Cosmetic composition for preventing, ameliorating, or treating atopy comprising rock salt as active ingredient

Abstract

The present invention relates to a cosmetic composition for preventing, ameliorating, or treating atopy comprising rock salt as an active ingredient and, more particularly, to a cosmetic composition for preventing, ameliorating, or treating atopy comprising rock salt, epidermal growth factor (EGF), and natural extracts as active ingredients.

Description

Cosmetic composition for preventing, improving or treating atopic dermatitis containing rock salt as an active ingredient

[0001] The present invention relates to a cosmetic composition for preventing, improving or treating atopic dermatitis, comprising rock salt as an active ingredient, and more specifically, to a cosmetic composition for preventing, improving or treating atopic dermatitis, comprising rock salt, epidermal growth factor (EGF) and a natural extract as active ingredients.

[0002] Atopic dermatitis is a long-term form of skin inflammation and is a type of autoimmune disease. When atopic dermatitis develops, the skin becomes dry and scaly, and red, itchy patches form on the arms, legs, face, and neck. It typically begins in childhood and can vary in severity over the years.

[0003] The cause of atopic dermatitis is not clearly known, but it is believed to be mainly due to an imbalance in immune function, and it is a chronic inflammatory skin disease that is caused by a complex interaction of environmental, social, psychological, and genetic factors.

[0004] The number of patients with atopic dermatitis is steadily increasing, and the treatment methods for atopic dermatitis include drug therapy, methods of supplying moisture to the skin with moisturizers, methods of alleviating atopic dermatitis by removing inhaled allergens such as house dust mites, pollen, and cockroaches, methods of avoiding major food allergens such as milk, eggs, and peanuts, and emotional stability, and in severe cases of atopic dermatitis, treatments such as ultraviolet ray therapy, steroids, immunosuppressants, interferon, and immune globulin are also used.

[0005] However, long-term use or abuse of medications such as steroids, antihistamines, immunosuppressants, interferons, and immunoglobulins can lead to developmental delays in children and diabetes and high blood pressure in adults. In particular, antibiotics are becoming increasingly resistant to many pathogens due to their misuse and their lifespans are shortening. Therefore, there is a growing need for natural compositions that are effective in preventing, improving, or treating atopic dermatitis, while also being safe and free of side effects.

[0006] Meanwhile, rock salt, also known as stone salt or rock salt, is a naturally occurring sodium chloride crystal formed when seawater evaporates, leaving behind a mineral called salt. While mostly colorless and transparent, it can also be yellow, red, or blue. It is used as a raw material for industrial salt, table salt, and soda. While shampoo compositions, rock salt beds, and aroma rock salt lamps containing rock salt have been disclosed, a cosmetic composition containing rock salt for preventing, improving, or treating atopic dermatitis, such as the present invention, has not been disclosed.

[0007] The purpose of the present invention is to provide a cosmetic composition for preventing, improving or treating atopic dermatitis, which comprises rock salt, epidermal growth factor (EGF) and natural extract as active ingredients.

[0008] The technical problems to be solved by the invention are not limited to the technical problems mentioned above, and other technical problems not mentioned can be clearly understood by a person having ordinary skill in the art from the description of the present invention.

[0009] The present invention provides a cosmetic composition for preventing, improving or treating atopic dermatitis, characterized in that it comprises rock salt, epidermal growth factor (EGF) and a natural extract as active ingredients.

[0010] In the present invention, the cosmetic composition is characterized in that it comprises 0.1 to 2 parts by weight of rock salt; 2 to 8 parts by weight of epidermal growth factor (EGF); and 90 to 98 parts by weight of a natural extract, based on 100 parts by weight of the cosmetic composition.

[0011] In the present invention, the natural extract is characterized in that it includes at least one selected from the group consisting of ginseng, propolis, gastronomy, green tea, angelica, mimosa, Houttuynia cordata, and white paper.

[0012] In the present invention, the cosmetic composition is characterized in that it is in the form of a toner, toner, essence, cream, pack, gel, lotion, ointment, patch, foam, spray, powder, soap, bath agent, or spray agent.

[0013] In the present invention, the natural extract is characterized in that one or more samples selected from the group consisting of Sophora japonica, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji are extracted with hot water at 80 to 110°C for 2 to 6 hours, the hot water-extracted crude extract is filtered under reduced pressure, and the filtrate is concentrated in a water bath at 30 to 50°C.

[0014] The cosmetic composition for preventing, improving or treating atopic dermatitis, comprising the rock salt of the present invention, epidermal growth factor (EGF) and natural extract as active ingredients, has skin moisturizing, anti-inflammatory, antioxidant and atopic dermatitis improving effects.

[0015] The cosmetic composition for preventing, improving or treating atopic dermatitis comprising the rock salt, epidermal growth factor (EGF) and natural extract of the present invention as active ingredients has an excellent atopic dermatitis treatment effect and is highly safe, so it can be used as a natural atopic dermatitis treatment cosmetic that does not cause side effects on the skin.

[0016] The effects of the present invention are not limited to the effects mentioned above, and other effects not mentioned will be clearly understood by those skilled in the art from the description of the claims.

[0017] Figure 1 is a graph showing the anti-inflammatory effect of a cosmetic composition for preventing, improving or treating atopic dermatitis, including rock salt, epidermal growth factor (EGF) and natural extracts, as measured by the inhibition ability of nitric oxide (NO), an intracellular inflammatory substance.

[0018] Figure 2 is a graph showing the antioxidant effect of a cosmetic composition for preventing, improving or treating atopic dermatitis containing rock salt, epidermal growth factor (EGF) and natural extracts, as measured by DPPH assay.

[0019] The terms used in this specification have been selected from widely used, current terms, taking into account the functions of the present invention. However, these terms may vary depending on the intentions of those skilled in the art, precedents, the emergence of new technologies, etc. Furthermore, in certain cases, terms may be arbitrarily selected by the applicant, and in such cases, their meanings will be described in detail in the relevant description of the invention. Therefore, the terms used in this invention should not be defined simply as names, but rather based on their inherent meanings and the overall content of the present invention.

[0020] Unless otherwise defined, all terms used herein, including technical or scientific terms, have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. Terms defined in commonly used dictionaries should be interpreted as having a meaning consistent with their meaning in the context of the relevant technology, and shall not be interpreted in an idealized or overly formal sense unless explicitly defined herein.

[0021] Numerical ranges are inclusive of the numbers defined in the ranges above. Every maximum numerical limitation given throughout this specification includes every lower numerical limitation, as if that lower numerical limitation were explicitly stated. Every minimum numerical limitation given throughout this specification includes every higher numerical limitation, as if that higher numerical limitation were explicitly stated. Every numerical limitation given throughout this specification will include every better numerical range within that broader numerical range, as if that narrower numerical limitation were explicitly stated.

[0022]

[0023] Hereinafter, the present invention will be described in detail.

[0024]

[0025] The present invention provides a cosmetic composition for preventing, improving or treating atopic dermatitis, comprising rock salt, epidermal growth factor (EGF) and a natural extract as active ingredients.

[0026] In the present invention, the cosmetic composition can be manufactured using 0.5 to 2 kg of each of at least one sample selected from the group consisting of Sophora ginseng, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji, and the Sophora ginseng, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji can be used for hot water extraction in a dried state as needed, and the drying method may include, but is not limited to, hot air drying, freeze drying, spray drying, vacuum drying, or foam drying. The crude extract can be obtained by hot water extraction of at least one sample selected from the group consisting of Sophora ginseng, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji at 80 to 110°C for 2 to 6 hours, and preferably, the crude extract can be obtained by hot water extraction at 90 to 105°C for 3 to 5 hours. The above hot water-extracted crude extract can be filtered to obtain a filtrate. The filtration method includes atmospheric pressure filtration, reduced pressure filtration, and pressure filtration, but preferably, a reduced pressure filtration method using a reduced pressure suction filter can be used. The filtrate can be concentrated in a water bath at 30 to 50°C, and preferably, it can be concentrated using a vacuum rotary evaporator in a water bath at 35 to 45°C.

[0027] In the present invention, the rock salt may be Andean Uyuni rock salt, Himalayan rock salt, Himalayan pink salt rock salt, or a combination thereof, and preferably Andean Uyuni rock salt may be used. The particle size of the rock salt is not particularly limited, but may be 50 to 150 mesh, and preferably 70 to 120 mesh.

[0028] In addition, in the present invention, the cosmetic composition can be prepared as an aqueous composition by adding distilled water to a mixture containing 0.1 to 2 parts by weight of rock salt; 2 to 8 parts by weight of epidermal growth factor (EGF); and 90 to 98 parts by weight of a natural extract, based on 100 parts by weight of the cosmetic composition, and preferably, the cosmetic composition can be prepared as an aqueous composition by adding distilled water to a mixture containing 1 part by weight of rock salt, 5 parts by weight of epidermal growth factor (EGF), and 94 parts by weight of a natural extract including Sophora ginseng, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji, based on 100 parts by weight of the cosmetic composition.

[0029] In addition, the cosmetic composition may be manufactured in the form of, but is not limited to, a lotion, a toner, an essence, a cream, a pack, a gel, a lotion, an ointment, a patch, a foam, a spray, a powder, a soap, a bath additive, a spray, a nutritional lotion, a nutritional essence, a massage cream, a cosmetic bath additive, a body lotion, a body milk, a bath oil, a baby oil, a baby powder, a shower gel, a shower cream, a sunscreen lotion, a sunscreen cream, a suntan cream, a UV-blocking cosmetic, a cleansing milk, a skin whitening cream, a hand lotion, a hair lotion, a cosmetic cream, a bath soap, a liquid soap, a beauty soap, a shampoo, a hand cleanser, a cream soap, a facial wash, a hair rinse, a cosmetic soap, a tooth whitening gel or toothpaste, etc.

[0030] Hereinafter, examples of the present invention will be described in detail, but it is obvious that the present invention is not limited to the following examples.

[0031]

[0032] Example 1. Preparation of a composition for preventing, improving, or treating atopic dermatitis comprising rock salt, epidermal growth factor (EGF), and natural extracts.

[0033] 1 kg each of Sophora japonica, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji were extracted with hot water at 100℃ for 4 hours. The crude extract extracted with hot water was filtered under reduced pressure using a vacuum suction filter (Whatman NO. 1002-185), and the filtrate was concentrated using a vacuum rotary evaporator in a 40℃ water bath to prepare a natural extract including Sophora japonica, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji. Rock salt was prepared by grinding Andean Uyuni rock salt using a grinder to produce rock salt fine powder, and the particle size of the rock salt was prepared to be 100 mesh. Epidermal growth factor (EGF) was purchased and used as a commercial product (100 ppm).

[0034] Based on 100 parts by weight of the above cosmetic composition, 1 part by weight of rock salt, 5 parts by weight of epidermal growth factor (EGF), and 94 parts by weight of natural extracts including Sophora japonica, propolis, Gastrodia elata, green tea, Angelica gigas, mimosa, Houttuynia cordata, and Baekji were mixed with 1 g of distilled water to prepare a composition for preventing, improving, or treating atopic dermatitis in an aqueous solution.

[0035]

[0036] Comparative Example 1. Preparation of a composition for preventing, improving, or treating atopic dermatitis containing rock salt and not containing epidermal growth factor (EGF) and natural extracts.

[0037] Rock salt was prepared in the same manner as in Example 1, and 1 g of a mixture of 1 part by weight of rock salt and 99 parts by weight of distilled water based on 100 parts by weight of the cosmetic composition was mixed with 9 g of distilled water to prepare a composition for preventing, improving, or treating atopic dermatitis in an aqueous solution.

[0038]

[0039] Comparative Example 2. Preparation of a composition for preventing, improving, or treating atopic dermatitis containing a natural extract and not containing epidermal growth factor (EGF) and rock salt.

[0040] A natural extract was prepared in the same manner as in Example 1, and 1 g of the natural extract was mixed with 9 g of distilled water to prepare an aqueous composition.

[0041]

[0042] Experimental Example 1. Moisturizing effect of a composition for preventing, improving, or treating atopic dermatitis according to the present invention

[0043] In order to evaluate the skin moisturizing effect of the composition for preventing, improving or treating atopic dermatitis manufactured in the above Example 1, 5 male and 5 female patients with atopic dermatitis were recruited. The ages of the 10 atopic dermatitis patients were 15 to 32 years old, and the average age was 26 years old. The composition according to the above Example 1 was applied to the skin of the 10 atopic dermatitis patients, and the moisture content was measured. The moisture content was measured by the skin capacitance measurement method, and the device used was a skin moisture meter (Corneometer CM 825).

[0044] The 10 patients with atopic dermatitis were kept under constant temperature and humidity conditions of 20 to 22°C and 40 to 55% relative humidity for 30 minutes before conducting Experimental Example 1. The composition according to Example 1 was uniformly applied to the right upper arms of the 10 patients with atopic dermatitis who had been kept under constant temperature and humidity conditions for 30 minutes, and the skin moisture content was measured over time after application. The left upper arm, to which the sample was not applied, was set as a control, and the skin moisture content was measured over time after application in the same manner as the right upper arm. In addition, the composition not containing the epidermal growth factor and natural extract prepared in Comparative Example 1 was also measured for skin moisture content over time after application in the same manner as the composition prepared in Example 1. The results of Experimental Example 1 are shown in Table 1.

[0045]

[0046]

[0047]

[0048] As a result of Experimental Example 1, there was almost no change in the skin moisture content in the control group, and the skin moisture content was 65 points 2 hours after applying the composition for preventing, improving, or treating atopic dermatitis prepared by the method of Example 1, and 31 points after 24 hours, confirming that the skin moisturizing effect was excellent. On the other hand, the skin moisture content was 10 points 2 hours after applying the composition prepared by the method of Comparative Example 1, and 1 point after 24 hours, confirming that the skin moisturizing effect of the composition according to Example 1 was superior to that of the composition according to Comparative Example 1. Therefore, in the composition for preventing, improving, or treating atopic dermatitis according to the present invention, epidermal growth factor (EGF) and natural extracts are effective in the skin moisturizing effect.

[0049]

[0050] Experimental Example 2. Anti-inflammatory effect of a composition for preventing, improving, or treating atopic dermatitis according to the present invention.

[0051] To confirm the anti-inflammatory effect of the composition for preventing, improving, or treating atopic dermatitis according to the present invention, the amount of nitric oxide (NO) production was measured. The composition for preventing, improving, or treating atopic dermatitis was prepared in the same manner as in Example 1. RAW264.7 cells, a type of macrophage cell line, were seeded in a 96-well plate at a density of 5 × 10 5Cells were seeded so that they were 10 cells / well, and when the confluency of the cells was 80%, the cells were washed twice with PBS and then cultured for 18 hours using serum-free medium. The cultured cells were treated with the composition according to Example 1 at concentrations of 0, 0.1, 1, 5, 10, or 30% (v / v) and cultured for 1 hour, and then treated with 1 μg / mL of Lipopolysacchride (LPS) and cultured for 24 hours. The supernatant of the cells treated with the LPS and cultured was reacted with Griess reagent, and the absorbance was measured at 540 nm with an ELISA reader to calculate the amount of NO production as a percentage, and the percentage is shown in a graph in Fig. 1.

[0052] As a result of this experimental example 2, the amount of NO production decreased as the composition according to Example 1 was treated at different concentrations, and it was confirmed that the composition according to Example 1 significantly inhibited the production of NO, an inflammatory substance, in RAW264.7 cells stimulated with LPS.

[0053]

[0054] Experimental Example 3. Antioxidant effect of a composition for preventing, improving, or treating atopic dermatitis according to the present invention.

[0055] To determine the antioxidant effect of the composition for preventing, improving, or treating atopic dermatitis according to the present invention, a DPPH assay was performed. The DPPH assay is an experimental method for evaluating the radical scavenging ability of a sample. It measures the reducing power of a sample through its electron-donating capacity to DPPH, a relatively stable radical.

[0056] A composition for preventing, improving, or treating atopic dermatitis was prepared in the same manner as in Example 1. 1 mL of ethanol was added to 1 mL of a 0.2 mM DPPH solution dissolved in methanol, 1 mL of the composition according to Example 1 was added, and the mixture was mixed, then allowed to stand at 25°C for 10 minutes, and the absorbance was measured at 517 nm using a spectrophotometer. In addition, a sample in which the composition according to Example 1 was not added to the 0.2 mM DPPH solution dissolved in methanol was measured for absorbance in the same manner to set a negative control group, and the absorbance of α-Tocopherol was measured to set a positive control group. The results of Experimental Example 3 are shown graphically in Fig. 2, and the free radical scavenging activity is indicated on the vertical axis of Fig. 2, which means the concentration of the sample required to reduce the concentration of DPPH by 50%. The formula used to calculate the above free radical scavenging activity is described in Formula 1 below.

[0057]

[0058] [Formula 1]

[0059]

[0060]

[0061] (In the above equation 1, A Experimen t means the absorbance of a sample containing the composition according to the above Example 1, and A Control refers to the absorbance of the negative control group, and A Blank refers to the absorbance of distilled water.)

[0062]

[0063] As a result of this experimental example 3, it was confirmed that the composition for preventing, improving or treating atopic dermatitis according to the above example 1 has superior free radical scavenging activity compared to the negative control group.

[0064]

[0065] Experimental Example 4. Effect of the composition for preventing, improving, or treating atopic dermatitis according to the present invention on improving atopic dermatitis.

[0066] In order to confirm the atopic dermatitis improving effect of the composition for preventing, improving or treating atopic dermatitis according to the present invention, Experimental Example 4 was conducted. A composition for preventing, improving or treating atopic dermatitis containing all of rock salt, epidermal growth factor (EGF) and natural extracts was prepared in the same manner as Example 1, and a composition containing only natural extracts extracted in the same manner as Comparative Example 2 was prepared. The composition according to Example 1 or Comparative Example 2 was applied to 10 atopic dermatitis patients for 30 days, and then the improvement in symptoms such as relief of itching, improvement of erythema and improvement of affected area was confirmed. The results according to Experimental Example 4 are shown in Table 2.

[0067]

[0068]

[0069]

[0070] As a result of Experimental Example 4, when 10 atopic patients applied the composition according to Comparative Example 2, itching was relieved in 7 patients, erythema was improved in 2 patients, and the affected area was improved in 2 patients. On the other hand, when 10 atopic patients applied the composition according to Example 1, itching was relieved in 9 patients, erythema was improved in 3 patients, and the affected area was improved in 3 patients. Therefore, it was confirmed that the composition containing all of the above rock salt, epidermal growth factor (EGF), and natural extracts was more effective in improving atopic dermatitis than the composition containing only the natural extracts.

[0071]

[0072] From the above description, those skilled in the art will understand that the present invention can be implemented in other specific forms without altering its technical spirit or essential features. In this regard, it should be understood that the embodiments described above are illustrative in all respects and are not limiting.

Claims

1. A cosmetic composition for preventing, improving or treating atopic dermatitis, characterized in that it contains rock salt, epidermal growth factor (EGF) and natural extract as active ingredients.

2. In paragraph 1, The above cosmetic composition Based on 100 parts by weight of cosmetic composition 0.1 to 2 parts by weight of rock salt; 2 to 8 parts by weight of epidermal growth factor (EGF); and A cosmetic composition for preventing, improving or treating atopic dermatitis, characterized in that it comprises 90 to 98 parts by weight of a natural extract.

3. In any one of paragraphs 1 and 2, The above natural extracts are A cosmetic composition for preventing, improving or treating atopic dermatitis, characterized in that it comprises at least one selected from the group consisting of ginseng, propolis, gastrodia elata, green tea, angelica, mimosa, houttuynia cordata and white paper.

4. In paragraph 1, The above cosmetic composition A cosmetic composition for preventing, improving or treating atopic dermatitis, characterized in that it is in the form of a toner, essence, cream, pack, gel, lotion, ointment, patch, foam, spray, powder, soap, bath agent or atomizer.

5. In paragraph 1, The above natural extracts A cosmetic composition for preventing, improving or treating atopic dermatitis, characterized in that one or more samples selected from the group consisting of ginseng, propolis, Gastrodia elata, green tea, angelica, mimosa, Houttuynia cordata and Baekji are hot-extracted at 80 to 110°C for 2 to 6 hours, the hot-extracted crude extract is filtered under reduced pressure, and the filtrate is concentrated in a water bath at 30 to 50°C.

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