatography column with a mirror-polished inner wall, aiming to achieve greater practical value. Utility Model Content

[0004] 1. Technical problems to be solved

[0005] To address the problems existing in the prior art, the purpose of this utility model is to provide a low-adsorption liquid chromatography column with a mirror-polished inner wall. It can effectively solve the "jet effect" problem caused by the unreasonable liquid flow entry method in existing chromatography columns, avoid strong impact on the packing bed and sieve plate, improve the temperature stability of the column, reduce the influence of changes in external ambient temperature or mobile phase temperature on column efficiency, thereby improving the accuracy of quantitative analysis and the reliability of analytical results.

[0006] 2. Technical Solution

[0007] To solve the above problems, the present invention adopts the following technical solution.

[0008] A low-adsorption liquid chromatography column with a mirror-polished inner wall includes a column tube with an inlet connector and an outlet connector sealed at both ends. Microchannels are formed within the column tube wall, and guide vanes are fixedly connected within the microchannels. An inlet port is installed at the top of one end of the column tube, and an outlet port is installed at the bottom of the other end. Both the inlet and outlet ports are connected to the microchannels. The inlet connector contains, in sequence along the inlet direction, a first inlet direct flow channel, a first cylindrical buffer chamber, a second conical buffer chamber, a second inlet direct flow channel, a second conical diffusion chamber, and a pre-filter chamber. The outlet connector contains, in sequence along the outlet direction, a second cylindrical buffer chamber and an outlet direct flow channel. Sieve plates are installed inside the pre-filter and post-filter chambers. The column tube is filled with silica gel packing.

[0009] Furthermore, both ends of the column tube are fixedly connected with pressure caps, and the inlet and outlet connectors are both fixed to the ends of the column tube by pressure caps.

[0010] Furthermore, an inlet pipe is installed at the end of the inlet connector, and an outlet pipe is installed at the end of the outlet connector.

[0011] Furthermore, the inlet and outlet connectors are provided with multiple sealing grooves on their outer periphery, and sealing rings are installed in the sealing grooves. The inlet and outlet connectors are sealed to the inner wall of the column tube through the corresponding sealing rings.

[0012] Furthermore, the microchannel is filled with a constant-temperature liquid, and the guide vanes have a threaded structure.

[0013] Furthermore, the inner wall of the conical diffusion cavity is fixedly connected with multiple guide ribs.

[0014] Furthermore, the column tube is connected to the first liquid inlet channel, the first cylindrical buffer chamber, the first conical buffer chamber, the second liquid inlet channel, the second conical diffusion chamber, the first pre-filter chamber, the second post-filter chamber, the second cylindrical buffer chamber, and the second liquid outlet channel.

[0015] 3. Beneficial Effects

[0016] Compared with existing technologies, the advantages of this utility model are:

[0017] (1) This scheme, through the liquid inlet direct channel one, cylindrical buffer chamber one, conical buffer chamber, liquid inlet direct channel two, conical diffusion chamber and pre-filter chamber set inside the liquid inlet connector, can perform multi-stage buffering and diffusion treatment on the incoming liquid flow. When the liquid flow enters the liquid inlet direct channel one from the liquid inlet pipe, it first enters the cylindrical buffer chamber one for preliminary buffering to reduce the flow rate. Then, it passes through the conical buffer chamber to further stabilize the liquid flow and reduce turbulence. Subsequently, it enters the conical diffusion chamber through the liquid inlet direct channel two. Under the action of the guide ribs, the liquid flow is uniformly guided to the pre-filter chamber, which effectively avoids the "jet effect" caused by the liquid flow directly impacting the packing bed and sieve plate in the traditional chromatographic column tube. This allows the liquid flow to enter the silica gel packing inside the column tube in a more uniform and stable state, ensuring the stability of the separation process and thus improving the accuracy of quantitative analysis.

[0018] (2) In this scheme, a constant-temperature liquid is filled into a microchannel opened inside the column tube wall, and a spiral-shaped guide vane is fixedly connected inside the microchannel. When the constant-temperature liquid enters the microchannel from the inlet port, it can flow along the spiral path under the guidance of the guide vane, and fully exchange heat with the inside of the column tube, thereby keeping the internal temperature of the column tube stable. Even if the external ambient temperature or the mobile phase temperature changes, the constant-temperature liquid can quickly adjust the column temperature, reduce the impact of temperature fluctuations on column efficiency, and thus improve the reliability of the analytical results. Attached Figure Description

[0019] Figure 1 This is a three-dimensional structural diagram of the present invention;

[0020] Figure 2 This is a cross-sectional structural diagram of the present invention;

[0021] Figure 3 This is a schematic diagram of the internal structure of the liquid inlet connector of this utility model;

[0022] Figure 4 This is a schematic diagram of the internal structure of the liquid outlet connector of this utility model.

[0023] Explanation of the labels in the diagram:

[0024] 1. Column tube;

[0025] 2. Liquid inlet connector;

[0026] 3. Liquid outlet connector;

[0027] 4. Microchannels;

[0028] 5. Guide vanes;

[0029] 6. Liquid inlet port;

[0030] 7. Liquid outlet port;

[0031] 8. One direct liquid inlet channel;

[0032] 9. Cylindrical buffer cavity one;

[0033] 10. Conical buffer cavity;

[0034] 11. Liquid inlet direct flow channel two;

[0035] 12. Conical diffuser cavity;

[0036] 13. Pre-filter chamber;

[0037] 14. Post-filter chamber;

[0038] 15. Two cylindrical buffer chambers;

[0039] 16. Direct flow channel for liquid discharge;

[0040] 17. Silica gel filler;

[0041] 18. Press the cap;

[0042] 19. Liquid inlet pipe;

[0043] 20. Discharge pipe;

[0044] 21. Sieve plate;

[0045] 22. Sealing ring;

[0046] 23. Guide ribs. Detailed Implementation

[0047] The technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present utility model. Obviously, the described embodiments are only some embodiments of the present utility model, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present utility model without creative effort are within the protection scope of the present utility model.

[0048] Example:

[0049] Please see Figures 1-4A low-adsorption liquid chromatography column with a mirror-polished inner wall includes a column tube 1. Both ends of the column tube 1 are sealed and connected to an inlet connector 2 and an outlet connector 3, respectively. Microchannels 4 are formed inside the wall of the column tube 1, and guide vanes 5 are fixedly connected inside the microchannels 4. An inlet port 6 is installed at the top of one end of the column tube 1, and an outlet port 7 is installed at the bottom of the other end of the column tube 1. Both the inlet port 6 and the outlet port 7 are connected to the microchannels 4. Inside the inlet connector 2, along the inlet direction, are sequentially formed an inlet direct current channel 8, a cylindrical buffer chamber 9, a conical buffer chamber 10, and an inlet direct current channel 11. The column tube 1 is equipped with a second channel 11, a conical diffusion chamber 12, and a pre-filter chamber 13. Inside the liquid outlet connector 3 and along the liquid outlet direction, there are sequentially arranged a post-filter chamber 14, a cylindrical buffer chamber 15, and a liquid outlet direct channel 16. A sieve plate 21 is installed inside the pre-filter chamber 13 and the post-filter chamber 14. The inside of the column tube 1 is filled with silica gel filler 17. During use, the inner wall of the column tube 1 is treated with a mirror polishing process, which can significantly reduce the roughness of the inner wall surface, reduce the non-specific adsorption of sample molecules in the liquid flow to the inner wall of the column tube 1, thereby reducing sample loss and improving detection sensitivity and accuracy of analysis results.

[0050] See Figure 1 Both ends of the column tube 1 are fixedly connected with pressure caps 18, and the liquid inlet connector 2 and the liquid outlet connector 3 are both fixed to the ends of the column tube 1 by pressure caps 18.

[0051] See Figure 2 The end of the liquid inlet connector 2 is equipped with a liquid inlet pipe 19, and the end of the liquid outlet connector 3 is equipped with a liquid outlet pipe 20.

[0052] See Figure 3 and Figure 4 Multiple sealing grooves are provided on the outer periphery of both the liquid inlet connector 2 and the liquid outlet connector 3, and sealing rings 22 are installed in the sealing grooves. The liquid inlet connector 2 and the liquid outlet connector 3 are sealed to the inner wall of the column tube 1 through the corresponding sealing rings 22. In use, the liquid inlet connector 2 and the liquid outlet connector 3 are sealed to the inner wall of the column tube 1 through the sealing rings 22. With the fixing effect of the pressure cap 18, the overall sealing performance of the column tube 1 is effectively enhanced, preventing liquid leakage and ensuring the normal operation of the chromatographic analysis process.

[0053] See Figure 2 The microchannel 4 is filled with a constant-temperature liquid, and the guide vanes 5 have a spiral structure.

[0054] See Figure 3 Multiple guide ribs 23 are fixedly connected to the inner wall of the conical diffusion cavity 12. During use, the guide ribs 23 can be used to guide the liquid flow evenly to the pre-filter cavity 13.

[0055] See Figure 3 and Figure 4The column tube 1 is connected to the first liquid inlet channel 8, the first cylindrical buffer chamber 9, the first conical buffer chamber 10, the second liquid inlet channel 11, the second conical diffusion chamber 12, the first filter chamber 13, the second filter chamber 14, the second cylindrical buffer chamber 15, and the second liquid outlet channel 16.

[0056] Working Principle: In operation, the mobile phase is first introduced into the column tube 1 through the inlet connector 2. The liquid flows sequentially through the inlet direct flow channel 8, the cylindrical buffer chamber 9, the conical buffer chamber 10, the second inlet direct flow channel 11, the conical diffusion chamber 12, and the pre-filter chamber 13. After being filtered by the sieve plate 21, it enters the silica gel packing 17 inside the column tube 1. During the liquid flow through the silica gel packing 17, different components in the sample undergo adsorption, desorption, and distribution on the silica gel packing 17, achieving separation. The separated liquid flows into the outlet connector 3, sequentially through the post-filter chamber 14, the second cylindrical buffer chamber 15, and the outlet direct flow channel 16, and finally flows out from the outlet pipe 20. Simultaneously, a constant-temperature liquid is introduced into the microchannel 4 through the inlet port 6. Guided by the guide vanes 5, the constant-temperature liquid flows along the spiral path, fully exchanging heat with the inside of the column tube 1 to maintain a stable internal temperature. During this process, the mirror polishing treatment of the inner wall of column tube 1 reduces the non-specific adsorption of sample molecules, the sealing ring 22 ensures the sealing of each connection part, the pressure cap 18 stabilizes the connection between the liquid inlet connector 2 and the liquid outlet connector 3 and column tube 1, and the guide rib 23 ensures that the liquid flows evenly into the pre-filter chamber 13, which together improves the accuracy and reliability of chromatographic analysis.

[0057] Finally, it should be noted that in the description of this utility model, the terms "vertical," "upper," "lower," "horizontal," etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this utility model and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this utility model.

[0058] In the description of this utility model, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal connection of two components. Those skilled in the art can understand the specific meaning of the above terms in this utility model according to the specific circumstances.

[0059] The above description is merely a preferred embodiment of this utility model; however, the protection scope of this utility model is not limited thereto. Any equivalent substitutions or modifications made by those skilled in the art within the technical scope disclosed in this utility model, based on the technical solution and its improved concept, should be included within the protection scope of this utility model.

Claims

1. A low-adsorption liquid chromatography column with a mirror-polished inner wall, comprising a column tube (1), characterized in that: The column tube (1) is sealed at both ends with an inlet connector (2) and an outlet connector (3). A microchannel (4) is formed inside the wall of the column tube (1). A guide vane (5) is fixedly connected inside the microchannel (4). An inlet port (6) is installed at the top of one end of the column tube (1), and an outlet port (7) is installed at the bottom of the other end of the column tube (1). Both the inlet port (6) and the outlet port (7) are connected to the microchannel (4). The inlet connector (2) is located inside the column tube (2) along the inlet direction. The liquid inlet direct flow channel 1 (8), cylindrical buffer chamber 1 (9), conical buffer chamber (10), liquid inlet direct flow channel 2 (11), conical diffusion chamber (12) and pre-filter chamber (13) are sequentially provided. The liquid outlet connector (3) is provided with a rear filter chamber (14), cylindrical buffer chamber 2 (15) and liquid outlet direct flow channel (16) sequentially provided inside and along the liquid outlet direction. The pre-filter chamber (13) and the rear filter chamber (14) are equipped with sieve plates (21). The column tube (1) is filled with silica gel filler (17).

2. The low-adsorption liquid chromatography column with mirror-polished inner wall according to claim 1, characterized in that: Both ends of the column tube (1) are fixedly connected with pressure caps (18), and the liquid inlet connector (2) and the liquid outlet connector (3) are both fixed to the ends of the column tube (1) by pressure caps (18).

3. The low-adsorption liquid chromatography column with mirror-polished inner wall according to claim 1, characterized in that: The inlet connector (2) is equipped with an inlet pipe (19) at its end, and the outlet connector (3) is equipped with an outlet pipe (20) at its end.

4. The low-adsorption liquid chromatography column with mirror-polished inner wall according to claim 1, characterized in that: The inlet connector (2) and outlet connector (3) are provided with multiple sealing grooves on their outer periphery, and sealing rings (22) are installed in the sealing grooves. The inlet connector (2) and outlet connector (3) are sealed to the inner wall of the column tube (1) through the corresponding sealing rings (22).

5. The low-adsorption liquid chromatography column with mirror-polished inner wall according to claim 1, characterized in that: The microchannel (4) is filled with a constant temperature liquid, and the guide vane (5) has a threaded structure.

6. The low-adsorption liquid chromatography column with mirror-polished inner wall according to claim 1, characterized in that: The inner wall of the conical diffusion cavity (12) is fixedly connected with multiple guide ribs (23).

7. The low-adsorption liquid chromatography column with mirror-polished inner wall according to claim 1, characterized in that: The column tube (1) is connected to the first liquid inlet channel (8), the first cylindrical buffer chamber (9), the first conical buffer chamber (10), the second liquid inlet channel (11), the second conical diffusion chamber (12), the first filter chamber (13), the second filter chamber (14), the second cylindrical buffer chamber (15), and the second liquid outlet channel (16).

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