Anti-Mullerian hormone quantitatively detecting method

A quantitative detection method, Mullerian hormone technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of inaccurate detection results, high instrument cost, cumbersome steps, etc., and achieve convenient use and simple operation , the effect of simple steps

Inactive Publication Date: 2019-03-19
CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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  • Abstract
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  • Claims
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Problems solved by technology

[0006] The invention detects the content of AMH by the three-step double-antibody sandwich enzyme-linked immunosorbent method, which solves the technical problems in the prior art such as cumbersome steps, troublesome operation, high instrument cost, and inaccurate detection results.

Method used

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specific Embodiment

[0025] 1. Reagent preparation

[0026]

[0027]

[0028] 2. Reagent preparation (reagent preparation area)

[0029] 1. AMH Calibrators B-F and AMH Quality Controls Ⅰ & Ⅱ: Dissolve AMH Calibrators B-F and AMH Quality Controls Ⅰ & Ⅱ in 1 mL of purified water, shake gently to fully dissolve and mix well before use.

[0030] 2. Washing solution: Dilute the concentrated lotion (25X) with purified water 25 times. If you measure 20mL of concentrated lotion, you need to add 480mL of purified water. The diluted lotion can be stored at room temperature (22-28°C) for 1 month in a sealed container, but in general, it is ready to use first.

[0031] 3. Microwells: Select the number of microwells required for detection. The remaining microwells should be placed in a sealed bag with desiccant, and the sealed bag must be sealed tightly to prevent moisture.

[0032] 3. Sample processing

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Abstract

The invention discloses an anti-Mullerian hormone quantitatively detecting method and relates to the technical field of biological detection. The anti-Mullerian hormone quantitatively detecting methodachieves quantitative detection through three-step double-antibody sandwich enzyme immunoassay, and combining biotin-antibodies with solid matrix antibody-antigen compounds and then with streptavidin-horseradish peroxidase conjugates to accordingly combine antibody-antigen-antibody biotin compound-SHRP (streptavidin-horseradish peroxidase) onto micropores for enzyme-substrate chromogenic reaction; measuring the degree of reaction between enzymes and substrate through dual-wavelength chromatography with a main wavelength of 450 nm and a reference wavelength of 620/630 nm to obtain absorbance value, and then calculating the concentration of AMH (anti-Mullerian hormone) of a sample to be detected according to a standard curve equation. The anti-Mullerian hormone quantitatively detecting method is concise in process, simple in operation, convenient to use and capable of accurately detecting the AMH concentration and achieving quantitative analysis on detecting results.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a quantitative detection method of anti-Müllerian hormone. Background technique [0002] Anti-Mullerian hormone (AMH) is a member of the transforming growth factor β superfamily, which was first discovered by Professor Alfred Jost in 1974. AMH is a dimeric glycoprotein composed of two identical 70KD subunits connected by disulfide bonds, with a relative molecular mass of 140X103Da. The human AMH encoding gene is located on the short arm of chromosome 19, with a size of 2.4-2.8kb and contains 5 exosomes. Exon. [0003] With the change of modern life style, the phenomenon of late marriage and late childbearing is common, unhealthy modern life style (such as excessive dieting, smoking, drinking, staying up late), and huge work pressure have caused the rate of infertility to rise rapidly. Therefore, early assessment of male and female fertility is necessary. Anti-Müll...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/535G01N33/74
CPCG01N33/535G01N33/74
Inventor 欧阳伟邓文博李姣
Owner CHANGSHA KINGMED MEDICAL DIAGNOSTICS INST
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