Imprinting Equipment

Abstract

The imprinting apparatus includes a silicon master and an anti-sticking layer coating the silicon master. The silicon master includes a plurality of features arranged at an average pitch of less than about 425 nm, each of the plurality of features including a recess having an opening whose maximum opening dimension is less than about 300 nm. The anti-sticking layer includes a crosslinked silane polymer network.

Description

[Technical field]

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS This application claims the benefit of U.S. Provisional Application No. 63 / 000,964, filed March 27, 2020, the contents of which are incorporated by reference herein in their entirety. [Background technology]

[0002] Nanoimprint technology can enable economical and efficient fabrication of nanostructures. Nanoimprint lithography uses the direct mechanical deformation of a resist material by a stamp bearing nanostructures. The resist material is hardened while the stamp is in place to fix the shape of the nanostructures into the resist material.

[0003] Nanoimprint lithography has been used to produce patterned substrates that can be used in a variety of applications. Some patterned substrates contain fluidic channels and discrete wells or depressions. These patterned substrates may also be incorporated into flow cells. In some flow cells, active surface chemistries are introduced into the discrete depressions while the interstitial regions surrounding the discrete depressions remain inactive. These flow cells may be particularly useful for the detection and evaluation of a wide range of molecules (e.g., deoxyribonucleic acid (DNA)), families of molecules, gene expression levels, or single nucleotide polymorphisms. Summary of the Invention [Means for solving the problem]

[0004] In a first aspect, an imprinting apparatus comprises a silicon master including a plurality of features arranged with an average pitch of less than about 425 nm, each of the plurality of features including an indentation having an opening whose maximum opening dimension is less than about 300 nm, and an anti-stick layer coating a surface of the silicon master, the anti-stick layer comprising a crosslinked silane polymer network.

[0005] In an example of the first embodiment, less than about 0.15% of the total number of the plurality of features have film defects.

[0006] In an example of the first embodiment, the anti-stick layer comprises a first silane monomer:

[0007] [ka]

[0008] and a formulation containing less than about 1.4 wt. % of a second silane monomer containing a hydrolyzable chloride group, based on the total amount of silane present in the formulation.

[0009] In an example of the first embodiment, the anti-stick layer coats the walls of each of the recesses without capping the openings or filling the recesses completely.

[0010] In an example of the first embodiment, the maximum opening dimension of each of the plurality of features ranges from about 170 nm to about 290 nm.

[0011] In an example of the first embodiment, the maximum opening dimension of each of the plurality of features ranges from about 200 nm to about 250 nm.

[0012] In an example of the first embodiment, the average pitch is in the range of about 340 nm to about 410 nm.

[0013] In an example of the first embodiment, the average pitch is in the range of about 350 nm to about 400 nm.

[0014] It should be understood that any of the features of the imprint apparatus disclosed herein may be combined together in any desired manner and / or configuration to achieve the advantages described in the present disclosure, including, for example, generating small, high-resolution features in a fabrication stamp produced using the imprint apparatus and / or facilitating clean release of a fabrication stamp produced from the imprint apparatus.

[0015] In a second aspect, the method includes applying a formulation to a silicon master including a plurality of features arranged with an average pitch of less than about 425 nm, each of the plurality of features having a maximum opening dimension that is less than about 300 nm, the formulation comprising a first silane monomer

[0016] [ka]

[0017] and less than about 1.4 wt %, based on the total amount of silane present in the formulation, of a second silane monomer containing a hydrolyzable chloride group, and curing the applied formulation, thereby forming the anti-stick layer.

[0018] In an example of the second embodiment, the second silane monomer is

[0019] [ka]

[0020] In some of these examples, the formulation includes about 0.28 wt % to about 1.1 wt % of the second silane monomer.

[0021] In an example of the second aspect, the formulation further comprises i) a dimer comprising two bonded units of the first silane monomer, ii) a trimer comprising three bonded units of the first silane monomer, iii) a polymeric species comprising more than three bonded units of the first silane monomer, iv) a first silane monomer having at least one hydrolyzed ethoxy group, or v) any combination of i, ii, iii, and iv.

[0022] In examples of the second embodiment, the curing step includes an incubation process, a post-incubation rinsing process, and a post-rinsing heating process. In some of these examples, the incubation and heating processes are carried out independently at a temperature ranging from about 20° C. to about 250° C. for a time ranging from about 1 minute to about 30 minutes.

[0023] Examples of the second embodiment further include diluting the pure silane material, including the first and second silane monomers, with a first solvent to form a precursor solution, exposing the precursor solution to a basic aqueous solution to produce an aqueous phase and an organic phase, the organic phase including the first solvent, removing the aqueous phase, removing the first solvent from the organic phase to obtain a purified silane material, and diluting the purified silane material with a second solvent to produce a formulation. In some of these examples, the second solvent is an aprotic solvent that i) solvates the purified silane material and ii) has a boiling point in the range of about 50° C. to about 250° C. In some of these examples, the purified silane material is present in the formulation in an amount in the range of about 1 wt % to about 20 wt %.

[0024] In an example of the second embodiment, the step of applying the formulation comprises spin-coating.

[0025] In an example of the second embodiment, the formulation is one of solvent-free or includes from about 1 wt % to about 20 wt % of the first silane monomer.

[0026] It should be understood that any features of the present method may be combined together in any desired manner. Further, it should be understood that any combination of features of the method and / or imprint apparatus may be used and / or combined with any of the examples disclosed herein to achieve the advantages described in this disclosure, including, for example, generating small, high resolution features in an imprint stamp and / or working stamp produced using the imprint apparatus, and / or facilitating clean release of a working stamp produced from the imprint apparatus.

[0027] In a third embodiment, another method includes depositing a formulation onto a silicon master including a plurality of features arranged with an average pitch of less than about 425 nm, each of the plurality of features having a maximum opening dimension of less than about 300 nm, the formulation comprising a first silane monomer:

[0028] [ka]

[0029] and less than 1.4 wt % of a second silane monomer comprising a hydrolyzable chloride group, based on the total amount of silane present in the formulation; forming the imprint device by curing the formulation, thereby forming an anti-stick layer on the silicon master, depositing a silicon-based fabrication stamp material on the anti-stick layer of the imprint device, curing the silicon-based fabrication stamp material to form a fabrication stamp including a negative replica of the plurality of features, and peeling the fabrication stamp from the imprint device.

[0030] In some examples of the third aspect, the step of curing the formulation includes an incubation process, a post-incubation rinsing process, and a post-rinsing heating process. In some of these examples, the incubation and heating processes are carried out independently at a temperature ranging from about 20° C. to about 250° C. for a time ranging from about 1 minute to about 30 minutes.

[0031] In an example of the third embodiment, the silicon-based fabrication stamp material includes a silicon acrylate monomer.

[0032] It should be understood that any features of the present methods may be combined together in any desired manner. Further, it should be understood that any combination of features of the present methods and / or other methods and / or imprint apparatuses may be used and / or combined with any of the examples disclosed herein to achieve the advantages described in this disclosure, including, for example, generating small, high resolution features in an imprint stamp and / or working stamp produced using the imprint apparatus and / or facilitating clean release of a working stamp produced from the imprint apparatus.

[0033] It should further be understood that any features of any of the methods and / or any of the imprint apparatus may be combined together in any desired manner and / or in combination with any of the examples disclosed herein to achieve at least the advantages described herein.

[0034] Features of examples of the present disclosure will become apparent by reference to the following detailed description and the drawings in which like reference numerals correspond to similar, though perhaps not identical, components. For the sake of brevity, reference numerals or features having a previously described function may or may not be described with reference to the other drawings in which they appear. [Brief description of the drawings]

[0035] [Figure 1] FIG. 1 is a cross-sectional view of an example of an imprinting apparatus disclosed herein. [Figure 2A] FIG. 1 illustrates a top view of an example of an imprint apparatus including a single silicon master. [Figure 2B] FIG. 13 illustrates a top view of another example of an imprint apparatus including multiple silicon masters. [Figure 3A] An example of the method disclosed herein is shown. [Figure 3B] An example of the method disclosed herein is shown. [Figure 3C] An example of the method disclosed herein is shown. [Figure 3D] An example of the method disclosed herein is shown. [Figure 4A] 13 shows another example of the method disclosed herein. [Figure 4B] 13 shows another example of the method disclosed herein. [Figure 4C] 13 shows another example of the method disclosed herein. [Figure 4D] 13 shows another example of the method disclosed herein. [Figure 5A] FIG. 1 is a black and white reproduction of an originally colorized atomic force microscope (AFM) image of a top view of an area containing many features covered with large film defects. [Figure 5B] 5B is a cross-sectional view of one of the features of FIG. 5A and a transmission electron microscope (TEM) image of some of the membrane defects at the opening of the feature. [Figure 6A] 13A-13D are bright field microscopy images of imprints formed using worked stamps created from an imprint apparatus having anti-stick layers formed using formulations having different levels of hydrolyzable chloride. [Figure 6B] 13A-13D are bright field microscopy images of imprints formed using worked stamps created from an imprint apparatus having anti-stick layers formed using formulations having different levels of hydrolyzable chloride. [Figure 6C] 13A-13D are bright field microscopy images of imprints formed using worked stamps created from an imprint apparatus having anti-stick layers formed using formulations having different levels of hydrolyzable chloride. [Figure 6D] 13A-13D are bright field microscopy images of imprints formed using worked stamps created from an imprint apparatus having anti-stick layers formed using formulations having different levels of hydrolyzable chloride. [Figure 7] It includes three graphs, the top one showing the percentage of film defects in the anti-stick layer versus the hydrolyzable chloride in the formulation used to produce the anti-stick layer, the middle one showing the average number of film defects in the anti-stick layer versus the hydrolyzable chloride in the formulation used to produce the anti-stick layer, and the bottom one showing the average pixel size of film defects in the anti-stick layer versus the hydrolyzable chloride in the formulation used to produce the anti-stick layer. [Figure 8A] 1 is an image of an imprint formed using a working stamp created from an imprinting apparatus having an anti-stick layer produced with an unaged formulation. [Figure 8B] 1 is an image of an imprint formed using a working stamp created from an imprinting apparatus having an anti-stick layer produced with an unaged formulation. [Figure 8C]1 is an image of an imprint formed using a working stamp created from an imprinting apparatus having an anti-stick layer produced with an unaged formulation. [Figure 9A] 1 is an image of an imprint formed using a worked stamp created from an imprinting apparatus having an anti-stick layer created with an aged formulation. [Figure 9B] 1 is an image of an imprint formed using a worked stamp created from an imprinting apparatus having an anti-stick layer created with an aged formulation. [Figure 9C] 1 is an image of an imprint formed using a worked stamp created from an imprinting apparatus having an anti-stick layer created with an aged formulation. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0036] Patterning techniques have been used to create individual depressions on the surface of a flow cell. The depressions may be functionalized with, for example, capture primers. Within each functionalized depression, a monoclonal population of amplicons (localized and independent clusters) can be generated from each gene oligonucleotide fragment. Higher cluster density can be obtained when the depression dimensions are small and the pitch (e.g., the distance from the center of one depression to the center of the adjacent or nearest depression, or the distance from the edge of one depression to the edge of the adjacent or nearest depression) is small. Higher cluster density means that more bases can be read from a given unit area, thereby increasing gene yield from the patterned flow cell.

[0037] Nanoimprint lithography (NIL) is an example of a high-throughput patterning technique that allows for higher accuracy and lower cost than patterning techniques that use, for example, photons or electrons. NIL utilizes a fabrication stamp to create features (e.g., indentations for a flow cell) in an imprintable material (e.g., a curable resin). The pattern on the fabrication stamp is a negative replica of the desired features to be formed. The fabrication stamp may be created from a master template that has the pattern of the desired features. Some master templates have an anti-stick layer to aid in clean transfer of the pattern to the fabrication stamp material and clean peeling of the fabrication stamp from the master template.

[0038] Some anti-stick layers are produced by depositing and curing a formulation that includes a pure silane material on the surface of the master template. The inventors have discovered that some pure silane materials can produce undesirable defects in the features and / or feature openings of the master template when the feature opening dimensions are less than about 300 nm and the pitch is less than about 425 nm. The defects are films or membranes that may form during heating and may not be easily removed by rinsing. The defects are referred to herein as "membrane defects." The membrane defects may clog (fill) the feature or cap the feature opening. The anti-stick layer can be conformally coated, such as in one case, coating the walls of the feature, thus taking the same shape as the feature and occupying some of the space of the feature opening. However, capping refers to the case where the anti-stick layer extends beyond the conformal coating such that it extends over the remaining feature opening and at least partially (e.g., partially or completely) blocks the feature opening. Filled or capped features may partially or completely prevent replication of these features during fabrication of the working stamp.

[0039] Without being bound to any particular theory, the inventors believe that the formation of these film defects is at least partially due to the presence of a particular silane monomer in the pure silane material. This particular silane monomer contains a hydrolyzable chloride group (e.g., Si-Cl). The hydrolyzable chloride group can affect the solution and deposition properties of formulations containing the pure silane material. The hydrolysis rate of the Si-Cl group is faster than that of the Si-OCH2CH3 group present in the desired silane monomer in the pure silane material. Thus, the Si-Cl group can increase the sensitivity of the formulation to water introduced during formulation preparation. Hydrolysis is the first step toward polymerization of silanes to higher molecular weight species such as dimers, trimers, other higher molecular weight species, and highly crosslinked infinite polymer networks, the latter of which constitute the cured anti-stick layer. The Si-Cl group rapidly forms silanols and acid (e.g., hydrochloric acid (HCl)) by-products. Hydrolysis reactions and subsequent reactions with adjacent reactive groups can be catalyzed by acid, further reducing the stability of the solution and increasing the rate of formation of high molecular weight species. Drying can result in the concentration of loosely bound dimers, trimers and / or high molecular weight species, which continue to polymerize and crosslink rapidly. Receding edges of the droplets can leave films or membranes in or on the features (e.g., depressions) of the master template, which can result in membrane defects.

[0040] In the examples disclosed herein, the formulation that is deposited and cured to produce the anti-stick layer comprises a first silane monomer:

[0041] [ka]

[0042] (containing a slow-polymerizing Si-OCH2CH3 group), and about 1.4 wt% or less of a second silane monomer containing a hydrolyzable chloride group, based on the total amount of silane present in the formulation. The resulting anti-stick layer contains i) the reacted first silane monomer and its high molecular weight adduct, and ii) a crosslinked silane polymer network formed from a minimal amount of the reacted second silane monomer. Any unreacted monomers, dimers, trimers and high molecular weight species that are still soluble are washed away and therefore are not part of the crosslinked silane polymer network. Because the formulation used to produce the anti-stick layer has about 1.4 wt% or less of the second silane monomer, based on the total amount of silane present, minimal or no film defects are formed. In some cases, the anti-stick layer does not have film defects and can therefore be considered defect-free. In other cases, the anti-stick layer may contain minimal film defects, for example less than 0.15% of the recesses have film defects. In some cases, it is less than 0.14%, or less than 0.13%, or less than 0.12%, or less than 0.11%, or less than 0.10%, or less than 0.09%, or less than 0.08%, or less than 0.07%, etc. In any of these cases, the lower limit is greater than zero. In these cases, the anti-stick layer can be considered to be substantially defect-free. The phrase "at least substantially defect-free" means that the anti-stick layer is defect-free or substantially defect-free.

[0043] The at least substantially defect-free anti-stick layer of the imprinting apparatus disclosed herein contributes to a clean transfer of the pattern of features to the fabrication stamp material, which in turn results in the controlled formation of features having small dimensions and arranged at a narrow (small) pitch. As mentioned, this can be particularly desirable for the recesses of the flow cell, as gene yield from a patterned flow cell can be increased.

[0044] definition It should be understood that terms used herein take their ordinary meaning in the relevant art unless otherwise specified. Some terms used herein and their meanings are set forth below.

[0045] The singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise.

[0046] The terms comprising, including, containing, and various forms of these terms are synonymous with each other and are intended to be of similar broad scope.

[0047] Terms such as top, bottom, lower, upper, on, etc. are used herein to describe the flow cell and / or various components of the flow cell. It should be understood that these directional terms do not imply a particular orientation, but are used to specify the relative orientation between components. The use of directional terms should not be construed to limit the examples disclosed herein to any particular orientation.

[0048] The term "anti-stick layer" as used herein refers to a film that includes a cross-linked silane polymer network. The monomer and its higher molecular weight adducts (dimers, trimers, etc.) that polymerize and cross-link to form a cross-linked silane polymer network are referred to herein as first silane monomers (see structures below). The formulations used in producing the anti-stick layer may also include small amounts of what are referred to herein as second silane monomers (see structures below). The second silane monomers may polymerize and cross-link to form a small portion of the cross-linked silane polymer network. Any unreacted monomers and loosely bound high molecular weight species that remain soluble in the formulation or washing solvent are removed during processing and are therefore not present in the anti-stick layer. The anti-stick layer is also capable of bonding to the underlying substrate.

[0049] The term "membrane defect" refers to a thin film or membrane of an anti-stick layer that fills and thus clogs a feature in the imprinting apparatus or that is disposed over and thus caps some or all of the feature opening.

[0050] The term "depositing" as used herein refers to any suitable application technique, which may be manual or automated, and in some cases results in the modification of surface properties. In general, deposition can be performed using deposition techniques, coating techniques, grafting techniques, etc. Some specific examples include chemical vapor deposition (CVD), spray coating (e.g., ultrasonic spray coating), spin coating, dunk or dip coating, doctor blade coating, paddle dispensing, flow-through coating, aerosol printing, screen printing, microcontact printing, inkjet printing, etc.

[0051] As used herein, the term "depression" refers to a discrete concave feature of a substrate, patterned resin, etc., having a surface opening at least partially surrounded by a void region of the substrate, resin, etc. A depression can have any of a variety of shapes at its opening in the surface, including, by way of example, round, elliptical, square, polygonal, star-shaped (having any number of vertices), etc. A cross-section of a depression taken perpendicular to the surface may be curved, square, polygonal, hyperbolic, conical, angular, etc.

[0052] The term "each," when used in reference to a collection of items, is intended to identify each individual item in the collection, but does not necessarily refer to every item in the collection. Exceptions may occur where express disclosure or context clearly dictates otherwise.

[0053] A "feature" as used herein refers to a point or area in a pattern that can be distinguished from other points or areas depending on their relative position. Exemplary features include depressions in a substrate, protrusions from a substrate, bumps on a substrate, etc. In one example, each of a plurality of features of an imprint apparatus is a depression.

[0054] The "first silane monomer" is

[0055] [ka]

[0056] The formulation used to produce the anti-stick layer may also include a hydrolyzed form of the first silane monomer where one or more of the ethoxy groups have been hydrolyzed (CH3CH20-Si→HO-Si). This intermediate monomer is utilized for the polymerization of the first silane monomer into a high molecular weight cross-linked polymer network.

[0057] The phrases "formed from" and "produced with" may be used herein with reference to the anti-stick layer and its precursor formulation. As an example, the anti-stick layer may be formed from the formulation. As another example, the anti-stick layer may be produced with the formulation. In either case, "formed from" or "produced with" is intended to mean that the formulation is used during processing to create the anti-stick layer. These terms do not mean that all of the formulation components become components of the anti-stick layer. For example, solvents, unreacted monomers, and high molecular weight species that remain soluble in the formulation or cleaning solutions that may be present in the formulation are not part of the final anti-stick layer.

[0058] As used herein, the term "flow cell" refers to a vessel having a chamber (e.g., a flow path) in which a reaction can occur, an inlet for delivering reagents to the chamber, and an outlet for removing reagents from the chamber. In some cases, the chamber allows for detection of a reaction occurring within the chamber. For example, the chamber / flow path may include one or more transparent surfaces that allow for optical detection of an array of wells, optically labeled molecules, etc.

[0059] "High molecular weight species or adducts" are polymeric units that contain more than three linked monomeric units. "Dimer" refers specifically to polymeric species having two linked monomeric units. "Trimer" refers specifically to polymeric species having three linked monomeric units. Some dimers, trimers and high molecular weight species polymerize and crosslink to form a crosslinked silane polymer network. Other dimers, trimers and high molecular weight species may remain soluble in the formulation or wash solution and therefore are not incorporated into the crosslinked silane polymer network.

[0060] The term "imprint defect" refers to a portion of the imprint resin or material to which a feature has been transferred from the imprint apparatus due to a film defect present in the imprint apparatus.

[0061] As used herein, the term "gap region" refers to an area of ​​a surface (e.g., of a substrate, patterned resin, etc.) that separates features. For example, a gap region can separate one feature of an array from another feature of an array or pattern. Two features that are separated from each other may be discrete, i.e., lack physical contact with each other. In another example, a gap region can separate a first portion of a feature from a second portion of the feature. In many examples, the gap region is continuous, whereas the features are discrete, such as in the case of multiple depressions defined in an otherwise continuous surface. The separation provided by the gap region may be partial or complete separation. The gap region may have a surface material that is different from the surface material of the features defined on the surface. For example, a feature of a flow cell array may have an amount or concentration of polymer coating and primer that exceeds the amount or concentration present in the gap region. In some examples, the polymer coating and primer may not be present in the gap region.

[0062] As used herein, the term "pure silane material" refers to silane monomers, and in some cases higher molecular weight species or adducts thereof, without any solvents, catalysts, initiators, or other added components. In some examples, the pure silane material may consist of a first silane monomer and less than about 1.4 wt% of a second silane monomer, based on the total amount of silanes present in the pure silane material. In other examples, the pure silane material may consist of a first silane monomer, a hydrolyzed form of the first silane monomer, and less than about 1.4 wt% of the second silane monomer, based on the total amount of silanes present in the pure silane material. In addition to the first silane monomer, some examples of pure silane materials may also include dimers, trimers, and / or other higher molecular weight species or adducts of the first silane monomer. In one example, the pure silane material comprises about 65 wt% to about 100 wt% of the first silane monomer, and about 0 wt% to about 35 wt% of dimers, trimers and / or other high molecular weight species or adducts of the first silane monomer. The amount of the second silane monomer relative to the total amount of silane present in the pure silane material can be less. For example, the amount can be less than about 2 wt%, e.g., less than about 1.5 wt%, about 1.4 wt%, about 1.3 wt%, about 1.2 wt%, about 1.1 wt%, about 1 wt%, about 0.9 wt%, about 0.8 wt%, about 0.7 wt%, about 0.6 wt%, about 0.5 wt%, about 0.4 wt%, about 0.3 wt%, about 0.2 wt%, about 0.1 wt%, about 0.05 wt%, about 0.02 wt%, about 0.01 wt%, about 0.005 wt%, about 0.002 wt%, about 0.001 wt% or less. In some examples, the pure silane material includes 0 wt% to about 1.25 wt% of the second silane monomer, or about 0.001 wt% to about 1 wt% of the second silane monomer.

[0063] As used herein, the term "pitch" refers to the spacing of features. In one example, pitch refers to the spacing from the center of one feature to the center of an adjacent or nearest feature. This pitch may also be referred to as center-to-center spacing. In another example, pitch refers to the spacing from the right edge of one feature to the left edge of an adjacent or nearest feature. This pitch may also be referred to as edge-to-edge spacing. In one example, when the features are depressions (e.g., wells such as nanowells), "pitch" refers to the center-to-center spacing between two adjacent depressions.

[0064] As used herein, a "primer" is defined as a single-stranded nucleic acid sequence (e.g., single-stranded DNA). Some primers, which may be referred to as amplification primers, serve as starting points for template amplification and cluster generation. Other primers, which may be referred to as sequencing primers, serve as starting points for DNA synthesis. The 5' end of the primer may be modified to allow for coupling reactions with functional groups on a polymer coating. The length of the primer may be any number of bases long and may include a variety of non-natural nucleotides. In one example, the sequencing primer is short, ranging from 10 to 60 bases, or 20 to 40 bases.

[0065] "Second silane monomer" refers to a cyclosiloxane having at least one hydrolyzable chloride group. In one example, the second silane monomer is

[0066] [ka]

[0067] It is.

[0068] Imprinting Equipment An example of an imprint apparatus 10 is shown in Figure 1. The imprint apparatus 10 includes a silicon master 12 including a plurality of features 14 arranged with an average pitch P1 or P2 of less than 425 nm, each of the plurality of features 14 including an indentation having an opening whose maximum opening dimension D is less than about 300 nm, and an anti-stick layer 16 coating the silicon master 12, the anti-stick layer 16 including a crosslinked silane polymer network. In some examples, less than 0.15% of the total number of the plurality of features 14 have film defects.

[0069] Silicon master 12 is a substrate 13 having a pattern of features 14 defined therein. The features 14 may be defined by etching. This pattern is representative of the desired pattern of features 14 in a final product, such as a flow cell. Silicon master 12 serves as a master mold for creating a working stamp or multiple working stamps, each having a negative replica of the pattern, and thus can be used to create the final product.

[0070] In some cases, the substrate 13 of the silicon master 12 is completely patterned with features 14. In these cases, one substrate 13 may have all of the features 14 of the pattern defined thereon. A top view of an example of this imprinting apparatus 10 is shown in FIG. 2A. This imprinting apparatus 10 may be used to create a single working stamp. In other cases, the substrate 13 of the silicon master 12 is patterned with a plurality of separate discrete patterned areas 15. In these cases, each separate discrete patterned area 15 includes a set of features 14. In these cases, the substrate 13 has a respective set of features 14 defined thereon, with each respective set of features 14 defined at a specific location on the substrate 13. A top view of an example of this imprinting apparatus 10' is shown in FIG. 2B. As shown, the features 14 of each patterned area 15 are defined at respective locations across the wafer. This imprinting apparatus 10' may be used to create a plurality of working stamps.

[0071] The substrate 13 of the silicon master 12 may include a layer of silicon, silicon dioxide (SiO2), or any combination thereof.

[0072] As mentioned, silicon master 12 includes features 14 defined in substrate 13. In the example disclosed herein, features 14 are indentations.

[0073] In the examples disclosed herein, the features 14 are arranged with an average pitch P1 or P2 of less than about 425 nm. In some cases, the features 14 are arranged with an average pitch P1 or P2 of less than about 430 nm, about 425 nm, about 420 nm, about 415 nm or less. The pitch P1 in FIG. 1 indicates the center-to-center spacing. The pitch P2 in FIG. 1 indicates the end-to-end spacing. The formulations disclosed herein are subjected to a curing process that produces an anti-stick layer 16 on the surface of the silicon master 12 having features 14 at these narrow pitches without producing clogging or capping film defects as described herein. Thus, the formulations are particularly suitable for the narrow pitch ranges disclosed herein. In some examples, the average pitch P1 or P2 ranges from about 340 nm to about 410 nm. In some other examples, the average pitch P1 or P2 ranges from about 350 nm to about 400 nm.

[0074] The features 14 of a given pattern (e.g., patterned area 15) of silicon master 12 may have any desired layout for the final product. The layout may be a regular, repeating, or non-regular pattern. In one example, the features 14 are arranged in a hexagonal grid for close packing and increased density. Other layouts may include, for example, rectilinear (i.e., rectangular) layouts (e.g., lines or grooves), triangular layouts, etc. In some examples, the layout or pattern may be an xy format of features 14 in rows and columns. In some other examples, the layout or pattern may be a repeating arrangement of features 14 and / or interstitial regions 18. In still other examples, the layout or pattern may be a random arrangement of features 14 and / or interstitial regions 18. The patterns may include stripes, swirls, lines, triangles, rectangles, circles, arcs, checks, diagonals, arrows, squares, etc.

[0075] In the examples disclosed herein, each feature 14 is a depression 14' having an opening 20. The depression 14' can have any of a variety of shapes at its opening 20 in the surface of the silicon master 12, including, by way of example, round, oval, square, polygonal, etc. The maximum dimension of the opening 20 may depend on the shape of the opening 20. For example, if the opening 20 is circular or oval, the maximum dimension D is the diameter or major axis, respectively. As another example, if the opening 20 is square or rectangular, the maximum dimension D is the length. The maximum opening dimension D is less than about 300 nm. In some cases, the maximum opening dimension D is less than about 310 nm, about 305 nm, about 300 nm, about 295 nm, or less. In some examples, the maximum opening dimension D is in the range of about 170 nm to about 290 nm. In some other examples, the maximum opening dimension D is in the range of about 180 nm to about 270 nm, or about 200 nm to about 250 nm.

[0076] The three-dimensional shape within the cavity 14' may resemble a cylinder, a cube, a rectangular solid, a cone, or the like.

[0077] Each feature 14 (eg, depression 14') may also be defined by its volume and / or depth.

[0078] The minimum or maximum feature volume may be selected based on the final product formed using the fabricated stamp formed using imprint apparatus 10 or 10'. For example, if the final product is a flow cell, the volume of features 14 of silicon master 12 may be selected such that the indentation of the final flow cell can accommodate the expected throughput (e.g., multiplexing), resolution, nucleotide, or analyte reactivity of the downstream application of the flow cell. For example, the volume may be at least about 1×10 -3 μm 3 , about 1×10 2 μm 3 , about 0.1μm 3 , about 1μm 3 , about 10μm 3 , about 100μm 3 , or more, or less.

[0079] Similarly, feature depth may be selected based on the final product to be formed using the fabricated stamp formed using imprint apparatus 10 or 10'. For example, if the final product is a flow cell, the depth of features 14 in silicon master 12 may be selected so that the recesses of the final flow cell can accommodate the polymer hydrogel and primer. In one example, the depth may be about 0.1 μm, about 0.5 μm, about 1 μm, about 10 μm, about 100 μm, or more or less. In some examples, the depth is about 0.2 μm or 0.4 μm. The depth of each feature 14 may be greater than, less than, or between the values ​​specified above.

[0080] The anti-stick layer 16 coats the silicon master 12. As shown in FIG. 1, the anti-stick layer 16 conformally coats the exposed surface of the silicon master 12, including along the walls of the features 14 (e.g., recesses 14') and on the interstitial regions 18. In some examples, the anti-stick layer 16 conformally coats the respective walls of the recesses 14' (and thus takes the shape of the recesses 14') without extending up and thus capping the openings 20 or filling the recesses 14'. In these examples, the anti-stick layer 16 does not clog the features 14 / recesses 14' and does not form a capping film covering at least a portion of the openings 20. In all examples disclosed herein, less than 0.15% of the total number of the plurality of features 14 (e.g., recesses 14') have film defects. In other words, less than 0.15% of the total number of the plurality of features 14 are clogged or capped by film defects. In some cases, less than 0.2% of the total number of the plurality of features 14 are clogged or capped by film defects. In some cases, less than 0.15% of the total number of the plurality of features 14 are clogged or capped by film defects. In some cases, less than 0.13% of the total number of the plurality of features 14 are clogged or capped by film defects. In some cases, less than 0.12% of the total number of the plurality of features 14 are clogged or capped by film defects. In some cases, less than 0.11% of the total number of the plurality of features 14 are clogged or capped by film defects. In other cases, less than 0.05% of the total number of the plurality of features 14 are clogged or capped by film defects. A high percentage of film defects results in a high percentage of features 14 that cannot be transferred from the imprint apparatus 10 to the working stamp or from the working stamp to the imprint resin. A reduction in the number of features in the imprinted resin leads to a reduction in the number of wells in which sequencing reactions can take place, which can have a detrimental effect on the sequencing workflow and / or results. In some cases, the final imprinted resin may have too many unusable wells. Even 0.15% film defects are undesirable because they can lead to defects in the film (see, e.g., Example 2 and FIG. 6D). In many cases, less than 0.15% (e.g., less than 0.1%, less than 0.05%, etc.) film defects are desirable.

[0081] The anti-stick layer 16 comprises a crosslinked silane polymer network produced by polymerization and crosslinking of i) a first silane monomer and its high molecular weight adduct, and, if present, ii) a minimal amount of a second silane monomer and its high molecular weight adduct. The anti-stick layer 16 does not include unreacted monomers, dimers, trimers and oligomers that may be part of the formulation used to produce the anti-stick layer 16. These unreacted monomers, dimers, trimers and high molecular weight species may remain soluble in the formulation or in the cleaning solution and are not incorporated into the crosslinked silane polymer network.

[0082] Additionally, the anti-stick layer 16 has a surface energy suitable to support stable wetting of the fabricated stamp material into the thin film on the imprint apparatus 10, 10'. In one example, the surface energy of the anti-stick layer 16 is defined by a water contact angle, which may be in the range of about 70° to about 90°, or about 75° to about 85°.

[0083] Formulation and method of making an imprint device In the examples disclosed herein, the anti-stick layer 16 is a first silane monomer.

[0084] [ka]

[0085] and less than about 1.4 wt% of a second silane monomer comprising a hydrolyzable chloride group, based on the total amount of silane present in the formulation. In these examples, the hydrolyzable chloride may be present in an amount less than about 500 ppm. In some examples, the formulation does not include any second silane monomer. In other examples, the formulation includes from about 0.28 wt% to about 1.1 wt% of the second silane monomer, based on the total amount of silane present in the formulation. In these examples, the hydrolyzable chloride may be present in an amount ranging from about 100 ppm to about 400 ppm. In yet other examples, the formulation includes from about 0.1 wt% to about 1 wt% of the second silane monomer, based on the total amount of silane present in the formulation.

[0086] The first silane monomer and the second silane monomer may be present in a pure silane material. In some examples, the pure silane material consists of the first silane monomer and the second silane monomer. In other examples, the pure silane material may include other species of the first silane monomer. For example, some of the first silane monomers in the pure silane material may have undergone hydrolysis, and thus some of the first silane monomers may include hydrolyzed ethoxy groups. In another example, some of the first silane monomers in the pure silane material may have undergone initial polymerization, and thus some dimers, trimers, or other high molecular weight species may be present in the formation. In one example, the formulation further includes i) a dimer comprising two bond units of the first silane monomer, ii) a trimer comprising three bond units of the first silane monomer, iii) a polymeric species comprising more than three bond units of the first silane monomer, iv) a first silane monomer having at least one hydrolyzed ethoxy group, or v) any combination of i), ii), iii), and iv). In yet other examples, the pure silane material may include the first silane monomer, any one or more of the other species of the first silane monomer, the second silane monomer, and one or more other species of the second silane monomer. The other species of the second silane monomer in the pure silane material may include dimers, trimers, and higher molecular weight species, and / or hydrolysis by-products of the second silane monomer and / or acid by-products of the second silane monomer.

[0087] The pure silane material may be formulated with a first silane monomer and no second silane monomer or with a desired low level of the second silane monomer. In some examples, the pure silane material may be applied as a formulation. The formulations in these examples are solvent-free. In other examples, the pure silane material may be diluted with a solvent. Examples of suitable solvents include any aprotic solvent that i) solvates the pure silane material and ii) has a boiling point in the range of about 50° C. to about 250° C. The boiling point should be low enough to facilitate easy removal during heating, but high enough so that the application process used to deposit the formulation does not result in rapid drying of the applied formulation. Examples of suitable aprotic solvents include tetrahydrofuran (THF), 2-methyltetrahydrofuran (MeTHF), propylene glycol methyl ether acetate (PGMEA), toluene, and the like. The solvent may be used in any suitable amount that results in a formulation that includes about 1 wt % to about 20 wt % of the first silane monomer.

[0088] When the formulation is prepared with a desirably low level of the second silane monomer, it may be desirable to store the formulation for a period of time before applying to form the anti-stick layer 16. Aging the formulation can improve the coatability of the formulation, due in part to initiating hydrolysis of the first silane monomer prior to coating. In one example, the formulation can be stored at room temperature conditions (e.g., 18° C. to about 22° C.) for about 2 days to about 14 days.

[0089] The pure silane material may alternatively be formulated with a first silane monomer and a second silane monomer at a level higher than the desired low level of less than about 1.4 wt%. In these examples, the pure silane material may be treated before being incorporated into the formulation. The treatment can convert Si-Cl to SiOH via hydrolysis and remove the acid by-product (HCl). Since both the first and second silane monomers are hydrolyzed to some extent in the formulation and can react and crosslink with the substrate surface, it may be desirable to convert Si-Cl and remove the HCl by-product before the formulation is produced. The treatment can reduce the amount of the second silane monomer to less than about 1.4 wt% (relative to the total amount of silane present in the pure formulation).

[0090] In these examples, the steps of forming the formulation may include diluting a pure silane material comprising the first and second silane monomers with a first solvent to form a precursor solution, exposing the precursor solution to a basic aqueous solution to produce an aqueous phase and an organic phase, where the organic phase comprises the first solvent, removing the aqueous phase, removing the first solvent from the organic phase to obtain a purified silane material, and diluting the purified silane material with the second solvent.

[0091] As mentioned, if the pure silane material contains a first silane monomer and an undesirably high level of a second silane, the pure silane material may be diluted with a first solvent to form a precursor solution. Any aprotic solvent that solubilizes the silane and is immiscible with water may be used as the first solvent. An example of the first solvent is ethyl acetate.

[0092] The precursor solution is then exposed to a basic aqueous solution to produce an aqueous phase and an organic phase (including the first silane monomer). An example of a basic aqueous solution is a saturated sodium bicarbonate solution. The mixture may be stirred or otherwise mixed for a suitable time to allow hydrolysis of Si-Cl, and then allowed to settle for a time sufficient to separate the aqueous and organic phases. In one example, vigorous mixing is performed for about 10 minutes. The hydrolyzable chloride groups react with the base to form a water-soluble salt, which is removed from the organic phase, for example by washing. The organic phase includes the first silane monomer and the first solvent.

[0093] The aqueous phase may be removed from the organic phase. The aqueous and organic phases will separate gravimetrically as the heavier one settles to the bottom of the vessel. The heavier phase drains out the bottom.

[0094] Additional water may be added to wash the organic phase, and the entire process may be repeated multiple times (e.g., 2-4 times).

[0095] Prior to removing the first solvent from the organic phase, the organic phase may be dried using a drying agent, such as, for example, anhydrous magnesium sulfate. The organic phase may be dried for any suitable period of time, for example, from about 10 minutes to about 30 minutes. The drying agent may be filtered before subjecting the organic phase to further processing.

[0096] The first solvent can then be removed from the organic phase to obtain the purified silane material. Removal of the first solvent can include using a rotary evaporator on the organic phase. In some examples, the purified silane material is a colorless liquid.

[0097] The purified silane material includes a first silane monomer and either no second silane monomer or desirably a low level of the second silane monomer. In this example, the purified silane material may be diluted with a second solvent to produce the formulation. The second solvent may be any aprotic solvent that i) solvates the pure silane material and ii) has a boiling point in the range of about 50° C. to about 250° C. Examples include those mentioned herein such as THF, methyl THF, toluene, etc. The second solvent may be used in any suitable amount such that the purified silane material is present in the formulation in an amount ranging from about 1 wt % to about 20 wt %. In another example, the purified silane material may be present in the formulation in an amount ranging from about 5 wt % to about 15 wt %, such as from about 2 wt % to about 10 wt %.

[0098] Any of the examples of the formulations may also include an inhibitor. One exemplary inhibitor includes butylated hydroxytoluene (BHT), which can be used when THF is the solvent. Other inhibitors may be more suitable for other solvents. The amount of inhibitor may range from about 100 ppm to about 500 ppm. In other examples, the amount of inhibitor may range from about 150 ppm to about 450 ppm, such as from about 125 ppm to about 475 ppm.

[0099] Any of the exemplary formulations may be used to produce the anti-stick layer 16, and thus any of the exemplary imprinting apparatus 10, 10'. One exemplary method of making the imprinting apparatus 10, 10' includes applying a formulation to a silicon master 12 that includes a plurality of features 14 arranged with an average pitch of less than about 425 nm, each of the plurality of features 14 having a maximum opening dimension of about 300 nm, the formulation comprising a first silane monomer:

[0100] [ka]

[0101] and % based on the total amount of silane present in the formulation of a second silane monomer containing a hydrolyzable chloride group, and curing the applied formulation, thereby forming the anti-stick layer 16. This exemplary method is shown in Figures 3A through 3D.

[0102] FIG. 3A shows a silicon master 12 that includes a substrate 13 having features 14 defined therein.

[0103] The formulations used in the method may be obtained (e.g., from a supplier, manufacturer, etc.) or may be prepared as described herein. As described herein, the formulations are solvent-free or include about 1 wt% to about 20 wt% of the first silane monomer. When a solvent is included, the first silane monomer may be present in an amount of 25 wt% or less (e.g., 24 wt%, 23 wt%, 22 wt%, 21 wt%, 20 wt% or less). In other examples, the formulations include about 2.5 wt% to about 15 wt% of the first silane monomer, or about 5 wt% to about 10 wt% of the first silane monomer. In any of the exemplary formulations, the second silane monomer is present in an amount of about 2.0 wt% or less, e.g., about 1.5 wt%, about 1.4 wt%, about 1.2 wt% or less of the total amount of silane present in the formulation. In some examples, the formulation includes about 0.25 wt % to about 1 wt % of the second silane monomer.

[0104] 3B shows the formulation 22 applied to the silicon master 12. The formulation 22 may be applied to the silicon master 12 using any suitable deposition technique. In one example, the application of the formulation comprises spin coating. The spin coating may include a slow step to introduce a puddle of the formulation 22 that is incubated for a limited period of time (e.g., up to 30 seconds). Water (e.g., from a cleaning step of the silicon master 12, from air, etc.) may be introduced into the formulation during its application.

[0105] The step of curing the applied formulation may include one or more processes. In one example, the curing step includes an incubation process, a rinsing process after the incubation process, and a heating process after the rinsing process.

[0106] FIG. 3C illustrates the incubation process. Without being bound by any theory, polymerization and crosslinking of the first silane monomer (and any dimers, trimers, and higher molecular weight species that may be present in the formulation) is initiated during the incubation process. As shown in FIG. 3C, the anti-stick layer 16 begins to form. One possible reaction involves the ethoxy R groups of the first silane monomer, which can react with the surface groups (e.g., silanols) of the silicon master 12 to attach the anti-stick layer 16 to the silicon master 12. Alternatively or additionally, silanols form on the monomer, and these silanols condense with the silanols of the silicon master 12. Another possible reaction is hydrolysis of the CH3CH20-Si groups, which results in condensation and polymerization of the silane into higher molecular weight species, such as dimers, trimers, higher molecular weight species, and highly crosslinked silane polymer networks. Crosslinking may involve condensation of silanols on the monomer with other monomeric silanols, or with ethoxy groups.

[0107] This heating process is carried out at a suitable temperature and for a suitable time to cause polymerization and crosslinking of the first silane monomer to occur.

[0108] After the incubation step, a rinse may be performed. The rinse may be performed with any solvent that solvates the monomers, dimers, trimers and high molecular weight species. Thus, the rinse will wash away any unreacted monomers, dimers, trimers and / or high molecular weight species, leaving behind a cross-linked silane polymer network, i.e., the anti-stick layer 16. The imprint apparatus 10 after rinsing is shown in FIG. 3D.

[0109] The imprint apparatus 10 (silicon master 12 with the anti-stick layer 16 thereon) may then be subjected to a second heating process. This heating process may be performed to dry the anti-stick layer 16 and thus remove any remaining water, solvent, etc. However, it should be understood that cross-linking may continue during this second heating process.

[0110] The first and second heating processes are carried out independently at a temperature ranging from about 18°C ​​to about 260°C for a time period ranging from about 60 minutes. In one example, the first and second heating processes are carried out independently at a temperature ranging from about 20°C to about 250°C for a time period ranging from about 1 minute to about 30 minutes. In one example, the incubation process is accomplished at room temperature (e.g., about 20°C to about 25°C) for about 30 minutes. In another example, the incubation process is accomplished at a temperature ranging from about 100°C to about 250°C, or about 130°C to about 250°C for a time period ranging from about 10 minutes to about 20 minutes. In yet another example, the second heating process is accomplished at a temperature ranging from about 100°C to about 250°C, or about 110°C to about 150°C, or about 130°C to about 250°C for a time period ranging from about 1 minute to about 5 minutes.

[0111] The rate of formation of high molecular weight species from the first silane monomer may be relatively slow so that the rinsing and subsequent heating (drying) process removes unreacted monomer and loosely bound dimers, trimers and / or high molecular weight species before they can polymerize and crosslink. Thus, as the droplet of formulation 22 recedes from feature 14, no film defects are formed in feature 14 and / or over feature opening 20.

[0112] Method for using an imprint device Imprint apparatus 10, 10' may be used to form a working stamp. One example of this method includes depositing a formulation 22 onto a silicon master 12 that includes a plurality of features 14 arranged with an average pitch ranging from about 340 nm to about 410 nm, each of the plurality of features having a maximum opening dimension ranging from about 170 nm to about 290 nm, the formulation 22 comprising a first silane monomer:

[0113] [ka]

[0114] and less than 1.4 wt % of a second silane monomer comprising a hydrolyzable chloride group, based on the total amount of silane present in the formulation, curing formulation 22, thereby forming an anti-stick layer 16 on silicon master 12, depositing a silicon-based fabrication stamp material on the anti-stick layer 16 of imprint device 10, 10', curing the silicon-based fabrication stamp material to form a fabrication stamp including a negative replica of the plurality of features 14, and peeling the fabrication stamp from imprint device 10, 10' to form imprint device 10, 10'. This exemplary method is illustrated in Figures 4A through 4D.

[0115] Figure 4A shows the imprint apparatus 10, 10'. Any example of the imprint apparatus 10, 10' may be used and any method of making the imprint apparatus 10, 10' may be used.

[0116] FIG. 4B illustrates the application of a silicon-based working stamp (WS) material 24. By "silicon-based" material, it is meant that the material is composed of at least about 50 mol% silicon-containing molecules (repeating monomer units). In one example, silicon-based WS material 24 is composed of about 100 mol% silicon-containing molecules (repeating monomer units). In a further example, WS material 24 may be a "silicon-containing polymer" (i.e., a polymer having less than about 50 mol% silicon-containing molecules). In another example, WS material 24 includes polymerized silicon acrylate or methacrylate monomers. In another example, WS material 24 also includes at least one photoinitiator.

[0117] It should be understood that any suitable deposition method may be used. Examples of suitable deposition techniques include spray coating, spin coating, dunk or dip coating, puddle dispensing, etc. In one example, the WS material 24 is spin coated onto the imprint apparatus 10, 10'.

[0118] The method further includes curing the WS material 24, thereby forming a fabricated stamp 28 including a negative replica of the plurality of features 14 in contact (e.g., direct physical contact) with the anti-stick layer 16 on the imprint apparatus 10, 10' (FIG. 4C). In one example, the WS material 24 is cured by ultraviolet (UV) radiation. In another example, the WS material 24 is thermally cured. In some examples, the thermal curing may occur at a temperature ranging from about 60° C. to about 300° C.

[0119] As shown in FIG. 4C, the method may further include attaching the backplane 26 to the working stamp 28. In one example, a polymer film including an adhesive material (not shown) may be applied (e.g., by roll coating) to the WS material 24 before curing, such that the adhesive contacts the WS material 24. Thereafter, upon exposure to UV radiation, both the WS material 24 and the adhesive material cure, thereby adhering the working stamp 28 to the backplane 26. It should be understood that the backplane 26 may be formed from any suitable polymer material. In one example, the backplane 26 is a polyethylene terephthalate (PET) film. Other examples of the backplane 26 include poly(vinyl chloride) (PVC) and propylene oxide (PO). In some embodiments, the backplane material is flexible. It should be further understood that the adhesive material may be any suitable UV-curable material.

[0120] 4D, the method further includes peeling the working stamp 28 from the imprint apparatus 10, 10'. In one example, the peeling is by pulling / peeling the hardened working stamp 28 from the imprint apparatus 10, 10'. Other removal techniques may also be used.

[0121] The released working stamp 28 has greater than 99.9% of the features 14 transferred cleanly (due to the lack of film defects in the imprint apparatus 10, 10') and is at least substantially free (i.e., free or substantially free) of the anti-stick layer 16 material. Without being bound by any theory, it is believed that no transfer of anti-stick layer 16 material should occur during the fabrication process of the working stamp 28 because any excess of unreacted monomers, dimers, trimers and / or high molecular weight species should have been washed away prior to fabrication of the anti-stick layer 16. If any of the anti-stick layer 16 material transfers, it is at most at parts per million (ppm) levels, and therefore the working stamp 28 is believed to be substantially free of the anti-stick layer 16 material.

[0122] To further illustrate the present disclosure, examples are presented herein. It should be understood that these examples are presented for illustrative purposes and should not be construed as limiting the scope of the present disclosure. EXAMPLES

[0123] Non-limiting examples

[0124] Example 1 To investigate film defects, formulations were prepared using pure silane material containing both a first silane monomer (having a Si-OCH2CH3 group) and about 5 wt% to about 10 wt% of a second silane monomer (containing at least one Si-Cl group). The formulations were prepared by diluting the pure silane material with anhydrous tetrahydrofuran (THF) to obtain a solution containing about 10 wt% of the first silane monomer. An inhibitor, butylated hydroxytoluene (BHT), was added.

[0125] The formulation was spin coated onto a silicon master having features defined therein. The features had a diameter of about 220 nm and an average pitch of about 350 nm. The spin coating involved first dispensing the formulation at a very low RPM, followed by an incubation of about 30 seconds, followed by a faster spin to remove excess formulation.

[0126] The coated silicon master was exposed to heat at about 130° C. for about 10 minutes, then rinsed with solvent, and then exposed to further heat at about 130° C. for about 2 minutes.

[0127] After processing, images were taken of large areas of the coated silicon master containing multiple features. Figure 5A shows one film defect covering multiple features. The darker area in the center of the film defect indicated that more material had been deposited in that area. The cross-sectional view in Figure 5B shows one of the features covered with a film defect. As shown, the accumulated material that was part of the film defect had completely blocked the feature. From these results, the formation of film defects was detected when the amount of second silane monomer (specifically chloride) in the pure material was 5% or more.

[0128] Example 2 To investigate the effect of chloride content on the formation of film defects, formulations containing different amounts of a second silane monomer were prepared.

[0129] Four different formulations were prepared. Two pure silane materials were used to prepare these formulations. The first pure silane material included a first silane monomer (containing a Si-OCH2CH3 group) and about 0.14 wt% of a second silane monomer (containing at least one Si-Cl group) to provide a total of about 50 ppm of hydrolyzable chloride. The second pure silane material included a first silane monomer (containing a Si-OCH2CH3 group) and about 10 wt% of a second silane monomer (containing at least one Si-Cl group) to provide a total of about 4000 ppm of hydrolyzable chloride. The first and second pure silane materials were combined to produce Pure Material A (about 0.28 wt% second silane monomer) containing about 100 ppm hydrolyzable chloride, Pure Material B (about 0.56 wt% second silane monomer) containing about 200 ppm hydrolyzable chloride, Pure Material C (about 1.4 wt% second silane monomer) containing about 500 ppm hydrolyzable chloride, and Pure Material D (about 2.8 wt% second silane monomer) containing about 1000 ppm hydrolyzable chloride. Once the desired percentages were obtained for each pure material, each pure material was diluted with anhydrous THF to formulate silane solutions (Solution A made with Pure Material A, Solution B made with Pure Material B, Solution C made with Pure Material C, and Solution D made with Pure Material D), each containing about 10 wt% first silane monomer.

[0130] Solutions A-D were coated onto respective silicon masters and cured using the process described in Example 1 to form respective imprint devices A-D.

[0131] A respective fabricated stamp was generated from each of the imprint apparatuses A-D, and a respective imprint A-D was generated using the fabricated stamps A-D. If a film defect is generated in the imprint apparatus, the covered or filled feature will not be transferable to the fabricated stamp or to the final imprint. Any defects (e.g., imprint defects) observed in the imprint are indicative of a film defect present in the imprint apparatus.

[0132] Imprints A to D were imaged using a Keyence confocal microscope, and the images are shown in FIG. 6A (imprint A formed using processed stamp A generated from imprint device A), FIG. 6B (imprint B formed using processed stamp B generated from imprint device B), FIG. 6C (imprint C formed using processed stamp C generated from imprint device C), and FIG. 6D (imprint D formed using processed stamp D generated from imprint device D). As shown in FIG. 6A and FIG. 6B, no imprint defects were observed in imprints A and B generated from processed stamps A and B prepared using imprint devices A and B, respectively, and the anti-sticking layers of imprint devices A and B were prepared using formulations having hydrolyzable chloride levels of 100 ppm and 200 ppm, respectively. These images show that no film defects are present in imprint devices A and B. In imprint C (produced from fabricated stamp C prepared using imprint device C, where the anti-sticking layer of imprint device C was prepared using solution C with a hydrolyzable chloride level of 500 ppm), several imprint defects were observed, as shown by the lighter dots in FIG. 6C. This image shows that several film defects were present in imprint device C. In imprint D (produced from fabricated stamp D prepared using imprint device D, where the anti-sticking layer of imprint device D was prepared using solution D with a hydrolyzable chloride level of 1000 ppm), several more imprint defects were observed, as shown in FIG. 6D. This image shows that several more film defects were present in imprint device D.

[0133] The imprint defects were quantified by an image analysis script. The imprint defects are correlated to film defects in the anti-sticking layer of the imprinted devices formed using solutions A to D. The correlation results reflecting the film defects are shown in Figure 7. Figure 7 shows three sets of quantification results.

[0134] The top graph of FIG. 7 shows the percentage of film defects produced in the anti-stick layer versus the hydrolyzable chloride level (ppm) in the formulation used to produce the anti-stick layer. The percentage is relative to the total number of features in the imprinting device. As shown, the 100 ppm and 200 ppm formulations (Solution A and Solution B, respectively) produced 0% film defects, while a slightly higher percentage was observed for the 500 ppm formulation (Solution C), but even this percentage was less than 0.05%. The 1000 ppm formulation (Solution D) produced more than 0.1% film defects.

[0135] The middle graph in Figure 7 shows the average number of film defects produced in the anti-stick layer versus the hydrolyzable chloride level (ppm) in the formulation used to produce the anti-stick layer. For each data point in the middle graph in Figure 7, an average of eight cross-shaped samples was taken. As shown, the average number of film defects was zero for the 100 ppm and 200 ppm formulations (Solution A and Solution B, respectively). For the 500 ppm formulation (Solution C), the average number of film defects was less than 1.5. Meanwhile, for the 1000 ppm formulation (Solution D), the average number of film defects was more than 10.

[0136] The bottom graph of Figure 7 shows the average pixel count of film defects produced in the anti-stick layer versus the hydrolyzable chloride level (ppm) in the formulation used to produce the anti-stick layer. The 100 ppm and 200 ppm formulations (Solution A and Solution B, respectively) did not produce any film defects, and therefore had an average pixel count of zero. The 500 ppm formulation (Solution C) produced an average pixel count of film defects between 10 and 15. Meanwhile, the 1000 ppm formulation (Solution D) produced an average pixel count of film defects greater than 40.

[0137] These results confirmed that as the content of hydrolyzable chloride in the formulation increased up to 1000 ppm, film defects formed more easily and became larger.

[0138] Example 3 In this example, two formulations were prepared. A formulation was prepared using pure silane material containing both a first silane monomer (containing a Si-OCH2CH3 group) and about 0.14 wt% of a second silane monomer (containing at least one Si-Cl group). The formulation was prepared by diluting the pure silane material with anhydrous tetrahydrofuran (THF) to obtain a solution containing about 10 wt% of the first silane monomer. An inhibitor, butylated hydroxytoluene (BHT), was added.

[0139] The first solution was used to produce imprint device Y within one day of formulation production and therefore was not aged. The unaged formulation was spin coated and cured as described in Example 1. A textured stamp was produced from imprint device Y and 25 imprints were produced using the textured stamp. Images of the 1st, 10th and 25th imprints are shown in Figures 8A, 8B and 8C, respectively. These images show high levels of desorption defects or missing patterned areas at the bottom of the wafer. The missing patterned areas are circled in each of the figures. Such desorption defects are characteristic of poor coating of the formulation onto the silicon master during production of the imprint device.

[0140] The second solution was kept at room temperature storage for 8 days and then used to produce imprint device Z. This formulation was also spin coated and cured as described in Example 1. Another engineered stamp was produced from imprint device Z and 25 imprints were produced using the engineered stamp. Images of the 1st, 10th and 25th imprints are shown in Figures 9A, 9B and 9C, respectively. These images show much lower levels of detachment defects or missing patterned areas at the bottom of the wafer. These results indicate that aging the formulation helped improve the coatability of the formulation when it was produced using a pure silane material with a desirably low level of hydrolyzable chloride in this example.

[0141] Example 4 In this example, a starting pure silane material was used that contained both a first silane monomer (containing a Si-OCH2CH3 group) and about 10 wt% of a second silane monomer (containing at least one Si-Cl group) that resulted in about 4500 ppm of hydrolyzable chloride. The pure silane material was diluted with ethyl acetate. An equal amount of sodium bicarbonate solution was added and the combined solution was mixed for about 10 minutes. The pH of the aqueous phase was confirmed to be about 9 by pH paper. The aqueous phase was then removed. The remaining organic phase was dried over anhydrous magnesium sulfate for about 20 minutes. The anhydrous magnesium sulfate was filtered and any remaining ethyl acetate was removed by rotary evaporation. This resulted in a colorless liquid. The liquid was titrated to determine the percentage of remaining hydrolyzable chloride. This process was repeated until the level of hydrolyzable chloride was about 160 ppm. The solution was diluted with anhydrous THF to formulate a solution containing about 10 wt% of the first silane monomer. The formulation was spin-coated and cured as described in Example 1. No coating defects were observed.

[0142] Additional Notes It should be understood that all combinations of the above concepts and further concepts discussed in more detail below (provided that such concepts are not mutually inconsistent) are contemplated as part of the inventive subject matter disclosed herein. In particular, all combinations of claimed subject matter appearing at the end of this disclosure are contemplated as part of the inventive subject matter disclosed herein. It should also be understood that the terminology expressly used in this specification and which may also appear in any disclosure incorporated by reference should be given the meaning most consistent with the particular concepts disclosed herein.

[0143] References throughout this specification to "one example," "another example," "an example," etc. mean that particular elements (e.g., features, structures and / or characteristics) described in connection with an example are included in at least one example described herein and may or may not be present in other examples. In addition, unless the context clearly dictates otherwise, it should be understood that elements described in connection with any example may be combined in any suitable manner in the various examples.

[0144] Ranges set forth herein should be understood to include the stated ranges and any values ​​or subranges within the stated ranges as if such values ​​or subranges were expressly recited. For example, a range of about 350 nm to about 400 nm should be interpreted to include not only the explicitly recited limits of about 350 nm to about 400 nm, but also individual values ​​such as about 358 nm, about 375.5 nm, and subranges such as about 355 nm to about 395 nm, about 350 nm to about 375 nm, etc. Furthermore, when "about" and / or "substantially" are used to describe values, they are intended to encompass slight variations (up to ±10%) from the stated values.

[0145] While certain examples have been described in detail, it should be understood that the disclosed examples may be modified, and therefore the foregoing description is considered to be non-limiting. [Explanation of symbols]

[0146] 10 Imprinting device 10' Imprinting device 12 Silicon Master 13 Substrate 14 Features 14' Hollow 15 Patterned Area 16 Anti-sticking layer 18 Interstitial area 20 aperture 22 Compounds 24 WS material 26 Backplane 28 Processing Stamp

Claims

1. A silicon master comprising a plurality of features arranged with an average pitch of less than 425 nm, each of the plurality of features comprising a recess having an opening whose maximum opening dimension is less than 300 nm; and an anti-stick layer coating the surface of the silicon master, the anti-stick layer comprising a cross-linked silane polymer network; First silane monomer: 【Chemistry 1】 and 0.28 wt% to 1.1 wt% of a second silane monomer based on the total amount of silane present in the formulation 【Chemistry 2】 An anti-stick layer produced using a formulation comprising An imprint apparatus comprising:

2. 10. The imprint apparatus of claim 1, wherein less than 0.15% of a total number of the plurality of features have film defects.

3. 10. The imprinting apparatus of claim 1, wherein the anti-stick layer coats a wall of each of the recesses without capping the openings or completely filling the recesses.

4. 2. The imprinting apparatus of claim 1, wherein a maximum opening dimension of each of the plurality of features is in the range of 170 nm to 290 nm.

5. 2. The imprinting apparatus of claim 1, wherein a maximum opening dimension of each of the plurality of features is in the range of 200 nm to 250 nm.

6. 2. The imprinting apparatus of claim 1, wherein the average pitch is in the range of 340 nm to 410 nm.

7. 2. The imprinting apparatus of claim 1, wherein the average pitch is in the range of 350 nm to 400 nm.

8. A process for applying a formulation to a surface of a silicon master including a plurality of features arranged with an average pitch of less than 425 nm, each of the plurality of features having a maximum opening dimension that is less than 300 nm, the formulation comprising: First silane monomer: 【Chemistry 3】 and 0.28 wt% to 1.1 wt% of a second silane monomer based on the total amount of silane present in the formulation 【Chemistry 4】 A process comprising: Curing the applied formulation, thereby forming an anti-stick layer.

16. A method for making an imprint apparatus, comprising:

9. The compound is i) a dimer comprising two linking units of a first silane monomer; ii) a trimer comprising three linking units of a first silane monomer; iii) a polymeric species containing more than three linking units of the first silane monomer; iv) a first silane monomer having at least one hydrolyzable ethoxy group; or v) any combination of i), ii), iii) and iv). The method of claim 8, further comprising:

10. The hardening process is Incubation process, A rinsing process after the incubation process; and 9. The method of claim 8, further comprising a heating process after the rinsing process.

11. 11. The method of claim 10, wherein the incubation and heating processes are carried out independently at a temperature ranging from 20° C. to 250° C. for a time ranging from 1 minute to 30 minutes.

12. diluting a pure silane material including first and second silane monomers with a first solvent to form a precursor solution; exposing the precursor solution to a basic aqueous solution to produce an aqueous phase and an organic phase, the organic phase comprising the first solvent; removing the aqueous phase; removing the first solvent from the organic phase to obtain a purified silane material; and Diluting the purified silane material with a second solvent.

9. The method of claim 8, further comprising producing the blend by:

13. 13. The method of claim 12, wherein the second solvent is an aprotic solvent that i) solvates the purified silane material and ii) has a boiling point in the range of 50°C to 250°C.

14. The method of claim 12, wherein the purified silane material is present in the formulation in an amount ranging from 1 wt% to 20 wt%.

15. The method of claim 8 , wherein the step of applying the formulation comprises spin coating.

16. The formulation is solvent-free, or The formulation contains 1 wt% to 20 wt% of a first silane monomer. The method of claim 8, wherein the 17. A process for depositing a formulation on a silicon master including a plurality of features arranged with an average pitch of less than 425 nm, each of the plurality of features having a maximum opening dimension of less than 300 nm, the formulation comprising a first silane monomer, 【Chemistry 5】 and 0.28 wt% to 1.1 wt% of a second silane monomer based on the total amount of silane present in the formulation 【Chemistry 6】 A process comprising: curing the formulation, thereby forming an anti-stick layer on the silicon master; depositing a silicon-based fabrication stamp material onto an anti-stick layer of the imprint device; curing the silicon-based fabrication stamp material to form a fabrication stamp including a negative replica of the plurality of features; and A step of peeling off the processing stamp from the imprint device.

46. ​​A method for making an imprinting stamp comprising forming an imprinting device by

18. Curing the formulation comprises: Incubation process, A rinsing process after the incubation process; and 20. The method of claim 17, further comprising a heating process after the rinsing process.

19. 20. The method of claim 18, wherein the incubation and heating processes are carried out independently at a temperature ranging from 20° C. to 250° C. for a time ranging from 1 minute to 30 minutes.

20. The method of claim 18 , wherein the silicon-based fabrication stamp material comprises a silicon acrylate monomer.

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