A cell separation and extraction processing device

Abstract

The present invention relates to the field of bioengineering technology, and in particular to a cell separation, extraction and processing device, comprising a bottom plate, a rotating part, a constant temperature part and a clamping part, wherein the upper end of the bottom plate is provided with a rotating part, the middle part of the upper end of the rotating part is provided with a constant temperature part, and the upper end of the rotating part is provided with a plurality of clamping parts evenly distributed in the circumferential direction. The present invention can solve the problem that when separating cells in biological tissues, trypsin is mixed with the tissue and then subjected to rotational centrifugation, and this method is easy to separate and process the cells of the tissue, and the mixed solution after centrifugation needs a lot of time to precipitate, thereby increasing the time for cell separation. The suction mechanism used in the cell separation, extraction and processing device provided by the present invention can block the test tube when the cells are centrifuged, thereby preventing bacteria in the air from contaminating the cells, ensuring that the separated cells remain active, and improving the success rate of cell separation.

Description

Technical Field

[0001] The invention relates to the technical field of bioengineering, and in particular to a cell separation, extraction and processing device. Background Art

[0002] The purpose of cell separation is to obtain individual cells, so as to facilitate the study of the nucleus, mitochondria, Golgi apparatus, lysosomes and microbodies in the cell. Cell separation includes mechanical dispersion and digestion separation. Existing cell separation usually uses the trypsin dispersion technology in the digestion separation method.

[0003] When separating cells in biological tissues, trypsin is usually mixed with the tissue and then subjected to rotary centrifugation. This method makes it easy to separate the cells of the tissue. The mixed solution after centrifugation needs a lot of time to precipitate, and then the liquid and cells are separated, which increases the time for cell separation and reduces the efficiency of cell separation.

[0004] In the existing cell separation method, after the tissue is centrifuged and precipitated, the separated liquid needs to be sucked out. Due to the small diameter of the test tube, a larger suction device cannot enter the test tube, and the amount of liquid sucked out needs to be precisely controlled. A larger suction device is likely to suck out cells and liquid together. Therefore, a dropper is usually used to suck out the liquid in the test tube. This method requires repeated operations many times, making the cell separation operation cumbersome. Summary of the invention

[0005] Technical problem to be solved: The cell separation, extraction and processing device provided by the present invention can solve the problems existing in cell separation and extraction of biological tissues mentioned above.

[0006] Technical solution: In order to achieve the above-mentioned purpose, the present invention adopts the following technical solution, a cell separation and extraction processing device, including a base plate, a rotating part, a constant temperature part and a clamping part, the upper end of the base plate is equipped with a rotating part, the middle part of the upper end of the rotating part is equipped with a constant temperature part, and the upper end of the rotating part is equipped with multiple clamping parts evenly distributed in the circumference.

[0007] The camming mechanism is a kind of structured rotation of the camming rod and the camshaft is a kind of structured rotation of the camshafts, and the camshafts are used to move the camshafts in a rotationally-aligned manner.

[0008] The suction mechanism includes a circular ring plate placed above the placing sleeve, a sliding tube slidingly penetrates the middle of the circular ring plate, a sealing plate is installed at the upper end of the sliding tube, a return spring is installed between the lower end of the sealing plate and the circular ring plate, a water inlet through hole is opened in the lower wall of the sliding tube, and a baffle net is installed on the wall of the water inlet through hole, a rotating rod is rotatably connected in the middle of the upper end of the lower wall of the sliding tube, the upper end of the rotating rod penetrates and rotatably cooperates with the sealing plate, a fan-shaped plate is installed near the lower end of the outer surface of the rotating rod, the fan-shaped plate rotates and cooperates with the lower wall of the sliding tube, a linkage rod slides through the lower wall of the sliding tube up and down, the linkage rod and the sealing plate slide through, the linkage rod has a U-shaped structure, a dense hole plate is installed at the lower end of the linkage rod side rod inside the sliding tube, and an auxiliary plate is installed at the lower end of the linkage rod side rod outside the sliding tube, an adjusting screw is threadedly connected to the middle of the auxiliary plate, the lower end of the adjusting screw is rotatably connected to the circular ring plate, and a rubber ring is provided near the middle of the outer surface of the sliding tube.

[0009] As a preferred technical solution of the present invention, the clamping mechanism includes a clamping ring, a clamping ring is installed on the upper side of one end of the support rod close to the circumferential distribution center, a plurality of circumferentially evenly distributed clamping rods are slidably connected to the inner wall of the clamping ring, a clamping block is installed at one end of the clamping rod away from the inner wall of the clamping ring, and a clamping spring is installed between the clamping block and the inner wall of the clamping ring.

[0010] As a preferred technical solution of the present invention, the rotating part includes a rotating motor, the rotating motor is installed on the upper end of the base plate, a rotating plate is installed on the output shaft of the rotating motor, a plurality of circumferentially evenly distributed supporting circular blocks are installed on the upper end of the rotating plate, and a semicircular groove is opened on the upper end of the supporting circular block.

[0011] As a preferred technical solution of the present invention, the constant temperature component includes a cylindrical rod, a cylindrical rod is installed in the middle of the upper end of the rotating plate, a plurality of circumferentially evenly distributed connecting plates are installed on the outer surface of the cylindrical rod, and a constant temperature sleeve is installed at the end of the connecting plate away from the cylindrical rod.

[0012] As a preferred technical solution of the present invention, an inclined surface is arranged at the upper end of the clamping block, and a rubber pad is laid on the clamping block.

[0013] As a preferred technical solution of the present invention, a protective cover is plugged into the upper end of the bottom plate, the protective cover is made of glass, and a handle is provided on the upper end of the protective cover.

[0014] As a preferred technical solution of the present invention, glass plates are symmetrically installed on the side walls of the thermostatic jacket, scale bars are arranged on the side walls of the thermostatic jacket near the glass plates, and a thermostatic ring is arranged on the inner wall of the thermostatic jacket.

[0015] As a preferred technical solution of the present invention, a sealing ring is sleeved on the side wall of the dense hole plate.

[0016] Beneficial effects: 1. The suction mechanism used in the cell separation, extraction and processing device provided by the present invention can block the test tube during cell centrifugation, thereby preventing bacteria in the air from contaminating the cells, ensuring that the separated cells remain active, and improving the success rate of cell separation.

[0017] 2. The suction mechanism used in the cell separation, extraction and processing device provided by the present invention can separate cells from liquid when the two are separated, avoiding the loss of a large number of cells when the liquid is discharged, thereby ensuring the cell content after cell separation, avoiding cell waste, and reducing the time of cell sedimentation, thereby improving the efficiency of cell separation.

[0018] 3. The suction mechanism used in the cell separation and extraction processing device provided by the present invention can separate cells from liquid in one operation, thereby reducing the operation steps of cell separation.

[0019] 4. The constant temperature element used in the cell separation, extraction and processing device provided by the present invention can maintain a constant temperature during the cell separation process, thereby ensuring the degree of cell separation and reducing the phenomenon of a large number of incomplete separations of cell tissues. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention is further described below in conjunction with the accompanying drawings and embodiments;

[0021] Figure 1 is a schematic diagram of a three-dimensional structure from a first viewing angle of the present invention;

[0022] Figure 2 It is a schematic diagram of the three-dimensional structure of the present invention without the protective cover;

[0023] Figure 3 It is a front view of the present invention;

[0024] Figure 4 The present invention Figure 3 Sectional view of AA in the middle;

[0025] Figure 5 The present invention Figure 4 Enlarged view of G in the middle;

[0026] Figure 6 The present invention Figure 4 The enlarged view of the H in the middle;

[0027] Figure 7 It is a schematic cross-sectional view of the structure of the placement sleeve, the positioning screw and the suction mechanism of the present invention.

[0028] In the figure: 1, bottom plate; 2, rotating part; 21, rotating motor; 22, rotating plate; 23, supporting round block; 3, constant temperature part; 31, cylindrical rod; 32, connecting plate; 33, constant temperature sleeve; 4, clamping part; 41, supporting rod; 42, clamping mechanism; 421, clamping ring; 422, clamping rod; 423, clamping block; 424, clamping spring; 43, lifting rod; 44, lifting plate; 45, square plate; 46, positioning rod; 47, clamping rod; 48, placing sleeve; 49, positioning screw; 40, suction mechanism; 401, circular ring plate; 402, sliding tube; 403, sealing plate; 404, reset spring; 405, rotating rod; 406, fan-shaped plate; 407, baffle net; 408, linkage rod; 409, dense hole plate; 410, adjusting screw. DETAILED DESCRIPTION

[0029] The embodiments of the present invention are described in detail below with reference to the accompanying drawings, but the present invention can be implemented in many different ways as defined and covered by the claims.

[0030] See also Figure 1-Figure 3 A cell separation, extraction and processing device comprises a base plate 1, a rotating part 2, a constant temperature part 3 and a clamping part 4. The rotating part 2 is installed on the upper end of the base plate 1, the constant temperature part 3 is installed in the middle of the upper end of the rotating part 2, and a plurality of clamping parts 4 evenly distributed in the circumferential direction are installed on the upper end of the rotating part 2. A protective cover is plugged into the upper end of the base plate 1, the protective cover is made of glass, and a handle is arranged on the upper end of the protective cover.

[0031] See also Figure 2 , Figure 4 , Figure 5 and Figure 6The clamping member 4 includes a plurality of support rods 41 evenly distributed in the circumferential direction and installed on the rotating member 2. A clamping mechanism 42 is installed on the upper side of one end of the support rod 41 close to the circumferential distribution center. A cylindrical groove is provided at the upper end of the support rod 41. A lifting rod 43 is slidably connected inside the cylindrical groove. A sliding through hole is provided on the side of the cylindrical groove wall away from the clamping mechanism 42. A lifting plate 44 is installed at the lower end of the lifting rod 43. The lifting plate 44 is slidably matched with the sliding through hole. A square plate 45 is arranged above the lifting plate 44. The square plate 45 is installed on the support rod 41. A positioning rod 46 is provided with a plurality of evenly distributed rectangular through holes. The lower end of the positioning rod 46 slides through the lifting plate 44 for cooperation. A clamping rod 47 slides through the lifting plate 44. The clamping rod 47 slides with the rectangular through hole. The lifting rod 43 is symmetrically provided with threaded holes near the upper end. A placement sleeve 48 is rotatably connected to the upper end of the lifting rod 43. A positioning screw 49 is threadedly connected to the side wall of the placement sleeve 48. The positioning screw 49 is threadedly cooperated with the threaded hole. A suction mechanism 40 is inserted into the placement sleeve 48. An anti-slip sleeve is provided on the clamping rod 47.

[0032] During the specific operation, multiple test tubes containing trypsin and cell tissues are manually passed through multiple clamping mechanisms 42 and placed on the rotating member 2. The clamping mechanisms 42 clamp and limit the test tubes. Then, according to the height of the test tubes, the lifting plate 44 is manually extracted upward, and the lifting plate 44 drives the lifting rod 43 to rise. The lifting rod 43 drives the placement sleeve 48 to move to the same height at the upper end of the test tube, and then the clamping rod 47 passes through the lifting plate 44 and is inserted into the rectangular through hole on the positioning rod 46, so as to support and limit the placement sleeve 48, and the placement sleeve is manually rotated. 48, the placement sleeve 48 moves to the upper end of the test tube and limits the test tube, and then the suction mechanism 40 is manually passed through the placement sleeve 48 and inserted into the test tube, thereby limiting the cell tissue in the test tube to prevent the cells from spilling during the subsequent rotation and centrifugation. The protective cover is manually placed on the bottom plate 1 through the handle, and the rotating part 2 drives the cell tissue for centrifugal treatment. After the cell tissue is rotated and centrifuged, the suction mechanism 40 extracts and discharges the liquid generated by the rotation, and then the test tube is manually taken out of the clamping mechanism 42, and the cell extraction is completed.

[0033] See also Figure 7The suction mechanism 40 includes a circular plate 401 placed above the placement sleeve 48, a sliding tube 402 is slidably penetrated in the middle of the circular plate 401, a sealing plate 403 is installed on the upper end of the sliding tube 402, a return spring 404 is installed between the lower end of the sealing plate 403 and the circular plate 401, a water inlet through hole is opened on the lower wall of the sliding tube 402, a baffle net 407 is installed on the wall of the water inlet through hole, a rotating rod 405 is rotatably connected to the middle of the upper end of the lower wall of the sliding tube 402, the upper end of the rotating rod 405 penetrates and rotates with the sealing plate 403, a fan-shaped plate 406 is installed near the lower end of the outer surface of the rotating rod 405, and the fan-shaped plate 406 is installed. 6 is rotatably matched with the lower wall of the sliding tube 402, and a linkage rod 408 is penetrated through the lower wall of the sliding tube 402 for sliding up and down movement, and the linkage rod 408 slides through the sealing plate 403, and the linkage rod 408 is U-shaped. A dense hole plate 409 is installed at the lower end of the side rod of the linkage rod 408 inside the sliding tube 402, and a sealing ring is sleeved on the side wall of the dense hole plate 409. An auxiliary plate is installed at the lower end of the side rod of the linkage rod 408 outside the sliding tube 402, and an adjusting screw 410 is threadedly connected to the middle of the auxiliary plate. The lower end of the adjusting screw 410 is rotatably connected to the circular ring plate 401, and a rubber ring is provided near the middle of the outer surface of the sliding tube 402.

[0034] During the specific operation, the sliding tube 402 is manually inserted into the test tube, the annular plate 401 is placed on the placement sleeve 48, and the rubber ring on the outer surface of the sliding tube 402 seals the test tube to prevent external bacteria from affecting the cells. After the cell centrifugation is completed, the adjusting screw 410 is manually rotated, and the adjusting screw 410 drives the dense hole plate 409 to descend through the linkage rod 408. The pore size on the dense hole plate 409 is smaller than the diameter of the cell, so that the cells suspended in the liquid and the cells adhering to the wall of the test tube are pressed downward to separate the cells from the liquid. Then, the adjusting screw 410 is manually rotated to drive the dense hole plate 409 to descend through the linkage rod 408. The pore size on the dense hole plate 409 is smaller than the diameter of the cell. The rotating rod 405 is rotated, and the rotating rod 405 drives the fan-shaped plate 406 to move out of the water inlet hole, and then the sliding tube 402 is pressed downward, and the sliding tube 402 flows the liquid into the sliding tube 402 through the baffle net 407. When the sliding tube 402 moves to the upper end of the dense hole plate 409, the rotating rod 405 is manually rotated, and the rotating rod 405 drives the fan-shaped plate 406 to move above the water inlet hole, thereby sealing the water inlet hole, and the sliding tube 402 filled with liquid is manually taken out of the test tube, thereby separating the liquid in the test tube to facilitate cell extraction.

[0035] See also Figure 5The clamping mechanism 42 includes a clamping ring 421, and a clamping ring 421 is installed on the upper side of one end of the support rod 41 close to the circumferential distribution center. A plurality of circumferentially evenly distributed clamping rods 422 are slidably connected to the inner wall of the clamping ring 421, and a clamping block 423 is installed at one end of the clamping rod 422 away from the inner wall of the clamping ring 421. A clamping spring 424 is installed between the clamping block 423 and the inner wall of the clamping ring 421, and an inclined surface is provided on the upper end of the clamping block 423, and a rubber pad is laid on the clamping block 423. During specific work, the test tube is manually inserted into the clamping ring 421, and the clamping block 423 clamps and limits the test tube through the clamping spring 424. The inclined surface on the clamping block 423 facilitates the entry of the test tube.

[0036] See also Figure 4 The rotating member 2 includes a rotating motor 21. The rotating motor 21 is installed on the upper end of the bottom plate 1. A rotating plate 22 is installed on the output shaft of the rotating motor 21. A plurality of circumferentially evenly distributed supporting round blocks 23 are installed on the upper end of the rotating plate 22. A semicircular groove is opened on the upper end of the supporting round block 23. During specific operation, the test tube is manually placed on the supporting round block 23, and then the rotating motor 21 is started. The rotating motor 21 drives the test tube on the supporting round block 23 to rotate through the rotating plate 22, thereby realizing the function of cell tissue centrifugation.

[0037] See also Figure 4 The thermostatic component 3 includes a cylindrical rod 31, a cylindrical rod 31 is installed in the middle of the upper end of the rotating plate 22, a plurality of circumferentially evenly distributed connecting plates 32 are installed on the outer surface of the cylindrical rod 31, a thermostatic sleeve 33 is installed at one end of the connecting plate 32 away from the cylindrical rod 31, glass plates are symmetrically installed on the side wall of the thermostatic sleeve 33, scale bars are arranged near the glass plates on the side wall of the thermostatic sleeve 33, and a thermostatic ring is arranged on the inner wall of the thermostatic sleeve 33. During specific operation, the test tube is manually passed through the thermostatic sleeve 33, and the thermostatic ring in the thermostatic sleeve 33 is an existing heating ring, so as to ensure that the cell tissue is centrifuged and separated at a constant temperature, the glass plate is convenient for observing the inside of the test tube, and the scale bar is convenient for observing the content in the test tube.

[0038] During separation: S1: Multiple test tubes containing trypsin and cell tissues are manually passed through multiple clamping rings 421 and constant temperature sleeves 33 in turn, and placed on the supporting round block 23. Then, according to the height of the test tubes, the lifting plate 44 is manually extracted upwards, and the lifting plate 44 drives the placement sleeve 48 to move to the same height of the upper end of the test tube through the lifting rod 43, and then the placement sleeve 48 is supported and limited by the clamping rod 47. The placement sleeve 48 is manually rotated, and the placement sleeve 48 moves to the upper end of the test tube and limits the test tube, and the constant temperature ring in the constant temperature sleeve 33 heats the test tube.

[0039] S2: Manually insert the sliding tube 402 into the test tube, place the annular plate 401 on the placement sleeve 48, and then place the protective cover on the bottom plate 1 through the handle, and then start the rotating motor 21. The rotating motor 21 drives the test tube on the supporting round block 23 to rotate through the rotating plate 22, thereby realizing the function of cell tissue centrifugation.

[0040] S3: The protective cover is manually removed from the bottom plate 1 through the handle, and then the adjusting screw 410 is manually rotated. The adjusting screw 410 drives the dense hole plate 409 to descend through the linkage rod 408. The dense hole plate 409 separates the cells suspended in the liquid and the cells adhering to the wall of the test tube from the liquid. The rotating rod 405 is manually rotated to open the water inlet hole, and then the sliding tube 402 is pressed downward. The sliding tube 402 flows the liquid into the sliding tube 402 through the baffle net 407. When the sliding tube 402 moves to the upper end of the dense hole plate 409, the rotating rod 405 is manually rotated to drive the fan plate 406 to close the water inlet hole. The sliding tube 402 containing the liquid is manually taken out of the test tube, thereby separating the liquid in the test tube to facilitate the extraction of cells.

[0041] S4: The test tube is manually taken out from the clamping ring 421, and the cell extraction is completed.

[0042] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A cell separation and extraction processing device, comprising a bottom plate (1), a rotating member (2), a constant temperature member (3) and a clamping member (4), characterized in that: A rotating member (2) is installed at the upper end of the bottom plate (1), a constant temperature member (3) is installed in the middle of the upper end of the rotating member (2), and a plurality of circumferentially evenly distributed clamping members (4) are installed at the upper end of the rotating member (2); wherein: The clamping member (4) comprises a plurality of support rods (41) uniformly distributed in the circumferential direction and mounted on the rotating member (2); a clamping mechanism (42) is mounted on the upper side of one end of the support rod (41) close to the circumferential distribution center; a cylindrical groove is provided at the upper end of the support rod (41); a lifting rod (43) is slidably connected inside the cylindrical groove; a sliding through hole is provided on the side of the cylindrical groove wall away from the clamping mechanism (42); a lifting plate (44) is mounted at the lower end of the lifting rod (43); the lifting plate (44) is slidably matched with the sliding through hole; a square plate (45) is arranged above the lifting plate (44); the square plate (45) is mounted on the support rod (41); a A positioning rod (46) is provided with a plurality of evenly distributed rectangular through holes. The lower end of the positioning rod (46) is slidably penetrated by the lifting plate (44). A clamping rod (47) is slidably penetrated by the lifting plate (44). The clamping rod (47) and the rectangular through hole are slidably cooperated with each other. A threaded hole is symmetrically provided near the upper end of the lifting rod (43). A placement sleeve (48) is rotatably connected to the upper end of the lifting rod (43). A positioning screw (49) is threadedly connected to the side wall of the placement sleeve (48). The positioning screw (49) is threadedly cooperated with the threaded hole. A suction mechanism (40) is inserted into the interior of the placement sleeve (48). An anti-slip sleeve is provided on the clamping rod (47). The suction mechanism (40) comprises a circular plate (401) placed above the placement sleeve (48), a sliding tube (402) slidingly penetrates the middle of the circular plate (401), a sealing plate (403) is installed at the upper end of the sliding tube (402), a return spring (404) is installed between the lower end of the sealing plate (403) and the circular plate (401), a water inlet through hole is opened on the lower wall of the sliding tube (402), a baffle net (407) is installed on the wall of the water inlet through hole, a rotating rod (405) is rotatably connected to the middle of the upper end of the lower wall of the sliding tube (402), the upper end of the rotating rod (405) penetrates and rotatably cooperates with the sealing plate (403), and a fan-shaped plate (404) is installed on the outer surface of the rotating rod (405) near the lower end. 406), the fan-shaped plate (406) is rotatably matched with the lower wall of the sliding tube (402), the lower wall of the sliding tube (402) is slidably penetrated by a linkage rod (408), the linkage rod (408) and the sealing plate (403) are slidably penetrated, the linkage rod (408) is U-shaped, the lower end of the side rod of the linkage rod (408) located inside the sliding tube (402) is installed with a dense hole plate (409), the lower end of the side rod of the linkage rod (408) located outside the sliding tube (402) is installed with an auxiliary plate, the middle part of the auxiliary plate is threadedly connected with an adjusting screw (410), the lower end of the adjusting screw (410) is rotatably connected with the circular ring plate (401), and a rubber ring is arranged near the middle of the outer surface of the sliding tube (402).

2. A cell separation, extraction and processing device according to claim 1, characterized in that: The clamping mechanism (42) comprises a clamping ring (421), a clamping ring (421) is installed on the upper side of one end of the support rod (41) close to the circumferential distribution center, a plurality of circumferentially evenly distributed clamping rods (422) are slidably connected to the inner wall of the clamping ring (421), a clamping block (423) is installed at one end of the clamping rod (422) away from the inner wall of the clamping ring (421), and a clamping spring (424) is installed between the clamping block (423) and the inner wall of the clamping ring (421).

3. A cell separation, extraction and processing device according to claim 1, characterized in that: The rotating member (2) comprises a rotating motor (21), the rotating motor (21) is mounted on the upper end of the bottom plate (1), a rotating plate (22) is mounted on the output shaft of the rotating motor (21), a plurality of circumferentially evenly distributed supporting round blocks (23) are mounted on the upper end of the rotating plate (22), and a semicircular groove is formed on the upper end of the supporting round blocks (23).

4. A cell separation, extraction and processing device according to claim 3, characterized in that: The thermostatic component (3) comprises a cylindrical rod (31), the cylindrical rod (31) being mounted in the middle of the upper end of the rotating plate (22), a plurality of circumferentially evenly distributed connecting plates (32) being mounted on the outer surface of the cylindrical rod (31), and a thermostatic sleeve (33) being mounted at one end of the connecting plate (32) away from the cylindrical rod (31).

5. A cell separation, extraction and processing device according to claim 2, characterized in that: The upper end of the clamping block (423) is provided with an inclined surface, and a rubber pad is laid on the clamping block (423).

6. A cell separation, extraction and processing device according to claim 1, characterized in that: A protective cover is inserted into the upper end of the bottom plate (1), the protective cover is made of glass, and a handle is arranged on the upper end of the protective cover.

7. A cell separation, extraction and processing device according to claim 4, characterized in that: Glass plates are symmetrically mounted on the side walls of the thermostatic sleeve (33), scale bars are arranged on the side walls of the thermostatic sleeve (33) near the glass plates, and a thermostatic ring is arranged on the inner wall of the thermostatic sleeve (33).

8. A cell separation, extraction and processing device according to claim 1, characterized in that: A sealing ring is sleeved on the side wall of the dense hole plate (409).

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