Comprehensive biomedical experimental equipment and Internet of Things control method
By integrating constant temperature sterile anaerobic box, microplate oscillator and cutting mechanism in biomedical experimental equipment, combined with the Internet of Things module, the problem of susceptibility to contamination and heavy management of experimental data is solved, and the problem of high-quality transfer of samples between devices is achieved. Sterile environment and efficient experimental data management improve experimental efficiency and accuracy.
Patent Information
- Application Number
- CN202111003541.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Patents(China)
- Current Assignee / Owner
- Filing Date
- 2021-08-30
- Publication Date
- 2025-01-10
- Estimated Expiration
- 2041-08-30
AI Technical Summary
Traditional biomedical experimental equipment works independently, resulting in the samples being easily contaminated when transferred between equipment, and the experimental data management is heavy, making it easy to cause misoperation or recording errors.
A comprehensive biomedical experimental equipment was designed, integrating a constant temperature sterile anaerobic box, a microplate oscillator and a cutting mechanism, and the coordinated cooperation between each device is achieved through the Internet of Things module, and the temperature is monitored and regulated in real time, and the temperature is sterilized and sterilized. Sample cutting, culture and staining detection reduces sample transfer risk and manual operation burden.
The samples are sterile and constant temperature environments in each experimental link, which improves the experimental efficiency and accuracy, reduces the risk of sample contamination, and realizes real-time management of experimental data and data sharing between devices through the Internet of Things.
Smart Images

Figure CN113960322B_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of Internet of Things experimental equipment, and in particular to comprehensive biomedical experimental equipment and an Internet of Things control method. Background Art
[0002] Traditional laboratory equipment in the biomedical field is often independent of each other and works independently. Experimental samples need to be transferred between different experimental equipment in each link, which makes it easy to find contamination and damage for susceptible samples. In addition, since each device needs to independently adjust working parameters and record experimental data, a large number of experimental processes bring heavy manual operation tasks, which makes personnel easily tired and prone to misoperation or recording errors.
[0003] In order to improve the intelligence of experimental equipment, based on the rapid development of mobile Internet and Internet of Things technology, Internet of Things experimental devices came into being. Internet of Things experimental devices integrate Internet of Things modules including sensor node modules, RFID modules and communication modules to realize real-time collection, recording and transmission of experimental data to computers or mobile phones, which can greatly reduce the workload of experimental data management. At the same time, it can also realize data sharing among various experimental equipment, and then realize the synergy of mutual regulation among various devices, and set up terminals such as computers and mobile phones to monitor various experimental equipment.
[0004] In order to realize the centralized operation of bacterial separation and cultivation in the process of biomedical experiments, we provide a comprehensive integrated experimental equipment to ensure anaerobic and constant temperature environment throughout the whole process, integrate the key separation and cultivation and staining detection links, achieve the synergistic effect between equipment, and realize the mobility of gloves, which greatly improves the convenience of micro-manipulation of experimenters and meets the needs of improving experimental efficiency, ensuring sample purity, and ensuring experimental accuracy. Summary of the invention
[0005] The purpose of the present invention is to address the above problems and provide a comprehensive biomedical experimental equipment and an Internet of Things control method to meet the continuous operation of sample cutting, culture medium planting, colony culture or staining detection in the bacterial culture link in biological or medical experiments, and to adjust the working parameters of each device according to different samples and different culture media based on the Internet of Things module, while ensuring the anaerobic and suitable constant temperature environment throughout the process to avoid infection that is easy to occur during sample transfer.
[0006] To achieve the above purpose, the technical solution adopted by the present invention is: a comprehensive biomedical experimental equipment, including a constant temperature sterile anaerobic box supported by a bracket, an incubator, a microplate oscillator and a cutting mechanism are arranged in the box, a filter room and a control room are arranged next to the box, and an isolation door is arranged between the filter room and the box, characterized in that a front windshield is arranged at the front of the box, operating gloves are installed on the front windshield through a sliding plate, and an observation glass plate is arranged on the upper part of the front windshield; a glass partition is arranged in the box to isolate the incubator from the microplate oscillator, an entry and exit door is opened at the bottom of the glass partition, a slide groove is opened on the mounting platform of the box, and the slide groove passes through the entry and exit door of the glass partition, and a transfer platform is movably arranged in the slide groove; the cutting mechanism includes a cutting platform arranged on the mounting platform, and a cutting knife assembly is movably arranged on the cutting platform.
[0007] Furthermore, the observation glass plate is arranged at an angle; a hollow portion is arranged in the windshield, a long through hole is opened in the hollow portion of the windshield, and the sliding plate is movably arranged in the hollow portion and covers the through hole.
[0008] Furthermore, the box is provided with a light shield above the microplate oscillator, the top of the light shield is connected to the bottom end of the piston rod of the cylinder, and the bottom of the cylinder is installed on the top plate of the box; limiting grooves are provided on the mounting platform of the box around the microplate oscillator, and the limiting grooves shield the bottom of the light shield; ultraviolet lamps are provided on the bottom of the top plate and side walls of the box, and an ultraviolet lamp on the side wall is located between the oscillation platform of the microplate oscillator and the light shield when in the highest position.
[0009] Furthermore, a sliding door is provided at the entrance and exit of the glass partition, and the sliding door is in a normally closed state. When the transfer platform needs to pass through the entrance and exit door, the sliding door is lifted to open the entrance and exit, and a rubber sealing ring is provided at the bottom of the sliding door.
[0010] Furthermore, rollers are provided at the bottom of the transfer platform, and the rollers are limited by slide grooves. Side walls are provided on the other three sides of the transfer platform except one side close to the inner side of the box body. Clamps are symmetrically provided on the side walls on both sides of the transfer platform, and the clamps are connected to the side walls of the transfer platform by springs. A first inclined plate is provided on the top of the clamp, and a second inclined plate is provided on the end of the clamp located on the inner side of the box body.
[0011] Furthermore, a groove is provided on the upper surface of the cutting platform, and a strip groove is provided in an array inside the groove; a connecting plate is provided above the cutting platform, and both ends of the connecting plate are supported by a supporting frame, a sliding frame is provided on the connecting plate, and a cutting knife assembly is connected to the bottom of the sliding frame, and scale lines are provided on the connecting plate.
[0012] Furthermore, the cutter assembly includes a blade, the top of the blade is installed through a blade mounting plate, the top of the blade mounting plate is connected to the bottom of the connecting column through a spring, and the top of the connecting column is connected to the bottom of the sliding frame; pressing platforms are provided on both sides of the blade mounting plate.
[0013] Furthermore, a cover plate is hinged on one side of the top of the cutting platform, and an array of strip grooves corresponding to the cutting platform are arranged on the cover plate.
[0014] Furthermore, the bottom end of the cover plate is hinged to the cutting platform via a spring hinge, and a pressing block array is provided on the bottom surface of the cover plate, and each pressing block is arranged at intervals with the strip groove on the cover plate.
[0015] In addition, the present invention also discloses an Internet of Things control method for comprehensive biomedical experimental equipment, comprising the following steps:
[0016] A. Control the temperature inside the box: adjust the internal temperature of the constant temperature sterile anaerobic box according to different sample requirements to keep it in a suitable constant temperature state, and monitor it in real time through the temperature sensor and transmit the temperature data to the computer;
[0017] B. Sterilization inside the box: Before the experiment, the inside of the box is sterilized by the ultraviolet lamp on the top plate of the box to prevent the sample from being contaminated inside the box.
[0018] C. Sample cutting: For samples that need to be cut, place the materials on the cutting platform of the cutting mechanism, flip the cover plate and press the materials with the pressing block, then move the sliding frame to drive the blade to move, and cut the sample materials into the required size in cooperation with the strip groove;
[0019] D. Sample culture: The culture dish or other experimental instruments are placed and transported on the transport platform, and the samples are cultured in the incubator. The incubator is independently set and can independently control the temperature. The temperature inside the incubator is monitored by the temperature limit alarm sensor of the incubator, and the temperature data is transmitted to the control terminal such as computer or mobile phone in real time;
[0020] E. Sample staining test: For samples that need to be stained, the microplate oscillator is used to oscillate and mix the samples for staining. For samples that need to be shielded from light, the ultraviolet lamp on the side wall of the box above the microplate oscillator can be turned on for sterilization, and then the cylinder is started to drive the light shield to descend and cooperate with the limit groove to shield the microplate oscillator, and then the experimental operation can be carried out in the dark. Beneficial effects of the present invention:
[0021] In order to meet the stringent requirements of some biological or hospital experiments on the experimental environment, ensure a suitable operating environment as much as possible, and avoid the phenomenon of easy contamination during sample transportation, the present invention integrates the equipment of each key experimental link through a constant temperature box, while meeting the anaerobic and sterile experimental environment of cutting and separation and oscillation mixing or staining, and setting up an independent incubator to complete the culture link, while ensuring the risk of sterile infection in the environment during the transportation of samples, and the constant temperature control throughout the process is more conducive to the survival of the samples.
[0022] The present invention facilitates the cutting of materials in equal sizes or on-demand through the cutting mechanism. Specifically, the cutting platform is used to hold the materials, and the materials are compacted by the cover plate and the pressure block. Since the cutting platform and the cover plate are both provided with strip grooves in an array, it is convenient for the blade to cut. In addition, the blade is movably installed through the sliding frame and the connecting plate, and the scale on the connecting plate is convenient for accurate positioning. The pressing platforms on both sides of the blade mounting plate can conveniently perform micro-control operations on the blade. The spring on the upper part of the blade mounting plate not only plays a buffering and limiting role, but also plays a boosting role when operating the cutter assembly.
[0023] The present invention realizes the transport operation of samples and various experimental instruments by cooperating with the transport platform and the slideway, and the operating gloves are designed to be movable and can be translated within a certain range, which further improves the convenience of operation. Since there may be instability when the operating gloves are used for manual operation, the transport platform can also play a storage role by using the transport platform as an auxiliary transport device, which is convenient for manual operation. Clamps are arranged on both sides of the side wall of the transport platform, which can strengthen the fixing of experimental instruments such as large containers and ensure stability during the transport process, and the first inclined plate and the second inclined plate of the clamp can facilitate the clamping operation of the instrument.
[0024] The present invention can meet the operational requirements of some light-shielding experiments by setting a light shield above the microplate oscillator, and can perform light shielding when the oscillating platform oscillates. The side wall of the box is provided with an ultraviolet lamp for the microplate oscillator, and the position of the ultraviolet sterilization lamp corresponds to the position of the oscillating platform. The sterilization lamp can be turned on before and after use to strengthen the sterilization treatment of the installation space of the oscillator, thereby improving the safety and reliability of the use environment of the oscillator; at the same time, in order to improve the light shielding effect, a limiting groove is provided around the microplate oscillator, and the limiting groove shields the bottom of the light shield, which can reduce light leakage at the bottom of the light shield, which is beneficial to improving the quality of the experiment.
[0025] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a schematic diagram of the overall structure of the experimental equipment of the present invention.
[0027] Figure 2 It is a schematic diagram of the position of the through holes on the windshield of the present invention.
[0028] Figure 3 This is a schematic diagram of the operating gloves of the present invention being installed through a sliding plate.
[0029] Figure 4 It is a schematic diagram of the position of the sliding plate of the present invention.
[0030] Figure 5 It is a schematic diagram of the position of the glass partition in the box of the present invention.
[0031] Figure 6 It is a schematic diagram of the interior of the box of the present invention.
[0032] Figure 7 It is a schematic structural diagram of the cutting mechanism of the present invention.
[0033] Figure 8 It is a schematic structural diagram of the cutter assembly of the present invention.
[0034] Fig. 9 The figure is a schematic diagram of installing the sunshade of the present invention.
[0035] Fig.10 It is a schematic structural diagram of the transfer platform of the present invention.
[0036] The text labels in the figure are as follows: 1. universal wheel; 2. bracket; 3. filter chamber; 4. control chamber; 5. observation glass plate; 6. box body; 7. sealing door; 8. operating gloves; 9. sliding plate; 10. front windshield; 11. through hole; 12. hollow part; 13. cutting mechanism; 14. incubator; 15. glass partition; 16. light shield; 17. ultraviolet lamp; 18. cylinder; 19. oscillation platform; 20. microplate oscillator; 21 , access door; 22, sliding door; 23, transfer platform; 24, slide; 25, cutting platform; 26, installation platform; 27, support frame; 28, connecting plate; 29, cutter assembly; 30, groove; 31, strip groove; 32, cover plate; 33, pressure block; 34, sliding frame; 35, connecting column; 36, spring; 37, blade mounting plate; 38, blade; 39, limit groove; 40, clamp; 41, first inclined plate; 42, second inclined plate. DETAILED DESCRIPTION
[0037] The present invention is further explained below by way of examples.
[0038] A comprehensive biomedical experimental equipment comprises a constant temperature sterile anaerobic box 6 supported by a bracket 2, an incubator 14, a microplate oscillator 20 and a cutting mechanism 13 are arranged in the box 6, a filter chamber 3 and a control chamber 4 are arranged next to the box 6, and an isolation door is arranged between the filter chamber 3 and the box 6, characterized in that a front windshield 10 is arranged at the front of the box 6, an operating glove 8 is installed on the front windshield 10 through a sliding plate 9, and an observation glass plate 5 is arranged on the upper part of the front windshield 10; a glass partition 15 is arranged in the box 6 to isolate the incubator 14 from the microplate oscillator 20, an access door 21 is opened at the bottom of the glass partition 15, a slide 24 is opened on the installation platform 26 of the box 6, and the slide 24 passes through the access door 21 of the glass partition 15, and a transfer platform 23 is movably arranged in the slide 24; the cutting mechanism 13 comprises a cutting platform 25 arranged on the installation platform 26, and a cutting knife assembly 29 is movably arranged on the cutting platform 25.
[0039] Preferably, please refer to Figure 2 , 3 As shown in 4, the observation glass plate 5 is arranged at an angle; a hollow portion 12 is arranged in the windshield 10, and a long through hole 11 is opened in the hollow portion 12 of the windshield 10, and the sliding plate 9 is movably arranged in the hollow portion 12 and covers the through hole 11.
[0040] Preferably, please refer to Figure 5 , 6 As shown in Figures 9 and 10, the box 6 is provided with a light shield 16 above the microplate oscillator 20, the top of the light shield 16 is connected to the bottom end of the piston rod of the cylinder 18, and the bottom of the cylinder 18 is installed on the top plate of the box 6; the installation platform 26 of the box 6 is provided with a limiting groove 39 around the microplate oscillator 20, and the limiting groove 39 shields the bottom of the light shield 16; the bottom of the top plate and the side wall of the box 6 are provided with an ultraviolet lamp 17, and one ultraviolet lamp 17 on the side wall is located between the oscillation platform 19 of the microplate oscillator 20 and the light shield 16 at the highest position. Before the microplate oscillator 20 is used, the ultraviolet lamp 17 can be turned on to strengthen the sterilization treatment of the installation space of the oscillator, thereby improving the safety and reliability of the use environment of the oscillator. At the same time, the setting of the limiting groove 39 and the light shield 16 can improve the shading effect.
[0041] Preferably, please refer to Figure 6As shown, a sliding door 22 is provided at the entrance door 21 of the glass partition 15, and the sliding door 22 is in a normally closed state. When the transfer platform 23 needs to pass through the entrance door 21, the sliding door 22 is lifted to open the entrance door 21, and a rubber sealing ring is provided at the bottom of the sliding door 22. The glass partition 15 can partition the interior of the box 6 and separate the incubator 14 from the microplate oscillator 20, which can facilitate sterilization or temperature control in different zones, and when the microplate oscillator 20 is not needed to work, the energy consumption of the zone can be reduced.
[0042] Preferably, please refer to Figure 6 , 10 As shown, the bottom of the transfer platform 23 is provided with rollers, which are limited by the slide groove 24. The transfer platform 23 is provided with side walls on the other three sides except one side close to the inner side of the box body 6. Clamps 40 are symmetrically provided on the side walls on both sides of the transfer platform 23. The clamps 40 are connected to the side walls of the transfer platform 23 by springs 36. A first inclined plate 41 is provided on the top of the clamps 40, and a second inclined plate 42 is provided at one end of the clamps 40 located on the inner side of the box body 6. The clamps 40 can strengthen the fixing of experimental instruments such as containers with larger volumes to ensure stability during the transfer process, and the first inclined plate 41 and the second inclined plate 42 of the clamps 40 can facilitate the clamping of instruments from the top of the transfer platform 23 to the bottom or from the front to the back.
[0043] Preferably, please refer to Figure 6 , 7 As shown in Figures 8 and 9, the upper surface of the cutting platform 25 is provided with a groove 30, and strip grooves 31 are arranged in an array in the groove 30; a connecting plate 28 is arranged above the cutting platform 25, and the two ends of the connecting plate 28 are supported by a support frame 27, and a sliding frame 34 is sleeved on the connecting plate 28, and a cutter assembly 29 is connected to the bottom of the sliding frame 34, and a scale line is arranged on the connecting plate 28. The cutter assembly 29 includes a blade 38, and the top of the blade 38 is installed through a blade mounting plate 37, and the top of the blade mounting plate 37 is connected to the bottom of the connecting column 35 through a spring 36, and the top of the connecting column 35 is connected to the bottom of the sliding frame 34; pressing platforms are arranged on both sides of the blade mounting plate 37. A cover plate 32 is hinged on one side of the top of the cutting platform 25, and a strip groove 31 corresponding to the cutting platform 25 is arranged in an array on the cover plate 32. The bottom end of the cover plate 32 is hinged to the cutting platform 25 via a spring hinge. The bottom surface of the cover plate 32 is provided with a pressing block 33 in an array. Each pressing block 33 is arranged at intervals with the strip groove 31 on the cover plate 32 .
[0044] In addition, the present invention also discloses an Internet of Things control method for comprehensive biomedical experimental equipment, comprising the following steps:
[0045] A. Control the temperature inside the box 6: adjust the internal temperature of the constant temperature sterile anaerobic box 6 according to different sample requirements to keep it in a suitable constant temperature state, and monitor it in real time through the temperature sensor and transmit the temperature data to the computer;
[0046] B. Sterilization inside the box 6: Before the experiment, the inside of the box 6 is sterilized by the ultraviolet lamp 17 on the top plate of the box 6 to prevent the sample from being contaminated inside the box 6.
[0047] C. Sample cutting: For the sample to be cut, the cutting platform 25 of the cutting mechanism 13 is used to hold the material, the cover plate 32 is turned over, the material is pressed by the pressing block 33, and the movable sliding frame 34 drives the blade 38 to move, and the sample material is cut into the required size in cooperation with the strip groove 31;
[0048] D. Sample culture: The culture dish or other experimental instruments are placed and transported on the transport platform 23, and the samples are cultured in the incubator 14. The incubator 14 is independently set and can independently control the temperature. The temperature inside the incubator 14 is monitored by the temperature limit alarm sensor of the incubator 14;
[0049] E. Sample staining test: For samples that need to be stained, the microplate oscillator 20 is used to oscillate and mix the samples for staining. For samples that need to be shielded from light, the ultraviolet lamp 17 located above the microplate oscillator 20 on the side wall of the box 6 can be turned on for sterilization, and then the cylinder 18 can be started to drive the light shielding cover 16 to descend and cooperate with the limit groove 39 to shield the microplate oscillator 20, and then the experimental operation can be carried out in the dark.
[0050] It should be noted that, in this article, the terms "comprises", "includes" or any other variations thereof are intended to cover non-exclusive inclusion, so that a process, method, article or apparatus that includes a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or apparatus.
[0051] The principles and implementation methods of the present invention are explained herein. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. At the same time, for those skilled in the art, according to the idea of the present invention, there will be changes in the specific implementation methods and application scope. In summary, the content of this specification should not be understood as limiting the present invention.
Claims
1. A biomedical comprehensive experimental device, comprising a constant temperature sterile anaerobic box (6) supported by a support (2), wherein an incubator (14), a microplate oscillator (20) and a cutting mechanism (13) are arranged in the box (6), a filter chamber (3) and a control chamber (4) are arranged next to the box (6), and an isolation door is arranged between the filter chamber (3) and the box (6), characterized in that: The front part of the box (6) is provided with a front windshield (10), an operating glove (8) is installed on the front windshield (10) through a sliding plate (9), and an observation glass plate (5) is arranged on the top of the front windshield (10); a glass partition (15) is arranged in the box (6) to isolate the incubator (14) from the microplate oscillator (20), an access door (21) is provided at the bottom of the glass partition (15), a slide groove (24) is provided on the mounting platform (26) of the box (6), and the slide groove (24) passes through the access door (21) of the glass partition (15), and a transfer platform (23) is movably arranged in the slide groove (24); the cutting mechanism (13) comprises a cutting platform (25) arranged on the mounting platform (26), and a cutting knife assembly (29) is movably arranged on the cutting platform (25); The box (6) is provided with a light shield (16) located above the microplate oscillator (20), the top of the light shield (16) is connected to the bottom end of the piston rod of the cylinder (18), and the bottom of the cylinder (18) is installed on the top plate of the box (6); the bottom of the top plate and the side wall of the box (6) are both provided with ultraviolet lamps (17); A cover plate (32) is hingedly connected to one side of the top of the cutting platform (25), and an array of strip grooves (31) corresponding to the cutting platform (25) are provided on the cover plate (32). A pressing block (33) is arranged in an array on the bottom surface of the cover plate (32), and each pressing block (33) is arranged at intervals from the strip groove (31) on the cover plate (32).
2. The comprehensive biomedical experimental equipment according to claim 1, characterized in that: The observation glass plate (5) is arranged at an angle; a hollow portion (12) is arranged in the windshield (10); a long through hole (11) is opened in the windshield (10) at the hollow portion (12); and the sliding plate (9) is movably arranged in the hollow portion (12) and covers the through hole (11).
3. The comprehensive biomedical experimental equipment according to claim 1, characterized in that: The mounting platform (26) of the box (6) is provided with limiting grooves (39) around the microplate oscillator (20), and the limiting grooves (39) shield the bottom of the light shield (16); an ultraviolet lamp (17) on the side wall is located between the oscillation platform (19) of the microplate oscillator (20) and the light shield (16) at the highest position.
4. The comprehensive biomedical experimental equipment according to claim 1, characterized in that: A sliding door (22) is provided at the entrance door (21) of the glass partition (15), and the sliding door (22) is in a normally closed state. When the transfer platform (23) needs to pass through the entrance door (21), the sliding door (22) is lifted to open the entrance door (21), and a rubber sealing ring is provided at the bottom of the sliding door (22).
5. The comprehensive biomedical experimental equipment according to claim 1, characterized in that: The bottom of the transfer platform (23) is provided with a roller, which is limited by a slide groove (24). The transfer platform (23) is provided with side walls on three sides except for one side close to the inner side of the box body (6). Clamps (40) are symmetrically provided on the side walls on both sides of the transfer platform (23). The clamps (40) and the side walls of the transfer platform (23) are connected by springs (36). A first inclined plate (41) is provided on the top of the clamps (40), and a second inclined plate (42) is provided on one end of the clamps (40) located on the inner side of the box body (6).
6. The comprehensive biomedical experimental equipment according to claim 1, characterized in that: The cutting platform (25) has an upper surface provided with a groove (30), and an array of strip grooves (31) is provided in the groove (30); a connecting plate (28) is provided above the cutting platform (25), and both ends of the connecting plate (28) are supported by support frames (27); a sliding frame (34) is sleeved on the connecting plate (28), and a cutting knife assembly (29) is connected to the bottom of the sliding frame (34); and scale lines are provided on the connecting plate (28).
7. The biomedical comprehensive experimental equipment according to claim 6, characterized in that: The cutter assembly (29) comprises a blade (38), the top of the blade (38) being mounted via a blade mounting plate (37), the top of the blade mounting plate (37) being connected to the bottom of a connecting column (35) via a spring (36), and the top of the connecting column (35) being connected to the bottom of a sliding frame (34); pressing platforms are provided on both sides of the blade mounting plate (37).
8. The comprehensive biomedical experimental equipment according to claim 1, characterized in that: The bottom end of the cover plate (32) is hinged to the cutting platform (25) via a spring hinge.
9. The Internet of Things control method for biomedical comprehensive experimental equipment according to claim 1, comprising the following steps: A. Controlling the temperature inside the box (6): adjusting the internal temperature of the constant temperature sterile anaerobic box (6) according to different sample requirements to maintain a suitable constant temperature state, and monitoring the temperature in real time through a temperature sensor and transmitting the temperature data to a computer; B. Sterilization inside the box (6): Before the experiment, the inside of the box (6) is sterilized by the ultraviolet lamp (17) on the top plate of the box (6) to prevent the sample from being contaminated inside the box (6); C. Sample cutting: For the sample to be cut, the cutting platform (25) of the cutting mechanism (13) is used to hold the material, the cover plate (32) is turned over, and the material is pressed by the pressing block (33), and then the movable sliding frame (34) drives the blade (38) to move, and the sample material is cut into the required size in cooperation with the strip groove (31); D. Sample culture: a culture dish or other experimental instrument is placed and transported on a transport platform (23), and the sample is cultured in an incubator (14). The incubator (14) is independently set and can independently control the temperature. The temperature inside the incubator (14) is monitored by a temperature limit alarm sensor of the incubator (14), and the temperature data is transmitted in real time to a control terminal such as a computer or a mobile phone; E. Sample staining test: The samples to be stained are oscillated and mixed by a microplate oscillator (20) for staining. For samples to be tested in the dark, the ultraviolet lamp (17) located above the microplate oscillator (20) on the side wall of the box (6) can be turned on for sterilization. Then, the cylinder (18) is started to drive the light shield (16) to descend and cooperate with the limit groove (39) to shield the microplate oscillator (20). Then, the experimental operation can be carried out in the dark.
Citation Information
Patent Citations
Self-contained biological assay apparatus, methods, and applications
CN102906573A
Cell culture device having culture medium replacement function
CN104169413A