Non-contact biological particle treatment device and biological particle treatment apparatus

Abstract

A non-contact biological particle treatment device and a biological particle treatment apparatus. The biological particle treatment apparatus comprises a droplet generation chamber, a working chamber in communication with the droplet generation chamber, and a sorting chamber in communication with the working chamber. The droplet generation chamber is configured for receiving a first liquid, a biological particle located within the first liquid, and a second liquid immiscible in the first liquid. The droplet generation chamber is configured for staggering the flow of the second liquid with the first liquid, so that the biological particle and the first liquid portion around the biological particle jointly generate, after passing through the second liquid, a biological particle droplet flowing to the working chamber, and the biological particle is cultured or tested by means of the first liquid. Thus, the biological particle droplet can protect at least one of the biological particles located therein, and complete a culture operation or detection while moving.

Description

Non-contact biological particulate treatment equipment and biological particulate treatment devices

[0001] This application claims priority to U.S. Provisional Patent Application No. 63 / 665259, filed June 28, 2024, entitled “Non-contact bioparticle treatment device and bioparticle treatment apparatus,” the entire contents of which are incorporated herein by reference. Technical Field

[0002] This invention relates to a biological particle treatment system, and more particularly to a non-contact biological particle treatment device and biological particle treatment apparatus. Background Technology

[0003] Existing microparticle processing systems can process microparticles within a liquid. However, because the microparticles move with the liquid, these systems typically employ a fixed-point method to immobilize them, which is detrimental to microparticle cultivation. Furthermore, whether the microparticles are moving with the liquid or immobilized, they are susceptible to pressure fluctuations and damage from the liquid.

[0004] Therefore, the inventor believed that the above-mentioned defects could be improved, and thus devoted himself to research and applied scientific principles, and finally proposed an invention that is reasonably designed and effectively improves the above-mentioned defects. Summary of the Invention

[0005] The present invention provides a non-contact biological particle treatment device and a biological particle treatment apparatus, which can effectively improve the defects that may be generated by existing biological particle treatment systems.

[0006] This invention discloses a non-contact bioparticle processing device, comprising: a bioparticle processing apparatus for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the bioparticle processing apparatus includes: a droplet generation chamber for containing the first liquid and at least one bioparticle located within the first liquid; wherein the droplet generation chamber is used to generate a bioparticle droplet by the at least one bioparticle and a portion of the surrounding first liquid; and a working chamber connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid and the bioparticle droplet, so that the bioparticle droplet can flow within the second liquid in the working chamber, and the bioparticle droplet... A first liquid is used to perform a culture or detection operation on at least one biological microparticle; a sorting chamber is connected to the working chamber; wherein the sorting chamber is used to contain the first liquid to create an immiscible interface between the working chamber and the sorting chamber; a photo-driven device is facing the biological microparticle processing device; wherein the photo-driven device is used to drive the biological microparticle processing device to generate a dielectrophoretic pattern to move the biological microparticle droplets; wherein the photo-driven device can use the dielectrophoretic pattern to move the biological microparticle droplets from the working chamber to the sorting chamber, such that the first liquid of the biological microparticle droplets dissolves into the first liquid of the sorting chamber, thereby releasing at least one biological microparticle into the first liquid of the sorting chamber.

[0007] Optionally, the droplet generation chamber is used to contain the second liquid and to allow the flow of the second liquid to interweave with the first liquid, so that at least one biological microparticle and a portion of the first liquid around it, after passing through the second liquid, jointly generate biological microparticle droplets.

[0008] Optionally, the droplet generation chamber includes: a first flow channel for introducing a first liquid and at least one biological microparticle; and a second flow channel intersecting the first flow channel to generate a confluence region communicating with the working chamber; wherein the second flow channel is used to introduce a second liquid, and at least one biological microparticle and a portion of the first liquid around it jointly generate biological microparticle droplets after passing through the confluence region.

[0009] Optionally, the working chamber is provided with a waste outlet, and the photo-driven device can selectively move the biological microparticle droplets from the working chamber to the sorting chamber or the waste outlet by using a dielectrophoretic pattern.

[0010] Optionally, the first liquid of the bioparticle droplet contains at least one of a culture medium, a peptide, and a recombinant protein for culturing at least one bioparticle; or, the first liquid of the bioparticle droplet contains at least one of a detection reagent and a chemical reagent for detecting at least one bioparticle.

[0011] Optionally, the bioparticle processing device includes: a photosensitive module comprising a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; a mating module spaced apart from the photosensitive module, wherein at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the photosensitive module; wherein the light driving device can be used to emit light to irradiate the photosensitive module, so that the photosensitive module generates a dielectric electrophoretic pattern.

[0012] This invention also discloses a non-contact bioparticle processing device, comprising: a bioparticle processing apparatus for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the bioparticle processing apparatus includes: a droplet generation chamber for containing the first liquid and at least one bioparticle located within the first liquid; wherein the droplet generation chamber is used to generate a bioparticle droplet by the at least one bioparticle and a portion of the surrounding first liquid; and a working chamber connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid and the bioparticle droplet, so that the bioparticle droplet can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplet is used to treat... At least one bioparticle undergoes a culture or detection process; a sorting chamber is connected to a processing chamber and has a release structure formed at the edge adjacent to the processing chamber; wherein the sorting chamber is used to contain a second liquid; a photo-driven device is directed toward the bioparticle processing device; wherein the photo-driven device is used to drive the bioparticle processing device to generate a dielectrophoretic pattern to move bioparticle droplets; wherein the photo-driven device is used to move bioparticle droplets from the processing chamber along the release structure to the sorting chamber via the dielectrophoretic pattern, such that the bioparticle droplets are disrupted by the release structure, thereby dispersing the first liquid of the bioparticle droplets and releasing at least one bioparticle into the second liquid in the sorting chamber.

[0013] Optionally, the droplet generation chamber is used to contain the second liquid and to allow the flow of the second liquid to interweave with the first liquid, so that at least one biological microparticle and a portion of the first liquid around it, after passing through the second liquid, jointly generate biological microparticle droplets.

[0014] Optionally, the droplet generation chamber includes: a first flow channel for introducing a first liquid and at least one biological microparticle; and a second flow channel intersecting the first flow channel to generate a confluence region communicating with the working chamber; wherein the second flow channel is used to introduce a second liquid, and at least one biological microparticle and a portion of the first liquid around it jointly generate biological microparticle droplets after passing through the confluence region.

[0015] Optionally, the working chamber is provided with a waste outlet, and the photo-driven device can selectively move the biological microparticle droplets from the working chamber to the sorting chamber or the waste outlet by using a dielectrophoretic pattern.

[0016] Optionally, the first liquid of the bioparticle droplet contains at least one of a culture medium, a peptide, and a recombinant protein for culturing at least one bioparticle; or, the first liquid of the bioparticle droplet contains at least one of a detection reagent and a chemical reagent for detecting at least one bioparticle.

[0017] Optionally, the bioparticle processing device includes: a photosensitive module comprising a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; a mating module spaced apart from the photosensitive module, wherein at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; wherein the light driving device can be used to emit light to irradiate the photosensitive module, so that the photosensitive module generates a dielectric electrophoretic pattern.

[0018] Optionally, the density of the first liquid is greater than the density of the second liquid, and the release structure is formed in the insulating layer.

[0019] Optionally, the density of the first liquid is less than the density of the second liquid, and the release structure is generated in the mating module.

[0020] This invention also discloses a bioparticle processing device for receiving a first liquid and a second liquid immiscible with the first liquid. The bioparticle processing device includes: a droplet generation chamber for containing the first liquid, at least one bioparticle located within the first liquid, and the second liquid; wherein the droplet generation chamber is used to allow the flow of the second liquid to interweave with the first liquid, so that at least one bioparticle and the portion of the first liquid surrounding it, after passing through the second liquid, jointly generate a bioparticle droplet; a working chamber connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid, so that the bioparticle droplet can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplet is used to perform a culture operation or a detection operation on at least one bioparticle; and a sorting chamber connected to the working chamber.

[0021] Optionally, the sorting chamber is used to contain a first liquid to create an immiscible interface between the working chamber and the sorting chamber; wherein, when the bioparticle droplets move from the working chamber to the sorting chamber, the first liquid of the bioparticle droplets dissolves into the first liquid of the sorting chamber to release at least one bioparticle into the first liquid of the sorting chamber.

[0022] Optionally, the sorting chamber is used to contain a second liquid, and a release structure is formed at the edge of the sorting chamber adjacent to the working chamber; wherein, when the bioparticle droplets move from the working chamber to the sorting chamber via the release structure, the bioparticle droplets are disrupted by the release structure, so that the first liquid of the bioparticle droplets is dispersed, thereby releasing at least one bioparticle into the second liquid of the sorting chamber.

[0023] Optionally, the bioparticle processing device includes: a photosensitive module comprising a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; a mating module spaced apart from the photosensitive module, wherein at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; wherein the density of the first liquid is greater than the density of the second liquid, and the release structure is formed on the insulating layer.

[0024] Optionally, the bioparticle processing device includes: a photosensitive module comprising a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; a mating module spaced apart from the photosensitive module, wherein at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; wherein the density of the first liquid is less than the density of the second liquid, and a release structure is formed in the mating module.

[0025] Optionally, the droplet generation chamber includes: a first flow channel for introducing a first liquid and at least one biological microparticle; and a second flow channel intersecting the first flow channel to generate a confluence region communicating with the working chamber; wherein the second flow channel is used to introduce a second liquid, and at least one biological microparticle and a portion of the first liquid around it jointly generate biological microparticle droplets after passing through the confluence region.

[0026] In summary, the non-contact biological particle processing device and biological particle processing apparatus disclosed in the embodiments of the present invention can achieve a protective effect by generating biological particle droplets suspended in the second liquid, so that at least one biological particle is covered in the first liquid. This allows the biological particle droplets to move rapidly within the second liquid without harming at least one biological particle located therein. Furthermore, the biological particle droplets can complete the cultivation or detection of at least one biological particle located therein while moving.

[0027] To further understand the features and technical content of this invention, please refer to the following detailed description and accompanying drawings. However, these descriptions and drawings are only for illustrating the invention and are not intended to limit the scope of protection of the invention in any way. Attached Figure Description

[0028] Figure 1 is a three-dimensional schematic diagram of the non-contact biological microparticle treatment device according to Embodiment 1 of the present invention.

[0029] Figure 2 is a longitudinal cross-sectional view of the non-contact biological microparticle treatment device shown in Figure 1.

[0030] Figure 3 is a cross-sectional schematic diagram of the non-contact biological microparticle treatment device according to Embodiment 1 of the present invention.

[0031] Figure 4 is a cross-sectional schematic diagram of the non-contact bioparticle processing device in Figure 3 generating multiple bioparticle droplets.

[0032] Figure 5 is a longitudinal cross-sectional schematic diagram of the non-contact biological microparticle treatment device according to Embodiment 1 of the present invention.

[0033] Figure 6 is a schematic diagram of the subsequent operation of Figure 3.

[0034] Figure 7 is a schematic diagram of the subsequent operation of Figure 6.

[0035] Figure 8 is a cross-sectional schematic diagram of another aspect of the non-contact biological particle treatment device according to Embodiment 1 of the present invention.

[0036] Figure 9 is a cross-sectional schematic diagram of the non-contact biological microparticle treatment device according to Embodiment 2 of the present invention.

[0037] Figure 10 is a longitudinal cross-sectional schematic diagram of the non-contact biological microparticle treatment device shown in Figure 9.

[0038] Figure 11 is a schematic diagram of the subsequent operation of Figure 9.

[0039] Figure 12 is a longitudinal cross-sectional view of the non-contact biological microparticle treatment device in Figure 11.

[0040] Figure 13 is a schematic diagram of the subsequent operation of Figure 11.

[0041] Figure 14 is a longitudinal cross-sectional view of the non-contact biological microparticle treatment device shown in Figure 13.

[0042] Figure 15 is a cross-sectional schematic diagram of another aspect of the non-contact biological particle treatment device according to Embodiment 2 of the present invention.

[0043] Figure 16 is a longitudinal cross-sectional view of the non-contact biological microparticle treatment device in Figure 15.

[0044] Figure 17 is a schematic diagram of the subsequent operation of Figure 15.

[0045] Figure 18 is a longitudinal cross-sectional view of the non-contact biological microparticle treatment device shown in Figure 17. Detailed Implementation

[0046] The following specific embodiments illustrate the implementation of the "non-contact biological particle treatment device and biological particle treatment apparatus" disclosed in this invention. Those skilled in the art can understand the advantages and effects of this invention from the content disclosed in this specification. This invention can be implemented or applied through other different specific embodiments, and various details in this specification can also be modified and changed based on different viewpoints and applications without departing from the concept of this invention. Furthermore, the accompanying drawings of this invention are for simple illustrative purposes only and are not depictions of actual dimensions; this is stated in advance. The following embodiments will further describe the relevant technical content of this invention in detail, but the disclosed content is not intended to limit the scope of protection of this invention.

[0047] It should be understood that while terms such as "first," "second," and "third" may be used in this document to describe various elements or features, these elements or features should not be limited by these terms. These terms are primarily used to distinguish one element from another, or one feature from another. Furthermore, the term "or" as used herein may, as appropriate, include any combination of one or more of the related listed items.

[0048] [Example 1]

[0049] Please refer to Figures 1 to 8, which illustrate Embodiment 1 of the present invention. As shown in Figures 1 to 3, this embodiment discloses a non-contact biological particle processing device 100, used to perform a culture or detection operation on at least one biological particle B. The biological particle B can be a specific type of cell or cell cluster, such as circulating tumor cells (CTCs), fetal nucleated red blood cells (FNRBCs), viruses, microorganisms, or bacteria, but the present invention is not limited to the above.

[0050] The non-contact bioparticle processing device 100 includes a bioparticle processing unit 1, an AC power device 2 electrically coupled to the bioparticle processing unit 1, and a light-driven device 3 facing the bioparticle processing unit 1, but the present invention is not limited thereto. For example, in other embodiments of the present invention not shown, the bioparticle processing unit 1 can be used independently (e.g., for sale) or in conjunction with other devices as needed.

[0051] In this embodiment, the bioparticle processing device 1 is a rectangular structure with a chip-scale design. The bioparticle processing device 1 is used to receive (or contain) a first liquid L1, at least one bioparticle B located within the first liquid L1, and a second liquid L2 immiscible with the first liquid L1. For example, the first liquid L1 may contain oil and a surfactant, while the second liquid L2 is water; or, the first liquid L1 may contain water and a surfactant, while the second liquid L2 is oil, but the invention is not limited thereto.

[0052] Furthermore, the light-driven device 3 can be used to drive the bioparticle processing device 1 to generate a dielectrophoresis (DEP) pattern F (as shown in Figure 6). The structure of the bioparticle processing device 1 that can be used to realize the dielectrophoresis pattern F is generally described below, but the present invention is not limited thereto.

[0053] In this embodiment, the bioparticle processing device 1 includes a photosensitive module 11, a mating module 12 spaced apart from the photosensitive module 11, and an adhesive layer 13 bonding the periphery of the photosensitive module 11 and the periphery of the mating module 12. At least one of the photosensitive module 11 and the mating module 12 is transparent, and in this embodiment, the photosensitive module 11 and the mating module 12 are two plate-like structures arranged parallel to each other, with a distance between them greater than the size of any one of the bioparticles B. However, the present invention is not limited to the above description.

[0054] More specifically, the photosensitive module 11 includes a first substrate 111, a first electrode layer 112 formed on the first substrate 111, and a photoelectric layer 113 formed on the first substrate 111. In this embodiment, the first electrode layer 112 is formed on the bottom side of the first substrate 111, and the photoelectric layer 113 is formed on the top side of the first substrate 111. The photoelectric layer 113 may have a plurality of transistors arranged in a matrix, and the photoelectric layer 113 may adopt an NPN transistor architecture, a PNP transistor architecture, an NP diode architecture, or a PN diode architecture according to actual needs, but the present invention is not limited thereto.

[0055] The mating module 12 includes a second substrate 121 and a second electrode layer 122 formed on the second substrate 121, and the second electrode layer 122 faces the photosensing module 11 (e.g., the photoelectric layer 113). The AC device 2 is electrically coupled to the first electrode layer 112 of the photosensing module 11 and the second electrode layer 122 of the mating module 12.

[0056] Therefore, as shown in Figures 2 and 5 to 7, the light-driving device 3 can emit light to illuminate the photosensitive module 11, so that the photosensitive module 11 generates the dielectric electrophoretic pattern F. In this embodiment, the light-driving device 3 may include a camera 31 and a light source 32 associated with the camera 31. The light-driving device 3 can emit light through the light source 32 to illuminate the photosensitive module 11, so that the photosensitive module 11 (or the photoelectric layer 113) generates the dielectric electrophoretic pattern F.

[0057] From another perspective, the internal structure of the bioparticle processing device 1 includes a droplet generation chamber 14, a working chamber 15 connected to the droplet generation chamber 14, and a sorting chamber 16 connected to the working chamber 15. In this embodiment, the droplet generation chamber 14, the working chamber 15, and the sorting chamber 16 are disposed between the photosensitive module 11 and the cooperation module 12, and the droplet generation chamber 14 and the sorting chamber 16 are respectively connected to opposite sides of the working chamber 15, but the invention is not limited thereto.

[0058] The droplet generation chamber 14 is used to contain the first liquid L1 and at least one of the biological particles B located within the first liquid L1. The droplet generation chamber 14 is used to generate a biological particle droplet P by at least one of the biological particles B and a portion of the surrounding first liquid L1.

[0059] It should be noted that, for ease of understanding of this embodiment, the following description will assume that only one biological microparticle droplet P is generated within the biological microparticle processing device 1, but the present invention is not limited thereto. For example, as shown in FIG4, multiple biological microparticle droplets P may exist simultaneously within the biological microparticle processing device 1, and the number of biological microparticles B within any biological microparticle droplet P may be greater than one depending on actual needs.

[0060] Furthermore, provided that the biological microparticle droplets P can be generated, the droplet generation chamber 14 can be designed according to actual needs. For example, in other embodiments not shown in this invention, the droplet generation chamber 14 can disperse the first liquid L1 into multiple droplets by physical means (e.g., stirring or shaking), and the droplet coated with at least one of the biological microparticles B is defined as the biological microparticle droplet P.

[0061] Furthermore, in this embodiment, the droplet generation chamber 14 generates the bioparticle droplet P via a fluid method to reduce potential damage to the bioparticle B. Specifically, the droplet generation chamber 14 further accommodates the second liquid L2, and the flow of the second liquid L2 interweaves with the first liquid L1, so that at least one bioparticle B and a portion of the surrounding first liquid L1, after passing through the second liquid L2, jointly generate the bioparticle droplet P.

[0062] More specifically, the droplet generation chamber 14 includes a first flow channel 141 and a second flow channel 142 (vertically) intersecting the first flow channel 141, and the first flow channel 141 and the second flow channel 142 intersect each other to generate a confluence region 143 communicating with the working chamber 15. The first flow channel 141 is used to input the first liquid L1 and at least one of the biological particles B, and the second flow channel 142 is used to input the second liquid L2, so that at least one of the biological particles B and the surrounding portion of the first liquid L1, after passing through the confluence region 143, jointly generate the biological particle droplet P.

[0063] The working chamber 15 is used to contain the second liquid L2 and the bioparticle droplet P, so that the bioparticle droplet P can flow within the second liquid L2 in the working chamber 15, and can also be moved (e.g., pushed) within the working chamber 15 by the dielectrophoretic pattern F. Furthermore, the bioparticle droplet P located in the working chamber 15 can be used to perform a culture or detection operation on at least one of the bioparticles B within the first liquid L1.

[0064] In this embodiment, the first liquid L1 of the biological microparticle droplet P contains at least one of a culture medium, a peptide, and a recombinant protein for culturing at least one of the biological microparticles B; or, the first liquid L1 of the biological microparticle droplet P contains at least one of a detection reagent and chemicals for detecting at least one of the biological microparticles B.

[0065] As described above, in this embodiment, the biological microparticle processing device 1 can achieve a protective effect by generating biological microparticle droplets P suspended in the second liquid L2, so that at least one biological microparticle B is covered in the first liquid L1. This allows the biological microparticle droplets P to move rapidly within the second liquid L2 without harming at least one biological microparticle B. Furthermore, the biological microparticle droplets P can complete the cultivation or detection of at least one biological microparticle B while moving.

[0066] It should be noted that the working chamber 15 has a waste outlet 151. The light-driven device 3 can selectively move the biological microparticle droplet P from the working chamber 15 to the sorting chamber 16 or the waste outlet 151 through the dielectrophoresis pattern F. That is, after the biological microparticle droplet P has undergone the cultivation or detection process, if the result is unsuccessful, the biological microparticle droplet P will be moved to the waste outlet 151 through the dielectrophoresis pattern F and then removed from the biological microparticle processing device 1; if the result is successful, the biological microparticle droplet P will be moved into the sorting chamber 16 through the dielectrophoresis pattern F.

[0067] Furthermore, the sorting chamber 16 is used to contain the first liquid L1, so that an immiscible interface L3 is formed between the working chamber 15 and the sorting chamber 16. Thus, the photo-driving device 3 can use the dielectrophoretic pattern F to move the biological microparticle droplet P from the working chamber 15 to the sorting chamber 16, causing the first liquid L1 of the biological microparticle droplet P to dissolve into the first liquid L1 of the sorting chamber 16, thereby releasing at least one biological microparticle B into the first liquid L1 of the sorting chamber 16.

[0068] Furthermore, the sorting chamber 16 may also have a collection port 161, through which at least one of the biological particles B located within the sorting chamber 16 can be removed from the biological particle processing device 1. The movement of at least one of the biological particles B within the sorting chamber 16 can be achieved by the dielectrophoretic pattern F (e.g., Figures 6 and 7) or by hydraulic control (e.g., Figure 8 further provides a hydraulic control port 163).

[0069] [Example 2]

[0070] Please refer to Figures 9 to 18, which illustrate Embodiment 2 of the present invention. Since this embodiment is similar to Embodiment 1 described above, the similarities between the two embodiments will not be repeated. The main difference between this embodiment and Embodiment 1 lies in the sorting chamber 16.

[0071] In this embodiment, the sorting chamber 16 is used to contain the second liquid L2, the photosensitive module 11 includes an insulating layer 114 formed on the photoelectric layer 113, and the sorting chamber 16 has a release structure 162 formed at the edge adjacent to the working chamber 15 for disrupting the surface tension of the bioparticle droplet P. In this embodiment, the release structure 162 includes a plurality of protrusions 1621 arranged along the edge of the working chamber 15, and the distance between any two adjacent protrusions 1621 may be smaller than the outer diameter of the bioparticle droplet P, but the invention is not limited thereto.

[0072] It should be noted that, as shown in Figures 9 to 14, when the density of the first liquid L1 used in the bioparticle processing device 1 is greater than the density of the second liquid L2, the bioparticle droplets P tend to sink within the second liquid L2, and therefore the release structure 162 is formed on the insulating layer 114. Furthermore, as shown in Figures 15 to 18, when the density of the first liquid L1 used in the bioparticle processing device 1 is less than the density of the second liquid L2, the bioparticle droplets P tend to float within the second liquid L2, and therefore the release structure 162 is formed on the mating module 12.

[0073] As described above, the light-driven device 3 can use the dielectric electrophoresis pattern F to move the biological microparticle droplet P from the working chamber 15 along the release structure 162 to the sorting chamber 16, so that the biological microparticle droplet P is destroyed by the release structure 162, thereby dispersing the first liquid L1 of the biological microparticle droplet P and releasing at least one biological microparticle B into the second liquid L2 of the sorting chamber 16.

[0074] [Technical Effects of the Embodiments of the Invention]

[0075] In summary, the non-contact biological particle processing device and biological particle processing apparatus disclosed in the embodiments of the present invention can achieve a protective effect by generating biological particle droplets suspended in the second liquid, so that at least one biological particle is covered in the first liquid. This allows the biological particle droplets to move rapidly within the second liquid without harming at least one biological particle located therein. Furthermore, the biological particle droplets can complete the cultivation or detection of at least one biological particle located therein while moving.

[0076] The above-disclosed content is only an optional and feasible embodiment of the present invention, and is not intended to limit the patent scope of the present invention. Therefore, all equivalent technical changes made based on the content of the present invention specification and drawings are included within the patent scope of the present invention.

Claims

1. A non-contact biological particle treatment device, characterized in that, The non-contact biological particle treatment device includes: A bioparticle processing device for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the bioparticle processing device comprises: A droplet generation chamber for containing the first liquid and at least one biological microparticle located within the first liquid; wherein the droplet generation chamber is used for at least one of the biological microparticles to generate biological microparticle droplets together with the surrounding portion of the first liquid; A working chamber, connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid and the bioparticle droplets, so that the bioparticle droplets can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplets is used to perform culturing or detection operations on at least one of the bioparticles; and A sorting chamber is connected to the working chamber; wherein the sorting chamber is used to contain the first liquid, so that an immiscible interface is formed between the working chamber and the sorting chamber; and A light-driven device facing the bioparticle processing device; wherein the light-driven device can be used to drive the bioparticle processing device to generate a dielectrophoretic pattern to move the bioparticle droplets. The light-driven device can move the biological microparticle droplets from the working chamber to the sorting chamber through the dielectric electrophoresis pattern, so that the first liquid of the biological microparticle droplets dissolves into the first liquid of the sorting chamber, thereby releasing at least one biological microparticle into the first liquid of the sorting chamber.

2. The non-contact biological particle treatment device according to claim 1, characterized in that, The droplet generation chamber is used to contain the second liquid and to allow the flow of the second liquid to interweave with the first liquid, so that at least one of the biological microparticles and the portion of the first liquid surrounding them, after passing through the second liquid, jointly generate the biological microparticle droplets.

3. The non-contact biological particle treatment device according to claim 2, characterized in that, The droplet generation chamber includes: A first flow channel is used for introducing the first liquid and at least one of the said biological particles; and A second flow channel, intersecting the first flow channel, forms a confluence region communicating with the working chamber; wherein the second flow channel is used to introduce the second liquid and cause at least one of the bioparticles and the portion of the first liquid surrounding them to co-generate the bioparticle droplets after passing through the confluence region.

4. The non-contact biological particle treatment device according to claim 1, characterized in that, The working chamber has a waste outlet, and the photo-driven device can selectively move the biological microparticle droplets from the working chamber to the sorting chamber or the waste outlet using the dielectric electrophoresis pattern.

5. The non-contact biological particle treatment device according to claim 1, characterized in that, The first liquid of the bioparticle droplet contains at least one of a culture medium, a peptide, and a recombinant protein for culturing at least one of the bioparticles; or, the first liquid of the bioparticle droplet contains at least one of a detection reagent and a chemical reagent for detecting at least one of the bioparticles.

6. The non-contact biological particle treatment device according to claim 1, characterized in that, The bioparticle processing device comprises: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, and a photoelectric layer formed on the first substrate; and A mating module is spaced apart from the photosensing module, and at least one of the photosensing module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate, and the second electrode layer faces the photosensing module; The light-driving device can be used to emit light to illuminate the light-sensing module, so that the light-sensing module generates the dielectric electrophoretic pattern.

7. A non-contact biological particle treatment device, characterized in that, The non-contact biological particle treatment device includes: A bioparticle processing device for receiving a first liquid and a second liquid immiscible with the first liquid; wherein the bioparticle processing device comprises: A droplet generation chamber for containing the first liquid and at least one biological microparticle located within the first liquid; wherein the droplet generation chamber is used for at least one of the biological microparticles to generate biological microparticle droplets together with the surrounding portion of the first liquid; A working chamber, connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid and the bioparticle droplets, so that the bioparticle droplets can flow within the second liquid in the working chamber, and the first liquid of the bioparticle droplets is used to perform culturing or detection operations on at least one of the bioparticles; and A sorting chamber, connected to the working chamber and having a release structure formed at its edge adjacent to the working chamber; wherein the sorting chamber is used to contain the second liquid; and A light-driven device facing the bioparticle processing device; wherein the light-driven device can be used to drive the bioparticle processing device to generate a dielectrophoretic pattern to move the bioparticle droplets. The light-driven device can use the dielectric electrophoresis pattern to move the biological microparticle droplets from the working chamber to the sorting chamber along the release structure, so that the biological microparticle droplets are destroyed by the release structure, thereby dispersing the first liquid of the biological microparticle droplets and releasing at least one biological microparticle into the second liquid of the sorting chamber.

8. The non-contact biological particle treatment device according to claim 7, characterized in that, The droplet generation chamber is used to contain the second liquid and to allow the flow of the second liquid to interweave with the first liquid, so that at least one of the biological microparticles and the portion of the first liquid surrounding them, after passing through the second liquid, jointly generate the biological microparticle droplets.

9. The non-contact biological particle treatment device according to claim 8, characterized in that, The droplet generation chamber includes: A first flow channel is used for introducing the first liquid and at least one of the said biological particles; and A second flow channel, intersecting the first flow channel, forms a confluence region communicating with the working chamber; wherein the second flow channel is used to introduce the second liquid and cause at least one of the bioparticles and the portion of the first liquid surrounding them to co-generate the bioparticle droplets after passing through the confluence region.

10. The non-contact biological particle treatment device according to claim 7, characterized in that, The working chamber has a waste outlet, and the photo-driven device can selectively move the biological microparticle droplets from the working chamber to the sorting chamber or the waste outlet using the dielectric electrophoresis pattern.

11. The non-contact biological particle treatment device according to claim 7, characterized in that, The first liquid of the bioparticle droplet contains at least one of a culture medium, a peptide, and a recombinant protein for culturing at least one of the bioparticles; or, the first liquid of the bioparticle droplet contains at least one of a detection reagent and a chemical reagent for detecting at least one of the bioparticles.

12. The non-contact biological particle treatment device according to claim 7, characterized in that, The bioparticle processing device comprises: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; and A mating module is spaced apart from the photosensitive module, and at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; The light-driving device can be used to emit light to illuminate the light-sensing module, so that the light-sensing module generates the dielectric electrophoretic pattern.

13. The non-contact biological particle treatment device according to claim 12, characterized in that, The density of the first liquid is greater than the density of the second liquid, and the release structure is formed in the insulating layer.

14. The non-contact biological particle treatment device according to claim 12, characterized in that, The density of the first liquid is less than the density of the second liquid, and the release structure is generated in the cooperating module.

15. A biological microparticle treatment device, characterized in that, The bioparticle processing device is used to receive a first liquid and a second liquid immiscible with the first liquid, and the bioparticle processing device includes: A droplet generation chamber for containing a first liquid, at least one biological microparticle located within the first liquid, and a second liquid; wherein the droplet generation chamber is configured to allow the flow of the second liquid to interweave with the first liquid, so that at least one of the biological microparticles and the portion of the first liquid surrounding them, after passing through the second liquid, jointly generate biological microparticle droplets; A working chamber, connected to the droplet generation chamber; wherein the working chamber is used to contain the second liquid, allowing the bioparticle droplets to flow within the second liquid in the working chamber, and to perform culturing or detection operations on at least one of the bioparticles using the first liquid of the bioparticle droplets; and The sorting chamber is connected to the working chamber.

16. The biological microparticle treatment device according to claim 15, characterized in that, The sorting chamber is used to contain the first liquid, so that an immiscible interface is formed between the working chamber and the sorting chamber; wherein, when the bioparticle droplet moves from the working chamber to the sorting chamber, the first liquid of the bioparticle droplet dissolves into the first liquid of the sorting chamber, so as to release at least one bioparticle into the first liquid of the sorting chamber.

17. The bioparticle treatment device according to claim 15, characterized in that, The sorting chamber is used to contain the second liquid, and the sorting chamber has a release structure at its edge adjacent to the working chamber; wherein, when the bioparticle droplet moves from the working chamber to the sorting chamber via the release structure, the bioparticle droplet is disrupted by the release structure, thereby dispersing the first liquid of the bioparticle droplet and releasing at least one bioparticle into the second liquid in the sorting chamber.

18. The bioparticle treatment device according to claim 17, characterized in that, The bioparticle processing device comprises: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; and A mating module is spaced apart from the photosensitive module, and at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; The density of the first liquid is greater than that of the second liquid, and the release structure is formed in the insulating layer.

19. The bioparticle treatment device according to claim 17, characterized in that, The bioparticle processing device comprises: A photosensitive module includes a first substrate, a first electrode layer formed on the first substrate, a photoelectric layer formed on the first substrate, and an insulating layer formed on the photoelectric layer; and A mating module is spaced apart from the photosensitive module, and at least one of the photosensitive module and the mating module is transparent; wherein the mating module includes a second substrate and a second electrode layer formed on the second substrate; The density of the first liquid is less than that of the second liquid, and the release structure is generated in the cooperating module.

20. The bioparticle treatment device according to claim 15, characterized in that, The droplet generation chamber includes: A first flow channel is used for introducing the first liquid and at least one of the said biological particles; and A second flow channel, intersecting the first flow channel, forms a confluence region communicating with the working chamber; wherein the second flow channel is used to introduce the second liquid and cause at least one of the bioparticles and the portion of the first liquid surrounding them to co-generate the bioparticle droplets after passing through the confluence region.

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