A dental varnish for prevention of dental caries formation and for treatment of initial caries, suitable for use in the field of dental technology

Abstract

The invention is related to a fluoride-free dental varnish mainly composed of combination of natural products, belonging to the technical field of dentistry and providing prevention of dental caries and treatment of initial dental caries in preventive applications, supporting surface and subsurface remineralization, and the in vitro activity of this dental varnish.

Description

[0001] A DENTAL VARNISH FOR PREVENTION OF DENTAL CARIES FORMATION AND FOR TREATMENT OF INITIAL CARIES, SUITABLE FOR USE IN THE FIELD OF DENTAL TECHNOLOGY

[0002] TECHNICAL FIELD

[0003] The invention is related to a dental varnish, which is suitable for use in the field of dental technology, for prevention of dental caries, treatment of initial caries with its surface and subsurface remineralization activity.

[0004] In another aspect, the invention is related to a method for the production of the dental varnish of the invention.

[0005] In another aspect, the invention is related to the determination of the in-vitro activity of the dental varnish of the invention.

[0006] PRIOR ART

[0007] Dental caries, which affects the majority of the public, is recognized as a significant health problem by the World Health Organization (WHO). Although dental caries is a preventable disease, it continues to be one of the most common chronic diseases today.

[0008] The World Health Organization (WHO) and the International Association for Dental Research (IADR) are striving to develop the necessary strategies to ensure early diagnosis, preventive practices and effective management of dental caries in order to reduce the impact of dental caries in public. Today, it is known that new approaches are necessary for treating dental caries, which is still quite common worldwide and considered as a global burden. Dental caries is not only a risk factor for oral health, but also carries a significant risk for general health with a direct impact on the life quality. In addition to health problems, dental caries has serious negative effects on well-being of the public at a social and an economic level. For this reason, it is of great importance to intervene before dental caries occurs or when it is still at the initial stage. Dental caries is a multifactorial and dynamic disease resulting from the cycles of de- and remineralization of dental hard tissue. Whether demineralization or remineralization predominates the dental environment, is mainly determined by the balance between the pathological and the protective factors within this ongoing cycle. While protective factors promote remineralization and can arrest caries lesions, pathological factors are expected to shift the balance to the direction of dental caries and disease progression. In the process of caries progression, it is reported that if the caries is still on an initial stage and only limited with dental enamel, this process can be reversed before the formation of cavity occurs.

[0009] Subsurface lesions occur when the amount of the mineral loss on the subsurface level is higher than on the surface, resulting from the ongoing demineralization process with continuous acid attacks and decrease of the oral pH. If enough mineral loss subsequently occurs, the lesion appears clinically as a 'white spot'. In this clinically significant phase of caries development, the lesion can be arrested and reversed. At this point, however, it should be noted that the repair process of the lesion is typically mostly limited to the surface area. Therefore, the subject varnishes of this invention, aim a remineralization effect for both surface and subsurface lesions.

[0010] Initial enamel caries (white spot lesions) are frequently seen in the high risk group of people for dental caries (people with poor oral hygiene, sugar-containing dietary habits, systemic diseases, or people who are receiving medicine therapy, which effects the flow rate of saliva, and orthodontic therapy, etc.).

[0011] Protective and preventive treatments are mainly important in the process of arresting and reversing caries lesions. It is mentioned that in dentistry, preventive practices for patients should start at an earliest age as possible. Primary preventive care includes maintaining oral hygiene, fluoride use, control of sugar intake and a proper diet, as well as education of children and families about these issues. In addition, probiotic therapies, use of polyols and topical agents for providing remineralization are currently amongst the frequently used preventive methods. An early management of dental caries, not only provides the remineralization of non-cavitated lesion, but also is an easier procedure for children than restoration or a further dental treatment. In addition, from an economic point of view, preventive treatments are stated as the cheapest, most effective and best method. The discovery of fluoride as a preventive agent for dental caries and the worldwide fluoride-based caries prevention programs have been cited as the biggest invention for the decline in caries prevalence in developed countries in the second half of the 20th century. Nevertheless, it is reported that untreated caries in permanent dentition is still considered as the most common disease globally. Therefore new caries prevention methods are reported to be necessary to reduce the caries of primary dentition, especially in early childhood; since studies indicate that the prevalence of early childhood caries is on an incline.

[0012] Many clinical trials and in vitro studies published in previous years, have reported the anti-caries activity of fluoride, which is able to regulate the balance between de- and remineralization properly. Fluoride is reported as the cornerstone of the non-invasive treatment of non-cavitated caries lesions; however, its ability to promote remineralization is limited by the available calcium and phosphate ions in the oral environment. Some of the disadvantages of topical fluorides have been addressed by various studies. For example, it has been reported that fluoride predominantly promotes surface remineralization, which can cause hypermineralization with the risk of arresting the developing lesion inside on the subsurface level. An ideal remineralization agent should also perform a subsurface recovery. In addition, topical fluorides have been proven in various studies to be toxic on oral mucosa and gingival cells. Furthermore, Anderson et al. (2016), reported that fluoride varnish application is not providing an advantage for reducing the prevalence of early childhood caries in preschool children, who are living in certain regions with high caries risk. Although fluoride is a highly effective and economical agent in preventing dental caries, it is emphasized that in many cases use of fluoride alone, is not sufficient. Based on the current understanding of the caries process, several ecological strategies for caries prevention, which are suggesting that in the future caries prevention will not focus on fluoride therapies anymore, have already been developed or are currently under research.

[0013] Similarly, the development of novel fluoride-free enamel remineralization systems has accelerated significantly in recent years, while some are under development and the majority in clinical use. Fluoride-free remineralization systems can significantly improve the properties of the tooth such as structure, aesthetics and acid resistance of the remineralized lesion. Those agents currently include substances such as casein phosphopeptide amorphous calcium phosphate (CPP-ACP), amorphous calcium phosphate (ACP), bioactive glass, nano-hydroxyapatite, calcium carbonate carrier (SensiStat), xylitol, P11 -4 and other biomimetic peptide systems, beta tricalcium phosphate, calcium sodium phosphosilicate, amorphous calcium, and polyphosphate products. In addition, there is an increasing interest in the use of biologically active components produced from natural products with therapeutic potential in dentistry. Researchers choose different foods and beverages such as tea, coffee, grapes, propolis or traditional herbs to evaluate these potentials.

[0014] Preventive practices are needed to prevent dental caries from an initial stage and to provide the recovery of caries at this stage. The main preventive practices are oral hygiene, fluoride application, diet regulation, and public education on this subject.

[0015] Moreover, topical applications such as dental varnishes and dental gels are amongst the protective applications that dentists perform professionally. Thanks to these professional applications, a more effective treatment can be provided as the need for patient cooperation is eliminated. These professional applications are mainly preferred for individuals with high caries risk and should be repeated at intervals of 3-6 months depending on the risk.

[0016] Due to their components and structure, dental varnishes cover tooth surfaces with a thin layer formation and thus provide a long-term contact between tooth and active ingredients responsible for remineralization. In this way, dental varnishes ensure a longer and more effective remineralization treatment, making the teeth resistant to caries, as well as remineralizing the caries without cavitation. Although fluoride is considered as a gold standard for dental remineralization, opposing studies are causing scientific controversy and increasing interest in natural products.

[0017] For the relevant technical field, in the study of this invention, it has been determined that those dental health related problems must be eliminated and fluoride-free agents with natural components should be used.

[0018] SUMMARY OF THE INVENTION The present invention relates to a dental varnish for eliminating the above-mentioned disadvantages and bringing new advantages to the relevant technical field.

[0019] The main object of the invention is to introduce a fluoride-free dental varnish with a natural agent as the active substance for preventive and therapeutic dentistry applications.

[0020] Another object of the invention is to introduce a dental varnish providing the prevention of dental caries formation and the treatment of initial caries, due to the widespread prevalence of dental caries.

[0021] A further object of the invention is to introduce a dental varnish which is capable of being an alternative to fluoride-containing dental varnishes, and provides maximum contact time with the tooth surfaces.

[0022] In another aspect, the invention is related to a method for the production of the characterized dental varnish.

[0023] BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 shows the viability percentage of L929 cells (cell viability %) exposed to varnish formulations containing grape seed extract by direct contact for 24 hours, in comparison with negative and positive controls. In this figure, the expressions are; M: Negative control (PBS), PC: 0.4 mg / mL SDS.

[0025] DETAILED DESCRIPTION OF THE INVENTION

[0026] In this detailed description, the subject of the invention relates to a dental varnish and is described only with non-limiting examples for a better understanding of the subject.

[0027] In this invention, "dental varnish" refers to a material used in the field of dentistry that helps prevent and stop the progression of dental caries or recovers the dental caries without cavitation. In the invention, "dental caries" refers to the loss of dental substance due to acidic products formed by bacteria that accumulates to areas on tooth surfaces which cannot be cleaned from food and beverage residues within time. Dental caries can often occur under conditions of poor oral hygiene, frequent sugar intake and lack of the preventive practices.

[0028] In the invention, "grape seed extract" refers to a substance produced from grape seeds.

[0029] In the invention, "xylitol" in dentistry refers to a sugar alcohol, which is a polyol derived from sugar, of which the preventive and therapeutic properties against dental caries have already been proven in many in vitro studies.

[0030] In the invention, "xanthan gum" refers to a polysaccharide with many industrial uses, including its common application as a food additive.

[0031] In the invention, "remineralization" refers to the regaining the minerals lost from the tooth surface during the demineralization process onto the tooth surface.

[0032] In the art, fluoride is considered as a cornerstone of non-invasive treatment of caries without cavitation. However, fluoride has been found to predominantly cause surface remineralization and poses a risk of causing surface hypermineralization and therefore arresting the subsurface lesion inside. The dental varnish of the invention does not contain fluoride. However, even if it is a fluoride-free product, along with the components it contains, the subject tooth varnish provides a remineralization effect which is effective as fluoride. Accordingly, the dental varnish contains at least one plant-based extract.

[0033] The dental varnish of the invention contains the extract of grape seeds as a plant-based extract. Herein, by extract it is meant that the dental varnish contains an extract obtained from the extraction of grape seeds as a component. The dental varnish of the invention contains the grape seed extract in order to provide technical advantage in dental applications. Grape seed extract has been the subject of many studies for its properties of reducing acid production by Mutans Streptococci as well as remineralizing dental tissues. The remineralization of grape seed extract is associated with root caries, dentin and tooth enamel, and the main remineralization mechanism is explained by its function as a collagen cross-linker. With these benefits, it has been determined that the dental varnish with grape seed extract can be an alternative technical solution to fluoride-containing dental varnishes in preventing initial caries and remineralizing tooth enamel.

[0034] The dental varnish of the invention contains grape seed extract at a value in the range of 10% to 20% by weight. Grape seed extract above the specified value ranges can cause clumping and precipitation of tooth varnish. Grape seed extract with specified value ranges, on the other hand, has been determined to have technical properties such as appropriate remineralization effect for tooth varnish, providing the targeted homogeneous appearance, and providing appropriate cytotoxicity.

[0035] The dental varnish of the invention contains grape seed extract as a component. It may also contain at least one natural sugar alcohol as a component. The dental varnish of the invention can contribute to the remineralization property by synergizing the natural sugar alcohol component it contains with the grape seed extract. In addition, dental varnish containing the natural sugar alcohol component together with grape seed extract provides positive side effects in the recovery of surface microhardness, prevention of ion precipitation and effect against microorganisms.

[0036] In a preferred embodiment of the invention, dental varnish may contain grape seed extract at a value in the range of 10% to 20% by weight and at least one sugar alcohol derived from sugar at a value between 10% to 20% by weight.

[0037] The dental varnish of the present invention may contain one of the xylitol and / or erythritol compounds as a natural sugar alcohol. These sugar alcohols are preferred in this invention because they are known for their properties of disrupting energy production processes of Mutans Streptococci, which leads the cell death, reducing the plaque formation and bacterial adhesion to tooth surfaces, and reducing bacterial acid production. Thus, they are able to inhibit enamel demineralization.

[0038] The dental varnish of the invention may contain xylitol as a component at a value in the range of 5% to 25% by weight. In a preferred embodiment, dental varnish contains xylitol at a value in the range of 10% to 20% by weight. The dental varnish of the present invention contains at least one thickening agent or thickener component. In the art, the functions of thickening agent or thickener components are well-known. This invention contains at least one of the groups of gellan gum, pectin, resins / rosins, xanthan gum as a thickening agent or thickener component. In the most preferred embodiment of the invention, dental varnish contains xanthan gum as a thickening agent or thickener component. In the invention, the thickening agent or thickener component is present in the dental varnish at a value between 1% to 10% by weight. The ratios by weight specified here are important because the final product dental varnish is in certain forms in order to achieve the targeted effects in dental applications.

[0039] The dental varnish of the invention contains at least one preservative and solvent agent. In this invention, the preservative and solvent agent is used to ensure that the grape seed extract, used as the main component in dental varnish, dissolve homogeneously and remains stable afterwards. In a preferred embodiment, dental varnish contains an alcohol-based compound as a preservative and solvent agent. In a preferred embodiment, dental varnish contains ethanol as a preservative and solvent agent.

[0040] The dental varnish of the invention contains at least one solvent agent. The solvent agent is added to allow the components to be combined and mixed homogeneously during the production of dental varnish. Preferably, distilled water is used as a solvent.

[0041] The dental varnish of the invention has a pH value in the range of 4 to 7 in order to see the targeted effects suitable for the tooth structure.

[0042] It is aimed to utilize the therapeutic properties of the components at the highest efficiency by ensuring maximum contact time of the dental varnish of the invention along with the components it contains with the tooth. In particular, it is known that the grape seed extract component it contains, increases the remineralization activity in direct correlation to the increase in duration of contact with the teeth. For this reason, the product containing the components being in varnish form also has technical advantages over other forms according to the findings of the inventors. This is because dental varnish allows for a longer contact with the tooth than other forms. In this way, the components can remain stable in the dental varnish at higher concentrations, making them easier to use clinically and their activity longer lasting. The dental varnish of the invention may contain at least one of the compounds of green tea or theobromine as a component to increase the remineralization effect together with the components it contains.

[0043] Examples that show the working principle of the invention and provide detailed information about the invention are given below. Examples are given to provide a better understanding of the invention, and the scope of the invention is not limited to these examples in any manner.

[0044] Examples

[0045] In this section, examples related to production of the sample dental varnish and testing of the biocompatibility values of the obtained dental varnish are presented. The values given in the examples do not limit the scope of protection of the invention.

[0046] Example 1 : Preparation of a dental varnish containing 20% Grape Seed Extract by weight

[0047] The production of grape seed extract is achieved by the extraction method. 200 g of ground black grape seeds are decanted 2 times with n-hexane at a value of 1 -3 liters at room temperature for 10 to 20 minutes for the removal of fixed oils. Then, the remained plant powder is macerated with 2 liters of 80% ethanol (EtOH) at room temperature for 12 hours and extraction process is performed at 45 °C for 3 hours after maceration. After filtration, the filtrate is condensed in a rotary evaporator (rotavapor) and frozen at -80 °C and then lyophilized for 70 hours. As a result of the process, 1 1.57 g (yield: 5.78%) extract is obtained and 5 g of the lyophilized extract is weighed and separated for use in the study. Grape seed extract obtained from the seeds of grapes grown in Turkey is stored in the refrigerator until the production of varnish.

[0048] Example 2: Producing Varnish from Grape Seed Extract

[0049] Example 2a: Production of Dental Varnish Containing 20% Grape Seed Extract

[0050] 1 gram of grape seed extract is weighed and added to 4 mL of 80% ethanol and mixed on a magnetic stirrer without being heated. 0.1 grams (2%) of xanthan gum is dissolved in 1 mL of distilled water, this solution is added to the grape seed extract mixture and the final volume of 5 mL is completed with distilled water. The solution is placed on a magnetic stirrer and mixed further until it becomes homogeneous.

[0051] Example 2b: Production of Dental Varnish Containing 10% Xylitol + 10% Grape Seed Extract

[0052] After 0.5 grams of xylitol is dissolved in 1 .5 mL of distilled water with the magnetic stirrer, 0.1 grams (2%) of xanthan gum is added into it. 0.5 grams of grape seed extract is dissolved in 3.5 mL of 80% ethanol and placed in an ultrasonic bath for homogenization. Due to the degradation of grape seed extract at higher temperatures, the ultrasonic bath is operated at certain time intervals to prevent the mixture against a possible damage caused by the vibrational heat. After all the processes, the xylitol mixture is combined with the grape seed extract mixture, and the final volume of 5 mL is completed with distilled water. The solution placed on a magnetic stirrer until homogenized.

[0053] Example 2c: Production of Dental Varnish Containing 20% Xylitol + 20% Grape Seed Extract

[0054] After 1 grams of xylitol is dissolved in 1 mL of distilled water with a magnetic stirrer, 0.1 grams (2%) of xanthan gum is added into it. Meanwhile, 1 grams of grape seed extract is dissolved in 4 mL of 80% ethanol and placed in an ultrasonic bath for homogenization. Due to the degradation of grape seed extract at higher temperatures, the ultrasonic bath is operated at certain time intervals to prevent the mixture against a possible damage caused by the vibrational heat. After all steps, the xylitol mixture is combined with the grape seed extract mixture, the final volume of 5 mL is completed with distilled water. The solution placed on a magnetic stirrer until homogenized.

[0055] After the production step, the dental varnishes of the present invention are bottled, and the lids of the bottles are stabilized with paraffin tapes for a safe transportation. After packaging, the varnishes are kept in the refrigerator at +4 °C until their usage.

[0056] Example 3: Determination of Cytotoxicity and Biocompatibility Properties of Dental Varnishes Containing Grape Seed Extract Example 3a: Cytotoxicity Study of Experimental Varnishes Containing Grape Seed Extract (In Vitro Cytotoxicity Test, Direct Contact Analysis)

[0057] In vitro cell viability is evaluated by direct contact test according to ISO 10993-5 standard. The L929 cell line (healthy mice fibroblast cells) is obtained from ATCC (American Type Culture Collection). L929 cells are supplied with 10% (v / v) FBS (Fetal Bovine Serum) and 1% penicillin (10,000 units / mL) and streptomycin (10,000 pg / mL) and cultured in DMEM (Dulbecco's Modified Eagle's Medium / High glucose) in a cell culture incubator at 37 °C and in the presence of 5% CO2. The cells are planted in a medium in 24-well plates with 55,000 cells in each well and left to incubate in a drying-oven at 37 °C for 24 hours and in the presence of 5% CO2for adhesion. At the end of 24 hours of incubation, solutions (20% grape seed extract, 10% xylitol + 10% grape seed extract, 20% xylitol + 20% grape seed extract) are applied to the surface of a filter paper with a pore size of 0.45 pm for a direct contact, and filter papers are placed in the holes on the cell surface. In the experiment, PBS (Gibco, USA) was used as the negative control and 0.4 mg / mL SDS (Merck, Germany) was used as the positive control. Filter papers are prepared to correspond to a 1 :10 surface of the well area and sterilized. After 24 hours, the cell medium and filter papers are removed from the wells. Then, MTT (Sigma-Aldrich, Germany) solution (0.5 mg / mL) is added to the wells and the cells are further incubated at 37 °C for another 2 hours. After 2 hours of incubation, the cell culture medium is removed and 100 pl of isopropanol (Sigma-Aldrich, Germany) is added to the wells to dissolve the formazan. The absorbance is measured with an ELISA microplate reader (BioTek, USA) at a wavelength of 570 nm. The decrease in viability compared to the negative control is calculated using the following equation:

[0058] Cell Viability % = ((OD570 (sample) / OD570 (negative control)) x 100

[0059] Example 3b: HET-CAM Test of Experimental Varnishes Containing Grape Seed Extract

[0060] For the test, fresh (maximum 7 days old) white Leghorn chicken eggs weighing 50-60 grams were used. Before incubation, the eggs were checked with a light and the damaged ones were excluded from the experiment. Eggs suitable for testing were incubated at 38.3 ± 0.2 °C for 9 days in an environment with 58 ± 2 humidity. On day 9, the air gap on the eggs was determined using a light in a dark environment and marked. Then the area of the air gap was cut off. The inner membrane was moistened with 0.9% NaCI with a disposable glass pipette and placed in an incubator for 30 minutes. After the eggs were taken out of the incubator, the 0.9% NaCI solution thereon was cleaned and the inner membrane was removed with a forceps.

[0061] In the HET-CAM test, 0.9% NaCI is used as the negative control and NaOH at 0.1 normality, which is an irritant, is used as the positive control. 3 repetitions were performed for each group. Since the substances to be tested are semi-solid, 0.3 grams were weighed and applied. Reactions occurring after the application were observed for 300 seconds.

[0062] In the evaluation step of the results, scoring was performed for each group according to their hemorrhage, lysis, and coagulation potentials at 0.5. - 2. and 5. minutes and it was decided whether the test substances were irritant or not according to the scoring result. The maximum irritation score (IS) for irritation potential was determined as 21 according to ICCVAM. For a valid test, the IS value must be 0 for 0.9% NaCI solution used as a negative control, and between values of 10 and 19 for the positive control group as NaOH at 0.1 normality.

[0063] Table 1 : HET-CAM Irritation Test Damage Scoring for Groups.

[0064] Effect Score

[0065] 0.5 minutes 2 minutes 5 minutes

[0066] Vascular Lysis 5 3 1

[0067] Hemorrhage 7 5 3

[0068] Coagulation 9 7 5

[0069] Formation

[0070] Example 4: Results

[0071] Example 4a: Cytotoxicity Result of Direct Contact Analysis

[0072] The cell viability rate for the 10% Xylitol + 10% Grape Seed Extract Varnish group was found to be over 70% compared to the negative control group, and it was decided that the formulation created with these percentages is not cytotoxic. On the other hand, 20% Grape Seed Extract Varnish and 20% Xyliol + 20% Grape Seed Extract Varnish were evaluated as cytotoxic regarding to the obtained results, as the cell viability was below 70% compared to the negative control group. After 24 hours of contact, the cell viability in the L929 cell line is as follows: for 20% Grape Seed Extract Varnish, 10% Xylitol + 10% Grape Seed Extract Varnish, and 20% Xylitol + 20% Grape Seed Extract Varnish, respectively: 55.23 ± 2.75%, 91.17 ± 1.5%, and 51 .46 ± 2.06%. The test results are given in Figure 1 .

[0073] Example 4b: HET-CAM Test Result

[0074] Considering the results, the IS value of the negative control group (0.9% NaCI) was scored as 0, where the IS value of the positive control group (NaOH at 0.1 normality) as 19. The score for 10% Xylitol + 10% Grape Seed Extract Varnish, 20% Grape Seed Extract Varnish, and 20% Xylitol + 20% Grape Seed Extract Varnish was calculated as 0. These results are within the acceptable range according to the ICCVAM protocol. None of the analyzed test substances caused lysis, bleeding and clotting, therefore they were concluded as non-irritants according to the HET-CAM scoring.

[0075] Table 2: HET-CAM Irritation Test Damage Scoring for Positive Control (NaOH at 0.1 normality).

[0076] Effect Score=19

[0077] 0.5 minutes 2 minutes 5 minutes

[0078] Vascular Lysis 5

[0079] Hemorrhage 7

[0080] Coagulation 7 Formation

Claims

CLAIMS1. A fluoride-free dental varnish with surface and subsurface remineralization activity containing grape seed extract, providing prevention and treatment of the formation of dental caries in applications in the field of dental technology.

2. A dental varnish according to claim 1 , comprising grape seed extract as a component at a value in the range of 5% to 25% by weight.

3. A dental varnish according to claim 2, comprising grape seed extract as a component at a value in the range of 10% to 20% by weight.

4. A dental varnish according to one of the preceding claims, comprising at least one natural sugar alcohol at a value in the range of 5% to 25% by weight and grape seed extract.

5. A dental varnish according to one of the preceding claims, comprising at least one natural sugar alcohol at a value in the range of 10% to 20% by weight and grape seed extract.

6. A dental varnish according to one of the preceding claims, comprising at least one of the xylitol and / or erythritol compounds as a natural sugar alcohol.

7. A dental varnish according to claim 6, comprising the xylitol compound as a natural sugar alcohol.

8. A fluoride-free dental varnish with surface and subsurface remineralization activity and providing prevention of dental caries formation and treatment of dental caries in applications in the field of dental technology, characterized in that it comprises the following process steps of:Adding grape seed extract into at least one alcohol-based solvent and subjecting it to mixing processes,Forming a solution with xanthan gum with at least one solvent,Performing mixing operations by combining the obtained grape seed extract- at least one alcohol-based solvent and xanthan gum-at least one solvent solutions.

9. A method according to claim 8, characterized in that the solvent is distilled water.

10. A method according to one of claims 8-9, characterized in that the thickening agent or thickener component comprises at least one selected from the group of gellan gum, pectin, resins, xanthan gum.

11. A method according to one of claims 8-10, characterized in that alcohol-based solvent comprises ethanol.

12. A method according to one of claims 8-11 , characterized in that a sugar alcohol is added into the solvent such that it is at a value in the range of 5% to 25% by weight in the final product dental varnish.

13. A method according to one of claims 8-12, characterized in that the grape seed extract is added into the alcohol-based solvent such that it is at a value in the range of 5% to 25% by weight in the final product dental varnish.

14. Method according to one of the preceding claims, characterized in that it comprises the following process steps of:- Mixing and homogenizing at least one sugar alcohol in at least one solvent by means of a stirrer,- Adding a thickening agent or thickener component to the homogeneous mixture obtained and repeating the stirring process,- Adding grape seed extract into at least one alcohol-based solvent and performing the stirring processes,Forming a new solution by combining obtained at least one grape seed extract solution with alcohol-based solvent and sugar alcohol and mixing this solution with a stirrer,- Adding distilled water to the obtained solution.

15. A method according to claim 14, characterized in that it comprises at least one of the xylitol and / or erythritol compounds as said sugar alcohol.

16. A method according to claim 15, characterized in that the sugar alcohol is xylitol.

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