The invention discloses a fence device for an animal floor rearing experiment. The fence device comprises frame assemblies, sealing board assemblies and U-shaped connecting parts; each frame assembly comprises a first upper cross rod, a first lower cross rod and two first vertical rods which are arranged in parallel, each first vertical rod is provided with a first through hole along the central axis, and the first vertical rods are provided with corresponding first clamping grooves; each sealing board assembly comprises a first sealing board, a second sealing board, a third sealing board and a fourth sealing board, when a fence is assembled, every two adjacent frame assemblies are connected by inserting one U-shaped connecting part into the first through holes of the corresponding first vertical rods, the two side edges of each sealing board assembly are installed in the first clamping grooves of the corresponding first vertical rods in a clamped mode, and a closed space is formed. The fence device for the animal floor rearing experiment has the advantages that the structure is simple, use is convenient, the cost is low, and the sealing property is good; fence spaces with different sizes can be flexibly assembled according to the bred animal species and the growth cycle of animals, and applicability and practicability are improved.